MC06g1318 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1318
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein NRT1/ PTR FAMILY 6.3-like
LocationMC06: 20133813 .. 20137500 (+)
RNA-Seq ExpressionMC06g1318
SyntenyMC06g1318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTTGTGCTATAAAAAGGGTCCAAGTTCGTGCAGAATCTGCATCCCAGTTTGCCACATATCATATCCATTTCAAACTCCCTTCTTCTTCATCAGCCCAAAGCCACACCATGAGCATTCTCCCTCAAGCTCATGCTCAAGCTCTAGAGAAGACTCTCCCAGATGCTTGGGACTACACCGGCAAACCCGCTTCCCGGGCCAGAACCGGTGGCTGGGTCGCCGCTGCCATGATTCTAGGTTTGTCCCGTTCTCGTTCTTTCTTCCTTTGTTATATTCAGTGGCAGGTCTAAAAATTTTATTAACAAACACCAAAATATAAAGGAAAAAAGAAAGCTTAGCATTTATAACACTTCAATTATACCATTGTTTGAGAGATTATCCTCTTCTTGAATTTTACGCTCCTCTATCTCACCATCAATAAAAATATGATAGTTTTGTGATTTTTTTGGAAAAAAAAAATATAACTTTCTTTTGTATTTCGCTTTCTTATTCGTAAAAACTTGAACGGTAGTTAATAAGTACTTAAAAGGTATTTTTCTCTCCTTCCTTCTTTGTTCGGCCTGTTTTAACGTGGGTTTTTTTGGGTTGACGATGATATTAAAACGCTGCGTTTTGGCAGGGACTGAGGCCGTGGAGAGGCTGACGACGTTGGGGATCGCCGTGAATTTGGTGACGTATTTAACGGAAACAATGCATCTCGGGAATGCTACTTCGGCCAACGATGTCACGAACTTCCTCGGCACTTCCTTCATGCTCTGTTTGCTCGGCGGTTTTGTCGCCGACACTTTTCTCGGCCGGTAAATTTATTAATTAATTAATTATATTTCCTTTTTTCTTTTCTTTTTCTTTTTTTTCTCCTTCTAACTCAATTTATTTATTTCCATGGTTTTTCTTTTCTGATTTATTTTATCTCTTTCTTCTCAAAGGTATCTCACCATTTCCATTTTCGCAGCTGTTCAAGCCACGGTAATTAAGATTTACTTTACTGTGTCTAATTTTCTTTATTTATTTATTTTTATTTTTTAACAAAAAAAAAACTAGGACCTCATTTGAGAATTTAAAAAAACCAAGTTTATTCAGCCAAAATATGTTTTTGAATTAATCTAAATTTTGTGGCATTAGGGTGTAACTATCTTGACAATCTCCACCATAATTCCAAGTCTCCGACCTCCGAAATGTTCACTGGGAGTCCCCAACCCCCCATGCATTCCGGCGAACGGCAAGCAGCTGGCTATTCTCTACCTAGCACTATACCTGTTGGCGCTCGGCACCGGCGGCCTAAAATCGAGCGTCTCCGGCTTCGGTTCCGATCAGTTCGACGACTCCGACAAAGAGGAAAGAGGGAAGATGAGCAAGTTCTTCAACTGGTTCTTCTTCTTCATAAGCATCGGCTCACTTGGAGCTGTGACAATTCTCGTTTACATTCAAGACAACGTGGGCAGACAATGGGGTTATGGGATATGTGCATGTGCCATTGTGTTGGGTTTGATCGTGTTCCTGTCGGGAACTAGACGGTACCGTTTTAAGAAATTGGTTGGCAGCCCGTTGACGCAGATTGCCACTGTGTTCATTGCAGCGTGGAAGAAGAGGAGCTTGGAGTTGCCGTCTGATCCGTTTTTGTTGTTCGACATTGATGACAATGTCGACGATGGAGCTACGAAGAAGAAGAAGCAGAAGCTCCCCCGCACCAAACAATTCAGGTTGGTTTTTTCTACGTATGGAATATTACTTATTAATCGTAATTCTTTTGGTAGGTTTTTCCCTATAAAGAATCTTTTCAAATTTTGCCTTAAATTTTGAGAAAATTTTTAAAAGTAAATAGCAAAATAATGAGACACAAGGTAGAAGTAACATTTCTAGGTTTGATTTTTAAAAAATGAAACAAAAAACGAATGGTTATCCCATGGATCTTCACATTTAAAATTTAATTGTTTGTTTTATTAAATTTATGCTACGAAGTAGAATTTTTTAACAAAAAAAAATCACAAACCCATTTCCTAAAATTGAAAAATCATAATAATACAATCAATATTTAATGAAATATAGTCAACATAGTTGGTATAATGTAATCGAAAATATAATTTAGATAATCATGCACTAAACTTGCGATTACATAGAGGCCAAAAAATCCAAGCCAAATTCTACCTTTGGCCACGTATTATGCTCTATAATAATAAGTTAAAAACTATATCCTAAACCAACAGGAAGCAGACAAGCTACAAAAAACTTTTTCTACTCTTGGAAGTTACCCATAATTGAGGCTTTTATATTCGTTATTTTCAATTTGCAGACAAGAGAGAAGAATATTTTTATTCGCTACAAAATGTTTGGACACAACTCTTCTTCAACATTTAGCCGACAATATAGTTGATGATTTTATACTATACTACTATTATTGTGTCTTAATCATGCATCCAATGGTTAAAAGAGAACAATACATTCAAATAATTTTCTATTTAAATCGAATTGAAAAGACCTATTGAACATGTTATTAATTTGGGCATATTCAGGTTTTTGGACAAGGCAGCAATCAAGGATGGAGAAATTGTTGGTAATGTGGTAAATAAGTGGAACATGTCAACCCTAACAGACATAGAGGAAGTAAAATTAGTGATTAGAATGTTACCCATTTGGGCAACCACCATTCTTTTCTGGACTGTTTATGCCCAAATGACAACTTTATCTGTGTCACAAGCCACCACAATGAACCGGCACATCGGAAAATCGTTCCAAATTCCAGCGGCCTCTCTCACCGTCTTCTTCGTCGCCAGCACCCTCTTCACCGTTCCGATTTATGATCGATTCATCGTCCCAATAACATCGAAACTGCTTAAGAACCCACAAGGCCTGACCTCACTGCAGCGGATCGGGGTCGGCTTGGTTATGTCGATACTCGCCATGGTCACCGCCGCACTGACCGAGATAAAGCGACTGAGGGCGGTGGAGGCAAATGGGTTGGTAGAGAAGCCCTTGGAAGAAGTTCCATTGAGCGTGTTCTGGTTGGCACCACAATTTTTACTGGTGGGATCAGGTGAGTCAAAGTTACCTCTTTAATTTACCTTACCGTAAATTCAATACATACACAGTTAGAATGTAAAATTTTGTCTCTTCCTCGAACTCGACCCTACAGGTGAGGCATTCACGTACATGGGGCAGTTGGATTTCTTCCTGAGAGAGTGTCCCAAGGGGATGAAGACAATGAGCACAGGGCTATTTCTAAGCACACTTTCATTAGGGTTTTTCTTCAGCACATTGTTGGTCACAATTGTGCAGAAAGTGACTGCACATGGGAAGCCATGGATTCCAGACAATCTGAACAAAGGGAAGCTTTACAATTTCTATTGGCTTTTGGCAATTTTGAGTGTGCTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGATGAGGGGATTGAATTGGAGGACTCTGAACCAACTTTCCATGCATAGCATAATAATAATCACTCTCTCTTGTCTTCTTTTTTTTGTGTGGGTTTCTTAAGTATAAGTATAAAAGTTTTAAGCCCCTTTTTTAATATTGGGTTACCTTTTGCCTTTTTAATAATGTAAAGTTTGTAAATTTTGATGTACCAAAAGCATGGGAAAGTGAGAATCACTTTTGAATAATATAATATCACGTTTGGGTGTTTTTTGTTTCCCTCA

mRNA sequence

AGTTGTGCTATAAAAAGGGTCCAAGTTCGTGCAGAATCTGCATCCCAGTTTGCCACATATCATATCCATTTCAAACTCCCTTCTTCTTCATCAGCCCAAAGCCACACCATGAGCATTCTCCCTCAAGCTCATGCTCAAGCTCTAGAGAAGACTCTCCCAGATGCTTGGGACTACACCGGCAAACCCGCTTCCCGGGCCAGAACCGGTGGCTGGGTCGCCGCTGCCATGATTCTAGGGACTGAGGCCGTGGAGAGGCTGACGACGTTGGGGATCGCCGTGAATTTGGTGACGTATTTAACGGAAACAATGCATCTCGGGAATGCTACTTCGGCCAACGATGTCACGAACTTCCTCGGCACTTCCTTCATGCTCTGTTTGCTCGGCGGTTTTGTCGCCGACACTTTTCTCGGCCGGTATCTCACCATTTCCATTTTCGCAGCTGTTCAAGCCACGGGTGTAACTATCTTGACAATCTCCACCATAATTCCAAGTCTCCGACCTCCGAAATGTTCACTGGGAGTCCCCAACCCCCCATGCATTCCGGCGAACGGCAAGCAGCTGGCTATTCTCTACCTAGCACTATACCTGTTGGCGCTCGGCACCGGCGGCCTAAAATCGAGCGTCTCCGGCTTCGGTTCCGATCAGTTCGACGACTCCGACAAAGAGGAAAGAGGGAAGATGAGCAAGTTCTTCAACTGGTTCTTCTTCTTCATAAGCATCGGCTCACTTGGAGCTGTGACAATTCTCGTTTACATTCAAGACAACGTGGGCAGACAATGGGGTTATGGGATATGTGCATGTGCCATTGTGTTGGGTTTGATCGTGTTCCTGTCGGGAACTAGACGGTACCGTTTTAAGAAATTGGTTGGCAGCCCGTTGACGCAGATTGCCACTGTGTTCATTGCAGCGTGGAAGAAGAGGAGCTTGGAGTTGCCGTCTGATCCGTTTTTGTTGTTCGACATTGATGACAATGTCGACGATGGAGCTACGAAGAAGAAGAAGCAGAAGCTCCCCCGCACCAAACAATTCAGGTTTTTGGACAAGGCAGCAATCAAGGATGGAGAAATTGTTGGTAATGTGGTAAATAAGTGGAACATGTCAACCCTAACAGACATAGAGGAAGTAAAATTAGTGATTAGAATGTTACCCATTTGGGCAACCACCATTCTTTTCTGGACTGTTTATGCCCAAATGACAACTTTATCTGTGTCACAAGCCACCACAATGAACCGGCACATCGGAAAATCGTTCCAAATTCCAGCGGCCTCTCTCACCGTCTTCTTCGTCGCCAGCACCCTCTTCACCGTTCCGATTTATGATCGATTCATCGTCCCAATAACATCGAAACTGCTTAAGAACCCACAAGGCCTGACCTCACTGCAGCGGATCGGGGTCGGCTTGGTTATGTCGATACTCGCCATGGTCACCGCCGCACTGACCGAGATAAAGCGACTGAGGGCGGTGGAGGCAAATGGGTTGGTAGAGAAGCCCTTGGAAGAAGTTCCATTGAGCGTGTTCTGGTTGGCACCACAATTTTTACTGGTGGGATCAGGTGAGGCATTCACGTACATGGGGCAGTTGGATTTCTTCCTGAGAGAGTGTCCCAAGGGGATGAAGACAATGAGCACAGGGCTATTTCTAAGCACACTTTCATTAGGGTTTTTCTTCAGCACATTGTTGGTCACAATTGTGCAGAAAGTGACTGCACATGGGAAGCCATGGATTCCAGACAATCTGAACAAAGGGAAGCTTTACAATTTCTATTGGCTTTTGGCAATTTTGAGTGTGCTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGATGAGGGGATTGAATTGGAGGACTCTGAACCAACTTTCCATGCATAGCATAATAATAATCACTCTCTCTTGTCTTCTTTTTTTTGTGTGGGTTTCTTAAGTATAAGTATAAAAGTTTTAAGCCCCTTTTTTAATATTGGGTTACCTTTTGCCTTTTTAATAATGTAAAGTTTGTAAATTTTGATGTACCAAAAGCATGGGAAAGTGAGAATCACTTTTGAATAATATAATATCACGTTTGGGTGTTTTTTGTTTCCCTCA

Coding sequence (CDS)

AGTTGTGCTATAAAAAGGGTCCAAGTTCGTGCAGAATCTGCATCCCAGTTTGCCACATATCATATCCATTTCAAACTCCCTTCTTCTTCATCAGCCCAAAGCCACACCATGAGCATTCTCCCTCAAGCTCATGCTCAAGCTCTAGAGAAGACTCTCCCAGATGCTTGGGACTACACCGGCAAACCCGCTTCCCGGGCCAGAACCGGTGGCTGGGTCGCCGCTGCCATGATTCTAGGGACTGAGGCCGTGGAGAGGCTGACGACGTTGGGGATCGCCGTGAATTTGGTGACGTATTTAACGGAAACAATGCATCTCGGGAATGCTACTTCGGCCAACGATGTCACGAACTTCCTCGGCACTTCCTTCATGCTCTGTTTGCTCGGCGGTTTTGTCGCCGACACTTTTCTCGGCCGGTATCTCACCATTTCCATTTTCGCAGCTGTTCAAGCCACGGGTGTAACTATCTTGACAATCTCCACCATAATTCCAAGTCTCCGACCTCCGAAATGTTCACTGGGAGTCCCCAACCCCCCATGCATTCCGGCGAACGGCAAGCAGCTGGCTATTCTCTACCTAGCACTATACCTGTTGGCGCTCGGCACCGGCGGCCTAAAATCGAGCGTCTCCGGCTTCGGTTCCGATCAGTTCGACGACTCCGACAAAGAGGAAAGAGGGAAGATGAGCAAGTTCTTCAACTGGTTCTTCTTCTTCATAAGCATCGGCTCACTTGGAGCTGTGACAATTCTCGTTTACATTCAAGACAACGTGGGCAGACAATGGGGTTATGGGATATGTGCATGTGCCATTGTGTTGGGTTTGATCGTGTTCCTGTCGGGAACTAGACGGTACCGTTTTAAGAAATTGGTTGGCAGCCCGTTGACGCAGATTGCCACTGTGTTCATTGCAGCGTGGAAGAAGAGGAGCTTGGAGTTGCCGTCTGATCCGTTTTTGTTGTTCGACATTGATGACAATGTCGACGATGGAGCTACGAAGAAGAAGAAGCAGAAGCTCCCCCGCACCAAACAATTCAGGTTTTTGGACAAGGCAGCAATCAAGGATGGAGAAATTGTTGGTAATGTGGTAAATAAGTGGAACATGTCAACCCTAACAGACATAGAGGAAGTAAAATTAGTGATTAGAATGTTACCCATTTGGGCAACCACCATTCTTTTCTGGACTGTTTATGCCCAAATGACAACTTTATCTGTGTCACAAGCCACCACAATGAACCGGCACATCGGAAAATCGTTCCAAATTCCAGCGGCCTCTCTCACCGTCTTCTTCGTCGCCAGCACCCTCTTCACCGTTCCGATTTATGATCGATTCATCGTCCCAATAACATCGAAACTGCTTAAGAACCCACAAGGCCTGACCTCACTGCAGCGGATCGGGGTCGGCTTGGTTATGTCGATACTCGCCATGGTCACCGCCGCACTGACCGAGATAAAGCGACTGAGGGCGGTGGAGGCAAATGGGTTGGTAGAGAAGCCCTTGGAAGAAGTTCCATTGAGCGTGTTCTGGTTGGCACCACAATTTTTACTGGTGGGATCAGGTGAGGCATTCACGTACATGGGGCAGTTGGATTTCTTCCTGAGAGAGTGTCCCAAGGGGATGAAGACAATGAGCACAGGGCTATTTCTAAGCACACTTTCATTAGGGTTTTTCTTCAGCACATTGTTGGTCACAATTGTGCAGAAAGTGACTGCACATGGGAAGCCATGGATTCCAGACAATCTGAACAAAGGGAAGCTTTACAATTTCTATTGGCTTTTGGCAATTTTGAGTGTGCTGAATTTGGTGGTGTTTTTGTTTTGTGCAAAGTGGTATGTGTACAAGGACAAGAGGTTGGCTGATGAGGGGATTGAATTGGAGGACTCTGAACCAACTTTCCATGCATAG

Protein sequence

SCAIKRVQVRAESASQFATYHIHFKLPSSSSAQSHTMSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPDNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA
Homology
BLAST of MC06g1318 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 1.2e-213
Identity = 382/580 (65.86%), Postives = 463/580 (79.83%), Query Frame = 0

Query: 52  LPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSA 111
           L DAWD+ G+PA R++TGGW +AAMIL  EAVERLTTLGI VNLVTYLT TMHLGNAT+A
Sbjct: 12  LLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAA 71

Query: 112 NDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCS 171
           N VTNFLGTSFMLCLLGGF+ADTFLGRYLTI+IFAA+QATGV+ILT+STIIP LRPP+C+
Sbjct: 72  NTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCN 131

Query: 172 LGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFF 231
               +  C  A+G QL +LYLALYL ALGTGG+K+SVSGFGSDQFD+++ +ER KM+ FF
Sbjct: 132 -PTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFF 191

Query: 232 NWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGS 291
           N FFF I++GSL AVT+LVY+QD+VGR+WGYGICA AIVL L VFL+GT RYRFKKL+GS
Sbjct: 192 NRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGS 251

Query: 292 PLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAI 351
           P+TQ+A V +AAW+ R LELP+DP  L+D+DD +    + K KQKLP T+QFR LDKAAI
Sbjct: 252 PMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI 311

Query: 352 KDGE--IVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTM 411
           +D E  +  NV NKW +STLTD+EEVK ++RMLPIWAT ILFWTV+AQ+TTLSV+Q+ T+
Sbjct: 312 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 371

Query: 412 NRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVM 471
           +R IG SF+IP AS+ VF+V   L T  +YDR  + +  KL   P GL  LQRIG+GL  
Sbjct: 372 DRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFF 431

Query: 472 SILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDF 531
             +AM  AAL E+KRLR   A+G   K L   PL  + L PQ+L+VG GEA  Y GQLDF
Sbjct: 432 GSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEALIYTGQLDF 491

Query: 532 FLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPDNLNKGKLYNFYW 591
           FLRECPKGMK MSTGL LSTL+LGFFFS++LVTIV+K T    PWI D+LNKG+LYNFYW
Sbjct: 492 FLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYW 551

Query: 592 LLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPT 630
           L+A+L  LN ++FL  +KWYVYK+KRLA+ GIEL+D EP+
Sbjct: 552 LVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD-EPS 585

BLAST of MC06g1318 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.2e-168
Identity = 317/567 (55.91%), Postives = 412/567 (72.66%), Query Frame = 0

Query: 51  TLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATS 110
           T+ DA DY G+PA +++TGGW+ AA+ILG E VERL+T+GIAVNLVTYL ETMHL ++TS
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 111 ANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKC 170
           AN VT+F+GTSF+LCLLGGF+AD+FLGR+ TI IF+ +QA G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 171 SLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKF 230
             G     CIPA   Q+ ILY++LYL+ALGTGGLKSS+SGFGSDQFDD D +E+  M+ F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 231 FNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVG 290
           FN FFFFIS+G+L AVT+LVY+QD VGR W YGIC  ++ + +++FL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 291 SPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAA 350
           SP+ QI  V  AA++KR +ELP     L+  +DN +         ++  T QF  LDKAA
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELPQSIVYLY--EDNPEG-------IRIEHTDQFHLLDKAA 307

Query: 351 I-KDGEIVGN-----VVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVS 410
           I  +G+         + N W +S++T +EEVK+++R+LPIWATTI+FWT YAQM T SV 
Sbjct: 308 IVAEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVE 367

Query: 411 QATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIG 470
           QA+TM R+IG SF+IPA SLTVFFVA+ L T+ +YDR I+P   K  K   G +SLQRI 
Sbjct: 368 QASTMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIA 427

Query: 471 VGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYM 530
           +GLV+S   M  AAL E KRL   +++       + +P+SVF L PQF LVG+GEAF Y 
Sbjct: 428 IGLVLSTAGMAAAALVEQKRLSVAKSSS-----QKTLPISVFLLVPQFFLVGAGEAFIYT 487

Query: 531 GQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKP--WIPDNLNKG 590
           GQLDFF+ + PKGMKTMSTGLFL+TLSLGFF S+ LV+IV++VT+      W+ DN+N G
Sbjct: 488 GQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHG 547

Query: 591 KLYNFYWLLAILSVLNLVVFLFCAKWY 610
           +L  FYWLL ILS +N VV++ CA W+
Sbjct: 548 RLDYFYWLLVILSGINFVVYIICALWF 555

BLAST of MC06g1318 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 4.0e-148
Identity = 290/565 (51.33%), Postives = 381/565 (67.43%), Query Frame = 0

Query: 54  DAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSAND 113
           +A+DY G P  +++TGGW+ A +ILG+E  ER+  +GI++NLVTYL   +H+ +A SA  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 114 VTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKC-SL 173
           VTNF+GT  +L LLGGF+AD  LGRY  ++I A+V A GV +LT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 174 GVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFN 233
              +  CI ANG QLA+LY+ALY +ALG GG+KS+VSGFGSDQFD SD +E  +M  FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 234 WFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSP 293
            F+F IS+GSL AV  LVY+QDNVGR WGYGI A  +V+  IV L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 294 LTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAIK 353
            T I  V   AWKKR    P+ P LL   D+             +P T+  + LDKAAI 
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTT-----------VPHTEMLKCLDKAAIS 315

Query: 354 DGEIVGN-----VVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQAT 413
             E   +       + W +ST+T +EEVKLV++++PIWAT ILFWT+Y+QMTT +V QAT
Sbjct: 316 KNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQAT 375

Query: 414 TMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGL 473
            M+R +G SF +PA S + F + + L    + +R  VP+T +L K PQG+TSLQRIGVGL
Sbjct: 376 FMDRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGL 435

Query: 474 VMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQL 533
           V S+ AM  AA+ E  R  A   N        +  +S FWL PQ+ LVG+GEAF Y+GQL
Sbjct: 436 VFSMAAMAVAAVIENARREAAVNN--------DKKISAFWLVPQYFLVGAGEAFAYVGQL 495

Query: 534 DFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPDNLNKGKLYNF 593
           +FF+RE P+ MK+MSTGLFLST+S+GFF S+LLV++V +VT   K W+  NLNK +L  F
Sbjct: 496 EFFIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVT--DKSWLRSNLNKARLNYF 555

Query: 594 YWLLAILSVLNLVVFLFCAKWYVYK 613
           YWLL +L  LN ++F+  A  + YK
Sbjct: 556 YWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of MC06g1318 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.2e-139
Identity = 260/565 (46.02%), Postives = 386/565 (68.32%), Query Frame = 0

Query: 57  DYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSANDVTN 116
           D    PA++ +TG W A   ILG E  ERL   G+  NLV YL   ++ GNAT+AN+VTN
Sbjct: 14  DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTN 73

Query: 117 FLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCSLGVPN 176
           + GT ++  L+G F+AD +LGRY TI+ F  +  +G+T+LT+S  +P L+P  C+    +
Sbjct: 74  WSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNADTCH 133

Query: 177 PPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFNWFFF 236
           P     N  Q A+ ++ALY++ALGTGG+K  VS FG+DQFD++D+ E+ K S FFNWF+F
Sbjct: 134 P-----NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYF 193

Query: 237 FISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSPLTQI 296
            I++G+L A T+LV+IQ NVG  WG+G+   A+V+ +  F  G+R YR ++  GSPLT+I
Sbjct: 194 SINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRI 253

Query: 297 ATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAA------ 356
             V +AA++K S+++P D  LLF   +  DD +  K  +KL  T   +F DKAA      
Sbjct: 254 FQVIVAAFRKISVKVPEDKSLLF---ETADDESNIKGSRKLVHTDNLKFFDKAAVESQSD 313

Query: 357 -IKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTM 416
            IKDGE     VN W + ++T +EE+K +I +LP+WAT I+F TVY+QM+T+ V Q  TM
Sbjct: 314 SIKDGE-----VNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 373

Query: 417 NRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVM 476
           ++H+GK+F+IP+ASL++F   S LF  P+YD+FI+P+  K  +N +G T LQR+G+GLV+
Sbjct: 374 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 433

Query: 477 SILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDF 536
           SI AM+TA + E+ RL  V+ +   ++  +++ +S+FW  PQ+LL+G  E FT++GQL+F
Sbjct: 434 SIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 493

Query: 537 FLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVT-AHGKP-WIPDNLNKGKLYNF 596
           F  + P  M+++ + L L+T++LG + ST+LVT+V K+T  +GKP WIPDNLN+G L  F
Sbjct: 494 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYF 553

Query: 597 YWLLAILSVLNLVVFLFCAKWYVYK 613
           ++LLA LS LN +V+L+ +K Y YK
Sbjct: 554 FYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of MC06g1318 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.9e-127
Identity = 247/560 (44.11%), Postives = 369/560 (65.89%), Query Frame = 0

Query: 57  DYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSANDVTN 116
           D   KPA++ +TG W A   ILGTE  ERL   G++ NL+ YL + M++ N +++  V+N
Sbjct: 15  DIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSN 74

Query: 117 FLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCSLGVPN 176
           + GT +   L+G F+AD +LGRY TI+ F  +   G+T+LTIS  +P L  P CS     
Sbjct: 75  WSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS----G 134

Query: 177 PPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFNWFFF 236
             C  A   Q AI ++ALYL+ALGTGG+K  VS FG+DQFDD+D++E+   S FFNWF+F
Sbjct: 135 ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYF 194

Query: 237 FISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSPLTQI 296
            I++G++ A ++LV+IQ NVG  WG G+   A+ + ++ F +G+  YR +K  GSPLT++
Sbjct: 195 VINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRM 254

Query: 297 ATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAIK-DGE 356
             V +A+ +K  +++P D  LL+   +N D  ++    +KL  TK   F DKAA++ + +
Sbjct: 255 LQVIVASCRKSKVKIPEDESLLY---ENQDAESSIIGSRKLEHTKILTFFDKAAVETESD 314

Query: 357 IVGNV-VNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTMNRHIG 416
             G    + W + T+T +EE+K +IR+LPIWAT I+F +VY+QM T+ V Q  T+++H+G
Sbjct: 315 NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMG 374

Query: 417 KSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVMSILAM 476
            +F+IP+ASL++F   S LF  P+YD+ IVP   K   + +G T LQRIG+GLV+SI +M
Sbjct: 375 PNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSM 434

Query: 477 VTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDFFLREC 536
           V+A + E+ RL  V+ + L  +  E +P+++FW  PQ+ LVG  E FT++GQL+FF  + 
Sbjct: 435 VSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 494

Query: 537 PKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVT-AHGKP-WIPDNLNKGKLYNFYWLLA 596
           P  M+++ + L L+ ++ G + ST LVT+V KVT + G+P WI  NLN G L  F+WLLA
Sbjct: 495 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 554

Query: 597 ILSVLNLVVFLFCAKWYVYK 613
            LS LN +V+L+ AKWY YK
Sbjct: 555 GLSFLNFLVYLWIAKWYTYK 563

BLAST of MC06g1318 vs. NCBI nr
Match: XP_022156448.1 (protein NRT1/ PTR FAMILY 6.3-like isoform X1 [Momordica charantia])

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 591/596 (99.16%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 37  MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 96
           MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV
Sbjct: 1   MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 60

Query: 97  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 156
           TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL
Sbjct: 61  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 120

Query: 157 TISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 216
           TISTIIPSLRPPKCS  + N PCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF
Sbjct: 121 TISTIIPSLRPPKCSPELTNTPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 180

Query: 217 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 276
           DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF
Sbjct: 181 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 240

Query: 277 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 336
           LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK
Sbjct: 241 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 300

Query: 337 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 396
           LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA
Sbjct: 301 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 360

Query: 397 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 456
           QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG
Sbjct: 361 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 420

Query: 457 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 516
           LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG
Sbjct: 421 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 480

Query: 517 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 576
           SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP
Sbjct: 481 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 540

Query: 577 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 632
           DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA
Sbjct: 541 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 596

BLAST of MC06g1318 vs. NCBI nr
Match: XP_022156449.1 (protein NRT1/ PTR FAMILY 6.3-like isoform X2 [Momordica charantia])

HSP 1 Score: 1110 bits (2871), Expect = 0.0
Identity = 570/596 (95.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 37  MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 96
           MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV
Sbjct: 1   MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 60

Query: 97  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 156
           TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL
Sbjct: 61  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 120

Query: 157 TISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 216
           TISTIIPSLRPPKCS  + N PCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF
Sbjct: 121 TISTIIPSLRPPKCSPELTNTPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 180

Query: 217 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 276
           DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF
Sbjct: 181 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 240

Query: 277 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 336
           LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK
Sbjct: 241 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 300

Query: 337 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 396
           LPRTKQFRFLDKAAIKDGEI GNVVNKWN+STLTDIEEVKLVIRMLPIWATTILFWT+YA
Sbjct: 301 LPRTKQFRFLDKAAIKDGEIFGNVVNKWNISTLTDIEEVKLVIRMLPIWATTILFWTIYA 360

Query: 397 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 456
           QMTT SVSQATTMNRH+G+SFQIPAASLTVFFVAS L TVPIYDRFI+PIT+KLL+NPQG
Sbjct: 361 QMTTFSVSQATTMNRHVGRSFQIPAASLTVFFVASILLTVPIYDRFIIPITAKLLRNPQG 420

Query: 457 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 516
           L+ LQR+G+GLV+SILAMV AALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG
Sbjct: 421 LSPLQRVGLGLVLSILAMVAAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 480

Query: 517 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 576
           SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTA+GKPWIP
Sbjct: 481 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTANGKPWIP 540

Query: 577 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 632
           DNLNKGKLY+FYWLLAILSVLNLV FLFCAKWYVYKDKRLADEGIELEDSEPT HA
Sbjct: 541 DNLNKGKLYDFYWLLAILSVLNLVAFLFCAKWYVYKDKRLADEGIELEDSEPTCHA 596

BLAST of MC06g1318 vs. NCBI nr
Match: XP_004147617.1 (protein NRT1/ PTR FAMILY 6.3 [Cucumis sativus] >KGN50229.1 hypothetical protein Csa_000452 [Cucumis sativus])

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 502/594 (84.51%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +A+TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKAKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQA GVTILT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAAGVTILT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMSKFFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIVLGL+ FL
Sbjct: 183 DSDKEERAKMSKFFNWFFFIISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVAFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQI  VF AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIVAVFYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQATTMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFI+PI S++LKNPQGL
Sbjct: 363 MTTFSVSQATTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIIPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AMV AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMVAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LGFFFS+LLVTIV KVT HG+PWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGRPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLAILSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAILSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. NCBI nr
Match: KAA0039694.1 (protein NRT1/ PTR FAMILY 6.3-like [Cucumis melo var. makuwa])

HSP 1 Score: 973 bits (2516), Expect = 0.0
Identity = 499/594 (84.01%), Postives = 539/594 (90.74%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +++TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQATGVT+LT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMS FFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIV+GL+VFL
Sbjct: 183 DSDKEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQIA V  AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQA+TMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFIVPI S++LKNPQGL
Sbjct: 363 MTTFSVSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AM+ AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LGFFFS+LLVTIV KVT HGKPWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGKPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLA+LSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. NCBI nr
Match: XP_008437115.1 (PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like [Cucumis melo])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 498/594 (83.84%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +++TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQATGVT+LT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMS FFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIV+GL+VFL
Sbjct: 183 DSDKEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQIA V  AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQA+TMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFIVPI S++LKNPQGL
Sbjct: 363 MTTFSVSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AM+ AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LG FFS+LLVTIV KVT HGKPWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGXFFSSLLVTIVGKVTEHGKPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLA+LSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. ExPASy TrEMBL
Match: A0A6J1DQN1 (protein NRT1/ PTR FAMILY 6.3-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023339 PE=3 SV=1)

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 591/596 (99.16%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 37  MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 96
           MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV
Sbjct: 1   MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 60

Query: 97  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 156
           TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL
Sbjct: 61  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 120

Query: 157 TISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 216
           TISTIIPSLRPPKCS  + N PCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF
Sbjct: 121 TISTIIPSLRPPKCSPELTNTPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 180

Query: 217 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 276
           DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF
Sbjct: 181 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 240

Query: 277 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 336
           LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK
Sbjct: 241 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 300

Query: 337 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 396
           LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA
Sbjct: 301 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 360

Query: 397 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 456
           QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG
Sbjct: 361 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 420

Query: 457 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 516
           LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG
Sbjct: 421 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 480

Query: 517 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 576
           SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP
Sbjct: 481 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 540

Query: 577 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 632
           DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA
Sbjct: 541 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 596

BLAST of MC06g1318 vs. ExPASy TrEMBL
Match: A0A6J1DTH4 (protein NRT1/ PTR FAMILY 6.3-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023339 PE=3 SV=1)

HSP 1 Score: 1110 bits (2871), Expect = 0.0
Identity = 570/596 (95.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 37  MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 96
           MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV
Sbjct: 1   MSILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLV 60

Query: 97  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 156
           TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL
Sbjct: 61  TYLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTIL 120

Query: 157 TISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 216
           TISTIIPSLRPPKCS  + N PCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF
Sbjct: 121 TISTIIPSLRPPKCSPELTNTPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQF 180

Query: 217 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 276
           DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF
Sbjct: 181 DDSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVF 240

Query: 277 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 336
           LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK
Sbjct: 241 LSGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQK 300

Query: 337 LPRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYA 396
           LPRTKQFRFLDKAAIKDGEI GNVVNKWN+STLTDIEEVKLVIRMLPIWATTILFWT+YA
Sbjct: 301 LPRTKQFRFLDKAAIKDGEIFGNVVNKWNISTLTDIEEVKLVIRMLPIWATTILFWTIYA 360

Query: 397 QMTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQG 456
           QMTT SVSQATTMNRH+G+SFQIPAASLTVFFVAS L TVPIYDRFI+PIT+KLL+NPQG
Sbjct: 361 QMTTFSVSQATTMNRHVGRSFQIPAASLTVFFVASILLTVPIYDRFIIPITAKLLRNPQG 420

Query: 457 LTSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 516
           L+ LQR+G+GLV+SILAMV AALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG
Sbjct: 421 LSPLQRVGLGLVLSILAMVAAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVG 480

Query: 517 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIP 576
           SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTA+GKPWIP
Sbjct: 481 SGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTANGKPWIP 540

Query: 577 DNLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFHA 632
           DNLNKGKLY+FYWLLAILSVLNLV FLFCAKWYVYKDKRLADEGIELEDSEPT HA
Sbjct: 541 DNLNKGKLYDFYWLLAILSVLNLVAFLFCAKWYVYKDKRLADEGIELEDSEPTCHA 596

BLAST of MC06g1318 vs. ExPASy TrEMBL
Match: A0A0A0KMV3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G161290 PE=3 SV=1)

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 502/594 (84.51%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +A+TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKAKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQA GVTILT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQAAGVTILT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMSKFFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIVLGL+ FL
Sbjct: 183 DSDKEERAKMSKFFNWFFFIISIGSLGAVTILVYIQDNWGRQWGYGICACAIVLGLVAFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQI  VF AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIVAVFYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQATTMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFI+PI S++LKNPQGL
Sbjct: 363 MTTFSVSQATTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIIPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AMV AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMVAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LGFFFS+LLVTIV KVT HG+PWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGRPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLAILSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAILSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. ExPASy TrEMBL
Match: A0A5A7T897 (Protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00250 PE=3 SV=1)

HSP 1 Score: 973 bits (2516), Expect = 0.0
Identity = 499/594 (84.01%), Postives = 539/594 (90.74%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +++TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQATGVT+LT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMS FFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIV+GL+VFL
Sbjct: 183 DSDKEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQIA V  AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQA+TMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFIVPI S++LKNPQGL
Sbjct: 363 MTTFSVSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AM+ AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LGFFFS+LLVTIV KVT HGKPWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSLLVTIVGKVTEHGKPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLA+LSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. ExPASy TrEMBL
Match: A0A1S3ATS1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo OX=3656 GN=LOC103482632 PE=3 SV=1)

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 498/594 (83.84%), Postives = 538/594 (90.57%), Query Frame = 0

Query: 38  SILPQAHAQALEKTLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVT 97
           S+LP+      EKTLPDAWDYTGKPA +++TGGWVAAAMILG EAVERLTTLGIAVNLVT
Sbjct: 3   SVLPET---IQEKTLPDAWDYTGKPAIKSKTGGWVAAAMILGPEAVERLTTLGIAVNLVT 62

Query: 98  YLTETMHLGNATSANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILT 157
           YLTETMHLGNAT+AN+VTNFLGTSFMLCL GGFVADTFLGRYLTISIFA VQATGVT+LT
Sbjct: 63  YLTETMHLGNATAANNVTNFLGTSFMLCLFGGFVADTFLGRYLTISIFATVQATGVTLLT 122

Query: 158 ISTIIPSLRPPKCSLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFD 217
           ISTIIPSLRPPKCS GV   PCI A+ KQLA+L+ ALYL ALGTGGLKSSVSGFGSDQFD
Sbjct: 123 ISTIIPSLRPPKCSPGVSTTPCIMADSKQLAVLHTALYLTALGTGGLKSSVSGFGSDQFD 182

Query: 218 DSDKEERGKMSKFFNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFL 277
           DSDKEER KMS FFNWFFF ISIGSLGAVTILVYIQDN GRQWGYGICACAIV+GL+VFL
Sbjct: 183 DSDKEERVKMSNFFNWFFFLISIGSLGAVTILVYIQDNWGRQWGYGICACAIVMGLVVFL 242

Query: 278 SGTRRYRFKKLVGSPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKL 337
            GTR+YRFKKLVGSPLTQIA V  AAW+KR L+LPSDPF L+ I+D VD     KKKQKL
Sbjct: 243 LGTRKYRFKKLVGSPLTQIAAVIYAAWRKRKLDLPSDPFSLYQIEDAVDGSG--KKKQKL 302

Query: 338 PRTKQFRFLDKAAIKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQ 397
           PRTKQFRFLDKAAIKD E+VGNVVNKW +STLTDIEEVKLV+RMLPIWATTI+FWTVYAQ
Sbjct: 303 PRTKQFRFLDKAAIKDEEVVGNVVNKWKISTLTDIEEVKLVLRMLPIWATTIIFWTVYAQ 362

Query: 398 MTTLSVSQATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGL 457
           MTT SVSQA+TMNRH+GKSF+IPAASLTVFFVAS L TVPIYDRFIVPI S++LKNPQGL
Sbjct: 363 MTTFSVSQASTMNRHMGKSFEIPAASLTVFFVASILLTVPIYDRFIVPIASRILKNPQGL 422

Query: 458 TSLQRIGVGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGS 517
           + LQR+GVGLV+SI+AM+ AALTEIKRL+ VE NGL  KP  EVPLSVFWLAPQFLLVGS
Sbjct: 423 SPLQRVGVGLVLSIIAMIAAALTEIKRLKVVEENGLTYKPTAEVPLSVFWLAPQFLLVGS 482

Query: 518 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPD 577
           GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTL+LG FFS+LLVTIV KVT HGKPWIPD
Sbjct: 483 GEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTLALGXFFSSLLVTIVGKVTEHGKPWIPD 542

Query: 578 NLNKGKLYNFYWLLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPTFH 631
           NLN+GKLY+FYWLLA+LSVLNL+VFL CAKWYVYK+KRLADEGIELED  PT H
Sbjct: 543 NLNEGKLYDFYWLLAVLSVLNLMVFLVCAKWYVYKEKRLADEGIELEDCGPTVH 591

BLAST of MC06g1318 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 744.2 bits (1920), Expect = 8.7e-215
Identity = 382/580 (65.86%), Postives = 463/580 (79.83%), Query Frame = 0

Query: 52  LPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSA 111
           L DAWD+ G+PA R++TGGW +AAMIL  EAVERLTTLGI VNLVTYLT TMHLGNAT+A
Sbjct: 12  LLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAA 71

Query: 112 NDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCS 171
           N VTNFLGTSFMLCLLGGF+ADTFLGRYLTI+IFAA+QATGV+ILT+STIIP LRPP+C+
Sbjct: 72  NTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCN 131

Query: 172 LGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFF 231
               +  C  A+G QL +LYLALYL ALGTGG+K+SVSGFGSDQFD+++ +ER KM+ FF
Sbjct: 132 -PTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFF 191

Query: 232 NWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGS 291
           N FFF I++GSL AVT+LVY+QD+VGR+WGYGICA AIVL L VFL+GT RYRFKKL+GS
Sbjct: 192 NRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGS 251

Query: 292 PLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAI 351
           P+TQ+A V +AAW+ R LELP+DP  L+D+DD +    + K KQKLP T+QFR LDKAAI
Sbjct: 252 PMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI 311

Query: 352 KDGE--IVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTM 411
           +D E  +  NV NKW +STLTD+EEVK ++RMLPIWAT ILFWTV+AQ+TTLSV+Q+ T+
Sbjct: 312 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 371

Query: 412 NRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVM 471
           +R IG SF+IP AS+ VF+V   L T  +YDR  + +  KL   P GL  LQRIG+GL  
Sbjct: 372 DRSIG-SFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFF 431

Query: 472 SILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDF 531
             +AM  AAL E+KRLR   A+G   K L   PL  + L PQ+L+VG GEA  Y GQLDF
Sbjct: 432 GSMAMAVAALVELKRLRTAHAHGPTVKTL---PLGFYLLIPQYLIVGIGEALIYTGQLDF 491

Query: 532 FLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPDNLNKGKLYNFYW 591
           FLRECPKGMK MSTGL LSTL+LGFFFS++LVTIV+K T    PWI D+LNKG+LYNFYW
Sbjct: 492 FLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYW 551

Query: 592 LLAILSVLNLVVFLFCAKWYVYKDKRLADEGIELEDSEPT 630
           L+A+L  LN ++FL  +KWYVYK+KRLA+ GIEL+D EP+
Sbjct: 552 LVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDD-EPS 585

BLAST of MC06g1318 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 594.7 bits (1532), Expect = 8.5e-170
Identity = 317/567 (55.91%), Postives = 412/567 (72.66%), Query Frame = 0

Query: 51  TLPDAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATS 110
           T+ DA DY G+PA +++TGGW+ AA+ILG E VERL+T+GIAVNLVTYL ETMHL ++TS
Sbjct: 8   TVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTS 67

Query: 111 ANDVTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKC 170
           AN VT+F+GTSF+LCLLGGF+AD+FLGR+ TI IF+ +QA G   L ++T +P LRPP C
Sbjct: 68  ANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC 127

Query: 171 SLGVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKF 230
             G     CIPA   Q+ ILY++LYL+ALGTGGLKSS+SGFGSDQFDD D +E+  M+ F
Sbjct: 128 HHG---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFF 187

Query: 231 FNWFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVG 290
           FN FFFFIS+G+L AVT+LVY+QD VGR W YGIC  ++ + +++FL GT+RYR+KK  G
Sbjct: 188 FNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQG 247

Query: 291 SPLTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAA 350
           SP+ QI  V  AA++KR +ELP     L+  +DN +         ++  T QF  LDKAA
Sbjct: 248 SPVVQIFQVIAAAFRKRKMELPQSIVYLY--EDNPEG-------IRIEHTDQFHLLDKAA 307

Query: 351 I-KDGEIVGN-----VVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVS 410
           I  +G+         + N W +S++T +EEVK+++R+LPIWATTI+FWT YAQM T SV 
Sbjct: 308 IVAEGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVE 367

Query: 411 QATTMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIG 470
           QA+TM R+IG SF+IPA SLTVFFVA+ L T+ +YDR I+P   K  K   G +SLQRI 
Sbjct: 368 QASTMRRNIG-SFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIA 427

Query: 471 VGLVMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYM 530
           +GLV+S   M  AAL E KRL   +++       + +P+SVF L PQF LVG+GEAF Y 
Sbjct: 428 IGLVLSTAGMAAAALVEQKRLSVAKSSS-----QKTLPISVFLLVPQFFLVGAGEAFIYT 487

Query: 531 GQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKP--WIPDNLNKG 590
           GQLDFF+ + PKGMKTMSTGLFL+TLSLGFF S+ LV+IV++VT+      W+ DN+N G
Sbjct: 488 GQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHG 547

Query: 591 KLYNFYWLLAILSVLNLVVFLFCAKWY 610
           +L  FYWLL ILS +N VV++ CA W+
Sbjct: 548 RLDYFYWLLVILSGINFVVYIICALWF 555

BLAST of MC06g1318 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 526.6 bits (1355), Expect = 2.8e-149
Identity = 290/565 (51.33%), Postives = 381/565 (67.43%), Query Frame = 0

Query: 54  DAWDYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSAND 113
           +A+DY G P  +++TGGW+ A +ILG+E  ER+  +GI++NLVTYL   +H+ +A SA  
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 114 VTNFLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKC-SL 173
           VTNF+GT  +L LLGGF+AD  LGRY  ++I A+V A GV +LT++T I S+RPP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 174 GVPNPPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFN 233
              +  CI ANG QLA+LY+ALY +ALG GG+KS+VSGFGSDQFD SD +E  +M  FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 234 WFFFFISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSP 293
            F+F IS+GSL AV  LVY+QDNVGR WGYGI A  +V+  IV L GT+RYRFKK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 294 LTQIATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAIK 353
            T I  V   AWKKR    P+ P LL   D+             +P T+  + LDKAAI 
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTT-----------VPHTEMLKCLDKAAIS 315

Query: 354 DGEIVGN-----VVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQAT 413
             E   +       + W +ST+T +EEVKLV++++PIWAT ILFWT+Y+QMTT +V QAT
Sbjct: 316 KNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQAT 375

Query: 414 TMNRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGL 473
            M+R +G SF +PA S + F + + L    + +R  VP+T +L K PQG+TSLQRIGVGL
Sbjct: 376 FMDRKLG-SFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGL 435

Query: 474 VMSILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQL 533
           V S+ AM  AA+ E  R  A   N        +  +S FWL PQ+ LVG+GEAF Y+GQL
Sbjct: 436 VFSMAAMAVAAVIENARREAAVNN--------DKKISAFWLVPQYFLVGAGEAFAYVGQL 495

Query: 534 DFFLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVTAHGKPWIPDNLNKGKLYNF 593
           +FF+RE P+ MK+MSTGLFLST+S+GFF S+LLV++V +VT   K W+  NLNK +L  F
Sbjct: 496 EFFIREAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVT--DKSWLRSNLNKARLNYF 555

Query: 594 YWLLAILSVLNLVVFLFCAKWYVYK 613
           YWLL +L  LN ++F+  A  + YK
Sbjct: 556 YWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of MC06g1318 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 498.4 bits (1282), Expect = 8.3e-141
Identity = 260/565 (46.02%), Postives = 386/565 (68.32%), Query Frame = 0

Query: 57  DYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSANDVTN 116
           D    PA++ +TG W A   ILG E  ERL   G+  NLV YL   ++ GNAT+AN+VTN
Sbjct: 14  DIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTN 73

Query: 117 FLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCSLGVPN 176
           + GT ++  L+G F+AD +LGRY TI+ F  +  +G+T+LT+S  +P L+P  C+    +
Sbjct: 74  WSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNADTCH 133

Query: 177 PPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFNWFFF 236
           P     N  Q A+ ++ALY++ALGTGG+K  VS FG+DQFD++D+ E+ K S FFNWF+F
Sbjct: 134 P-----NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYF 193

Query: 237 FISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSPLTQI 296
            I++G+L A T+LV+IQ NVG  WG+G+   A+V+ +  F  G+R YR ++  GSPLT+I
Sbjct: 194 SINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRI 253

Query: 297 ATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAA------ 356
             V +AA++K S+++P D  LLF   +  DD +  K  +KL  T   +F DKAA      
Sbjct: 254 FQVIVAAFRKISVKVPEDKSLLF---ETADDESNIKGSRKLVHTDNLKFFDKAAVESQSD 313

Query: 357 -IKDGEIVGNVVNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTM 416
            IKDGE     VN W + ++T +EE+K +I +LP+WAT I+F TVY+QM+T+ V Q  TM
Sbjct: 314 SIKDGE-----VNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 373

Query: 417 NRHIGKSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVM 476
           ++H+GK+F+IP+ASL++F   S LF  P+YD+FI+P+  K  +N +G T LQR+G+GLV+
Sbjct: 374 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 433

Query: 477 SILAMVTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDF 536
           SI AM+TA + E+ RL  V+ +   ++  +++ +S+FW  PQ+LL+G  E FT++GQL+F
Sbjct: 434 SIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 493

Query: 537 FLRECPKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVT-AHGKP-WIPDNLNKGKLYNF 596
           F  + P  M+++ + L L+T++LG + ST+LVT+V K+T  +GKP WIPDNLN+G L  F
Sbjct: 494 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYF 553

Query: 597 YWLLAILSVLNLVVFLFCAKWYVYK 613
           ++LLA LS LN +V+L+ +K Y YK
Sbjct: 554 FYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of MC06g1318 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.8e-128
Identity = 247/560 (44.11%), Postives = 369/560 (65.89%), Query Frame = 0

Query: 57  DYTGKPASRARTGGWVAAAMILGTEAVERLTTLGIAVNLVTYLTETMHLGNATSANDVTN 116
           D   KPA++ +TG W A   ILGTE  ERL   G++ NL+ YL + M++ N +++  V+N
Sbjct: 15  DIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSN 74

Query: 117 FLGTSFMLCLLGGFVADTFLGRYLTISIFAAVQATGVTILTISTIIPSLRPPKCSLGVPN 176
           + GT +   L+G F+AD +LGRY TI+ F  +   G+T+LTIS  +P L  P CS     
Sbjct: 75  WSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS----G 134

Query: 177 PPCIPANGKQLAILYLALYLLALGTGGLKSSVSGFGSDQFDDSDKEERGKMSKFFNWFFF 236
             C  A   Q AI ++ALYL+ALGTGG+K  VS FG+DQFDD+D++E+   S FFNWF+F
Sbjct: 135 ETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYF 194

Query: 237 FISIGSLGAVTILVYIQDNVGRQWGYGICACAIVLGLIVFLSGTRRYRFKKLVGSPLTQI 296
            I++G++ A ++LV+IQ NVG  WG G+   A+ + ++ F +G+  YR +K  GSPLT++
Sbjct: 195 VINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRM 254

Query: 297 ATVFIAAWKKRSLELPSDPFLLFDIDDNVDDGATKKKKQKLPRTKQFRFLDKAAIK-DGE 356
             V +A+ +K  +++P D  LL+   +N D  ++    +KL  TK   F DKAA++ + +
Sbjct: 255 LQVIVASCRKSKVKIPEDESLLY---ENQDAESSIIGSRKLEHTKILTFFDKAAVETESD 314

Query: 357 IVGNV-VNKWNMSTLTDIEEVKLVIRMLPIWATTILFWTVYAQMTTLSVSQATTMNRHIG 416
             G    + W + T+T +EE+K +IR+LPIWAT I+F +VY+QM T+ V Q  T+++H+G
Sbjct: 315 NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMG 374

Query: 417 KSFQIPAASLTVFFVASTLFTVPIYDRFIVPITSKLLKNPQGLTSLQRIGVGLVMSILAM 476
            +F+IP+ASL++F   S LF  P+YD+ IVP   K   + +G T LQRIG+GLV+SI +M
Sbjct: 375 PNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSM 434

Query: 477 VTAALTEIKRLRAVEANGLVEKPLEEVPLSVFWLAPQFLLVGSGEAFTYMGQLDFFLREC 536
           V+A + E+ RL  V+ + L  +  E +P+++FW  PQ+ LVG  E FT++GQL+FF  + 
Sbjct: 435 VSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 494

Query: 537 PKGMKTMSTGLFLSTLSLGFFFSTLLVTIVQKVT-AHGKP-WIPDNLNKGKLYNFYWLLA 596
           P  M+++ + L L+ ++ G + ST LVT+V KVT + G+P WI  NLN G L  F+WLLA
Sbjct: 495 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 554

Query: 597 ILSVLNLVVFLFCAKWYVYK 613
            LS LN +V+L+ AKWY YK
Sbjct: 555 GLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050851.2e-21365.86Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY41.2e-16855.91Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9LVE04.0e-14851.33Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q9M3901.2e-13946.02Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB83.9e-12744.11Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022156448.10.099.16protein NRT1/ PTR FAMILY 6.3-like isoform X1 [Momordica charantia][more]
XP_022156449.10.095.64protein NRT1/ PTR FAMILY 6.3-like isoform X2 [Momordica charantia][more]
XP_004147617.10.084.51protein NRT1/ PTR FAMILY 6.3 [Cucumis sativus] >KGN50229.1 hypothetical protein ... [more]
KAA0039694.10.084.01protein NRT1/ PTR FAMILY 6.3-like [Cucumis melo var. makuwa][more]
XP_008437115.10.083.84PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1DQN10.099.16protein NRT1/ PTR FAMILY 6.3-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1DTH40.095.64protein NRT1/ PTR FAMILY 6.3-like isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0KMV30.084.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G161290 PE=3 SV=1[more]
A0A5A7T8970.084.01Protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3ATS10.083.84LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 6.3-like OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT1G12110.18.7e-21565.86nitrate transporter 1.1 [more]
AT2G26690.18.5e-17055.91Major facilitator superfamily protein [more]
AT3G21670.12.8e-14951.33Major facilitator superfamily protein [more]
AT3G54140.18.3e-14146.02peptide transporter 1 [more]
AT5G01180.12.8e-12844.11peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 139..574
e-value: 3.8E-116
score: 388.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 50..623
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 43..619
e-value: 7.8E-200
score: 666.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 64..614
NoneNo IPR availablePANTHERPTHR11654:SF507PROTEIN NRT1/ PTR FAMILY 6.3coord: 50..623
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 128..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1318.1MC06g1318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity