MC06g0810 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0810 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 5.4e-200
Identity = 381/716 (53.21%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 44  RSIRCTIPQSGVG----SSPSPEIARM--SPLKPPKIPVESTV---RRPSFARSSFSKPK 103
           RS     P+S  G     SPSPEI+++  SP KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGS 163
           SR V+P CP   ++ E++ + +  +G  ++ +        S R     AP+TP   ++  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS---PNNKSNRSVGSPAPVTPSKVVV-- 147

Query: 164 IGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIW 223
              E+++DEE+YK  +L  + RS  K+    LIE   F+ I    +ASLTI+ L+    W
Sbjct: 148 ---EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHTFW 207

Query: 224 GLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLA 283
           GL +W+WCVLV+VIF G L + WF+  +VFLIE NFLLRRKVLYFV+GL+KSV +FIWL 
Sbjct: 208 GLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLC 267

Query: 284 LVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRF 343
           L+L+AW LLF+H  KRS  A  +L  +TR L + L GA  WL+KTLL+KILAA+F    F
Sbjct: 268 LILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNF 327

Query: 344 FDRIQESIFHQYILRTLSGPPLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDV 403
           FDRIQ+S+FHQY+L+TLSG PL+E AERVGR  STG LSF  + K+   G   +++VID+
Sbjct: 328 FDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVIDM 387

Query: 404 DKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFNEEESEQKDKEINSEWEARAA 463
            K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA AA
Sbjct: 388 GKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAA 447

Query: 464 AYQIFRNVAKPGSKYIDEEDLFRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYV 523
           AY +FRNVA+P   YI+EEDL RFM KEEV+ V PLF+GA ETG+I RK    W+V VY 
Sbjct: 448 AYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYT 507

Query: 524 ERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFM 583
            R++LAHSLNDTKTA+++LNKL +A++++V +++WLLL+ + TT++L+F S+QL+ + F+
Sbjct: 508 SRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFI 567

Query: 584 FGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSV 643
            G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+V
Sbjct: 568 IGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAV 627

Query: 644 LATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKE 703
           LATKPISN++RSP M +++EFS+ FST +  I  LK RI  YLE  PQ W P HSVVVKE
Sbjct: 628 LATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKE 687

Query: 704 IENVNKMKMGLYVNHTINFQNYGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           IEN+NK+KM LY +HTI FQ   +++ RR++L L +K++LE+L I Y LLPQ++ L
Sbjct: 688 IENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of MC06g0810 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 1.3e-177
Identity = 364/755 (48.21%), Postives = 499/755 (66.09%), Query Frame = 0

Query: 8   EQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPSPEIARMS 67
           E+VV+ + D E + D     +   +AS PD +      I  + P   + S P+PEI + S
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-PDSD----AGIEKSKPVPPI-SIPTPEIYKFS 69

Query: 68  PL--KPPKIP-VESTVRRPSFARSSFSKPKSRLVEPPC---------PGGANLAEDKAQA 127
               KPPKIP  E  VRR S +RS +SKPKSR  E             GG +L E     
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDD-EEVYKTAELKVK 187
               GS    SP       S R    +A        +  +  EE D+ EE+YK  +L   
Sbjct: 130 SFARGSFDRASP----NNKSNRSVASAA--------LSKVAEEEPDENEEIYKKVKLHRV 189

Query: 188 ERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLF 247
           +RSG  +K    +E V F+ I G  I SLTI  +    IWGL  W+WCVLV+V   G L 
Sbjct: 190 KRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLV 249

Query: 248 SQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKA 307
           + WF++ +VF+IE+N+LLR+KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR++K 
Sbjct: 250 TNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKT 309

Query: 308 NDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 367
              L+++T  + + L G+ L+L+KT  +K+LA+ F    FF+RIQES+FHQY+L+TLSGP
Sbjct: 310 KRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGP 369

Query: 368 PLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 427
           PLIE AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI
Sbjct: 370 PLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLI 429

Query: 428 NVIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 487
             + +SG+STIS+T++  N ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL
Sbjct: 430 EAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDL 489

Query: 488 FRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 547
            RFM KEEV+ VLPL E A +TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+K
Sbjct: 490 LRFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDK 549

Query: 548 LASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 607
           L + ++ ++  IVW++L+ + +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP+
Sbjct: 550 LITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPY 609

Query: 608 DVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 667
           DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D ++F
Sbjct: 610 DVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDF 669

Query: 668 SVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQN 727
            + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ + + V HTINFQ 
Sbjct: 670 GIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQV 729

Query: 728 YGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           Y +KS RR+ L++ +K+ILE+L I Y LLPQ+V L
Sbjct: 730 YVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of MC06g0810 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.1e-149
Identity = 306/712 (42.98%), Postives = 442/712 (62.08%), Query Frame = 0

Query: 92  KPKSRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPL 151
           K +SRL++PP P   ++   +          ++    K                 P TP 
Sbjct: 185 KTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTK-----------------PGTPN 244

Query: 152 IGSIGSEE-EDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQT 211
            G  GS++ E++E+ +   +L    R   K+ + ++IEW+  + I    I SL I  L+ 
Sbjct: 245 QG--GSKDLEEEEDPFSEEDLPEGLRK-EKICVWVIIEWIFLILIIASLICSLVIPYLRG 304

Query: 212 TEIWGLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIF 271
             +W L LW+W V+VLV+ CGRL S W +   V+ +E NFL R+KVLYFVYG+RK V   
Sbjct: 305 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 364

Query: 272 IWLALVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQ 331
           +WL LVL+AW  LFD   +R  ++  +L YVT+ L   L    +WL+KTLLVK+LA+SF 
Sbjct: 365 LWLGLVLIAWHFLFDKKVEREMRST-VLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFH 424

Query: 332 CTRFFDRIQESIFHQYILRTLSGPPLIEM---AERVGRTASTGQLSFRHLK--------- 391
            + +FDRIQES+F QY++ TLSGPP IE+    E+V     T ++  R L          
Sbjct: 425 MSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSS 484

Query: 392 ------------------------KQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 451
                                    +S    EG EE I +D L++M  + +SAW M+ L+
Sbjct: 485 PPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLM 544

Query: 452 NVIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 511
           NVI+   LST+   I++   +E + K  +I SE+EA+ AA +IF+NVA+PGS+YI  ED 
Sbjct: 545 NVIKKGTLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDF 604

Query: 512 FRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 571
            RF+S++E E  + LFEGA E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L++
Sbjct: 605 MRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHR 664

Query: 572 LASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 631
           +   +V IVI+I+WLL++G+ TT+ L+ ISSQLLLVVF+FGN+ +T+FEA+IFVFVMHPF
Sbjct: 665 IVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPF 724

Query: 632 DVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 691
           DVGDRC +DGVQM+VEEMNILTT+FLRFDN+KI YPNS+L TKPI+N+YRSP+M D+IEF
Sbjct: 725 DVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEF 784

Query: 692 SVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQN 751
            V  +T  E   AL+ RI SY+++K   W P   +V +++  +N +K+ ++  H +N QN
Sbjct: 785 FVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQN 844

Query: 752 YGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPM----VPPS 763
            G++  RR  L+ E+ ++  EL I+Y L P  + + S+ +A P+    +PPS
Sbjct: 845 MGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPS 874

BLAST of MC06g0810 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.0e-149
Identity = 310/715 (43.36%), Postives = 451/715 (63.08%), Query Frame = 0

Query: 81  RRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDAL 140
           ++P  +R   +K KSRL +PP P   + A DK + KS   S                   
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTP--THPAIDKTEMKSGRRS----------------GIF 244

Query: 141 KSAPI--TPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGC 200
           KS  +  +PK    G  G EEE++E+ +   +L  +E    KL   + +EW++ + I   
Sbjct: 245 KSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLIVTS 304

Query: 201 FIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLY 260
            + SLTIH LQ    W L LW+W V VLV+ CGRL S W +  +VFL+E+NF  R++VLY
Sbjct: 305 LVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLY 364

Query: 261 FVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLK 320
           FVYG+RKSV   +WL LVLLAW  LFD   +R  ++   L YVTR L   L    +WL+K
Sbjct: 365 FVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRST-ALRYVTRVLVCLLVALIIWLVK 424

Query: 321 TLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIE---MAERVGRTASTGQ---- 380
           T+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPL+E   M E   + A   +    
Sbjct: 425 TILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEK 484

Query: 381 --------------LSFRHLKKQSD----GGNEGKE-EVIDVDKLKKMKQEKISAWTMRG 440
                          SF  + K       G   G++ E I +D+LK+M  + +SAW M+ 
Sbjct: 485 LAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKR 544

Query: 441 LINVIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEE 500
           L+N+I    +ST+   +++  +E+ +     I SE+EA+ AA +IF NV +PGS+YI  E
Sbjct: 545 LMNIILKGAISTLDQNMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYLE 604

Query: 501 DLFRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEEL 560
           D  RF+ +EE E  + LFEGA E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ L
Sbjct: 605 DFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRL 664

Query: 561 NKLASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMH 620
           +++ + V+ I+III+WLL++G+ TT+ L+ +SSQLLLV F+FGN+ +T+FEAIIF+FVMH
Sbjct: 665 HRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMH 724

Query: 621 PFDVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSI 680
           PFDVGDRC +DGVQ+VVEEMNILTT+FLR+DN+KI YPNSVL TKPI+N+YRSP+M D++
Sbjct: 725 PFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAV 784

Query: 681 EFSVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINF 740
           EF V  +T  E I A+K RI SY+++K  +W P   +V   ++++N +K+ +++ H +N 
Sbjct: 785 EFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNH 844

Query: 741 QNYGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSV-----GSAAPMVPPS 763
           Q+ G++  RR  L+ E+ K   EL I+Y L P  + + S+      +++  +PPS
Sbjct: 845 QDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874

BLAST of MC06g0810 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.3e-145
Identity = 304/700 (43.43%), Postives = 438/700 (62.57%), Query Frame = 0

Query: 92  KPKSRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPL 151
           + +SRL +PP P    L    A  KS                  P+     +    K+P 
Sbjct: 172 RTRSRLSDPPTP---QLPPQTADMKS---------------GRIPKSGQMKSGFFGKSP- 231

Query: 152 IGSIGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTT 211
             + G EEEDD      AE   +E    KL + I++EW++ + I   F+ +L I  L+  
Sbjct: 232 -KTQGEEEEDDP---FAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKK 291

Query: 212 EIWGLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFI 271
           ++W L LW+W  +VLV+ CGRL S W +  +VF IERNFLLR++VLYFVYG+RK+V   +
Sbjct: 292 KLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCL 351

Query: 272 WLALVLLAWGLLFDHSRKRSKKAN-DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQ 331
           WL LVLLAW  LFD   K +K AN   L  VT+     L G  LWL+KTLLVK+LA+SF 
Sbjct: 352 WLGLVLLAWHFLFD--EKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 411

Query: 332 CTRFFDRIQESIFHQYILRTLSGPPLIEM------AERVG-------------------- 391
            + +FDRIQES+F QY++ TLSGPPLIE+       ER+                     
Sbjct: 412 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 471

Query: 392 RTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLST 451
               TG+  F      + GG  G+ + I +D L K+  + +SAW M+ L+N+IR+  L+T
Sbjct: 472 SPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTT 531

Query: 452 ISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEVE 511
           +   ++  +    + K  +I SE+EA+ AA +IF NVAKPGSK+I   D+ RF+  +E  
Sbjct: 532 LDEQLQ--DPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEAL 591

Query: 512 NVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVVLIVI 571
             L LFEGA ET +I + +LKNW+VN + ER++LA +LNDTKTA+  L+K+ + VV I+I
Sbjct: 592 KTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIII 651

Query: 572 IIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDG 631
           +++WL+++G+ +T+ LV +SSQ+++V F+FGN  + VFE+II++FV+HPFDVGDRC +DG
Sbjct: 652 LVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDG 711

Query: 632 VQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFSTSIES 691
           VQMVVEEMNILTT+FLRFDN+K+ YPNS+L TK I N+YRSP+M D IEFS+  +T  E 
Sbjct: 712 VQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEK 771

Query: 692 IGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSSRRSD 751
           I  +K RI SY+E K   W P   +V K++E++N +++ ++  H +N Q+ G+K +RRS 
Sbjct: 772 IILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQ 831

Query: 752 LVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 765
           LV E+ KI  EL I+Y L P ++ + ++ ++  + P S R
Sbjct: 832 LVEEIAKICRELDIEYRLYPLDINVRNLPTSTAL-PVSDR 843

BLAST of MC06g0810 vs. NCBI nr
Match: XP_022147669.1 (mechanosensitive ion channel protein 10-like [Momordica charantia])

HSP 1 Score: 1456 bits (3768), Expect = 0.0
Identity = 764/764 (100.00%), Postives = 764/764 (100.00%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
           PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK 180
           SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK
Sbjct: 121 SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK 180

Query: 181 LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN 240
           LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN
Sbjct: 181 LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN 240

Query: 241 CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY 300
           CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY
Sbjct: 241 CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY 300

Query: 301 VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA 360
           VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA
Sbjct: 301 VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA 360

Query: 361 ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS 420
           ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS
Sbjct: 361 ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS 420

Query: 421 GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 480
           GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK
Sbjct: 421 GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 480

Query: 481 EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV 540
           EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV
Sbjct: 481 EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV 540

Query: 541 LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 600
           LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC
Sbjct: 541 LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 600

Query: 601 VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST 660
           VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST
Sbjct: 601 VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST 660

Query: 661 SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS 720
           SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS
Sbjct: 661 SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS 720

Query: 721 RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR
Sbjct: 721 RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764

BLAST of MC06g0810 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1276 bits (3303), Expect = 0.0
Identity = 666/765 (87.06%), Postives = 713/765 (93.20%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+G EQ+VLRIL+ E  VD +KD TK SV+S PDF+LKETRS RCTIPQS VGSSPS
Sbjct: 1   MADKKGMEQLVLRILEGEEGVDTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTS- 120
            EI+RMSPLKPPKIP E+ +RRPSFARSSFSKPKSRL+E PCP  A+LAE+KA+AKST  
Sbjct: 61  HEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKSTLY 120

Query: 121 GSPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGR 180
            SP  +SPAKITT TSP++ALKSAPITPKTPLIG+ GSEEEDDEEVYKTAELKVKERSG+
Sbjct: 121 SSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGK 180

Query: 181 KLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFI 240
           KLK T+LIEWVAFLC+TGC IASLTI KL T EIWGLGLW+WCVLVLV FCGRLFSQWFI
Sbjct: 181 KLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFSQWFI 240

Query: 241 NCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILN 300
           NCLVFLIERNFLL+RKVLYFVYGLRKSVIIFIWL LVLLAWGLLFD S KRSKK N+ILN
Sbjct: 241 NCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGNEILN 300

Query: 301 YVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEM 360
           Y+TRAL ASL GAGLWL+KTL+VKILAASFQC RFFDRIQESIFHQYILR LSGPP++EM
Sbjct: 301 YITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPPIMEM 360

Query: 361 AERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS 420
           AE VGR ASTGQLSFRHLKK+SDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS
Sbjct: 361 AESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS 420

Query: 421 SGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMS 480
           SGLSTISNTIENF EEE+EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDE+DLFRFMS
Sbjct: 421 SGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMS 480

Query: 481 KEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAV 540
           KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELNKLASA+
Sbjct: 481 KEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKLASAI 540

Query: 541 VLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 600
           VLIVIII WLLLMG LTTQ+LVF+SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR
Sbjct: 541 VLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 600

Query: 601 CVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFS 660
           CVVDGVQMVVEEMNILTTIFLR+DNEKIFYPNSVLATKPISN+YRSPEMSDSIEFSVDFS
Sbjct: 601 CVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEFSVDFS 660

Query: 661 TSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKS 720
           TSIESIGALKARIK+YLESKPQFWRP HSV+VKEIEN+NKMK+ L VNHTINFQNYGDKS
Sbjct: 661 TSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNYGDKS 720

Query: 721 SRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           SRRSDLVLELKKI E+LGIKYHLLPQEV LN VGSAA ++P S R
Sbjct: 721 SRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPSSQR 765

BLAST of MC06g0810 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1270 bits (3286), Expect = 0.0
Identity = 664/769 (86.35%), Postives = 708/769 (92.07%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+GTEQVVLRILD E  VD     T  SVAS PDFE KETRS+RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
            EI+RMS LKPPKIPVES VRRPSFARSSFSKPKSRL+EPPCP GA+ AE+ AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SPYNNSP-----AKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180
           SP+++SP     AKI TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240
           +S +KLK  +LIEW+AFLCIT C I+SLTI KL T EIWGLGLW+WCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+FIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVGR AS GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIIIVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN V S APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of MC06g0810 vs. NCBI nr
Match: KAG7023687.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1262 bits (3265), Expect = 0.0
Identity = 658/755 (87.15%), Postives = 702/755 (92.98%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+GTEQVVLRILD E  VD   D T  SVAS PDFE KETRS+RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
            EI+RMS LKPPKIPV S VRRPSFARSSFSKPKSRL+EPPCP GA+LAE+ AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVGSAVRRPSFARSSFSKPKSRLIEPPCPDGASLAEENAQAKSTSG 120

Query: 121 SPYNNSP-----AKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180
           SP+++SP     A I TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240
           +S +KLK  +LIEW+AFLCIT C I+SLTI KL T EIWGLGLW+WCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+FIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVG  AS+GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIIIVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLN 750
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLN 755

BLAST of MC06g0810 vs. NCBI nr
Match: XP_023515637.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1260 bits (3261), Expect = 0.0
Identity = 660/769 (85.83%), Postives = 707/769 (91.94%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+GTEQVVLRILD E  VD   D T  SVAS PDFE KETRS+RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
            EI+RMS LKPPKIPV S VRRPSFARSSFSKPKSRL+EPPCP GA+L E+ AQAKS SG
Sbjct: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120

Query: 121 SPYNNSP-----AKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180
           SP+++SP     A I TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240
           +S +KLK  +LIEW+AFLCIT C I+SLTI KL T EIWGL LW+WCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+ IWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVGR AS+GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVI+IVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN V + APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSR 768

BLAST of MC06g0810 vs. ExPASy TrEMBL
Match: A0A6J1D1P7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111016536 PE=3 SV=1)

HSP 1 Score: 1456 bits (3768), Expect = 0.0
Identity = 764/764 (100.00%), Postives = 764/764 (100.00%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
           PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK 180
           SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK
Sbjct: 121 SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK 180

Query: 181 LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN 240
           LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN
Sbjct: 181 LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN 240

Query: 241 CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY 300
           CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY
Sbjct: 241 CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY 300

Query: 301 VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA 360
           VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA
Sbjct: 301 VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA 360

Query: 361 ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS 420
           ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS
Sbjct: 361 ERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSS 420

Query: 421 GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 480
           GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK
Sbjct: 421 GLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSK 480

Query: 481 EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV 540
           EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV
Sbjct: 481 EEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVV 540

Query: 541 LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 600
           LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC
Sbjct: 541 LIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRC 600

Query: 601 VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST 660
           VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST
Sbjct: 601 VVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDFST 660

Query: 661 SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS 720
           SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS
Sbjct: 661 SIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDKSS 720

Query: 721 RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR
Sbjct: 721 RRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764

BLAST of MC06g0810 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1270 bits (3286), Expect = 0.0
Identity = 664/769 (86.35%), Postives = 708/769 (92.07%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+GTEQVVLRILD E  VD     T  SVAS PDFE KETRS+RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
            EI+RMS LKPPKIPVES VRRPSFARSSFSKPKSRL+EPPCP GA+ AE+ AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SPYNNSP-----AKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180
           SP+++SP     AKI TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240
           +S +KLK  +LIEW+AFLCIT C I+SLTI KL T EIWGLGLW+WCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+FIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVGR AS GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIIIVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN V S APMV  + R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of MC06g0810 vs. ExPASy TrEMBL
Match: A0A6J1H896 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111461449 PE=3 SV=1)

HSP 1 Score: 1257 bits (3253), Expect = 0.0
Identity = 658/764 (86.13%), Postives = 704/764 (92.15%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60
           MADK+G EQVVLRILD E  VD     T  SVAS PDFE KETRS+RC I QS V SSPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120
            EI+RMS  KPPKIPVES VRRPSFARSSFSKPKSRL+EPP P GA+LAE+ AQAKS SG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SPYNNSP-----AKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180
           SP+++SP     AKI TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240
           +S +KLK  ++IEW+AFLCIT C I+SLTI KL T EIWGLGLW+WCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+FIWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           L+EMAERVG  AS+GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIIIVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMV 759
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN + S APMV
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVAPMV 764

BLAST of MC06g0810 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1237 bits (3200), Expect = 0.0
Identity = 650/759 (85.64%), Postives = 699/759 (92.09%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSE-GAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSP 60
           MADK+G EQ+VLRIL+ E G  + +KD  K SV S PDF+LKETRS RCTIPQS VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SPEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTS 120
           S EI+RM+P KPPKIP E+  RR SFA SSF+KPKSRL+EPPCP GA+LAE+K  AKST 
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 GSPYN-NSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSG 180
            S    +SPAKITT TSP++ALK+APITPKTPLIG+ G+EEEDDEEVY+TAELKVKE+SG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 RKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWF 240
           ++LK T+++EW+AFLC+TGC IASLTI  L T EIWGLGLW+WCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDIL 300
           INCLVFLIERNFLL+RKVLYFVYGLRKSVIIFIWLALVLLAWGLLFD S KRSK+ N IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIE 360
           NYVTRAL ASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPPL+E
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGR ASTGQLSF+HLKK+SD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENF EEE E+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEE++NVLPLFEG  ETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 VVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIII WLLLMG LTTQ+LVFISSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLR+DNEKIFYPNSVLATKPISN+YRSPEMSDSI+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDK 720
           STSIESIGALKARIK+YLESKPQFWRP +SVVVKEIENVNKMK+ L VNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAP 757
           S+RRSDLVLELKKI EELGIKYHLLPQEV LN V SAAP
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 759

BLAST of MC06g0810 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1233 bits (3190), Expect = 0.0
Identity = 652/765 (85.23%), Postives = 699/765 (91.37%), Query Frame = 0

Query: 1   MADKEGTEQVVLRILDSEGAVDGT-KDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSP 60
           MADK+G EQ+VLRIL+ E  V G+ KD  K SV S PDF+LKETRS RCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SPEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTS 120
           S EI+RM+PLKPPKIP E+  RR SFA SSFSKPKSRL+EPPCP G +LAE+KA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 -GSPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSG 180
            GSP  +SPAKIT  TSP++ALK+ PITPKTPLIG+ G+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 RKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWF 240
           ++LK T+++EWVAFLC+TGC IASLTI  L T EIWGLGLW+WCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDIL 300
           INCLVFLIERNFLL+RKVLYFVYGL+KSVIIFIWLALVLLAWGLLFD S KRSKK N+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIE 360
           NYVTRAL ASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPPL+E
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGR ASTGQLSF+HLK++SD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENF EEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEE++NVLPLFEG  ETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIII WLLLMG LTTQ+LVFISSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLR+DNEKIFYPNSVLATKPISN+YRSPEMSDSI+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDK 720
           STSIESIGALKARIK+YLESKPQFWRP +SVVVKEIENVNKMK+ L VNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSP 763
           S+RRSDLVLELKKI E+LGIKYHLLPQ V LN   SAA  VP  P
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPP 765

BLAST of MC06g0810 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.9e-201
Identity = 381/716 (53.21%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 44  RSIRCTIPQSGVG----SSPSPEIARM--SPLKPPKIPVESTV---RRPSFARSSFSKPK 103
           RS     P+S  G     SPSPEI+++  SP KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGS 163
           SR V+P CP   ++ E++ + +  +G  ++ +        S R     AP+TP   ++  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS---PNNKSNRSVGSPAPVTPSKVVV-- 147

Query: 164 IGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIW 223
              E+++DEE+YK  +L  + RS  K+    LIE   F+ I    +ASLTI+ L+    W
Sbjct: 148 ---EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHTFW 207

Query: 224 GLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLA 283
           GL +W+WCVLV+VIF G L + WF+  +VFLIE NFLLRRKVLYFV+GL+KSV +FIWL 
Sbjct: 208 GLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLC 267

Query: 284 LVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRF 343
           L+L+AW LLF+H  KRS  A  +L  +TR L + L GA  WL+KTLL+KILAA+F    F
Sbjct: 268 LILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNF 327

Query: 344 FDRIQESIFHQYILRTLSGPPLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDV 403
           FDRIQ+S+FHQY+L+TLSG PL+E AERVGR  STG LSF  + K+   G   +++VID+
Sbjct: 328 FDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVIDM 387

Query: 404 DKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFNEEESEQKDKEINSEWEARAA 463
            K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA AA
Sbjct: 388 GKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAA 447

Query: 464 AYQIFRNVAKPGSKYIDEEDLFRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYV 523
           AY +FRNVA+P   YI+EEDL RFM KEEV+ V PLF+GA ETG+I RK    W+V VY 
Sbjct: 448 AYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYT 507

Query: 524 ERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFM 583
            R++LAHSLNDTKTA+++LNKL +A++++V +++WLLL+ + TT++L+F S+QL+ + F+
Sbjct: 508 SRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFI 567

Query: 584 FGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSV 643
            G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+V
Sbjct: 568 IGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAV 627

Query: 644 LATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKE 703
           LATKPISN++RSP M +++EFS+ FST +  I  LK RI  YLE  PQ W P HSVVVKE
Sbjct: 628 LATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKE 687

Query: 704 IENVNKMKMGLYVNHTINFQNYGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           IEN+NK+KM LY +HTI FQ   +++ RR++L L +K++LE+L I Y LLPQ++ L
Sbjct: 688 IENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of MC06g0810 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.9e-201
Identity = 381/716 (53.21%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 44  RSIRCTIPQSGVG----SSPSPEIARM--SPLKPPKIPVESTV---RRPSFARSSFSKPK 103
           RS     P+S  G     SPSPEI+++  SP KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGS 163
           SR V+P CP   ++ E++ + +  +G  ++ +        S R     AP+TP   ++  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS---PNNKSNRSVGSPAPVTPSKVVV-- 147

Query: 164 IGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIW 223
              E+++DEE+YK  +L  + RS  K+    LIE   F+ I    +ASLTI+ L+    W
Sbjct: 148 ---EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHTFW 207

Query: 224 GLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLA 283
           GL +W+WCVLV+VIF G L + WF+  +VFLIE NFLLRRKVLYFV+GL+KSV +FIWL 
Sbjct: 208 GLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLC 267

Query: 284 LVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRF 343
           L+L+AW LLF+H  KRS  A  +L  +TR L + L GA  WL+KTLL+KILAA+F    F
Sbjct: 268 LILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNF 327

Query: 344 FDRIQESIFHQYILRTLSGPPLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDV 403
           FDRIQ+S+FHQY+L+TLSG PL+E AERVGR  STG LSF  + K+   G   +++VID+
Sbjct: 328 FDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVIDM 387

Query: 404 DKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFNEEESEQKDKEINSEWEARAA 463
            K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA AA
Sbjct: 388 GKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAA 447

Query: 464 AYQIFRNVAKPGSKYIDEEDLFRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYV 523
           AY +FRNVA+P   YI+EEDL RFM KEEV+ V PLF+GA ETG+I RK    W+V VY 
Sbjct: 448 AYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYT 507

Query: 524 ERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFM 583
            R++LAHSLNDTKTA+++LNKL +A++++V +++WLLL+ + TT++L+F S+QL+ + F+
Sbjct: 508 SRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFI 567

Query: 584 FGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSV 643
            G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+V
Sbjct: 568 IGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAV 627

Query: 644 LATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKE 703
           LATKPISN++RSP M +++EFS+ FST +  I  LK RI  YLE  PQ W P HSVVVKE
Sbjct: 628 LATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKE 687

Query: 704 IENVNKMKMGLYVNHTINFQNYGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           IEN+NK+KM LY +HTI FQ   +++ RR++L L +K++LE+L I Y LLPQ++ L
Sbjct: 688 IENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of MC06g0810 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.9e-201
Identity = 381/716 (53.21%), Postives = 517/716 (72.21%), Query Frame = 0

Query: 44  RSIRCTIPQSGVG----SSPSPEIARM--SPLKPPKIPVESTV---RRPSFARSSFSKPK 103
           RS     P+S  G     SPSPEI+++  SP KPP+ P ++ V   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGS 163
           SR V+P CP   ++ E++ + +  +G  ++ +        S R     AP+TP   ++  
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS---PNNKSNRSVGSPAPVTPSKVVV-- 147

Query: 164 IGSEEEDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIW 223
              E+++DEE+YK  +L  + RS  K+    LIE   F+ I    +ASLTI+ L+    W
Sbjct: 148 ---EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHTFW 207

Query: 224 GLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLA 283
           GL +W+WCVLV+VIF G L + WF+  +VFLIE NFLLRRKVLYFV+GL+KSV +FIWL 
Sbjct: 208 GLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLC 267

Query: 284 LVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRF 343
           L+L+AW LLF+H  KRS  A  +L  +TR L + L GA  WL+KTLL+KILAA+F    F
Sbjct: 268 LILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNF 327

Query: 344 FDRIQESIFHQYILRTLSGPPLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDV 403
           FDRIQ+S+FHQY+L+TLSG PL+E AERVGR  STG LSF  + K+   G   +++VID+
Sbjct: 328 FDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVIDM 387

Query: 404 DKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFNEEESEQKDKEINSEWEARAA 463
            K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA AA
Sbjct: 388 GKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAA 447

Query: 464 AYQIFRNVAKPGSKYIDEEDLFRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYV 523
           AY +FRNVA+P   YI+EEDL RFM KEEV+ V PLF+GA ETG+I RK    W+V VY 
Sbjct: 448 AYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYT 507

Query: 524 ERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFM 583
            R++LAHSLNDTKTA+++LNKL +A++++V +++WLLL+ + TT++L+F S+QL+ + F+
Sbjct: 508 SRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFI 567

Query: 584 FGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSV 643
            G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+V
Sbjct: 568 IGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAV 627

Query: 644 LATKPISNFYRSPEMSDSIEFSVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKE 703
           LATKPISN++RSP M +++EFS+ FST +  I  LK RI  YLE  PQ W P HSVVVKE
Sbjct: 628 LATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKE 687

Query: 704 IENVNKMKMGLYVNHTINFQNYGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           IEN+NK+KM LY +HTI FQ   +++ RR++L L +K++LE+L I Y LLPQ++ L
Sbjct: 688 IENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of MC06g0810 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 624.8 bits (1610), Expect = 9.3e-179
Identity = 364/755 (48.21%), Postives = 499/755 (66.09%), Query Frame = 0

Query: 8   EQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPSPEIARMS 67
           E+VV+ + D E + D     +   +AS PD +      I  + P   + S P+PEI + S
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-PDSD----AGIEKSKPVPPI-SIPTPEIYKFS 69

Query: 68  PL--KPPKIP-VESTVRRPSFARSSFSKPKSRLVEPPC---------PGGANLAEDKAQA 127
               KPPKIP  E  VRR S +RS +SKPKSR  E             GG +L E     
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDD-EEVYKTAELKVK 187
               GS    SP       S R    +A        +  +  EE D+ EE+YK  +L   
Sbjct: 130 SFARGSFDRASP----NNKSNRSVASAA--------LSKVAEEEPDENEEIYKKVKLHRV 189

Query: 188 ERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLF 247
           +RSG  +K    +E V F+ I G  I SLTI  +    IWGL  W+WCVLV+V   G L 
Sbjct: 190 KRSG--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLV 249

Query: 248 SQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKA 307
           + WF++ +VF+IE+N+LLR+KVLYFV+GL+K+V +FIW +LVL+AW  LFD   KR++K 
Sbjct: 250 TNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKT 309

Query: 308 NDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 367
              L+++T  + + L G+ L+L+KT  +K+LA+ F    FF+RIQES+FHQY+L+TLSGP
Sbjct: 310 KRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGP 369

Query: 368 PLIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 427
           PLIE AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI
Sbjct: 370 PLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLI 429

Query: 428 NVIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 487
             + +SG+STIS+T++  N ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL
Sbjct: 430 EAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDL 489

Query: 488 FRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 547
            RFM KEEV+ VLPL E A +TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+K
Sbjct: 490 LRFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDK 549

Query: 548 LASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 607
           L + ++ ++  IVW++L+ + +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP+
Sbjct: 550 LITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPY 609

Query: 608 DVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 667
           DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D ++F
Sbjct: 610 DVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDF 669

Query: 668 SVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQN 727
            + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ + + V HTINFQ 
Sbjct: 670 GIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQV 729

Query: 728 YGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYL 750
           Y +KS RR+ L++ +K+ILE+L I Y LLPQ+V L
Sbjct: 730 YVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of MC06g0810 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 531.9 bits (1369), Expect = 8.2e-151
Identity = 306/712 (42.98%), Postives = 442/712 (62.08%), Query Frame = 0

Query: 92  KPKSRLVEPPCPGGANLAEDKAQAKSTSGSPYNNSPAKITTATSPRDALKSAPITPKTPL 151
           K +SRL++PP P   ++   +          ++    K                 P TP 
Sbjct: 185 KTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTK-----------------PGTPN 244

Query: 152 IGSIGSEE-EDDEEVYKTAELKVKERSGRKLKITILIEWVAFLCITGCFIASLTIHKLQT 211
            G  GS++ E++E+ +   +L    R   K+ + ++IEW+  + I    I SL I  L+ 
Sbjct: 245 QG--GSKDLEEEEDPFSEEDLPEGLRK-EKICVWVIIEWIFLILIIASLICSLVIPYLRG 304

Query: 212 TEIWGLGLWQWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIF 271
             +W L LW+W V+VLV+ CGRL S W +   V+ +E NFL R+KVLYFVYG+RK V   
Sbjct: 305 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 364

Query: 272 IWLALVLLAWGLLFDHSRKRSKKANDILNYVTRALAASLAGAGLWLLKTLLVKILAASFQ 331
           +WL LVL+AW  LFD   +R  ++  +L YVT+ L   L    +WL+KTLLVK+LA+SF 
Sbjct: 365 LWLGLVLIAWHFLFDKKVEREMRST-VLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFH 424

Query: 332 CTRFFDRIQESIFHQYILRTLSGPPLIEM---AERVGRTASTGQLSFRHLK--------- 391
            + +FDRIQES+F QY++ TLSGPP IE+    E+V     T ++  R L          
Sbjct: 425 MSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSS 484

Query: 392 ------------------------KQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 451
                                    +S    EG EE I +D L++M  + +SAW M+ L+
Sbjct: 485 PPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLM 544

Query: 452 NVIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 511
           NVI+   LST+   I++   +E + K  +I SE+EA+ AA +IF+NVA+PGS+YI  ED 
Sbjct: 545 NVIKKGTLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDF 604

Query: 512 FRFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 571
            RF+S++E E  + LFEGA E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L++
Sbjct: 605 MRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHR 664

Query: 572 LASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPF 631
           +   +V IVI+I+WLL++G+ TT+ L+ ISSQLLLVVF+FGN+ +T+FEA+IFVFVMHPF
Sbjct: 665 IVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPF 724

Query: 632 DVGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEF 691
           DVGDRC +DGVQM+VEEMNILTT+FLRFDN+KI YPNS+L TKPI+N+YRSP+M D+IEF
Sbjct: 725 DVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEF 784

Query: 692 SVDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQN 751
            V  +T  E   AL+ RI SY+++K   W P   +V +++  +N +K+ ++  H +N QN
Sbjct: 785 FVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQN 844

Query: 752 YGDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPM----VPPS 763
            G++  RR  L+ E+ ++  EL I+Y L P  + + S+ +A P+    +PPS
Sbjct: 845 MGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPS 874