MC06g1309 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1309
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNase H domain-containing protein
LocationMC06: 19966604 .. 19966939 (-)
RNA-Seq ExpressionMC06g1309
SyntenyMC06g1309
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAATGATGGTCTCGGAACAGGAGGAATCGGTTGGGTGATTCGAGATTGGGATGGTCAAACTGGGCGTGCAGGTTGTCTCTTTATAGCTGACAAACAAGAGGTCAAATTTTTGGAAACAATGGCTATTTGTGAAGGTTTTAAAGGTTTGAAGGAAGGAGGTTTTTACCCGAGTATAGCTGAATCGGACTCCCTCGAGGTCGTTCGTTTGCTGAATGGAGATGCTGAAGACCTCACTAAGATTCGATGGTTGATCAACGAGGCGAAAACTCGTGCTTCAGACGCCGGTGTAATTTCGATTCACCATATTGCGAGAGTTGGGAAAGACTTGGCTCAT

mRNA sequence

TGGAATGATGGTCTCGGAACAGGAGGAATCGGTTGGGTGATTCGAGATTGGGATGGTCAAACTGGGCGTGCAGGTTGTCTCTTTATAGCTGACAAACAAGAGGTCAAATTTTTGGAAACAATGGCTATTTGTGAAGGTTTTAAAGGTTTGAAGGAAGGAGGTTTTTACCCGAGTATAGCTGAATCGGACTCCCTCGAGGTCGTTCGTTTGCTGAATGGAGATGCTGAAGACCTCACTAAGATTCGATGGTTGATCAACGAGGCGAAAACTCGTGCTTCAGACGCCGGTGTAATTTCGATTCACCATATTGCGAGAGTTGGGAAAGACTTGGCTCAT

Coding sequence (CDS)

TGGAATGATGGTCTCGGAACAGGAGGAATCGGTTGGGTGATTCGAGATTGGGATGGTCAAACTGGGCGTGCAGGTTGTCTCTTTATAGCTGACAAACAAGAGGTCAAATTTTTGGAAACAATGGCTATTTGTGAAGGTTTTAAAGGTTTGAAGGAAGGAGGTTTTTACCCGAGTATAGCTGAATCGGACTCCCTCGAGGTCGTTCGTTTGCTGAATGGAGATGCTGAAGACCTCACTAAGATTCGATGGTTGATCAACGAGGCGAAAACTCGTGCTTCAGACGCCGGTGTAATTTCGATTCACCATATTGCGAGAGTTGGGAAAGACTTGGCTCAT

Protein sequence

WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIAESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH
Homology
BLAST of MC06g1309 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 93.2 bits (230), Expect = 1.05e-20
Identity = 43/112 (38.39%), Postives = 65/112 (58.04%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W     TGGIGW++RD  G+  +A C  I  ++ + +LE MAICEG + +++    P   
Sbjct: 95  WRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRPIHL 154

Query: 61  ESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           ESDSLE + LL+   +D T+I WL+ E      D  ++S+ HI+R    +AH
Sbjct: 155 ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAH 206

BLAST of MC06g1309 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 85.9 bits (211), Expect = 8.10e-18
Identity = 43/120 (35.83%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W     TGGIGW++RD  G+  +A C  I  ++ + +LE MAICEG + +++    P   
Sbjct: 95  WRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQ 154

Query: 61  E--------SDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           E        SDSLE + LL+   +D T+I WL+ E      D  ++S+ HI+R    +AH
Sbjct: 155 EHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAH 214

BLAST of MC06g1309 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 79.0 bits (193), Expect = 4.02e-15
Identity = 44/113 (38.94%), Postives = 55/113 (48.67%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKE-GGFYPSI 60
           W+D    GGIGW+IR WDG    AG  F+     VK LE  AI EG + L   G   P  
Sbjct: 87  WSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRPLH 146

Query: 61  AESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
            E+DS EV  LLN   EDLTK  W++ E         +++   + R     AH
Sbjct: 147 IETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAH 199

BLAST of MC06g1309 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 72.8 bits (177), Expect = 1.68e-13
Identity = 36/111 (32.43%), Postives = 60/111 (54.05%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W+   G GG+GW+IR+   +  +AG   I D +++ +LE MAI  G + +        I 
Sbjct: 21  WHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVVSSSSVSLII 80

Query: 61  ESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLA 111
           ES+SLE + L+ G  +++T+I WL+ + K     A +    H+ R G  +A
Sbjct: 81  ESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVA 131

BLAST of MC06g1309 vs. NCBI nr
Match: XP_038886170.1 (uncharacterized protein LOC120076417 [Benincasa hispida])

HSP 1 Score: 71.2 bits (173), Expect = 1.86e-12
Identity = 43/113 (38.05%), Postives = 59/113 (52.21%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           WN  +   G+GWV  D   +   AG  F+   Q+V  LE +AIC G + L        + 
Sbjct: 85  WNSKIDACGLGWVFLDHLYRVCMAGLKFVLRCQKVNILEAIAICFGLEILSSIDISNIMV 144

Query: 61  ESDSLE-VVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           ESD LE V+ LLN D  DL+++ +   EAK R  + GVIS  H+ R    LA+
Sbjct: 145 ESDCLEEVINLLNDDVVDLSEVSFCSEEAKDRGGNLGVISFSHVRRYRNVLAY 197

BLAST of MC06g1309 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.08e-21
Identity = 43/112 (38.39%), Postives = 65/112 (58.04%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W     TGGIGW++RD  G+  +A C  I  ++ + +LE MAICEG + +++    P   
Sbjct: 95  WRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRPIHL 154

Query: 61  ESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           ESDSLE + LL+   +D T+I WL+ E      D  ++S+ HI+R    +AH
Sbjct: 155 ESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAH 206

BLAST of MC06g1309 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.92e-18
Identity = 43/120 (35.83%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W     TGGIGW++RD  G+  +A C  I  ++ + +LE MAICEG + +++    P   
Sbjct: 95  WRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPIQQ 154

Query: 61  E--------SDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           E        SDSLE + LL+   +D T+I WL+ E      D  ++S+ HI+R    +AH
Sbjct: 155 EHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKVAH 214

BLAST of MC06g1309 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.95e-15
Identity = 44/113 (38.94%), Postives = 55/113 (48.67%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKE-GGFYPSI 60
           W+D    GGIGW+IR WDG    AG  F+     VK LE  AI EG + L   G   P  
Sbjct: 87  WSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRPLH 146

Query: 61  AESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
            E+DS EV  LLN   EDLTK  W++ E         +++   + R     AH
Sbjct: 147 IETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAH 199

BLAST of MC06g1309 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.15e-14
Identity = 36/111 (32.43%), Postives = 60/111 (54.05%), Query Frame = 0

Query: 1   WNDGLGTGGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIA 60
           W+   G GG+GW+IR+   +  +AG   I D +++ +LE MAI  G + +        I 
Sbjct: 21  WHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVVSSSSVSLII 80

Query: 61  ESDSLEVVRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLA 111
           ES+SLE + L+ G  +++T+I WL+ + K     A +    H+ R G  +A
Sbjct: 81  ESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVA 131

BLAST of MC06g1309 vs. ExPASy TrEMBL
Match: A0A5N5GSQ0 (RNase H domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_012800 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.05e-11
Identity = 37/105 (35.24%), Postives = 57/105 (54.29%), Query Frame = 0

Query: 8   GGIGWVIRDWDGQTGRAGCLFIADKQEVKFLETMAICEGFKGLKEGGFYPSIAESDSLEV 67
           GG+  V+RD+DG    A      D      +E +A   GF+   E GF+  I ESDSL++
Sbjct: 6   GGVEIVVRDFDGLCPVAPSRMFTDVFSPTLVEVLANRAGFELAVERGFHNVIVESDSLQL 65

Query: 68  VRLLNGDAEDLTKIRWLINEAKTRASDAGVISIHHIARVGKDLAH 112
           +  LNG + DL+ +R++I +AKT       +S  +I R   ++AH
Sbjct: 66  ITALNGSSMDLSPLRFIIEDAKTLLWSIAEVSATYIRRQANNVAH 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022143535.11.05e-2038.39uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_022156777.18.10e-1835.83uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022155262.14.02e-1538.94uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_022148737.11.68e-1332.43uncharacterized protein LOC111017329 [Momordica charantia][more]
XP_038886170.11.86e-1238.05uncharacterized protein LOC120076417 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1CP265.08e-2138.39uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DSV13.92e-1835.83uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DNV91.95e-1538.94uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1D5W18.15e-1432.43uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A5N5GSQ08.05e-1135.24RNase H domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=24484... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..112
e-value: 8.5E-8
score: 34.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 1..112
e-value: 2.5E-15
score: 56.4
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 1..112
e-value: 2.48522E-12
score: 56.9388
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 6..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1309.1MC06g1309.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity