MC06g0683 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0683 vs. ExPASy Swiss-Prot
Match: A5ERG8 (tRNA(Ile)-lysidine synthase OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) OX=288000 GN=tilS PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.0e-21
Identity = 72/199 (36.18%), Postives = 100/199 (50.25%), Query Frame = 0

Query: 83  IALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAESRDEAKVVW 142
           + L VSGGPDSMAL  L A W+     A G R      LLA+ VDHGLR ESR EA +V 
Sbjct: 30  LTLAVSGGPDSMALMWLAARWR--ATRAGGPR------LLAVTVDHGLRPESRREALMVK 89

Query: 143 NRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVILVAHHADDQ 202
                LG+     +  WS  +P  G + EAAR  RY++L +   Q     ++ AH  DDQ
Sbjct: 90  QLARQLGLTHR--TLRWSGEKPAAG-IPEAARIARYRLLARAAEQAGASHVVTAHTRDDQ 149

Query: 203 IFS-----------SHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTN 262
             +           +   + + VS+  G+LL RPL+  SK  +    + A   + +DPTN
Sbjct: 150 AETVLMRLLRGSGIAGLAAMAPVSRRDGLLLARPLLSLSKARLLATLRSAGIAFADDPTN 209

Query: 263 RSSLFARNRIRMSLGDLSS 271
           R   F R R+R  +  L++
Sbjct: 210 RDPAFTRPRLRALMPTLAA 217

BLAST of MC06g0683 vs. ExPASy Swiss-Prot
Match: Q9XBG6 (tRNA(Ile)-lysidine synthase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=tilS PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.0e-20
Identity = 71/207 (34.30%), Postives = 102/207 (49.28%), Query Frame = 0

Query: 75  AGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAES 134
           A LK    + L VSGGPDS+AL  L A W+     A G R      L  + VDHGLRAE+
Sbjct: 19  AELKSAPALVLAVSGGPDSIALMWLAARWQRS--LARGPR------LTVVTVDHGLRAEA 78

Query: 135 RDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVIL 194
             EA+ V    T LG+     +  W   +PK G L  AAR+ RY++L +         +L
Sbjct: 79  AREAREVKRLATELGL--PHRTLRWRGAKPKTG-LPAAAREARYRLLMQAARSAGASHVL 138

Query: 195 VAHHADDQIFS-----------SHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQ 254
            AH  DDQ  +           +   + + +++  GI+L RPL+D  K  +    + A  
Sbjct: 139 TAHTRDDQAETLLMRLVRGSGLAGLSAMARLTERDGIVLARPLLDVPKAQLIATLKRAKI 198

Query: 255 DWVEDPTNRSSLFARNRIRMSLGDLSS 271
            + +DPTNR + F R R+R  L  L++
Sbjct: 199 GFADDPTNRDTAFTRPRLRALLPQLAA 214

BLAST of MC06g0683 vs. ExPASy Swiss-Prot
Match: B3CLY6 (tRNA(Ile)-lysidine synthase OS=Wolbachia pipientis subsp. Culex pipiens (strain wPip) OX=570417 GN=tilS PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.4e-18
Identity = 89/320 (27.81%), Postives = 145/320 (45.31%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAESRDEAK 139
           H++IA+ VSGG DS+ L  L  +W          + N +   +A+ V+HGLR+ES+ EA 
Sbjct: 17  HNQIAVAVSGGVDSIVLLHLMTNW---------AKKNKLSLPIALTVNHGLRSESQKEAD 76

Query: 140 VVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVILVAHHA 199
            + +    LG + E    +W     K G++Q  AR  RY++L + C    +  +LVAHH 
Sbjct: 77  FIISYAKELGAK-ESFILNWEKQNIK-GNIQLQARKARYKLLAEWCKNNNVKCLLVAHHK 136

Query: 200 DDQI---------------FSSHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQD 259
           DDQ                 SS  Y     S   GI + RPL++FS+ ++ +  +     
Sbjct: 137 DDQAETFLLRLERGSGVDGLSSMDYK----SFLNGIYIFRPLLNFSRSEIERYAKLHQLR 196

Query: 260 WVEDPTN-----RSSLFARNRIRMS-----LGDLSSCTLKSELRAIISACRKTRLFIDQV 319
           W+ED +N     R +L+ RN ++ S     L +    T     RA  +    TRL  D  
Sbjct: 197 WIEDRSNYDLKYRRTLY-RNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFD-- 256

Query: 320 CLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPVRGNTAKLV 375
                   + + + GY  I L    QL  ++I L   +  ++   S+ ++P R N+  ++
Sbjct: 257 ------DCVNVHDFGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP-RYNSLIVI 310

BLAST of MC06g0683 vs. ExPASy Swiss-Prot
Match: C0R4S1 (tRNA(Ile)-lysidine synthase OS=Wolbachia sp. subsp. Drosophila simulans (strain wRi) OX=66084 GN=tilS PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.4e-18
Identity = 86/313 (27.48%), Postives = 143/313 (45.69%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAESRDEAK 139
           ++++A+ VSGG DS+ L  L  +W          + N +    A+ V+HGLR ES+ EA 
Sbjct: 18  NNQVAVAVSGGVDSIVLLHLMTNW---------AKKNKLSLPTALTVNHGLRPESQKEAD 77

Query: 140 VVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVILVAHHA 199
            V +    LG + E    +W     K G++Q  AR  RY++L + C    +  +LVAHH 
Sbjct: 78  FVVSYAKELGAK-ESFILNWEKQNIK-GNIQLQARKARYKLLAEWCKNNNVKYLLVAHHK 137

Query: 200 DDQI---------------FSSHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQD 259
           DDQ                 SS  Y     S   GI + RPL++FS+ ++ K  +     
Sbjct: 138 DDQAETFLLRLERGSGVDGLSSMDYK----SFLNGIYIFRPLLNFSRSEIEKYAKLHRLK 197

Query: 260 WVEDPTNRSSLFARNRIRMSLGDLSSCTLKSELRAIISAC---RKTRLFIDQVCLSLANQ 319
           W+ED +N    + R   R  L    +  + +E R  ++A    R  +  +    L+  N 
Sbjct: 198 WIEDRSNYDLKYRRTLYRNLLKASDNQEILTE-RICLTALHMKRAAKALMHYTRLAF-ND 257

Query: 320 TLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPVRGNTAKLVLDYIRTV 375
            + + +LGY  I L    QL  ++I L   +  ++   S+ ++P R N+  ++ + I   
Sbjct: 258 CVNVHDLGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP-RYNSLIVIFNKILQK 311

BLAST of MC06g0683 vs. ExPASy Swiss-Prot
Match: Q73FR9 (tRNA(Ile)-lysidine synthase OS=Wolbachia pipientis wMel OX=163164 GN=tilS PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 9.1e-18
Identity = 85/313 (27.16%), Postives = 143/313 (45.69%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAESRDEAK 139
           ++++A+ VSGG DS+ L  L  +W          + N +   +A+ V+HGLR ES+ EA 
Sbjct: 18  NNQVAVAVSGGVDSIVLLHLMTNW---------AKKNKLSLPIALTVNHGLRPESQKEAD 77

Query: 140 VVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVILVAHHA 199
            V +    LG + E    +W     K G++Q  AR  RY++L + C    +  +LVAHH 
Sbjct: 78  FVVSYAKELGAK-ESFILNWEKQNIK-GNIQLQARKARYKLLAEWCKNNNVKYLLVAHHK 137

Query: 200 DDQI---------------FSSHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQD 259
           DDQ                 SS  Y     S   GI + RPL++FS+ ++ K  +     
Sbjct: 138 DDQAETFLLRLERGSGVDGLSSMDYK----SFLNGIDIFRPLLNFSRSEIEKYAKLHRLK 197

Query: 260 WVEDPTNRSSLFARNRIRMSLGDLSSCTLKSELRAIISAC---RKTRLFIDQVCLSLANQ 319
           W+ED +N    + R   R  L    +  + +E R  ++A    R  +  +    L+  N 
Sbjct: 198 WIEDRSNHDLKYRRTLYRNLLKASDNQEILTE-RICLTALHMKRAAKALMHYTRLAF-ND 257

Query: 320 TLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPVRGNTAKLVLDYIRTV 375
            + + +LGY  I L    QL  ++I L   +  ++   S+ ++P R ++   + + I   
Sbjct: 258 CVNVHDLGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP-RYSSLIAIFNRILQK 311

BLAST of MC06g0683 vs. NCBI nr
Match: XP_022145007.1 (uncharacterized protein LOC111014535 isoform X1 [Momordica charantia])

HSP 1 Score: 1331 bits (3444), Expect = 0.0
Identity = 667/693 (96.25%), Postives = 667/693 (96.25%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD
Sbjct: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG
Sbjct: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQQKIGVILVAHHADDQ                         IFSSHTYSYSEVS
Sbjct: 181 LQKVCVQQKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSSHTYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS
Sbjct: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV
Sbjct: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL
Sbjct: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR
Sbjct: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN
Sbjct: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD
Sbjct: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL
Sbjct: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSIRFSHCPWLMASAEFK RVPFGGGHSSFM
Sbjct: 661 SIPSIRFSHCPWLMASAEFKLRVPFGGGHSSFM 693

BLAST of MC06g0683 vs. NCBI nr
Match: KAG6587963.1 (hypothetical protein SDJN03_16528, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 571/693 (82.40%), Postives = 620/693 (89.47%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MA+ LIP  Q+ T+HSFF+ S+KSKF SPR +F F LPRLRLLPLSRCFCR+SS+QEP+D
Sbjct: 1   MARSLIPRAQSGTSHSFFILSSKSKFSSPRFQFSFMLPRLRLLPLSRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           +C YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WKMD LNATGK S FIDG
Sbjct: 61  MCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKMDSLNATGKSSTFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQ KIGVILVAHHADDQ                         IFS H Y YSEVS
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYFYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KN GILLVRPLMDFSKDD+YKICQGANQDWVEDPTNRSSLFARNRIR+SLGD+SSCTLKS
Sbjct: 241 KNYGILLVRPLMDFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRISLGDMSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           EL AIISACRKTRLF+D  CLSLAN TLTITELGYAIINLEILNQLKI+DICLSKF+SLV
Sbjct: 301 ELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNQLKIQDICLSKFLSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRG+TAKLVLDYI+TVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPL
Sbjct: 361 LQFISQRHRPVRGSTAKLVLDYIQTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSA+EVFH PSY+EE+HDIS GLEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKR
Sbjct: 421 PSAVEVFHVPSYKEERHDISGGLEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNI+ ESTYRII+SLQMQETKLFKA+ K TPGYESKHGSHSV TLSSGLLKSGQ+CN+M+
Sbjct: 481 LNILGESTYRIIVSLQMQETKLFKAEPKVTPGYESKHGSHSVSTLSSGLLKSGQICNYMS 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RF ITWK++DENEDNSI +AASCNT+CK+C LGCDMELEVRHMTESDWLYLSKLSECTV+
Sbjct: 541 RFFITWKVKDENEDNSISDAASCNTICKSCALGCDMELEVRHMTESDWLYLSKLSECTVE 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           +F DQRDTS+S + QTME+ + CL YA+SSAR+ALVLLKLIPVAAR+SLPVLVN +GLLL
Sbjct: 601 NFHDQRDTSASSKEQTMENASRCLKYASSSARKALVLLKLIPVAARKSLPVLVNQKGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSI+FSHCPWLMAS EFKPRVPFGGGHSSFM
Sbjct: 661 SIPSIKFSHCPWLMASVEFKPRVPFGGGHSSFM 693

BLAST of MC06g0683 vs. NCBI nr
Match: XP_023529428.1 (uncharacterized protein LOC111792288 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 574/693 (82.83%), Postives = 619/693 (89.32%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MAQ LIP  Q+ T+HSFFL S+KSKF SPR +FPF LPRLRLLPL RCFCR+SS+QEP+D
Sbjct: 1   MAQSLIPRAQSGTSHSFFLLSSKSKFSSPRFQFPFMLPRLRLLPLCRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           LC YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WKMD LNATGK S FIDG
Sbjct: 61  LCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKMDSLNATGKSSTFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQ KIGVILVAHHADDQ                         IFS H YSYSEVS
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KN GILLVRPLMDFSKDD+YKICQGANQDWVEDPTNRSSLFARNRIRMSLGD+SSCTLKS
Sbjct: 241 KNYGILLVRPLMDFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           EL AIISACRKTRLF+D  CLSLAN TLTITELGYAIINLEILNQLKI+DICLSKF+SLV
Sbjct: 301 ELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNQLKIQDICLSKFLSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRG+TAKLVLDYI+TVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPL
Sbjct: 361 LQFISQRHRPVRGSTAKLVLDYIQTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSA+EVFH PSY+EE+HDIS GLEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKR
Sbjct: 421 PSAVEVFHVPSYKEERHDISGGLEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNI+SESTYRII+SLQ+QETKLFK++ K T GYESKHGSHSV TLSSGLLKSGQ+CN+MN
Sbjct: 481 LNILSESTYRIIVSLQIQETKLFKSEPKITSGYESKHGSHSVSTLSSGLLKSGQICNYMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RF ITWK++D NEDNSI +AASCNT+ K+C LGCDMELEVRHMTESDWLYLSKLSECTV 
Sbjct: 541 RFFITWKVKDANEDNSISDAASCNTIYKSCALGCDMELEVRHMTESDWLYLSKLSECTVK 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           +F DQRDTS+S + QTME+ + CL YA+SSAR+ALVLLKLIPVAAR+SLPVLVN +GLLL
Sbjct: 601 NFHDQRDTSASSKEQTMENASRCLKYASSSARKALVLLKLIPVAARKSLPVLVNQKGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSI+FSHCPWLMASAEFKPRVPFGGGHSSFM
Sbjct: 661 SIPSIKFSHCPWLMASAEFKPRVPFGGGHSSFM 693

BLAST of MC06g0683 vs. NCBI nr
Match: KAG7021853.1 (tilS, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1155 bits (2987), Expect = 0.0
Identity = 570/671 (84.95%), Postives = 619/671 (92.25%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MA+ LIP  Q+ T+HSFF+ S+KSKF SPR  F F LPRLRLLPLSRCFCR+SS+QEP+D
Sbjct: 1   MARSLIPRAQSGTSHSFFVLSSKSKFSSPRFLFSFMLPRLRLLPLSRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           +C YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WKMD LNATGK S FIDG
Sbjct: 61  MCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKMDSLNATGKSSTFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQI--FSSHT-YSYSEVSKNCGILLVRPLMDFSKDDMYKI 240
           LQKVCVQ KIGVILVAHHADDQ+  FS     SYSEVSKN GILLVRPLMDFSKDD+YKI
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQLVPFSPRIDISYSEVSKNYGILLVRPLMDFSKDDLYKI 240

Query: 241 CQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKSELRAIISACRKTRLFIDQVCLS 300
           CQGANQDWVEDPTNRSSLFARNRIR+SLGD+SSCTLKSEL AIISACRKTRLF+D  CLS
Sbjct: 241 CQGANQDWVEDPTNRSSLFARNRIRISLGDMSSCTLKSELHAIISACRKTRLFVDHACLS 300

Query: 301 LANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPVRGNTAKLVLDY 360
           LAN TLTITELGYAIINLEILNQLKI+DICLSKF+SLVLQFISQRHRPVRG+TAKLVLDY
Sbjct: 301 LANLTLTITELGYAIINLEILNQLKIQDICLSKFLSLVLQFISQRHRPVRGSTAKLVLDY 360

Query: 361 IRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPLPSAIEVFHAPSYREEKHDISDG 420
           I+TVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPLPSA+EVFH PSY+EE+HDIS G
Sbjct: 361 IQTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPLPSAVEVFHVPSYKEERHDISGG 420

Query: 421 LEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKRLNIISESTYRIIISLQMQETKL 480
           LEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKRLNI+ ESTYRII+SLQMQETKL
Sbjct: 421 LEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKRLNILGESTYRIIVSLQMQETKL 480

Query: 481 FKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMNRFSITWKIRDENEDNSIFEAAS 540
           FKA+ K TPGYESKHGSHSV TLSSGLLKSGQ+CN+M+RF ITWK++DENEDNSI +AAS
Sbjct: 481 FKAEPKVTPGYESKHGSHSVSTLSSGLLKSGQICNYMSRFFITWKVKDENEDNSISDAAS 540

Query: 541 CNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVDDFQDQRDTSSSLELQTMESPNG 600
           CNT+CK+C LGCDMELEVRHMTESDWLYLSKLSECTV++F DQRDTS+S + QTME+ + 
Sbjct: 541 CNTICKSCALGCDMELEVRHMTESDWLYLSKLSECTVENFHDQRDTSASSKEQTMENASR 600

Query: 601 CLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLLSIPSIRFSHCPWLMASAEFKPR 660
           CL YA+SSAR+ALVLLKLIPVAAR+SLPVLVN +GLLLSIPSI+FSHCPWLMASAEFKPR
Sbjct: 601 CLKYASSSARKALVLLKLIPVAARKSLPVLVNQKGLLLSIPSIKFSHCPWLMASAEFKPR 660

Query: 661 VPFGGGHSSFM 668
           VPFGGGHSSFM
Sbjct: 661 VPFGGGHSSFM 671

BLAST of MC06g0683 vs. NCBI nr
Match: XP_022933762.1 (uncharacterized protein LOC111441079 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 570/693 (82.25%), Postives = 618/693 (89.18%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MA+ LIP  Q+ T+HSFF+ S+KSKF SPR +F F LPRLRLLPLSRCFCR+SS+QEP+D
Sbjct: 1   MARSLIPRAQSGTSHSFFVLSSKSKFSSPRFQFSFMLPRLRLLPLSRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           +C YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WKMD LNATGK S FIDG
Sbjct: 61  MCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKMDSLNATGKSSTFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQ KIGVILVAHHADDQ                         IFS H YSYSEVS
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KN GILLVRPLMDFSKDD+YKICQGANQDWVEDPTNRSSLFARNRIR+SLGD+SSCTLKS
Sbjct: 241 KNYGILLVRPLMDFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRISLGDMSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           EL AIISACRKTRLF+D  CLSLAN TLTITELGYAIINLEILN+LKI+DICLSKF+SLV
Sbjct: 301 ELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNRLKIQDICLSKFLSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRG+TAKLVLDYIRTVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPL
Sbjct: 361 LQFISQRHRPVRGSTAKLVLDYIRTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSA+EVFH PSY+EE+HDIS GLEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKR
Sbjct: 421 PSAVEVFHVPSYKEERHDISGGLEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNI+ ESTYRII+SLQMQETKLFKA+ K TPGY SKHGSHSV TLSSGLLKSGQ+CN+MN
Sbjct: 481 LNILGESTYRIIVSLQMQETKLFKAEPKVTPGYGSKHGSHSVSTLSSGLLKSGQICNYMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RF ITWK++DENEDNSI +AASCNT+CK+C LGCDMELEVRHMTESDWLYLSK SECTV+
Sbjct: 541 RFFITWKVKDENEDNSISDAASCNTICKSCALGCDMELEVRHMTESDWLYLSKFSECTVE 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           +F DQRDTS+S + QTME+ + CL YA+SSAR+ALVLLKLIPVAAR+ LPVLVN +GLLL
Sbjct: 601 NFHDQRDTSASSKEQTMENASRCLKYASSSARKALVLLKLIPVAARKGLPVLVNQKGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSI+FSHCPWLMASAEFKPRVPFGGG SSFM
Sbjct: 661 SIPSIKFSHCPWLMASAEFKPRVPFGGGQSSFM 693

BLAST of MC06g0683 vs. ExPASy TrEMBL
Match: A0A6J1CVA6 (tRNA(Ile)-2-lysyl-cytidine synthase OS=Momordica charantia OX=3673 GN=LOC111014535 PE=3 SV=1)

HSP 1 Score: 1331 bits (3444), Expect = 0.0
Identity = 667/693 (96.25%), Postives = 667/693 (96.25%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD
Sbjct: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG
Sbjct: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQQKIGVILVAHHADDQ                         IFSSHTYSYSEVS
Sbjct: 181 LQKVCVQQKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSSHTYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS
Sbjct: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV
Sbjct: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL
Sbjct: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR
Sbjct: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN
Sbjct: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD
Sbjct: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL
Sbjct: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSIRFSHCPWLMASAEFK RVPFGGGHSSFM
Sbjct: 661 SIPSIRFSHCPWLMASAEFKLRVPFGGGHSSFM 693

BLAST of MC06g0683 vs. ExPASy TrEMBL
Match: A0A6J1F0R0 (tRNA(Ile)-2-lysyl-cytidine synthase OS=Cucurbita moschata OX=3662 GN=LOC111441079 PE=3 SV=1)

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 570/693 (82.25%), Postives = 618/693 (89.18%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MA+ LIP  Q+ T+HSFF+ S+KSKF SPR +F F LPRLRLLPLSRCFCR+SS+QEP+D
Sbjct: 1   MARSLIPRAQSGTSHSFFVLSSKSKFSSPRFQFSFMLPRLRLLPLSRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           +C YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WKMD LNATGK S FIDG
Sbjct: 61  MCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKMDSLNATGKSSTFIDG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQ KIGVILVAHHADDQ                         IFS H YSYSEVS
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KN GILLVRPLMDFSKDD+YKICQGANQDWVEDPTNRSSLFARNRIR+SLGD+SSCTLKS
Sbjct: 241 KNYGILLVRPLMDFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRISLGDMSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           EL AIISACRKTRLF+D  CLSLAN TLTITELGYAIINLEILN+LKI+DICLSKF+SLV
Sbjct: 301 ELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNRLKIQDICLSKFLSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRG+TAKLVLDYIRTVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPL
Sbjct: 361 LQFISQRHRPVRGSTAKLVLDYIRTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSA+EVFH PSY+EE+HDIS GLEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKR
Sbjct: 421 PSAVEVFHVPSYKEERHDISGGLEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNI+ ESTYRII+SLQMQETKLFKA+ K TPGY SKHGSHSV TLSSGLLKSGQ+CN+MN
Sbjct: 481 LNILGESTYRIIVSLQMQETKLFKAEPKVTPGYGSKHGSHSVSTLSSGLLKSGQICNYMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RF ITWK++DENEDNSI +AASCNT+CK+C LGCDMELEVRHMTESDWLYLSK SECTV+
Sbjct: 541 RFFITWKVKDENEDNSISDAASCNTICKSCALGCDMELEVRHMTESDWLYLSKFSECTVE 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           +F DQRDTS+S + QTME+ + CL YA+SSAR+ALVLLKLIPVAAR+ LPVLVN +GLLL
Sbjct: 601 NFHDQRDTSASSKEQTMENASRCLKYASSSARKALVLLKLIPVAARKGLPVLVNQKGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSI+FSHCPWLMASAEFKPRVPFGGG SSFM
Sbjct: 661 SIPSIKFSHCPWLMASAEFKPRVPFGGGQSSFM 693

BLAST of MC06g0683 vs. ExPASy TrEMBL
Match: A0A6J1I6Q1 (tRNA(Ile)-2-lysyl-cytidine synthase OS=Cucurbita maxima OX=3661 GN=LOC111471651 PE=3 SV=1)

HSP 1 Score: 1146 bits (2964), Expect = 0.0
Identity = 569/693 (82.11%), Postives = 615/693 (88.74%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLPRLRLLPLSRCFCRHSSVQEPVD 60
           MAQ LIP  Q+ TNHSFFL S+KSKF SPR +FPF LPRLRLLPLSRCFCR+SS+QEP+D
Sbjct: 1   MAQSLIPRAQSGTNHSFFLLSSKSKFSSPRFQFPFMLPRLRLLPLSRCFCRYSSLQEPID 60

Query: 61  LCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDG 120
           +C YREAFSKRMDMAGLKPHHRIALGVSGGPDSMALC L A WK D LN TGK S FI G
Sbjct: 61  MCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKTDSLNGTGKSSTFIGG 120

Query: 121 LLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQM 180
           LLAIVVDHGLRAESRDEA VV NRVT +GIRCEIASCDW DG+PKQGHLQEAARDMRYQM
Sbjct: 121 LLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQM 180

Query: 181 LQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSYSEVS 240
           LQKVCVQ KIGVILVAHHADDQ                         IFS H YSYSEVS
Sbjct: 181 LQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVS 240

Query: 241 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 300
           KN GILLVRPLM+FSKDD+YKICQGANQDWVEDPTNRSSLFARNRIRMSLGD+SSCTLKS
Sbjct: 241 KNYGILLVRPLMEFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKS 300

Query: 301 ELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLV 360
           EL AIISACRKTRLF+D  CLSLAN TLTITELGYAIINLEILNQLKI+DICLSKF+SLV
Sbjct: 301 ELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNQLKIQDICLSKFLSLV 360

Query: 361 LQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPL 420
           LQFISQRHRPVRGNTAKLVLDYI+TVPC+TSLTAAGCYI PAPGSKGTRCI+S SVDCPL
Sbjct: 361 LQFISQRHRPVRGNTAKLVLDYIQTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPL 420

Query: 421 PSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKR 480
           PSA+EVFH PSY+EE+H IS GLEQIINDGKSYLEHFIPKASDVHFLD++PESVLDEAKR
Sbjct: 421 PSAVEVFHVPSYKEERHAISGGLEQIINDGKSYLEHFIPKASDVHFLDTTPESVLDEAKR 480

Query: 481 LNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMN 540
           LNI+ ESTYRII+SLQMQET+LFKA+ K TPGYE+KHGSHSV TLSSGLLK GQ+CN+MN
Sbjct: 481 LNILGESTYRIIVSLQMQETQLFKAEPKVTPGYETKHGSHSVSTLSSGLLKWGQICNYMN 540

Query: 541 RFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSECTVD 600
           RF ITWK++DENEDNSI +AASCNT+CK C LGCDMELEVRHMTES+WLYLSKLSE TV+
Sbjct: 541 RFFITWKVKDENEDNSISDAASCNTICKCCALGCDMELEVRHMTESNWLYLSKLSEGTVE 600

Query: 601 DFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLL 660
           +F DQRDTS+S +  TME+ + CL YA+SSAR+ALVLLKLIPVAAR+SLPVLVN +GLLL
Sbjct: 601 NFHDQRDTSASSKEPTMENASRCLKYASSSARKALVLLKLIPVAARKSLPVLVNQKGLLL 660

Query: 661 SIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           SIPSI+FSHCPWLMASAEFKPRVPFGGGHSSFM
Sbjct: 661 SIPSIKFSHCPWLMASAEFKPRVPFGGGHSSFM 693

BLAST of MC06g0683 vs. ExPASy TrEMBL
Match: A0A1S3CJ33 (tRNA(Ile)-2-lysyl-cytidine synthase OS=Cucumis melo OX=3656 GN=LOC103500981 PE=3 SV=1)

HSP 1 Score: 1110 bits (2870), Expect = 0.0
Identity = 567/699 (81.12%), Postives = 609/699 (87.12%), Query Frame = 0

Query: 1   MAQGLIPWPQTRTNHSFFLFSTKSKFPSPRCKFPFRLP----RLRLLPLSRCFCRHSSVQ 60
           MAQGLI   QT TNHSFF  S+KSKFP   CKFPF LP    RLRLLPL RCFC +SS+Q
Sbjct: 1   MAQGLI---QTGTNHSFFFSSSKSKFPLQICKFPFILPSLRLRLRLLPLPRCFCSYSSLQ 60

Query: 61  EPVDLCNYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSN 120
           + +DL NYREAFSKRMDMAGLKPHH IALGVSGGPDSMALC LTA WKMDGL  + K S 
Sbjct: 61  QSIDLSNYREAFSKRMDMAGLKPHHHIALGVSGGPDSMALCVLTAGWKMDGLGTSCKSST 120

Query: 121 FIDGLLAIVVDHGLRAESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDM 180
           F+DGLLAIVVDHGLRAESR EA+VV+NRVT LGIRCE+ASCDW +GRPKQGHLQEAARDM
Sbjct: 121 FLDGLLAIVVDHGLRAESRVEAQVVYNRVTQLGIRCELASCDWINGRPKQGHLQEAARDM 180

Query: 181 RYQMLQKVCVQQKIGVILVAHHADDQ-------------------------IFSSHTYSY 240
           RYQMLQKVCVQ KIGVILVAHHADDQ                         IFSSHTYSY
Sbjct: 181 RYQMLQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSSHTYSY 240

Query: 241 SEVSKNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSC 300
           SEVSKN G+LLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSS 
Sbjct: 241 SEVSKNYGVLLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSS 300

Query: 301 TLKSELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKF 360
           TL SEL AIISACRKTRLFIDQVCLSLAN TLTITE+GYAIINLEILNQLKI DICL+KF
Sbjct: 301 TLNSELHAIISACRKTRLFIDQVCLSLANWTLTITEVGYAIINLEILNQLKIPDICLTKF 360

Query: 361 VSLVLQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSV 420
           VSLVLQFISQRHRPVRGNTAKLVLDYI+TVPCKTSLTAAGCYI PAPGSKGTRCI+  SV
Sbjct: 361 VSLVLQFISQRHRPVRGNTAKLVLDYIQTVPCKTSLTAAGCYICPAPGSKGTRCIVCCSV 420

Query: 421 DCPLPSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLD 480
           DCPLPS IEVFHAPSY++E++D+SDGLE+IINDGKSYLEH+IP+ASDVHFLD++PE+VLD
Sbjct: 421 DCPLPSKIEVFHAPSYKKERNDVSDGLEKIINDGKSYLEHYIPEASDVHFLDATPETVLD 480

Query: 481 EAKRLNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMC 540
           EAKRLNIISESTYRII+SLQMQETKLFKA+ KFTPG+ESKHGSHSV+T  SGLLK GQ+C
Sbjct: 481 EAKRLNIISESTYRIIVSLQMQETKLFKAEPKFTPGHESKHGSHSVRTSFSGLLKPGQIC 540

Query: 541 NFMNRFSITWKIRDENEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSE 600
           N+MNRF ITWKI+DENE N+ F+AA CN  CK+CVLGC MELEVRHMTESDWLYLSKLSE
Sbjct: 541 NYMNRFFITWKIKDENEVNT-FKAAGCNAFCKSCVLGCHMELEVRHMTESDWLYLSKLSE 600

Query: 601 C--TVDDFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVN 660
           C  TV + Q+ RDT +SLE QTMES N CLN A SSAR ALVLLKLIPVAARRSLPVLVN
Sbjct: 601 CSCTVKNCQNPRDTLASLEGQTMESTNRCLNSARSSAREALVLLKLIPVAARRSLPVLVN 660

Query: 661 HQGLLLSIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 668
           HQGLLLSIP IRFSHCPWL+ASAEFKP VPFGGGHSSF+
Sbjct: 661 HQGLLLSIPCIRFSHCPWLVASAEFKPMVPFGGGHSSFL 695

BLAST of MC06g0683 vs. ExPASy TrEMBL
Match: A0A5A7TYZ5 (tRNA(Ile)-2-lysyl-cytidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00330 PE=3 SV=1)

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 516/624 (82.69%), Postives = 554/624 (88.78%), Query Frame = 0

Query: 72  MDMAGLKPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLR 131
           MDMAGLKPHH IALGVSGGPDSMALC LTA WKMDGL  + K S F+DGLLAIVVDHGLR
Sbjct: 1   MDMAGLKPHHHIALGVSGGPDSMALCVLTAGWKMDGLGTSCKSSTFLDGLLAIVVDHGLR 60

Query: 132 AESRDEAKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIG 191
           AESR EA+VV+NRVT LGIRCE+ASCDW +GRPKQGHLQEAARDMRYQMLQKVCVQ KIG
Sbjct: 61  AESRVEAQVVYNRVTQLGIRCELASCDWINGRPKQGHLQEAARDMRYQMLQKVCVQHKIG 120

Query: 192 VILVAHHADDQ-------------------------IFSSHTYSYSEVSKNCGILLVRPL 251
           VILVAHHADDQ                         IFSSHTYSYSEVSKN G+LLVRPL
Sbjct: 121 VILVAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSSHTYSYSEVSKNYGVLLVRPL 180

Query: 252 MDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKSELRAIISACRK 311
           MDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSS TL SEL AIISACRK
Sbjct: 181 MDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSSTLNSELHAIISACRK 240

Query: 312 TRLFIDQVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPV 371
           TRLFIDQVCLSLAN TLTITE+GYAIINLEILNQLKI DICL+KFVSLVLQFISQRHRPV
Sbjct: 241 TRLFIDQVCLSLANWTLTITEVGYAIINLEILNQLKIPDICLTKFVSLVLQFISQRHRPV 300

Query: 372 RGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPLPSAIEVFHAPS 431
           RGNTAKLVLDYI+TVPCKTSLTAAGCYI PAPGSKGTRCI+  SVDCPLPS IEVFHAPS
Sbjct: 301 RGNTAKLVLDYIQTVPCKTSLTAAGCYICPAPGSKGTRCIVCCSVDCPLPSKIEVFHAPS 360

Query: 432 YREEKHDISDGLEQIINDGKSYLEHFIPKASDVHFLDSSPESVLDEAKRLNIISESTYRI 491
           Y++E++D+SDGLEQIINDGKSYLEH+IP+ASDVHFLD++PE+VLDEAKRLN++SESTYRI
Sbjct: 361 YKKERNDVSDGLEQIINDGKSYLEHYIPEASDVHFLDATPETVLDEAKRLNMLSESTYRI 420

Query: 492 IISLQMQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMNRFSITWKIRDE 551
           I+SLQMQETKLFKA+ KFTPG+ESKHGSHSV+T SSGLLK GQ+CN+MNRF ITWKI+DE
Sbjct: 421 IVSLQMQETKLFKAEPKFTPGHESKHGSHSVRTSSSGLLKPGQICNYMNRFFITWKIKDE 480

Query: 552 NEDNSIFEAASCNTVCKACVLGCDMELEVRHMTESDWLYLSKLSEC--TVDDFQDQRDTS 611
           NE N+ F+AA CN  CK+CVLGC MELEVRHMTESDWLYLSKLSEC  TV + Q+ RDT 
Sbjct: 481 NEVNT-FKAAGCNAFCKSCVLGCHMELEVRHMTESDWLYLSKLSECSCTVKNCQNPRDTL 540

Query: 612 SSLELQTMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLLSIPSIRFSH 668
           +SLE QTMESPN CLN A SSAR ALVLLKLIPVAARRSLPVLVNHQGLLLSIP IRFSH
Sbjct: 541 ASLEGQTMESPNRCLNSARSSAREALVLLKLIPVAARRSLPVLVNHQGLLLSIPCIRFSH 600

BLAST of MC06g0683 vs. TAIR 10
Match: AT3G24560.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 656.4 bits (1692), Expect = 2.5e-188
Identity = 359/678 (52.95%), Postives = 449/678 (66.22%), Query Frame = 0

Query: 20  FSTKSKFPSPRCKFPF--RLPRLRLLPLSRCFCRHSSVQEPVDLCNYREAFSKRMDMAGL 79
           FS K+ F    C +P   R P+       R FC H+ V E VD   Y+E F+KRMDMAGL
Sbjct: 29  FSPKTLF---TCSYPLQSRHPKFS----QRLFCNHACVPETVDETRYKELFNKRMDMAGL 88

Query: 80  KPHHRIALGVSGGPDSMALCALTADWKMDGLNATGKRSNFIDGLLAIVVDHGLRAESRDE 139
           KPH+RIALGVSGGPDSMALC LTA WK +GL+   K   FIDGL+AIVVDHGLR ES+DE
Sbjct: 89  KPHNRIALGVSGGPDSMALCVLTAKWKTEGLSCVNKTDGFIDGLVAIVVDHGLRQESKDE 148

Query: 140 AKVVWNRVTNLGIRCEIASCDWSDGRPKQGHLQEAARDMRYQMLQKVCVQQKIGVILVAH 199
           A++V +RV+ +GIRCEIASCDW DGRPK GHLQEAAR+MRY+M+  VC +Q+I V+L+AH
Sbjct: 149 AELVCSRVSQMGIRCEIASCDWVDGRPKLGHLQEAAREMRYEMISNVCFRQQIEVLLIAH 208

Query: 200 HADDQ-------------------------IFSSHTYSYSEVSKNCGILLVRPLMDFSKD 259
           HADDQ                         IFS +    ++  KN  I LVRPL+D  K+
Sbjct: 209 HADDQAELFILRLSRSSGVLGLAGTAFASEIFSRNLQLDAKHMKNQSIRLVRPLLDLWKE 268

Query: 260 DMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKSELRAIISACRKTRLFID 319
           DMYKICQ   QDWVEDPTNRS LF RNRIR S+G+L S + KSEL+A+IS CR+TR F+D
Sbjct: 269 DMYKICQWGRQDWVEDPTNRSQLFVRNRIRTSIGNLESGSFKSELQAVISECRRTRSFVD 328

Query: 320 QVCLSLANQTLTITELGYAIINLEILNQLKIKDICLSKFVSLVLQFISQRHRPVRGNTAK 379
           +VC  L +QT+T+T+ GYAI++LE LN   +KDICLSK++  VLQFISQR RP+RGNT+K
Sbjct: 329 KVCTDLIHQTVTVTDKGYAILDLERLNPSGVKDICLSKYLFAVLQFISQRQRPIRGNTSK 388

Query: 380 LVLDYIRTVPCKTSLTAAGCYISPAPGSKGTRCIISSSVDCPLPSAIEVFHAPSYREEKH 439
           L+L+YIR +PC+TSLTAAGCY+SPAPGSKGT+ I+S SVDCPLPS  E+ +  S+ E   
Sbjct: 389 LLLNYIRAIPCRTSLTAAGCYLSPAPGSKGTKIIVSCSVDCPLPSKTELLNI-SFNETP- 448

Query: 440 DISDGLEQIINDGKSYLEHFIPKA-SDVHFLDSSPESVLDEAKRLNIISESTYRIIISLQ 499
             SD L QII D KS+ +H  P +  +V FLD + ESVL +A+ LN++SESTY  I  LQ
Sbjct: 449 --SDDLGQIIADAKSFSDHVAPTSLFEVQFLDVASESVLSKARELNLLSESTYTTIGLLQ 508

Query: 500 MQETKLFKADSKFTPGYESKHGSHSVKTLSSGLLKSGQMCNFMNRFSITWKIRDENEDNS 559
             ETK F   ++     E +HG++   +     L  GQ   FMNRF I W + D      
Sbjct: 509 RDETKRFLTKTE-EKSVELEHGTNIAASSDKVHLCLGQNLYFMNRFLIRWNLSDH----- 568

Query: 560 IFEAASCNTV-CKACVLGCDMELEVRHMTESDWLYLSKLSECTVDDFQDQRDTSSSLELQ 619
                 CN   C+ C +     +EVRHM E DWLYL++LS+C+                 
Sbjct: 569 -----QCNEADCRNCPVSTATSMEVRHMVEPDWLYLAELSKCSTS--------------- 628

Query: 620 TMESPNGCLNYATSSARRALVLLKLIPVAARRSLPVLVNHQGLLLSIPSIRFSHCPWLMA 669
                    N++ SS+++AL  LKLIP AAR+SLPVLVNH GLLL IP+I FS+C  L A
Sbjct: 629 ---------NHSISSSQKALRSLKLIPAAARKSLPVLVNHCGLLLCIPAIGFSYCSCLEA 660

BLAST of MC06g0683 vs. TAIR 10
Match: AT3G24560.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 435.6 bits (1119), Expect = 7.0e-122
Identity = 242/469 (51.60%), Postives = 310/469 (66.10%), Query Frame = 0

Query: 202 QIFSSHTYSYSEVSKNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRI 261
           +IFS +    ++  KN  I LVRPL+D  K+DMYKICQ   QDWVEDPTNRS LF RNRI
Sbjct: 33  EIFSRNLQLDAKHMKNQSIRLVRPLLDLWKEDMYKICQWGRQDWVEDPTNRSQLFVRNRI 92

Query: 262 RMSLGDLSSCTLKSELRAIISACRKTRLFIDQVCLSLANQTLTITELGYAIINLEILNQL 321
           R S+G+L S + KSEL+A+IS CR+TR F+D+VC  L +QT+T+T+ GYAI++LE LN  
Sbjct: 93  RTSIGNLESGSFKSELQAVISECRRTRSFVDKVCTDLIHQTVTVTDKGYAILDLERLNPS 152

Query: 322 KIKDICLSKFVSLVLQFISQRHRPVRGNTAKLVLDYIRTVPCKTSLTAAGCYISPAPGSK 381
            +KDICLSK++  VLQFISQR RP+RGNT+KL+L+YIR +PC+TSLTAAGCY+SPAPGSK
Sbjct: 153 GVKDICLSKYLFAVLQFISQRQRPIRGNTSKLLLNYIRAIPCRTSLTAAGCYLSPAPGSK 212

Query: 382 GTRCIISSSVDCPLPSAIEVFHAPSYREEKHDISDGLEQIINDGKSYLEHFIPKA-SDVH 441
           GT+ I+S SVDCPLPS  E+ +  S+ E     SD L QII D KS+ +H  P +  +V 
Sbjct: 213 GTKIIVSCSVDCPLPSKTELLNI-SFNETP---SDDLGQIIADAKSFSDHVAPTSLFEVQ 272

Query: 442 FLDSSPESVLDEAKRLNIISESTYRIIISLQMQETKLFKADSKFTPGYESKHGSHSVKTL 501
           FLD + ESVL +A+ LN++SESTY  I  LQ  ETK F   ++     E +HG++   + 
Sbjct: 273 FLDVASESVLSKARELNLLSESTYTTIGLLQRDETKRFLTKTE-EKSVELEHGTNIAASS 332

Query: 502 SSGLLKSGQMCNFMNRFSITWKIRDENEDNSIFEAASCNTV-CKACVLGCDMELEVRHMT 561
               L  GQ   FMNRF I W + D            CN   C+ C +     +EVRHM 
Sbjct: 333 DKVHLCLGQNLYFMNRFLIRWNLSDH----------QCNEADCRNCPVSTATSMEVRHMV 392

Query: 562 ESDWLYLSKLSECTVDDFQDQRDTSSSLELQTMESPNGCLNYATSSARRALVLLKLIPVA 621
           E DWLYL++LS+C+                          N++ SS+++AL  LKLIP A
Sbjct: 393 EPDWLYLAELSKCSTS------------------------NHSISSSQKALRSLKLIPAA 452

Query: 622 ARRSLPVLVNHQGLLLSIPSIRFSHCPWLMASAEFKPRVPFGGGHSSFM 669
           AR+SLPVLVNH GLLL IP+I FS+C  L ASA F PR+P GGGHSSF+
Sbjct: 453 ARKSLPVLVNHCGLLLCIPAIGFSYCSCLEASAVFLPRIPLGGGHSSFL 462

BLAST of MC06g0683 vs. TAIR 10
Match: AT3G24560.3 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.8e-115
Identity = 242/484 (50.00%), Postives = 304/484 (62.81%), Query Frame = 0

Query: 216 KNCGILLVRPLMDFSKDDMYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDLSSCTLKS 275
           KN  I LVRPL+D  K+DMYKICQ   QDWVEDPTNRS LF RNRIR S+G+L S + KS
Sbjct: 2   KNQSIRLVRPLLDLWKEDMYKICQWGRQDWVEDPTNRSQLFVRNRIRTSIGNLESGSFKS 61

Query: 276 ELRAIISACRKTRLFIDQVCLSLANQTLTIT-ELGYAIINLEILNQLKIKDICLSK---- 335
           EL+A+IS CR+TR F+D+VC  L +QT+T+T + GYAI++LE LN   +KDICLSK    
Sbjct: 62  ELQAVISECRRTRSFVDKVCTDLIHQTVTVTDQKGYAILDLERLNPSGVKDICLSKYLFA 121

Query: 336 --------FVSLV----------------LQFISQRHRPVRGNTAKLVLDYIRTVPCKTS 395
                   F SLV                LQFISQR RP+RGNT+KL+L+YIR +PC+TS
Sbjct: 122 VLQVLHLEFFSLVKLLPFECFLDYSLLSNLQFISQRQRPIRGNTSKLLLNYIRAIPCRTS 181

Query: 396 LTAAGCYISPAPGSKGTRCIISSSVDCPLPSAIEVFHAPSYREEKHDISDGLEQIINDGK 455
           LTAAGCY+SPAPGSKGT+ I+S SVDCPLPS  E+ +  S+ E     SD L QII D K
Sbjct: 182 LTAAGCYLSPAPGSKGTKIIVSCSVDCPLPSKTELLNI-SFNETP---SDDLGQIIADAK 241

Query: 456 SYLEHFIPKA-SDVHFLDSSPESVLDEAKRLNIISESTYRIIISLQMQETKLFKADSKFT 515
           S+ +H  P +  +V FLD + ESVL +A+ LN++SESTY  I  LQ  ETK F   ++  
Sbjct: 242 SFSDHVAPTSLFEVQFLDVASESVLSKARELNLLSESTYTTIGLLQRDETKRFLTKTE-E 301

Query: 516 PGYESKHGSHSVKTLSSGLLKSGQMCNFMNRFSITWKIRDENEDNSIFEAASCNTV-CKA 575
              E +HG++   +     L  GQ   FMNRF I W + D            CN   C+ 
Sbjct: 302 KSVELEHGTNIAASSDKVHLCLGQNLYFMNRFLIRWNLSDH----------QCNEADCRN 361

Query: 576 CVLGCDMELEVRHMTESDWLYLSKLSECTVDDFQDQRDTSSSLELQTMESPNGCLNYATS 635
           C +     +EVRHM E DWLYL++LS+C+                          N++ S
Sbjct: 362 CPVSTATSMEVRHMVEPDWLYLAELSKCSTS------------------------NHSIS 421

Query: 636 SARRALVLLKLIPVAARRSLPVLVNHQGLLLSIPSIRFSHCPWLMASAEFKPRVPFGGGH 669
           S+++AL  LKLIP AAR+SLPVLVNH GLLL IP+I FS+C  L ASA F PR+P GGGH
Sbjct: 422 SSQKALRSLKLIPAAARKSLPVLVNHCGLLLCIPAIGFSYCSCLEASAVFLPRIPLGGGH 446