MC06g0719 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0719
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMalic enzyme
LocationMC06: 5913178 .. 5917694 (+)
RNA-Seq ExpressionMC06g0719
SyntenyMC06g0719
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATGGATTTTTATTATTTAATGGGGACCCAAGTCTTCTGTTCTTGTGTTGCGTTTGTTTTTTGGGTGGACTTGCTTCTTCTGCTACTTTCTCTGAAGGGGTTCGATCGGTTGAGTGATCGGGATGGAGAGCACTTTGAAGGAGATCGGAGATGGCGGTTCGGTGCTCGACTTGGACCCTAAATCCACCGTCGGCGGTGGGGTTGAGGATGTCTATGGAGAAGACTGTGCTACAGAGGAGCAGCTGATCACTCCATGGACTTTCTCAGTTGCTAGGTGAATTCAACTTTATTGGATTTTTGGGTGTGATTATTGCTTTCTTTTGCTGACTTTGGTTGAGTGTTTATGTTGCTAATTTGTGCTTAATGAGACATGCAACCGTTTTGTTTGACTGATCACTTACTACGAACTCTGTTTTTCTCTGTTTTTCCCACCTGTTTTCAAATTCCGGGAACTACTTGCTTTCTCTTTCCCTTTCATATTGTGACGTTTTATCTCTAGGATTTGTGATAGTGAGATATTATCGATCCTTGAAAAAGAGTCATTGTAGGAGACTTTTGATTCTTCCTCGAGATTCTTATTAAGCTGGCCTCTAGATCAGGGGCCGTTGAGTTGGATTCTAGGAATATTACCATTCACTGTAAAATGAAGAATGTTATTCTCAAAATTAAACATAAATCATTTTTGCACTCTCTGATATGATGCAGTGGCTATTCACTGCTGAGGGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGTCTTCTGCCACCTTCCATTGTTACTCAACAACTTCAGGTTGCTGCTGATGTCCCATTATGATGTTTTGTTTTTTCTTGGTCATGACCTTTTCCATTTCTGTAATCAGGAGAAGAAGTTGATGCAAAACATCAGACAGTATCAGCTTCCACTTCAAAAATTTATTGCCATGATGGAACTTCAGGTTCTGACTTTACCTACTGTCCTCAGTTAACTTGCTGATAATTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTCCGTTTGAACCTCACTTTTGTTTACCAGAATAAGTGGAAAAGAGTAATCCAATGGCTCAACCATTTTGATTAGTAGAGCTTGTATTTCGTTGTACAGGAAAGGAATGAAAGACTTTTTTACAAACTTCTTATTGACAATGTCGAAGAACTGCTTCCCGTTGTCTATACTCCCACTGTCGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGGCCTCAGGGTCTTTACATTAGTTTGAAAGAGAAGTAAGGATGCCTTTCTTACTAAATATGTTTACAACTTTCAAGGTTTTGTGAATCTATTAACGCCACGTCGTGCAGGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGAAGTATCCAAGTAATTGTTGTGACCGATGGTGAGCGCATTTTGGGTCTTGGTGATCTTGGTTGTCAGGTAATTCCTATGGAAATATTCTTTAATGAATTTGATCTACTTTACTAGCATATTTACTGACTTTGATATGAATTTATTGGATGGTTTAGGGAATGGGAATTCCAGTTGGGAAACTTTCTCTATACACCGCACTTGGAGGAGTTCGTCCTTCAGCAGTAAGGATTTATTTAAAAAACAAAATTGCCTGTTATTTTTTTGTCATGAATTTATTTTGATGCTTCAGCAGAAACTTTCTTTACTCACATTACATTGGGAATCACGAAACTTTATCTAGCTTAGTTTTAAGATCTGAAGGCCATTCTTCAATTCAACTAATGCCACATTTTCTTGATAGTGTTTGCCTATTACGATTGATGTCGGGACAAACAACGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAGAGGTTCAGCATGTGTCCTTATCTTATTTTTTGCCGGTTTGAGTACATTTGAGTTATTTTAAGTTTGTGAGTTTATAACAAAACTCAAGTATTTTGCCTATGTTTTATGCAGGAGTACTATGAACTTTTACATGAGTTTATGACTGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGGTTAGAACATGATATTTGATATATAATTCTAGTTATTGGTAATTGAGGCTTGAATCATTTCTGTACTCACTGCTTCTAACCGAAACTTTCAGTTTGAAGATTTTGCCAACCACAATGCTTTTGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTCTTCAATGATGATATTCAGGTACAGGGTAACATAAATTTGATACTCATCATGTAGTAAGTTTTCAAGTTTTCTTATCCATACTCTTTTCCTGATTCCAGGGGACAGCATCTGTCGTTCTTGCGGGCGCTGTTTCTGCCCTGAAACTCATTGGTGGTACCTTGGCCGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGTAAGATCTCTCCATTCTTCCCTTTATTTTCATAAATTCCTTTCCATAAATGGTCATATTTCCCGAAACAGTATTATTTCCTTTCAACTCCACTTTTTCTCATTCATGTTCAAATGATTCTTACATATTCTTGGCTTGGCTACAGGCTGGAACTGGTATAGCAGAGCTTATAGCTCTTGAAATATCAAAAAAGGTACTTGTCAAGCGATGCCAAAATAGATTACAAACACATTTCGTAACCTATGTTTCTTATAATCTTTTCCTTTCTTTAGACAAATGTTCCAGTGGAGGAGACCCGCAAGAAGATCTGGCTTGTTGACTCGAAGGTAGTAGAATGTTTAATCAAGTTTATAAGAGAATCGTAATTATTTCTTGATGAGTTATTGTTTGTTCTTGGCTTCTTGCTTCAACTATATAGAGAATGGCTTTTCTGTAGGGATTAATTGTTCACTCCCGCAAAGATTCACTTCAACACTTTAAGAAGCCATGGGCTCATGAGCATGAACCTGTTCAGGATTTGTTAAGCGCTGTCAAGGTGTGAACCATTCTCGATCTTTTTGAGTTTCAATTGCATCCCATAAATCTCACAATGGTTCCTTGTCGCTTGCCTATTTCTCATTATCTCAGGCAATTAAGCCAACAGTTTTGATTGGATCATCTGGAGTTGGAAGAACTTTTACGAAAGAAGTCGTTGAGGCCGTGTCCTCCATAAATGAGGTTCTTTCTTCTCCCTCACAGATGCACTCATAAGCACATATACATATTAGATACATGTTTAGAAATAATTTTCAAATTAGGATTGACTCGAGCAAAATGTATTCCCCTGAAAGCAAACTGACCTTTCTTTGTTGTATTGTTATGCAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACAATTGGAGTGAGGTATATAACTAACATTCCATTTTAGACGGCAAGTTCTTCTTTCTCTCTCTTTTTCCACGAGTCTTCATTTTCTATTTACTTTGCTTTTTTATGCAAATTTAAGGGCCGTGCAATCTTCGCTAGCGGAAGTCCATTCGATCCAGTCGAATACAATGGGAAGACCTTTGTCCCTGGCCAGGTTTTTCTGCTTAATCATATTATGTTGGATGCTTTACACTTCAGTTCCATTTTCACATATCAAAAGCTGACATCTTGAACTTGTCAAATCTTAAATCAGGCCAACAATGCCTACATATTCCCTGGGTTTGGTTTGGGTGTCGTAATTTCTGGAGCTATCCGTGTGCACGACGACATGCTTCTGGCTGCCTGTAAGTAGGAGGGTTGTTAAATAAGCAATATGACACTACAATACATACATAACATACACTCGTATATAAAGTCCAAACACTAACCATACCATAAACCATAATCTATGAACTTGAAACCCTTTTGTTGATAAACAGCAGGCTCCCTGACTTGTACAATAACAATGTTTGACCTTTAAATGATGATACAAGGAATGATTTTTATTCTATGTTTGTCGAACCAGCGGAAGCCTTGGCTGCGCAAGTCTCTGCGGAAAACTACGAGAAGGGATTGATCTACCCGCCCTTCACCAACATCCGAAAGATCTCGGCTCACATAGCAGCTAACGTGGCTGCCAAGGCATATGAACTTGGTGAGACATTTTCTTGGATCATGTTTCTATTCTTGTAGTTGTGAGTCCTCCATAGTTTTATCTAAAGTGGAATCCTAATTTCCCCCCTTTTACGTACTCTCCAGGCTTGGCGACCCGTCTCCCGCGCCCTGCCGATCTTATGAAGTATGCCGAGAGCTGCATGTACAGCCCCGTCTACCGAACCTATCGATAAATGTAGCGAAGTTGCGACTGTGGTTTGTATTATAGAGCTTTTCATGAAAGCATTGTGTAATGCTGTATGGTGTGTGCCTGGGAGAGAGCTTGAAGTCTTCCAAAATTTGAATTGTAATGCTGTAATGTTTTCCTTCAGAAGTGGACTTAATTTGTTAATGAAACAAACTGTTTTTGTATCAATTTTCAAGTGCTTCAATCCTACCAATTTTCAAGCTTTTG

mRNA sequence

CGATGGATTTTTATTATTTAATGGGGACCCAAGTCTTCTGTTCTTGTGTTGCGTTTGTTTTTTGGGTGGACTTGCTTCTTCTGCTACTTTCTCTGAAGGGGTTCGATCGGTTGAGTGATCGGGATGGAGAGCACTTTGAAGGAGATCGGAGATGGCGGTTCGGTGCTCGACTTGGACCCTAAATCCACCGTCGGCGGTGGGGTTGAGGATGTCTATGGAGAAGACTGTGCTACAGAGGAGCAGCTGATCACTCCATGGACTTTCTCAGTTGCTAGTGGCTATTCACTGCTGAGGGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGTCTTCTGCCACCTTCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAAAACATCAGACAGTATCAGCTTCCACTTCAAAAATTTATTGCCATGATGGAACTTCAGGAAAGGAATGAAAGACTTTTTTACAAACTTCTTATTGACAATGTCGAAGAACTGCTTCCCGTTGTCTATACTCCCACTGTCGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGGCCTCAGGGTCTTTACATTAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGAAGTATCCAAGTAATTGTTGTGACCGATGGTGAGCGCATTTTGGGTCTTGGTGATCTTGGTTGTCAGGGAATGGGAATTCCAGTTGGGAAACTTTCTCTATACACCGCACTTGGAGGAGTTCGTCCTTCAGCATGTTTGCCTATTACGATTGATGTCGGGACAAACAACGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAGAGGAGTACTATGAACTTTTACATGAGTTTATGACTGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAAGATTTTGCCAACCACAATGCTTTTGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTCTTCAATGATGATATTCAGGGGACAGCATCTGTCGTTCTTGCGGGCGCTGTTTCTGCCCTGAAACTCATTGGTGGTACCTTGGCCGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGCTGGAACTGGTATAGCAGAGCTTATAGCTCTTGAAATATCAAAAAAGACAAATGTTCCAGTGGAGGAGACCCGCAAGAAGATCTGGCTTGTTGACTCGAAGGGATTAATTGTTCACTCCCGCAAAGATTCACTTCAACACTTTAAGAAGCCATGGGCTCATGAGCATGAACCTGTTCAGGATTTGTTAAGCGCTGTCAAGGCAATTAAGCCAACAGTTTTGATTGGATCATCTGGAGTTGGAAGAACTTTTACGAAAGAAGTCGTTGAGGCCGTGTCCTCCATAAATGAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACAATTGGAGTGAGGGCCGTGCAATCTTCGCTAGCGGAAGTCCATTCGATCCAGTCGAATACAATGGGAAGACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATATTCCCTGGGTTTGGTTTGGGTGTCGTAATTTCTGGAGCTATCCGTGTGCACGACGACATGCTTCTGGCTGCCTCGGAAGCCTTGGCTGCGCAAGTCTCTGCGGAAAACTACGAGAAGGGATTGATCTACCCGCCCTTCACCAACATCCGAAAGATCTCGGCTCACATAGCAGCTAACGTGGCTGCCAAGGCATATGAACTTGGCTTGGCGACCCGTCTCCCGCGCCCTGCCGATCTTATGAAGTATGCCGAGAGCTGCATGTACAGCCCCGTCTACCGAACCTATCGATAAATGTAGCGAAGTTGCGACTGTGGTTTGTATTATAGAGCTTTTCATGAAAGCATTGTGTAATGCTGTATGGTGTGTGCCTGGGAGAGAGCTTGAAGTCTTCCAAAATTTGAATTGTAATGCTGTAATGTTTTCCTTCAGAAGTGGACTTAATTTGTTAATGAAACAAACTGTTTTTGTATCAATTTTCAAGTGCTTCAATCCTACCAATTTTCAAGCTTTTG

Coding sequence (CDS)

ATGGAGAGCACTTTGAAGGAGATCGGAGATGGCGGTTCGGTGCTCGACTTGGACCCTAAATCCACCGTCGGCGGTGGGGTTGAGGATGTCTATGGAGAAGACTGTGCTACAGAGGAGCAGCTGATCACTCCATGGACTTTCTCAGTTGCTAGTGGCTATTCACTGCTGAGGGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGTCTTCTGCCACCTTCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAAAACATCAGACAGTATCAGCTTCCACTTCAAAAATTTATTGCCATGATGGAACTTCAGGAAAGGAATGAAAGACTTTTTTACAAACTTCTTATTGACAATGTCGAAGAACTGCTTCCCGTTGTCTATACTCCCACTGTCGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGGCCTCAGGGTCTTTACATTAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGAAGTATCCAAGTAATTGTTGTGACCGATGGTGAGCGCATTTTGGGTCTTGGTGATCTTGGTTGTCAGGGAATGGGAATTCCAGTTGGGAAACTTTCTCTATACACCGCACTTGGAGGAGTTCGTCCTTCAGCATGTTTGCCTATTACGATTGATGTCGGGACAAACAACGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAGAGGAGTACTATGAACTTTTACATGAGTTTATGACTGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAAGATTTTGCCAACCACAATGCTTTTGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTCTTCAATGATGATATTCAGGGGACAGCATCTGTCGTTCTTGCGGGCGCTGTTTCTGCCCTGAAACTCATTGGTGGTACCTTGGCCGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGCTGGAACTGGTATAGCAGAGCTTATAGCTCTTGAAATATCAAAAAAGACAAATGTTCCAGTGGAGGAGACCCGCAAGAAGATCTGGCTTGTTGACTCGAAGGGATTAATTGTTCACTCCCGCAAAGATTCACTTCAACACTTTAAGAAGCCATGGGCTCATGAGCATGAACCTGTTCAGGATTTGTTAAGCGCTGTCAAGGCAATTAAGCCAACAGTTTTGATTGGATCATCTGGAGTTGGAAGAACTTTTACGAAAGAAGTCGTTGAGGCCGTGTCCTCCATAAATGAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACAATTGGAGTGAGGGCCGTGCAATCTTCGCTAGCGGAAGTCCATTCGATCCAGTCGAATACAATGGGAAGACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATATTCCCTGGGTTTGGTTTGGGTGTCGTAATTTCTGGAGCTATCCGTGTGCACGACGACATGCTTCTGGCTGCCTCGGAAGCCTTGGCTGCGCAAGTCTCTGCGGAAAACTACGAGAAGGGATTGATCTACCCGCCCTTCACCAACATCCGAAAGATCTCGGCTCACATAGCAGCTAACGTGGCTGCCAAGGCATATGAACTTGGCTTGGCGACCCGTCTCCCGCGCCCTGCCGATCTTATGAAGTATGCCGAGAGCTGCATGTACAGCCCCGTCTACCGAACCTATCGATAA

Protein sequence

MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR
Homology
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BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 7.7e-303
Identity = 514/591 (86.97%), Postives = 558/591 (94.42%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLK+I DG SVLDLDPK+TVGGGVED+YGED ATE+QL+TPWT SVASGYSLLRDP 
Sbjct: 1   MESTLKDIRDGASVLDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDPR 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAF +KERDAHYL GLLPP + TQ+LQE+KLM +IRQYQ+PLQK++AMM+LQERNE
Sbjct: 61  HNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWP+R
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPER 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
            IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 RIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
            +EFYIGLKQRRATG+EY E L EFM+ VKQNYGEKVLIQFEDFANHNAF+LLAKY T+H
Sbjct: 241 ANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           L FNDDIQGTASVVLAG VSAL+L+GGTLADH FLFLGAGEAGTGIAELIALE+SK+T  
Sbjct: 301 LAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTKC 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWLVDSKGLIV SRKDSLQ FKKPWAHEHEPV+DLL AVK IKPTVLIGSSG
Sbjct: 361 PIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VG+ FTKEV+EA++S NEKPLI+ALSNPTSQSECTAEEAY W++GRAIFASGSPFDPVEY
Sbjct: 421 VGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQANNAYIFPG G+G+VISGAIRVHD+MLLAASEALA QV+ EN++KGLIYPP
Sbjct: 481 NGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           F+NIRKISAHIAANVAAKAYELGLATRLP+P +L+KYAESCMYSPVYR+YR
Sbjct: 541 FSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSYR 591

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BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match: P34105 (NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3)

HSP 1 Score: 1030.8 bits (2664), Expect = 6.1e-300
Identity = 504/591 (85.28%), Postives = 554/591 (93.74%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKE+ DG SVLD+DPKSTVGGGVEDVYGED ATE+QL+TPWT SVASGY+LLRDPH
Sbjct: 1   MESTLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+ ++QQLQEKKLM  IRQYQLPLQK+ AMMEL+ERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIF+RPQGLYISLKEKGK+L+VLKNWPQ+
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQK 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGGVRPSACLP+TIDVGTNNE+LL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
            DEFYIGL+QRRATG+EY ELLHEFMTAVKQNYGEKVLIQFEDFANHNAF+LLAKY T+H
Sbjct: 241 KDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTA+VVLAG +SALKL+GG+LADHTFLFLGAGEAGTGIAELIALE+S+++  
Sbjct: 301 LVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKT 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWL DSKGLIV SRK+SLQHFKKPWAHEHEPV+ LL  VKAIKP VLIG+SG
Sbjct: 361 PLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VG+TFTKEV+EA++S NEKPLI+ALSNPTSQSECTA+EAY W++G+AIFASGSPFDPVEY
Sbjct: 421 VGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
            GK FVPGQ+NNAYIFPG GLG+VISGAIRVHDDMLLAA+EALA Q+  E   KGLIYPP
Sbjct: 481 EGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
            +NIRKIS  IAANVAAKAYELGLATRLPRP +L+K+AESCMYSP YR YR
Sbjct: 541 LSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAYRYYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 3.5e-287
Identity = 479/573 (83.60%), Postives = 532/573 (92.84%), Query Frame = 0 

Query: 19  PKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLR 78
           P + VGGGVEDVYGED ATE+  ITPW+ SVASGYSLLRDPHHNKGLAFTEKERDAHYLR
Sbjct: 75  PTAVVGGGVEDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLR 134

Query: 79  GLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVV 138
           GLLPP +V   LQ KK+M NIRQY++PLQ++ AMM+LQERNERLFYKLLI+N+EELLP+V
Sbjct: 135 GLLPPVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIV 194

Query: 139 YTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGD 198
           YTPTVGEACQKYG+IF+ PQGLYISLK+KGK+LE+LKNWPQ+ IQVIVVTDGERILGLGD
Sbjct: 195 YTPTVGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGD 254

Query: 199 LGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEEY 258
           LGCQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNEK+LNDEFYIGL+QRRA+G+EY
Sbjct: 255 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEY 314

Query: 259 YELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGA 318
            EL++EFM+AVKQNYGEKVLIQFEDFANHNAF+LL KYRT+HLVFNDDIQGTASVVLAG 
Sbjct: 315 AELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGL 374

Query: 319 VSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLVDSKGLI 378
           +SALKL+GG+LADH FLFLGAGEAGTGIAELIALEISK+TN P+EETRKKIWLVDSKGLI
Sbjct: 375 ISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLI 434

Query: 379 VHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINE 438
           V SR DSLQHFKKPWAH+HEPV   L AVKAIKPTVLIGSSG G+TFTKEVVEA+SS NE
Sbjct: 435 VRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNE 494

Query: 439 KPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFPG 498
           KP+I+ALSNPTSQSECTAE+AY WSEGR IFASGSPF PVEYNGK +V GQ+NNAYIFPG
Sbjct: 495 KPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPG 554

Query: 499 FGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAANVAAK 558
           FGLG++ISGAIRVHD+MLLAASEALA QV+ E+++ GLIYPPFTNIRKISAHIAA VAAK
Sbjct: 555 FGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAK 614

Query: 559 AYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           AYELGLA+RLP+P +L+ YAESCMYSP YR YR
Sbjct: 615 AYELGLASRLPQPENLVAYAESCMYSPKYRNYR 647

[Close]
BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 3.2e-285
Identity = 483/582 (82.99%), Postives = 535/582 (91.92%), Query Frame = 0 

Query: 10  DGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTE 69
           + G  + +    + GGGV D+YGED ATE+ LITPWTFSVASG SLLRDP +NKGLAFTE
Sbjct: 8   ENGGEVSVKKDYSNGGGVRDLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTE 67

Query: 70  KERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLID 129
            ERDAHYLRGLLPPS+  Q+LQEK+LM N+RQY++PL +++A+M+LQERNERLFYKLLID
Sbjct: 68  GERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLFYKLLID 127

Query: 130 NVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTD 189
           NV ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWP++SIQVIVVTD
Sbjct: 128 NVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTD 187

Query: 190 GERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLK 249
           GERILGLGDLGCQGMGIPVGKLSLYTALGGVRPS+CLP+TIDVGTNNEKLLNDEFYIGL+
Sbjct: 188 GERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLR 247

Query: 250 QRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQG 309
           QRRATG+EY   L EFM AVKQNYGEKVL+QFEDFANHNAF+LL KY +SHLVFNDDIQG
Sbjct: 248 QRRATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVFNDDIQG 307

Query: 310 TASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKI 369
           TASVVLAG +++LKL+GGTLADHTFLFLGAGEAGTGIAELIA+E+SK+T  PVEETRKKI
Sbjct: 308 TASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKI 367

Query: 370 WLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEV 429
           WLVDSKGLIV SR +SLQ FKKPWAHEHEPV+ LL AVKAIKPTVLIGSSG G+TFTKEV
Sbjct: 368 WLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEV 427

Query: 430 VEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQ 489
           VE ++S+NEKPLI+ALSNPTSQSECTAEEAY WS+GRAIFASGSPFDPVEY GK FVPGQ
Sbjct: 428 VETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQ 487

Query: 490 ANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISA 549
           ANNAYIFPGFGLG+++SGAIRV D+MLLAASEALAAQVS ENY+KGLIYPPFTNIRKISA
Sbjct: 488 ANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISA 547

Query: 550 HIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           +IAA VAAKAY+LGLA+ L RP DL+KYAESCMYSP YR+YR
Sbjct: 548 NIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSYR 589

[Close]
BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match: Q9CA83 (NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NADP-ME4 PE=1 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 2.0e-282
Identity = 468/579 (80.83%), Postives = 537/579 (92.75%), Query Frame = 0 

Query: 13  SVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKER 72
           S  D+ PKSTV GGV+DVYGED ATE+  ITPW+ SVASGY+LLRDPHHNKGLAF+ +ER
Sbjct: 68  SAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRER 127

Query: 73  DAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVE 132
           DAHYLRGLLPP++++Q LQ KK+M  +RQYQ+PLQK++AMM+LQE NERLFYKLLID+VE
Sbjct: 128 DAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVE 187

Query: 133 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGER 192
           ELLPV+YTPTVGEACQKYGSIF RPQGL+ISLKEKGKI EVL+NWP+++IQVIVVTDGER
Sbjct: 188 ELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGER 247

Query: 193 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRR 252
           ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLP+TIDVGTNNEKLLNDEFYIGL+QRR
Sbjct: 248 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRR 307

Query: 253 ATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTAS 312
           ATGEEY EL+HEFMTAVKQNYGEKV+IQFEDFANHNAF+LLAKY T+HLVFNDDIQGTAS
Sbjct: 308 ATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTAS 367

Query: 313 VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLV 372
           VVLAG ++AL+ +GG+L+DH FLFLGAGEAGTGIAELIALEISKK+++P+EE RK IWLV
Sbjct: 368 VVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLV 427

Query: 373 DSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEA 432
           DSKGLIV SRK+S+QHFKKPWAH+HEP+++L+ AVKAIKPTVLIG+SGVG+TFT++VVE 
Sbjct: 428 DSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVET 487

Query: 433 VSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANN 492
           ++ +NEKP+I++LSNPTSQSECTAEEAY WS+GRAIFASGSPF PVEY GKTFVPGQANN
Sbjct: 488 MAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANN 547

Query: 493 AYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIA 552
           AYIFPGFGLG+++SG IRVHDDMLLAASEALA ++  E+YEKG+IYPPF NIRKISA IA
Sbjct: 548 AYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIA 607

Query: 553 ANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           A VAAKAYELGLATRLP+P +L + AES MYSP YR+YR
Sbjct: 608 AKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSYR 646

[Close]
BLAST of MC06g0719 vs. NCBI nr
Match: XP_022144991.1 (NADP-dependent malic enzyme [Momordica charantia])

HSP 1 Score: 1170 bits (3028), Expect = 0.0
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP
Sbjct: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591

[Close]
BLAST of MC06g0719 vs. NCBI nr
Match: KAG6589949.1 (hypothetical protein SDJN03_15372, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 573/591 (96.95%), Postives = 585/591 (98.98%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK+TN 
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYEKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. NCBI nr
Match: XP_023516304.1 (NADP-dependent malic enzyme [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1144 bits (2958), Expect = 0.0
Identity = 572/591 (96.79%), Postives = 585/591 (98.98%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIV+QQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVSQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK+TN 
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYEKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. NCBI nr
Match: XP_022961041.1 (NADP-dependent malic enzyme [Cucurbita moschata])

HSP 1 Score: 1143 bits (2957), Expect = 0.0
Identity = 572/591 (96.79%), Postives = 584/591 (98.82%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK+TN 
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYE GLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYENGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. NCBI nr
Match: XP_022987661.1 (NADP-dependent malic enzyme [Cucurbita maxima])

HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 571/591 (96.62%), Postives = 584/591 (98.82%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SK+T  
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYEKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match: A0A6J1CV17 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111014522 PE=3 SV=1)

HSP 1 Score: 1170 bits (3028), Expect = 0.0
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP
Sbjct: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match: A0A6J1H992 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111461666 PE=3 SV=1)

HSP 1 Score: 1143 bits (2957), Expect = 0.0
Identity = 572/591 (96.79%), Postives = 584/591 (98.82%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK+TN 
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYE GLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYENGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match: A0A6J1JEY0 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111485147 PE=3 SV=1)

HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 571/591 (96.62%), Postives = 584/591 (98.82%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SK+T  
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS ENYEKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAA VAAKAYELGLATRLPRPADL+KYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match: A0A0A0LW91 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1)

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 571/591 (96.62%), Postives = 585/591 (98.98%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLL+DNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLVDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SK+T  
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVS ENY+KGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAANVAAKAYELGLATRLPRPADL+KYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match: A0A5D3CG29 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 PE=3 SV=1)

HSP 1 Score: 1140 bits (2949), Expect = 0.0
Identity = 571/591 (96.62%), Postives = 585/591 (98.98%), Query Frame = 0 

Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVED+YGEDCATEEQL+TPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK+T  
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRKDSLQHFK+PWAHEHEPV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKQPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480
           VGRTFTKEV+EAVSSINEKPLIMALSNPTSQSECTAEEAY WSEGRAIFASGSPFDP EY
Sbjct: 421 VGRTFTKEVIEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVS ENY+KGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 591
           FTNIRKISA+IAANVAAKAYELGLATRLPRPADL+KYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]
BLAST of MC06g0719 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 972.6 bits (2513), Expect = 1.4e-283
Identity = 468/579 (80.83%), Postives = 537/579 (92.75%), Query Frame = 0 

Query: 13  SVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKER 72
           S  D+ PKSTV GGV+DVYGED ATE+  ITPW+ SVASGY+LLRDPHHNKGLAF+ +ER
Sbjct: 68  SAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRER 127

Query: 73  DAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVE 132
           DAHYLRGLLPP++++Q LQ KK+M  +RQYQ+PLQK++AMM+LQE NERLFYKLLID+VE
Sbjct: 128 DAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVE 187

Query: 133 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGER 192
           ELLPV+YTPTVGEACQKYGSIF RPQGL+ISLKEKGKI EVL+NWP+++IQVIVVTDGER
Sbjct: 188 ELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGER 247

Query: 193 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRR 252
           ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLP+TIDVGTNNEKLLNDEFYIGL+QRR
Sbjct: 248 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRR 307

Query: 253 ATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTAS 312
           ATGEEY EL+HEFMTAVKQNYGEKV+IQFEDFANHNAF+LLAKY T+HLVFNDDIQGTAS
Sbjct: 308 ATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTAS 367

Query: 313 VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLV 372
           VVLAG ++AL+ +GG+L+DH FLFLGAGEAGTGIAELIALEISKK+++P+EE RK IWLV
Sbjct: 368 VVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLV 427

Query: 373 DSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEA 432
           DSKGLIV SRK+S+QHFKKPWAH+HEP+++L+ AVKAIKPTVLIG+SGVG+TFT++VVE 
Sbjct: 428 DSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVET 487

Query: 433 VSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANN 492
           ++ +NEKP+I++LSNPTSQSECTAEEAY WS+GRAIFASGSPF PVEY GKTFVPGQANN
Sbjct: 488 MAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANN 547

Query: 493 AYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIA 552
           AYIFPGFGLG+++SG IRVHDDMLLAASEALA ++  E+YEKG+IYPPF NIRKISA IA
Sbjct: 548 AYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIA 607

Query: 553 ANVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           A VAAKAYELGLATRLP+P +L + AES MYSP YR+YR
Sbjct: 608 AKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSYR 646

[Close]
BLAST of MC06g0719 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 966.5 bits (2497), Expect = 1.0e-281
Identity = 481/578 (83.22%), Postives = 530/578 (91.70%), Query Frame = 0 

Query: 14  VLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKERD 73
           V + D KS+V GGV DVYGED AT E  ITPW+ SV+SGYSLLRDP +NKGLAFTEKERD
Sbjct: 4   VTNSDLKSSVDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERD 63

Query: 74  AHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEE 133
            HYLRGLLPP ++ Q+LQEK+L+ NIRQYQ PLQK++A+ ELQERNERLFYKLLIDNVEE
Sbjct: 64  THYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEE 123

Query: 134 LLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERI 193
           LLP+VYTPTVGEACQK+GSIFRRPQGL+ISLK+KGKIL+VLKNWP+R+IQVIVVTDGERI
Sbjct: 124 LLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERI 183

Query: 194 LGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRA 253
           LGLGDLGCQGMGIPVGKL+LY+ALGGVRPSACLP+TIDVGTNNEKLLNDEFYIGL+Q+RA
Sbjct: 184 LGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRA 243

Query: 254 TGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASV 313
           TG+EY ELL+EFM+AVKQNYGEKVLIQFEDFANHNAFELLAKY  +HLVFNDDIQGTASV
Sbjct: 244 TGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASV 303

Query: 314 VLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLVD 373
           VLAG VSA KL    LA+HTFLFLGAGEAGTGIAELIAL +SK+ N  VEE+RKKIWLVD
Sbjct: 304 VLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVD 363

Query: 374 SKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAV 433
           SKGLIV+SRKDSLQ FKKPWAHEHEPV+DLL A+KAIKPTVLIGSSGVGR+FTKEV+EA+
Sbjct: 364 SKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM 423

Query: 434 SSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANNA 493
           SSINE+PLIMALSNPT+QSECTAEEAY WS+GRAIFASGSPFDPVEY GK FV  QANNA
Sbjct: 424 SSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNA 483

Query: 494 YIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAA 553
           YIFPGFGLG+VISGAIRVHDDMLLAA+EALA QVS ENYEKG+IYP F++IRKISA IAA
Sbjct: 484 YIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAA 543

Query: 554 NVAAKAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           NVA KAYELGLA RLPRP D++K AES MYSP YR YR
Sbjct: 544 NVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYRLYR 581

[Close]
BLAST of MC06g0719 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-277
Identity = 464/574 (80.84%), Postives = 527/574 (91.81%), Query Frame = 0 

Query: 18  DPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYL 77
           D +S VGGG+ DVYGED AT +QL+TPW  SVASGY+L+RDP +NKGLAFT+KERDAHYL
Sbjct: 15  DNRSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYL 74

Query: 78  RGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPV 137
            GLLPP I++Q +QE+K+M N+RQY +PLQ+++A+M+LQERNERLFYKLLIDNVEELLPV
Sbjct: 75  TGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPV 134

Query: 138 VYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLG 197
           VYTPTVGEACQKYGSIFR+PQGLYISL EKGKILEVLKNWPQR IQVIVVTDGERILGLG
Sbjct: 135 VYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLG 194

Query: 198 DLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEE 257
           DLGCQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATG+E
Sbjct: 195 DLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQE 254

Query: 258 YYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAG 317
           Y E LHEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY  SHLVFNDDIQGTASVVLAG
Sbjct: 255 YAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAG 314

Query: 318 AVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLVDSKGL 377
            ++A K++G  LADHTFLFLGAGEAGTGIAELIAL+ISK+T  P+ ETRKKIWLVDSKGL
Sbjct: 315 LIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGL 374

Query: 378 IVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSIN 437
           IV SRK+SLQHFK+PWAHEH+PV+DL+ AV AIKPTVLIG+SGVG+TFTKEVVEA+++ N
Sbjct: 375 IVSSRKESLQHFKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNN 434

Query: 438 EKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFP 497
           EKPLI+ALSNPTSQ+ECTAE+AY W++GRAIF SGSPFDPV Y+GKT++PGQANN YIFP
Sbjct: 435 EKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFP 494

Query: 498 GFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAANVAA 557
           G GLG+++SGAIRV DDMLLAASEALAAQV+ E+Y  GLIYPPF+NIR+ISA+IAA VAA
Sbjct: 495 GLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAA 554

Query: 558 KAYELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           K Y+LGLA+ LPR  DL+K+AES MYSPVYR YR
Sbjct: 555 KTYDLGLASNLPRAKDLVKFAESSMYSPVYRNYR 588

[Close]
BLAST of MC06g0719 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-277
Identity = 460/571 (80.56%), Postives = 528/571 (92.47%), Query Frame = 0 

Query: 21  STVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGL 80
           S VGGG+ DVYGED AT +QL+TPW  SVASGY+L+RDP +NKGLAFT+KERDAHY+ GL
Sbjct: 18  SGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGL 77

Query: 81  LPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYT 140
           LPP +++Q +QE+K+M N+RQY +PLQ+++A+M+LQERNERLFYKLLIDNVEELLPVVYT
Sbjct: 78  LPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYT 137

Query: 141 PTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLG 200
           PTVGEACQKYGSI+RRPQGLYISLKEKGKILEVLKNWPQR IQVIVVTDGERILGLGDLG
Sbjct: 138 PTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLG 197

Query: 201 CQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEEYYE 260
           CQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNEKLLN+EFYIGLKQ+RA GEEY E
Sbjct: 198 CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAE 257

Query: 261 LLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVS 320
            L EFM AVKQNYGEKVL+QFEDFANH+AFELL+KY +SHLVFNDDIQGTASVVLAG ++
Sbjct: 258 FLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIA 317

Query: 321 ALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNVPVEETRKKIWLVDSKGLIVH 380
           A K++G +LADHTFLFLGAGEAGTGIAELIAL+ISK+T  P++ETRKKIWLVDSKGLIV 
Sbjct: 318 AQKVLGKSLADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVS 377

Query: 381 SRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINEKP 440
            RK+SLQHFK+PWAH+H+PV++LL+AV AIKPTVLIG+SGVG+TFTKEVVEA++++NEKP
Sbjct: 378 ERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKP 437

Query: 441 LIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFPGFG 500
           LI+ALSNPTSQ+ECTAEEAY W++GRAIFASGSPFDPV+Y+GK F PGQANN YIFPG G
Sbjct: 438 LILALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLG 497

Query: 501 LGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAANVAAKAY 560
           LG+++SGAIRV DDMLLAASEALA+QV+ EN+  GLIYPPF NIRKISA+IAA+V AK Y
Sbjct: 498 LGLIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTY 557

Query: 561 ELGLATRLPRPADLMKYAESCMYSPVYRTYR 592
           ELGLA+ LPRP DL+K AESCMYSPVYR +R
Sbjct: 558 ELGLASNLPRPKDLVKMAESCMYSPVYRNFR 588

[Close]
BLAST of MC06g0719 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.5e-120
Identity = 242/574 (42.16%), Postives = 351/574 (61.15%), Query Frame = 0 

Query: 52  GYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQ-------- 111
           G  +L DP  NKG AFT  ER+   LRGLLPP+++  + Q  + M ++++ +        
Sbjct: 46  GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPS 105

Query: 112 --LPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLY 171
               L K+  +  L +RNE ++YK+LI+N+EE  P+VYTPTVG  CQ Y  +FRRP+G+Y
Sbjct: 106 DPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMY 165

Query: 172 ISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVR 231
            S +++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI VGKL LY A  G+ 
Sbjct: 166 FSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGIN 225

Query: 232 PSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQF 291
           P   LP+ IDVGTNNEKL ND  Y+GL+QRR   ++Y +++ EFM AV   +   V++QF
Sbjct: 226 PQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-PHVIVQF 285

Query: 292 EDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVSALKLIGGTLADH---TFLFLG 351
           EDF +  AF+LL +YR ++ +FNDD+QGTA V +AG + A++  G  + D      +  G
Sbjct: 286 EDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAG 345

Query: 352 AGEAGTGIAELIALEISK---KTNVPVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAH 411
           AG AG G+       +++    T    +  + + W+VD++GLI   R +++    +P+A 
Sbjct: 346 AGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPEAQPFAR 405

Query: 412 EHEPVQ--------DLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAV-SSINEKPLIMALS 471
           + + ++         L+  V+ +KP VL+G S VG  F+KEV+EA+  S + +P I A+S
Sbjct: 406 KTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMS 465

Query: 472 NPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY-NGKTFVPGQANNAYIFPGFGLGVVI 531
           NPT  +ECT ++A++      IFASGSPF  VE+ NG      Q NN Y+FPG GLG ++
Sbjct: 466 NPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLL 525

Query: 532 SGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPPFTNIRKISAHIAANVAAKAYELGLA 590
           SGA  V D ML AASE LAA +S E   +G+IYPP + IR I+  IAA V  +A E  L 
Sbjct: 526 SGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLV 585

The following BLAST results are available for this feature:
BLAST of MC06g0719 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P516157.7e-30386.97NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
P341056.1e-30085.28NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3[more]
P364443.5e-28783.60NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ... [more]
P126283.2e-28582.99NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1[more]
Q9CA832.0e-28280.83NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
BLAST of MC06g0719 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022144991.10.0100.00NADP-dependent malic enzyme [Momordica charantia][more]
KAG6589949.10.096.95hypothetical protein SDJN03_15372, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023516304.10.096.79NADP-dependent malic enzyme [Cucurbita pepo subsp. pepo][more]
XP_022961041.10.096.79NADP-dependent malic enzyme [Cucurbita moschata][more]
XP_022987661.10.096.62NADP-dependent malic enzyme [Cucurbita maxima][more]
BLAST of MC06g0719 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CV170.0100.00Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111014522 PE=3 SV=1[more]
A0A6J1H9920.096.79Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111461666 PE=3 SV=1[more]
A0A6J1JEY00.096.62Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111485147 PE=3 SV=1[more]
A0A0A0LW910.096.62Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1[more]
A0A5D3CG290.096.62Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 P... [more]
BLAST of MC06g0719 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G79750.11.4e-28380.83NADP-malic enzyme 4 [more]
AT2G19900.11.0e-28183.22NADP-malic enzyme 1 [more]
AT5G11670.11.1e-27780.84NADP-malic enzyme 2 [more]
AT5G25880.11.5e-27780.56NADP-malic enzyme 3 [more]
AT2G13560.11.5e-12042.16NAD-dependent malic enzyme 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 122..146
score: 69.8
coord: 278..296
score: 71.18
coord: 182..211
score: 82.75
coord: 334..350
score: 71.32
coord: 437..453
score: 66.62
coord: 218..240
score: 69.35
coord: 303..319
score: 74.71
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 27..591
e-value: 4.8E-237
score: 785.2
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 307..560
e-value: 1.9E-112
score: 389.5
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 307..559
e-value: 1.7E-95
score: 319.2
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 116..297
e-value: 1.4E-103
score: 360.0
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 116..297
e-value: 2.0E-79
score: 265.7
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 50..304
e-value: 5.9E-126
score: 420.8
NoneNo IPR availableGENE3D3.40.50.720coord: 305..591
e-value: 4.8E-121
score: 405.8
NoneNo IPR availablePIRSRPIRSR000106-3PIRSR000106-3coord: 54..390
e-value: 1.5E-140
score: 466.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 20..591
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 20..591
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 307..585
e-value: 1.3251E-145
score: 420.031
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 31..306
IPR015884Malic enzyme, conserved sitePROSITEPS00331MALIC_ENZYMEScoord: 303..319
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 307..590

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0719.1MC06g0719.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity