MC06g1164 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1164
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase
LocationMC06: 13530847 .. 13532972 (-)
RNA-Seq ExpressionMC06g1164
SyntenyMC06g1164
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGGTTAAAGAAAAAAAAATCTATTATTGACTAATTTTTATTACGATTAACTATCATTTCGATTCAACGGACTTAAAAATTTAGCTAGAAAAAAGAAAAGAAAAAAAAAATTCCAATCCAGAGCTAAAGAGAATAATTTGGGAAAGAGACCTTAAAACCCTTGTTATAATATTATTGTAGTCACACAAGCCCTAAACTATCACCAGTCGCAGCCGAGCCCGAGCATATCTGCAGCAACGCGCCACGAACGACCTCCCTCCGGCGAACCAGACCCGCGGCGGCGTCATTCTTGCAGCACCCACTGTTCTCCCTTCTACGGCGGCGACACCCTGACCCGTCGGCGCTCGCAAACCCGCAGAAGCAAGAACCCAAGCTGTTTCGGTAAGAACCAATTGGCAACGAACCGCTCGCTTGATTGGTACTGATTCCGTGCTTAATTTTTCTGCCTATATCTGCTGACTGATGGCGTTGGAATCTGCTACTCGTGATTCACTCTGTTTTCTTGTGTATTGTATGGTTCTGCTTGACTAACTTCTTAATTTCACTGACATATGAAACCCTTGTATTGCTTGTACTGTGTTGTTCACCTAATATCCTGAAGGTACTATCTGCATCGATTCCATTTGGTTTTCAACTAATAGTTTCTGGAAGTTCTGATAGGATTTTGTCTTCAACTTCCCCTGCTATTTTTGCAATGGAAAAGTTGTTATATGATCCTGTGAGACAGAAATTAGTGTGTGGAGGGAGATAGAGAACATGAAACTTTTTCTGAGACATTCATTCCAGATAATTGTTTTGATCTTCACTGCTTATTGTCCATTCTTGTTATTTTTCTTCTCTGAATTTTGACAGAAATTGCTATTCTATCTTGATCGTTCTTTATGAACATTTGAGGACCAAGGATGTTGCCAACTGATGACTCGAAAAAGATATCGTTTACCCGTTCCATAAGTGACGGAGATTTAGTCATTGTCTATGAGAGACATGACATCATGAAGGCTGTTAAAGTATGTGAAGGTTCAACACTTCAGAATAGGTTTGGTGTGTTTAAACACTCAGATTGGATTGGGAAGCCATTTGGATCCAAGGTGTTGAGTCACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGCTTCATAATTATGTTCTTAGAAATAGTTCCTGGGTGTTTAGTTCTAGAATCAGGGACGGGAAGTGGATCTTTGACAACGTCGCTAGCTAGAGCTGTAGCTCCTACTGGACACGTACATACGTTCGATTTTCATGAACAGAGGGCTGCCTCGGCCAGGTAGGAATGGATATCTATTGATGGTTTTCTTGTCAGATCTTGAGCCAGTTTAAGAAGTAGTACTTATTCCTTGTCTCCCCCTTCTTTCAGGGAGGATTTTGAGAAGACAGGCTTGACTAACTTGGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTCGTTGGGCTGGCCGATTCGGTTTTCTTGGACCTACCTCAACCATGGTTGGCCATTCCTTCAGCTGAGAGGATGCTGAAACCAGATGGGGTATTATGCTCCTTCTCGCCGTGTATCGAGCAAGTTCAGAGGTCGGCAGAAGCTCTTCGATCTAGCTTTACAGGTCCTAAATGCCCCTATGCCATTTTTACCTTCATTGCTAGAGATATGTTGGTAAGTATTGTTCACTCTTTGGTTGTCTCTTTTGTGTAGATATAAGGACGTTTGAGGTATTGCTTCGGACATACGAAGTTCGGGAAGATAAAATGGAGTGTAGCCAGGTTGATGGAGGTGGTTCTGTTGGTCTTCCTCCATGCAAGAGGCGGCTGAGATCAAACCAAGCAAACGACATGTCCGAGGATCCGAGTACAACAGTCATGGTTAAGCCGTGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTTCTAGACTGAGGTGCATCTCATAACATTGATTTTTTTACCAAGGAAAATTGAAGTTTTGTATTCTTTTTCCCTACTCTGGAATGGAATTTTGGATCTCATTTCAGTCGTTGAATGTTAAAGTAATTTTGTTTATGC

mRNA sequence

CGGGTTAAAGAAAAAAAAATCTATTATTGACTAATTTTTATTACGATTAACTATCATTTCGATTCAACGGACTTAAAAATTTAGCTAGAAAAAAGAAAAGAAAAAAAAAATTCCAATCCAGAGCTAAAGAGAATAATTTGGGAAAGAGACCTTAAAACCCTTGTTATAATATTATTGTAGTCACACAAGCCCTAAACTATCACCAGTCGCAGCCGAGCCCGAGCATATCTGCAGCAACGCGCCACGAACGACCTCCCTCCGGCGAACCAGACCCGCGGCGGCGTCATTCTTGCAGCACCCACTGTTCTCCCTTCTACGGCGGCGACACCCTGACCCGTCGGCGCTCGCAAACCCGCAGAAGCAAGAACCCAAGCTGTTTCGAAATTGCTATTCTATCTTGATCGTTCTTTATGAACATTTGAGGACCAAGGATGTTGCCAACTGATGACTCGAAAAAGATATCGTTTACCCGTTCCATAAGTGACGGAGATTTAGTCATTGTCTATGAGAGACATGACATCATGAAGGCTGTTAAAGTATGTGAAGGTTCAACACTTCAGAATAGGTTTGGTGTGTTTAAACACTCAGATTGGATTGGGAAGCCATTTGGATCCAAGGTGTTGAGTCACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGCTTCATAATTATGTTCTTAGAAATAGTTCCTGGGTGTTTAGTTCTAGAATCAGGGACGGGAAGTGGATCTTTGACAACGTCGCTAGCTAGAGCTGTAGCTCCTACTGGACACGTACATACGTTCGATTTTCATGAACAGAGGGCTGCCTCGGCCAGGGAGGATTTTGAGAAGACAGGCTTGACTAACTTGGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTCGTTGGGCTGGCCGATTCGGTTTTCTTGGACCTACCTCAACCATGGTTGGCCATTCCTTCAGCTGAGAGGATGCTGAAACCAGATGGGGTATTATGCTCCTTCTCGCCGTGTATCGAGCAAGTTCAGAGGTCGGCAGAAGCTCTTCGATCTAGCTTTACAGATATAAGGACGTTTGAGGTATTGCTTCGGACATACGAAGTTCGGGAAGATAAAATGGAGTGTAGCCAGGTTGATGGAGGTGGTTCTGTTGGTCTTCCTCCATGCAAGAGGCGGCTGAGATCAAACCAAGCAAACGACATGTCCGAGGATCCGAGTACAACAGTCATGGTTAAGCCGTGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTTCTAGACTGAGGTGCATCTCATAACATTGATTTTTTTACCAAGGAAAATTGAAGTTTTGTATTCTTTTTCCCTACTCTGGAATGGAATTTTGGATCTCATTTCAGTCGTTGAATGTTAAAGTAATTTTGTTTATGC

Coding sequence (CDS)

ATGTTGCCAACTGATGACTCGAAAAAGATATCGTTTACCCGTTCCATAAGTGACGGAGATTTAGTCATTGTCTATGAGAGACATGACATCATGAAGGCTGTTAAAGTATGTGAAGGTTCAACACTTCAGAATAGGTTTGGTGTGTTTAAACACTCAGATTGGATTGGGAAGCCATTTGGATCCAAGGTGTTGAGTCACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCGGACATTAGCTTCATAATTATGTTCTTAGAAATAGTTCCTGGGTGTTTAGTTCTAGAATCAGGGACGGGAAGTGGATCTTTGACAACGTCGCTAGCTAGAGCTGTAGCTCCTACTGGACACGTACATACGTTCGATTTTCATGAACAGAGGGCTGCCTCGGCCAGGGAGGATTTTGAGAAGACAGGCTTGACTAACTTGGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTCGTTGGGCTGGCCGATTCGGTTTTCTTGGACCTACCTCAACCATGGTTGGCCATTCCTTCAGCTGAGAGGATGCTGAAACCAGATGGGGTATTATGCTCCTTCTCGCCGTGTATCGAGCAAGTTCAGAGGTCGGCAGAAGCTCTTCGATCTAGCTTTACAGATATAAGGACGTTTGAGGTATTGCTTCGGACATACGAAGTTCGGGAAGATAAAATGGAGTGTAGCCAGGTTGATGGAGGTGGTTCTGTTGGTCTTCCTCCATGCAAGAGGCGGCTGAGATCAAACCAAGCAAACGACATGTCCGAGGATCCGAGTACAACAGTCATGGTTAAGCCGTGTAGTGAGGCCAGAGGTCACACTGGTTATCTAACATTTTCTAGACTGAGGTGCATCTCATAA

Protein sequence

MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFGSKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLLRTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGYLTFSRLRCIS
Homology
[Close]
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.3e-66
Identity = 141/298 (47.32%), Postives = 188/298 (63.09%), Query Frame = 0 

Query: 10  ISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSHKG 69
           +++   I +GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV+  +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ I M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           +VAPTGH+HT +FH+QRA  ARE+F++  L+  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLKPD-GVLCSFSPCIEQVQRSAEALRS-SFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ +AL +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGYLTFS 305
              +    +              L +N  +D S   S T    P  E  GHTGYLTF+
Sbjct: 244 TVSLPLPDLGA----------NNLETNMGSDASPFRSGT----PMKETVGHTGYLTFA 285

[Close]
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-64
Identity = 141/298 (47.32%), Postives = 184/298 (61.74%), Query Frame = 0 

Query: 10  ISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSHKG 69
           +++   I +GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ I M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH+HT +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLKPD-GVLCSFSPCIEQVQRSAEALRS-SFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ +AL +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 250 EDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGYLTFS 305
                        +V LPP    L +        D S      P  EA GHTGYLTF+
Sbjct: 244 -------------TVSLPP--PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.1e-64
Identity = 138/298 (46.31%), Postives = 186/298 (62.42%), Query Frame = 0 

Query: 10  ISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSHKG 69
           +++   I +GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ I M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           ++APTGH+HT +FH+QRA  ARE+F++  ++  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLKPD-GVLCSFSPCIEQVQRSAEALRS-SFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ +AL +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGYLTFS 305
              +    +              L  N  +D +   S T    P  E  GHTGYLTF+
Sbjct: 244 TVSLPLPDLGADD----------LEGNVGSDATPFRSGT----PMKETVGHTGYLTFA 285

[Close]
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.1e-61
Identity = 139/298 (46.64%), Postives = 184/298 (61.74%), Query Frame = 0 

Query: 10  ISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSHKG 69
           +++   I +GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH+HT +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLKPD-GVLCSFSPCIEQVQRSAEALRS-SFTDIRTFEVLLRTYEVR 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ +AL +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 250 EDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGYLTFS 305
                        +V LP     L +    D +   S T    P  E  GHTGYLTF+
Sbjct: 244 -------------TVSLP--VPDLGARPGPDAAPFRSGT----PMKETVGHTGYLTFA 280

[Close]
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-59
Identity = 130/306 (42.48%), Postives = 185/306 (60.46%), Query Frame = 0 

Query: 16  ISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFGSKVLSHKG-GFVYLL 75
           I +GD V++Y   D M  + +   +   ++FG ++H + IGK +GSK+ S  G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 76  APTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTG 135
           A TPELW++ L HRTQIL+  DIS II  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 136 HVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQG-QGFPEEFVGL-ADSVFLDLPQP 195
           H+ TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 196 WLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALR-SSFTDIRTFEVLLRTYEVREDKME 255
           W AI +A  ++    +LCSFSPCIEQVQ +   L  S F +I+T EVL+RT++ R  + E
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 256 CSQVDGGGSVGLPPCKRRLRSNQANDMSED------------PSTTVMVKPCSEARGHTG 306
              +        P       +N  N++ E+                ++ KP +EARGHTG
Sbjct: 248 ELNLTN------PYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEARGHTG 307

[Close]
BLAST of MC06g1164 vs. NCBI nr
Match: XP_022158112.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia] >XP_022158113.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia])

HSP 1 Score: 626 bits (1615), Expect = 2.03e-226
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTFSRLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of MC06g1164 vs. NCBI nr
Match: XP_038894928.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894929.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894930.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894931.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894932.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894933.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894934.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894935.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894936.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894937.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida])

HSP 1 Score: 570 bits (1470), Expect = 2.56e-204
Identity = 278/310 (89.68%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTD+ KKISFTRSI DGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKH+DWIGKPFG
Sbjct: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLE+VPGCLVLESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLT+LVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEV+E + E +QVDGGGSV LPPCKRRLRS +++DM++D ST VMV+PCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 301 LTFARLRCLS 310

[Close]
BLAST of MC06g1164 vs. NCBI nr
Match: XP_004135565.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sativus] >XP_011659360.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sativus])

HSP 1 Score: 568 bits (1465), Expect = 1.48e-203
Identity = 277/310 (89.35%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTD+ K ISFTRSI DGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SK LS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLT+LVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DG+LCSFSPCIEQVQRS E LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEV+E + E +QVDGGGSVGLPPCKRRLRS +++DM  + STTVMV+PCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 301 LTFARLRCLS 310

[Close]
BLAST of MC06g1164 vs. NCBI nr
Match: KAE8653334.1 (hypothetical protein Csa_023207, partial [Cucumis sativus])

HSP 1 Score: 568 bits (1465), Expect = 1.61e-202
Identity = 277/310 (89.35%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTD+ K ISFTRSI DGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKHSDWIGKPFG
Sbjct: 66  MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 125

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SK LS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 126 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 185

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLT+LVTVGVRDIQGQGFPEEFVG
Sbjct: 186 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 245

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DG+LCSFSPCIEQVQRS E LRS FTDIRTFEVLL
Sbjct: 246 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 305

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEV+E + E +QVDGGGSVGLPPCKRRLRS +++DM  + STTVMV+PCSEARGHTGY
Sbjct: 306 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 365

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 366 LTFARLRCLS 375

[Close]
BLAST of MC06g1164 vs. NCBI nr
Match: XP_022987109.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 565 bits (1456), Expect = 3.36e-202
Identity = 278/310 (89.68%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDD KKISFTRSI DGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLT+LVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVRE K E SQV+GGGSV LPPCK+R+RS +AND + D S+TVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGD-SSTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match: A0A6J1DWC1 (tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024670 PE=3 SV=1)

HSP 1 Score: 626 bits (1615), Expect = 9.81e-227
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTFSRLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 1.63e-202
Identity = 278/310 (89.68%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDD KKISFTRSI DGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLT+LVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVRE K E SQV+GGGSV LPPCK+R+RS +AND + D S+TVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGD-SSTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match: A0A6J1JHY4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 1.63e-202
Identity = 278/310 (89.68%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDD KKISFTRSI DGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLT+LVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVRE K E SQV+GGGSV LPPCK+R+RS +AND + D S+TVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGD-SSTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCIS 310
           LTF+RLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match: A0A6J1J9F9 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 563 bits (1452), Expect = 1.03e-201
Identity = 277/309 (89.64%), Postives = 294/309 (95.15%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDD KKISFTRSI DGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLT+LVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVRE K E SQV+GGGSV LPPCK+R+RS +AND + D S+TVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGD-SSTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCI 309
           LTF+RLRC+
Sbjct: 301 LTFARLRCL 308

[Close]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match: A0A6J1HBB8 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 563 bits (1452), Expect = 1.03e-201
Identity = 277/309 (89.64%), Postives = 294/309 (95.15%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDD KKISFTRSI DGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLT+LVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300
           RTYEVRE K E SQV+GGGSV LPPCK+R+RS +AND + D S+TVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGD-SSTVMVRPCSEARGHTGY 300

Query: 301 LTFSRLRCI 309
           LTF+RLRC+
Sbjct: 301 LTFARLRCL 308

[Close]
BLAST of MC06g1164 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 478.0 bits (1229), Expect = 5.7e-135
Identity = 232/318 (72.96%), Postives = 271/318 (85.22%), Query Frame = 0 

Query: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPT+  K  SF R I DGDLVIVYERHD+MK VKV +   LQNRFGV+KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120
           +KV S+KG FVYLLAPTPELWTLVLSHRTQILYIADISF++M+LE+VPGC+VLESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180
           SL+TSLARAVAPTGHV++FDFHEQRA SAREDFEKTG+++LVTV VRDIQG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240
           LADSVFLDLPQPWLA+PSA +MLK DGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 241 RTYEVREDKMECSQVDGGG----SVGLPPCKRRLRSNQANDMSED----PSTTVMVKPCS 300
           RTYEV+E KM+ S + G      + G+ PCKR+ RSN+ + +S+D     S+ VM +PCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 301 EARGHTGYLTFSRLRCIS 311
           EARGHTGYLTF+RL+C++
Sbjct: 301 EARGHTGYLTFARLQCLA 318

The following BLAST results are available for this feature:
BLAST of MC06g1164 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q80XC29.3e-6647.32tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
Q96FX71.0e-6447.32tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
Q6AY465.1e-6446.31tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
A6H7911.1e-6146.64tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
Q86JJ01.7e-5942.48tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
BLAST of MC06g1164 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022158112.12.03e-226100.00tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica c... [more]
XP_038894928.12.56e-20489.68tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa h... [more]
XP_004135565.11.48e-20389.35tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sat... [more]
KAE8653334.11.61e-20289.35hypothetical protein Csa_023207, partial [Cucumis sativus][more]
XP_022987109.13.36e-20289.68tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform... [more]
BLAST of MC06g1164 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DWC19.81e-227100.00tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1H9F41.63e-20289.68tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JHY41.63e-20289.68tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1J9F91.03e-20189.64tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1HBB81.03e-20189.64tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
BLAST of MC06g1164 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14600.15.7e-13572.96S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..308
e-value: 3.8E-81
score: 273.8
NoneNo IPR availableGENE3D3.10.330.20coord: 10..77
e-value: 7.4E-26
score: 92.0
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 47..251
e-value: 1.6E-43
score: 146.5
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 2..307
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 112..209
e-value: 0.00194655
score: 35.4835
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 69..305
e-value: 1.2E-96
score: 323.2
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PIRSFPIRSF017269GCD14coord: 10..309
e-value: 4.1E-99
score: 328.9
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PANTHERPTHR12133TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASEcoord: 2..307
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROSITEPS51620SAM_TRM61coord: 19..307
score: 99.860229
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 16..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1164.1MC06g1164.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity