MC06g0904 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0904
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionL-ascorbate oxidase
LocationMC06: 7598362 .. 7598606 (-)
RNA-Seq ExpressionMC06g0904
SyntenyMC06g0904
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAGGGTACATGTTATCGTCTCTAGATTGCATAGCGAATATGGTTATGGTGATCAATAAAAAGTTCCCTAGTATGACCATTAGAGATGACGTTGGAGATAGTATCGTCGTCAACATCACCAACAAATTTTTCACCGAAGACGTCATCATTCACTTGCATGGCATGAGGCAATTGGAATTCTGTGGGTTGATGGCATGGCCTCCATCTTGCAGTGTGCCATCAACCTCAGAGAAACCTTCAATTAC

mRNA sequence

GTAGGGTACATGTTATCGTCTCTAGATTGCATAGCGAATATGGTTATGGTGATCAATAAAAAGTTCCCTAGTATGACCATTAGAGATGACGTTGGAGATAGTATCGTCGTCAACATCACCAACAAATTTTTCACCGAAGACGTCATCATTCACTTGCATGGCATGAGGCAAGGAATTCTGTGGGTTGATGGCATGGCCTCCATCTTGCAGTGTGCCATCAACCTCAGAGAAACCTTCAATTAC

Coding sequence (CDS)

GTAGGGTACATGTTATCGTCTCTAGATTGCATAGCGAATATGGTTATGGTGATCAATAAAAAGTTCCCTAGTATGACCATTAGAGATGACGTTGGAGATAGTATCGTCGTCAACATCACCAACAAATTTTTCACCGAAGACGTCATCATTCACTTGCATGGCATGAGGCAAGGAATTCTGTGGGTTGATGGCATGGCCTCCATCTTGCAGTGTGCCATCAACCTCAGAGAAACCTTCAATTAC

Protein sequence

VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQGILWVDGMASILQCAINLRETFNY
Homology
BLAST of MC06g0904 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-18
Identity = 46/82 (56.10%), Postives = 56/82 (68.29%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHG-MRQGI 60
           V YM  S DC+ N+VM IN +FP  TIR + GD +VV +TNK  TE V+IH HG +++G 
Sbjct: 45  VEYMFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGT 104

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG ASI QCAIN  ETF Y
Sbjct: 105 PWADGTASISQCAINPGETFTY 126

BLAST of MC06g0904 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.0e-16
Identity = 45/82 (54.88%), Postives = 56/82 (68.29%), Query Frame = 0

Query: 1  VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHG-MRQGI 60
          V YM  + +C  N+VM IN +FP  TIR + GDS+VV +TNK  TE V+IH HG +++G 
Sbjct: 10 VEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGILQRGT 69

Query: 61 LWVDGMASILQCAINLRETFNY 82
           W DG ASI QCAIN  ETF Y
Sbjct: 70 PWADGTASISQCAINPGETFFY 91

BLAST of MC06g0904 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 3.0e-16
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHG-MRQGI 60
           V YM  + DC  N+VM IN +FP  TIR + GD++VV + NK  TE V+IH HG +++G 
Sbjct: 40  VEYMFWAPDCNENIVMGINGQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGT 99

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG ASI QCAIN  ETF Y
Sbjct: 100 PWADGTASISQCAINPGETFFY 121

BLAST of MC06g0904 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 9.7e-15
Identity = 41/82 (50.00%), Postives = 51/82 (62.20%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V Y     DC   MVM +N +FP  TI    GD+IVV++TNK  TE ++IH HG+RQ G 
Sbjct: 27  VEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVIHWHGIRQLGS 86

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG A + QCAI+  ETF Y
Sbjct: 87  PWADGAAGVTQCAISPGETFTY 108

BLAST of MC06g0904 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.2e-14
Identity = 43/82 (52.44%), Postives = 54/82 (65.85%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V Y+  S D   ++VM IN +FP  TIR   GD++ V++TNK  TE V+IH HG+RQ G 
Sbjct: 37  VEYIHWSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGT 96

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG A+I QCAIN  ETF Y
Sbjct: 97  PWADGTAAISQCAINPGETFLY 118

BLAST of MC06g0904 vs. NCBI nr
Match: XP_028758119.1 (L-ascorbate oxidase-like [Prosopis alba])

HSP 1 Score: 98.2 bits (243), Expect = 1.23e-21
Identity = 51/82 (62.20%), Postives = 59/82 (71.95%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM +S DCI N+VM IN +FP  TIR  VGD++VV +TNK  TE V+IH HG+RQ G 
Sbjct: 32  VEYMYASPDCIENVVMGINGQFPGPTIRAQVGDTVVVALTNKLSTEGVVIHWHGIRQKGT 91

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETFNY
Sbjct: 92  PWADGTASISQCAINPGETFNY 113

BLAST of MC06g0904 vs. NCBI nr
Match: XP_024019721.1 (L-ascorbate oxidase [Morus notabilis])

HSP 1 Score: 95.1 bits (235), Expect = 1.17e-20
Identity = 48/82 (58.54%), Postives = 59/82 (71.95%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  SLDC+ ++VM IN +FP+ TIR + GD++VV +TNK  TE V+IH HG+RQ G 
Sbjct: 38  VEYMYRSLDCVQHVVMGINGQFPAPTIRANAGDTVVVRLTNKLQTEGVVIHWHGIRQLGT 97

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 98  PWADGTASISQCAINPGETFTY 119

BLAST of MC06g0904 vs. NCBI nr
Match: EXB54661.1 (L-ascorbate oxidase [Morus notabilis])

HSP 1 Score: 95.1 bits (235), Expect = 1.19e-20
Identity = 48/82 (58.54%), Postives = 59/82 (71.95%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  SLDC+ ++VM IN +FP+ TIR + GD++VV +TNK  TE V+IH HG+RQ G 
Sbjct: 38  VEYMYRSLDCVQHVVMGINGQFPAPTIRANAGDTVVVRLTNKLQTEGVVIHWHGIRQLGT 97

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 98  PWADGTASISQCAINPGETFTY 119

BLAST of MC06g0904 vs. NCBI nr
Match: RWR78074.1 (L-ascorbate oxidase-like protein [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 95.1 bits (235), Expect = 1.55e-20
Identity = 49/82 (59.76%), Postives = 57/82 (69.51%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           VGYM  S DC+ N+V+ IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 47  VGYMFWSPDCVENVVIGINGQFPGPTIRAKAGDTIVVELTNKLHTEGVVIHWHGIRQFGT 106

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 107 PWADGTASISQCAINPEETFVY 128

BLAST of MC06g0904 vs. NCBI nr
Match: KAA8550051.1 (hypothetical protein F0562_001735 [Nyssa sinensis])

HSP 1 Score: 90.9 bits (224), Expect = 2.67e-20
Identity = 49/82 (59.76%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  S DCI  +VM IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 47  VEYMYWSPDCIEGVVMGINGQFPGPTIRGHAGDTIVVELTNKLHTEGVVIHWHGIRQLGT 106

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 107 PWADGTASISQCAINPGETFVY 128

BLAST of MC06g0904 vs. ExPASy TrEMBL
Match: A0A2N9GZA2 (Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS32572 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.11e-22
Identity = 48/82 (58.54%), Postives = 57/82 (69.51%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  S DC+ N+VM IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 35  VEYMFWSPDCVENVVMGINGQFPGPTIRAKAGDTIVVELTNKLPTEGVVIHWHGIRQLGT 94

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QC+IN  ETF+Y
Sbjct: 95  PWADGTASISQCSINPGETFHY 116

BLAST of MC06g0904 vs. ExPASy TrEMBL
Match: A0A2N9HSY1 (Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42705 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.35e-21
Identity = 48/82 (58.54%), Postives = 57/82 (69.51%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  S DC+ N+VM IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 33  VEYMFWSPDCVENVVMGINGQFPGPTIRAKAGDTIVVELTNKLPTEGVVIHWHGIRQLGT 92

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QC+IN  ETF+Y
Sbjct: 93  PWADGTASISQCSINPGETFHY 114

BLAST of MC06g0904 vs. ExPASy TrEMBL
Match: A0A2N9FG55 (Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13636 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.37e-21
Identity = 48/82 (58.54%), Postives = 57/82 (69.51%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  S DC+ N+VM IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 49  VEYMFWSPDCVENVVMGINGQFPGPTIRAKAGDTIVVELTNKLPTEGVVIHWHGIRQLGT 108

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QC+IN  ETF+Y
Sbjct: 109 PWADGTASISQCSINPGETFHY 130

BLAST of MC06g0904 vs. ExPASy TrEMBL
Match: W9RGW8 (L-ascorbate oxidase OS=Morus notabilis OX=981085 GN=L484_022522 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 5.75e-21
Identity = 48/82 (58.54%), Postives = 59/82 (71.95%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V YM  SLDC+ ++VM IN +FP+ TIR + GD++VV +TNK  TE V+IH HG+RQ G 
Sbjct: 38  VEYMYRSLDCVQHVVMGINGQFPAPTIRANAGDTVVVRLTNKLQTEGVVIHWHGIRQLGT 97

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 98  PWADGTASISQCAINPGETFTY 119

BLAST of MC06g0904 vs. ExPASy TrEMBL
Match: A0A3S3MKG0 (L-ascorbate oxidase-like protein OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_00658300 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.51e-21
Identity = 49/82 (59.76%), Postives = 57/82 (69.51%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           VGYM  S DC+ N+V+ IN +FP  TIR   GD+IVV +TNK  TE V+IH HG+RQ G 
Sbjct: 47  VGYMFWSPDCVENVVIGINGQFPGPTIRAKAGDTIVVELTNKLHTEGVVIHWHGIRQFGT 106

Query: 61  LWVDGMASILQCAINLRETFNY 81
            W DG ASI QCAIN  ETF Y
Sbjct: 107 PWADGTASISQCAINPEETFVY 128

BLAST of MC06g0904 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 84.3 bits (207), Expect = 4.8e-17
Identity = 43/82 (52.44%), Postives = 52/82 (63.41%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V Y   S DC    VM +N +FP  TI+   GD+IVVN+TNK  TE ++IH HG+RQ G 
Sbjct: 44  VEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGS 103

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG A + QCAIN  ETF Y
Sbjct: 104 PWADGAAGVTQCAINPGETFTY 125

BLAST of MC06g0904 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 84.3 bits (207), Expect = 4.8e-17
Identity = 43/82 (52.44%), Postives = 52/82 (63.41%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V Y   S DC    VM +N +FP  TI+   GD+IVVN+TNK  TE ++IH HG+RQ G 
Sbjct: 44  VEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGS 103

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG A + QCAIN  ETF Y
Sbjct: 104 PWADGAAGVTQCAINPGETFTY 125

BLAST of MC06g0904 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 77.8 bits (190), Expect = 4.5e-15
Identity = 36/74 (48.65%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 9   DCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GILWVDGMAS 68
           DC   +VM IN +FP  TI    GD++++++ NK  TE V+IH HG+RQ G  W DG A 
Sbjct: 38  DCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAG 97

Query: 69  ILQCAINLRETFNY 82
           + QC IN  ETF Y
Sbjct: 98  VTQCPINPGETFTY 111

BLAST of MC06g0904 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 2.2e-14
Identity = 38/82 (46.34%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 1   VGYMLSSLDCIANMVMVINKKFPSMTIRDDVGDSIVVNITNKFFTEDVIIHLHGMRQ-GI 60
           V Y   S DC   +V+ IN KFP  TI+   GD+IVV + N F TE+V +H HG+RQ G 
Sbjct: 42  VKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHGIRQIGT 101

Query: 61  LWVDGMASILQCAINLRETFNY 82
            W DG+  + QC I   E F Y
Sbjct: 102 PWFDGVEGVTQCPILPGEVFIY 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P141335.5e-1856.10L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P370641.0e-1654.88L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
P247923.0e-1653.66L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
M4DUF29.7e-1550.00L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Q405882.2e-1452.44L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_028758119.11.23e-2162.20L-ascorbate oxidase-like [Prosopis alba][more]
XP_024019721.11.17e-2058.54L-ascorbate oxidase [Morus notabilis][more]
EXB54661.11.19e-2058.54L-ascorbate oxidase [Morus notabilis][more]
RWR78074.11.55e-2059.76L-ascorbate oxidase-like protein [Cinnamomum micranthum f. kanehirae][more]
KAA8550051.12.67e-2059.76hypothetical protein F0562_001735 [Nyssa sinensis][more]
Match NameE-valueIdentityDescription
A0A2N9GZA29.11e-2258.54Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L... [more]
A0A2N9HSY12.35e-2158.54Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L... [more]
A0A2N9FG553.37e-2158.54Plastocyanin-like domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L... [more]
W9RGW85.75e-2158.54L-ascorbate oxidase OS=Morus notabilis OX=981085 GN=L484_022522 PE=3 SV=1[more]
A0A3S3MKG07.51e-2159.76L-ascorbate oxidase-like protein OS=Cinnamomum micranthum f. kanehirae OX=337451... [more]
Match NameE-valueIdentityDescription
AT5G21105.14.8e-1752.44Plant L-ascorbate oxidase [more]
AT5G21105.34.8e-1752.44Plant L-ascorbate oxidase [more]
AT5G21100.14.5e-1548.65Plant L-ascorbate oxidase [more]
AT4G39830.12.2e-1446.34Cupredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 7..81
e-value: 1.3E-16
score: 60.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 5..81
e-value: 2.0E-14
score: 56.0
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 4..79
NoneNo IPR availablePANTHERPTHR11709:SF394PLANT L-ASCORBATE OXIDASEcoord: 3..81
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 3..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0904.1MC06g0904.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity