MC06g1262 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1262
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionvesicle-associated protein 1-2-like isoform X2
LocationMC06: 18854020 .. 18860143 (+)
RNA-Seq ExpressionMC06g1262
SyntenyMC06g1262
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGCATTCAAATTTTAGTAAATAAACACTAAAATGGCCACTTTTTGAACAAACAATTTTTTAAAATACAAAGACTCTCTCTCTCTCCATCTTTCATTTCCTCGGTCTTGAATTATATAGAGAGTTAATTCCACGAAGAACTTTGGCGCTATTTAGTATGTCCATTCCCTCACCGAGCTTTAATTTGTTCGTCCACAACAGATTCTCTTTCGCGAATCAGTTCTATTCGCTTCTCCACTTCTTTTTTCCTTTTTTTTTTCTGTTCGTTTCTGATTCCCGGTTCTAATCTTTTTCGTGCTGGATTTATGTGAAGAAACTCTGTTGGTTGAGAACGAGTGATCCAGTTCACTCACTCACTCCGTTTGCTTCTTCTTCTTCTTTGTTTCACTTTTTGAGTTGAGAATGAGTTCGTATAATTTTGAATTCTGGTGACTGGACGAACTCATTGTTCGATCGCTTTCGTGGTGAAACATTGAAGCAAATTTCTCGTCGATTGTGAGTTTTTTTACATATCGAGGGAAGATTAAATTGAAATTTTCACCTGATCTTACTAAATTTTCTTTCTAGTTAATCGGTATAGAGCTGAATTCAGAATAGTCTATTAATTTAGTCTGAAAACGATTTTAGAGTATTAGTATTTGTTTGTTGTGAAAATCTTATGGGGTCTTAAATAAATTAATTCGCTTATTTGTTCTAATATGGATGATCGTCTTCTGTTTTTTGTTTTTAGTCTGGGCTGCGATGATCATGTTCAATTGGGGTTTTTCTTTTATATATCAATGCCCCCAAATTCTCTGTTTTAGTCATTCTGGTTTTTGTAATTTGATTTTGCAGGACAATTGTTTGATGCAATAGCCGAGTGAATTGTTTCTCAACAGAGTTGAATATGGGTATGAGACTTCTGGAAATTCATCCCCAGGAATTGAAGTTCGTCTGTAAGTCTACAGAATCAATCAATTGTTATTTATTTTCTTGTTTCCAATATGCTGGTTGCCGATGATTTCTGGAAGCATTACTGGAAATAGTTGCTGCCCTCTTTGCTCCCTGTCAATTTGATCTTGTGTGGGAAAAGCAAAGTGATTTTCTTGTTTGAATGTTTTTTTAATACTTTTTAGCTTTTGTCCCAATGTATGTTTCTTACTCTTCCATTTGTACTTTGTCTGCTTATTGATCACGGCCGAAGGGTGAGTTTTGGATTTTTAGCTGACTAGAAATATTTTTCCGAATTCTTGTTGCTGTTTGATTGCATAGGGAGGAGTGATTTTAAAAGTTATCAAAATACTTTAAAAAGTATTTCTTAGAAGAAGTATTTGAGTAAGTTTGAAATGACTTTTGGAAGAAATACTTTTAGGTGGACTTTTTTCATGAGAGAAGTAATAATAAAGTGCTTACTTTTGAAGTATTTTAAAGTATGTTAAGTATTTTTATAAAATTAAAAGCACTTTTGACTATAGTATCAATCACTATGGAAATGTTGGAAAAAAAACTGCTTTTGATTAGTTAAAATTAGTTTTCCCTCTTCTAAAAGTCATACTAAACTCATCTTTTAGTGCTTGTTATAAAAGTTTGTATTGAGAGAGTGTCTTAAAAAGTCATCCTAAGCGGAAGTTTTTATGATCAATATGAAATATGCATTTGTACATAATACTTTTTGTGATGTCCGGTATTTGATTTACTAAGCTACCTCAATTGAAGTGTCACGTCTGTTTTTCTTAATATATTTTACTGATTTACTACTAGGACCTTGTTTTCTTTTGATTTTTTATTTTGTCTGAATAAGGCACTTTATTTTTCTGTTTCACTTTATTTTCCAAATTAGTACCAAGTATTCTTCATATTTTCCTTCTATTGCCTCCAGTTGAACTAAATAAACATAGTTCGTGCTCAATTCAACTAACAAATAATACGCTTGACCATGTTGCTTTTAAGGTATGGGTTACCAATCCTCCCTCTATCGCTGTTTCTTTTCTTCTTTCTTTTTCTTCTTTTTAAAAACAAAACTTAATTAACATTATTTATGATACATTATGTAGAAATAACATGCTAGGTTGCATGCAAGATAATGGACAGAGTTTCTTTTTCAGGTTAAGACCACATCCCCTAAGAATTACTTTGTACGGCCAAAGGTCGGAATCATTGAGCCAAAATCAACCCGTGAATTCACAGGTATGTAGTTTTTGACATTTAGTTAAGTTATTTCTTTTGACTTTTGTTTCAGGAGGGGAAGGGGAGTGTATTTTATCTATAGATGTTGAAACATTGCAGCTATGAGAGTGTTCTTTCATCTTAAATAGCTTTAATGAAGCCTCAGTGAGTCTACTTTCTTCTTCTGCTTTAAACATCTCTGCCCAATTAAATTTATCATCATTTTTGGAGATGCATCTTCTTCTTATTCTTATGATTGGTTCAAGTGCTTGCACTAAATTTTTAATTGATATGGGTTTAATTTTCACATCAATGTTTTGTAATTATTGTTTTCCTGTTTGGTAACCTTCTTCTTCTTCTTATTCTTATGATCGGTTTAAGTGCTTGCATTAAACTTTTAATTGATATTGGTTTAATTTTGACATCAATGTTTTGTCATTATTGCTTTCGTGTTGGTTACCATTTTTGTTTTTTCTTAAGTTTACAAACAGTACTTTCATCTATGTGTTTTTTTTTTTGTTTTATTATATATTTTTTAAAAACATTTTTAAAATCTAAGCAAAATGTGAAAACTAAATATAGTATTTTTTTTTTTGTAACTTGTTTTTGTTTTTAAAATTTTAAAACTAAGAATTCAAGTTAAAGGTGAAAATCTCACCAAAGAAATGGCGAGAAAACTAGCATAGTTATTAAAAATGAAAAACTAAAAACAAAATTGTTATTTAAGAGGACTTCACTATCCATTTATATATCTTTTTGAGCCCCGGATTTGCCTACCTTTTTCATGCTTTCAAAATTTTATTCAATGGCTCTTAATGTAGTTAGGATGCAAGCACAAGAGATGGCTCCACCTGATATGCTATGCAAAGATAAGTTCTTAATTCAGAGCACAGTAGTTTCTGCTGATACAACTGATGAGGACATAACCTCAAGCTTGGTAAATGTTTATAGTAGTATTTACTATGTATAGTTGCCTCTGTTTTGGAGTCTCACCATTCTTTTTTTTTTTGGTGCAGTTTGCAAAAGATGTTGGCAAGTACATGGAAGAAAAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCAGCTCCCCAATTGATGAAGCATTAAAGGAGGAGTTTGATGTCGGAGTTTCAGAACCAAATAATCTATGCACAGCAGTAGAGAAGGATCTGGACCGTGAAACTTCAAACAAAAGTGATGAAATGTTCAATGTAGGTGATAGCCTGCCTCCAAAATCCATGGTTAAAATTTCTCAACTCATGTCCTTTTTACACCCTCCTCTCTCTCTCTCCCAAATTTATGCTTGATATAAAATTCGTTCACTTTCATTCCAAGTTCAGTAGGATGCTTAGGTTAGCATTCATGATACAGTGTTTTAAGAACACATTCTAACCCTGGAACATGAAAACTACTTAACACATTCTAAGTTTGGCACGTAAAAAATTGTTAAATGGTTTGCTGAATCTGTGCTTAGTTGCATATTTCATCTGTATCTAGTTTTAACCATGTGTCTTTGTTTATCAAATTATGGAATTGGAATGGCGGTTTTCATATGTATTGTACCAGGTCCCTGACAATGTTATGGAGCCGAGAGATGTTGGTAAAGGGGAGCCTGAACCGGCAAGTTTAGCTGACGACTTCATAGAAGGCGATGAGGAGGACCTGGATATGATAAAACAGGTTGAGGAGTTGAGGTTAAAGCTCACTGAACTTGAATTAAAGCTCTGTGAGGTCAGCCTTCCGCAAAATATTGCTCCCCTATTGTGAACTCTGAGCATAATGATAAAGTGGAAAAAGATGTCCAAAAGGCTTATTTTACTGTCTTAATTGAAAAGGTTCTACCAAATAGTTATTTGAGTTCTTAAACGTCTTTTATTTACAGGCTAAAGTTGCCATTTCAAAGCTGAAGGAGGAGAAGAGAATAAGCATCCAAGAAACCGAATTCTTGCAGCAGAATTTTGTAATTTCCTATACCATTATTTTCTTTTGTCTCAATCATATAATTTCCATGTTCATGTGACTCTTTTATTGTTGCCACACGGTTTATAATTTCGTAGTTGTTGGTCTGTTGAAGTCCAAATATATTTTGTTTCTGTCAACATCCTTAGATCCCTACCTGAACTTGCTCAATGTTTTCTTTGGTGGATTGAAATGAGGTTAAGTTACAAATTTAGTTCATGAATTTTTTAGTGTGATATTGATTTAGTTTCTAAATTTAAAAAGTTTTAATTATGCCTCTAAACTTTGAATAGTAACTATGTGTCGTCATGCTTGTTAACACCGTTAATTAAATAAAGATTCCACATTTTTAGTGGATAAAAGGACATGACATAGTAGAAAAAAATGGCTTGAGTGCACATTTGGACAAAAATAGCGTGACATGGCTTATTAAAGGGATTTGATATCTTGAATCAACAGAGAGTACCTTCCTAGTTGTCTAGTTGTGCACTTCTGCCATTTGTCTACCATATGATATCAAAATCAGCCAATGTTTGTCCAATAGGTACGTCAATATCATCATTTAGTTAATCATCTTAACAATAAGAACTATATAGAATGACAATTCAAAGTTTAATGATAACCATAAATTTAGGGACTCAATTTGTAGTTGAACTTTGAAATAGTATGATACTACGACTATGGAGATTCTGGTTTCGACCAATGGATCGGAGAATGTGAAGCCATTTTTAGTCGGACTCTTGCTAAATTTAACATCTATATGTTCTAGTTTTATAGCTGCAAAATTGTTGTACTATTTGTTTTGTATCTCATTTTTTGAATTTTGCAGTCGGAGTTAAGGAAGAGCGAGTTGATCAAAAGAGTTCAAGTCGGGTTTCCGCTTCTGTATCTTTACATGGTGGCGCTGATATGTACTTTTCTGGGACGCCTCTTACGGTAATGAAGAGATTGTTAGTTCTATTCTCTATGAGTTTCTATAATGTTCAAGGTGGCATTAGTATAGCTTGTAGTCCAAAACTTATAGTCGTATAGGTTCCTATTCCCCTTGTAATTGATCGAAGTGTACAATCTAGCAGTCCAGGTAGTGTTATCGCCAACTTGAGCATAGTTTAGTTCGACTTTTCTCTATATTGTAATGTAATGTTGTAAATAATAATAAGCTCCCAAGGGATCTCTTGGTCATATCGGTGAGCGGGCAGGAGAGGTTTCAGTAGGCTCACTTGAAAGTTCGATCAATTTCGCATTGATCAGGTTGGGCGTTCGAATCTCCTCGTAAGCCCTTCAGAAAAAAAGAAAAAGGGGGGGGGGGGGGGGGGGGGGGGNCCTTGCACTCACTCTCGAGTATATGCTTCAACACGAATTAAACAAGGGTATATTATTGATACAATTGAAACCTCTGGCAAAATGTCCTCATGCCCTCTCTATTTTAATGAAAAGGGGGGAATCGCCTGCTAGCTCAAGATCTCAATTGCCTATCCTATGTTCGTCATTCGTTAAACAACTATTTTGTTGGGCGATCAACTAGGTGCCATTGTTGGGTCGATCTGACTGATGGCTCTTCCATCTCTTGTGTATCAAATCGATCGAGAATCTCATCATCTGTTAGGTGGCCACCACTTTTCTACTTTGTGGGAGTCATAATCTACTCTCTTCTAATATGATCGTGACTCTTTCCCCACTTAATTAAACTGTCATCATCCTCCTTAATGATATATACCATGAATACAATCCACTTATTGCACATGGGATCATAACACTATGAATACGTGGGTATTGTGCGATGGGTGAGAACAGGCAATATGGGCCTACATTAAATTGAGAGTTATAATACTGCTACTTGTTGAGTTGTGATTCATCTACAACATCAGAACGTGTCATCACCATGTCAGCCAATATATTTGTTGAGGGCCACGTCATCAAATCTGACCGCTATGGTGCCATGTCATCTGCTACATCATCAAAAAGTACTATTGATTGGTCCACATGTCCACGTAATCAAGCCACGTTAAGCTTTTCCTTCC

mRNA sequence

GAGCATTCAAATTTTAGTAAATAAACACTAAAATGGCCACTTTTTGAACAAACAATTTTTTAAAATACAAAGACTCTCTCTCTCTCCATCTTTCATTTCCTCGGTCTTGAATTATATAGAGAGTTAATTCCACGAAGAACTTTGGCGCTATTTAGTATGTCCATTCCCTCACCGAGCTTTAATTTGTTCGTCCACAACAGATTCTCTTTCGCGAATCAAAACTCTGTTGGTTGAGAACGAGTGATCCAGTTCACTCACTCACTCCGTTTGCTTCTTCTTCTTCTTTGTTTCACTTTTTGAGTTGAGAATGAGTTCGTATAATTTTGAATTCTGGTGACTGGACGAACTCATTGTTCGATCGCTTTCGTGGTGAAACATTGAAGCAAATTTCTCGTCGATTGACAATTGTTTGATGCAATAGCCGAGTGAATTGTTTCTCAACAGAGTTGAATATGGGTATGAGACTTCTGGAAATTCATCCCCAGGAATTGAAGTTCGTCTTTGAACTAAATAAACATAGTTCGTGCTCAATTCAACTAACAAATAATACGCTTGACCATGTTGCTTTTAAGGTTAAGACCACATCCCCTAAGAATTACTTTGTACGGCCAAAGGTCGGAATCATTGAGCCAAAATCAACCCGTGAATTCACAGTTAGGATGCAAGCACAAGAGATGGCTCCACCTGATATGCTATGCAAAGATAAGTTCTTAATTCAGAGCACAGTAGTTTCTGCTGATACAACTGATGAGGACATAACCTCAAGCTTGTTTGCAAAAGATGTTGGCAAGTACATGGAAGAAAAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCAGCTCCCCAATTGATGAAGCATTAAAGGAGGAGTTTGATGTCGGAGTTTCAGAACCAAATAATCTATGCACAGCAGTAGAGAAGGATCTGGACCGTGAAACTTCAAACAAAAGTGATGAAATGTTCAATGTAGGGGAGCCTGAACCGGCAAGTTTAGCTGACGACTTCATAGAAGGCGATGAGGAGGACCTGGATATGATAAAACAGGTTGAGGAGTTGAGGTTAAAGCTCACTGAACTTGAATTAAAGCTCTGTGAGGCTAAAGTTGCCATTTCAAAGCTGAAGGAGGAGAAGAGAATAAGCATCCAAGAAACCGAATTCTTGCAGCAGAATTTTTCGGAGTTAAGGAAGAGCGAGTTGATCAAAAGAGTTCAAGTCGGGTTTCCGCTTCTGTATCTTTACATGGTGGCGCTGATATGTACTTTTCTGGGACGCCTCTTACGGTAATGAAGAGATTGTTAGTTCTATTCTCTATGAGTTTCTATAATGTTCAAGGTGGCATTAGTATAGCTTGTAGTCCAAAACTTATAGTCGTATAGGTTCCTATTCCCCTTGTAATTGATCGAAGTGTACAATCTAGCAGTCCAGGTAGTGTTATCGCCAACTTGAGCATAGTTTAGTTCGACTTTTCTCTATATTGTAATGTAATGTTGTAAATAATAATAAGCTCCCAAGGGATCTCTTGGTCATATCGGTGAGCGGGCAGGAGAGGTTTCAGTAGGCTCACTTGAAAGTTCGATCAATTTCGCATTGATCAGGTTGGGCGTTCGAATCTCCTCGTAAGCCCTTCAGAAAAAAAGAAAAAGGGGGGGGGGGGGGGGGGGGGGGGNCCTTGCACTCACTCTCGAGTATATGCTTCAACACGAATTAAACAAGGGTATATTATTGATACAATTGAAACCTCTGGCAAAATGTCCTCATGCCCTCTCTATTTTAATGAAAAGGGGGGAATCGCCTGCTAGCTCAAGATCTCAATTGCCTATCCTATGTTCGTCATTCGTTAAACAACTATTTTGTTGGGCGATCAACTAGGTGCCATTGTTGGGTCGATCTGACTGATGGCTCTTCCATCTCTTGTGTATCAAATCGATCGAGAATCTCATCATCTGTTAGGTGGCCACCACTTTTCTACTTTGTGGGAGTCATAATCTACTCTCTTCTAATATGATCGTGACTCTTTCCCCACTTAATTAAACTGTCATCATCCTCCTTAATGATATATACCATGAATACAATCCACTTATTGCACATGGGATCATAACACTATGAATACGTGGGTATTGTGCGATGGGTGAGAACAGGCAATATGGGCCTACATTAAATTGAGAGTTATAATACTGCTACTTGTTGAGTTGTGATTCATCTACAACATCAGAACGTGTCATCACCATGTCAGCCAATATATTTGTTGAGGGCCACGTCATCAAATCTGACCGCTATGGTGCCATGTCATCTGCTACATCATCAAAAAGTACTATTGATTGGTCCACATGTCCACGTAATCAAGCCACGTTAAGCTTTTCCTTCC

Coding sequence (CDS)

ATGGGTATGAGACTTCTGGAAATTCATCCCCAGGAATTGAAGTTCGTCTTTGAACTAAATAAACATAGTTCGTGCTCAATTCAACTAACAAATAATACGCTTGACCATGTTGCTTTTAAGGTTAAGACCACATCCCCTAAGAATTACTTTGTACGGCCAAAGGTCGGAATCATTGAGCCAAAATCAACCCGTGAATTCACAGTTAGGATGCAAGCACAAGAGATGGCTCCACCTGATATGCTATGCAAAGATAAGTTCTTAATTCAGAGCACAGTAGTTTCTGCTGATACAACTGATGAGGACATAACCTCAAGCTTGTTTGCAAAAGATGTTGGCAAGTACATGGAAGAAAAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCAGCTCCCCAATTGATGAAGCATTAAAGGAGGAGTTTGATGTCGGAGTTTCAGAACCAAATAATCTATGCACAGCAGTAGAGAAGGATCTGGACCGTGAAACTTCAAACAAAAGTGATGAAATGTTCAATGTAGGGGAGCCTGAACCGGCAAGTTTAGCTGACGACTTCATAGAAGGCGATGAGGAGGACCTGGATATGATAAAACAGGTTGAGGAGTTGAGGTTAAAGCTCACTGAACTTGAATTAAAGCTCTGTGAGGCTAAAGTTGCCATTTCAAAGCTGAAGGAGGAGAAGAGAATAAGCATCCAAGAAACCGAATTCTTGCAGCAGAATTTTTCGGAGTTAAGGAAGAGCGAGTTGATCAAAAGAGTTCAAGTCGGGTTTCCGCTTCTGTATCTTTACATGGTGGCGCTGATATGTACTTTTCTGGGACGCCTCTTACGGTAA

Protein sequence

MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEPKSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR
Homology
BLAST of MC06g1262 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.9e-46
Identity = 146/385 (37.92%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M M LL+I P+ L+F  +L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KST EFTV MQA +  PPDM+CKDKFLIQST VSA+TTDEDIT+S+F+K  GK++EE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSSSP-----------------------------------IDEALKEEFDVGVS 180
            VTLV PS SP                                     E +KE   VG  
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EPNNLCTAVE---------------------------------------------KDLD- 240
           E      A E                                             KD D 
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----RETSN-----------KSDEMFNVG---EPEP-ASLADDFIEGDEED--------- 277
               + T+N             D+ F  G   + EP  S   D ++  + D         
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of MC06g1262 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.6e-32
Identity = 103/274 (37.59%), Postives = 142/274 (51.82%), Query Frame = 0

Query: 5   LLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEPKSTR 64
           LL + P +L+F FEL K  SCS+ LTN T ++VAFKVKTT+PK Y VRP  G++ P+ST 
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVTL 124
           E  V MQAQ+ AP DM CKDKFL+Q  + S   T +++T  +F+K+ G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSP--SSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPASLAD 184
           V+P    SP+ E  +E      S  +N             S  S E F V          
Sbjct: 142 VAPPRPPSPVHEGSEEGSSPRASVSDN----------GHGSEFSFERFIV---------- 201

Query: 185 DFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQNFSE 244
           D   G +E+                      EA+  I+KL EEK+ +IQ    LQ+   +
Sbjct: 202 DNKAGHQENTS--------------------EARALITKLTEEKQSAIQLNNRLQRELDQ 254

Query: 245 LRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 277
           LR+ E  K    G P +Y+ +V LI   LG +++
Sbjct: 262 LRR-ESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of MC06g1262 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-31
Identity = 98/278 (35.25%), Postives = 142/278 (51.08%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M   LL I P +L+F FEL K  SCS+ L N T ++VAFKVKTT+PK Y VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           +S+ E  V MQAQ+ AP D+ CKDKFL+Q  V S   T +D+T  +F+K+ G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSP--SSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPA 180
            V  V+P    SP+ E  +E                                  G    A
Sbjct: 121 RVVYVAPPRPPSPVREGSEE----------------------------------GSSPRA 180

Query: 181 SLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQ 240
           S++D+   G+  D     +    R+   + +    EA+  ++KL EEK  ++Q    LQQ
Sbjct: 181 SVSDN---GNASDFTAAPRFSADRV---DAQDNSSEARALVTKLTEEKNSAVQLNNRLQQ 237

Query: 241 NFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 277
              +LR+ E  +    G P +Y+ +V LI   LG +++
Sbjct: 241 ELDQLRR-ESKRSKSGGIPFMYVLLVGLIGLILGYIMK 237

BLAST of MC06g1262 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.6e-31
Identity = 105/273 (38.46%), Postives = 141/273 (51.65%), Query Frame = 0

Query: 5   LLEIHPQELKFVFELNKHSSCSIQLTNNTLDH-VAFKVKTTSPKNYFVRPKVGIIEPKST 64
           L+ IHP ELKF FEL K SSCS+QLTN T    VAFKVKTT+P+ Y VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVT 124
              TV MQAQ+ AP DM CKDKFL+Q+ VVS  TT +++ + +F K+ G+ +E+ KL V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LVSPS-SSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPASLAD 184
            +  +  SP+ E          SE  N   A   D+  ++               ASL D
Sbjct: 126 YIPANPPSPVPEG---------SEEGNSPMASLNDIASQS---------------ASLFD 185

Query: 185 DFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQNFSE 244
           D     EE  +                 K  EA   ISKL EEK  + Q+++ L+     
Sbjct: 186 DVSRTFEETSE-----------------KSSEAWSMISKLTEEKTSATQQSQKLRLELEM 236

Query: 245 LRKSELIKRVQVGFPLLYLYMVALICTFLGRLL 276
           LRK E  K+   G  LL + +V L+   +G LL
Sbjct: 246 LRK-ETSKKQSGGHSLLLMLLVGLLGCVIGYLL 236

BLAST of MC06g1262 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 64/127 (50.39%), Postives = 87/127 (68.50%), Query Frame = 0

Query: 4   RLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEPKST 63
           +L+ I P ELKF+FEL K S C +++ N T ++VAFKVKTTSPK YFVRP  G+I+P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  REFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVT 123
               V +QAQ   PPDM CKDKFL+QST+V   T  +++    F KD GK + E KL V+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 LVSPSSS 131
            ++PS++
Sbjct: 128 YITPSTT 134

BLAST of MC06g1262 vs. NCBI nr
Match: XP_022154616.1 (vesicle-associated protein 1-2-like isoform X2 [Momordica charantia])

HSP 1 Score: 516 bits (1329), Expect = 8.94e-184
Identity = 276/288 (95.83%), Postives = 276/288 (95.83%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNV-------- 180
           GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNV        
Sbjct: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVPDNVMEPR 180

Query: 181 ----GEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI 240
               GEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI
Sbjct: 181 DVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI 240

Query: 241 SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR
Sbjct: 241 SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 288

BLAST of MC06g1262 vs. NCBI nr
Match: XP_022154614.1 (vesicle-associated protein 1-2-like isoform X1 [Momordica charantia] >XP_022154615.1 vesicle-associated protein 1-2-like isoform X1 [Momordica charantia])

HSP 1 Score: 512 bits (1319), Expect = 4.31e-182
Identity = 276/298 (92.62%), Postives = 276/298 (92.62%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVG------- 180
           GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVG       
Sbjct: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGDSLPPKS 180

Query: 181 ---------------EPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                          EPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA
Sbjct: 181 MVPDNVMEPRDVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR
Sbjct: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 298

BLAST of MC06g1262 vs. NCBI nr
Match: XP_038878362.1 (vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 384 bits (986), Expect = 1.52e-131
Identity = 211/289 (73.01%), Postives = 245/289 (84.78%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHP+ELKF+FELNKHS CSIQLTNNTLDHVAFKVK TSPK YFVRPKVGII P
Sbjct: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            ST EFTVRMQAQ +APPDMLCKDKFLIQSTVV ADTTD DITS+LFAK+ GKY+EE+KL
Sbjct: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPI----DEALKEE-FDVGVSEPNNLCTAVEKDLDRETSNKSD-------- 180
           GVTLVSPS SP+    DEALKEE   + V+EPNNLC AVE+ +D+E  NK+         
Sbjct: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180

Query: 181 EMFNVGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKR 240
              N  EPEP+ L +DFI+G+EE+L+MIK +EEL+LKL+ELELKLC+A+ AISKLKEEK+
Sbjct: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISIQET+FL++NFSELRK+EL  +VQVGFP+LYLYMVA++CTFLGRLLR
Sbjct: 241 ISIQETKFLKENFSELRKTEL-NKVQVGFPVLYLYMVAIVCTFLGRLLR 288

BLAST of MC06g1262 vs. NCBI nr
Match: XP_022961473.1 (vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 382 bits (980), Expect = 1.54e-130
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHPQELKF+ E NKHS CSIQLTNN LDHVAFKVKTTSP  YFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            STREFTVRMQAQ++A PDMLCKD+FLIQSTVV ADTTDEDITSSLFAKD GKY+EE+KL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN--------- 180
           GVTL+SPS   I +ALKEE  +GV+ PNNL TAVE+ +D+E S++S  M N         
Sbjct: 121 GVTLISPS---ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKH 180

Query: 181 -----------VGE--PEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                      VGE  PEPASLA+D I+GD E+L+MIK +EEL+LKL+ELELKLCEA+ A
Sbjct: 181 MVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEK+ISIQE +FLQ+NFSELRK+EL KRVQVGFP+LYLYMVAL+CTFLGR LR
Sbjct: 241 ISKLKEEKQISIQENKFLQENFSELRKTEL-KRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of MC06g1262 vs. NCBI nr
Match: XP_022961469.1 (vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961470.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961471.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_022961472.1 vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 382 bits (980), Expect = 1.90e-130
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHPQELKF+ E NKHS CSIQLTNN LDHVAFKVKTTSP  YFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            STREFTVRMQAQ++A PDMLCKD+FLIQSTVV ADTTDEDITSSLFAKD GKY+EE+KL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN--------- 180
           GVTL+SPS   I +ALKEE  +GV+ PNNL TAVE+ +D+E S++S  M N         
Sbjct: 121 GVTLISPS---ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKH 180

Query: 181 -----------VGE--PEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                      VGE  PEPASLA+D I+GD E+L+MIK +EEL+LKL+ELELKLCEA+ A
Sbjct: 181 MVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEK+ISIQE +FLQ+NFSELRK+EL KRVQVGFP+LYLYMVAL+CTFLGR LR
Sbjct: 241 ISKLKEEKQISIQENKFLQENFSELRKTEL-KRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of MC06g1262 vs. ExPASy TrEMBL
Match: A0A6J1DP93 (vesicle-associated protein 1-2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021837 PE=4 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 4.33e-184
Identity = 276/288 (95.83%), Postives = 276/288 (95.83%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNV-------- 180
           GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNV        
Sbjct: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVPDNVMEPR 180

Query: 181 ----GEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI 240
               GEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI
Sbjct: 181 DVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRI 240

Query: 241 SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR
Sbjct: 241 SIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 288

BLAST of MC06g1262 vs. ExPASy TrEMBL
Match: A0A6J1DMN1 (vesicle-associated protein 1-2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021837 PE=4 SV=1)

HSP 1 Score: 512 bits (1319), Expect = 2.09e-182
Identity = 276/298 (92.62%), Postives = 276/298 (92.62%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP
Sbjct: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL
Sbjct: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVG------- 180
           GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVG       
Sbjct: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGDSLPPKS 180

Query: 181 ---------------EPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                          EPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA
Sbjct: 181 MVPDNVMEPRDVGKGEPEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR
Sbjct: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 298

BLAST of MC06g1262 vs. ExPASy TrEMBL
Match: A0A6J1HBX8 (vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 382 bits (980), Expect = 7.45e-131
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHPQELKF+ E NKHS CSIQLTNN LDHVAFKVKTTSP  YFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            STREFTVRMQAQ++A PDMLCKD+FLIQSTVV ADTTDEDITSSLFAKD GKY+EE+KL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN--------- 180
           GVTL+SPS   I +ALKEE  +GV+ PNNL TAVE+ +D+E S++S  M N         
Sbjct: 121 GVTLISPS---ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKH 180

Query: 181 -----------VGE--PEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                      VGE  PEPASLA+D I+GD E+L+MIK +EEL+LKL+ELELKLCEA+ A
Sbjct: 181 MVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEK+ISIQE +FLQ+NFSELRK+EL KRVQVGFP+LYLYMVAL+CTFLGR LR
Sbjct: 241 ISKLKEEKQISIQENKFLQENFSELRKTEL-KRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of MC06g1262 vs. ExPASy TrEMBL
Match: A0A6J1HAA2 (vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 382 bits (980), Expect = 9.19e-131
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHPQELKF+ E NKHS CSIQLTNN LDHVAFKVKTTSP  YFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            STREFTVRMQAQ++A PDMLCKD+FLIQSTVV ADTTDEDITSSLFAKD GKY+EE+KL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN--------- 180
           GVTL+SPS   I +ALKEE  +GV+ PNNL TAVE+ +D+E S++S  M N         
Sbjct: 121 GVTLISPS---ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKH 180

Query: 181 -----------VGE--PEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                      VGE  PEPASLA+D I+GD E+L+MIK +EEL+LKL+ELELKLCEA+ A
Sbjct: 181 MVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLCEAQNA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEK+ISIQE +FLQ+NFSELRK+EL KRVQVGFP+LYLYMVAL+CTFLGR LR
Sbjct: 241 ISKLKEEKQISIQENKFLQENFSELRKTEL-KRVQVGFPVLYLYMVALVCTFLGRFLR 294

BLAST of MC06g1262 vs. ExPASy TrEMBL
Match: A0A6J1I0M1 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467905 PE=4 SV=1)

HSP 1 Score: 380 bits (977), Expect = 2.13e-130
Identity = 216/298 (72.48%), Postives = 246/298 (82.55%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M MRLLEIHPQELKF+ E NKHS CSIQLTNN L+HVAFKVKTTSP  YFVRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
            STREFTVRMQAQ++A PDMLCKD+FLIQSTVV ADTTDEDITSSLFAKD GKY+EE+KL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFN--------- 180
           GVTLVSPS   I +ALKEE  +GV+ PNNL TAVE+ +D+E S++S  M N         
Sbjct: 121 GVTLVSPS---ISQALKEEEALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDSLPPKH 180

Query: 181 -----------VGE--PEPASLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVA 240
                      VGE  PEPASLA+D I+GD E+L++IK +EEL+LKL+ELELKLCEA+ A
Sbjct: 181 MVPGNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLCEAQNA 240

Query: 241 ISKLKEEKRISIQETEFLQQNFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 276
           ISKLKEEK+ISIQE +FLQ+NFSELRK+EL KRVQVGFP+LYLYMVAL+CTFLGRLLR
Sbjct: 241 ISKLKEEKQISIQENKFLQENFSELRKTEL-KRVQVGFPVLYLYMVALVCTFLGRLLR 294

BLAST of MC06g1262 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-47
Identity = 146/385 (37.92%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M M LL+I P+ L+F  +L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KST EFTV MQA +  PPDM+CKDKFLIQST VSA+TTDEDIT+S+F+K  GK++EE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSSSP-----------------------------------IDEALKEEFDVGVS 180
            VTLV PS SP                                     E +KE   VG  
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EPNNLCTAVE---------------------------------------------KDLD- 240
           E      A E                                             KD D 
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----RETSN-----------KSDEMFNVG---EPEP-ASLADDFIEGDEED--------- 277
               + T+N             D+ F  G   + EP  S   D ++  + D         
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of MC06g1262 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-47
Identity = 146/385 (37.92%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M M LL+I P+ L+F  +L K +SC +QLTN T  +VAFKVKTTSPK Y VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           KST EFTV MQA +  PPDM+CKDKFLIQST VSA+TTDEDIT+S+F+K  GK++EE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSSSP-----------------------------------IDEALKEEFDVGVS 180
            VTLV PS SP                                     E +KE   VG  
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 EPNNLCTAVE---------------------------------------------KDLD- 240
           E      A E                                             KD D 
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 ----RETSN-----------KSDEMFNVG---EPEP-ASLADDFIEGDEED--------- 277
               + T+N             D+ F  G   + EP  S   D ++  + D         
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of MC06g1262 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-33
Identity = 103/274 (37.59%), Postives = 142/274 (51.82%), Query Frame = 0

Query: 5   LLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEPKSTR 64
           LL + P +L+F FEL K  SCS+ LTN T ++VAFKVKTT+PK Y VRP  G++ P+ST 
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVTL 124
           E  V MQAQ+ AP DM CKDKFL+Q  + S   T +++T  +F+K+ G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSP--SSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPASLAD 184
           V+P    SP+ E  +E      S  +N             S  S E F V          
Sbjct: 142 VAPPRPPSPVHEGSEEGSSPRASVSDN----------GHGSEFSFERFIV---------- 201

Query: 185 DFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQNFSE 244
           D   G +E+                      EA+  I+KL EEK+ +IQ    LQ+   +
Sbjct: 202 DNKAGHQENTS--------------------EARALITKLTEEKQSAIQLNNRLQRELDQ 254

Query: 245 LRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 277
           LR+ E  K    G P +Y+ +V LI   LG +++
Sbjct: 262 LRR-ESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of MC06g1262 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 138.3 bits (347), Expect = 9.5e-33
Identity = 98/278 (35.25%), Postives = 142/278 (51.08%), Query Frame = 0

Query: 1   MGMRLLEIHPQELKFVFELNKHSSCSIQLTNNTLDHVAFKVKTTSPKNYFVRPKVGIIEP 60
           M   LL I P +L+F FEL K  SCS+ L N T ++VAFKVKTT+PK Y VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  KSTREFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKL 120
           +S+ E  V MQAQ+ AP D+ CKDKFL+Q  V S   T +D+T  +F+K+ G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSP--SSSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPA 180
            V  V+P    SP+ E  +E                                  G    A
Sbjct: 121 RVVYVAPPRPPSPVREGSEE----------------------------------GSSPRA 180

Query: 181 SLADDFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQ 240
           S++D+   G+  D     +    R+   + +    EA+  ++KL EEK  ++Q    LQQ
Sbjct: 181 SVSDN---GNASDFTAAPRFSADRV---DAQDNSSEARALVTKLTEEKNSAVQLNNRLQQ 237

Query: 241 NFSELRKSELIKRVQVGFPLLYLYMVALICTFLGRLLR 277
              +LR+ E  +    G P +Y+ +V LI   LG +++
Sbjct: 241 ELDQLRR-ESKRSKSGGIPFMYVLLVGLIGLILGYIMK 237

BLAST of MC06g1262 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 136.0 bits (341), Expect = 4.7e-32
Identity = 105/273 (38.46%), Postives = 141/273 (51.65%), Query Frame = 0

Query: 5   LLEIHPQELKFVFELNKHSSCSIQLTNNTLDH-VAFKVKTTSPKNYFVRPKVGIIEPKST 64
           L+ IHP ELKF FEL K SSCS+QLTN T    VAFKVKTT+P+ Y VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQAQEMAPPDMLCKDKFLIQSTVVSADTTDEDITSSLFAKDVGKYMEEKKLGVT 124
              TV MQAQ+ AP DM CKDKFL+Q+ VVS  TT +++ + +F K+ G+ +E+ KL V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LVSPS-SSPIDEALKEEFDVGVSEPNNLCTAVEKDLDRETSNKSDEMFNVGEPEPASLAD 184
            +  +  SP+ E          SE  N   A   D+  ++               ASL D
Sbjct: 126 YIPANPPSPVPEG---------SEEGNSPMASLNDIASQS---------------ASLFD 185

Query: 185 DFIEGDEEDLDMIKQVEELRLKLTELELKLCEAKVAISKLKEEKRISIQETEFLQQNFSE 244
           D     EE  +                 K  EA   ISKL EEK  + Q+++ L+     
Sbjct: 186 DVSRTFEETSE-----------------KSSEAWSMISKLTEEKTSATQQSQKLRLELEM 236

Query: 245 LRKSELIKRVQVGFPLLYLYMVALICTFLGRLL 276
           LRK E  K+   G  LL + +V L+   +G LL
Sbjct: 246 LRK-ETSKKQSGGHSLLLMLLVGLLGCVIGYLL 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD71.9e-4637.92Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ951.6e-3237.59Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC81.3e-3135.25Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q84WW56.6e-3138.46Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9LVU12.1e-2950.39Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022154616.18.94e-18495.83vesicle-associated protein 1-2-like isoform X2 [Momordica charantia][more]
XP_022154614.14.31e-18292.62vesicle-associated protein 1-2-like isoform X1 [Momordica charantia] >XP_0221546... [more]
XP_038878362.11.52e-13173.01vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida][more]
XP_022961473.11.54e-13072.48vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata][more]
XP_022961469.11.90e-13072.48vesicle-associated protein 2-1-like isoform X2 [Cucurbita moschata] >XP_02296147... [more]
Match NameE-valueIdentityDescription
A0A6J1DP934.33e-18495.83vesicle-associated protein 1-2-like isoform X2 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1DMN12.09e-18292.62vesicle-associated protein 1-2-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1HBX87.45e-13172.48vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HAA29.19e-13172.48vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1I0M12.13e-13072.48vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G08820.11.4e-4737.92vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.21.4e-4737.92vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.11.1e-3337.59vesicle associated protein [more]
AT2G45140.19.5e-3335.25plant VAP homolog 12 [more]
AT4G00170.14.7e-3238.46Plant VAMP (vesicle-associated membrane protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 219..239
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..275
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
e-value: 1.1E-28
score: 99.3
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 28.04488
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..276
e-value: 6.9E-50
score: 168.6
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..275
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 2..129
e-value: 2.9E-39
score: 135.9
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 5..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1262.1MC06g1262.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane