MC06g1153 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1153
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	MC06: 13209368 .. 13210336 (+)
RNA-Seq Expression	MC06g1153
Synteny	MC06g1153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACAAAACTTTACTCAATTTGATGCAGATGCTCAAATTGGTGTCTGTTATGGACAACTTGGTGATGACCTACCATCTCCGGTAGAAGTTATCGACCTTTACAATCAGAACAATATCGAAAGAATGCGACTTTACGCTCCCAACCAACCTTCTCTCAACGCTCTTAGAGGCTCCAACATTGAGCTCATGCTCGGCATTCCCAACCACGAGCTTGAAGCCATGGCCACCACTCAAGAAAATGCCAATTCATGGTTTCAAGACAATGTCCTCAGCTTCGGCGATGTGAAATTCAAGTACATTGTCGTCGGAAATGAGATAAAACCGAGCGACGGGGCTGCTAGATTCCTCGTTCCCGCCATGCAAAACATCCAAAACGCAATTTCAGCGGCGGGCTTGAGAGGAAAAATCAAAGTTTCTACTGCCATTGACACCGGAGTTCTTGCAGAGTCCTACCCTCCTTCAAAGGGCTCCTTCAGAAGTGACTATCTTCCGATTCTCGACCCAACCATCCGCTTCCTAGTCGACAACAACTCGCCATTGCTCCTCAACTTGTACACTTATTTCAGTCATATCTCAAACACCAAAGACATTTCTCTCGATTATGCACTTTTTACAGGGATGTCGATGGTTTCAGATGGCCAACTTGTTTATCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGCCGGCGGAGGGTTGGAAATTGTTGTCTCGGAGAGTGGTTGGCCGACGGATGGCGGGGAGGCGGCGAAGGTAGACAATGCAAGAACTTATAATAATAACCTGATTCAACATGTGAAAGGAGGGACTCCAAAGAGACCTGGAATGCCTATTGAAACCTATGTGTTTGCAATGTTTGATGAGAATCAGAAGACGACTCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCCCCAAACAAACAACCTAAATATCTAGTTAACTTC

mRNA sequence

CAACAAAACTTTACTCAATTTGATGCAGATGCTCAAATTGGTGTCTGTTATGGACAACTTGGTGATGACCTACCATCTCCGGTAGAAGTTATCGACCTTTACAATCAGAACAATATCGAAAGAATGCGACTTTACGCTCCCAACCAACCTTCTCTCAACGCTCTTAGAGGCTCCAACATTGAGCTCATGCTCGGCATTCCCAACCACGAGCTTGAAGCCATGGCCACCACTCAAGAAAATGCCAATTCATGGTTTCAAGACAATGTCCTCAGCTTCGGCGATGTGAAATTCAAGTACATTGTCGTCGGAAATGAGATAAAACCGAGCGACGGGGCTGCTAGATTCCTCGTTCCCGCCATGCAAAACATCCAAAACGCAATTTCAGCGGCGGGCTTGAGAGGAAAAATCAAAGTTTCTACTGCCATTGACACCGGAGTTCTTGCAGAGTCCTACCCTCCTTCAAAGGGCTCCTTCAGAAGTGACTATCTTCCGATTCTCGACCCAACCATCCGCTTCCTAGTCGACAACAACTCGCCATTGCTCCTCAACTTGTACACTTATTTCAGTCATATCTCAAACACCAAAGACATTTCTCTCGATTATGCACTTTTTACAGGGATGTCGATGGTTTCAGATGGCCAACTTGTTTATCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGCCGGCGGAGGGTTGGAAATTGTTGTCTCGGAGAGTGGTTGGCCGACGGATGGCGGGGAGGCGGCGAAGGTAGACAATGCAAGAACTTATAATAATAACCTGATTCAACATGTGAAAGGAGGGACTCCAAAGAGACCTGGAATGCCTATTGAAACCTATGTGTTTGCAATGTTTGATGAGAATCAGAAGACGACTCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCCCCAAACAAACAACCTAAATATCTAGTTAACTTC

Coding sequence (CDS)

CAACAAAACTTTACTCAATTTGATGCAGATGCTCAAATTGGTGTCTGTTATGGACAACTTGGTGATGACCTACCATCTCCGGTAGAAGTTATCGACCTTTACAATCAGAACAATATCGAAAGAATGCGACTTTACGCTCCCAACCAACCTTCTCTCAACGCTCTTAGAGGCTCCAACATTGAGCTCATGCTCGGCATTCCCAACCACGAGCTTGAAGCCATGGCCACCACTCAAGAAAATGCCAATTCATGGTTTCAAGACAATGTCCTCAGCTTCGGCGATGTGAAATTCAAGTACATTGTCGTCGGAAATGAGATAAAACCGAGCGACGGGGCTGCTAGATTCCTCGTTCCCGCCATGCAAAACATCCAAAACGCAATTTCAGCGGCGGGCTTGAGAGGAAAAATCAAAGTTTCTACTGCCATTGACACCGGAGTTCTTGCAGAGTCCTACCCTCCTTCAAAGGGCTCCTTCAGAAGTGACTATCTTCCGATTCTCGACCCAACCATCCGCTTCCTAGTCGACAACAACTCGCCATTGCTCCTCAACTTGTACACTTATTTCAGTCATATCTCAAACACCAAAGACATTTCTCTCGATTATGCACTTTTTACAGGGATGTCGATGGTTTCAGATGGCCAACTTGTTTATCAAAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGCCGGCGGAGGGTTGGAAATTGTTGTCTCGGAGAGTGGTTGGCCGACGGATGGCGGGGAGGCGGCGAAGGTAGACAATGCAAGAACTTATAATAATAACCTGATTCAACATGTGAAAGGAGGGACTCCAAAGAGACCTGGAATGCCTATTGAAACCTATGTGTTTGCAATGTTTGATGAGAATCAGAAGACGACTCCTCCTGAGGTTGAGAAACATTGGGGTTTGTTCTCCCCAAACAAACAACCTAAATATCTAGTTAACTTC

Protein sequence

QQNFTQFDADAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSHISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWGLFSPNKQPKYLVNF

Homology

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BLAST of MC06g1153 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 9.3e-117
Identity = 206/320 (64.38%), Postives = 253/320 (79.06%), Query Frame = 0


Query: 5   TQFDADAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELML 64
           T+    A IGVC G +GDDLP   EV+ LY  NNI RMRLY PN  +L ALRGSNI+L+L
Sbjct: 32  TKQHTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLL 91

Query: 65  GIPNHELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQ 124
           G+PN  L+ +A +Q NAN+W Q+NV ++ +VKFKYI VGNE+KPSD  A+FLVPAM+NIQ
Sbjct: 92  GVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQ 151

Query: 125 NAISAAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNL 184
            AIS AGL  KIKVSTAIDTGVL E++PPS GSF+S+Y  +L P IRFLV + SPLL+NL
Sbjct: 152 EAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNL 211

Query: 185 YTYFSHISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIV 244
           Y YF++  NT+DI LDYALFT  S +V DG   Y+NLFDA+LD VY+ALEK+ GG L++V
Sbjct: 212 YPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVV 271

Query: 245 VSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPE 304
           +SE+GWP+  G A  +DNART+ +NLIQHVK GTP+RPG PIETY+FAMFDEN+KT  PE
Sbjct: 272 ISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKT--PE 331

Query: 305 VEKHWGLFSPNKQPKYLVNF 324
           +EKHWGLFSP KQPKY ++F
Sbjct: 332 LEKHWGLFSPTKQPKYQISF 349

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BLAST of MC06g1153 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.5e-114
Identity = 206/318 (64.78%), Postives = 254/318 (79.87%), Query Frame = 0


Query: 10  DAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH 69
           DAQ GVCYG+LG++LP+P EV+ LYNQ NI RMR+Y P+   L ALRGSNIEL+L IPN 
Sbjct: 31  DAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPND 90

Query: 70  ELEAMATTQENANSWFQDNVLSF-GDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAIS 129
            L  +A++Q+NAN W QDN+ ++  +V+F+Y+ VGNE+KP    A+FLVPA++NIQ AIS
Sbjct: 91  NLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAIS 150

Query: 130 AAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYL-PILDPTIRFLVDNNSPLLLNLYTY 189
            AGL  ++KVSTAIDTG LAES+PPSKGSF+SDY    LD  IRFLV+NN+PL++N+Y+Y
Sbjct: 151 NAGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYSY 210

Query: 190 FSHISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSE 249
           F++ +N KDISLDYALF   S +V DG L Y+NLFDA +DAVY+ALEK+ GG L IVVSE
Sbjct: 211 FAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVSE 270

Query: 250 SGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEK 309
           SGWP+ GG A  +DNARTYN NL+++VK GTPKRPG P+ETYVFAMFDENQK   PE EK
Sbjct: 271 SGWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQK--QPEFEK 330

Query: 310 HWGLFSP-NKQPKYLVNF 324
            WGLFSP  KQPKY +NF
Sbjct: 331 FWGLFSPITKQPKYSINF 346

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BLAST of MC06g1153 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.6e-114
Identity = 201/314 (64.01%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 12  QIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHEL 71
           Q+GVCYG+ G++LPSP E I L+ Q NI+R+RLY+P+   L ALRGSNIE+ LG+PN  L
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 72  EAMATTQENANSWFQDNVLSFGD-VKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 131
           +++A++Q  AN+W Q  V+++ + V+F+YI VGNE+K SD  A+FLVPAM+NI  A+ AA
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 132 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 191
           GL G+IKVST++D GVL ESYPPSKGSFR D + +++P IRFLV  NSPLLLNLYTYFS+
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 192 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 251
             N   I LDYALFT  S +VSD    YQNLFDA+LDA+YSALEKS G  LEIVV+E+GW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 252 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 311
           PT GG    ++NAR YNNNLI+HVK GTPKRPG  IETY+FA++DENQK TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 312 LFSPNKQPKYLVNF 324
           LF PNKQPKY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

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BLAST of MC06g1153 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 3.6e-105
Identity = 192/318 (60.38%), Postives = 246/318 (77.36%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQ GVCYG+ G+ LPSP +V+ L N+NNI RMR+Y P+QP+L ALRGSNIELMLG+PN +
Sbjct: 24  AQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTLEALRGSNIELMLGVPNPD 83

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARF---LVPAMQNIQNAI 130
           LE +A +Q NA++W Q+NV ++G+VKF+YI VGNE+ P +  +++   L+ AM+NIQ AI
Sbjct: 84  LENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNENSKYVPVLLNAMRNIQTAI 143

Query: 131 SAAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTY 190
           S AGL  +IKVSTAI+TG+  ++ PPS G F+ D    ++P I FLV N +PLL+NLY Y
Sbjct: 144 SGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPIINFLVTNRAPLLVNLYPY 203

Query: 191 FSHISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSE 250
           F+ I+N  DI L+YALFT    +V+D    Y+NLFDAILDA YSALEK++G  LEIVVSE
Sbjct: 204 FA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYSALEKASGSSLEIVVSE 263

Query: 251 SGWPTDG-GEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVE 310
           SGWP+ G G+   +DNARTYNNNLI HVKGG+PKRP  PIETYVFA+FDE+QK   PE+E
Sbjct: 264 SGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIETYVFALFDEDQK--DPEIE 323

Query: 311 KHWGLFSPNKQPKYLVNF 324
           KH+GLFS N QPKY ++F
Sbjct: 324 KHFGLFSANMQPKYQISF 338

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BLAST of MC06g1153 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 368.6 bits (945), Expect = 7.1e-101
Identity = 185/324 (57.10%), Postives = 239/324 (73.77%), Query Frame = 0


Query: 5   TQFDADAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELML 64
           T+      +GVCYG LG++LP   +V+ LY   NI RMRLY PNQ +L ALRGSNIE+ML
Sbjct: 25  TEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVML 84

Query: 65  GIPNHELEAMATTQENANSWFQDNVLSFGD-VKFKYIVVGNEIKPSDGAA---RFLVPAM 124
           G+PN +L+ +A    NAN+W Q NV +F   VKF+YI VGNE+ P  G +   R+L+PAM
Sbjct: 85  GVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAM 144

Query: 125 QNIQNAISAAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPL 184
           +NI+NAIS+AGL+  IKVS+++D  ++  S+PPS+GSFR+D    +DP I F+   NSPL
Sbjct: 145 RNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSPL 204

Query: 185 LLNLYTYFSHISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGG 244
           L+N+Y YFS+  N +DISL YALFT  + +V DG L Y+NLFDA+ DAVY+AL ++ GG 
Sbjct: 205 LVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGS 264

Query: 245 LEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKT 304
           +EIVVSESGWP+ G  AA  +NA TY  NLIQHVK G+P+RP   IETY+FAMFDEN K 
Sbjct: 265 IEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFDENNKN 324

Query: 305 TPPEVEKHWGLFSPNKQPKYLVNF 324
             PE+EKH+GLFSPNKQPKY ++F
Sbjct: 325 --PELEKHFGLFSPNKQPKYPLSF 346

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BLAST of MC06g1153 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 631 bits (1628), Expect = 1.32e-227
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 10  DAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH 69
           +AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH
Sbjct: 31  NAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH 90

Query: 70  ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA 129
           ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA
Sbjct: 91  ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA 150

Query: 130 AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 189
           AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS
Sbjct: 151 AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 210

Query: 190 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 249
           HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW
Sbjct: 211 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 270

Query: 250 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 309
           PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG
Sbjct: 271 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 330

Query: 310 LFSPNKQPKYLVNF 323
           LFSPNKQPKYLVNF
Sbjct: 331 LFSPNKQPKYLVNF 344

[Close]

BLAST of MC06g1153 vs. NCBI nr
Match: XP_022961079.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 509 bits (1310), Expect = 3.64e-179
Identity = 249/314 (79.30%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYA N P+ +ALRGSNIELMLGIPN+E
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDALRGSNIELMLGIPNNE 90

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK  D AA FLVPAMQNIQNAISA 
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 150

Query: 131 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 190
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 151 GLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 191 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+ G GLEIVVSE+GW
Sbjct: 211 VANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEKAGGDGLEIVVSETGW 270

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 311 LFSPNKQPKYLVNF 323
           LFSP+K PKY VNF
Sbjct: 331 LFSPSKMPKYEVNF 344

[Close]

BLAST of MC06g1153 vs. NCBI nr
Match: XP_022987103.1 (probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima])

HSP 1 Score: 507 bits (1306), Expect = 1.48e-178
Identity = 247/314 (78.66%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYAP+ P+ +ALRGSNIELMLGIPN+E
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 90

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK +D AA FLVPAMQNIQNAISA 
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 150

Query: 131 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 190
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 151 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 191 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+   GLEI VSE+GW
Sbjct: 211 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 270

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 311 LFSPNKQPKYLVNF 323
           LFSP+K PKY VNF
Sbjct: 331 LFSPSKMPKYEVNF 344

[Close]

BLAST of MC06g1153 vs. NCBI nr
Match: KAG6590085.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 507 bits (1305), Expect = 1.13e-177
Identity = 249/314 (79.30%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYAPN P+ +ALRGSNIELMLGIPN+E
Sbjct: 78  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPNHPTFDALRGSNIELMLGIPNNE 137

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           LE +A  QE A+SW QDN++SFGDVKFKYIVVGNEIK  D AA FLVPAMQNIQNAISA 
Sbjct: 138 LEDLANFQEKADSWVQDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 197

Query: 131 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 190
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 198 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 257

Query: 191 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALE S G  +EIVVSE+GW
Sbjct: 258 VANTADISMDYALFRGTSPSVVDGQFVYHNLFDAILDAVYSALETSGGDRVEIVVSETGW 317

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 318 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 377

Query: 311 LFSPNKQPKYLVNF 323
           LFSP+K PKY VNF
Sbjct: 378 LFSPSKLPKYEVNF 391

[Close]

BLAST of MC06g1153 vs. NCBI nr
Match: XP_011659353.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE8653333.1 hypothetical protein Csa_023295 [Cucumis sativus])

HSP 1 Score: 503 bits (1294), Expect = 1.03e-176
Identity = 244/314 (77.71%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSP EVIDLYNQNNI+RMRLYAPNQ + NALRGS+IELMLG+PN +
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPNDQ 92

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           +++MA TQ+NAN+W QDN+L+F DV FKYIVVGNEIK ++ AARFLVPAMQNIQNAISA 
Sbjct: 93  IQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 131 GLRGKIKVSTAIDTGVL-AESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 190
           GL+G+IKVSTA  TG+L AES+PPS GSF ++YLPIL+PTIRFL+DNNSPLLLNLY YFS
Sbjct: 153 GLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFS 212

Query: 191 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           +++ T ++ LDYA+FTG S+V DG+  YQNLFDAILD VYSALEK+ GG LE+VVSE+GW
Sbjct: 213 YVA-TPNMELDYAIFTGTSLVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSETGW 272

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA VDNARTYNNNLIQHVK GTPKR G  IETYVFAMFDEN+KTTPPEVE+HWG
Sbjct: 273 PTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 311 LFSPNKQPKYLVNF 323
           LFSPNKQPKY VNF
Sbjct: 333 LFSPNKQPKYPVNF 345

[Close]

BLAST of MC06g1153 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 631 bits (1628), Expect = 6.39e-228
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 10  DAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH 69
           +AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH
Sbjct: 31  NAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNH 90

Query: 70  ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA 129
           ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA
Sbjct: 91  ELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISA 150

Query: 130 AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 189
           AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS
Sbjct: 151 AGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 210

Query: 190 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 249
           HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW
Sbjct: 211 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 270

Query: 250 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 309
           PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG
Sbjct: 271 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 330

Query: 310 LFSPNKQPKYLVNF 323
           LFSPNKQPKYLVNF
Sbjct: 331 LFSPNKQPKYLVNF 344

[Close]

BLAST of MC06g1153 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 509 bits (1310), Expect = 1.76e-179
Identity = 249/314 (79.30%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYA N P+ +ALRGSNIELMLGIPN+E
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDALRGSNIELMLGIPNNE 90

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK  D AA FLVPAMQNIQNAISA 
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 150

Query: 131 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 190
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 151 GLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 191 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+ G GLEIVVSE+GW
Sbjct: 211 VANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEKAGGDGLEIVVSETGW 270

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 311 LFSPNKQPKYLVNF 323
           LFSP+K PKY VNF
Sbjct: 331 LFSPSKMPKYEVNF 344

[Close]

BLAST of MC06g1153 vs. ExPASy TrEMBL
Match: A0A6J1JIH6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=3 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 7.16e-179
Identity = 247/314 (78.66%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYAP+ P+ +ALRGSNIELMLGIPN+E
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 90

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK +D AA FLVPAMQNIQNAISA 
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 150

Query: 131 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 190
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 151 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 191 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+   GLEI VSE+GW
Sbjct: 211 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 270

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 311 LFSPNKQPKYLVNF 323
           LFSP+K PKY VNF
Sbjct: 331 LFSPSKMPKYEVNF 344

[Close]

BLAST of MC06g1153 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 489 bits (1260), Expect = 7.44e-172
Identity = 241/314 (76.75%), Postives = 274/314 (87.26%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSP EVIDLYNQNNI+RMRLYAPNQ + NALRGS+IELMLG+PN E
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQATFNALRGSSIELMLGLPNDE 92

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           +++MA TQ+NAN+W QDN+L+F DV FKYIVVGNEIK ++ AARFLVPAMQNIQNAISA 
Sbjct: 93  IQSMAATQDNANAWVQDNILNFPDVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 131 GLRGKIKVSTAIDTGVLA-ESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 190
           GL GKIKVSTA  TG+L+ ES+PPS GSF + YLPIL+PTIRFL+DNNSPLL+NLY YFS
Sbjct: 153 GLGGKIKVSTAFHTGILSVESFPPSHGSFDASYLPILNPTIRFLLDNNSPLLVNLYPYFS 212

Query: 191 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           ++  T  + LDYA+FTG S+V DG+  YQNLF AILD VYSALEK+ GG LEIVVSE+GW
Sbjct: 213 YVG-TPSMELDYAIFTGTSLVVDGEFNYQNLFHAILDTVYSALEKNGGGSLEIVVSETGW 272

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PTDGGEAA VDNARTY NNLIQ VK GTPKR G  IETYVFAMFDEN+KTTPPEVE+HWG
Sbjct: 273 PTDGGEAATVDNARTYTNNLIQRVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 311 LFSPNKQPKYLVNF 323
           LFSPNKQPK+ V+F
Sbjct: 333 LFSPNKQPKFSVSF 345

[Close]

BLAST of MC06g1153 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 4.68e-169
Identity = 241/314 (76.75%), Postives = 279/314 (88.85%), Query Frame = 0


Query: 11  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 70
           AQIGVCYGQLG+DLPSP EVIDLYNQNNI+RMRLYAPNQ + NALRGS+IELMLG+PN +
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPNDQ 92

Query: 71  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 130
           +++MA TQ+NAN+W QDN+L+F DV FKYIVVGNEIK ++ AARFLVPAMQNIQNAISA 
Sbjct: 93  IQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 131 GLRGKIKVSTAIDTGVL-AESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFS 190
           GL+G+IKVSTA  TG+L AES+PPS GSF ++YLPIL+PTIRFL+DNNSPLLLNLY YFS
Sbjct: 153 GLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFS 212

Query: 191 HISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 250
           +++ T ++ LDYA+FTG S+V DG+  YQNLFDAILD VYSALEK+ GG LE+VVSE+GW
Sbjct: 213 YVA-TPNMELDYAIFTGTSLVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSETGW 272

Query: 251 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 310
           PT+GGEAA VDNARTYNNNLIQHVK GTPKR G  IETYVFAMFDEN+KTTPPEVE+HWG
Sbjct: 273 PTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 311 LFSPNKQPKYLVNF 323
           LFSPNKQPK  + F
Sbjct: 333 LFSPNKQPKSFLCF 345

[Close]

BLAST of MC06g1153 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 411.4 bits (1056), Expect = 6.8e-115
Identity = 201/314 (64.01%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 12  QIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHEL 71
           Q+GVCYG+ G++LPSP E I L+ Q NI+R+RLY+P+   L ALRGSNIE+ LG+PN  L
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 72  EAMATTQENANSWFQDNVLSFGD-VKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 131
           +++A++Q  AN+W Q  V+++ + V+F+YI VGNE+K SD  A+FLVPAM+NI  A+ AA
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 132 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 191
           GL G+IKVST++D GVL ESYPPSKGSFR D + +++P IRFLV  NSPLLLNLYTYFS+
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 192 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 251
             N   I LDYALFT  S +VSD    YQNLFDA+LDA+YSALEKS G  LEIVV+E+GW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 252 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 311
           PT GG    ++NAR YNNNLI+HVK GTPKRPG  IETY+FA++DENQK TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 312 LFSPNKQPKYLVNF 324
           LF PNKQPKY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

[Close]

BLAST of MC06g1153 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 366.3 bits (939), Expect = 2.5e-101
Identity = 188/314 (59.87%), Postives = 235/314 (74.84%), Query Frame = 0


Query: 12  QIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHEL 71
           QIGVCYG LGD LPSP +V+ LY Q NI+RMRLY P+  +L ALRGS+IEL+L +P+ +L
Sbjct: 31  QIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDL 90

Query: 72  EAMATTQENANSWFQDNVLSFGD-VKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 131
           E +A++Q  A+ W Q+NV S+ D V+F+YI VGNE+KPS G   FL+ AMQNI+NA+S A
Sbjct: 91  ERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPSVGG--FLLQAMQNIENAVSGA 150

Query: 132 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 191
           GL  ++KVSTAI T    ++ PPS+G FR +Y   L+P I FL    SPLL+NLY YFS+
Sbjct: 151 GL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 210

Query: 192 ISNTKDISLDYALFTGMSMV-SDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 251
           + +T +I LDYALFT  S V +D    YQNLFDA LD+VY+ALEKS GG LEIVVSE+GW
Sbjct: 211 MGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETGW 270

Query: 252 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 311
           PT+G     V+NA+TY NNLIQHVK G+P+RPG  IETY+FAMFDEN+K   P  EK WG
Sbjct: 271 PTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKK--EPTYEKFWG 330

Query: 312 LFSPNKQPKYLVNF 324
           LF P++Q KY VNF
Sbjct: 331 LFHPDRQSKYEVNF 338

[Close]

BLAST of MC06g1153 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 364.8 bits (935), Expect = 7.3e-101
Identity = 187/316 (59.18%), Postives = 234/316 (74.05%), Query Frame = 0


Query: 9   ADAQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPN 68
           A  QIGVCYG+ G++L    EV+ LY Q NI RMRLY PNQ +LNALRGSNIEL+L +PN
Sbjct: 31  AVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPN 90

Query: 69  HELEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAIS 128
            +L+ +A++Q  A++W ++NV ++ +V F+YI VGNE++PSD AA F++PAMQNI+ A+S
Sbjct: 91  PDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVS 150

Query: 129 AAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYF 188
           + G    IKVSTAIDT  ++  +PPS G+F  ++   + P I FL    SPLL+N Y YF
Sbjct: 151 SLG----IKVSTAIDTRGIS-GFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYF 210

Query: 189 SHISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSES 248
           S+  N +DI LDY LFT  S +V+DGQ  Y+NLF AILD VY++LEK+ GG LEIVVSES
Sbjct: 211 SYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSES 270

Query: 249 GWPTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKH 308
           GWPT GG A  VDNARTY NNLIQ VK G+P+RPG   ETY+FAMFDEN K   PE EK 
Sbjct: 271 GWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSK-QGPETEKF 330

Query: 309 WGLFSPNKQPKYLVNF 324
           WGLF PN QPKY+VNF
Sbjct: 331 WGLFLPNLQPKYVVNF 340

[Close]

BLAST of MC06g1153 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 7.8e-95
Identity = 174/315 (55.24%), Postives = 229/315 (72.70%), Query Frame = 0


Query: 13  IGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHELE 72
           +GVCYG +G++LPS  + I L+ QNNI R+RLY PNQ +LNALR + IE+++G+PN +L 
Sbjct: 24  VGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVPNTDLR 83

Query: 73  AMATTQENANSWFQDNVLS-FGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAAG 132
           ++ T   +A SW Q+NVL+ +  V FKYI VGNE+ PS+G    ++PAM+N+ +A+  A 
Sbjct: 84  SL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDALRGAN 143

Query: 133 LRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSHI 192
           L+ +IKVSTAID  ++  S+PPS G FR D    +DP I FL   NS LL N+Y YFS++
Sbjct: 144 LQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYFSYV 203

Query: 193 SNTKDISLDYALFTGMSMVS-DGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGWP 252
            N +DISL YALFT  S+V  DG   YQNLFDA+LD VYSA+E+S GG L +VVSESGWP
Sbjct: 204 DNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSESGWP 263

Query: 253 TDGGEAAKVDNARTYNNNLIQHVK--GGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHW 312
           ++GG AA  DNAR +  NL   V+   GTPKRPG  +ETY+FAMFDENQK+  PE+EK++
Sbjct: 264 SNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKS--PEIEKNF 323

Query: 313 GLFSPNKQPKYLVNF 324
           GLF PNKQPK+ + F
Sbjct: 324 GLFFPNKQPKFPITF 334

[Close]

BLAST of MC06g1153 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.0e-70
Identity = 136/323 (42.11%), Postives = 207/323 (64.09%), Query Frame = 0


Query: 12  QIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHEL 71
           ++GVCYG+  DDLP+P +V+ L  Q+NI+ +R+Y  N   L A   ++IELM+G+PN +L
Sbjct: 25  KVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDL 84

Query: 72  EAMATTQENANSWFQDNVLS-FGDVKFKYIVVGNEI--KPSDGAARFLVPAMQNIQNAIS 131
            A + +Q N ++W +++VL  +   K  YI VG E    P   A+ F+VPAMQN+  A+ 
Sbjct: 85  NAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALR 144

Query: 132 AAGLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYF 191
             GL  +IKVST +  G+L+ S+PPS G+F S Y   L P + FL +N SP +++LY Y+
Sbjct: 145 KVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYY 204

Query: 192 SHISNTKDISLDYALFTGMSMVSDGQ--LVYQNLFDAILDAVYSALEKSAGGGLEIVVSE 251
           ++  +  ++SLDY LF   S V D    L+Y+N+FDA +DA+Y AL       ++I+V+E
Sbjct: 205 AYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVTE 264

Query: 252 SGWPTDGG----EAAKVDNARTYNNNLIQHV--KGGTPKRPGMPIETYVFAMFDENQKTT 311
           +GWPT G      AA  DNA TYN+N+I+HV    GTP +PG  +  Y+F++F+EN+K  
Sbjct: 265 TGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRK-A 324

Query: 312 PPEVEKHWGLFSPNKQPKYLVNF 324
             + E++WGLF P++   Y ++F
Sbjct: 325 GLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of MC06g1153 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	9.3e-117	64.38	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
Q03773	2.5e-114	64.78	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
Q9M2M0	9.6e-114	64.01	Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P36401	3.6e-105	60.38	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]
P07979	7.1e-101	57.10	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]

BLAST of MC06g1153 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139185.1	1.32e-227	99.68	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]
XP_022961079.1	3.64e-179	79.30	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata]	[more]
XP_022987103.1	1.48e-178	78.66	probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima]	[more]
KAG6590085.1	1.13e-177	79.30	Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma...	[more]
XP_011659353.1	1.03e-176	77.71	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE86533...	[more]

BLAST of MC06g1153 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBL7	6.39e-228	99.68	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ...	[more]
A0A6J1HCY3	1.76e-179	79.30	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P...	[more]
A0A6J1JIH6	7.16e-179	78.66	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=...	[more]
A0A1S3C9Z3	7.44e-172	76.75	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV...	[more]
A0A0A0LYQ8	4.68e-169	76.75	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3...	[more]

BLAST of MC06g1153 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57270.1	6.8e-115	64.01	beta-1,3-glucanase 1	[more]
AT3G57260.1	2.5e-101	59.87	beta-1,3-glucanase 2	[more]
AT3G57240.1	7.3e-101	59.18	beta-1,3-glucanase 3	[more]
AT4G16260.1	7.8e-95	55.24	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	3.0e-70	42.11	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 11..323 e-value: 3.2E-131 score: 439.1
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 10..320
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 13..323 e-value: 3.0E-119 score: 398.2
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 240..253
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 10..320
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 13..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1153.1	MC06g1153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds