MC06g1297 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1297
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationMC06: 19791235 .. 19792293 (-)
RNA-Seq ExpressionMC06g1297
SyntenyMC06g1297
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGAAAATTGCGCGGCAACTGTTTGATAAAAGTTCTGTGAAGGATGTGGTTGTGTGGACGGCGATGGTTGATGGGTATGGGAAGATGGGGGATGTTGAGAGTGCTAGGGTGTTGTTTGATGAAATGCCAGAGAAGAATGTAATTTCTTGGAGTGCTATGATGGCTGCCTATTCTCGGGTTAGTGACTTCAGAGAGGTGCTCTGTTTGTTTAGGCAAATGCAAGAGATGGATATTAAGCCCAATGAATCAGTTGTTGTCAGCGTTCTTACTGCCTGTGCTCATCTTGGTGCTATAACACAAGGGGTTTGGATGCACTCCTACGCCAAGCGGTACAGGTTGGAGTCTAACCCAATATTAGCCACTGCATTGGTTGACATGTATTCAAAATGTGGATACATCAAATCAGCTTTATCAGTTTTCGATGGTATTTCAAATAAAGATGCTGGGGCTTGGAATGCTATGATTTCAGGTGTTGCAATGAATGGAGATGTGAAGAAATCTCTTGAGTTATTTGATGAAATGGTCATCAAAGGAACTAATGCCACAGAAACTACATTTGTAGCTATCCTCACCGCTTGTACACATGCCAAGATGGTTGATAGGGGCCTTAAATTGTTTGACCAAATGTATACCGTTTATGGAGTTTGGCCTCAACTTGAGCATTTTGCTTGTGTGGTTGATCTTATGGCTAGAGCAGGTATGGTGGAGGATGCTGAGAGATTTATAGAGGAAAAAATGGGAGGCTTCAGTAAAGTGGACGCCAATGTGTGGGGAGCAGTACTTGGTGCTTGTAGAAAGTATGGGAATATTGAAGCTGGGAACAGAATTTGGAGAAAGCTTTCAGCTATGGGTATATCAGATTGTGGTACCCAAGTCCTCTCATACAACATTTATGCTGAAGCCGGGTGGGAAATGGCGGCAAAGGAGGTAAGAAAATTTTTTGCAGAGGCAAGAATGAAAAAGACACCAGGTTGCAGCGTGTTGGAGGTTGATGGTGCAGTTCAAGAGTTTCTTGCAGGCGATCTTTCTCATCCACAAGTGCAAGATATATGTGAAATG

mRNA sequence

TTGAAAATTGCGCGGCAACTGTTTGATAAAAGTTCTGTGAAGGATGTGGTTGTGTGGACGGCGATGGTTGATGGGTATGGGAAGATGGGGGATGTTGAGAGTGCTAGGGTGTTGTTTGATGAAATGCCAGAGAAGAATGTAATTTCTTGGAGTGCTATGATGGCTGCCTATTCTCGGGTTAGTGACTTCAGAGAGGTGCTCTGTTTGTTTAGGCAAATGCAAGAGATGGATATTAAGCCCAATGAATCAGTTGTTGTCAGCGTTCTTACTGCCTGTGCTCATCTTGGTGCTATAACACAAGGGGTTTGGATGCACTCCTACGCCAAGCGGTACAGGTTGGAGTCTAACCCAATATTAGCCACTGCATTGGTTGACATGTATTCAAAATGTGGATACATCAAATCAGCTTTATCAGTTTTCGATGGTATTTCAAATAAAGATGCTGGGGCTTGGAATGCTATGATTTCAGGTGTTGCAATGAATGGAGATGTGAAGAAATCTCTTGAGTTATTTGATGAAATGGTCATCAAAGGAACTAATGCCACAGAAACTACATTTGTAGCTATCCTCACCGCTTGTACACATGCCAAGATGGTTGATAGGGGCCTTAAATTGTTTGACCAAATGTATACCGTTTATGGAGTTTGGCCTCAACTTGAGCATTTTGCTTGTGTGGTTGATCTTATGGCTAGAGCAGGTATGGTGGAGGATGCTGAGAGATTTATAGAGGAAAAAATGGGAGGCTTCAGTAAAGTGGACGCCAATGTGTGGGGAGCAGTACTTGGTGCTTGTAGAAAGTATGGGAATATTGAAGCTGGGAACAGAATTTGGAGAAAGCTTTCAGCTATGGGTATATCAGATTGTGGTACCCAAGTCCTCTCATACAACATTTATGCTGAAGCCGGGTGGGAAATGGCGGCAAAGGAGGTAAGAAAATTTTTTGCAGAGGCAAGAATGAAAAAGACACCAGGTTGCAGCGTGTTGGAGGTTGATGGTGCAGTTCAAGAGTTTCTTGCAGGCGATCTTTCTCATCCACAAGTGCAAGATATATGTGAAATG

Coding sequence (CDS)

