MC06g1265 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1265
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationMC06: 18904185 .. 18909761 (-)
RNA-Seq ExpressionMC06g1265
SyntenyMC06g1265
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTATGTGTTTTTGGTTTTCCTATATGGTGTGAAGATCAGCATTACTCTTTGTATCTGTAATCAGGTTATATAACCTATCTTTCTTTCTTTGATACTTCCAAGAGTGGGTTTTCATTTCAGTTATTTTTGTCAACTGCTATATCTTTTTTATTTTTTCTTTTTTAGTTCGGGTTCATTTCAATTTTAAATGCTTGAGGTGTTACTTGTGGCATAGTTATTAGGTAAGATTCAAACTGAGGCATGGGCACCAAATTTTTTGCTTGAAAGTTTTATGTATCTTGTGGATTTATGGAATTTTTGAGTGAGTTTTGTCTAACATATCACCATTTTGTTCATGGGATTCTTTCCTACCCTGTGTATATGGCAGTCTTGTTGTTTTGCTTATTTTTTTAATTCAAATTTAAGACTGAAATGTCAATTTATCCCTCAGGTTGATGTCTAACCCATGGATACAATAAAATGTCGACATGTCGATGGATATTTAATACCCTGGTTATTCATATGATTGTCACATTAATGATATTTTGTTCATATTTTTTTATACTTCTTTGATTTTAACAATATGGGATTCATATTACAGGGTAATTAATTTCTTGTGCAAATTTCAGATGCTGAGATTAAATGAATTATAGAATATGGGATTCATATTAAAGACTATGTTGCATATCTTCCTTGGTTTTGTTATAGATTAATAACTTGAAATTTATTCCAGGAACTTCCGTTTTGCTATTCAACAGTTCCTGCTGCTGCCTACTATAATTATGGAGATAAAGACAGATAGTAATGATGGCTCACTGATTTTAATCAAGCAAGGAGCTGAAGCTGTGAGTCGAAACTTCCAGTTGTCCATTAATCTGGGTTGTTCTTTGGAAAATGGAGAACTATGGAAAATGGAATATATGGTTTAAGTTCTTCAAGAGTGAGTTTGGGATGATTTTTGAGAGATTAGTGCTTATTATATAAACAATCTTGAAAGAAACACTAATTAAGATTATTATAGTTTTCAAAACTACTTTTTAACTTTATAACCAAACGCCGTGAAGAATTTGAAAATATTTTCCATCATTCCGAAAGTCATGTCAAACTAATTCTAAGAATTTTAGTGAGATATTGTGATGGTATCTTAGTGGTCTTAAGATATTCTTTTTCATCGTTTGTTCTTATCTTGCAGAGAGTGTTTGAGTCTACTTTTGTTGGAAGGAGATCGATCATTAAGGAACGCTTCTCAAAGAAATATAGACATCCATCACTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGTTTGTTTTCCTTCTTAAGAGACACGTTTTATAGTTAAAGAAGTAACTAGCTAAAAGATTTTCTTTCATATGTTTGACTGAAACTTCATGTTAGGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTTTCCACTCCAGTGCTGTATGCTGTTGACCCGACGCTACATACCCTGACATTTGAGTATGTGGAAGGCCGTGCTGTGAAAGATATATTGCTTGAAATCGGATCAAGTGGTGATGGTGCAAAACAGTTGAATGACATTGCAATGCAGATTGGTGTTGCAATTGGAAAGTTGCATGATGGTGGTTTAGCTCATGGTGACTTGACCACATCTAACATGTTGATCAGGAGTGGTACCAATGAACTCGTGAGTGGCATCTTTATTATAGTTTACAAAGAGAGACATTTTAATCTATCTTGCTAGATTTTTAATAATATGATCTCTGTCATAAGGGCAATTGTCTTTTCTCAGGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACGATTCCAGAAGATAAAGCAGTTGATCTATATGTCTTGGAACGAGCGCTGCTTTCAATGCATTCTTCATGTGGGAATTTGGTAAGTTGTTCCCCTTGCATTTGCTTTGCTATAAAAAATTCTTTCTTATGTTCTTCAATAAGACATTATTTTTCTATAAACAAAAATATTGTGTCTGGCATTGTAGTTTGCTATGCACCTGTTTGTATTGATAGAATTAGTGATTGTAGGAAGAAATGAGAGTTCTTCATCACACTCCGTTTAGGGATTGGGGCCAATTCTTATGGCAGGCAGATGGGTATCTTTAGCTGTTCTATGGGATCTGTGGATCGAGAGAAATAACATGATTTTTAGAGGGGTAGAGAGATCTTGGGAGGATGTTTATGATCGTGTCAGTGTTTTCGAGGCGTAAGGCGCACCGCAAGGCGACATGCCCTTTGATCGCCTTGAGGCGAGAGGCGATACAAAGGCGACACCCAATTGAAGCGAGGCGCACCTTAATAAATAAGTAAAAATTATATATATATTGCATAAATTACCTTAAAAAAATTAATATCAAAATAGTAAATGTCAACAAGATACATAAAACAACCAAATTCTATAAGTTTAGTTCCAAATAAAGACAAGCAACAGCGGAGATTGATGGTGGTGAAGGCTTGAAGCCCGTGAAGGTGAATGTCGAGAGGGAGGGGAGGCAGTGATAGGGTTTGGGTCGCGAAGGGGAGGGTGTTTTACAAAAACAACACATAAAAAATTAAAAAAAAATTTAAAAATAAATAAGGCAGATCGCCCCCTCGAAGGAGGCGATCGCCTTGCCTCAAGCGACAGGCGCACCAAGGCGCCCAAAGGCGAGCGCCCGATTGAAGGCGCTCGCCTGGGCACCTGGGCGAAAACGCCTCGCCTCTCGCCTGAGGCCAGCGCTCGGGTGCGCCTCGAAAACACTAGATCTTGTTCGGTTTCAGGTTTCCCTTTGTGCCTCGGTATCCAAAGCCTTTTGTAATTATTCATTATATTTAATTTTGTTTGATTGAAGCTTGTTGTTTTATTTATTTATTTATTCTTTAAGTAAGAAACTTGTATTTCATTGATGATATGAAATATACAAAAGGCCATAAAGGTAAGTTACAAAAACGCCTTCCAATTTCCTATAAGGCCATTTAAGTTGTAACTAATGAAAGGGGAGTAGAAATATTTACACCATTGTAGGGCGAGTACAATAACTTTATCAAAAAAGATTTCAAAAGGTAGAGAGGAGTTGTTAAAAATCCTATTGTTCCTTTCCATCCACAACCTCCATAGGTAAGCTCTAATGATTTGGGCCCAAAGGATTGCGCTCACTTTTTTGAAAGAGTGACCAGTCATGAGAAAGTTTAGGAAGATCTTTGGGTCATTGTGTAATGCAGTTTGCCACCCAAAAGCTGTTAGAATCTTCTCCCAAAATCTGGATGCGTAGGCACACGTGATAAAGAGGTGACCTTGAGTTTCAGATGCCATATAGCAAAGATGGCAGCAGTTTGGGGAGAGGGCCATGTGTGGTAGCCGTCTTTGGACCCTGTCATGTGTGTTGATCGTGCAATGACTTAGCTCCCAGCAGAAAATCTCGATTTTCTTAGGATATTTCCCATGCCAGATGCTCTGATAGAGGCGAGGAGGCTGGCTATTCTTAGTGGAGGTCTACAGAGAGAAGACAGACTTTGTTCTGTATAGTTTTGTATGATCCTGTCTCCATATCCATTCATCGTCGATTGGGGAGGGACAAAGCTGTTTAGAACTTGTGTAAGTTGCAGCCAATCCTCTAACTCTTCATCTCTATGGTCGGCGAAAGTTGTAATTCCAAGATGCTGTGGTCGTGGACCAAAGATCCATGATTTTGGCGTCTTTGTTCATGGTGATAGCGTAGAGTCTGCAGAAGCGGGCGGTCAAAATTTCTGAATTTAACCAATTATCATTCCAGAAGCTTAGTTGGCTGTTTTCTCCAATATGGCAGTAGGCATTTGCCTTAATAATGTGTGTTTGCTGAAGGATAGCACACCAAGGGCCTTTGTATAAGCTGAATCGGTTTGGGGGCCAGATTGCTGAGGGGTCCCACCATACTTTGCCAAAATGTATTTCCTCCAAAGCTGATTTGCCTCTGTAATAAAGCGCCAAATGCACTTGGACAGCAGTGCGATGTTTCTTTTCTTAATTTGCCTCTCGTTGTTTTAGTTTGGCTCTTTTTTATGCTCTTTTGCTTTGTTTCATTCTACTTGATTAAAGTTCGGTTTTTTCTCCCAAAAATAAAATGAAAAAATAAGAGTTATATTCTACTGAATTGAAGGAAAGTACCCGAATAGATGTACGTGTAGCCGAAACTTGAGACCCCTTAGCTTCTCTCCTGCAAGCTAAGATCAGCTGAATAACTGAAGAAGTTAATAAACTTCCCAGTTTCTGTTAAATGTATTTTGGCCCATCTTGCATGCAACTTTATCATTATCTCATGCCCCCTTGCCCATTCCTCCAACTATTCTCTCTATATATTGCCAATCCTAGAAACTACTCGAAAAGATTTAACTAGAAATTGAGATTCGTCCTTGGATTTTTAGATGAGTTAGAGAGATCTTGAAGAGGATGTTTGAGCTCTGGTTCTTTTTCATGTCTCCCTTTTGGGCTTGGTGTCCAAAGCCTTTTGTTTTTCTTTGTTAGGTTTGATCGATTGGAGCCCCCTTTTAGTTTGCCTCCCTTTTGTTGGGCGGTTTATTTATACGCCTTTTTGTTTCATTTCCTTGTCATCAATGACAGTTCAGTCTTTTCTTAAAAAAGAAATTGAGATTTGCAACTTTACTGCTATCTCATGCCCCTTGCCCACATATTCTAACTATTCTCTATATAATTCTTCATTTTCTCTTATTCATAACTTTCCTGAATTACCCTTATATCAGTTGAATGTTTGCTAATCTCGGTCTAATTATTAAAATATACAAGTTAGGGTAGGAGTCCTATCATAATTCTAGAATGGGGATTCCCTCGCTAAGAAGTTTAGATACTGACACGAGACACAGATATGACACGAAACAGGCACAATAATATGTAATTTTCTAAAGATCTAGGACACAAACACGTTTCTTAAAATATATTTCATTTTTATACCATAAGGAAATTCAAAGTCCATAAGTTTTATGCATTTTTATACTTAAAAAAATAAGTTTGTTATATTTTTTTCTCGAAATTTTGTTATTATGTCCCATATCACTATGTCTATTTTTTATTCTTAACAAGTGTTCAATACATGCCTATAACAAGTGTCAAAGTAGAGTAATTTTGGCATTGTAATCTATAACTACTTGTTATTGACAAATTGGGTTGAAATGAAATTCAAATCAATGTACTTTGATGGTTGTACATATTGTATAGATGGAGCTGATTCTTGCTTCATACCGAAAGACGTCGAAACAATGGTCATCGACCTCAAACAAATTAGCTCAAGGTAGCAAACCCCGACTTTTATCATTCACTTTTTTACTTTCATATCAATTTCATTGCTATGATTCATTAGTTTACTTTTCTTGTTTTCACATTGATGCACATACAGTTCGTCAGAGAGGCCGAAAACGTACGATGGTTGGTTAAGATCTTTCTTTTTGGTCTGTTTGTTGAATTATTTGTAGACTCATTGAACTTTAACAGTGTATTATTATTAACCTTACAATAACTATTTTTTTCGTAATTGTTAGTTTGTGGAAAAAACTTGTATTTACTATTTGGTTTTAGTAAACAGTAATAGTGATTTAGTATTCTTCAACA

mRNA sequence

GCTATGTGTTTTTGGTTTTCCTATATGGTGTGAAGATCAGCATTACTCTTTGTATCTGTAATCAGGAACTTCCGTTTTGCTATTCAACAGTTCCTGCTGCTGCCTACTATAATTATGGAGATAAAGACAGATAGTAATGATGGCTCACTGATTTTAATCAAGCAAGGAGCTGAAGCTAGAGTGTTTGAGTCTACTTTTGTTGGAAGGAGATCGATCATTAAGGAACGCTTCTCAAAGAAATATAGACATCCATCACTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTTTCCACTCCAGTGCTGTATGCTGTTGACCCGACGCTACATACCCTGACATTTGAGTATGTGGAAGGCCGTGCTGTGAAAGATATATTGCTTGAAATCGGATCAAGTGGTGATGGTGCAAAACAGTTGAATGACATTGCAATGCAGATTGGTGTTGCAATTGGAAAGTTGCATGATGGTGGTTTAGCTCATGGTGACTTGACCACATCTAACATGTTGATCAGGAGTGGTACCAATGAACTCGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACGATTCCAGAAGATAAAGCAGTTGATCTATATGTCTTGGAACGAGCGCTGCTTTCAATGCATTCTTCATGTGGGAATTTGATGGAGCTGATTCTTGCTTCATACCGAAAGACGTCGAAACAATGGTCATCGACCTCAAACAAATTAGCTCAAGTTCGTCAGAGAGGCCGAAAACGTACGATGGTTGGTTAAGATCTTTCTTTTTGGTCTGTTTGTTGAATTATTTGTAGACTCATTGAACTTTAACAGTGTATTATTATTAACCTTACAATAACTATTTTTTTCGTAATTGTTAGTTTGTGGAAAAAACTTGTATTTACTATTTGGTTTTAGTAAACAGTAATAGTGATTTAGTATTCTTCAACA

Coding sequence (CDS)

ATGGAGATAAAGACAGATAGTAATGATGGCTCACTGATTTTAATCAAGCAAGGAGCTGAAGCTAGAGTGTTTGAGTCTACTTTTGTTGGAAGGAGATCGATCATTAAGGAACGCTTCTCAAAGAAATATAGACATCCATCACTGGATTCCAAACTCACACTCAAGCGATTGAATGCAGAAGCAAGGTGCATGACAAAAGCTAGGCGACTTGGAGTTTCCACTCCAGTGCTGTATGCTGTTGACCCGACGCTACATACCCTGACATTTGAGTATGTGGAAGGCCGTGCTGTGAAAGATATATTGCTTGAAATCGGATCAAGTGGTGATGGTGCAAAACAGTTGAATGACATTGCAATGCAGATTGGTGTTGCAATTGGAAAGTTGCATGATGGTGGTTTAGCTCATGGTGACTTGACCACATCTAACATGTTGATCAGGAGTGGTACCAATGAACTCGTCCTTATTGATTTTGGTCTGAGCTTTACTTCAACGATTCCAGAAGATAAAGCAGTTGATCTATATGTCTTGGAACGAGCGCTGCTTTCAATGCATTCTTCATGTGGGAATTTGATGGAGCTGATTCTTGCTTCATACCGAAAGACGTCGAAACAATGGTCATCGACCTCAAACAAATTAGCTCAAGTTCGTCAGAGAGGCCGAAAACGTACGATGGTTGGTTAA

Protein sequence

MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQIGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Homology
[Close]
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match: Q54W07 (EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.9e-48
Identity = 103/214 (48.13%), Postives = 135/214 (63.08%), Query Frame = 0 

Query: 13  ILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRLGV 72
           ILI QGAEA+ +E+   G + I+KERFSK YRHP LD K++ KR+  E R + K ++ G+
Sbjct: 39  ILISQGAEAKTYETDLYGLKCIVKERFSKAYRHPILDQKISSKRILQEVRSLNKCKKKGI 98

Query: 73  STPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQIGVAIGKLHDGG 132
             P LY VD   + +  E+++G  VK  L +   S     Q+  I  ++G  IG +H+  
Sbjct: 99  QVPSLYLVDIGNNRIYMEFIKGETVKHYLYKNQESTQHQNQIESIMKELGNQIGIIHEMN 158

Query: 133 LAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCGNLME 192
           + HGDLTTSNML+R  TNELV IDFGLS+TS   EDKAVDLYVLERA +S H +   L +
Sbjct: 159 VIHGDLTTSNMLLRESTNELVFIDFGLSYTSNSVEDKAVDLYVLERAFISTHPNSEQLFQ 218

Query: 193 LILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
            IL++Y  TS        KL QVR RGRK+T  G
Sbjct: 219 TILSNYELTSSNSKIVIQKLNQVRLRGRKKTCFG 252

[Close]
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match: Q96S44 (EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 6.0e-46
Identity = 104/218 (47.71%), Postives = 140/218 (64.22%), Query Frame = 0 

Query: 12  LILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRLG 71
           L L+KQGAEARVF   F GR ++IK RF K YRHP+L+++L  +R   EAR + + RR G
Sbjct: 36  LELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAG 95

Query: 72  VSTPVLYAVDPTLHTLTFEYVEGR-AVKDILLEIGSSGDGAKQLNDIAMQIGVAIGKLHD 131
           +S PV++ VD   + L  E +EG   V+D +     +    + L+++A  IG  + ++HD
Sbjct: 96  ISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQVLARMHD 155

Query: 132 GGLAHGDLTTSNMLIRSGTNEL--VLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCG 191
             L HGDLTTSNML++    +L  VLIDFGLSF S +PEDK VDLYVLE+A LS H +  
Sbjct: 156 EDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTE 215

Query: 192 NLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
            + E  L SY  +SK+      KL +VR RGRKR+MVG
Sbjct: 216 TVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG 253

[Close]
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match: Q99PW4 (EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 7.3e-44
Identity = 102/218 (46.79%), Postives = 136/218 (62.39%), Query Frame = 0 

Query: 12  LILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRLG 71
           L L++QGAEARVF   F GR +++K RF K YRHP L+++L  +R   EAR + + RR G
Sbjct: 27  LELVQQGAEARVFRGRFQGRAAVVKHRFPKSYRHPELEARLGRRRTVQEARALLRCRRAG 86

Query: 72  VSTPVLYAVDPTLHTLTFEYVEGR-AVKDILLEIGSSGDGAKQLNDIAMQIGVAIGKLHD 131
           ++ PV++ VD   + L  E +E    V+D +     +    + L D+A ++G  +  +HD
Sbjct: 87  IAAPVVFFVDYASNCLYMEEIEDSVTVRDYIQSTMETEKDPQCLLDLARRMGQVLAGMHD 146

Query: 132 GGLAHGDLTTSNMLIRSGTNEL--VLIDFGLSFTSTIPEDKAVDLYVLERALLSMHSSCG 191
             L HGDLTTSNML+R    +L  VLIDFGLSF S +PEDK VDLYVLE+A LS H    
Sbjct: 147 QDLIHGDLTTSNMLLRRPLAQLHIVLIDFGLSFVSGLPEDKGVDLYVLEKAFLSTHPHTE 206

Query: 192 NLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
              E  L SY  +SK+ S    KL +VR RGRKR+MVG
Sbjct: 207 TAFEAFLKSYGASSKKSSPVLKKLDEVRLRGRKRSMVG 244

[Close]
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match: Q4HYC1 (EKC/KEOPS complex subunit BUD32 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=BUD32 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.9e-37
Identity = 100/229 (43.67%), Postives = 134/229 (58.52%), Query Frame = 0 

Query: 13  ILIKQGAEARVFESTFV--GRRSIIKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRL 72
           ILI QGAE R++++T++       +K R  K +RHP LD +LT  R+ +EAR + K RR 
Sbjct: 34  ILITQGAEGRLYKTTYLLPDIPCALKYRPPKPWRHPILDQRLTKHRILSEARILAKCRRD 93

Query: 73  GVSTPVLYAVDPTLHTLTFEYVEGRAV-KDILLEIGSSGDGAK---QLNDIAMQIGVAIG 132
           GV  P +YAVD +   L  E+V G  V K I   +G+  +G +   +L D+  +IG AIG
Sbjct: 94  GVRVPAVYAVDESAGWLMLEWVSGGPVRKSINERLGNRTEGIENDAELKDLMRRIGTAIG 153

Query: 133 KLHDGGLAHGDLTTSNMLIRSGTN---------ELVLIDFGLSFTSTIPEDKAVDLYVLE 192
            +H  G+ HGDLTTSNM++    N         ELV+ID GLS  S   ED+AVDLYVLE
Sbjct: 154 NMHKVGIVHGDLTTSNMMLEPLANPQDDNPLHGELVIIDLGLSSGSISDEDRAVDLYVLE 213

Query: 193 RALLSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           RA  S H     +   +L +Y +T KQ      KL  VR RGRKR+M+G
Sbjct: 214 RAFGSTHPRAECVFSEVLDAYGQTFKQAKVVLKKLEDVRMRGRKRSMLG 262

[Close]
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match: Q9P7N1 (EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP27G11.07c PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.9e-37
Identity = 91/227 (40.09%), Postives = 135/227 (59.47%), Query Frame = 0 

Query: 2   EIKTDSNDGSLILIKQGAEARVFESTFV-GRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 61
           +I  +  +  L ++KQGAEA   ++ F  G   ++K R +K++RHP LD KL+ KR   E
Sbjct: 13  DIYREIKEKKLTVVKQGAEAITIKTEFYPGEVCLLKCRPAKRWRHPILDQKLSRKRCLVE 72

Query: 62  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 121
           AR + K   +G+  P+LY +D     +  E+++G  V+D + EI    +  K+L  +  +
Sbjct: 73  ARLLAKCHYVGIKCPMLYFIDANRGQIYMEWIDGPCVRDYIREI-CECEIEKKLIPLMKR 132

Query: 122 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNEL-VLIDFGLSFTSTIPEDKAVDLYVLERA 181
           IG  + K+H   + HGDLTTSNM++ S  N + + IDFGL   S   EDKAVD+YVLERA
Sbjct: 133 IGSEVAKMHKNDIVHGDLTTSNMMLESHNNPVPIFIDFGLGSVSESEEDKAVDIYVLERA 192

Query: 182 LLSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           L S      +L   +L SY ++ KQ  +T  +  +VR RGRKRTM+G
Sbjct: 193 LSSTLPESESLFHHVLDSYAQSWKQSKATLRRFEEVRMRGRKRTMIG 238

[Close]
BLAST of MC06g1265 vs. NCBI nr
Match: XP_022154600.1 (EKC/KEOPS complex subunit bud32 [Momordica charantia] >XP_022154601.1 EKC/KEOPS complex subunit bud32 [Momordica charantia])

HSP 1 Score: 433 bits (1114), Expect = 7.32e-153
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ
Sbjct: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. NCBI nr
Match: XP_022971395.1 (EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima] >XP_022971403.1 EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima])

HSP 1 Score: 415 bits (1067), Expect = 1.07e-145
Identity = 214/226 (94.69%), Postives = 220/226 (97.35%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP LHTLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. NCBI nr
Match: XP_023529406.1 (EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 413 bits (1061), Expect = 8.79e-145
Identity = 213/226 (94.25%), Postives = 220/226 (97.35%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP L+TLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. NCBI nr
Match: KAG6588447.1 (EKC/KEOPS complex subunit bud32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 411 bits (1056), Expect = 1.34e-143
Identity = 212/226 (93.81%), Postives = 219/226 (96.90%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           ME KTDSNDGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 28  METKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 87

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP L+TLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 88  ARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 147

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 148 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 207

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 208 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 253

[Close]
BLAST of MC06g1265 vs. NCBI nr
Match: XP_022924768.1 (EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924777.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924786.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924793.1 EKC/KEOPS complex subunit bud32 [Cucurbita moschata])

HSP 1 Score: 408 bits (1048), Expect = 8.42e-143
Identity = 211/226 (93.36%), Postives = 218/226 (96.46%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           ME KTDS DGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   METKTDSYDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP L+TLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match: A0A6J1DK25 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111021824 PE=3 SV=1)

HSP 1 Score: 433 bits (1114), Expect = 3.54e-153
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ
Sbjct: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match: A0A6J1I5M0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111470128 PE=3 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 5.18e-146
Identity = 214/226 (94.69%), Postives = 220/226 (97.35%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP LHTLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILHTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match: A0A6J1EDH5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111432168 PE=3 SV=1)

HSP 1 Score: 408 bits (1048), Expect = 4.08e-143
Identity = 211/226 (93.36%), Postives = 218/226 (96.46%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           ME KTDS DGSL+LIKQGAEARVFESTFVGRRSI+KERFSKKYRHPSLDSKLTLKRLNAE
Sbjct: 1   METKTDSYDGSLVLIKQGAEARVFESTFVGRRSIVKERFSKKYRHPSLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRL VSTPVLYAVDP L+TLTFEYVEGR+VKDILLEIGSSGDG+KQL DIAMQ
Sbjct: 61  ARCMTKARRLAVSTPVLYAVDPILYTLTFEYVEGRSVKDILLEIGSSGDGSKQLKDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNML+RS TNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLVRSSTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LSMHSSCGN+ME ILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSMHSSCGNVMEQILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match: A0A1S3BPU1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103492219 PE=3 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 3.35e-142
Identity = 209/226 (92.48%), Postives = 220/226 (97.35%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDG+LIL+KQGAEARVFESTFVGRRSI+KERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRLGV+TPVLYAVDP L+TLTFEYVEG +VKDILLEIGS+G  +K+L+DIAMQ
Sbjct: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LS+HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match: A0A5D3CF78 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003400 PE=3 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 3.35e-142
Identity = 209/226 (92.48%), Postives = 220/226 (97.35%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           MEIKTDSNDG+LIL+KQGAEARVFESTFVGRRSI+KERFSKKYRHP LDSKLTLKRLNAE
Sbjct: 1   MEIKTDSNDGTLILVKQGAEARVFESTFVGRRSIVKERFSKKYRHPLLDSKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKARRLGV+TPVLYAVDP L+TLTFEYVEG +VKDILLEIGS+G  +K+L+DIAMQ
Sbjct: 61  ARCMTKARRLGVATPVLYAVDPILYTLTFEYVEGSSVKDILLEIGSNGGDSKRLSDIAMQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IGVAIGKLHDGGL HGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL
Sbjct: 121 IGVAIGKLHDGGLIHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226
           LS+HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG
Sbjct: 181 LSLHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 226

[Close]
BLAST of MC06g1265 vs. TAIR 10
Match: AT5G26110.1 (Protein kinase superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 3.4e-97
Identity = 177/226 (78.32%), Postives = 202/226 (89.38%), Query Frame = 0 

Query: 1   MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
           M+ + +  D SL+LIKQGAEARVFESTF GRRSI+KERFSKKYRHP LD+KLTLKRLNAE
Sbjct: 1   MDCEENVRDESLVLIKQGAEARVFESTFAGRRSIVKERFSKKYRHPILDAKLTLKRLNAE 60

Query: 61  ARCMTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQ 120
           ARCMTKAR+LGV TPVLYAVD  LH+LT EY+EG +VKDI LE G++G   ++L+D+A Q
Sbjct: 61  ARCMTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDIFLEFGTNGVVEERLDDVAAQ 120

Query: 121 IGVAIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERAL 180
           IG AI KLHDGGLAHGDLTTSNML+RSGTN+LVLIDFGLS TST+PEDKAVDLYVLERAL
Sbjct: 121 IGAAIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLSVTSTLPEDKAVDLYVLERAL 180

Query: 181 LSMHSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           LSMHSSCGN+M+ IL +YRK+SKQWS+T NKLAQVRQRGRKRTM+G
Sbjct: 181 LSMHSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGRKRTMIG 226

[Close]
BLAST of MC06g1265 vs. TAIR 10
Match: AT5G26110.2 (Protein kinase superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 4.4e-68
Identity = 127/163 (77.91%), Postives = 146/163 (89.57%), Query Frame = 0 

Query: 64  MTKARRLGVSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQIGV 123
           MTKAR+LGV TPVLYAVD  LH+LT EY+EG +VKDI LE G++G   ++L+D+A QIG 
Sbjct: 1   MTKARKLGVCTPVLYAVDTLLHSLTLEYIEGVSVKDIFLEFGTNGVVEERLDDVAAQIGA 60

Query: 124 AIGKLHDGGLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPEDKAVDLYVLERALLSM 183
           AI KLHDGGLAHGDLTTSNML+RSGTN+LVLIDFGLS TST+PEDKAVDLYVLERALLSM
Sbjct: 61  AIAKLHDGGLAHGDLTTSNMLVRSGTNQLVLIDFGLSVTSTLPEDKAVDLYVLERALLSM 120

Query: 184 HSSCGNLMELILASYRKTSKQWSSTSNKLAQVRQRGRKRTMVG 227
           HSSCGN+M+ IL +YRK+SKQWS+T NKLAQVRQRGRKRTM+G
Sbjct: 121 HSSCGNVMDRILTAYRKSSKQWSATFNKLAQVRQRGRKRTMIG 163

[Close]
BLAST of MC06g1265 vs. TAIR 10
Match: AT1G08120.1 (BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (TAIR:AT5G26110.1); Has 235 Blast hits to 235 proteins in 96 species: Archae - 21; Bacteria - 0; Metazoa - 117; Fungi - 8; Plants - 47; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink). )

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-16
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 0 

Query: 1  MEIKTDSNDGSLILIKQGAEARVFESTFVGRRSIIKERFSKKYRHPSLDSKLTLKRLNAE 60
          M+ + +  + SL+LIKQGAEARV ESTF GRRSI+KERFSKKYRHP LD+KLTLKRL  +
Sbjct: 5  MDCEENVGNESLVLIKQGAEARVLESTFSGRRSIVKERFSKKYRHPILDAKLTLKRLYDK 64

Query: 61 ARCMTKARRL 71
           +   KAR L
Sbjct: 65 GK---KARGL 71

[Close]
BLAST of MC06g1265 vs. TAIR 10
Match: AT5G01850.1 (Protein kinase superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 3.4e-04
Identity = 39/156 (25.00%), Postives = 69/156 (44.23%), Query Frame = 0 

Query: 15  IKQGAEARVFESTFVGRRSI---IKERFSKKYRHPSLDSKLTLKRLNAEARCMTKARRLG 74
           I +GA  +V++  + GR+ +   +  R SK  +  SL+S     R   E   M++ +   
Sbjct: 24  IGEGAHGKVYQGRY-GRQIVAIKVVNRGSKPDQQSSLES-----RFVREVNMMSRVQHHN 83

Query: 75  VSTPVLYAVDPTLHTLTFEYVEGRAVKDILLEIGSSGDGAKQLNDIAMQIGVAIGKLHDG 134
           +   +    DP +  +T E + G +++  L  I             A+ I  A+  LH  
Sbjct: 84  LVKFIGACKDPLMVIVT-ELLPGMSLRKYLTSIRPQLLHLPLALSFALDIARALHCLHAN 143

Query: 135 GLAHGDLTTSNMLIRSGTNELVLIDFGLSFTSTIPE 168
           G+ H DL   N+L+      + L DFGL+   ++ E
Sbjct: 144 GIIHRDLKPDNLLLTENHKSVKLADFGLAREESVTE 172

The following BLAST results are available for this feature:
BLAST of MC06g1265 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q54W072.9e-4848.13EKC/KEOPS complex subunit bud32 OS=Dictyostelium discoideum OX=44689 GN=bud32 PE... [more]
Q96S446.0e-4647.71EKC/KEOPS complex subunit TP53RK OS=Homo sapiens OX=9606 GN=TP53RK PE=1 SV=2[more]
Q99PW47.3e-4446.79EKC/KEOPS complex subunit Tp53rk OS=Mus musculus OX=10090 GN=Tp53rk PE=2 SV=1[more]
Q4HYC13.9e-3743.67EKC/KEOPS complex subunit BUD32 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 1... [more]
Q9P7N13.9e-3740.09EKC/KEOPS complex subunit SPAP27G11.07c OS=Schizosaccharomyces pombe (strain 972... [more]
BLAST of MC06g1265 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022154600.17.32e-153100.00EKC/KEOPS complex subunit bud32 [Momordica charantia] >XP_022154601.1 EKC/KEOPS ... [more]
XP_022971395.11.07e-14594.69EKC/KEOPS complex subunit bud32 isoform X1 [Cucurbita maxima] >XP_022971403.1 EK... [more]
XP_023529406.18.79e-14594.25EKC/KEOPS complex subunit bud32 [Cucurbita pepo subsp. pepo] >XP_023529407.1 EKC... [more]
KAG6588447.11.34e-14393.81EKC/KEOPS complex subunit bud32, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022924768.18.42e-14393.36EKC/KEOPS complex subunit bud32 [Cucurbita moschata] >XP_022924777.1 EKC/KEOPS c... [more]
BLAST of MC06g1265 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DK253.54e-153100.00Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1I5M05.18e-14694.69Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EDH54.08e-14393.36Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3BPU13.35e-14292.48Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5D3CF783.35e-14292.48Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
BLAST of MC06g1265 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G26110.13.4e-9778.32Protein kinase superfamily protein [more]
AT5G26110.24.4e-6877.91Protein kinase superfamily protein [more]
AT1G08120.11.7e-1665.71BEST Arabidopsis thaliana protein match is: Protein kinase superfamily protein (... [more]
AT5G01850.13.4e-0425.00Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022495Serine/threonine-protein kinase Bud32TIGRFAMTIGR03724TIGR03724coord: 14..220
e-value: 5.4E-60
score: 200.2
IPR022495Serine/threonine-protein kinase Bud32PANTHERPTHR12209O-SIALOGLYCOPROTEIN ENDOPEPTIDASEcoord: 1..226
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 20..92
e-value: 1.5E-26
score: 93.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 108..223
e-value: 6.8E-34
score: 118.5
NoneNo IPR availablePANTHERPTHR12209:SF1EKC/KEOPS COMPLEX SUBUNIT BUD32-LIKE ISOFORM X1coord: 1..226
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 39..161
e-value: 5.5E-10
score: 39.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..226
score: 12.117799
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 14..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1265.1MC06g1265.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0008033 tRNA processing
biological_process GO:0070525 tRNA threonylcarbamoyladenosine metabolic process
cellular_component GO:0000408 EKC/KEOPS complex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity