MC06g1220 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1220
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF hand family protein
Location	MC06: 16668349 .. 16668714 (+)
RNA-Seq Expression	MC06g1220
Synteny	MC06g1220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGTGGCTTTTCTCAACGACACAACAGTGATAGATTTTATCAATGATACAAAGATATTTGACAACTGCGTGAAGGAAAGTTTCCAGAAACTCGACATTGACAAAGATGGCATCCTCAATGCAAATGAACTTCTGGCCGGGTTTCGTAGTTCAACGGATCCAGTAGATGATCTTTCTCAGACGGTTTGTCGCAAGTTTAATGTGGAAAAAAGTGGTGGTATCAATGAAAATGAGTTTAAGTCGGTCGTAACGGAGATTCTTCTCGCCATTGCTTATGGGATTGGAAACTTACCTCTCCAAGTTGCACTTCAACAAGATGGCCTCCTCATGAAGGCCGTTGAACATGAAAGGGCCAAAGAAGAA

mRNA sequence

ATGAGTGTGGCTTTTCTCAACGACACAACAGTGATAGATTTTATCAATGATACAAAGATATTTGACAACTGCGTGAAGGAAAGTTTCCAGAAACTCGACATTGACAAAGATGGCATCCTCAATGCAAATGAACTTCTGGCCGGGTTTCGTAGTTCAACGGATCCAGTAGATGATCTTTCTCAGACGGTTTGTCGCAAGTTTAATGTGGAAAAAAGTGGTGGTATCAATGAAAATGAGTTTAAGTCGGTCGTAACGGAGATTCTTCTCGCCATTGCTTATGGGATTGGAAACTTACCTCTCCAAGTTGCACTTCAACAAGATGGCCTCCTCATGAAGGCCGTTGAACATGAAAGGGCCAAAGAAGAA

Coding sequence (CDS)

ATGAGTGTGGCTTTTCTCAACGACACAACAGTGATAGATTTTATCAATGATACAAAGATATTTGACAACTGCGTGAAGGAAAGTTTCCAGAAACTCGACATTGACAAAGATGGCATCCTCAATGCAAATGAACTTCTGGCCGGGTTTCGTAGTTCAACGGATCCAGTAGATGATCTTTCTCAGACGGTTTGTCGCAAGTTTAATGTGGAAAAAAGTGGTGGTATCAATGAAAATGAGTTTAAGTCGGTCGTAACGGAGATTCTTCTCGCCATTGCTTATGGGATTGGAAACTTACCTCTCCAAGTTGCACTTCAACAAGATGGCCTCCTCATGAAGGCCGTTGAACATGAAAGGGCCAAAGAAGAA

Protein sequence

MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAKEE

Homology

[Close]

BLAST of MC06g1220 vs. NCBI nr
Match: XP_022158640.1 (uncharacterized protein LOC111025096 [Momordica charantia])

HSP 1 Score: 238 bits (606), Expect = 1.38e-78
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS 60
           MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS
Sbjct: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS 60

Query: 61  QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK 120
           QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK
Sbjct: 61  QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK 120

Query: 121 EE 122
           EE
Sbjct: 121 EE 122

[Close]

BLAST of MC06g1220 vs. NCBI nr
Match: TYJ95513.1 (calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (446), Expect = 2.53e-54
Identity = 94/128 (73.44%), Postives = 103/128 (80.47%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GF S        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDL + VC KFNVEKSGGI+ENEFKSVV +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDENEFKSVVRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+AKE+
Sbjct: 121 ELEKAKEQ 128

[Close]

BLAST of MC06g1220 vs. NCBI nr
Match: XP_004142569.1 (uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical protein Csa_006967 [Cucumis sativus])

HSP 1 Score: 176 bits (445), Expect = 3.59e-54
Identity = 92/128 (71.88%), Postives = 103/128 (80.47%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GFRS        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDTDNDGFLNMNELRVGFRSHPLEFELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDLS+ VC KF VEKSGGI+E EFKSV+ +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLSEVVCHKFQVEKSGGIDEEEFKSVIRDILLAMAQGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+A+E+
Sbjct: 121 ELEKAREQ 128

[Close]

BLAST of MC06g1220 vs. NCBI nr
Match: XP_008462771.1 (PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 173 bits (439), Expect = 2.94e-53
Identity = 92/128 (71.88%), Postives = 102/128 (79.69%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GF S        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDL + VC KFNVEKSGGI+E EFKSV+ +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+AKE+
Sbjct: 121 ELEKAKEQ 128

[Close]

BLAST of MC06g1220 vs. NCBI nr
Match: KAF9854184.1 (hypothetical protein H0E87_005977 [Populus deltoides])

HSP 1 Score: 111 bits (278), Expect = 9.16e-29
Identity = 57/126 (45.24%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGF---------RS 60
           MS+A +N+TTV  F+ D K F+NCVKE F+ LD+D DG+L+ +EL  GF          S
Sbjct: 1   MSLAVVNETTVTTFVEDMKAFENCVKECFEMLDVDGDGVLSRSELREGFCKLMSLGHESS 60

Query: 61  STDPVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLM 117
             + +D L  T+  +F+ ++SG I+  EFK ++ E++ A+  G GN P+ VAL+ D LLM
Sbjct: 61  KKEKIDHLFDTIFDRFDEDQSGSIDPREFKCLIRELMFAMGRGFGNSPVLVALEIDSLLM 120

[Close]

BLAST of MC06g1220 vs. ExPASy TrEMBL
Match: A0A6J1DWE2 (uncharacterized protein LOC111025096 OS=Momordica charantia OX=3673 GN=LOC111025096 PE=4 SV=1)

HSP 1 Score: 238 bits (606), Expect = 6.71e-79
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS 60
           MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS
Sbjct: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSSTDPVDDLS 60

Query: 61  QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK 120
           QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK
Sbjct: 61  QTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERAK 120

Query: 121 EE 122
           EE
Sbjct: 121 EE 122

[Close]

BLAST of MC06g1220 vs. ExPASy TrEMBL
Match: A0A5D3B8A7 (Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1348G00080 PE=4 SV=1)

HSP 1 Score: 176 bits (446), Expect = 1.22e-54
Identity = 94/128 (73.44%), Postives = 103/128 (80.47%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GF S        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDL + VC KFNVEKSGGI+ENEFKSVV +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDENEFKSVVRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+AKE+
Sbjct: 121 ELEKAKEQ 128

[Close]

BLAST of MC06g1220 vs. ExPASy TrEMBL
Match: A0A0A0LYD8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695380 PE=4 SV=1)

HSP 1 Score: 176 bits (445), Expect = 1.74e-54
Identity = 92/128 (71.88%), Postives = 103/128 (80.47%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GFRS        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDTDNDGFLNMNELRVGFRSHPLEFELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDLS+ VC KF VEKSGGI+E EFKSV+ +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLSEVVCHKFQVEKSGGIDEEEFKSVIRDILLAMAQGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+A+E+
Sbjct: 121 ELEKAREQ 128

[Close]

BLAST of MC06g1220 vs. ExPASy TrEMBL
Match: A0A5A7VAA7 (Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00860 PE=4 SV=1)

HSP 1 Score: 173 bits (439), Expect = 1.42e-53
Identity = 92/128 (71.88%), Postives = 102/128 (79.69%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GF S        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDL + VC KFNVEKSGGI+E EFKSV+ +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+AKE+
Sbjct: 121 ELEKAKEQ 128

[Close]

BLAST of MC06g1220 vs. ExPASy TrEMBL
Match: A0A1S3CI80 (uncharacterized protein LOC103501058 OS=Cucumis melo OX=3656 GN=LOC103501058 PE=4 SV=1)

HSP 1 Score: 173 bits (439), Expect = 1.42e-53
Identity = 92/128 (71.88%), Postives = 102/128 (79.69%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST------D 60
           MSVAFLNDTTV +FINDTKIFD+CVKESF+KLD D DG LN NEL  GF S        D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAV 120
           PVDDL + VC KFNVEKSGGI+E EFKSV+ +ILLA+A GIGN PLQVALQQD  LMKAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHERAKEE 122
           E E+AKE+
Sbjct: 121 ELEKAKEQ 128

[Close]

BLAST of MC06g1220 vs. TAIR 10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 74.3 bits (181), Expect = 7.4e-14
Identity = 45/138 (32.61%), Postives = 75/138 (54.35%), Query Frame = 0


Query: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRS--------- 60
           M V  ++ +TV  F++D + F   V E F  LD++KDG+L+ +EL   F S         
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  -----STDPVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVAL-- 120
                  D + +L  ++  KF+ ++SG ++  EF+S + +I+LAIA G+G+ P+ + L  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

Query: 121 QQDGLLMKAVEHERAKEE 123
             D  L KA + E +K E
Sbjct: 121 DDDNFLKKAADLEASKLE 138

[Close]

BLAST of MC06g1220 vs. TAIR 10
Match: AT1G54530.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.3e-13
Identity = 41/121 (33.88%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 6   LNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGFRSST---------DPV 65
           +   T+I F++DTK F++   + FQ LD+DK+G+L+ +EL  G  +           D  
Sbjct: 6   ITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDET 65

Query: 66  DDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEH 118
           D++   +  +F  +    +    F+ ++ EIL A+A GIGN P+ + +  DGL+MKAV H
Sbjct: 66  DNVYNAIFERFGED----LVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLIMKAVLH 122

[Close]

BLAST of MC06g1220 vs. TAIR 10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.0e-07
Identity = 32/114 (28.07%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 6   LNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGF-----------RSSTD 65
           L+ + +++ + + K+FD  V++ FQ+LD D+DG L+  EL               + ++ 
Sbjct: 15  LDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGTSP 74

Query: 66  PVDDLSQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDG 109
             D +   V  +F       +++ EFK V+++ILL +A G+   P+ V L+ DG
Sbjct: 75  DSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPI-VILRMDG 127

The following BLAST results are available for this feature:

BLAST of MC06g1220 vs.


Match Name	E-value	Identity	Description

BLAST of MC06g1220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158640.1	1.38e-78	100.00	uncharacterized protein LOC111025096 [Momordica charantia]	[more]
TYJ95513.1	2.53e-54	73.44	calcium-binding EF hand family protein [Cucumis melo var. makuwa]	[more]
XP_004142569.1	3.59e-54	71.88	uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical ...	[more]
XP_008462771.1	2.94e-53	71.88	PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 cal...	[more]
KAF9854184.1	9.16e-29	45.24	hypothetical protein H0E87_005977 [Populus deltoides]	[more]

BLAST of MC06g1220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWE2	6.71e-79	100.00	uncharacterized protein LOC111025096 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A5D3B8A7	1.22e-54	73.44	Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LYD8	1.74e-54	71.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695380 PE=4 SV=1	[more]
A0A5A7VAA7	1.42e-53	71.88	Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CI80	1.42e-53	71.88	uncharacterized protein LOC103501058 OS=Cucumis melo OX=3656 GN=LOC103501058 PE=...	[more]

BLAST of MC06g1220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44310.1	7.4e-14	32.61	Calcium-binding EF-hand family protein	[more]
AT1G54530.1	1.3e-13	33.88	Calcium-binding EF hand family protein	[more]
AT4G38810.2	1.0e-07	28.07	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 24..52 e-value: 0.022 score: 23.9 coord: 59..87 e-value: 58.0 score: 3.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 20..55 score: 10.803317
None	No IPR available	GENE3D	1.10.238.10		coord: 4..94 e-value: 1.8E-8 score: 36.4
None	No IPR available	PANTHER	PTHR34574:SF12	CALCIUM-BINDING EF HAND FAMILY PROTEIN	coord: 1..120
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 1..120
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 33..45
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 24..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1220.1	MC06g1220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding