MC06g0746 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0746
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionvacuolar protein sorting-associated protein 32 homolog 2
LocationMC06: 6178443 .. 6181486 (-)
RNA-Seq ExpressionMC06g0746
SyntenyMC06g0746
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTAAGCCCAACATTGAAAACGGCAACTCGTAGGCCCATCTCTTCATCCATCAGCCAAGCCCAATACTTAAACGTTAACTCGGTCGGCCCATCTAAAGATAAGCTCGCGACTTGCTCGGCCCATTTTCACTAATAATCTAAGCCCAATACTAGAGACGGCAACTGGAGTCATACTTTCACCGTCTCAAAATCGGATGTAGCTGTAGTTTGTGCTGTGTTTCGTGGGACCAATCAGCTCTGATCGACGTGGAATCCAACGACCCTGTCAACGCCGTGAAGAAAAGGCAATGAACCTTCCTCTTTCTCCCATTCCACACTACAACAACACGCCTAAGCAAAACCCGAATCAAATTCACCTCTCTTGCAGATCTTCCCTTGTTCTTTAATCCTGTTTTGCGACACCGATCGCTCTTGGATTTCCTCTCTTCAGTTCTCAGGTACACACATGTATTCATCAAATTTTTGTATTTTTAGTTTGGTAATTGACCGTCCGTTTTCAATCCTGATTCTGGTAGAATTTCGACAATTGATTTCTGCTCAGACGAGACACGAGTGATTATTTTCTTGCTATTGATGCAGAAGTTCGGGGGTGGGTTCGAATTCGCAGACAATCCACCATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTACGGCTCTGGCGACGCTTGATAAATTAAACGAGGTATCGTGGCGTGCTGAGTATCTTTCCAGTTGAATTCCGCTTCTCTTTTGTCTTTGAATGGATTCTGGAGTGTTTTTTGAACCACTAATTTGGCTAGGTCGAGAGGTGGAGTGAAAGATCTTTTAGAATTAGTGACGCGATCGATTTTTTGCTTTTGAGCGATGTGAACATCACTAATTTCTGTCCTTTGATATTATGAATTCTTTATGCTGAAATGAATTCTTTTATGCTCTGTGTTTTTCAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCGGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAAAACAAGCGGGGTATGATTGTAAGAAAGTACTAGCAGTTTACTATTGAGCAAAACATTTTGGCGTTAAAGTACTTGTTTATTATATAGAATGATGCGACTGAACTTGCTAACTGTTGATAATTAACGTCAAGAGTGGCATAGCAATTAAAACAGTGTGGGTTCAAAGGAAGTAACTTATTAATTTGGTTAGCAAGTTCAGCCACAGTTAGCATTTTAGCATTTAAAATTGTGCCTATAAAGTTGGGGGATTTCTAGGTCAAAGGAAGTACCTTATTTGGTTACTGTCAGGTTTTAACTATCCATGTTCCAGGTCGAAGGAAGTAACTTATGCTTCTATTTGGATTTTTGGCTTTGCTAGTTGTGTTTGTTACATGCCCAAATTGGCGTTGCGGTTCATTTTCACTGTTGGTGAAAGATAATAATTTGCTTATACTTGTTCATTTTCATTTCTCACATCTCCCATCACAATTTCTAGAATCCTCATCGTTAACTTCTTCTGTTTCAAGTACTTGTGTGTTTTGGAGGAGATATTTATGACATTGGATCTTTCCCACATTTCATTTGCAATTTTAATCTCGCTCAGTTGAGCATCAGCTATTTTATGGTGTGAGATTCAAACTATAGTTGGCAATGTTGCGTCAAAATTAACTATTTTTATTTTATAGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAGCAGATAGAGCAGCTTGGCAACTTCCAATTGCGGATCCATGATCAGGTGATCTTTGATGTCATATCTGATATCAGACTTGTAAAACGTATCCATATTTGCTTTGCTGTGTGTTTATTTAACTTTGTGAGTCATAAATATTCGTGTTGCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACCGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGTGAGTAACACATTGTTTGGGTGTAATTTCATGATTTGCTCTTTATGACCGTTTTCAATCCGTTCATATTTTTCAGTTCTCTTCTCCTACCCAGAGGACTGAATCTTGTGAATAATGTGCTGGGGAGCATAATGATGCATGAAACCTGAATTTTTCCATAATATCTACGATATTGCATGTTTAGTGCTTTATAAGGATTACAGAAATGAACTAAGAATCATTTTTTGTACAAATATCAACCTGATTGGTTGAAATACTTCTTTTGCAGGAATATCGATGATGTGGATAAGACGATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCCTCAGCTGATTTTGATGAGGTTTTTATTCACTTTCAGTGTGTTTTTTCCGTTTGCCCTGCGAGTTTGTGAAGTTTTTTTCATTCACTAAACCTCTTTGTACGCACAGGATGAACTGGAAGCAGAGCTTGAAGAGCTAGAAGGTGCTGAGTTGGAGGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCCCCAATTCCTGTCCCAGCGGGCAAACAACCTGCTCGACCTGCTCCACAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGAGTTGATATCCTTCATGGTTTTCAAGCAGGTGCTTGGGCTTATCTTGTGATGAATTCTTTCCATTTGTTAGTGTCATTTGCCTATGAAATGTTTATAGATCGTATGATGCTTGAAAACACTTTGCGCATTGTACCTAAAGCTGTCAAGACTAAGATACTTTTATGGAACTTTTCAGGTCGCATTGCCTGATGAATATTGGAAGCGAGAGGCTATGTGAAGAATTGGCCTGAGGTTGTAACAAAGATTTGAACATTTTTTGCTAAGTTCAGCGAAACCTGATGAACAGGTTTCTTTGTGATAATACCAGATAATAATCCGAATTCAATCTTAGGTCCTGTATACCATGTAAATTAATATACCACTACTATGGTTTCGCGTTGGCCTAATATGGAGTTAATTTGTAAGTGCCT

mRNA sequence

AGCTAAGCCCAACATTGAAAACGGCAACTCGTAGGCCCATCTCTTCATCCATCAGCCAAGCCCAATACTTAAACGTTAACTCGGTCGGCCCATCTAAAGATAAGCTCGCGACTTGCTCGGCCCATTTTCACTAATAATCTAAGCCCAATACTAGAGACGGCAACTGGAGTCATACTTTCACCGTCTCAAAATCGGATGTAGCTGTAGTTTGTGCTGTGTTTCGTGGGACCAATCAGCTCTGATCGACGTGGAATCCAACGACCCTGTCAACGCCGTGAAGAAAAGATCTTCCCTTGTTCTTTAATCCTGTTTTGCGACACCGATCGCTCTTGGATTTCCTCTCTTCAGTTCTCAGAAGTTCGGGGGTGGGTTCGAATTCGCAGACAATCCACCATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTACGGCTCTGGCGACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCGGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAAAACAAGCGGGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAGCAGATAGAGCAGCTTGGCAACTTCCAATTGCGGATCCATGATCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACCGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATCGATGATGTGGATAAGACGATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCCTCAGCTGATTTTGATGAGGATGAACTGGAAGCAGAGCTTGAAGAGCTAGAAGGTGCTGAGTTGGAGGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCCCCAATTCCTGTCCCAGCGGGCAAACAACCTGCTCGACCTGCTCCACAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGAGTTGATATCCTTCATGGTTTTCAAGCAGGTCGCATTGCCTGATGAATATTGGAAGCGAGAGGCTATGTGAAGAATTGGCCTGAGGTTGTAACAAAGATTTGAACATTTTTTGCTAAGTTCAGCGAAACCTGATGAACAGGTTTCTTTGTGATAATACCAGATAATAATCCGAATTCAATCTTAGGTCCTGTATACCATGTAAATTAATATACCACTACTATGGTTTCGCGTTGGCCTAATATGGAGTTAATTTGTAAGTGCCT

Coding sequence (CDS)

ATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTACGGCTCTGGCGACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCGGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAAAACAAGCGGGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAGCAGATAGAGCAGCTTGGCAACTTCCAATTGCGGATCCATGATCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACCGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATCGATGATGTGGATAAGACGATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCCTCAGCTGATTTTGATGAGGATGAACTGGAAGCAGAGCTTGAAGAGCTAGAAGGTGCTGAGTTGGAGGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCCCCAATTCCTGTCCCAGCGGGCAAACAACCTGCTCGACCTGCTCCACAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGA

Protein sequence

MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAPAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL
Homology
BLAST of MC06g0746 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 1.7e-93
Identity = 189/219 (86.30%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M N+LFGKPKQE+ AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-IPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P +PVPAG+QPARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of MC06g0746 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 6.3e-85
Identity = 182/217 (83.87%), Postives = 196/217 (90.32%), Query Frame = 0

Query: 3   NKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQC 62
           N+LFGKPKQE++ L TLDKLNETLEMLEKKE VLLKKA  EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAP 182
           DKTMDEINEQTENMKQIQEALS P GA+ DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFGAN-DFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPIPVPAGKQPAR-PAPQKRTAEEDELAALQAEMAL 219
             PI VP G +PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of MC06g0746 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 89/217 (41.01%), Postives = 125/217 (57.60%), Query Frame = 0

Query: 1   MFNKLF---GKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKR 60
           +F K+F   GK  + ++    + +L ET EML KK++ L KK   E+  AK     KNKR
Sbjct: 3   LFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQP 180
           +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEI 182

Query: 181 ATTAPAAPIPVPAGKQPARPAPQKRTAEEDELAALQA 215
               P     VP+   P+RPA +K   +ED++  L+A
Sbjct: 183 GDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of MC06g0746 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-25
Identity = 87/217 (40.09%), Postives = 125/217 (57.60%), Query Frame = 0

Query: 1   MFNKLF---GKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKR 60
           +  KLF   GK  +  +    + KL +T EML KK++ L KK   E+  AK +   KNKR
Sbjct: 3   LIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQP 180
           +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLE- 182

Query: 181 ATTAPAAPIP-VPAGKQPARPAPQKRTAEEDELAALQ 214
                  P+P VPA   PA+P  +K+  ++D++  L+
Sbjct: 183 VQGPETVPLPNVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of MC06g0746 vs. ExPASy Swiss-Prot
Match: Q6GL11 (Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.6e-25
Identity = 87/217 (40.09%), Postives = 125/217 (57.60%), Query Frame = 0

Query: 1   MFNKLF---GKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKR 60
           +  KLF   GK  +  +    + KL +T EML KK++ L KK   E+  AK +   KNKR
Sbjct: 3   LIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLTKKQEFLEKKIEQELVTAKKH-GTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQP 180
           +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLE- 182

Query: 181 ATTAPAAPIP-VPAGKQPARPAPQKRTAEEDELAALQ 214
                  P+P VPA   PA+P  +K+  ++D++  L+
Sbjct: 183 VQGPETVPLPNVPAAVLPAKPVKKKQEEDDDDMRELE 217

BLAST of MC06g0746 vs. NCBI nr
Match: XP_022144946.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Momordica charantia] >XP_022144947.1 vacuolar protein sorting-associated protein 32 homolog 2 [Momordica charantia])

HSP 1 Score: 394 bits (1011), Expect = 2.00e-137
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of MC06g0746 vs. NCBI nr
Match: XP_008450809.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008450810.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_016900986.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >TYK10128.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 383 bits (983), Expect = 3.97e-133
Identity = 212/218 (97.25%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M NKLFGKPKQES ALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRAAI
Sbjct: 3   MLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. NCBI nr
Match: XP_004135686.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011659962.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031737801.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN66200.1 hypothetical protein Csa_006989 [Cucumis sativus])

HSP 1 Score: 383 bits (983), Expect = 3.97e-133
Identity = 212/218 (97.25%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQES ALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRAAI
Sbjct: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELE AELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. NCBI nr
Match: XP_022961241.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961242.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961243.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961244.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022987856.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987857.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987858.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987859.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >KAG7023632.1 Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 383 bits (983), Expect = 3.97e-133
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQES ALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           AP APIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. NCBI nr
Match: XP_038880238.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida])

HSP 1 Score: 382 bits (980), Expect = 1.14e-132
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQES ALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPI VPAG+Q ARP PQKRTAEEDELAALQAEMAL
Sbjct: 183 APAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. ExPASy TrEMBL
Match: A0A6J1CUX2 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Momordica charantia OX=3673 GN=LOC111014495 PE=4 SV=1)

HSP 1 Score: 394 bits (1011), Expect = 9.67e-138
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of MC06g0746 vs. ExPASy TrEMBL
Match: A0A6J1JBI2 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485275 PE=4 SV=1)

HSP 1 Score: 383 bits (983), Expect = 1.92e-133
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQES ALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           AP APIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. ExPASy TrEMBL
Match: A0A1S3BQS1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103492284 PE=4 SV=1)

HSP 1 Score: 383 bits (983), Expect = 1.92e-133
Identity = 212/218 (97.25%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M NKLFGKPKQES ALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRAAI
Sbjct: 3   MLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. ExPASy TrEMBL
Match: A0A6J1H9T9 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461810 PE=4 SV=1)

HSP 1 Score: 383 bits (983), Expect = 1.92e-133
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           MFNKLFGKPKQES ALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRAAI
Sbjct: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           AP APIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. ExPASy TrEMBL
Match: A0A5D3CFX2 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002710 PE=4 SV=1)

HSP 1 Score: 383 bits (983), Expect = 1.92e-133
Identity = 212/218 (97.25%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M NKLFGKPKQES ALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRAAI
Sbjct: 3   MLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID
Sbjct: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPATT
Sbjct: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182

Query: 181 APAAPIPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 218
           APAAPIPVPAG+Q ARPAPQKRTAEEDELAALQAEMAL
Sbjct: 183 APAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of MC06g0746 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.2e-94
Identity = 189/219 (86.30%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M N+LFGKPKQE+ AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-IPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P +PVPAG+QPARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of MC06g0746 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.2e-94
Identity = 189/219 (86.30%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M N+LFGKPKQE+ AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-IPVPAGKQPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P +PVPAG+QPARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of MC06g0746 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 315.1 bits (806), Expect = 4.5e-86
Identity = 182/217 (83.87%), Postives = 196/217 (90.32%), Query Frame = 0

Query: 3   NKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQC 62
           N+LFGKPKQE++ L TLDKLNETLEMLEKKE VLLKKA  EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAP 182
           DKTMDEINEQTENMKQIQEALS P GA+ DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFGAN-DFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPIPVPAGKQPAR-PAPQKRTAEEDELAALQAEMAL 219
             PI VP G +PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of MC06g0746 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 301.2 bits (770), Expect = 6.7e-82
Identity = 167/192 (86.98%), Postives = 180/192 (93.75%), Query Frame = 0

Query: 28  MLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 87
           MLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  ILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPI 147
           I+LEGAKATTETVDALR+GA+AMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GASADFDEDELEAELEELEGAELEEQLLQPATTAPAAP-IPVPAGKQPARPAPQKRTAEE 207
           GA+ADFDEDEL AEL+ELE  ELE QLLQPATTAP  P +PVPAG+QPARP PQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of MC06g0746 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-06
Identity = 61/220 (27.73%), Postives = 95/220 (43.18%), Query Frame = 0

Query: 1   MFNKLFGKPKQESTALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 60
           M N    KPK      A L  L      L + ++ L K    E + A+D  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 120
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G  A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 180
           DV K MD+  E      ++   L   + A    DE+E+ AE + LE   + E + +  TT
Sbjct: 121 DVQKLMDDTAEAKAYQDELSAILGEKLSAE---DEEEILAEFDNLESLLIVEDMPEVPTT 180

Query: 181 --APAAP-------IPVPAGKQPARPAPQKRTAEEDELAA 212
              P  P       +P  A          KR   E+ L A
Sbjct: 181 ELMPEEPEKMDLPDVPTKAPVASNETTSTKRKVLEEPLEA 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZE41.7e-9386.30Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821976.3e-8583.87Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC43.9e-2641.01Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q5XGW63.3e-2540.09Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Q6GL115.6e-2540.09Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b P... [more]
Match NameE-valueIdentityDescription
XP_022144946.12.00e-137100.00vacuolar protein sorting-associated protein 32 homolog 2 [Momordica charantia] >... [more]
XP_008450809.13.97e-13397.25PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
XP_004135686.13.97e-13397.25vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
XP_022961241.13.97e-13397.25vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
XP_038880238.11.14e-13297.25vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1CUX29.67e-138100.00vacuolar protein sorting-associated protein 32 homolog 2 OS=Momordica charantia ... [more]
A0A6J1JBI21.92e-13397.25vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxim... [more]
A0A1S3BQS11.92e-13397.25vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A6J1H9T91.92e-13397.25vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A5D3CFX21.92e-13397.25Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
Match NameE-valueIdentityDescription
AT4G29160.11.2e-9486.30SNF7 family protein [more]
AT4G29160.31.2e-9486.30SNF7 family protein [more]
AT2G19830.14.5e-8683.87SNF7 family protein [more]
AT4G29160.26.7e-8286.98SNF7 family protein [more]
AT5G09260.11.6e-0627.73vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableGENE3D1.10.287.1060coord: 13..91
e-value: 2.0E-21
score: 77.9
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..172
e-value: 5.0E-9
score: 37.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..207
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761:SF63VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 1coord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 4.4E-46
score: 156.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0746.1MC06g0746.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body