MC06g1136 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1136
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	seipin-1
Location	MC06: 12537031 .. 12541196 (+)
RNA-Seq Expression	MC06g1136
Synteny	MC06g1136

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGACGACGAACACGAAGAATCCGGCCTCCCAATCCCAGCGCCCACCGATTTCTTCAACAAACTAATATTCCTCGCCGCCGAATTAATCTACGCCGCCATCGCCTTCGTCCTCGCCCCGATTTCCACTCTCCTCTCTCTGCTCTCCGAGTCCTTCCTCCACGCAGAGGAGGCGAAGCAGAGCGTCGAATCCGCCGTCCGGAGAGCCCCCTCCGACGCCGCCCGACGCGTCCGCCTCGCCGCGAGGAGGGTGAGCTACGGGCTGGCGGCGGCGGCACTGGTGTGCGTGGCGATGGTGGTGCTTCTGGCGGCGGCGGCGGGGGCGAGCGCGGCGGCGATGAGGGCGTGGATGGAGGAGCCGGTGATGGTGAGGGAGAGATTGAACTTCGATTACACGCAGGCGCAGCCTAGGGCTGTGTTCGGGGCGGAGAATAATGGGAAGAAGAACAATTTGGGGATTCCGGTGGGGCACCGGTTTACGGCGTCGGTGATTCTTCTGATGCCGGAATCTGATTTCAATCGCCATGTTGGTGTTTTTCAGGTACTTTTTGCTGTTCATCTTAATGGAGTGATTTGAGTTCATGTTCATTGTTTTTGTTCGTTAAGTGCAATATATTGGGCAGAGGATTCGAGCTGATTAATTCTTTTTGTTGTATTCACATGTCAAAAGCCAAAAATAGTATACTTTTAGTAGAGTTAGTTTGGACCAACAACTAGCTTTGACTGAAAGTTTATTTTAATGCTAGAAAGCTCTAGAGATATAGTTGAGAATTGCTTGTGGCTTATTTATTAGACATAAATTCAATTTTGTGGATAGCGCACTGGATGTTAAAAATTATGGAGAAGGTACTAGGGCTGGGTAAGGGGGGCATTTGGGTCGCGGGTTTGGGTTCGAGTATTTTTTATCTTTTTTTCTAAATTTCTTTCTTAACTATTACACCCTTTTACAATATTTGATTTTTCTAAATTAAAAAAGAAGAAAAAAAATGGTGGGTAACTCTATTATTTTGAAATTTTGCCAATTTAAGACTTTGAGGGATGCATTTGTATTATATTAGTGTTTAGTAATGCTTTGACGAATTGAATTTTGTTCCCATAATGCCAACGTTTAGCATGTTGTTGCTTTTGCAATGGTGGTTTGAATTTAAGAAGTGTTATATTTCATTAGATGTATTTTTTGAATGTTTAATATATATATTTAATAATATTTCTAAACTTGGAAATTTACTATAACAAAATGAGGGGAAATGTGTAGAGAGAGATTAATAAGAGAAAACATCTGTAAGAAAGATTACTAAGAGACTAAGGGCATGTTTGCCGGCCCTCCCAAAATCACTTTTAAAATCACTCTCATTTTAAAACAGAATGTTTTGGAAAGATTCATTCTCCAAAATCACTTTGATGATTTCAATTGGAGGGTGATTCTCCAAGTGATTTTTGATAAAAAAGAAATAATATATATATATATATATATAATTAGTTTTTCAAATAGTTATTGATTTAATTTTCAAGATTGTATTGCTTTTCACCTCATTTTCACTTTAAATTTTGAGAAATTTTTTTTTTCCCTCTTCTACCAATTTTGTCCCTTTAACTTTATCATTTTGTTTATTCCCTCTAATTTTGTTCTCTAAAATTTTCGTAAACTCTCCCTAAATTTTTAGTTCCCATCTTTCTAAGTTTATACTATCTAAAATTTTTGTCTCTCACACTTTCAAATTTTTCTCTCCAAGATTTTTTCTCTCTTTAAAAGTTTTGTCAAATTCTCTCTTTAAACTTTAATACTAATCATTCAACATCATTTTTAATTATATAAAAATCACTTGAAAAAATGTGTAACCAAATGTTAAAATAATTATATAAAAATCACTTTCACAAAGTGAATTATCATTAAATTTCTTTTGTTTACTTATTCTAAAGTGATTCTCAAATAGGTCCGACAAAAATTGACAAGAAGGCTCATGTAGGTGTAATAAGAAGTTAGTAGAAGAAAATTTGGGTGAGAAGGGAGAATTCAAAAATTAAGGTGTAGATGAATTAGTGAGAGAAACTTATGGAGAGAGATATTAGGAGGGAAAAGACAAGTAAAGATATTGGTAACAAAAGGAAAAAAAAAAAGTGTTAGAAAGAAGTTGATATAGGGAGAATAATTAGGGAAAAAAGTGTCCTTGTAGATTAAGGACCATGCATTAAGGATAAAAAAGCACTTAATTGTTTATTTGAAAAATTATTTAAAAGTCTTCTTTTGCTTCTTTTTTTTTTTAAGTGGTTTTAAATCATAGTTAAATATGTATGATTTGAGGTTGGAATAGTTCAAAAGACTATTGCACACAAAATTGAAATTTCAAAGGCTTCGCAAAACTTAAAATTAATTGAAAGGAACATATTAGACACAAATTTCAAATCTGAAAGTTTAGAGACTAAATTTATTTATTATTATTATTTTTAAGTACAACATGTTGGGGTTGGGTTCGAACTTACAACCTCATGATTGGAGGAATGTGTTAATTATCGTGCTTACTTTGACACTTATAATTTAATTTAATAACGACCTTTTGCATTCTTATCATTATTATATATTCCGCAAATAGGGTTTATCTTGCCCATGAACAATCCTAGCTAATTCATGCAATATTGAATTCTTTTTCCTTGTGGAAAACATGTAACAATTGAAAACATAAAAAAGTAAATCTAAAAATTCAAGAAGTAAGATTGGATCTAAAAATTTAAAAATAAATCCCAAATCATGCCTAGGATGCCTATGCCTGTGTTTGAATCATTTCTATTTGTGGCTTTTCACAAATTTTCAAGTCCACATGCAAGAGAGATGTCTTTCCGCAAATTTTCAAGAGAGTATTGGATATCCTATAAATTATTTAAATTTAATTATATTTAACAATGCTAAGTGAAAGCTAATAAATTTTAATACAAAATTTGGAAGTTAAAATATAATATTTCAAATATTAAAAAAAGTTCATAACCATGCTCAAACGTCAAACCCAAGAGATTTACATTCTTTAGGTGACATTTCATATATGAACTTCTAAAACTCACCATTTCCTAATTCAGTTGCGTGCAGAGCTGATATCAACAAATGGAAACATAATAACCAGTTCAAGCTTGCCCTGCATGCTCCGGTTCAGAAGCAGGCCGGTCCGGTTGACGCGAACATTTCTCGCTAGCATCCCTTTAGTCTTAGGAATCTCGACCGAATCCCAAGAGCTAACCTTTCACATGCTAAAGCACAAGGAAGTAAATCATCAACGAAGCAAAGCTATCCGAGTTACTATTATTCCTCGTGTTGGGACCTTAGCTCTCCCGGAGCTTTACGAAGCTCGAATTCACATAAACTCGCAGCTACCGCCGATGAAACAGCTGCTACATCGCTGGCGATGGACTTGCTACCTATGGACAACTTTTTATACGTACCTAGTGTTTTTGGTGATGTTTATGTACTTTTGGAAGCCCGTTGTGTTTCGGGCAGCATCATTGAAGGAGTTGAGTGGTAACAATCGGGGAGCTTCGGCGAGTAGGGAAGCAGAGGAGTCGTTCGACGATATGGCAGATATAACGGTGGAGTTGTTGAGGAAATGGCAGGAGATGAGGAGGAAAAGGAAGGCTGCAATGTTTGCGTATAGAGAAGTTGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGACGGAGAAGGCGATGTTGGGTATGGAGAAGTTGAAGAAGAAGACGTTGGGTCGACATCGGCATCGAGCATCAGTTGTAGTAGAGATTATACAGTTTCAGGTTTTGAGGAAGATGTCGGGGACTCAGAG

mRNA sequence

ATGGATTCCGACGACGAACACGAAGAATCCGGCCTCCCAATCCCAGCGCCCACCGATTTCTTCAACAAACTAATATTCCTCGCCGCCGAATTAATCTACGCCGCCATCGCCTTCGTCCTCGCCCCGATTTCCACTCTCCTCTCTCTGCTCTCCGAGTCCTTCCTCCACGCAGAGGAGGCGAAGCAGAGCGTCGAATCCGCCGTCCGGAGAGCCCCCTCCGACGCCGCCCGACGCGTCCGCCTCGCCGCGAGGAGGGTGAGCTACGGGCTGGCGGCGGCGGCACTGGTGTGCGTGGCGATGGTGGTGCTTCTGGCGGCGGCGGCGGGGGCGAGCGCGGCGGCGATGAGGGCGTGGATGGAGGAGCCGGTGATGGTGAGGGAGAGATTGAACTTCGATTACACGCAGGCGCAGCCTAGGGCTGTGTTCGGGGCGGAGAATAATGGGAAGAAGAACAATTTGGGGATTCCGGTGGGGCACCGGTTTACGGCGTCGGTGATTCTTCTGATGCCGGAATCTGATTTCAATCGCCATGTTGGTGTTTTTCAGTTGCGTGCAGAGCTGATATCAACAAATGGAAACATAATAACCAGTTCAAGCTTGCCCTGCATGCTCCGGTTCAGAAGCAGGCCGGTCCGGTTGACGCGAACATTTCTCGCTAGCATCCCTTTAGTCTTAGGAATCTCGACCGAATCCCAAGAGCTAACCTTTCACATGCTAAAGCACAAGGAAGTAAATCATCAACGAAGCAAAGCTATCCGAGTTACTATTATTCCTCGTGTTGGGACCTTAGCTCTCCCGGAGCTTTACGAAGCTCGAATTCACATAAACTCGCAGCTACCGCCGATGAAACAGCTGCTACATCGCTGGCGATGGACTTGCTACCTATGGACAACTTTTTATACGTACCTAGTGTTTTTGGTGATGTTTATGTACTTTTGGAAGCCCGTTGTGTTTCGGGCAGCATCATTGAAGGAGTTGAGTGGTAACAATCGGGGAGCTTCGGCGAGTAGGGAAGCAGAGGAGTCGTTCGACGATATGGCAGATATAACGGTGGAGTTGTTGAGGAAATGGCAGGAGATGAGGAGGAAAAGGAAGGCTGCAATGTTTGCGTATGAAGAAGAAGACGTTGGGTCGACATCGGCATCGAGCATCAGTTGTAGTAGAGATTATACAGTTTCAGGTTTTGAGGAAGATGTCGGGGACTCAGAG

Coding sequence (CDS)

ATGGATTCCGACGACGAACACGAAGAATCCGGCCTCCCAATCCCAGCGCCCACCGATTTCTTCAACAAACTAATATTCCTCGCCGCCGAATTAATCTACGCCGCCATCGCCTTCGTCCTCGCCCCGATTTCCACTCTCCTCTCTCTGCTCTCCGAGTCCTTCCTCCACGCAGAGGAGGCGAAGCAGAGCGTCGAATCCGCCGTCCGGAGAGCCCCCTCCGACGCCGCCCGACGCGTCCGCCTCGCCGCGAGGAGGGTGAGCTACGGGCTGGCGGCGGCGGCACTGGTGTGCGTGGCGATGGTGGTGCTTCTGGCGGCGGCGGCGGGGGCGAGCGCGGCGGCGATGAGGGCGTGGATGGAGGAGCCGGTGATGGTGAGGGAGAGATTGAACTTCGATTACACGCAGGCGCAGCCTAGGGCTGTGTTCGGGGCGGAGAATAATGGGAAGAAGAACAATTTGGGGATTCCGGTGGGGCACCGGTTTACGGCGTCGGTGATTCTTCTGATGCCGGAATCTGATTTCAATCGCCATGTTGGTGTTTTTCAGTTGCGTGCAGAGCTGATATCAACAAATGGAAACATAATAACCAGTTCAAGCTTGCCCTGCATGCTCCGGTTCAGAAGCAGGCCGGTCCGGTTGACGCGAACATTTCTCGCTAGCATCCCTTTAGTCTTAGGAATCTCGACCGAATCCCAAGAGCTAACCTTTCACATGCTAAAGCACAAGGAAGTAAATCATCAACGAAGCAAAGCTATCCGAGTTACTATTATTCCTCGTGTTGGGACCTTAGCTCTCCCGGAGCTTTACGAAGCTCGAATTCACATAAACTCGCAGCTACCGCCGATGAAACAGCTGCTACATCGCTGGCGATGGACTTGCTACCTATGGACAACTTTTTATACGTACCTAGTGTTTTTGGTGATGTTTATGTACTTTTGGAAGCCCGTTGTGTTTCGGGCAGCATCATTGAAGGAGTTGAGTGGTAACAATCGGGGAGCTTCGGCGAGTAGGGAAGCAGAGGAGTCGTTCGACGATATGGCAGATATAACGGTGGAGTTGTTGAGGAAATGGCAGGAGATGAGGAGGAAAAGGAAGGCTGCAATGTTTGCGTATGAAGAAGAAGACGTTGGGTCGACATCGGCATCGAGCATCAGTTGTAGTAGAGATTATACAGTTTCAGGTTTTGAGGAAGATGTCGGGGACTCAGAG

Protein sequence

MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEAKQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE

Homology

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BLAST of MC06g1136 vs. ExPASy Swiss-Prot
Match: Q9FFD9 (Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.2e-58
Identity = 138/363 (38.02%), Postives = 209/363 (57.58%), Query Frame = 0


Query: 5   DEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEAKQSV 64
           ++  +  +P P   D+F  L+ + A+LIY A+  + +P      LL  S+  A     + 
Sbjct: 11  EQDNQLKIPEPLRADWFMVLVTIQADLIYNALVVLSSP----FFLLYRSYRRAVVTVSAA 70

Query: 65  ESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVM 124
           E AV+RAP+  A       RR  +G+  A  V + MV+ L  A       +  ++E+PV+
Sbjct: 71  EKAVKRAPAQIAGGAGRVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVV 130

Query: 125 VRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGVFQLR 184
           VR+RL FDYT+  P AVF  +   KK +  +PVGH    S++L MPES+ NR +GVFQL+
Sbjct: 131 VRDRLFFDYTEENPSAVFSFDK--KKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQLK 190

Query: 185 AELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLKHKEV 244
            EL+S  G  I  SS PCMLRFRS+P+RL RTF+ S+PL+ GI+ E+Q +    LKH+E 
Sbjct: 191 VELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKHQE- 250

Query: 245 NHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYLV 304
              R+KA+R T+IPR  T  LP+LYEA I INS+ P +K++ + W+WT  +WT+ Y Y+ 
Sbjct: 251 KMPRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYLYVA 310

Query: 305 FLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEMRRKR 364
            L   ++ ++PV+F   S + +S      S + E E   ++   +     R+ +   R+R
Sbjct: 311 ILTALLWCFRPVLFPYTSSRTIS-----ESENLEIEVVEEEQEQVMERRRRERRNQPRRR 361

Query: 365 KAA 368
             A
Sbjct: 371 NFA 361

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BLAST of MC06g1136 vs. ExPASy Swiss-Prot
Match: Q8L615 (Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-26
Identity = 77/252 (30.56%), Postives = 137/252 (54.37%), Query Frame = 0


Query: 84  RRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVMVRERLNFDYTQAQPRA--- 143
           RRV++GL  A  V + +  LL +A   S   +     EP++++E LNFDYT++ P A   
Sbjct: 232 RRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSSPEAYVP 291

Query: 144 -------VFGAE-----NNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGVFQLRAELI 203
                   FG         GK   L      R   +V + +PES++NR++G+FQ+R + +
Sbjct: 292 ISSCAGVAFGLSGKESIETGKVKGL----KDRTEITVSMTLPESEYNRNLGMFQVRVDFL 351

Query: 204 STNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLKHKEVNHQR 263
           S +G+++ SS  PCM++F S P+RL +T L   PLV G  +E Q L   +    E +   
Sbjct: 352 SASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGLVEKDIIP 411

Query: 264 SKAIRVTIIPRV---GTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYLVF 318
           +  +++ I  R        +PE+Y+A + + S+LP +K+++  WR T ++W +   +++ 
Sbjct: 412 TACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWISMSLFIME 471

[Close]

BLAST of MC06g1136 vs. ExPASy Swiss-Prot
Match: F4I340 (Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 8.2e-22
Identity = 76/267 (28.46%), Postives = 133/267 (49.81%), Query Frame = 0


Query: 85  RVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVMVRERLNFDYTQAQPRAVF-- 144
           +  +G+  A  V + +  LL ++       +    ++P  V+E LNFDYT+  P A    
Sbjct: 257 KFGWGMFWAVYVGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPI 316

Query: 145 ----GAENNG---KKNNLG-------IPVGHRFTASVILLMPESDFNRHVGVFQLRAELI 204
               G E  G   + N +        IP   +    + + +PES +N+++G+FQ+R + +
Sbjct: 317 SSCAGVECEGSCKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQVRVDFL 376

Query: 205 STNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHM--LKHKEVNH 264
           S +G  I S   PCMLRFRS P+RL +TF   +PLV G  +E Q L+  +     K++  
Sbjct: 377 SVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPT 436

Query: 265 QRSKAI---RVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYL 324
              K I   R    P  G   +PELY+A + + S LP  ++++ +WR T ++W +   ++
Sbjct: 437 ACLKIIIEQRAEFRPGAG---IPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFI 496

Query: 325 VFLVMFMYFWKPVVFRAASLKELSGNN 331
             L+  +   +P++      ++ S +N
Sbjct: 497 TELLFTLVCCRPLIIPRTQPRDRSPSN 520

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BLAST of MC06g1136 vs. ExPASy Swiss-Prot
Match: Q9Z2E9 (Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 7.0e-05
Identity = 39/161 (24.22%), Postives = 76/161 (47.20%), Query Frame = 0


Query: 158 GHRFTASVILLMPESDFNRHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTF 217
           G  +  ++ L +PES  N+ +G+F +     +  G II++SS   ML +RS+ +++  T 
Sbjct: 103 GQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRIISTSSRSVMLHYRSQLLQVLDTL 162

Query: 218 LASIPLVLGISTESQELTFHMLKHKEVNHQRSKAIRVTIIPRVGTL-----ALPELYEAR 277
           L S  L+ G + + Q L   +      N         + +P  G +        ++Y A 
Sbjct: 163 LFSSLLLFGFAEQKQLLEVELYSDYREN---------SYVPTTGAIIEIHSKRIQMYGAY 222

Query: 278 IHINSQLPPMKQLLHRWRWTCYL--WTTFYTYLVFLVMFMY 312
           + I++    ++ LL+ +  TC      + +T+L  +V+F Y
Sbjct: 223 LRIHAHFTGLRYLLYNFPMTCAFVGVASNFTFLSVIVLFSY 254

[Close]

BLAST of MC06g1136 vs. ExPASy Swiss-Prot
Match: Q5E9P6 (Seipin OS=Bos taurus OX=9913 GN=BSCL2 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.7e-04
Identity = 38/161 (23.60%), Postives = 75/161 (46.58%), Query Frame = 0


Query: 158 GHRFTASVILLMPESDFNRHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTF 217
           G  +  ++ L +PES  N+ +G+F +     +  G II++SS   ML +RS  +++  T 
Sbjct: 103 GQPYRVTLELELPESPVNQDLGMFLVTISCYTRGGRIISTSSRSVMLHYRSSLLQMLDTL 162

Query: 218 LASIPLVLGISTESQELTFHMLKHKEVNHQRSKAIRVTIIPRVGTL-----ALPELYEAR 277
           + S  L+ G + + Q L   +      N         + +P  G +        ++Y A 
Sbjct: 163 VFSSLLLFGFAEQKQLLEVELYPEYREN---------SYVPTTGAIIEIHSKRIQMYGAY 222

Query: 278 IHINSQLPPMKQLLHRWRWTCYL--WTTFYTYLVFLVMFMY 312
           + I++    ++ LL+ +  TC      + +T+L  +V+F Y
Sbjct: 223 LRIHAHFTGLRYLLYNFPMTCAFVGVASNFTFLSVIVLFSY 254

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BLAST of MC06g1136 vs. NCBI nr
Match: XP_022149977.1 (seipin-1 [Momordica charantia])

HSP 1 Score: 702 bits (1813), Expect = 1.71e-253
Identity = 372/373 (99.73%), Postives = 372/373 (99.73%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA
Sbjct: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME
Sbjct: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV 180
           EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV
Sbjct: 121 EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV 180

Query: 181 FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK 240
           FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK
Sbjct: 181 FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK 240

Query: 241 HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY 300
           HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY
Sbjct: 241 HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY 300

Query: 301 TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM 360
           TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM
Sbjct: 301 TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM 360

Query: 361 RRKRKAAMFAYEE 373
           RRKRKAAMFAY E
Sbjct: 361 RRKRKAAMFAYRE 373

[Close]

BLAST of MC06g1136 vs. NCBI nr
Match: KAG7022197.1 (Seipin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 514 bits (1324), Expect = 7.34e-179
Identity = 278/408 (68.14%), Postives = 330/408 (80.88%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE ++SG  I  P+D FNK++FL A+LIY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 1   MDPDDELQQSGQLISKPSDLFNKIVFLQADLIYHAIAFLIAPISTLLSLISESFRRAEEA 60

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M++LL  AA  SA A+R W+E
Sbjct: 61  KSTVEYAVRKSPSAVAHRVRLAVRRLSYGLVAAAFMCMVMILLLGTAASVSAVAIRFWIE 120

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  R  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 121 EPVATRGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 180

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+S+PL+LG+STESQ+LT
Sbjct: 181 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSSLPLLLGLSTESQKLT 240

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LKHKE ++QRS AI+VTI PRVGT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 241 FPVLKHKEQSYQRSGAIQVTITPRVGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 300

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           W++FY YL FL +F+YFW+P+VFRA +L     N R AS S+E E+SFD+MA++TVELLR
Sbjct: 301 WSSFYLYLTFLAIFVYFWRPIVFRAVTLARFDFN-REASISKEVEKSFDEMAEVTVELLR 360

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 361 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 406

[Close]

BLAST of MC06g1136 vs. NCBI nr
Match: KAG6588277.1 (Seipin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514 bits (1324), Expect = 4.56e-178
Identity = 278/408 (68.14%), Postives = 330/408 (80.88%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE ++SG  I  P+D FNK++FL A+LIY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 53  MDPDDELQQSGQLISKPSDLFNKIVFLQADLIYHAIAFLIAPISTLLSLISESFRRAEEA 112

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M++LL  AA  SA A+R W+E
Sbjct: 113 KSTVEYAVRKSPSAVAHRVRLAVRRLSYGLVAAAFMCMVMILLLGTAASVSAVAIRFWIE 172

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  R  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 173 EPVATRGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 232

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+S+PL+LG+STESQ+LT
Sbjct: 233 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSSLPLLLGLSTESQKLT 292

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LKHKE ++QRS AI+VTI PRVGT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 293 FPVLKHKEQSYQRSGAIQVTITPRVGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 352

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           W++FY YL FL +F+YFW+P+VFRA +L     N R AS S+E E+SFD+MA++TVELLR
Sbjct: 353 WSSFYLYLTFLAIFVYFWRPIVFRAVTLARFDFN-REASISKEVEKSFDEMAEVTVELLR 412

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 413 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 458

[Close]

BLAST of MC06g1136 vs. NCBI nr
Match: XP_022933775.1 (seipin-1 [Cucurbita moschata])

HSP 1 Score: 512 bits (1319), Expect = 2.62e-177
Identity = 278/408 (68.14%), Postives = 331/408 (81.13%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE ++SG  I  P+D FNK++FL A+LIY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 53  MDPDDELQQSGRLISKPSDLFNKIVFLQADLIYHAIAFLIAPISTLLSLISESFRRAEEA 112

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M++LLA AA  SA A+R W+E
Sbjct: 113 KSTVEYAVRKSPSAVAHRVRLAVRRLSYGLVAAAFMCMVMILLLATAASVSAVAIRFWIE 172

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  R  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 173 EPVARRGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 232

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+S+PL+LG+STESQ+LT
Sbjct: 233 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSSLPLLLGLSTESQKLT 292

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LK+KE ++QRS AI+VTI PRVGT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 293 FPVLKYKEQSYQRSGAIQVTITPRVGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 352

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           W++FY YL FL +F+YFW+P+VFRA +L     N R AS S+E E+SFD+MA++TVELLR
Sbjct: 353 WSSFYLYLTFLAIFVYFWRPIVFRAVTLARFDFN-REASISKEVEKSFDEMAEVTVELLR 412

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 413 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 458

[Close]

BLAST of MC06g1136 vs. NCBI nr
Match: XP_023005649.1 (seipin-1 [Cucurbita maxima])

HSP 1 Score: 510 bits (1314), Expect = 2.52e-176
Identity = 275/408 (67.40%), Postives = 330/408 (80.88%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE +ESG  I  P+D FNK++F+ A++IY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 68  MDPDDELQESGRLIAKPSDLFNKIVFIQADIIYHAIAFLIAPISTLLSLISESFHRAEEA 127

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M +LLA AA  SA A+R W+E
Sbjct: 128 KSTVEYAVRKSPSAVAYRVRLAVRRLSYGLVAAAFMCMVMTLLLATAASVSAVAIRFWIE 187

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  +  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 188 EPVATKGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 247

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+ +PL+LG+STESQ+LT
Sbjct: 248 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSGLPLLLGLSTESQKLT 307

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LKHKE ++QRS AI+VTI PR+GT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 308 FPVLKHKEQSYQRSGAIQVTITPRIGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 367

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           WT+FY YL FL +F+YFW+P+VFRA ++    G +R AS S+E E+SFD+MA++TVELLR
Sbjct: 368 WTSFYFYLTFLAIFVYFWRPIVFRAVTVARF-GFDREASMSKEVEKSFDEMAEVTVELLR 427

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 428 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 473

[Close]

BLAST of MC06g1136 vs. ExPASy TrEMBL
Match: A0A6J1D9G7 (seipin-1 OS=Momordica charantia OX=3673 GN=LOC111018260 PE=4 SV=1)

HSP 1 Score: 702 bits (1813), Expect = 8.27e-254
Identity = 372/373 (99.73%), Postives = 372/373 (99.73%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA
Sbjct: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME
Sbjct: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV 180
           EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV
Sbjct: 121 EPVMVRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGV 180

Query: 181 FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK 240
           FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK
Sbjct: 181 FQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLK 240

Query: 241 HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY 300
           HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY
Sbjct: 241 HKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFY 300

Query: 301 TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM 360
           TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM
Sbjct: 301 TYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEM 360

Query: 361 RRKRKAAMFAYEE 373
           RRKRKAAMFAY E
Sbjct: 361 RRKRKAAMFAYRE 373

[Close]

BLAST of MC06g1136 vs. ExPASy TrEMBL
Match: A0A6J1F5S3 (seipin-1 OS=Cucurbita moschata OX=3662 GN=LOC111441088 PE=4 SV=1)

HSP 1 Score: 512 bits (1319), Expect = 1.27e-177
Identity = 278/408 (68.14%), Postives = 331/408 (81.13%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE ++SG  I  P+D FNK++FL A+LIY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 53  MDPDDELQQSGRLISKPSDLFNKIVFLQADLIYHAIAFLIAPISTLLSLISESFRRAEEA 112

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M++LLA AA  SA A+R W+E
Sbjct: 113 KSTVEYAVRKSPSAVAHRVRLAVRRLSYGLVAAAFMCMVMILLLATAASVSAVAIRFWIE 172

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  R  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 173 EPVARRGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 232

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+S+PL+LG+STESQ+LT
Sbjct: 233 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSSLPLLLGLSTESQKLT 292

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LK+KE ++QRS AI+VTI PRVGT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 293 FPVLKYKEQSYQRSGAIQVTITPRVGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 352

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           W++FY YL FL +F+YFW+P+VFRA +L     N R AS S+E E+SFD+MA++TVELLR
Sbjct: 353 WSSFYLYLTFLAIFVYFWRPIVFRAVTLARFDFN-REASISKEVEKSFDEMAEVTVELLR 412

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 413 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 458

[Close]

BLAST of MC06g1136 vs. ExPASy TrEMBL
Match: A0A6J1KZV1 (seipin-1 OS=Cucurbita maxima OX=3661 GN=LOC111498585 PE=4 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 1.22e-176
Identity = 275/408 (67.40%), Postives = 330/408 (80.88%), Query Frame = 0


Query: 1   MDSDDEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEA 60
           MD DDE +ESG  I  P+D FNK++F+ A++IY AIAF++APISTLLSL+SESF  AEEA
Sbjct: 68  MDPDDELQESGRLIAKPSDLFNKIVFIQADIIYHAIAFLIAPISTLLSLISESFHRAEEA 127

Query: 61  KQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWME 120
           K +VE AVR++PS  A RVRLA RR+SYGL AAA +C+ M +LLA AA  SA A+R W+E
Sbjct: 128 KSTVEYAVRKSPSAVAYRVRLAVRRLSYGLVAAAFMCMVMTLLLATAASVSAVAIRFWIE 187

Query: 121 EPVMVRERLNFDYTQAQPRAVFGAENNG-----KKNNLGIPVGHRFTASVILLMPESDFN 180
           EPV  +  LNFDYT A+PRA++G +  G     K  NLGIPVGH F+ SVILLMPES FN
Sbjct: 188 EPVATKGNLNFDYTVARPRALYGVDYRGGMKTKKTKNLGIPVGHTFSVSVILLMPESQFN 247

Query: 181 RHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELT 240
           R VGVFQL AELISTNGNII  SS PCMLRFRS PVR TRTFL+ +PL+LG+STESQ+LT
Sbjct: 248 RAVGVFQLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSGLPLLLGLSTESQKLT 307

Query: 241 FHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYL 300
           F +LKHKE ++QRS AI+VTI PR+GT ALPELYEA I INS+LP  K+L+HRWRWTC+L
Sbjct: 308 FPVLKHKEQSYQRSGAIQVTITPRIGTSALPELYEAHILINSKLPWTKELVHRWRWTCFL 367

Query: 301 WTTFYTYLVFLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLR 360
           WT+FY YL FL +F+YFW+P+VFRA ++    G +R AS S+E E+SFD+MA++TVELLR
Sbjct: 368 WTSFYFYLTFLAIFVYFWRPIVFRAVTVARF-GFDREASMSKEVEKSFDEMAEVTVELLR 427

Query: 361 KWQEMRRKRKAAMFAYEEEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           KWQEMRRKRKAA+F Y+E DVGSTSASSISCSR+Y  S FEEDVGDSE
Sbjct: 428 KWQEMRRKRKAALFGYDE-DVGSTSASSISCSREYIGSIFEEDVGDSE 473

[Close]

BLAST of MC06g1136 vs. ExPASy TrEMBL
Match: A0A1S3B8L6 (seipin-1 OS=Cucumis melo OX=3656 GN=LOC103487228 PE=4 SV=1)

HSP 1 Score: 482 bits (1240), Expect = 3.10e-166
Identity = 269/417 (64.51%), Postives = 320/417 (76.74%), Query Frame = 0


Query: 1   MDSDDEHEESG----LPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLH 60
           MDSD++ +E      + I  PTDFFNKL+FL A+LIY A+ FV+AP STLLSL +ESF  
Sbjct: 1   MDSDNQLQEPNRQIKIQIQKPTDFFNKLVFLQADLIYNAVGFVIAPASTLLSLFAESFQR 60

Query: 61  AEEAKQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMR 120
           AEE K +VESAVR++PS  A+RV++A RRVSYG  AA ++C+ MV+LL  A G S   +R
Sbjct: 61  AEETKHTVESAVRKSPSLVAQRVKVAVRRVSYGAVAAGMMCMVMVLLLIVAVGVSGLGIR 120

Query: 121 AWMEEPVMVRERLNFDYTQAQPRAVFGAENNG----KKNNLGIPVGHRFTASVILLMPES 180
            W+EEPV ++E+L FDYT+A+PRA+FG  N      KK NLGIPVGH F   V+LLMPES
Sbjct: 121 YWIEEPVDIKEKLRFDYTEARPRALFGMGNGNTMKMKKKNLGIPVGHTFFVCVVLLMPES 180

Query: 181 DFNRHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQ 240
            FNR  GVFQL AELISTNGNIITSSS PCMLRFRS PVR  RT L S P+++GISTE+Q
Sbjct: 181 QFNREFGVFQLSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQ 240

Query: 241 ELTFHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWT 300
            L+F +LKHKE N +RS AI+ TI PR+GT ALPELYEA I INS+LP MK+LL RWRWT
Sbjct: 241 RLSFPILKHKEENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWT 300

Query: 301 CYLWTTFYTYLVFLVMFMYFWKPVVFRAASLK-ELSGNNRGASASRE---AEESFDDMAD 360
           C+LWT+ Y YL+FL +FM FWKPV+FRA +L  ELS  ++     RE   A+ES D+MA+
Sbjct: 301 CFLWTSIYLYLMFLAIFMLFWKPVMFRAMTLTPELSDFDQDPRR-RELEGADESLDEMAE 360

Query: 361 ITVELLRKWQEMRRKRKAAMFAYE--EEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           ITVELLRKWQEMRRKRKAAMF Y   EEDVGSTSASSISCSRDY  + FEEDVGDSE
Sbjct: 361 ITVELLRKWQEMRRKRKAAMFGYGSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSE 416

[Close]

BLAST of MC06g1136 vs. ExPASy TrEMBL
Match: A0A0A0M367 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G657470 PE=4 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 1.45e-163
Identity = 263/417 (63.07%), Postives = 319/417 (76.50%), Query Frame = 0


Query: 1   MDSDDEHEESG--LPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAE 60
           MDSD E EE    + I  PTDFFNKL+FL A+LIY A+ FV+AP  TLLSL +ESF  AE
Sbjct: 1   MDSDYELEEPNRQIQIRKPTDFFNKLVFLQADLIYNAMEFVIAPAYTLLSLFAESFQRAE 60

Query: 61  EAKQSVESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAW 120
           E K +VESAVR++PS  A+RV++AARR+SYG  AA ++C+ MV+LL  A G S   +R W
Sbjct: 61  ETKHTVESAVRKSPSVVAQRVKVAARRMSYGAVAAGMMCMVMVLLLVVAMGVSGLGIRYW 120

Query: 121 MEEPVMVRERLNFDYTQAQPRAVFGAENNG----KKNNLGIPVGHRFTASVILLMPESDF 180
           +EEPV V+E+L FDYT+A+PRA+FG  N      KK NLGIPVGH F   V+LLMPES F
Sbjct: 121 IEEPVDVKEKLKFDYTEARPRALFGTGNGNTMKMKKKNLGIPVGHTFYVCVVLLMPESQF 180

Query: 181 NRHVGVFQLRAELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQEL 240
           NR  GVFQL AELISTNGN+ITSSS PCMLRFRS PVRL RT + S P+++GIS+E+Q L
Sbjct: 181 NREFGVFQLSAELISTNGNVITSSSQPCMLRFRSAPVRLARTIITSFPVLVGISSEAQRL 240

Query: 241 TFHMLKHKEVNHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCY 300
           +F +L+HKE   +RS AI+VTI PR+GT ALPELYEA I INS+ P MK+LL RW+WTC+
Sbjct: 241 SFPILEHKEEKQERSAAIQVTISPRIGTSALPELYEADILINSKPPKMKELLRRWQWTCF 300

Query: 301 LWTTFYTYLVFLVMFMYFWKPVVFRAASLK------ELSGNNRGASASREAEESFDDMAD 360
           +WT+ Y YL+F+VMFM+FWKPV+FRA +L+      +L  + R      + E S D+MA+
Sbjct: 301 VWTSMYLYLMFVVMFMFFWKPVMFRAMTLRPHQLIRDLDQDPRRREVEGD-ESSLDEMAE 360

Query: 361 ITVELLRKWQEMRRKRKAAMFAYE--EEDVGSTSASSISCSRDYTVSGFEEDVGDSE 403
           ITVELLRKWQEMRRKRKAAMF Y   EEDVGSTSASSISCSRDYT + FEEDVGDSE
Sbjct: 361 ITVELLRKWQEMRRKRKAAMFGYGSGEEDVGSTSASSISCSRDYTAAVFEEDVGDSE 416

[Close]

BLAST of MC06g1136 vs. TAIR 10
Match: AT5G16460.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 227.3 bits (578), Expect = 2.3e-59
Identity = 138/363 (38.02%), Postives = 209/363 (57.58%), Query Frame = 0


Query: 5   DEHEESGLPIPAPTDFFNKLIFLAAELIYAAIAFVLAPISTLLSLLSESFLHAEEAKQSV 64
           ++  +  +P P   D+F  L+ + A+LIY A+  + +P      LL  S+  A     + 
Sbjct: 11  EQDNQLKIPEPLRADWFMVLVTIQADLIYNALVVLSSP----FFLLYRSYRRAVVTVSAA 70

Query: 65  ESAVRRAPSDAARRVRLAARRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVM 124
           E AV+RAP+  A       RR  +G+  A  V + MV+ L  A       +  ++E+PV+
Sbjct: 71  EKAVKRAPAQIAGGAGRVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVV 130

Query: 125 VRERLNFDYTQAQPRAVFGAENNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGVFQLR 184
           VR+RL FDYT+  P AVF  +   KK +  +PVGH    S++L MPES+ NR +GVFQL+
Sbjct: 131 VRDRLFFDYTEENPSAVFSFDK--KKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQLK 190

Query: 185 AELISTNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLKHKEV 244
            EL+S  G  I  SS PCMLRFRS+P+RL RTF+ S+PL+ GI+ E+Q +    LKH+E 
Sbjct: 191 VELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKHQE- 250

Query: 245 NHQRSKAIRVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYLV 304
              R+KA+R T+IPR  T  LP+LYEA I INS+ P +K++ + W+WT  +WT+ Y Y+ 
Sbjct: 251 KMPRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYLYVA 310

Query: 305 FLVMFMYFWKPVVFRAASLKELSGNNRGASASREAEESFDDMADITVELLRKWQEMRRKR 364
            L   ++ ++PV+F   S + +S      S + E E   ++   +     R+ +   R+R
Sbjct: 311 ILTALLWCFRPVLFPYTSSRTIS-----ESENLEIEVVEEEQEQVMERRRRERRNQPRRR 361

Query: 365 KAA 368
             A
Sbjct: 371 NFA 361

[Close]

BLAST of MC06g1136 vs. TAIR 10
Match: AT2G34380.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-27
Identity = 77/252 (30.56%), Postives = 137/252 (54.37%), Query Frame = 0


Query: 84  RRVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVMVRERLNFDYTQAQPRA--- 143
           RRV++GL  A  V + +  LL +A   S   +     EP++++E LNFDYT++ P A   
Sbjct: 232 RRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSSPEAYVP 291

Query: 144 -------VFGAE-----NNGKKNNLGIPVGHRFTASVILLMPESDFNRHVGVFQLRAELI 203
                   FG         GK   L      R   +V + +PES++NR++G+FQ+R + +
Sbjct: 292 ISSCAGVAFGLSGKESIETGKVKGL----KDRTEITVSMTLPESEYNRNLGMFQVRVDFL 351

Query: 204 STNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHMLKHKEVNHQR 263
           S +G+++ SS  PCM++F S P+RL +T L   PLV G  +E Q L   +    E +   
Sbjct: 352 SASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGLVEKDIIP 411

Query: 264 SKAIRVTIIPRV---GTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYLVF 318
           +  +++ I  R        +PE+Y+A + + S+LP +K+++  WR T ++W +   +++ 
Sbjct: 412 TACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWISMSLFIME 471

[Close]

BLAST of MC06g1136 vs. TAIR 10
Match: AT1G29760.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 106.3 bits (264), Expect = 5.8e-23
Identity = 76/267 (28.46%), Postives = 133/267 (49.81%), Query Frame = 0


Query: 85  RVSYGLAAAALVCVAMVVLLAAAAGASAAAMRAWMEEPVMVRERLNFDYTQAQPRAVF-- 144
           +  +G+  A  V + +  LL ++       +    ++P  V+E LNFDYT+  P A    
Sbjct: 257 KFGWGMFWAVYVGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPI 316

Query: 145 ----GAENNG---KKNNLG-------IPVGHRFTASVILLMPESDFNRHVGVFQLRAELI 204
               G E  G   + N +        IP   +    + + +PES +N+++G+FQ+R + +
Sbjct: 317 SSCAGVECEGSCKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQVRVDFL 376

Query: 205 STNGNIITSSSLPCMLRFRSRPVRLTRTFLASIPLVLGISTESQELTFHM--LKHKEVNH 264
           S +G  I S   PCMLRFRS P+RL +TF   +PLV G  +E Q L+  +     K++  
Sbjct: 377 SVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPT 436

Query: 265 QRSKAI---RVTIIPRVGTLALPELYEARIHINSQLPPMKQLLHRWRWTCYLWTTFYTYL 324
              K I   R    P  G   +PELY+A + + S LP  ++++ +WR T ++W +   ++
Sbjct: 437 ACLKIIIEQRAEFRPGAG---IPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFI 496

Query: 325 VFLVMFMYFWKPVVFRAASLKELSGNN 331
             L+  +   +P++      ++ S +N
Sbjct: 497 TELLFTLVCCRPLIIPRTQPRDRSPSN 520

The following BLAST results are available for this feature:

BLAST of MC06g1136 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFD9	3.2e-58	38.02	Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1	[more]
Q8L615	1.4e-26	30.56	Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1	[more]
F4I340	8.2e-22	28.46	Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1	[more]
Q9Z2E9	7.0e-05	24.22	Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2	[more]
Q5E9P6	2.7e-04	23.60	Seipin OS=Bos taurus OX=9913 GN=BSCL2 PE=2 SV=1	[more]

BLAST of MC06g1136 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149977.1	1.71e-253	99.73	seipin-1 [Momordica charantia]	[more]
KAG7022197.1	7.34e-179	68.14	Seipin-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6588277.1	4.56e-178	68.14	Seipin-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022933775.1	2.62e-177	68.14	seipin-1 [Cucurbita moschata]	[more]
XP_023005649.1	2.52e-176	67.40	seipin-1 [Cucurbita maxima]	[more]

BLAST of MC06g1136 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9G7	8.27e-254	99.73	seipin-1 OS=Momordica charantia OX=3673 GN=LOC111018260 PE=4 SV=1	[more]
A0A6J1F5S3	1.27e-177	68.14	seipin-1 OS=Cucurbita moschata OX=3662 GN=LOC111441088 PE=4 SV=1	[more]
A0A6J1KZV1	1.22e-176	67.40	seipin-1 OS=Cucurbita maxima OX=3661 GN=LOC111498585 PE=4 SV=1	[more]
A0A1S3B8L6	3.10e-166	64.51	seipin-1 OS=Cucumis melo OX=3656 GN=LOC103487228 PE=4 SV=1	[more]
A0A0A0M367	1.45e-163	63.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G657470 PE=4 SV=1	[more]

BLAST of MC06g1136 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16460.1	2.3e-59	38.02	Putative adipose-regulatory protein (Seipin)	[more]
AT2G34380.1	1.0e-27	30.56	Putative adipose-regulatory protein (Seipin)	[more]
AT1G29760.1	5.8e-23	28.46	Putative adipose-regulatory protein (Seipin)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009617	Seipin family	PFAM	PF06775	Seipin	coord: 102..300 e-value: 1.0E-35 score: 123.6
IPR009617	Seipin family	PANTHER	PTHR21212	BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN	coord: 10..403
None	No IPR available	PANTHER	PTHR21212:SF5	SEIPIN-1	coord: 10..403

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1136.1	MC06g1136.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0140042	lipid droplet formation
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0048316	seed development
biological_process	GO:0019915	lipid storage
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane