MC06g0866 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0866
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial
LocationMC06: 7209544 .. 7214818 (-)
RNA-Seq ExpressionMC06g0866
SyntenyMC06g0866
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGTGATCAAGGTTTCATGATATTTTATTTCAATCCGGAAGAACATTCATACAATTTTTCAAACTTAATATATAATTTGATAAAGTTCATCTTTTTATTTGATAAAAATAAACTCTATCTTCAAAGTCTTTTCCAACTTTGTCTCTTTGCATAATTAAGAATTCCCATTACAGAGACGCCATTTGTGTGAGAGAGAGAATTTAATGACTATCAGCAGATACATTGGATAATCTCAATGACTTTTCTGAGTTTTCCGCATTGGATTTTATCCGTTTGAGGCGTTTCATCCGGCAGTTACGCAAAATCGATCATCAAATTGCTGAGAAGGGAAGCTCTGTGTTAATTATCTCTTCCAAATGGTAACTATTTTGAGGAGATCGCAGACAGTGTTGAACTCCATTTGCAGGAGGAAATTTTCTTCTTCCTCTGATGGCGATCTTACGAAACCGAGCGGAAAATCCATGAACCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCGCTCGAAACTGATCCTCGGTCTGTTTCTTTCAGAACCCATCTCGTTTTTTGGTTTGTGATGGACTGCAATTTGATTTTCTGCGGAGATACTGTAATCGCATGCTGATTTGAGATACTTCTTGGCTGATTTGTTGTGGGTTTTTCTTATGGGTTTCGTATGTTTAATCGACTTGTTTCTTTCGTGTTCTTTCATTGTTGGTTGCTGGTTGGTTGCCCTTTTTACGAGCCTGTGTTTAGAGTTGGCCAAAGCCAAACACCGTATCAAAGCGAGCATTGGAAAAATAAGAGAACATCACTGAATGAGAGAGTTCAAAATAACAATACCGATGGCTAAGGTAGTGAGAGCTTGCATGGAAATGAAAGTGAAGGAAAATTGATGAAACATGTCACCACATTTTGTAGAAATTGTTTGTGGGCTGGTGTTTTGTTTCCTCGTTCTATCACGAGACGAAGCTTCCTTCGGTTATTAATAGAATTAGGCGAAACCATGTTATGTGAGAAAGGCTATATCTGATTTGATTGGATGAACTCCCATAATTAGATGGTTCTGTGGAACTGTGGAAAATTGCATTGTTCTACAATTCTTAAGGAGTGAATGGAAAGATTCCAGAGATTGTTCATTCTAGGGCCTCCCTTTGGAGTTCTTCTATTATATATACATAGAGGATTGTACTCCCCTTGGCTAATTGAGCTGATTTTTTTATCACCCTTTTGTACTTAACATAGTTTTATGCTCTGGTAGCAATTTTGTTTTAAAAGGACATTATACTTTGCCATTCAGTTCTTATGTATTTGGAGAAGATGTGGGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTGGCAGAACGATACGGGAAGGACCGAGTTTTCAATACCCCTCTTTGCGAGCAGGTAACTTATTTATCCACTTTGAAACTTTGGAGTCCAATATGCGAGCAGAGCATATTAACTTTTCCTTATAATCAAGTATATGTGATAAGTTTCATTTTAACTTTTCCTTCATTCAGGGAATTGTTGGCTTTGGCATTGGTCTGGCAGCAATGGTAATTTTGAGGAATTTGTGCGCTCATTTCATCAGCATGAATTCCAAGCCAGTCATTGGACTTCTTTTTGTATTATACATCTTCTCCCTTTTCTAACACGGGAAATCATTTCGCATGATAGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGGTAAGCTTTCTTCTTCATTGAAAATTTTTCTCTTGCCTCTTGTTTGGCATTATACGAGAAACTTCTACCTTTATAGATTGTTAATGAAGCTGCAAAGTTCAGATACCGTAGTGGAAATCAGTTCAATTGTGGTGGTAAATTTTCTCCTCTCTTTGTATACAGTCTACTTCTTTCGTGTTTTTTCTTGTTATTTGGCCCTTCATGTCGTGCATATTTGTGACTCCTATCAGGTTTAACTATTAGAGCACCATATGGAGCTGTTGGTCATGGTGGTCACTATCACTCACAATCACCAGAAGCTTTCTTTTGTCATGTTCCTGGCATCAAAGTATGCTTCTGTCTTCTTCTGATCTGCTAATTATAGGGTTTTTCAGTCGTGCATTAGCTTCATGATATCAATCCTTGATTCTCTTAGCCGTCAAATTCCTTAGCGGGCATTCGTTTATTTCTTTCTGTTAATAGTTCCCAACAAAAGGCTCTGATCGTATTACACCACATAAAAATTTGAAGAATTTATCACTGTTTCATAGCCATTTTTGTTGAGAATGTACCAACACCAGGAGATATTGATGTGCATCGAGTGATTTGTTATTTTTCAAACGTCATAGCATGAATTGCTATTTTTGAAGAAAACGCAAATATATTTTTTTCAACATCATTCTTGTGCTAAATAGGTTGTCATCCCGCGAAGCCCGTACCAGGCAAAAGGATTGTTACTTTCATGCATCCGTGACCAAAACCCTGTTGTATTTTTTGAGCCCAAGGTAACTCTTTTTCATGAAAGCCTTTCTGAATCTTTTGGATCAAAGTTTGTAAACCAGAATTTAGGAATGCAAATATTTTGACCACGTCTTGGTCTTTAGTGGCTTTACCGTTTAGCAGTGGAAGAAGTTCCTGAGCATGATTTCATGTTGCCTCTATCAGAAGCAGAGGTATGCTCTCTACATTTCCTTTTTTTTTTTTTTCTCTGTTCAATTTTACATCGACTATTCCATATAGCGTTATTTACGCAACATTTGGCAAACCGGAAAATGTGATATTCAGGTGATCCGAGAAGGCAATGACATTACATTGGTTGGTTGGGGAGCACAACTCTCTGTCATGGAACAGGCTTGTATTGATGCTGAAAAAGTAAGTTTCTTAATTTTCTGTTTTGTTTTTATGATTACAGTCTCAAAAAAACCTGGTAAATGAAATTTGAAAATATGATTTATGATAAACAAATGTGGCCTTTTCATGATTTTTTCCTTAAATATTACTATAGAAAGAGATCATGATCCATGAACTATGTGATCAATTCTCCATATTCTGGCCTTTTCTGGTATCTTTATAATGTGACAATTAAACTTAATATGAGTGAGGTTGAGCTATCAATACTCCTGACTTGTTCTAAAACCTTTTGAAATGGTTGAGATTTAGTTAATGTAGGACATGCATAGCTTTTAAAAATTTCAATCTATGAATTCATTAAGTTCCAAATAGGATGGGCCGCTATTTAGAGAGAACGAATGATCTCAATTCTCGACATATTACAGGATGGAATCTCCTGTGAACTGATAGATCTGAGAACTCTACTACCTTGGGACAAGGAAACAGTGGAAGCCTCAGTTAAGAAAACTGGGAGACTTCTTGTAAGTTCTAGTTTCTCTGTTCATAACAAGTTTCCTTAGAACAGTAATTGAGACTAATATAGATTATCATTAATCTCCATTTCTCTCTATAGATCAGCCATGAAGCTCCGGTTACTGGAGGCTTTGGTGCTGAAATATCCGCTTCCATCGTTGAACGCTGCTTCTTACGGGTAAGCATGAATTTCAGTATATTGCCATGATAGTACCAAATTTTTGTTCCTATTTCCTCCTTATATAGTCCAATGCATCTCCATTTCCCTAGACTCTAACATTAGATTGCATCGATATAAATGCTTAGTAGAAATTCCAAAACTTGTTCGAAAGGAAGAATGTTGACGAATGACATTCAATTTGTTGACTACCTAGCATTGCTTATCAAATCTAGTTCTAAAATCTGATATTATTGTGTAGTTCAAATAATCCCATATTAGGACGTAGCTACAATAGTTAATCACTATTAGCAAAACAGAAATATCGAAAAGAAAATCCTCTCCCACAGCCCACAGGTCTGTTTTCAACACCCCTCCCCAGTCCATAGGGCAAGAAATTTTTCCTCTCACTAGACCGTTCCAAGATTAGTATCAAAGCTTAAAATTTGGTTTGAAATATTGAAGGTAAAAAAAAAACCAAAATTATCCTATATGGTCTGTCTACCATAATTATTTGCAGGTTTATAGTAATTTGAAGAATGTCATAAATTTGGTCCTACTTTCGTCTTTAGTTAGAGGCAGAGCGAAGAAGGAAGGATAGAGGCAATTGCACCCTCGTCCTCTGTCATGATTCAAAAAGCATACATAAAATATTTAAGATCTCTGCAATTATCTATAGTTCACCTCCCACTTTTAGAACTAAAAATTGATATATATAAAAGTTAGAACGTAAAATTTTGCCCCTACTGAGAAAATTTATGGCTTCACCTCTTGTAGTCTTTTTTCCTTTTAAAAAAGGCCATCGAAGCGTGATCTTTTAAGTTACGTTTGGTAACGTTTCCATTTCTTATTTATGTTTCTTTATGCATGACCCCTACTGTCCTACATTCATGAATTCAAGTATTAAATTTTTTTTATTTATGTTTTTGAAAAAATTGGAAATGGAAACACAAAAGAAGAACTGTTTTGGTTGTTTCCAAAATTTCCATACAATTTTGAGAATGTTCTAAAAAATGGAGAAACAAGAAACATAGTTAGTTATAAAATGCTTAGATTTATTAAGAAAATTGGAAACGAGAAACAAGAAGTAAGAATGTTATCAAACGAGCCCTTAAGGCTTAATTATCTGTCTCATCTTTTGTTGCTACTCACATGACAGATAAAAATCTAAACTCTTAAAACAAGAACATTAGCTAGTAGAACCCGTACTAGTTTTGAATTTTACAAGTCTTTTATGTTATATTCTTGTGATCTACAAACACAAAAATGAGTCTCTCTGTGTTGTGTTGCAGTTGGAGGCACCTATTTCCAGAGTCTGTGGATTGGACACTCCCTTCCCTCTTGTGTTTGAACCTTTATACATGCCTACCAAGAACAAAGTAAGAGAATATTTTCCATTTTGTTGTGAAACATTTGGCAAAACTTCATTCTTAACCCCACCACACAACTTCTGTGTCTGGTTTAATACAGATATTGGATGCCATCAAATCAACTGTAAAATATTAGATCATAATCAAAGATGAACGCAAACTTGGCCCCATCCTCACAAAAACTGACAACAAGAGGAAGAGGAATGTTGTTGATTCCAGCAGAGTCCCTCATTGAACTGTAAATGATGATTGTCCCATTGTTGCAGACCCTAAAGGGATGCCCTTCACTTCACTGTTAGACAACAATGGATTTTAAATTGTGGATGTATGGATATTGAGATGATTGGGAATAAAAATTATTATATTGTATACATATTTTGTGCATTATCTTTAAGAA

mRNA sequence

ACGTGATCAAGGTTTCATGATATTTTATTTCAATCCGGAAGAACATTCATACAATTTTTCAAACTTAATATATAATTTGATAAAGTTCATCTTTTTATTTGATAAAAATAAACTCTATCTTCAAAGTCTTTTCCAACTTTGTCTCTTTGCATAATTAAGAATTCCCATTACAGAGACGCCATTTGTGTGAGAGAGAGAATTTAATGACTATCAGCAGATACATTGGATAATCTCAATGACTTTTCTGAGTTTTCCGCATTGGATTTTATCCGTTTGAGGCGTTTCATCCGGCAGTTACGCAAAATCGATCATCAAATTGCTGAGAAGGGAAGCTCTGTGTTAATTATCTCTTCCAAATGGTAACTATTTTGAGGAGATCGCAGACAGTGTTGAACTCCATTTGCAGGAGGAAATTTTCTTCTTCCTCTGATGGCGATCTTACGAAACCGAGCGGAAAATCCATGAACCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCGCTCGAAACTGATCCTCGTTCTTATGTATTTGGAGAAGATGTGGGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTGGCAGAACGATACGGGAAGGACCGAGTTTTCAATACCCCTCTTTGCGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTGGCAGCAATGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGATTGTTAATGAAGCTGCAAAGTTCAGATACCGTAGTGGAAATCAGTTCAATTGTGGTGGTTTAACTATTAGAGCACCATATGGAGCTGTTGGTCATGGTGGTCACTATCACTCACAATCACCAGAAGCTTTCTTTTGTCATGTTCCTGGCATCAAAGTTGTCATCCCGCGAAGCCCGTACCAGGCAAAAGGATTGTTACTTTCATGCATCCGTGACCAAAACCCTGTTGTATTTTTTGAGCCCAAGTGGCTTTACCGTTTAGCAGTGGAAGAAGTTCCTGAGCATGATTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAATGACATTACATTGGTTGGTTGGGGAGCACAACTCTCTGTCATGGAACAGGCTTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATAGATCTGAGAACTCTACTACCTTGGGACAAGGAAACAGTGGAAGCCTCAGTTAAGAAAACTGGGAGACTTCTTATCAGCCATGAAGCTCCGGTTACTGGAGGCTTTGGTGCTGAAATATCCGCTTCCATCGTTGAACGCTGCTTCTTACGGTTGGAGGCACCTATTTCCAGAGTCTGTGGATTGGACACTCCCTTCCCTCTTGTGTTTGAACCTTTATACATGCCTACCAAGAACAAAATATTGGATGCCATCAAATCAACTGTAAAATATTAGATCATAATCAAAGATGAACGCAAACTTGGCCCCATCCTCACAAAAACTGACAACAAGAGGAAGAGGAATGTTGTTGATTCCAGCAGAGTCCCTCATTGAACTGTAAATGATGATTGTCCCATTGTTGCAGACCCTAAAGGGATGCCCTTCACTTCACTGTTAGACAACAATGGATTTTAAATTGTGGATGTATGGATATTGAGATGATTGGGAATAAAAATTATTATATTGTATACATATTTTGTGCATTATCTTTAAGAA

Coding sequence (CDS)

ATGGTAACTATTTTGAGGAGATCGCAGACAGTGTTGAACTCCATTTGCAGGAGGAAATTTTCTTCTTCCTCTGATGGCGATCTTACGAAACCGAGCGGAAAATCCATGAACCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCGCTCGAAACTGATCCTCGTTCTTATGTATTTGGAGAAGATGTGGGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTGGCAGAACGATACGGGAAGGACCGAGTTTTCAATACCCCTCTTTGCGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTGGCAGCAATGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCAGATTACATTTATCCAGCTTTTGATCAGATTGTTAATGAAGCTGCAAAGTTCAGATACCGTAGTGGAAATCAGTTCAATTGTGGTGGTTTAACTATTAGAGCACCATATGGAGCTGTTGGTCATGGTGGTCACTATCACTCACAATCACCAGAAGCTTTCTTTTGTCATGTTCCTGGCATCAAAGTTGTCATCCCGCGAAGCCCGTACCAGGCAAAAGGATTGTTACTTTCATGCATCCGTGACCAAAACCCTGTTGTATTTTTTGAGCCCAAGTGGCTTTACCGTTTAGCAGTGGAAGAAGTTCCTGAGCATGATTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAATGACATTACATTGGTTGGTTGGGGAGCACAACTCTCTGTCATGGAACAGGCTTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATAGATCTGAGAACTCTACTACCTTGGGACAAGGAAACAGTGGAAGCCTCAGTTAAGAAAACTGGGAGACTTCTTATCAGCCATGAAGCTCCGGTTACTGGAGGCTTTGGTGCTGAAATATCCGCTTCCATCGTTGAACGCTGCTTCTTACGGTTGGAGGCACCTATTTCCAGAGTCTGTGGATTGGACACTCCCTTCCCTCTTGTGTTTGAACCTTTATACATGCCTACCAAGAACAAAATATTGGATGCCATCAAATCAACTGTAAAATATTAG

Protein sequence

MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY
Homology
BLAST of MC06g0866 vs. ExPASy Swiss-Prot
Match: Q9SAV3 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCDH BETA1 PE=2 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 6.3e-183
Identity = 309/349 (88.54%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 11  VLNSICRR-KFSSSSDG--DLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           +L   CR+  F S S G   ++  +GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLAER+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAK 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AK
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 GLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLS 250
           GLLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREGNDITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 357
           +I+ERCFL+LEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIKSTV Y
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

BLAST of MC06g0866 vs. ExPASy Swiss-Prot
Match: Q9LDY2 (2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIN4 PE=1 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 1.8e-177
Identity = 294/325 (90.46%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 32  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCE 91
           SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAER+GK RVFNTPLCE
Sbjct: 34  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERFGKSRVFNTPLCE 93

Query: 92  QGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 151
           QGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY
Sbjct: 94  QGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 153

Query: 152 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRL 211
           GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AKGLLLS IRD NPVVFFEPKWLYR 
Sbjct: 154 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQ 213

Query: 212 AVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTL 271
           AVE+VPE D+M+PLSEAEV+REG+DITLVGWGAQL++MEQAC+DAE +GISCELIDL+TL
Sbjct: 214 AVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTL 273

Query: 272 LPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPF 331
           +PWDKE VE SV+KTGRLLISHEAPVTGGFGAEI+A+IVERCFLRLEAP+SRVCGLDTPF
Sbjct: 274 IPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPF 333

Query: 332 PLVFEPLYMPTKNKILDAIKSTVKY 357
           PLVFEP YMPTKNKILDAI+STV Y
Sbjct: 334 PLVFEPFYMPTKNKILDAIRSTVNY 358

BLAST of MC06g0866 vs. ExPASy Swiss-Prot
Match: P21839 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus OX=9913 GN=BCKDHB PE=1 SV=2)

HSP 1 Score: 476.9 bits (1226), Expect = 2.0e-133
Identity = 222/324 (68.52%), Postives = 265/324 (81.79%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ A+  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 69  QKMNLFQAVTSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 128

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIR+P+G 
Sbjct: 129 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRSPWGC 188

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVV+PRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 189 VGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 248

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT+L
Sbjct: 249 EQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVAAMAQEKLGVSCEVIDLRTIL 308

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLL+SHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 309 PWDVDTVCKSVIKTGRLLVSHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 368

Query: 334 LVFEPLYMPTKNKILDAIKSTVKY 357
            +FEP Y+P K K  DA++  + Y
Sbjct: 369 HIFEPFYIPDKWKCYDALRKMINY 392

BLAST of MC06g0866 vs. ExPASy Swiss-Prot
Match: Q6P3A8 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10090 GN=Bckdhb PE=1 SV=2)

HSP 1 Score: 476.9 bits (1226), Expect = 2.0e-133
Identity = 224/324 (69.14%), Postives = 265/324 (81.79%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVVIPRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 246

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 247 EQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIV 306

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 307 PWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 366

Query: 334 LVFEPLYMPTKNKILDAIKSTVKY 357
            +FEP Y+P K K  DA++  + Y
Sbjct: 367 HIFEPFYIPDKWKCYDALRKMINY 390

BLAST of MC06g0866 vs. ExPASy Swiss-Prot
Match: P35738 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus OX=10116 GN=Bckdhb PE=1 SV=3)

HSP 1 Score: 476.9 bits (1226), Expect = 2.0e-133
Identity = 224/324 (69.14%), Postives = 265/324 (81.79%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL ++YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV 213
           VGHG  YHSQSPEAFF H PGIKVVIPRSP+QAKGLLLSCI D+NP +FFEPK LYR AV
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAV 246

Query: 214 EEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRTLL 273
           E+VP   + +PLS+AEVI+EG+D+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT++
Sbjct: 247 EQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTIV 306

Query: 274 PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFP 333
           PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAPISRVCG DTPFP
Sbjct: 307 PWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFP 366

Query: 334 LVFEPLYMPTKNKILDAIKSTVKY 357
            +FEP Y+P K K  DA++  + Y
Sbjct: 367 HIFEPFYIPDKWKCYDALRKMINY 390

BLAST of MC06g0866 vs. NCBI nr
Match: XP_022147692.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Momordica charantia])

HSP 1 Score: 727 bits (1877), Expect = 8.39e-265
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNKILDAIKSTVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKILDAIKSTVKY 356

BLAST of MC06g0866 vs. NCBI nr
Match: XP_023515905.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704 bits (1816), Expect = 1.67e-255
Identity = 340/356 (95.51%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. NCBI nr
Match: XP_022960762.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata] >KAG6590071.1 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702 bits (1812), Expect = 6.80e-255
Identity = 339/356 (95.22%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. NCBI nr
Match: XP_008450996.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis melo])

HSP 1 Score: 695 bits (1794), Expect = 3.77e-252
Identity = 333/356 (93.54%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT  +GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQAC+DAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. NCBI nr
Match: XP_004144063.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis sativus] >KAE8653468.1 hypothetical protein Csa_007124 [Cucumis sativus])

HSP 1 Score: 695 bits (1793), Expect = 5.36e-252
Identity = 334/356 (93.82%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT  +GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPVVFFEPKWLYRLAVEEVPE DFMLPLS+A+VIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVVFFEPKWLYRLAVEEVPEDDFMLPLSQADVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. ExPASy TrEMBL
Match: A0A6J1D329 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016561 PE=4 SV=1)

HSP 1 Score: 727 bits (1877), Expect = 4.06e-265
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNKILDAIKSTVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKILDAIKSTVKY 356

BLAST of MC06g0866 vs. ExPASy TrEMBL
Match: A0A6J1H8B9 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461465 PE=4 SV=1)

HSP 1 Score: 702 bits (1812), Expect = 3.29e-255
Identity = 339/356 (95.22%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. ExPASy TrEMBL
Match: A0A1S3BQK1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492412 PE=4 SV=1)

HSP 1 Score: 695 bits (1794), Expect = 1.83e-252
Identity = 333/356 (93.54%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           M ++LRRSQTVLNS+CRR+FS+S DG+LT  +GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRD+NPV+FFEPKWLYRLAVEEVPE DFMLPLS+AEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQAC+DAEK+GISCELIDLRTLLPWDKETVEASV+KTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. ExPASy TrEMBL
Match: A0A6J1JGI1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484908 PE=4 SV=1)

HSP 1 Score: 691 bits (1784), Expect = 6.11e-251
Identity = 336/356 (94.38%), Postives = 347/356 (97.47%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +L+SICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL +RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLV 240
           RSPYQAKGLLLSCIRDQNPVVFFEPK LYRLAVEEVPE DFMLPLSEAEVIREG+DITLV
Sbjct: 181 RSPYQAKGLLLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDITLV 240

Query: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGG 300
           GWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVTGG
Sbjct: 241 GWGAQLSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVTGG 300

Query: 301 FGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           FGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 FGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of MC06g0866 vs. ExPASy TrEMBL
Match: A0A660KMJ8 (Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_009543 PE=4 SV=1)

HSP 1 Score: 653 bits (1685), Expect = 7.77e-236
Identity = 312/351 (88.89%), Postives = 334/351 (95.16%), Query Frame = 0

Query: 9   QTVLNSICR---RKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 68
           +T+  SICR   R FS+S D  L +PS KS NLYSAINQALHIALETDPR+YVFGEDVGF
Sbjct: 7   RTIGRSICRVSRRGFSTSCDDKLVQPSSKSTNLYSAINQALHIALETDPRAYVFGEDVGF 66

Query: 69  GGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 128
           GGVFRCTTGLA+R+GK RVFNTPLCEQGI+GFGIGLAAMGNRA+AEIQFADYIYPAFDQI
Sbjct: 67  GGVFRCTTGLADRFGKSRVFNTPLCEQGIIGFGIGLAAMGNRAVAEIQFADYIYPAFDQI 126

Query: 129 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQ 188
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP Q
Sbjct: 127 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPQQ 186

Query: 189 AKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQ 248
           AKGLLLSCIRD NPVVFFEPKWLYRLAVEEVPE+D+MLPLSEAEVIREG+DITLVGWGAQ
Sbjct: 187 AKGLLLSCIRDPNPVVFFEPKWLYRLAVEEVPEYDYMLPLSEAEVIREGSDITLVGWGAQ 246

Query: 249 LSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 308
           LSVMEQAC+DAEK+GISCELIDL+TL+PWDKET+E+SV+KTGRLLISHEAPVTGGFGAEI
Sbjct: 247 LSVMEQACLDAEKEGISCELIDLKTLIPWDKETIESSVRKTGRLLISHEAPVTGGFGAEI 306

Query: 309 SASIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 356
           SASIVERCFLRLEAP++RVCGLDTPFPLVFEP YMPTKNKILDAIKST+ Y
Sbjct: 307 SASIVERCFLRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTINY 357

BLAST of MC06g0866 vs. TAIR 10
Match: AT1G55510.1 (branched-chain alpha-keto acid decarboxylase E1 beta subunit )

HSP 1 Score: 641.3 bits (1653), Expect = 4.5e-184
Identity = 309/349 (88.54%), Postives = 329/349 (94.27%), Query Frame = 0

Query: 11  VLNSICRR-KFSSSSDG--DLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           +L   CR+  F S S G   ++  +GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLAER+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAK 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AK
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 GLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLS 250
           GLLLSCIRD NPVVFFEPKWLYR AVEEVPEHD+M+PLSEAEVIREGNDITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPISRVCGLDTPFPLVFEPLYMPTKNKILDAIKSTVKY 357
           +I+ERCFL+LEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIKSTV Y
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

BLAST of MC06g0866 vs. TAIR 10
Match: AT3G13450.1 (Transketolase family protein )

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-178
Identity = 294/325 (90.46%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 32  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERYGKDRVFNTPLCE 91
           SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAER+GK RVFNTPLCE
Sbjct: 34  SGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERFGKSRVFNTPLCE 93

Query: 92  QGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 151
           QGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY
Sbjct: 94  QGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPY 153

Query: 152 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRL 211
           GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSP +AKGLLLS IRD NPVVFFEPKWLYR 
Sbjct: 154 GAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQ 213

Query: 212 AVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTL 271
           AVE+VPE D+M+PLSEAEV+REG+DITLVGWGAQL++MEQAC+DAE +GISCELIDL+TL
Sbjct: 214 AVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTL 273

Query: 272 LPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPISRVCGLDTPF 331
           +PWDKE VE SV+KTGRLLISHEAPVTGGFGAEI+A+IVERCFLRLEAP+SRVCGLDTPF
Sbjct: 274 IPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPF 333

Query: 332 PLVFEPLYMPTKNKILDAIKSTVKY 357
           PLVFEP YMPTKNKILDAI+STV Y
Sbjct: 334 PLVFEPFYMPTKNKILDAIRSTVNY 358

BLAST of MC06g0866 vs. TAIR 10
Match: AT5G50850.1 (Transketolase family protein )

HSP 1 Score: 229.6 bits (584), Expect = 4.0e-60
Identity = 133/357 (37.25%), Postives = 193/357 (54.06%), Query Frame = 0

Query: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60
           M+ ILR+      S  RR   +           K M +  A+N A+   +  DP+ +V G
Sbjct: 1   MLGILRQRAIDGASTLRRTRFALVSARSYAAGAKEMTVRDALNSAIDEEMSADPKVFVMG 60

Query: 61  EDVG-FGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIY 120
           E+VG + G ++ T GL E+YG +RV++TP+ E G  G G+G A  G + + E    ++  
Sbjct: 61  EEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEFMTFNFSM 120

Query: 121 PAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVI 180
            A D I+N AAK  Y S  Q N   +  R P GA    G  HSQ   A++  VPG+KV+ 
Sbjct: 121 QAIDHIINSAAKSNYMSAGQINV-PIVFRGPNGAAAGVGAQHSQCYAAWYASVPGLKVLA 180

Query: 181 PRSPYQAKGLLLSCIRDQNPVVFFEPKWLYRLAV---EEVPEHDFMLPLSEAEVIREGND 240
           P S   A+GLL + IRD +PVVF E + LY  +    EE  +  F LP+ +A++ REG D
Sbjct: 181 PYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKIEREGKD 240

Query: 241 ITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAP 300
           +T+V +   +    +A     ++GIS E+I+LR++ P D+ T+ ASV+KT RL+   E  
Sbjct: 241 VTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGF 300

Query: 301 VTGGFGAEISASIVERCFLRLEAPISRVCGLDTPFPLV--FEPLYMPTKNKILDAIK 352
              G  AEI AS+VE  F  L+AP+ R+ G D P P     E L +P    I+ A K
Sbjct: 301 PQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRASK 356

BLAST of MC06g0866 vs. TAIR 10
Match: AT2G34590.1 (Transketolase family protein )

HSP 1 Score: 219.2 bits (557), Expect = 5.5e-57
Identity = 123/330 (37.27%), Postives = 184/330 (55.76%), Query Frame = 0

Query: 4   ILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDV 63
           + RRS+ ++ +    K  +++    +KP G  + L+ A+ + L   ++ DP   V GEDV
Sbjct: 55  VARRSEPLIPNAVTTKADTAASSTSSKP-GHELLLFEALQEGLEEEMDRDPHVCVMGEDV 114

Query: 64  G-FGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAF 123
           G +GG ++ T GLA+++G  RV +TP+CE    G GIG A  G R + E     ++  AF
Sbjct: 115 GHYGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAF 174

Query: 124 DQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRS 183
           +QI N      Y SG QF    + IR P G     G  HSQ  E++F  +PGI++V   +
Sbjct: 175 NQISNNCGMLHYTSGGQFTI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACST 234

Query: 184 PYQAKGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGW 243
           PY AKGL+ + IR +NPV+ FE   LY L  E +P+ +++  L EAE++R G  IT++ +
Sbjct: 235 PYNAKGLMKAAIRSENPVILFEHVLLYNLK-ESIPDEEYICNLEEAEMVRPGEHITILTY 294

Query: 244 GAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFG 303
                 + QA       G   E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG G
Sbjct: 295 SRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIG 354

Query: 304 AEISASIVERCFLRLEAPISRVCGLDTPFP 333
           A ++A+I E     L+AP+  +   D P P
Sbjct: 355 ASLTAAINENFHDYLDAPVMCLSSQDVPTP 381

BLAST of MC06g0866 vs. TAIR 10
Match: AT1G30120.1 (pyruvate dehydrogenase E1 beta )

HSP 1 Score: 218.8 bits (556), Expect = 7.1e-57
Identity = 119/312 (38.14%), Postives = 176/312 (56.41%), Query Frame = 0

Query: 22  SSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFGEDVG-FGGVFRCTTGLAERYG 81
           ++ + D +  +G  + L+ A+ + L   ++ DP   V GEDVG +GG ++ T GLA+++G
Sbjct: 72  ATKEADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVTKGLADKFG 131

Query: 82  KDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQF 141
             RV +TP+CE    G GIG A  G R + E     ++  AF+QI N      Y SG QF
Sbjct: 132 DLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGMLHYTSGGQF 191

Query: 142 NCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPYQAKGLLLSCIRDQNPV 201
               + IR P G     G  HSQ  E++F  +PGI++V   +PY AKGL+ + IR +NPV
Sbjct: 192 TI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPV 251

Query: 202 VFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDITLVGWGAQLSVMEQACIDAEKDG 261
           + FE   LY L  E++P+ D++  L EAE++R G  IT++ +      + QA       G
Sbjct: 252 ILFEHVLLYNLK-EKIPDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKG 311

Query: 262 ISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAP 321
              E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA ++A+I E     L+AP
Sbjct: 312 YDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAP 371

Query: 322 ISRVCGLDTPFP 333
           +  +   D P P
Sbjct: 372 VMCLSSQDVPTP 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAV36.3e-18388.542-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thal... [more]
Q9LDY21.8e-17790.462-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thal... [more]
P218392.0e-13368.522-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Bos taurus OX=9913... [more]
Q6P3A82.0e-13369.142-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10... [more]
P357382.0e-13369.142-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus ... [more]
Match NameE-valueIdentityDescription
XP_022147692.18.39e-26599.722-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Momordica charanti... [more]
XP_023515905.11.67e-25595.512-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbi... [more]
XP_022960762.16.80e-25595.222-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata... [more]
XP_008450996.13.77e-25293.54PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis... [more]
XP_004144063.15.36e-25293.822-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis sativus] >... [more]
Match NameE-valueIdentityDescription
A0A6J1D3294.06e-26599.722-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charan... [more]
A0A6J1H8B93.29e-25595.222-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moscha... [more]
A0A1S3BQK11.83e-25293.542-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=... [more]
A0A6J1JGI16.11e-25194.382-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima... [more]
A0A660KMJ87.77e-23688.89Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_0... [more]
Match NameE-valueIdentityDescription
AT1G55510.14.5e-18488.54branched-chain alpha-keto acid decarboxylase E1 beta subunit [more]
AT3G13450.11.3e-17890.46Transketolase family protein [more]
AT5G50850.14.0e-6037.25Transketolase family protein [more]
AT2G34590.15.5e-5737.27Transketolase family protein [more]
AT1G30120.17.1e-5738.14pyruvate dehydrogenase E1 beta [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005475Transketolase-like, pyrimidine-binding domainSMARTSM00861Transket_pyr_3coord: 36..211
e-value: 3.9E-66
score: 235.7
IPR005475Transketolase-like, pyrimidine-binding domainPFAMPF02779Transket_pyrcoord: 36..210
e-value: 7.1E-44
score: 149.6
NoneNo IPR availableGENE3D3.40.50.970coord: 32..224
e-value: 4.8E-95
score: 318.1
NoneNo IPR availablePANTHERPTHR429802-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATEDcoord: 21..356
NoneNo IPR availableCDDcd07036TPP_PYR_E1-PDHc-beta_likecoord: 41..206
e-value: 9.08931E-100
score: 290.149
IPR033248Transketolase, C-terminal domainPFAMPF02780Transketolase_Ccoord: 227..346
e-value: 5.1E-36
score: 123.3
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IIGENE3D3.40.50.920coord: 225..355
e-value: 3.8E-48
score: 164.8
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IISUPERFAMILY52922TK C-terminal domain-likecoord: 220..356
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 33..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0866.1MC06g0866.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009083 branched-chain amino acid catabolic process
biological_process GO:0007584 response to nutrient
cellular_component GO:0005947 mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function GO:0003824 catalytic activity