TTGAAAATTGCGCGGCAACTGTTTGATAAAAGTTCTGTGAAGGATGTGGTTGTGTGGACGGCGATGGTTGATGGGTATGGGAAGATGGGGGATGTTGAGAGTGCTAGGGTGTTGTTTGATGAAATGCCAGAGAAGAATGTAATTTCTTGGAGTGCTATGATGGCTGCCTATTCTCGGGTTAGTGACTTCAGAGAGGTGCTCTGTTTGTTTAGGCAAATGCAAGAGATGGATATTAAGCCCAATGAATCAGTTGTTGTCAGCGTTCTTACTGCCTGTGCTCATCTTGGTGCTATAACACAAGGGGTTTGGATGCACTCCTACGCCAAGCGGTACAGGTTGGAGTCTAACCCAATATTAGCCACTGCATTGGTTGACATGTATTCAAAATGTGGATACATCAAATCAGCTTTATCAGTTTTCGATGGTATTTCAAATAAAGATGCTGGGGCTTGGAATGCTATGATTTCAGGTGTTGCAATGAATGGAGATGTGAAGAAATCTCTTGAGTTATTTGATGAAATGGTCATCAAAGGAACTAATGCCACAGAAACTACATTTGTAGCTATCCTCACCGCTTGTACACATGCCAAGATGGTTGATAGGGGCCTTAAATTGTTTGACCAAATGTATACCGTTTATGGAGTTTGGCCTCAACTTGAGCATTTTGCTTGTGTGGTTGATCTTATGGCTAGAGCAGGTATGGTGGAGGATGCTGAGAGATTTATAGAGGAAAAAATGGGAGGCTTCAGTAAAGTGGACGCCAATGTGTGGGGAGCAGTACTTGGTGCTTGTAGAAAGTATGGGAATATTGAAGCTGGGAACAGAATTTGGAGAAAGCTTTCAGCTATGGGTATATCAGATTGTGGTACCCAAGTCCTCTCATACAACATTTATGCTGAAGCCGGGTGGGAAATGGCGGCAAAGGAGGTAAGAAAATTTTTTGCAGAGGCAAGAATGAAAAAGACACCAGGTTGCAGCGTGTTGGAGGTTGATGGTGCAGTTCAAGAGTTTCTTGCAGGCGATCTTTCTCATCCACAAGTGCAAGATATATGTGAAATG

Protein sequence

LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Homology
BLAST of MC06g1297 vs. ExPASy Swiss-Prot
Match: Q9SJZ3 (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.3e-76
Identity = 139/347 (40.06%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           AR++FD    + +V WT M+ GY + G ++ +R LFD+M EK+V+ W+AM+    +    
Sbjct: 311 ARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRG 370

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
           ++ L LF++MQ  + KP+E  ++  L+AC+ LGA+  G+W+H Y ++Y L  N  L T+L
Sbjct: 371 QDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSL 430

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDMY+KCG I  ALSVF GI  +++  + A+I G+A++GD   ++  F+EM+  G    E
Sbjct: 431 VDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAGIAPDE 490

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TF+ +L+AC H  M+  G   F QM + + + PQL+H++ +VDL+ RAG++E+A+R +E
Sbjct: 491 ITFIGLLSACCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLME 550

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
                  + DA VWGA+L  CR +GN+E G +  +KL  +  SD G  VL   +Y EA  
Sbjct: 551 SMP---MEADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANM 610

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDI 351
              AK  R+   E  ++K PGCS +EV+G V EF+  D S P+ + I
Sbjct: 611 WEDAKRARRMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKI 654

BLAST of MC06g1297 vs. ExPASy Swiss-Prot
Match: Q9C866 (Pentatricopeptide repeat-containing protein At1g31430 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E55 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.3e-75
Identity = 144/352 (40.91%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           AR +FD    K+V  WT+MV GY   G ++ ARVLF+  P K+V+ W+AMM  Y + + F
Sbjct: 201 ARAVFDSMRDKNVKCWTSMVFGYVSTGRIDEARVLFERSPVKDVVLWTAMMNGYVQFNRF 260

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
            E L LFR MQ   I+P+  V+VS+LT CA  GA+ QG W+H Y    R+  + ++ TAL
Sbjct: 261 DEALELFRCMQTAGIRPDNFVLVSLLTGCAQTGALEQGKWIHGYINENRVTVDKVVGTAL 320

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDMY+KCG I++AL VF  I  +D  +W ++I G+AMNG   ++L+L+ EM   G     
Sbjct: 321 VDMYAKCGCIETALEVFYEIKERDTASWTSLIYGLAMNGMSGRALDLYYEMENVGVRLDA 380

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TFVA+LTAC H   V  G K+F  M   + V P+ EH +C++DL+ RAG++++AE  I+
Sbjct: 381 ITFVAVLTACNHGGFVAEGRKIFHSMTERHNVQPKSEHCSCLIDLLCRAGLLDEAEELID 440

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
           +  G   +    V+ ++L A R YGN++   R+  KL  + +SD     L  ++YA A  
Sbjct: 441 KMRGESDETLVPVYCSLLSAARNYGNVKIAERVAEKLEKVEVSDSSAHTLLASVYASANR 500

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGD--LSHPQVQDICEM 354
                 VR+   +  ++K PGCS +E+DG   EF+ GD  LSHP++ +I  M
Sbjct: 501 WEDVTNVRRKMKDLGIRKFPGCSSIEIDGVGHEFIVGDDLLSHPKMDEINSM 552

BLAST of MC06g1297 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.1e-74
Identity = 142/350 (40.57%), Postives = 211/350 (60.29%), Query Frame = 0

Query: 2   KIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVS 61
           K+A  LFD+    D V W +++ GY K G ++ A  LF +M EKN ISW+ M++ Y +  
Sbjct: 167 KLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQAD 226

Query: 62  DFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILAT 121
             +E L LF +MQ  D++P+   + + L+ACA LGA+ QG W+HSY  + R+  + +L  
Sbjct: 227 MNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGC 286

Query: 122 ALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNA 181
            L+DMY+KCG ++ AL VF  I  K   AW A+ISG A +G  ++++  F EM   G   
Sbjct: 287 VLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKP 346

Query: 182 TETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERF 241
              TF A+LTAC++  +V+ G  +F  M   Y + P +EH+ C+VDL+ RAG++++A+RF
Sbjct: 347 NVITFTAVLTACSYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRF 406

Query: 242 IEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYA-E 301
           I+E      K +A +WGA+L ACR + NIE G  I   L A+     G  V   NI+A +
Sbjct: 407 IQEMP---LKPNAVIWGALLKACRIHKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMD 466

Query: 302 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDI 351
             W+ AA E R+   E  + K PGCS + ++G   EFLAGD SHP+++ I
Sbjct: 467 KKWDKAA-ETRRLMKEQGVAKVPGCSTISLEGTTHEFLAGDRSHPEIEKI 512

BLAST of MC06g1297 vs. ExPASy Swiss-Prot
Match: Q9FI80 (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 136/367 (37.06%), Postives = 229/367 (62.40%), Query Frame = 0

Query: 1   LKIARQLFDKSSVK--------------DVVVWTAMVDGYGKMGDVESARVLFDEMPEKN 60
           +K AR LF K+ ++              ++V+W  M+DGY ++GD ++AR+LFD+M +++
Sbjct: 179 MKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQRS 238

Query: 61  VISWSAMMAAYSRVSDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHS 120
           V+SW+ M++ YS    F++ + +FR+M++ DI+PN   +VSVL A + LG++  G W+H 
Sbjct: 239 VVSWNTMISGYSLNGFFKDAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHL 298

Query: 121 YAKRYRLESNPILATALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKK 180
           YA+   +  + +L +AL+DMYSKCG I+ A+ VF+ +  ++   W+AMI+G A++G    
Sbjct: 299 YAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGD 358

Query: 181 SLELFDEMVIKGTNATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVV 240
           +++ F +M   G   ++  ++ +LTAC+H  +V+ G + F QM +V G+ P++EH+ C+V
Sbjct: 359 AIDCFCKMRQAGVRPSDVAYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMV 418

Query: 241 DLMARAGMVEDAERFIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGIS 300
           DL+ R+G++++AE FI   +    K D  +W A+LGACR  GN+E G R+   L  M   
Sbjct: 419 DLLGRSGLLDEAEEFI---LNMPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPH 478

Query: 301 DCGTQVLSYNIYAEAGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQ 354
           D G  V   N+YA  G      E+R    E  ++K PGCS++++DG + EF+  D SHP+
Sbjct: 479 DSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPK 538

BLAST of MC06g1297 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.9e-70
Identity = 135/348 (38.79%), Postives = 217/348 (62.36%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           A ++F +   +DVV WT+MV GY K G VE+AR +FDEMP +N+ +WS M+  Y++ + F
Sbjct: 171 AGRIFGQMGFRDVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCF 230

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
            + + LF  M+   +  NE+V+VSV+++CAHLGA+  G   + Y  +  +  N IL TAL
Sbjct: 231 EKAIDLFEFMKREGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTAL 290

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDM+ +CG I+ A+ VF+G+   D+ +W+++I G+A++G   K++  F +M+  G    +
Sbjct: 291 VDMFWRCGDIEKAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRD 350

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TF A+L+AC+H  +V++GL++++ M   +G+ P+LEH+ C+VD++ RAG + +AE FI 
Sbjct: 351 VTFTAVLSACSHGGLVEKGLEIYENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFI- 410

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
             +    K +A + GA+LGAC+ Y N E   R+   L  +     G  VL  NIYA AG 
Sbjct: 411 --LKMHVKPNAPILGALLGACKIYKNTEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQ 470

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAG-DLSHPQVQDI 351
               + +R    E  +KK PG S++E+DG + +F  G D  HP++  I
Sbjct: 471 WDKIESLRDMMKEKLVKKPPGWSLIEIDGKINKFTMGDDQKHPEMGKI 515

BLAST of MC06g1297 vs. NCBI nr
Match: XP_022154784.1 (pentatricopeptide repeat-containing protein At5g66520-like [Momordica charantia])

HSP 1 Score: 710 bits (1832), Expect = 3.54e-255
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV
Sbjct: 162 LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA
Sbjct: 222 SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN
Sbjct: 282 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER
Sbjct: 342 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE
Sbjct: 402 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. NCBI nr
Match: XP_038875707.1 (pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida] >XP_038875708.1 pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida] >XP_038875709.1 pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida] >XP_038875711.1 pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida])

HSP 1 Score: 639 bits (1647), Expect = 5.01e-227
Identity = 310/353 (87.82%), Postives = 335/353 (94.90%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LKIAR LFDKSSVKDVVVWTAMVDGYGK+GDVE+ARVLFDEMPE+NVISWSAMMAAYSRV
Sbjct: 162 LKIARVLFDKSSVKDVVVWTAMVDGYGKIGDVENARVLFDEMPERNVISWSAMMAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVL LFRQMQE DI+PNESV+VSVLTACAHLGAITQG+WMHSYAKRY L+SNPILA
Sbjct: 222 SDFREVLSLFRQMQEKDIRPNESVIVSVLTACAHLGAITQGLWMHSYAKRYGLDSNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GISNKDAGAWNAMISGVAMNGDV KSLELF++M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGISNKDAGAWNAMISGVAMNGDVMKSLELFNKMIVNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFV IL ACTHAKMV+RGLKLF+QMY VYGV PQ EH ACVVDLMARAGMVEDAE+
Sbjct: 342 ATETTFVVILAACTHAKMVERGLKLFNQMYPVYGVQPQFEHHACVVDLMARAGMVEDAEK 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           F+EEKMGGF  VDANVWGA+LGACRKYGNIE GNRIW+KLSAMG+SDCGTQVLSYNIY+E
Sbjct: 402 FVEEKMGGFGNVDANVWGAILGACRKYGNIEIGNRIWKKLSAMGVSDCGTQVLSYNIYSE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK F+EA++KKTPGCSVLEVDG VQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKKFSEAKLKKTPGCSVLEVDGTVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. NCBI nr
Match: XP_022922110.1 (pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita moschata] >XP_022922111.1 pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita moschata] >XP_022922112.1 pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita moschata] >XP_022922113.1 pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita moschata])

HSP 1 Score: 636 bits (1641), Expect = 4.10e-226
Identity = 310/353 (87.82%), Postives = 331/353 (93.77%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSSVKDVVVWTAMVDGYGK+GDVESAR LFDEMP +NVISWSAM+AAYSRV
Sbjct: 162 LKVARVLFDKSSVKDVVVWTAMVDGYGKVGDVESARELFDEMPVRNVISWSAMIAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQE DIKPNES+ VSVLTACAHLGAITQG+WMHSYAKR+R ESNPILA
Sbjct: 222 SDFREVLCLFRQMQEKDIKPNESITVSVLTACAHLGAITQGLWMHSYAKRHRWESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GI NKDAGAWNAMISGVAMNGDV KSLELFD+M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGIPNKDAGAWNAMISGVAMNGDVTKSLELFDKMILNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVA+L ACTHAKMVD GLK+FDQMYTVYGV PQ EH+ACVVDLMARAGMVEDAE 
Sbjct: 342 ATETTFVAVLAACTHAKMVDTGLKIFDQMYTVYGVRPQFEHYACVVDLMARAGMVEDAEE 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIE+KMGGFS VDANVWGA+LGACRKYGNIE GNRIWRKL AMG++DCGTQVLSYNIYAE
Sbjct: 402 FIEKKMGGFSNVDANVWGAMLGACRKYGNIEVGNRIWRKLLAMGVTDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK  +E R KKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKNLSETRSKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. NCBI nr
Match: KAG6579406.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 636 bits (1640), Expect = 5.82e-226
Identity = 310/353 (87.82%), Postives = 331/353 (93.77%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSSVKDVVVWTAMVDGYGK+GDVESAR LFDEMP +NVISWSAM+AAYSRV
Sbjct: 162 LKVARVLFDKSSVKDVVVWTAMVDGYGKVGDVESARELFDEMPVRNVISWSAMIAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQE DIKPNES+ VSVLTACAHLGAITQG+WMHSYAKR+R ESNPILA
Sbjct: 222 SDFREVLCLFRQMQERDIKPNESITVSVLTACAHLGAITQGLWMHSYAKRHRWESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GI NKDAGAWNAMISGVAMNGDV KSLELFD+M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGILNKDAGAWNAMISGVAMNGDVTKSLELFDKMILNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVA+L ACTHAKMVD GLK+FDQMYTVYGV PQ EH+ACVVDLMARAGMVEDAE 
Sbjct: 342 ATETTFVAVLAACTHAKMVDTGLKIFDQMYTVYGVRPQFEHYACVVDLMARAGMVEDAEE 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIE+KMGGFS VDANVWGA+LGACRKYGNIE GNRIWRKL AMG++DCGTQVLSYNIYAE
Sbjct: 402 FIEKKMGGFSNVDANVWGAMLGACRKYGNIEVGNRIWRKLLAMGVTDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK  +E R KKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKNLSETRSKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. NCBI nr
Match: XP_023549650.1 (pentatricopeptide repeat-containing protein At5g06540-like [Cucurbita pepo subsp. pepo] >XP_023549651.1 pentatricopeptide repeat-containing protein At5g06540-like [Cucurbita pepo subsp. pepo] >XP_023549652.1 pentatricopeptide repeat-containing protein At5g06540-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 635 bits (1639), Expect = 8.25e-226
Identity = 311/353 (88.10%), Postives = 331/353 (93.77%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSSVKDVVVWTAMVDGYGK+GDVESAR LFDEMP +NVISWSAM+AAYSRV
Sbjct: 162 LKVARVLFDKSSVKDVVVWTAMVDGYGKVGDVESARELFDEMPVRNVISWSAMIAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQE DIKPNES+ VSVLTACAHLGAITQG+WMHSYAKR+R ESN ILA
Sbjct: 222 SDFREVLCLFRQMQEKDIKPNESITVSVLTACAHLGAITQGLWMHSYAKRHRWESNLILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GISNKDAGAWNAMISGVAMNGDV KSLELFD+M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGISNKDAGAWNAMISGVAMNGDVTKSLELFDKMILNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVA+L ACTHAKMVD GLKLFDQMYTVYGV PQ EH+ACVVDLMARAGMVEDAE 
Sbjct: 342 ATETTFVAVLAACTHAKMVDTGLKLFDQMYTVYGVQPQFEHYACVVDLMARAGMVEDAEE 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIE+KMGGFS VDANVWGA+LGACRKYGNIE GNRIWRKL AMG++DCGTQVLSYNIYAE
Sbjct: 402 FIEKKMGGFSNVDANVWGAMLGACRKYGNIEVGNRIWRKLLAMGVTDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK  +E R KKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKNLSETRSKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. ExPASy TrEMBL
Match: A0A6J1DN73 (pentatricopeptide repeat-containing protein At5g66520-like OS=Momordica charantia OX=3673 GN=LOC111021956 PE=4 SV=1)

HSP 1 Score: 710 bits (1832), Expect = 1.71e-255
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV
Sbjct: 162 LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA
Sbjct: 222 SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN
Sbjct: 282 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER
Sbjct: 342 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE
Sbjct: 402 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. ExPASy TrEMBL
Match: A0A6J1E2B1 (pentatricopeptide repeat-containing protein At5g66520-like OS=Cucurbita moschata OX=3662 GN=LOC111430180 PE=4 SV=1)

HSP 1 Score: 636 bits (1641), Expect = 1.98e-226
Identity = 310/353 (87.82%), Postives = 331/353 (93.77%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSSVKDVVVWTAMVDGYGK+GDVESAR LFDEMP +NVISWSAM+AAYSRV
Sbjct: 162 LKVARVLFDKSSVKDVVVWTAMVDGYGKVGDVESARELFDEMPVRNVISWSAMIAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQE DIKPNES+ VSVLTACAHLGAITQG+WMHSYAKR+R ESNPILA
Sbjct: 222 SDFREVLCLFRQMQEKDIKPNESITVSVLTACAHLGAITQGLWMHSYAKRHRWESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GI NKDAGAWNAMISGVAMNGDV KSLELFD+M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGIPNKDAGAWNAMISGVAMNGDVTKSLELFDKMILNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVA+L ACTHAKMVD GLK+FDQMYTVYGV PQ EH+ACVVDLMARAGMVEDAE 
Sbjct: 342 ATETTFVAVLAACTHAKMVDTGLKIFDQMYTVYGVRPQFEHYACVVDLMARAGMVEDAEE 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIE+KMGGFS VDANVWGA+LGACRKYGNIE GNRIWRKL AMG++DCGTQVLSYNIYAE
Sbjct: 402 FIEKKMGGFSNVDANVWGAMLGACRKYGNIEVGNRIWRKLLAMGVTDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK  +E R KKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKNLSETRSKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. ExPASy TrEMBL
Match: A0A6J1HYB2 (pentatricopeptide repeat-containing protein At5g48910-like OS=Cucurbita maxima OX=3661 GN=LOC111469133 PE=4 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 7.64e-224
Identity = 308/353 (87.25%), Postives = 329/353 (93.20%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK AR LFDKSSVKDVVVWTAMVDGYGK+GDVESAR LFDEMP +NVISWSAM+AAYSRV
Sbjct: 162 LKTARVLFDKSSVKDVVVWTAMVDGYGKVGDVESARELFDEMPVRNVISWSAMIAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFRQMQE DIKPNES+ VSVLTACAHLGAITQG+WMHSYAKR+R ESNPILA
Sbjct: 222 SDFREVLCLFRQMQEKDIKPNESITVSVLTACAHLGAITQGLWMHSYAKRHRWESNPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL+VF+GI NKDAGAWNAMISGVAMNGDV KSL+LFD+M++ GT 
Sbjct: 282 TALVDMYSKCGYIESALAVFEGIPNKDAGAWNAMISGVAMNGDVTKSLKLFDKMILNGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATETTFVA+L ACTHAKMVD GLKLFDQMYTVYGV PQ EH+ACVVDLMARAGMVEDAE 
Sbjct: 342 ATETTFVAVLAACTHAKMVDTGLKLFDQMYTVYGVRPQFEHYACVVDLMARAGMVEDAEE 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           FIE+KM GFS VDANVWGA+LGACRKYGNIE GNRIW+KL AMG++DCGTQVLSYNIYAE
Sbjct: 402 FIEKKMEGFSNVDANVWGAMLGACRKYGNIEVGNRIWKKLLAMGVTDCGTQVLSYNIYAE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AGWEMAAKEVRK  +E R KKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM
Sbjct: 462 AGWEMAAKEVRKNLSETRSKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 514

BLAST of MC06g1297 vs. ExPASy TrEMBL
Match: A0A5A7T9N4 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00550 PE=4 SV=1)

HSP 1 Score: 605 bits (1559), Expect = 5.26e-214
Identity = 295/353 (83.57%), Postives = 324/353 (91.78%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSS KDVVVWT MVDGYGK+GD+ESARVLFDEMPE+NVISWSAMMAAYSRV
Sbjct: 162 LKVARVLFDKSSAKDVVVWTVMVDGYGKVGDIESARVLFDEMPERNVISWSAMMAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFR+MQ+ DIKPN+SV+VSVLTACAHLGAITQG+WMHSYAKRY L++NPILA
Sbjct: 222 SDFREVLCLFREMQKKDIKPNDSVIVSVLTACAHLGAITQGLWMHSYAKRYGLDANPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL VF+GISNKDAGAWNAMISGVAM GDV KSLELFD+M+  GT 
Sbjct: 282 TALVDMYSKCGYIESALEVFEGISNKDAGAWNAMISGVAMTGDVMKSLELFDKMIASGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATE TFV+IL ACTHAKMV+RGL+LFDQMY VY V PQ EH+ACVVDLMARAGMVEDAE+
Sbjct: 342 ATEATFVSILAACTHAKMVERGLELFDQMYPVYWVQPQFEHYACVVDLMARAGMVEDAEK 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           F+EEKMGGFS VDANVWGA+L ACRKY NIE G+RIW KLSA+G+ D GTQVLSYNI++E
Sbjct: 402 FVEEKMGGFSNVDANVWGAILSACRKYRNIEIGDRIWTKLSALGVLDGGTQVLSYNIFSE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AG EMAAKEVRK F+EAR KK PGCSV+EVDGAVQEFLAGDLSHPQVQ ICEM
Sbjct: 462 AGREMAAKEVRKKFSEARSKKIPGCSVIEVDGAVQEFLAGDLSHPQVQHICEM 514

BLAST of MC06g1297 vs. ExPASy TrEMBL
Match: A0A1S3ATN3 (pentatricopeptide repeat-containing protein At5g66520-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482605 PE=4 SV=1)

HSP 1 Score: 605 bits (1559), Expect = 5.26e-214
Identity = 295/353 (83.57%), Postives = 324/353 (91.78%), Query Frame = 0

Query: 1   LKIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRV 60
           LK+AR LFDKSS KDVVVWT MVDGYGK+GD+ESARVLFDEMPE+NVISWSAMMAAYSRV
Sbjct: 162 LKVARVLFDKSSAKDVVVWTVMVDGYGKVGDIESARVLFDEMPERNVISWSAMMAAYSRV 221

Query: 61  SDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILA 120
           SDFREVLCLFR+MQ+ DIKPN+SV+VSVLTACAHLGAITQG+WMHSYAKRY L++NPILA
Sbjct: 222 SDFREVLCLFREMQKKDIKPNDSVIVSVLTACAHLGAITQGLWMHSYAKRYGLDANPILA 281

Query: 121 TALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTN 180
           TALVDMYSKCGYI+SAL VF+GISNKDAGAWNAMISGVAM GDV KSLELFD+M+  GT 
Sbjct: 282 TALVDMYSKCGYIESALEVFEGISNKDAGAWNAMISGVAMTGDVMKSLELFDKMIASGTQ 341

Query: 181 ATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAER 240
           ATE TFV+IL ACTHAKMV+RGL+LFDQMY VY V PQ EH+ACVVDLMARAGMVEDAE+
Sbjct: 342 ATEATFVSILAACTHAKMVERGLELFDQMYPVYWVQPQFEHYACVVDLMARAGMVEDAEK 401

Query: 241 FIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAE 300
           F+EEKMGGFS VDANVWGA+L ACRKY NIE G+RIW KLSA+G+ D GTQVLSYNI++E
Sbjct: 402 FVEEKMGGFSNVDANVWGAILSACRKYRNIEIGDRIWTKLSALGVLDGGTQVLSYNIFSE 461

Query: 301 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDICEM 353
           AG EMAAKEVRK F+EAR KK PGCSV+EVDGAVQEFLAGDLSHPQVQ ICEM
Sbjct: 462 AGREMAAKEVRKKFSEARSKKIPGCSVIEVDGAVQEFLAGDLSHPQVQHICEM 514

BLAST of MC06g1297 vs. TAIR 10
Match: AT2G22410.1 (SLOW GROWTH 1 )

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-77
Identity = 139/347 (40.06%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           AR++FD    + +V WT M+ GY + G ++ +R LFD+M EK+V+ W+AM+    +    
Sbjct: 311 ARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRG 370

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
           ++ L LF++MQ  + KP+E  ++  L+AC+ LGA+  G+W+H Y ++Y L  N  L T+L
Sbjct: 371 QDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLSLNVALGTSL 430

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDMY+KCG I  ALSVF GI  +++  + A+I G+A++GD   ++  F+EM+  G    E
Sbjct: 431 VDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEMIDAGIAPDE 490

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TF+ +L+AC H  M+  G   F QM + + + PQL+H++ +VDL+ RAG++E+A+R +E
Sbjct: 491 ITFIGLLSACCHGGMIQTGRDYFSQMKSRFNLNPQLKHYSIMVDLLGRAGLLEEADRLME 550

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
                  + DA VWGA+L  CR +GN+E G +  +KL  +  SD G  VL   +Y EA  
Sbjct: 551 SMP---MEADAAVWGALLFGCRMHGNVELGEKAAKKLLELDPSDSGIYVLLDGMYGEANM 610

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDI 351
              AK  R+   E  ++K PGCS +EV+G V EF+  D S P+ + I
Sbjct: 611 WEDAKRARRMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKI 654

BLAST of MC06g1297 vs. TAIR 10
Match: AT1G31430.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-76
Identity = 144/352 (40.91%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           AR +FD    K+V  WT+MV GY   G ++ ARVLF+  P K+V+ W+AMM  Y + + F
Sbjct: 201 ARAVFDSMRDKNVKCWTSMVFGYVSTGRIDEARVLFERSPVKDVVLWTAMMNGYVQFNRF 260

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
            E L LFR MQ   I+P+  V+VS+LT CA  GA+ QG W+H Y    R+  + ++ TAL
Sbjct: 261 DEALELFRCMQTAGIRPDNFVLVSLLTGCAQTGALEQGKWIHGYINENRVTVDKVVGTAL 320

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDMY+KCG I++AL VF  I  +D  +W ++I G+AMNG   ++L+L+ EM   G     
Sbjct: 321 VDMYAKCGCIETALEVFYEIKERDTASWTSLIYGLAMNGMSGRALDLYYEMENVGVRLDA 380

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TFVA+LTAC H   V  G K+F  M   + V P+ EH +C++DL+ RAG++++AE  I+
Sbjct: 381 ITFVAVLTACNHGGFVAEGRKIFHSMTERHNVQPKSEHCSCLIDLLCRAGLLDEAEELID 440

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
           +  G   +    V+ ++L A R YGN++   R+  KL  + +SD     L  ++YA A  
Sbjct: 441 KMRGESDETLVPVYCSLLSAARNYGNVKIAERVAEKLEKVEVSDSSAHTLLASVYASANR 500

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGD--LSHPQVQDICEM 354
                 VR+   +  ++K PGCS +E+DG   EF+ GD  LSHP++ +I  M
Sbjct: 501 WEDVTNVRRKMKDLGIRKFPGCSSIEIDGVGHEFIVGDDLLSHPKMDEINSM 552

BLAST of MC06g1297 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.8e-75
Identity = 142/350 (40.57%), Postives = 211/350 (60.29%), Query Frame = 0

Query: 2   KIARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVS 61
           K+A  LFD+    D V W +++ GY K G ++ A  LF +M EKN ISW+ M++ Y +  
Sbjct: 167 KLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQAD 226

Query: 62  DFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILAT 121
             +E L LF +MQ  D++P+   + + L+ACA LGA+ QG W+HSY  + R+  + +L  
Sbjct: 227 MNKEALQLFHEMQNSDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGC 286

Query: 122 ALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNA 181
            L+DMY+KCG ++ AL VF  I  K   AW A+ISG A +G  ++++  F EM   G   
Sbjct: 287 VLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKP 346

Query: 182 TETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERF 241
              TF A+LTAC++  +V+ G  +F  M   Y + P +EH+ C+VDL+ RAG++++A+RF
Sbjct: 347 NVITFTAVLTACSYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRF 406

Query: 242 IEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYA-E 301
           I+E      K +A +WGA+L ACR + NIE G  I   L A+     G  V   NI+A +
Sbjct: 407 IQEMP---LKPNAVIWGALLKACRIHKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMD 466

Query: 302 AGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQVQDI 351
             W+ AA E R+   E  + K PGCS + ++G   EFLAGD SHP+++ I
Sbjct: 467 KKWDKAA-ETRRLMKEQGVAKVPGCSTISLEGTTHEFLAGDRSHPEIEKI 512

BLAST of MC06g1297 vs. TAIR 10
Match: AT5G48910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 9.8e-75
Identity = 136/367 (37.06%), Postives = 229/367 (62.40%), Query Frame = 0

Query: 1   LKIARQLFDKSSVK--------------DVVVWTAMVDGYGKMGDVESARVLFDEMPEKN 60
           +K AR LF K+ ++              ++V+W  M+DGY ++GD ++AR+LFD+M +++
Sbjct: 179 MKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQRS 238

Query: 61  VISWSAMMAAYSRVSDFREVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHS 120
           V+SW+ M++ YS    F++ + +FR+M++ DI+PN   +VSVL A + LG++  G W+H 
Sbjct: 239 VVSWNTMISGYSLNGFFKDAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHL 298

Query: 121 YAKRYRLESNPILATALVDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKK 180
           YA+   +  + +L +AL+DMYSKCG I+ A+ VF+ +  ++   W+AMI+G A++G    
Sbjct: 299 YAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGD 358

Query: 181 SLELFDEMVIKGTNATETTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVV 240
           +++ F +M   G   ++  ++ +LTAC+H  +V+ G + F QM +V G+ P++EH+ C+V
Sbjct: 359 AIDCFCKMRQAGVRPSDVAYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMV 418

Query: 241 DLMARAGMVEDAERFIEEKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGIS 300
           DL+ R+G++++AE FI   +    K D  +W A+LGACR  GN+E G R+   L  M   
Sbjct: 419 DLLGRSGLLDEAEEFI---LNMPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPH 478

Query: 301 DCGTQVLSYNIYAEAGWEMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAGDLSHPQ 354
           D G  V   N+YA  G      E+R    E  ++K PGCS++++DG + EF+  D SHP+
Sbjct: 479 DSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPK 538

BLAST of MC06g1297 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 135/348 (38.79%), Postives = 217/348 (62.36%), Query Frame = 0

Query: 4   ARQLFDKSSVKDVVVWTAMVDGYGKMGDVESARVLFDEMPEKNVISWSAMMAAYSRVSDF 63
           A ++F +   +DVV WT+MV GY K G VE+AR +FDEMP +N+ +WS M+  Y++ + F
Sbjct: 171 AGRIFGQMGFRDVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCF 230

Query: 64  REVLCLFRQMQEMDIKPNESVVVSVLTACAHLGAITQGVWMHSYAKRYRLESNPILATAL 123
            + + LF  M+   +  NE+V+VSV+++CAHLGA+  G   + Y  +  +  N IL TAL
Sbjct: 231 EKAIDLFEFMKREGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTAL 290

Query: 124 VDMYSKCGYIKSALSVFDGISNKDAGAWNAMISGVAMNGDVKKSLELFDEMVIKGTNATE 183
           VDM+ +CG I+ A+ VF+G+   D+ +W+++I G+A++G   K++  F +M+  G    +
Sbjct: 291 VDMFWRCGDIEKAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRD 350

Query: 184 TTFVAILTACTHAKMVDRGLKLFDQMYTVYGVWPQLEHFACVVDLMARAGMVEDAERFIE 243
            TF A+L+AC+H  +V++GL++++ M   +G+ P+LEH+ C+VD++ RAG + +AE FI 
Sbjct: 351 VTFTAVLSACSHGGLVEKGLEIYENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFI- 410

Query: 244 EKMGGFSKVDANVWGAVLGACRKYGNIEAGNRIWRKLSAMGISDCGTQVLSYNIYAEAGW 303
             +    K +A + GA+LGAC+ Y N E   R+   L  +     G  VL  NIYA AG 
Sbjct: 411 --LKMHVKPNAPILGALLGACKIYKNTEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQ 470

Query: 304 EMAAKEVRKFFAEARMKKTPGCSVLEVDGAVQEFLAG-DLSHPQVQDI 351
               + +R    E  +KK PG S++E+DG + +F  G D  HP++  I
Sbjct: 471 WDKIESLRDMMKEKLVKKPPGWSLIEIDGKINKFTMGDDQKHPEMGKI 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJZ32.3e-7640.06Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
Q9C8663.3e-7540.91Pentatricopeptide repeat-containing protein At1g31430 OS=Arabidopsis thaliana OX... [more]
Q9FJY78.1e-7440.57Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
Q9FI801.4e-7337.06Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX... [more]
Q9FG161.9e-7038.79Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_022154784.13.54e-255100.00pentatricopeptide repeat-containing protein At5g66520-like [Momordica charantia][more]
XP_038875707.15.01e-22787.82pentatricopeptide repeat-containing protein At5g66520-like [Benincasa hispida] >... [more]
XP_022922110.14.10e-22687.82pentatricopeptide repeat-containing protein At5g66520-like [Cucurbita moschata] ... [more]
KAG6579406.15.82e-22687.82Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_023549650.18.25e-22688.10pentatricopeptide repeat-containing protein At5g06540-like [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1DN731.71e-255100.00pentatricopeptide repeat-containing protein At5g66520-like OS=Momordica charanti... [more]
A0A6J1E2B11.98e-22687.82pentatricopeptide repeat-containing protein At5g66520-like OS=Cucurbita moschata... [more]
A0A6J1HYB27.64e-22487.25pentatricopeptide repeat-containing protein At5g48910-like OS=Cucurbita maxima O... [more]
A0A5A7T9N45.26e-21483.57Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3ATN35.26e-21483.57pentatricopeptide repeat-containing protein At5g66520-like isoform X1 OS=Cucumis... [more]
Match NameE-valueIdentityDescription
AT2G22410.11.6e-7740.06SLOW GROWTH 1 [more]
AT1G31430.12.3e-7640.91Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT5G66520.15.8e-7540.57Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G48910.19.8e-7537.06Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G06540.11.3e-7138.79Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 48..77
e-value: 4.2E-5
score: 23.5
coord: 17..47
e-value: 3.3E-8
score: 33.2
coord: 121..141
e-value: 0.084
score: 13.2
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 147..193
e-value: 3.7E-8
score: 33.4
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 48..82
e-value: 2.4E-8
score: 31.6
coord: 151..178
e-value: 1.3E-5
score: 23.1
coord: 17..47
e-value: 2.4E-7
score: 28.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 15..49
score: 11.761533
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 147..181
score: 10.533867
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 119..330
e-value: 1.7E-33
score: 118.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..105
e-value: 3.4E-26
score: 93.7
NoneNo IPR availablePANTHERPTHR47926:SF162SUBFAMILY NOT NAMEDcoord: 16..339
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 16..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1297.1MC06g1297.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding