MC06g1081 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1081
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDUF4220 domain-containing protein
LocationMC06: 10993564 .. 10995393 (+)
RNA-Seq ExpressionMC06g1081
SyntenyMC06g1081
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCTATCTTCAGTTTTACTCTGCAAGTTATTCTCATTCCTATGGGGTTGCGTAGAAAGTACAGCTCCAACAATGCCCTCCGCTTGCTTCTGTTGCTTTTTTATTTGTCCGCAGACTGGGCCGCCACCACTTCGCTCGGCACCCTCGTTAAGTTCTATGGCAGCTACGAAGATGCCTTCTTCGGACGACTCTTCATCTTGGCGCCATTTATGCTGCTCCACCTCGGTGGCTCGGACACCATTACCGCCTACTCCATGGAAGACAACGATCTCTGGTACAGGTCCTTTTTTGGTTTCTTTGTTCAAGTTGGGATCGCGTTTTACATCCTTCTACTAGCTTTGCAACCCCAACATCTGGATTTTTTGGGGATTCCTATATTTGTGGCTGGGATCATCAAATATGGCGAGAGGATTTGGGTTTTCAGATCTACTTCTACCCAGCGCCTGCCAGACCTATTGCTTTCAACTACTCGTTTCAGTCCTATACAAATCAACGCTGCAAAGTCTAAGCATTTGCATCTTGAATTTCCCATACAAATCAACGTCGATCATGAAGCCACACAAAACCCTCATTTCAGTCACCTTCACTTGCTGCATATTGCTTATTATTTCTTCAAAACAAACAAATTCCTGTTTGTAGATTTGACTCTGACCTCCTATGATCTTCAACAAAGCCTTCACTACTTTATGCAATTTGACTCTAGAGAAGCTTTCAAAGTCATTGAGCTTGAGCTTGGATTTATGTATGATTTCTTCTACACAAAAGCCTCAATCATCCATTCTCGTTGGGGTCCTATTCTTCGTCTTACTACCTTATTTTCCATTGTTGTTGTCATTGTCACCTTTCATATAGACCACTTCGATCCTGGATTTAATAATCCATTGACCAATATTTTGACATTAATCCTGCTCTATGGAGCTTTGAGTCTTGAGATCTCTTCGTTTATTTTGTTTCTATGCTCAGACTGGAATGTAATCCGGTTGACCAAGAGCTCTTACTCTCTCGCTCATTTGACATTTAAAGCTATCTCTCGTTGCGGATGGTCTGTCAAAAAATATAGGTGGTCAAATTCTGTTCGACAGTACAATTTAATATCTTGTTGCTTGAAAGAGACCAAATATGGGAGATACTGCAAGTACTTTAGAACTTCTTACATCTCCAAAATCATGACAGCTTCACGAAACATCTCTGATGAACTCAAAACACGGATCTTTCAACAACTAACGCAAAAGTTGGAAGTTAATGAAGAGAACAGAAAGCTACCTGGTTGGATTCTAAGAAAGCACAACTGTTATAACCAACTTGGCTGGAGTCTGGAATTAGATTCCGACCAGAGCATCCTCCTCTGGCACATTGCTACCAATATCTGCTATCATCGTGATAAAGAAACTGAGGCATCAAATTGTTCTCTTCTTGAGGATGGCACATTGCTGTCTGATTTTTTGACCTACCTTTTAGTGTATCATCATTCGTTATTTCTCGATGGGATGAGTGAAATAAGATTTTGTGAAACTGTTGACAGTGCAATTGAATTTATGCAGCAGAGAAAATCCATAGAAACAACTAGTGATGCTTGTAAGAGTATGTTGGATTTGGAGACGAGTACGGTGTACAAAGATGCTGGCAACTCAGTCTTCTTTGGTGGGTGTCGGTTGGCGCGAGAGCTTCAAGGGCTAGAAGGGTGTGAGAGATGGGAAATAATCAACCATGTGTGGGTAGAGATGCTTGCTAATATTTCATGTGAATGCAGATGGTATGAGCATGCTAAGAAGCTCAGACATGGCGGTAATTTGCTCACTCATGTTTGGCTTTTGATGCACCATCTTGGA

mRNA sequence

CTTCCTATCTTCAGTTTTACTCTGCAAGTTATTCTCATTCCTATGGGGTTGCGTAGAAAGTACAGCTCCAACAATGCCCTCCGCTTGCTTCTGTTGCTTTTTTATTTGTCCGCAGACTGGGCCGCCACCACTTCGCTCGGCACCCTCGTTAAGTTCTATGGCAGCTACGAAGATGCCTTCTTCGGACGACTCTTCATCTTGGCGCCATTTATGCTGCTCCACCTCGGTGGCTCGGACACCATTACCGCCTACTCCATGGAAGACAACGATCTCTGGTACAGGTCCTTTTTTGGTTTCTTTGTTCAAGTTGGGATCGCGTTTTACATCCTTCTACTAGCTTTGCAACCCCAACATCTGGATTTTTTGGGGATTCCTATATTTGTGGCTGGGATCATCAAATATGGCGAGAGGATTTGGGTTTTCAGATCTACTTCTACCCAGCGCCTGCCAGACCTATTGCTTTCAACTACTCGTTTCAGTCCTATACAAATCAACGCTGCAAAGTCTAAGCATTTGCATCTTGAATTTCCCATACAAATCAACGTCGATCATGAAGCCACACAAAACCCTCATTTCAGTCACCTTCACTTGCTGCATATTGCTTATTATTTCTTCAAAACAAACAAATTCCTGTTTGTAGATTTGACTCTGACCTCCTATGATCTTCAACAAAGCCTTCACTACTTTATGCAATTTGACTCTAGAGAAGCTTTCAAAGTCATTGAGCTTGAGCTTGGATTTATGTATGATTTCTTCTACACAAAAGCCTCAATCATCCATTCTCGTTGGGGTCCTATTCTTCGTCTTACTACCTTATTTTCCATTGTTGTTGTCATTGTCACCTTTCATATAGACCACTTCGATCCTGGATTTAATAATCCATTGACCAATATTTTGACATTAATCCTGCTCTATGGAGCTTTGAGTCTTGAGATCTCTTCGTTTATTTTGTTTCTATGCTCAGACTGGAATGTAATCCGGTTGACCAAGAGCTCTTACTCTCTCGCTCATTTGACATTTAAAGCTATCTCTCGTTGCGGATGGTCTGTCAAAAAATATAGGTGGTCAAATTCTGTTCGACAGTACAATTTAATATCTTGTTGCTTGAAAGAGACCAAATATGGGAGATACTGCAAGTACTTTAGAACTTCTTACATCTCCAAAATCATGACAGCTTCACGAAACATCTCTGATGAACTCAAAACACGGATCTTTCAACAACTAACGCAAAAGTTGGAAGTTAATGAAGAGAACAGAAAGCTACCTGGTTGGATTCTAAGAAAGCACAACTGTTATAACCAACTTGGCTGGAGTCTGGAATTAGATTCCGACCAGAGCATCCTCCTCTGGCACATTGCTACCAATATCTGCTATCATCGTGATAAAGAAACTGAGGCATCAAATTGTTCTCTTCTTGAGGATGGCACATTGCTGTCTGATTTTTTGACCTACCTTTTAGTGTATCATCATTCGTTATTTCTCGATGGGATGAGTGAAATAAGATTTTGTGAAACTGTTGACAGTGCAATTGAATTTATGCAGCAGAGAAAATCCATAGAAACAACTAGTGATGCTTGTAAGAGTATGTTGGATTTGGAGACGAGTACGGTGTACAAAGATGCTGGCAACTCAGTCTTCTTTGGTGGGTGTCGGTTGGCGCGAGAGCTTCAAGGGCTAGAAGGGTGTGAGAGATGGGAAATAATCAACCATGTGTGGGTAGAGATGCTTGCTAATATTTCATGTGAATGCAGATGGTATGAGCATGCTAAGAAGCTCAGACATGGCGGTAATTTGCTCACTCATGTTTGGCTTTTGATGCACCATCTTGGA

Coding sequence (CDS)

CTTCCTATCTTCAGTTTTACTCTGCAAGTTATTCTCATTCCTATGGGGTTGCGTAGAAAGTACAGCTCCAACAATGCCCTCCGCTTGCTTCTGTTGCTTTTTTATTTGTCCGCAGACTGGGCCGCCACCACTTCGCTCGGCACCCTCGTTAAGTTCTATGGCAGCTACGAAGATGCCTTCTTCGGACGACTCTTCATCTTGGCGCCATTTATGCTGCTCCACCTCGGTGGCTCGGACACCATTACCGCCTACTCCATGGAAGACAACGATCTCTGGTACAGGTCCTTTTTTGGTTTCTTTGTTCAAGTTGGGATCGCGTTTTACATCCTTCTACTAGCTTTGCAACCCCAACATCTGGATTTTTTGGGGATTCCTATATTTGTGGCTGGGATCATCAAATATGGCGAGAGGATTTGGGTTTTCAGATCTACTTCTACCCAGCGCCTGCCAGACCTATTGCTTTCAACTACTCGTTTCAGTCCTATACAAATCAACGCTGCAAAGTCTAAGCATTTGCATCTTGAATTTCCCATACAAATCAACGTCGATCATGAAGCCACACAAAACCCTCATTTCAGTCACCTTCACTTGCTGCATATTGCTTATTATTTCTTCAAAACAAACAAATTCCTGTTTGTAGATTTGACTCTGACCTCCTATGATCTTCAACAAAGCCTTCACTACTTTATGCAATTTGACTCTAGAGAAGCTTTCAAAGTCATTGAGCTTGAGCTTGGATTTATGTATGATTTCTTCTACACAAAAGCCTCAATCATCCATTCTCGTTGGGGTCCTATTCTTCGTCTTACTACCTTATTTTCCATTGTTGTTGTCATTGTCACCTTTCATATAGACCACTTCGATCCTGGATTTAATAATCCATTGACCAATATTTTGACATTAATCCTGCTCTATGGAGCTTTGAGTCTTGAGATCTCTTCGTTTATTTTGTTTCTATGCTCAGACTGGAATGTAATCCGGTTGACCAAGAGCTCTTACTCTCTCGCTCATTTGACATTTAAAGCTATCTCTCGTTGCGGATGGTCTGTCAAAAAATATAGGTGGTCAAATTCTGTTCGACAGTACAATTTAATATCTTGTTGCTTGAAAGAGACCAAATATGGGAGATACTGCAAGTACTTTAGAACTTCTTACATCTCCAAAATCATGACAGCTTCACGAAACATCTCTGATGAACTCAAAACACGGATCTTTCAACAACTAACGCAAAAGTTGGAAGTTAATGAAGAGAACAGAAAGCTACCTGGTTGGATTCTAAGAAAGCACAACTGTTATAACCAACTTGGCTGGAGTCTGGAATTAGATTCCGACCAGAGCATCCTCCTCTGGCACATTGCTACCAATATCTGCTATCATCGTGATAAAGAAACTGAGGCATCAAATTGTTCTCTTCTTGAGGATGGCACATTGCTGTCTGATTTTTTGACCTACCTTTTAGTGTATCATCATTCGTTATTTCTCGATGGGATGAGTGAAATAAGATTTTGTGAAACTGTTGACAGTGCAATTGAATTTATGCAGCAGAGAAAATCCATAGAAACAACTAGTGATGCTTGTAAGAGTATGTTGGATTTGGAGACGAGTACGGTGTACAAAGATGCTGGCAACTCAGTCTTCTTTGGTGGGTGTCGGTTGGCGCGAGAGCTTCAAGGGCTAGAAGGGTGTGAGAGATGGGAAATAATCAACCATGTGTGGGTAGAGATGCTTGCTAATATTTCATGTGAATGCAGATGGTATGAGCATGCTAAGAAGCTCAGACATGGCGGTAATTTGCTCACTCATGTTTGGCTTTTGATGCACCATCTTGGA

Protein sequence

LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAFFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVRQYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLG
Homology
BLAST of MC06g1081 vs. NCBI nr
Match: XP_022141971.1 (uncharacterized protein LOC111012216 [Momordica charantia])

HSP 1 Score: 1235 bits (3196), Expect = 0.0
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 60
           LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF
Sbjct: 23  LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 82

Query: 61  FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD 120
           FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD
Sbjct: 83  FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD 142

Query: 121 FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI 180
           FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI
Sbjct: 143 FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI 202

Query: 181 NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV 240
           NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV
Sbjct: 203 NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV 262

Query: 241 IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT 300
           IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT
Sbjct: 263 IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT 322

Query: 301 LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR 360
           LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR
Sbjct: 323 LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR 382

Query: 361 QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK 420
           QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK
Sbjct: 383 QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK 442

Query: 421 LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD 480
           LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD
Sbjct: 443 LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD 502

Query: 481 FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD 540
           FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD
Sbjct: 503 FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD 562

Query: 541 AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT 600
           AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT
Sbjct: 563 AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT 622

Query: 601 HVWLLMHHLG 610
           HVWLLMHHLG
Sbjct: 623 HVWLLMHHLG 632

BLAST of MC06g1081 vs. NCBI nr
Match: KAA0037446.1 (uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK01920.1 uncharacterized protein E5676_scaffold808G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 473 bits (1217), Expect = 5.59e-156
Identity = 291/622 (46.78%), Postives = 383/622 (61.58%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS--YEDA--- 64
           S  +Q++L+PMGL+RK+SS+  LR  LL+ Y  +DW A  S   LV+ YG+  Y+D    
Sbjct: 24  SLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  -FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 124
            +  R F LA F+LLHLGGSDTITAYSMEDN+LW R+       +  + YI L AL P  
Sbjct: 84  NYIIRAF-LAHFLLLHLGGSDTITAYSMEDNELWLRTLLSLLAVLAASIYIFLQALLPTS 143

Query: 125 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPI 184
           L+++ IP+ +AGIIK  E+IW  RS S +RL D L  +T  SPI          H E  +
Sbjct: 144 LNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEV 203

Query: 185 QINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREA 244
           Q   D E           +LHIAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F S  A
Sbjct: 204 Q---DFE-----------MLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLSYYEKFQSNSA 263

Query: 245 FKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTN 304
           FK+IELELGFMYDFFYTKASI HS  G + RLTT  S+ + I+T+ +      + +   N
Sbjct: 264 FKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLID-KQEYPSTYVN 323

Query: 305 ILTLILLYGALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
           ++ L L +GALS+EI S  L L SDWNVI L  T+S S  L  L  K IS CGWS KK R
Sbjct: 324 LIFL-LFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRY-CKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLE 424
            SNS+ QYNLIS CLK+     Y CK+  T  ++   +  R IS+ L+  IFQQL +KL 
Sbjct: 384 CSNSISQYNLISHCLKQKNDDSYYCKFHNTKTMAAF-SVQRPISNNLEAHIFQQLKKKLV 443

Query: 425 VNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYH-----RDKETEASN 484
           +N+E            + YN++GWSL+LD DQSILLWHIAT+ CY+     ++ E  + +
Sbjct: 444 LNQEY----------DSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSES 503

Query: 485 CSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKS 544
           C   +D   LS+FL Y +V+H SLF  GMS+IR   T +  +E +Q +K     S+  K+
Sbjct: 504 CIPPQDSISLSNFLAYFIVHHPSLFPSGMSQIRHKATSEDVLELLQDKKLGRCNSNMLKN 563

Query: 545 MLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEH 604
            L+L+   V ++   S+    CRLA  L+ LE  ++WEII +VWVE+L  ISCE  WY+H
Sbjct: 564 -LELKIEVVKEERKESMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDH 607

Query: 605 AKKLRHGGNLLTHVWLLMHHLG 610
           AK+L  GGNL+T VW+LMHHLG
Sbjct: 624 AKQLTQGGNLVTRVWILMHHLG 607

BLAST of MC06g1081 vs. NCBI nr
Match: XP_004139148.1 (uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical protein Csa_007023 [Cucumis sativus])

HSP 1 Score: 467 bits (1201), Expect = 1.29e-153
Identity = 290/620 (46.77%), Postives = 378/620 (60.97%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS-----YEDA 64
           S  +Q++L+P+GL+RK SS+  LR  LL+ Y  +DW A  S   LV+ YG+     + D 
Sbjct: 24  SLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHL 124
            +     LA F+LLHLGGSDTITAYSMEDN+LW R+       +  + YI L AL P  L
Sbjct: 84  MYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAILAASIYIFLQALLPTSL 143

Query: 125 DFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQ 184
           +++ IP+ +AG+IK  E+IW  RS S +RL D L  +T  SPI          H E  +Q
Sbjct: 144 NYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEVQ 203

Query: 185 INVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREAF 244
              D E           +L IAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F+S+ AF
Sbjct: 204 ---DFE-----------VLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 263

Query: 245 KVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNI 304
           K+IELELGFMYDFFYTKASI HS  G + RLTT  S+V+  +T+ +   D     P T +
Sbjct: 264 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCL--IDKQ-EYPSTYV 323

Query: 305 LTLILLY-GALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
             + LL+ GALS+EI S  LFL SDWNVI L  T+S S  L  L  K IS CGWS+KK R
Sbjct: 324 NLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEV 424
            SNS+ QYNLIS CL++     Y K+  T  I+   +  R IS+ L+  IFQQL QKL +
Sbjct: 384 CSNSISQYNLISHCLEQKNDSYYFKFPSTKTIAAF-SVQRPISNNLEAHIFQQLKQKLVL 443

Query: 425 NEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRD---KETEAS-NCS 484
           N+E              YN++GWSL+LD DQSIL+WHIAT+ CYH     KE+E S +C 
Sbjct: 444 NQEY----------DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCI 503

Query: 485 LLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSML 544
             +D   LS+FL Y +V+H SLF  GMS+IR   T +  +E +Q  K     S+  K+ L
Sbjct: 504 PPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKN-L 563

Query: 545 DLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAK 604
           +L    V ++   S      RLA  L+ LE  ++WEII +VWVE+L  ISCEC WY+HAK
Sbjct: 564 ELNIEVVKEERKESRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRISCECEWYDHAK 605

Query: 605 KLRHGGNLLTHVWLLMHHLG 610
           +L  GG+L+T VW+LMHHLG
Sbjct: 624 QLTQGGSLVTRVWILMHHLG 605

BLAST of MC06g1081 vs. NCBI nr
Match: XP_008458716.1 (PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo])

HSP 1 Score: 464 bits (1193), Expect = 2.25e-152
Identity = 288/622 (46.30%), Postives = 379/622 (60.93%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS--YEDA--- 64
           S  +Q++L+PMGL+RK+SS+   R  LL+ Y  +DW A  S   LV+ YG+  Y+D    
Sbjct: 24  SLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  -FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 124
            +  R F LA F+LLHLGG DTITAYSMEDN+LW R+       +  + YI L AL P  
Sbjct: 84  NYIIRAF-LAHFLLLHLGGFDTITAYSMEDNELWLRTLLSLLAVLAASIYIFLQALLPTS 143

Query: 125 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPI 184
           L+++ IP+ +AGIIK  E+IW  RS S +RL D L  +T  SPI          H E  +
Sbjct: 144 LNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEV 203

Query: 185 QINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREA 244
           Q   D E           +LHIAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F S  A
Sbjct: 204 Q---DFE-----------MLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLCYYEKFQSNSA 263

Query: 245 FKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTN 304
           FK+IELELGFMYDFFYTKASI HS  G + RLTT  S+ + I+T+ +      + +   N
Sbjct: 264 FKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLID-KQEYPSTYVN 323

Query: 305 ILTLILLYGALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
           ++ L L +GALS+EI S  L L SDWNVI L  T+S S  L  L  K IS CGWS KK R
Sbjct: 324 LIFL-LFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRY-CKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLE 424
            SNS+ QYNLIS CLK+     Y CK+  T  ++   +  R IS+ L+  IFQQL +KL 
Sbjct: 384 CSNSISQYNLISHCLKQKNDDSYYCKFHNTKTMAAF-SVQRPISNNLEAHIFQQLKKKLV 443

Query: 425 VNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYH-----RDKETEASN 484
           +N+E            + YN++GWSL+LD DQSILLWHIAT+ CY+     ++ E  + +
Sbjct: 444 LNQEY----------DSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSES 503

Query: 485 CSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKS 544
           C   +D   LS+FL Y +V+H SLF   MS+IR   T +  +E +Q +K     S+  K+
Sbjct: 504 CIPPQDSISLSNFLAYFIVHHPSLFPSRMSQIRHKATSEDVLELLQDKKLGRCNSNMLKN 563

Query: 545 MLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEH 604
            L+L+   V ++   S+    CRLA  L+ LE  ++WEII +VWVE+L  ISCE  WY+H
Sbjct: 564 -LELKIEVVKEERKESMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDH 607

Query: 605 AKKLRHGGNLLTHVWLLMHHLG 610
           AK L  GGNL+T VW+LMHHLG
Sbjct: 624 AKHLTQGGNLVTRVWILMHHLG 607

BLAST of MC06g1081 vs. NCBI nr
Match: XP_021279036.1 (uncharacterized protein LOC110412747 [Herrania umbratica])

HSP 1 Score: 433 bits (1113), Expect = 4.74e-140
Identity = 266/646 (41.18%), Postives = 377/646 (58.36%), Query Frame = 0

Query: 1   LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 60
           L + S +LQ+IL   G RRKY + N +  +L L YLSAD+ AT SLG L    G  +D  
Sbjct: 20  LVLLSLSLQIILSFFGNRRKYIARNWISFILWLAYLSADYVATVSLGILASNQGDLDDDS 79

Query: 61  FGRLFIL--APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 120
                +   APF+LLHLGG DTITAYS+EDN+LW R   G   QVG+AFY+ + +L+P  
Sbjct: 80  LDNAIMSFWAPFLLLHLGGPDTITAYSLEDNELWLRHLLGLVTQVGVAFYVFIRSLKPMK 139

Query: 121 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLE--F 180
           L+F+ IP+F+AG +KYGER WV RS S+Q   + LL      P      +   L     F
Sbjct: 140 LNFVAIPMFIAGTVKYGERTWVLRSASSQHFRESLLQRPDPGPNYAKFMEDYKLKEREGF 199

Query: 181 PIQINVDHEATQNPHFSHLH---------LLHIAYYFFKTNKFLFVDLTLTSYDLQQSLH 240
            +    +   T  P  +            +L  AY FF T K LF DL L+ +D+++S  
Sbjct: 200 ELSCTANQAFTVVPRTNSFQGGSLIPDGAILIAAYDFFNTCKRLFADLILSFHDIEKSHS 259

Query: 241 YFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFH-IDH 300
           YF +    EAFKVIE+ELGF+YD FYTKASI++S WG  LR  +L S ++ + TF  ID 
Sbjct: 260 YFQRCHWDEAFKVIEVELGFVYDLFYTKASIVYSNWGVFLRSVSLSSTIITLATFTAIDR 319

Query: 301 FDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRC 360
              GF     +I T +LL GA+ LEI + ++ L S+W ++RL+K        T KAIS  
Sbjct: 320 --QGFKTVDVSI-TFLLLIGAIFLEIYAILVLLSSEWTMLRLSKREKFPVSQTSKAISTF 379

Query: 361 GWSVKKYRWSNSVRQYNLISCCLKETKYGRYCKYFRTSYISKIM---TASRNISDELKTR 420
            +   K RWSN + QY+LI  C +    G      R  +I K +     S ++S  LK  
Sbjct: 380 KFITSKNRWSNYMEQYSLIGSCFRGEP-GDLHGVQRRGWIHKHVHGNITSESVSPCLKEF 439

Query: 421 IFQQLTQKLEVNEE---NRKLPGW----ILRKHNCYNQLGWSLELDSDQSILLWHIATNI 480
           +F++L +K +V      +R+L       +L + NC ++LGWS+E++ D SILLWHIAT++
Sbjct: 440 VFEELVEKSKVASNFSISRQLCACRGEQVLGEKNCLDRLGWSVEVEFDHSILLWHIATSL 499

Query: 481 CYHRDKETEASNC--SLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQ 540
           CY  D++   S+   S  +   L+S++L Y+LV   S+  +G+ +IRF +T+  AIEF+Q
Sbjct: 500 CYCYDQKRNPSSVLDSRCKVSKLISEYLLYILVKRPSMMPNGIGQIRFQDTICEAIEFIQ 559

Query: 541 QRKSIETTSDACKSMLDLET----STVYKDAGNSVFFGGCRLARELQGLEG------CER 600
           +RK I   S AC+ +L ++T    + V  D   SV F  CRLA+EL  +E        E+
Sbjct: 560 ERKFISNASLACEKLLQVDTRIEPAIVKGDRSKSVLFDACRLAKELHSVEQERKWHTNEK 619

Query: 601 WEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLG 610
           WE+++HVW+EML+  + +CRW +HA++LR GG LLTHVWLLM HLG
Sbjct: 620 WELVSHVWLEMLSYAAGQCRWNQHAQQLRRGGELLTHVWLLMAHLG 661

BLAST of MC06g1081 vs. ExPASy TrEMBL
Match: A0A6J1CKT2 (uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012216 PE=4 SV=1)

HSP 1 Score: 1235 bits (3196), Expect = 0.0
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 60
           LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF
Sbjct: 23  LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 82

Query: 61  FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD 120
           FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD
Sbjct: 83  FGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLD 142

Query: 121 FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI 180
           FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI
Sbjct: 143 FLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQI 202

Query: 181 NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV 240
           NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV
Sbjct: 203 NVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV 262

Query: 241 IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT 300
           IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT
Sbjct: 263 IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT 322

Query: 301 LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR 360
           LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR
Sbjct: 323 LILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRCGWSVKKYRWSNSVR 382

Query: 361 QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK 420
           QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK
Sbjct: 383 QYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEVNEENRK 442

Query: 421 LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD 480
           LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD
Sbjct: 443 LPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGTLLSD 502

Query: 481 FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD 540
           FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD
Sbjct: 503 FLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKD 562

Query: 541 AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT 600
           AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT
Sbjct: 563 AGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLT 622

Query: 601 HVWLLMHHLG 610
           HVWLLMHHLG
Sbjct: 623 HVWLLMHHLG 632

BLAST of MC06g1081 vs. ExPASy TrEMBL
Match: A0A5D3BS41 (DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00060 PE=4 SV=1)

HSP 1 Score: 473 bits (1217), Expect = 2.71e-156
Identity = 291/622 (46.78%), Postives = 383/622 (61.58%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS--YEDA--- 64
           S  +Q++L+PMGL+RK+SS+  LR  LL+ Y  +DW A  S   LV+ YG+  Y+D    
Sbjct: 24  SLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  -FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 124
            +  R F LA F+LLHLGGSDTITAYSMEDN+LW R+       +  + YI L AL P  
Sbjct: 84  NYIIRAF-LAHFLLLHLGGSDTITAYSMEDNELWLRTLLSLLAVLAASIYIFLQALLPTS 143

Query: 125 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPI 184
           L+++ IP+ +AGIIK  E+IW  RS S +RL D L  +T  SPI          H E  +
Sbjct: 144 LNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEV 203

Query: 185 QINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREA 244
           Q   D E           +LHIAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F S  A
Sbjct: 204 Q---DFE-----------MLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLSYYEKFQSNSA 263

Query: 245 FKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTN 304
           FK+IELELGFMYDFFYTKASI HS  G + RLTT  S+ + I+T+ +      + +   N
Sbjct: 264 FKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLID-KQEYPSTYVN 323

Query: 305 ILTLILLYGALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
           ++ L L +GALS+EI S  L L SDWNVI L  T+S S  L  L  K IS CGWS KK R
Sbjct: 324 LIFL-LFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRY-CKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLE 424
            SNS+ QYNLIS CLK+     Y CK+  T  ++   +  R IS+ L+  IFQQL +KL 
Sbjct: 384 CSNSISQYNLISHCLKQKNDDSYYCKFHNTKTMAAF-SVQRPISNNLEAHIFQQLKKKLV 443

Query: 425 VNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYH-----RDKETEASN 484
           +N+E            + YN++GWSL+LD DQSILLWHIAT+ CY+     ++ E  + +
Sbjct: 444 LNQEY----------DSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSES 503

Query: 485 CSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKS 544
           C   +D   LS+FL Y +V+H SLF  GMS+IR   T +  +E +Q +K     S+  K+
Sbjct: 504 CIPPQDSISLSNFLAYFIVHHPSLFPSGMSQIRHKATSEDVLELLQDKKLGRCNSNMLKN 563

Query: 545 MLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEH 604
            L+L+   V ++   S+    CRLA  L+ LE  ++WEII +VWVE+L  ISCE  WY+H
Sbjct: 564 -LELKIEVVKEERKESMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDH 607

Query: 605 AKKLRHGGNLLTHVWLLMHHLG 610
           AK+L  GGNL+T VW+LMHHLG
Sbjct: 624 AKQLTQGGNLVTRVWILMHHLG 607

BLAST of MC06g1081 vs. ExPASy TrEMBL
Match: A0A0A0LZZ2 (DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=4 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 6.25e-154
Identity = 290/620 (46.77%), Postives = 378/620 (60.97%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS-----YEDA 64
           S  +Q++L+P+GL+RK SS+  LR  LL+ Y  +DW A  S   LV+ YG+     + D 
Sbjct: 24  SLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHL 124
            +     LA F+LLHLGGSDTITAYSMEDN+LW R+       +  + YI L AL P  L
Sbjct: 84  MYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAILAASIYIFLQALLPTSL 143

Query: 125 DFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQ 184
           +++ IP+ +AG+IK  E+IW  RS S +RL D L  +T  SPI          H E  +Q
Sbjct: 144 NYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEVQ 203

Query: 185 INVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREAF 244
              D E           +L IAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F+S+ AF
Sbjct: 204 ---DFE-----------VLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 263

Query: 245 KVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNI 304
           K+IELELGFMYDFFYTKASI HS  G + RLTT  S+V+  +T+ +   D     P T +
Sbjct: 264 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCL--IDKQ-EYPSTYV 323

Query: 305 LTLILLY-GALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
             + LL+ GALS+EI S  LFL SDWNVI L  T+S S  L  L  K IS CGWS+KK R
Sbjct: 324 NLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRYCKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLEV 424
            SNS+ QYNLIS CL++     Y K+  T  I+   +  R IS+ L+  IFQQL QKL +
Sbjct: 384 CSNSISQYNLISHCLEQKNDSYYFKFPSTKTIAAF-SVQRPISNNLEAHIFQQLKQKLVL 443

Query: 425 NEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYHRD---KETEAS-NCS 484
           N+E              YN++GWSL+LD DQSIL+WHIAT+ CYH     KE+E S +C 
Sbjct: 444 NQEY----------DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCI 503

Query: 485 LLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKSML 544
             +D   LS+FL Y +V+H SLF  GMS+IR   T +  +E +Q  K     S+  K+ L
Sbjct: 504 PPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKN-L 563

Query: 545 DLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEHAK 604
           +L    V ++   S      RLA  L+ LE  ++WEII +VWVE+L  ISCEC WY+HAK
Sbjct: 564 ELNIEVVKEERKESRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRISCECEWYDHAK 605

Query: 605 KLRHGGNLLTHVWLLMHHLG 610
           +L  GG+L+T VW+LMHHLG
Sbjct: 624 QLTQGGSLVTRVWILMHHLG 605

BLAST of MC06g1081 vs. ExPASy TrEMBL
Match: A0A1S3C8L7 (uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=4 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 1.09e-152
Identity = 288/622 (46.30%), Postives = 379/622 (60.93%), Query Frame = 0

Query: 5   SFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS--YEDA--- 64
           S  +Q++L+PMGL+RK+SS+   R  LL+ Y  +DW A  S   LV+ YG+  Y+D    
Sbjct: 24  SLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWIANFSFVMLVERYGTGCYDDFTDP 83

Query: 65  -FFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 124
            +  R F LA F+LLHLGG DTITAYSMEDN+LW R+       +  + YI L AL P  
Sbjct: 84  NYIIRAF-LAHFLLLHLGGFDTITAYSMEDNELWLRTLLSLLAVLAASIYIFLQALLPTS 143

Query: 125 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPI 184
           L+++ IP+ +AGIIK  E+IW  RS S +RL D L  +T  SPI          H E  +
Sbjct: 144 LNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTP-SPITT--------HNEEEV 203

Query: 185 QINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQS-LHYFMQFDSREA 244
           Q   D E           +LHIAYYFF  +K LFV L  TSYDLQQ+ L Y+ +F S  A
Sbjct: 204 Q---DFE-----------MLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLCYYEKFQSNSA 263

Query: 245 FKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTN 304
           FK+IELELGFMYDFFYTKASI HS  G + RLTT  S+ + I+T+ +      + +   N
Sbjct: 264 FKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLID-KQEYPSTYVN 323

Query: 305 ILTLILLYGALSLEISSFILFLCSDWNVIRL--TKS-SYSLAHLTFKAISRCGWSVKKYR 364
           ++ L L +GALS+EI S  L L SDWNVI L  T+S S  L  L  K IS CGWS KK R
Sbjct: 324 LIFL-LFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRR 383

Query: 365 WSNSVRQYNLISCCLKETKYGRY-CKYFRTSYISKIMTASRNISDELKTRIFQQLTQKLE 424
            SNS+ QYNLIS CLK+     Y CK+  T  ++   +  R IS+ L+  IFQQL +KL 
Sbjct: 384 CSNSISQYNLISHCLKQKNDDSYYCKFHNTKTMAAF-SVQRPISNNLEAHIFQQLKKKLV 443

Query: 425 VNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATNICYH-----RDKETEASN 484
           +N+E            + YN++GWSL+LD DQSILLWHIAT+ CY+     ++ E  + +
Sbjct: 444 LNQEY----------DSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSES 503

Query: 485 CSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQRKSIETTSDACKS 544
           C   +D   LS+FL Y +V+H SLF   MS+IR   T +  +E +Q +K     S+  K+
Sbjct: 504 CIPPQDSISLSNFLAYFIVHHPSLFPSRMSQIRHKATSEDVLELLQDKKLGRCNSNMLKN 563

Query: 545 MLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCERWEIINHVWVEMLANISCECRWYEH 604
            L+L+   V ++   S+    CRLA  L+ LE  ++WEII +VWVE+L  ISCE  WY+H
Sbjct: 564 -LELKIEVVKEERKESMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDH 607

Query: 605 AKKLRHGGNLLTHVWLLMHHLG 610
           AK L  GGNL+T VW+LMHHLG
Sbjct: 624 AKHLTQGGNLVTRVWILMHHLG 607

BLAST of MC06g1081 vs. ExPASy TrEMBL
Match: A0A6J0ZWT9 (uncharacterized protein LOC110412747 OS=Herrania umbratica OX=108875 GN=LOC110412747 PE=4 SV=1)

HSP 1 Score: 433 bits (1113), Expect = 2.29e-140
Identity = 266/646 (41.18%), Postives = 377/646 (58.36%), Query Frame = 0

Query: 1   LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAF 60
           L + S +LQ+IL   G RRKY + N +  +L L YLSAD+ AT SLG L    G  +D  
Sbjct: 20  LVLLSLSLQIILSFFGNRRKYIARNWISFILWLAYLSADYVATVSLGILASNQGDLDDDS 79

Query: 61  FGRLFIL--APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQH 120
                +   APF+LLHLGG DTITAYS+EDN+LW R   G   QVG+AFY+ + +L+P  
Sbjct: 80  LDNAIMSFWAPFLLLHLGGPDTITAYSLEDNELWLRHLLGLVTQVGVAFYVFIRSLKPMK 139

Query: 121 LDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLE--F 180
           L+F+ IP+F+AG +KYGER WV RS S+Q   + LL      P      +   L     F
Sbjct: 140 LNFVAIPMFIAGTVKYGERTWVLRSASSQHFRESLLQRPDPGPNYAKFMEDYKLKEREGF 199

Query: 181 PIQINVDHEATQNPHFSHLH---------LLHIAYYFFKTNKFLFVDLTLTSYDLQQSLH 240
            +    +   T  P  +            +L  AY FF T K LF DL L+ +D+++S  
Sbjct: 200 ELSCTANQAFTVVPRTNSFQGGSLIPDGAILIAAYDFFNTCKRLFADLILSFHDIEKSHS 259

Query: 241 YFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFH-IDH 300
           YF +    EAFKVIE+ELGF+YD FYTKASI++S WG  LR  +L S ++ + TF  ID 
Sbjct: 260 YFQRCHWDEAFKVIEVELGFVYDLFYTKASIVYSNWGVFLRSVSLSSTIITLATFTAIDR 319

Query: 301 FDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTFKAISRC 360
              GF     +I T +LL GA+ LEI + ++ L S+W ++RL+K        T KAIS  
Sbjct: 320 --QGFKTVDVSI-TFLLLIGAIFLEIYAILVLLSSEWTMLRLSKREKFPVSQTSKAISTF 379

Query: 361 GWSVKKYRWSNSVRQYNLISCCLKETKYGRYCKYFRTSYISKIM---TASRNISDELKTR 420
            +   K RWSN + QY+LI  C +    G      R  +I K +     S ++S  LK  
Sbjct: 380 KFITSKNRWSNYMEQYSLIGSCFRGEP-GDLHGVQRRGWIHKHVHGNITSESVSPCLKEF 439

Query: 421 IFQQLTQKLEVNEE---NRKLPGW----ILRKHNCYNQLGWSLELDSDQSILLWHIATNI 480
           +F++L +K +V      +R+L       +L + NC ++LGWS+E++ D SILLWHIAT++
Sbjct: 440 VFEELVEKSKVASNFSISRQLCACRGEQVLGEKNCLDRLGWSVEVEFDHSILLWHIATSL 499

Query: 481 CYHRDKETEASNC--SLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQ 540
           CY  D++   S+   S  +   L+S++L Y+LV   S+  +G+ +IRF +T+  AIEF+Q
Sbjct: 500 CYCYDQKRNPSSVLDSRCKVSKLISEYLLYILVKRPSMMPNGIGQIRFQDTICEAIEFIQ 559

Query: 541 QRKSIETTSDACKSMLDLET----STVYKDAGNSVFFGGCRLARELQGLEG------CER 600
           +RK I   S AC+ +L ++T    + V  D   SV F  CRLA+EL  +E        E+
Sbjct: 560 ERKFISNASLACEKLLQVDTRIEPAIVKGDRSKSVLFDACRLAKELHSVEQERKWHTNEK 619

Query: 601 WEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLG 610
           WE+++HVW+EML+  + +CRW +HA++LR GG LLTHVWLLM HLG
Sbjct: 620 WELVSHVWLEMLSYAAGQCRWNQHAQQLRRGGELLTHVWLLMAHLG 661

BLAST of MC06g1081 vs. TAIR 10
Match: AT5G45540.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 231.5 bits (589), Expect = 1.8e-60
Identity = 219/769 (28.48%), Postives = 330/769 (42.91%), Query Frame = 0

Query: 3   IFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYEDAFFG 62
           I S  LQ ILI     R+ ++     +L+   YL ADWAA  ++G +       E+A   
Sbjct: 23  ILSLFLQTILIFFAPSRRRTAKKLFLVLIWSAYLLADWAADYAVGQISD--SQEEEAESN 82

Query: 63  R-------LFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQ 122
           +       L   +PF+LLHLGG DTITA ++EDN+LW R  F    Q     Y++LL++ 
Sbjct: 83  KPSKNRELLAFWSPFLLLHLGGPDTITALALEDNELWDRHLFSLVCQAVATVYVILLSI- 142

Query: 123 PQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLST----TRFSPIQINAAKSKH 182
           P  L    + +FV G+IKY ER     S S  +  D +L        ++ +       K 
Sbjct: 143 PNRLLTPTLIMFVGGVIKYVERTAALFSASLDKFKDSMLDDPDPGANYAKLMEEYEARKK 202

Query: 183 LHLEFPIQINVDHE--------ATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQ 242
           +++   + +  D E           +   + L ++  AY +F   K L VDL  T+ +  
Sbjct: 203 MNMPTDVIVVKDPEKGREGNTPVRPDNELTALQVIQYAYKYFNIFKGLIVDLIFTNQERD 262

Query: 243 QSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFH 302
           +S  +F +  + EA ++IE+ELG +YD  +TKA I+H+  G + R   L  +V  +  F 
Sbjct: 263 ESRKFFDKLTAEEALRIIEVELGLIYDCLFTKAEILHNWTGAVFRFIALGCLVASLCLFK 322

Query: 303 IDHFD--PGFNNPLTNILTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAH---- 362
           ++  D   GF+     +LT  LL   ++L+  + ++F  SDW + RL K    L      
Sbjct: 323 MNKKDQYDGFD----VVLTYALLICGIALDSIALLMFCVSDWTIARLRKLKEDLEEKDTL 382

Query: 363 --------LTFKAI----SRCGWSVKKY--------RWSNSVRQYNLISCCL----KETK 422
                   L FK +    S+C     +         RWS  V  YNLI  CL    K   
Sbjct: 383 TDRVLNWILDFKTLRWKRSKCSQDGHQVLNRNFMFRRWSEYVHAYNLIGFCLGIRPKRIH 442

Query: 423 Y--GRYCKYF-RTSYISKIMTASRN----------------------------------- 482
           Y  G+   +F +T +I  I TA  N                                   
Sbjct: 443 YTKGKIHSFFHQTVHILSIDTAIENATRGTRQFHNWIGRFLSNLSKRDNSVIRTGLRWFL 502

Query: 483 -----------------------------ISD----ELKTRIFQQLTQKLEVNEENRKLP 542
                                        +SD    EL   IF ++ QK    E+     
Sbjct: 503 FFPQLLGLLIYNFLDFFGIKDLVEEIRFTVSDRLTRELWEFIFTEVQQKHRFAEDQESAK 562

Query: 543 G-------WILRKHNCYNQ---------LGWSLELDSDQSILLWHIATNICYHR------ 602
           G       W L + +   +         L +  E D DQSILLWHIAT + Y +      
Sbjct: 563 GISSARGNWTLLETSSKKKEDGTDHTKLLQYVTEKDYDQSILLWHIATELLYQKPIDKKV 622

Query: 603 -DKETEASN-----CSLLEDGTLLSDFLTYLLVYHHSLF--LDGMSEIRFCETVDSAIEF 611
            +KE  ++N      S  E   +LSD++ YLL+   +L   + G+++IRF +T + A +F
Sbjct: 623 TEKEEHSTNREKEEHSNREFSKILSDYMMYLLIVQPTLMSAVSGIAKIRFRDTCEEAKDF 682

BLAST of MC06g1081 vs. TAIR 10
Match: AT5G45530.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 208.0 bits (528), Expect = 2.2e-53
Identity = 208/764 (27.23%), Postives = 319/764 (41.75%), Query Frame = 0

Query: 1   LPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGSYED-- 60
           L I S   Q  LI +   RK +S   L  +L   YL ADW A  ++  + K  G   +  
Sbjct: 20  LVIMSLLFQTSLIFLAPMRKRTSKKLLAAVLWTAYLLADWTANYAVSQITKNQGKETEPG 79

Query: 61  ---AFFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQ 120
                   L + APF+LLHLGG DTITA ++EDN LW R  FG   Q     Y ++ +L+
Sbjct: 80  DPPKNKKLLALWAPFLLLHLGGPDTITALALEDNALWQRHLFGLVSQALAGVYAVVQSLE 139

Query: 121 PQHLDFLGIPI---FVAGIIKYGERIWVFRSTSTQRLPDLLL----STTRFSPIQINAAK 180
               + L  PI   F+ G IKY ER     S S  +  D +L    + + ++ +    A 
Sbjct: 140 ----NVLWPPITLLFITGTIKYVERTRALYSASLDKFKDRMLQRADAGSNYAKLMEEFAS 199

Query: 181 SKHLHLEFPIQINVDHEATQNP--------HFSHLHLLHIAYYFFKTNKFLFVDLTLTSY 240
            K  +L   I +  + +  + P          + L ++   + FF T K L VDL  +  
Sbjct: 200 RKMSNLPTEIFLTDEPDKHERPPTLVKPDRDLTDLEIVQYGFKFFNTFKGLVVDLIFSFR 259

Query: 241 DLQQSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIV 300
           +  +S  +F +    EA ++IE ELGF+Y+  YTK +I+H+  G + RL +  S++    
Sbjct: 260 ERDESRDFFKELKPGEALRIIETELGFLYESMYTKTAILHTGIGTLFRLISFGSLLSSFF 319

Query: 301 TFHIDHFDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWN--VIRLTK-----SSY 360
            FH         +    ++T +L    ++L+++S ++FL SDW   V+R  K      S 
Sbjct: 320 VFHRRPLKSEDFHGADVVITYVLFIVGIALDLASMVIFLLSDWTFAVLRNLKDDPEEKST 379

Query: 361 SLAHL--TFKAISRCGWSVKKY------------------RWSNSVRQYNLISCCLKE-- 420
           S+  L   F    +  W  KK+                  RWS ++  +N I  CLK   
Sbjct: 380 SIDSLFNWFLEFRKPRW--KKHTCNGNQTHEVLSTGFFTRRWSGTIYGFNFIGFCLKAKV 439

Query: 421 ----------------------------------------------------------TK 480
                                                                     T 
Sbjct: 440 SRIHQKRNCNLLVWDYVVSLFDLVIRRIQMMIGWIKNVNRSIRSVLRQWSKKNPMIRCTV 499

Query: 481 YGRYCKYFR---------TSYISKIMTAS------RNISDELKTR-----IFQQLTQK-- 540
           Y  Y  +F            YI +I + +      R IS E  T+     IF ++  K  
Sbjct: 500 YPLYLVFFAGIPEVFRVLWKYIDRIFSVTSYLDGIRFISREPLTKNQWEFIFNEVKDKSG 559

Query: 541 -LEVNEENRKLP----GWILRKHNCY---NQLGWSLELDSDQSILLWHIATNICYHRDKE 600
             E  E  +K+      W LR          + +  ++D DQS+LLWHIAT +C+ +++ 
Sbjct: 560 FAETPEVAKKVSWARGEWALRDSKLMEVDTLMRYIEKVDYDQSLLLWHIATELCFQKEEG 619

Query: 601 TEASNCSL-----LEDGTLLSDFLTYLLVYHHSLFLD--GMSEIRFCETVDSAIEFMQQR 611
            +    S       E   ++SD++ YLL+    L  +  G+  IRF +T   A  F + R
Sbjct: 620 GKMEKLSREGYDDREFSKIISDYMMYLLIMRPKLMSEVAGIGTIRFRDTKAEAERFFKGR 679

BLAST of MC06g1081 vs. TAIR 10
Match: AT5G45480.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 196.8 bits (499), Expect = 5.0e-50
Identity = 220/848 (25.94%), Postives = 319/848 (37.62%), Query Frame = 0

Query: 3   IFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYGS----YED 62
           IFS +LQ  LI    +RK SS   L   +   YL ADW+A  + G +    G      E 
Sbjct: 23  IFSLSLQTFLIFFAPQRKRSSRKVLLSFIWSAYLLADWSANFAAGQISDSQGDDPEPGEP 82

Query: 63  AFFGRLFIL-APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQ 122
                LF    PF+LLHLGG DTITA ++EDN+LW R   G F Q     Y+LL +L P 
Sbjct: 83  KKSAELFAFWVPFLLLHLGGPDTITALALEDNELWLRHLLGLFFQSVATVYVLLQSL-PN 142

Query: 123 HLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSP-----IQINAAKSKHL 182
            L    + +F  G+IKY ER       S  +  D ++      P     ++  AAK    
Sbjct: 143 ALWKPILLVFATGVIKYVERTLALYLASLDKFKDSMIQRPDPGPNYAKLMEEYAAKK--- 202

Query: 183 HLEFPIQINVDHEATQNPH----------FSHLHLLHIAYYFFKTNKFLFVDLTLTSYDL 242
            ++ P QI    E  ++P           F+ L++L  AY +F   K L VDL  T    
Sbjct: 203 DMKMPTQIIKVGEPEKDPRDDAPVKPPDGFTPLNILQYAYKYFNIFKGLVVDLIFTFQQR 262

Query: 243 QQSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTF 302
            +S  +F    + EA +++E+EL F+Y   YTKA I+H+  G + R   L  +   +  F
Sbjct: 263 AESKRFFDSLKAEEALRILEVELNFIYAALYTKAEILHNWIGFLFRFIALGCLAAALRIF 322

Query: 303 H------IDHFDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWNVIRLTK------ 362
                     FD G        LT  LL G ++L+  + I+F  SDW  +RL K      
Sbjct: 323 QYKSKKDYSGFDVG--------LTYALLLGGIALDCIALIMFCASDWTFVRLRKMKDEVD 382

Query: 363 ---------------SSYSLAHLTFKAISRC----------GWSVKK------------- 422
                          +   +  L  +    C            S KK             
Sbjct: 383 DPPTWSDNILNWILENILGVRKLKVEEYDECYKNTQSHEVPNTSTKKTPFLKRILNRILR 442

Query: 423 ---------------------------------------------YRWSNSVRQYNLISC 482
                                                         RWS  V  +NLI  
Sbjct: 443 VRELKTEKSHEVLDKSTSKIPGQEVPDKSAKTIPCHKVLDTSFMYRRWSEYVHAHNLIEY 502

Query: 483 CLK-------------------------------------------------------ET 542
           CL                                                        + 
Sbjct: 503 CLGLKPKRIHHTKGFIHIAFDKVINILYIGPAFTKLGSVIESCFRVTKQRIHQTFKWIDG 562

Query: 543 KYGRYCK--------YFRTSYI---------------------------SKIMTASRNIS 602
           K  R CK        Y R S+                              I T+S  ++
Sbjct: 563 KVSRLCKKYPKLNEEYIRFSFFCIFYIPSLPGRWIKSFMEFFGIRAQLDEVIYTSSDRLT 622

Query: 603 DELKTRIFQQLTQKLEVNEENRKL-------PGWILR--------KHNCYNQLGWSLELD 611
            ++   IF ++  K    +++            W LR        +      L + +E+D
Sbjct: 623 LDMWEHIFGEVKAKSRFADDSESAMRVSSARGDWTLRDIQGDPETEKKREKLLRYVMEMD 682

BLAST of MC06g1081 vs. TAIR 10
Match: AT5G45470.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 192.6 bits (488), Expect = 9.4e-49
Identity = 215/831 (25.87%), Postives = 337/831 (40.55%), Query Frame = 0

Query: 3   IFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGTLVKFYG---SYEDA 62
           I S TLQ ILI     RK +    L +L+   YL ADW+A  ++G + K  G     +D 
Sbjct: 23  ILSLTLQAILICFSPLRKRTPRRLLIVLVWSSYLLADWSANFAVGLISKNQGKDLKPDDP 82

Query: 63  FFGR--LFILAPFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQ 122
              +  + + APF+LLHLGG DTITA+++EDN LW R  FG   Q     Y+++++L P 
Sbjct: 83  PQDKKVMALWAPFLLLHLGGPDTITAFALEDNALWLRHVFGLVFQAIAGVYVVVMSL-PN 142

Query: 123 HLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAK--------- 182
            L  + + +FV+G IKY ER     S S  +  D ++      P   N AK         
Sbjct: 143 SLWVVIVLVFVSGTIKYLERTTALYSASLDKFRDSMIQAPDPGP---NYAKLMEEYKAKK 202

Query: 183 -----SKHLHLEFPIQIN----VDHEA----TQNPHFSHLHLLHIAYYFFKTNKFLFVDL 242
                +K + ++ P + N    ++H A     +    + L ++  AY FF T K L V+L
Sbjct: 203 EARLPTKIVLIDEPDKENRPKKLEHPALASKKRKKDLTDLEIVQYAYKFFNTFKGLVVNL 262

Query: 243 TLTSYDLQQSLHYFMQF-DSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFS 302
             +  +  +SL  F    D  EA ++IE+ELGF+YD  +TK +I+H+  G + R+    +
Sbjct: 263 IFSFRERDESLEIFENLNDPEEALRIIEIELGFLYDALFTKIAILHTGIGTVSRVFASGT 322

Query: 303 IVVVIVTFHIDHFDPGFNNPLTNILTLILLYGALSLEISSFILFLCSDWN---------- 362
           +V   + FH         +    ++T  L    L L+  S +LFL SDW           
Sbjct: 323 LVAAFIIFHKKPNKGTDFHGADVVVTYTLFAVGLVLDFISILLFLFSDWTCAAYSSLKDD 382

Query: 363 --------------VIRLTKSSYSLAHLTFKAISRC-----------------------G 422
                         +++  K  ++        + +C                       G
Sbjct: 383 PDELQSWQERCFNWLLKFRKLRWTPQECHKTGMHKCTKEGLKPCIMKGADKKEGGNKNEG 442

Query: 423 WSVKK--------------------------YRWSNSVRQYNLISCCLK---------ET 482
            + K+                           RWS S+  +N I+   K           
Sbjct: 443 GNKKEGDDKKEAADKCSLVDKHDVLTTRFFLRRWSGSINVFNFIAYATKADVERIHDARG 502

Query: 483 KYGRY---------------CKYFRTSYISKIMTASRNISDELK---------------- 542
            + RY                K    S +  I    R IS E+                 
Sbjct: 503 SFRRYSWKIITFPFKKLNFVIKKIFGSIVKLINEVHRRISHEVNALSRKHLWARRFLYPI 562

Query: 543 -------------------------------------------TR-----IFQQLTQKLE 602
                                                      TR     IF++L  K +
Sbjct: 563 YFEFISRIPHFIKSVWDILSEFFDISDTLDMVHKTLFVHGEPMTRELWKFIFEELKNKSK 622

Query: 603 VNE--ENRKLPG-----WILRKH-----NCYNQLGWSLELDSDQSILLWHIATNICYHR- 611
             +  EN K        W LR++          + +  ++D DQS+L+WHIAT +CY + 
Sbjct: 623 YGDSPENAKRISLARGEWTLRENLPVDAEREKLVRYVTKVDYDQSLLMWHIATELCYQQH 682

BLAST of MC06g1081 vs. TAIR 10
Match: AT4G19090.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 158.3 bits (399), Expect = 2.0e-38
Identity = 164/678 (24.19%), Postives = 278/678 (41.00%), Query Frame = 0

Query: 68  APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQVGIAFYILLLALQPQHLDFLGIPIF 127
           APF+LLHLGG DTITA+S+EDN LW R F G   Q     Y+++ +L P  L  + + +F
Sbjct: 87  APFLLLHLGGPDTITAFSLEDNALWNRHFLGLVFQALAGVYVVVQSL-PNVLSVIILLLF 146

Query: 128 VAGIIKYGERIWVFRSTSTQRLPDLLLSTTRFSPIQINAAKSKHLHLEFPIQINVDHEAT 187
           +AG  KY ER       S+ +  + +L  +       +  +   +  +   ++N+     
Sbjct: 147 IAGTSKYLERTIALYLASSDKYRNSMLQASNSRFDYTDQTRDLDMDTKLASEMNMKEHRG 206

Query: 188 Q-------NPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYDLQQSLHYFMQFDSREAFKV 247
           Q        PH    HL  + Y FF           L   D  ++    +Q    EAF +
Sbjct: 207 QPKPLKLLQPHKELTHLEILQYAFF-----------LELRDESKAFFSALQLKD-EAFCI 266

Query: 248 IELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVTFHIDHFDPGFNNPLTNILT 307
           IE EL F+Y+  YTK S++HS  G + R  +L S++     +H  H      +    ++T
Sbjct: 267 IEAELDFIYEGLYTKGSVLHSWVGLVSRFISLGSLLSAFTIYHYRHNKIQEFHKADIVIT 326

Query: 308 LILLYGALSLEISSFILFLCSDWNVIRLTK-----------SSYSLAHLTFKAISRCGWS 367
             L    ++L++ S  +F+ SDW    L K             + L  + F    +  W 
Sbjct: 327 YTLFLVGIALDVISIHMFMVSDWTTAILAKLKDDPDERYSGKDHILNWILFLKRPKWKWQ 386

Query: 368 VKK--------------YRWSNSVRQYNLISCCLK------------------------- 427
             +               RW+ S+   N ++  +K                         
Sbjct: 387 TCREGDQQEVLNTPFLLRRWTGSITMLNFLTYSMKADTERIHDPRSRACQLLWKIAVCPF 446

Query: 428 -------ETKYGRYCKYFRT--------------------------------------SY 487
                  +T +    K+F                                        ++
Sbjct: 447 SFIFSIIQTWFSNIAKWFCALHWYIIHHVIILPPGQNQVARSIIGIFKAFVEFWFNVPNF 506

Query: 488 ISKIMTASRN---ISDEL-KTRIFQQLTQKL----EVNEENRKLPGWI-------LRKHN 547
              ++T+  N   I+D   + R+ Q+ +Q L        ++     W        +  H+
Sbjct: 507 FVYLVTSVINFLGINDLFYEIRLLQKNSQILYSLENTTTKSSARRDWASSHTQIQIADHD 566

Query: 548 CYNQLGWSLELDSDQSILLWHIATNICYHRDKETEASNCSLLEDGT------LLSDFLTY 607
               L +  ++D + S+L+WHIAT +CY +++++   NC   E  T      ++SD++ Y
Sbjct: 567 ML--LHYITDVDYEHSLLIWHIATELCY-QEEDSAKENCDKSEYHTNRKISKIISDYMMY 626

Query: 608 LLVYHHSLFLD--GMSEIRFCETVDSAIEFMQQRKSIETTSD---ACKSMLDLETSTVYK 611
           LL+    L  +  G+ +IRF +T+  A  F ++   I  + +   A K +L  +TS   +
Sbjct: 627 LLIMQPKLMSEVAGIGKIRFRDTLAEADRFFKKMGIIRDSRNVKLASKEILSADTSIEPR 686

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022141971.10.0100.00uncharacterized protein LOC111012216 [Momordica charantia][more]
KAA0037446.15.59e-15646.78uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK0... [more]
XP_004139148.11.29e-15346.77uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical ... [more]
XP_008458716.12.25e-15246.30PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo][more]
XP_021279036.14.74e-14041.18uncharacterized protein LOC110412747 [Herrania umbratica][more]
Match NameE-valueIdentityDescription
A0A6J1CKT20.0100.00uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A5D3BS412.71e-15646.78DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0LZZ26.25e-15446.77DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=... [more]
A0A1S3C8L71.09e-15246.30uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=... [more]
A0A6J0ZWT92.29e-14041.18uncharacterized protein LOC110412747 OS=Herrania umbratica OX=108875 GN=LOC11041... [more]
Match NameE-valueIdentityDescription
AT5G45540.11.8e-6028.48Protein of unknown function (DUF594) [more]
AT5G45530.12.2e-5327.23Protein of unknown function (DUF594) [more]
AT5G45480.15.0e-5025.94Protein of unknown function (DUF594) [more]
AT5G45470.19.4e-4925.87Protein of unknown function (DUF594) [more]
AT4G19090.12.0e-3824.19Protein of unknown function (DUF594) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025315Domain of unknown function DUF4220PFAMPF13968DUF4220coord: 33..366
e-value: 1.2E-64
score: 218.9
IPR007658Protein of unknown function DUF594PFAMPF04578DUF594coord: 563..610
e-value: 8.5E-19
score: 66.9
NoneNo IPR availablePANTHERPTHR31325:SF207OS08G0149333 PROTEINcoord: 4..607
NoneNo IPR availablePANTHERPTHR31325OS01G0798800 PROTEIN-RELATEDcoord: 4..607

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1081.1MC06g1081.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane