MC06g0662 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0662
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Heavy metal-associated domain containing protein
Location	MC06: 5475883 .. 5477547 (-)
RNA-Seq Expression	MC06g0662
Synteny	MC06g0662

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTGAAGTGATGGGCTCCAATAATTGAAGCCCCCAGCGGCCATCTCCTCATTTCATGCCACTATAATATATATATATATATATAAGTAATGGACGAAGACAAGGCTGTCAGCCCAAACAGTCCCGTCACCCGCAGGTGGACGGTGAGTGCCCAAAAAGCCTTAGCTTTCGCTTCCTGTTCTGCTAAGTGCTAACTTCAACCGCAGCAATCATTTCGTTTCATGCTCCTCCAATCCAACCCAATTTCAGGAATTTATTTTATTTATTGGCTTATTCATTATTTGGGAAAGGCTGGTTAGCACTTGGCCATTATTGTTCCCCCTTTATTATTTGCTTCATTTGGCCACCATTACCAAATGTTTCTTCTCTTCACTTCTCTTCTCTCCGCCTTTATTTTCGCCATTTTGCTATAAATACATCAATACCCTAAAGTTGCACTTATTTTTCCTACTTGAAATATTATATATTTCATATCACTGTACCTGCTGCCGTGTCGTCTGAGATCATGGGCAGGGTTATTATGATATTCATCACTTGATCTTTGCAGTTGGGGGATTGGTGAGGCAGTTGTGGAAATACATGGAGAGGCCTTAAAAGTTAAAAGGGTGTCTGTGAAAATCACAAATTTGAAGCCTTTTTAGTTCATTACTATGATAATGGGGAAGAAGCTGAAGAAAATTGCAAGTGTTTTGGATTGTTTCAGTAGTGCTTCTTCATGTAGCTCTTGTTTTTGCATAAACTCCATGGAAGATCAGCAAGAAGATGAGTTTGATAAAGAACCGCTCATGCCTTTGCCTACCCACAACCAGAAGGAGCAGTCGCTGAGATTGAAGGACCTTGTCAATGGAAACCAAACCTTAGCCTTCCAACTTAAGCCCAAGGTAATTTTATTAATTAATTAATTTCCCTGCCCGTCCTTTTTCTTTCACCGTTTCATAAAGGAAATGCAAACAAAGTTTTAGATTGATTGGGGAAATATAAAATAAAACCGTGGAGATATTTAAGATATTTGAGACCCTGTTCTGCGGTGGGTTTGATTTTGCAGATGGTGATGTTGAGGGTGTCCATGCACTGTAGAGGCTGTGCAAGAAAAGTGGAGAAGCACATTTCAAAGATGGAAGGTAACACATAGCCATCAAATTAACAACTCCCACAAATTAACAAAAAAAATATAATTATGTTGGGATTTTTTTTTTCTTTGAATGATTTACAGGAGTGAGCTCGTACACAATAGACTTGGAGACGAAAATGGTGATTATTATTGGAGACATTCTGCCTTTTGAAGTGGTGGAGAGCGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCTCTAGCTTGACCATTATTATTATTTGCATATGAGCAAATAATTAATATTATTTATTGTAACATCAATTAGAAATGTGTACATCGTTCTAAAACAGAGGGATTCGAATTGGCAGAGAATATTTAATATTATATTGTGTGTAATTTCGTGTTTTTAATTTCCATATTTGACCACTTGTGGTGTGAATGTGATTACAACCAACGTGACCTAAGAGATTGAACCACTTAATTTAGGTTAATTGTTGACATTATTGTAAATATTTATCATTTTCTACATGTAGCTATGTCTTTTTTTTTTTAGTAAAATAACATATGAGACGAGGATTTGAA

mRNA sequence

ATGAAATTGAAGTGATGGGCTCCAATAATTGAAGCCCCCAGCGGCCATCTCCTCATTTCATGCCACTATAATATATATATATATATATAAGTAATGGACGAAGACAAGGCTGTCAGCCCAAACAGTCCCGTCACCCGCAGGTGGACGGTGAGTGCCCAAAAAGCCTTAGCTTTCGCTTCCTGTTCTGCTAAGTGCTAACTTCAACCGCAGCAATCATTTCGTTTCATGCTCCTCCAATCCAACCCAATTTCAGGAATTTATTTTATTTATTGGCTTATTCATTATTTGGGAAAGGCTGGTTAGCACTTGGCCATTATTGTTCCCCCTTTATTATTTGCTTCATTTGGCCACCATTACCAAATGTTTCTTCTCTTCACTTCTCTTCTCTCCGCCTTTATTTTCGCCATTTTGCTATAAATACATCAATACCCTAAAGTTGCACTTATTTTTCCTACTTGAAATATTATATATTTCATATCACTGTACCTGCTGCCGTGTCGTCTGAGATCATGGGCAGGGTTATTATGATATTCATCACTTGATCTTTGCAGTTGGGGGATTGGTGAGGCAGTTGTGGAAATACATGGAGAGGCCTTAAAAGTTAAAAGGGTGTCTGTGAAAATCACAAATTTGAAGCCTTTTTAGTTCATTACTATGATAATGGGGAAGAAGCTGAAGAAAATTGCAAGTGTTTTGGATTGTTTCAGTAGTGCTTCTTCATGTAGCTCTTGTTTTTGCATAAACTCCATGGAAGATCAGCAAGAAGATGAGTTTGATAAAGAACCGCTCATGCCTTTGCCTACCCACAACCAGAAGGAGCAGTCGCTGAGATTGAAGGACCTTGTCAATGGAAACCAAACCTTAGCCTTCCAACTTAAGCCCAAGATGGTGATGTTGAGGGTGTCCATGCACTGTAGAGGCTGTGCAAGAAAAGTGGAGAAGCACATTTCAAAGATGGAAGGAGTGAGCTCGTACACAATAGACTTGGAGACGAAAATGGTGATTATTATTGGAGACATTCTGCCTTTTGAAGTGGTGGAGAGCGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCTCTAGCTTGACCATTATTATTATTTGCATATGAGCAAATAATTAATATTATTTATTGTAACATCAATTAGAAATGTGTACATCGTTCTAAAACAGAGGGATTCGAATTGGCAGAGAATATTTAATATTATATTGTGTGTAATTTCGTGTTTTTAATTTCCATATTTGACCACTTGTGGTGTGAATGTGATTACAACCAACGTGACCTAAGAGATTGAACCACTTAATTTAGGTTAATTGTTGACATTATTGTAAATATTTATCATTTTCTACATGTAGCTATGTCTTTTTTTTTTTAGTAAAATAACATATGAGACGAGGATTTGAA

Coding sequence (CDS)

ATGATAATGGGGAAGAAGCTGAAGAAAATTGCAAGTGTTTTGGATTGTTTCAGTAGTGCTTCTTCATGTAGCTCTTGTTTTTGCATAAACTCCATGGAAGATCAGCAAGAAGATGAGTTTGATAAAGAACCGCTCATGCCTTTGCCTACCCACAACCAGAAGGAGCAGTCGCTGAGATTGAAGGACCTTGTCAATGGAAACCAAACCTTAGCCTTCCAACTTAAGCCCAAGATGGTGATGTTGAGGGTGTCCATGCACTGTAGAGGCTGTGCAAGAAAAGTGGAGAAGCACATTTCAAAGATGGAAGGAGTGAGCTCGTACACAATAGACTTGGAGACGAAAATGGTGATTATTATTGGAGACATTCTGCCTTTTGAAGTGGTGGAGAGCGTCTCCAAAGTCAAAAACGCACAGCTCTGGCAATCTTCTCTAGCTTGA

Protein sequence

MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKNAQLWQSSLA

Homology

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BLAST of MC06g0662 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 48/124 (38.71%), Postives = 70/124 (56.45%), Query Frame = 0


Query: 17  FSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLVNGNQTLAFQLKP 76
           FS  +  S    ++S+ED+     D +   P P      QS         + +       
Sbjct: 196 FSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPPEKNSSS------D 255

Query: 77  KMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKN 136
           ++V+LRVS+HC+GCA KV+KH+SK++GV+SY ID   K V + GD+ P  V+ S+SKVKN
Sbjct: 256 QVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKN 313

Query: 137 AQLW 141
           AQ W
Sbjct: 316 AQFW 313

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BLAST of MC06g0662 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-14
Identity = 42/117 (35.90%), Postives = 67/117 (57.26%), Query Frame = 0


Query: 24  SSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLVNGNQTLAFQLKPKMVMLRV 83
           S+ + + S  D      D++P   +       +   L +     +T       ++V+L+V
Sbjct: 132 STRYLLGSDPDSITGSVDQDPAKTVEAEAPAGEDKTLTE----KKTTCGDTDQQVVVLKV 191

Query: 84  SMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKNAQLW 141
           S+HCRGC  KV KH+++M+GV+S+ ID   K V + GDI P E+++S+SKVKNAQ W
Sbjct: 192 SLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNAQFW 244

[Close]

BLAST of MC06g0662 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.0e-14
Identity = 38/66 (57.58%), Postives = 51/66 (77.27%), Query Frame = 0


Query: 77  KMVMLRVSM--HCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKV 136
           ++V+LRVS+  HCRGC  KV+KH+SKM+GV+S+ ID  +K V + GDI P EV+  +SKV
Sbjct: 168 QVVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKVTVTGDITPLEVLGCLSKV 227

Query: 137 KNAQLW 141
           KNAQ W
Sbjct: 228 KNAQFW 233

[Close]

BLAST of MC06g0662 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.2e-08
Identity = 26/66 (39.39%), Postives = 49/66 (74.24%), Query Frame = 0


Query: 80  MLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKV-KNAQ 139
           +LRVS+HC GC RK++K +SK++GV +  ID++ + V +IG++ P  +++ + K  ++A+
Sbjct: 33  VLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAE 92

Query: 140 LWQSSL 145
           LW +S+
Sbjct: 93  LWPTSM 98

[Close]

BLAST of MC06g0662 vs. ExPASy Swiss-Prot
Match: Q94BT9 (Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.0e-06
Identity = 24/65 (36.92%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 79  VMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKV-KNA 138
           V+LRV+M C GC   V++ + KMEGV S+ +D++ + V + G++ P  V+++V+K  K  
Sbjct: 5   VVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGKKT 64

Query: 139 QLWQS 143
             W++
Sbjct: 65  AFWEA 69

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BLAST of MC06g0662 vs. NCBI nr
Match: XP_022144911.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 281 bits (719), Expect = 2.64e-95
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL 60
           MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL
Sbjct: 1   MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL 60

Query: 61  KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG 120
           KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG
Sbjct: 61  KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG 120

Query: 121 DILPFEVVESVSKVKNAQLWQSSLA 145
           DILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 DILPFEVVESVSKVKNAQLWQSSLA 145

[Close]

BLAST of MC06g0662 vs. NCBI nr
Match: XP_038880121.1 (heavy metal-associated isoprenylated plant protein 45 [Benincasa hispida])

HSP 1 Score: 213 bits (542), Expect = 2.61e-68
Identity = 117/147 (79.59%), Postives = 128/147 (87.07%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFS--SASSCSSCFCINSMEDQQEDE--FDKEPLMPLPTHNQKEQSL 62
           MGKKLKK+A V DCFS  S +S +SCFCINSME Q E++  FDK+PL  + T+ +  Q L
Sbjct: 1   MGKKLKKMAKVFDCFSPSSCTSSTSCFCINSMEVQDEEDEFFDKQPL--IATNTKDNQLL 60

Query: 63  RLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVII 122
            LKD+VNGNQTLAFQLKPKMV LRVSMHC+GCARKVEKHISKMEGVSSYTIDLETKMVII
Sbjct: 61  TLKDVVNGNQTLAFQLKPKMVTLRVSMHCKGCARKVEKHISKMEGVSSYTIDLETKMVII 120

Query: 123 IGDILPFEVVESVSKVKNAQLWQSSLA 145
           IGDILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IGDILPFEVVESVSKVKNAQLWQSSLA 145

[Close]

BLAST of MC06g0662 vs. NCBI nr
Match: KAG6587944.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 212 bits (540), Expect = 4.77e-68
Identity = 122/148 (82.43%), Postives = 128/148 (86.49%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFSSA---SSCSS--CFCINSMEDQQEDEFDKEPLMPLPTHNQKEQS 62
           MGK  KKI+  LDCFS +   SSCSS  CFCINSME +QEDEFDK PL  +PTH  K+Q 
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSME-EQEDEFDKLPL--IPTH--KDQL 60

Query: 63  LRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 122
             LKD+VNGNQTLAFQLKPKMV LRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI
Sbjct: 61  PTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 120

Query: 123 IIGDILPFEVVESVSKVKNAQLWQSSLA 145
           IIGDILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IIGDILPFEVVESVSKVKNAQLWQSSLA 143

[Close]

BLAST of MC06g0662 vs. NCBI nr
Match: XP_023531514.1 (heavy metal-associated isoprenylated plant protein 45-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 211 bits (536), Expect = 1.94e-67
Identity = 119/148 (80.41%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFSSA---SSCSS--CFCINSMEDQQEDEFDKEPLMPLPTHNQKEQS 62
           MGK  KKI+  LDCFS +   SSCSS  CFCINSME +QEDEFDK PL+P    + K+Q 
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSME-EQEDEFDKLPLIP----SHKDQL 60

Query: 63  LRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 122
             LKD+VNGNQTLAFQLKPKMV LRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI
Sbjct: 61  PTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 120

Query: 123 IIGDILPFEVVESVSKVKNAQLWQSSLA 145
           IIGD+LPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IIGDVLPFEVVESVSKVKNAQLWQSSLA 143

[Close]

BLAST of MC06g0662 vs. NCBI nr
Match: XP_022973748.1 (heavy metal-associated isoprenylated plant protein 45-like [Cucurbita maxima])

HSP 1 Score: 209 bits (532), Expect = 7.89e-67
Identity = 121/148 (81.76%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFSSA---SSCSS--CFCINSMEDQQEDEFDKEPLMPLPTHNQKEQS 62
           MGK  KKI+  LDCFS +   SSCSS  CFCINSME +QEDEFDK PL  + TH  K+Q 
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSME-EQEDEFDKLPL--IATH--KDQL 60

Query: 63  LRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 122
             LKD+VNGNQTLAFQLKPKMV LRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI
Sbjct: 61  PTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 120

Query: 123 IIGDILPFEVVESVSKVKNAQLWQSSLA 145
           IIGDILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IIGDILPFEVVESVSKVKNAQLWQSSLA 143

[Close]

BLAST of MC06g0662 vs. ExPASy TrEMBL
Match: A0A6J1CUT7 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111014475 PE=4 SV=1)

HSP 1 Score: 281 bits (719), Expect = 1.28e-95
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL 60
           MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL
Sbjct: 1   MIMGKKLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRL 60

Query: 61  KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG 120
           KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG
Sbjct: 61  KDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIG 120

Query: 121 DILPFEVVESVSKVKNAQLWQSSLA 145
           DILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 DILPFEVVESVSKVKNAQLWQSSLA 145

[Close]

BLAST of MC06g0662 vs. ExPASy TrEMBL
Match: A0A6J1IE28 (heavy metal-associated isoprenylated plant protein 45-like OS=Cucurbita maxima OX=3661 GN=LOC111472318 PE=4 SV=1)

HSP 1 Score: 209 bits (532), Expect = 3.82e-67
Identity = 121/148 (81.76%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFSSA---SSCSS--CFCINSMEDQQEDEFDKEPLMPLPTHNQKEQS 62
           MGK  KKI+  LDCFS +   SSCSS  CFCINSME +QEDEFDK PL  + TH  K+Q 
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSME-EQEDEFDKLPL--IATH--KDQL 60

Query: 63  LRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 122
             LKD+VNGNQTLAFQLKPKMV LRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI
Sbjct: 61  PTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 120

Query: 123 IIGDILPFEVVESVSKVKNAQLWQSSLA 145
           IIGDILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IIGDILPFEVVESVSKVKNAQLWQSSLA 143

[Close]

BLAST of MC06g0662 vs. ExPASy TrEMBL
Match: A0A6J1EZE4 (heavy metal-associated isoprenylated plant protein 45-like OS=Cucurbita moschata OX=3662 GN=LOC111440501 PE=4 SV=1)

HSP 1 Score: 209 bits (532), Expect = 3.82e-67
Identity = 121/148 (81.76%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 3   MGKKLKKIASVLDCFSSA---SSCSS--CFCINSMEDQQEDEFDKEPLMPLPTHNQKEQS 62
           MGK  KKI+  LDCFS +   SSCSS  CFCINSME +QEDEFDK PL  + TH  K+Q 
Sbjct: 1   MGKLHKKISKALDCFSLSLGPSSCSSGSCFCINSME-EQEDEFDKLPL--ISTH--KDQL 60

Query: 63  LRLKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 122
             LKD+VNGNQTLAFQLKPKMV LRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI
Sbjct: 61  PTLKDVVNGNQTLAFQLKPKMVTLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVI 120

Query: 123 IIGDILPFEVVESVSKVKNAQLWQSSLA 145
           IIGDILPFEVVESVSKVKNAQLWQSSLA
Sbjct: 121 IIGDILPFEVVESVSKVKNAQLWQSSLA 143

[Close]

BLAST of MC06g0662 vs. ExPASy TrEMBL
Match: A0A0A0LZH7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G384030 PE=4 SV=1)

HSP 1 Score: 203 bits (516), Expect = 1.19e-64
Identity = 113/146 (77.40%), Postives = 128/146 (87.67%), Query Frame = 0


Query: 6   KLKKIASVLDCFS-SASSCSS--CFCINSME--DQQEDEFDKEPLMPLPTHNQKEQSL-R 65
           KLKK+  V DCFS S++SCSS  CFCINSME  D++++ FDK+PL  +  +N+K+  L  
Sbjct: 3   KLKKMGKVFDCFSYSSNSCSSNSCFCINSMEIEDEEDEFFDKQPL--IANNNRKDNQLPT 62

Query: 66  LKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIII 125
           LKD+VNGNQTLAFQLKPKMV LRVSMHC+GCARKVEKHISKMEGVSSYTIDLETKMVIII
Sbjct: 63  LKDVVNGNQTLAFQLKPKMVTLRVSMHCKGCARKVEKHISKMEGVSSYTIDLETKMVIII 122

Query: 126 GDILPFEVVESVSKVKNAQLWQSSLA 145
           GDILPFEVVESVSKVKNAQLWQSS+A
Sbjct: 123 GDILPFEVVESVSKVKNAQLWQSSIA 146

[Close]

BLAST of MC06g0662 vs. ExPASy TrEMBL
Match: A0A1S3CJJ6 (uncharacterized protein LOC103501543 OS=Cucumis melo OX=3656 GN=LOC103501543 PE=4 SV=1)

HSP 1 Score: 202 bits (513), Expect = 3.41e-64
Identity = 113/146 (77.40%), Postives = 128/146 (87.67%), Query Frame = 0


Query: 6   KLKKIASVLDCFS-SASSCSS--CFCINSM--EDQQEDEFDKEPLMPLPTHNQKE-QSLR 65
           KLKK+  V DCFS S++SCSS  CFCINSM  ED++++ FDK+PL  +  +N+K+ Q L 
Sbjct: 3   KLKKMGKVFDCFSYSSNSCSSNSCFCINSMKIEDEEDEFFDKQPL--IADNNKKDNQLLT 62

Query: 66  LKDLVNGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIII 125
           LKD+VNGNQTLAFQLKPKMV LRVSMHC+GCARKVEKHISKMEGVSSYTIDLE KMVIII
Sbjct: 63  LKDVVNGNQTLAFQLKPKMVTLRVSMHCKGCARKVEKHISKMEGVSSYTIDLEAKMVIII 122

Query: 126 GDILPFEVVESVSKVKNAQLWQSSLA 145
           GDILPFEVVESVSKVKNAQLWQSS+A
Sbjct: 123 GDILPFEVVESVSKVKNAQLWQSSIA 146

[Close]

BLAST of MC06g0662 vs. TAIR 10
Match: AT3G24450.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-34
Identity = 76/139 (54.68%), Postives = 106/139 (76.26%), Query Frame = 0


Query: 6   KLKKIASVLDCFSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLV- 65
           KL+KI  V DC    ++  SCFC+N++ D +E+EF+KEPL+   T  +  + +RLKD+V 
Sbjct: 3   KLQKIGRVWDCLFLPTNQCSCFCLNTLGD-EEEEFEKEPLIDSST-EKSGKVMRLKDVVA 62

Query: 66  -NGNQTLAFQLKPKMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDIL 125
            +  QTLAF LKPK+V L+VSMHC GCA+KVEKHISK++GV+ Y ++LE+K V++ G+IL
Sbjct: 63  ADHRQTLAFHLKPKIVELKVSMHCYGCAKKVEKHISKLDGVTWYKVELESKKVVVKGNIL 122

Query: 126 PFEVVESVSKVKNAQLWQS 143
           P +V+ES+ KVKNAQLW S
Sbjct: 123 PVDVLESICKVKNAQLWSS 139

[Close]

BLAST of MC06g0662 vs. TAIR 10
Match: AT2G28660.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-19
Identity = 45/67 (67.16%), Postives = 55/67 (82.09%), Query Frame = 0


Query: 77  KMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKN 136
           ++V+LRVS+HC+GC  KV KHISKMEGV+SYTIDL TK V ++G I P  +VES+SKVK 
Sbjct: 183 QVVVLRVSIHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVGKITPVGLVESISKVKF 242

Query: 137 AQLWQSS 144
           AQLW SS
Sbjct: 243 AQLWPSS 249

[Close]

BLAST of MC06g0662 vs. TAIR 10
Match: AT2G37390.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 84.3 bits (207), Expect = 8.6e-17
Identity = 35/64 (54.69%), Postives = 51/64 (79.69%), Query Frame = 0


Query: 77  KMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKN 136
           ++V+L+VS+HCRGC  KV KH+++M+GV+S+ ID   K V + GDI P E+++S+SKVKN
Sbjct: 180 QVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKN 239

Query: 137 AQLW 141
           AQ W
Sbjct: 240 AQFW 243

[Close]

BLAST of MC06g0662 vs. TAIR 10
Match: AT5G02600.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.9e-16
Identity = 48/124 (38.71%), Postives = 70/124 (56.45%), Query Frame = 0


Query: 17  FSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLVNGNQTLAFQLKP 76
           FS  +  S    ++S+ED+     D +   P P      QS         + +       
Sbjct: 196 FSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPPEKNSSS------D 255

Query: 77  KMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKN 136
           ++V+LRVS+HC+GCA KV+KH+SK++GV+SY ID   K V + GD+ P  V+ S+SKVKN
Sbjct: 256 QVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKN 313

Query: 137 AQLW 141
           AQ W
Sbjct: 316 AQFW 313

[Close]

BLAST of MC06g0662 vs. TAIR 10
Match: AT5G02600.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.9e-16
Identity = 48/124 (38.71%), Postives = 70/124 (56.45%), Query Frame = 0


Query: 17  FSSASSCSSCFCINSMEDQQEDEFDKEPLMPLPTHNQKEQSLRLKDLVNGNQTLAFQLKP 76
           FS  +  S    ++S+ED+     D +   P P      QS         + +       
Sbjct: 196 FSEPTKASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQSSPPSPPEKNSSS------D 255

Query: 77  KMVMLRVSMHCRGCARKVEKHISKMEGVSSYTIDLETKMVIIIGDILPFEVVESVSKVKN 136
           ++V+LRVS+HC+GCA KV+KH+SK++GV+SY ID   K V + GD+ P  V+ S+SKVKN
Sbjct: 256 QVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKN 313

Query: 137 AQLW 141
           AQ W
Sbjct: 316 AQFW 313

The following BLAST results are available for this feature:

BLAST of MC06g0662 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDS4	2.7e-15	38.71	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q58FZ0	1.0e-14	35.90	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q8RXH8	3.0e-14	57.58	Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q84J88	3.2e-08	39.39	Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX...	[more]
Q94BT9	3.0e-06	36.92	Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2	[more]

BLAST of MC06g0662 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144911.1	2.64e-95	100.00	protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Momordica charantia]	[more]
XP_038880121.1	2.61e-68	79.59	heavy metal-associated isoprenylated plant protein 45 [Benincasa hispida]	[more]
KAG6587944.1	4.77e-68	82.43	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_023531514.1	1.94e-67	80.41	heavy metal-associated isoprenylated plant protein 45-like [Cucurbita pepo subsp...	[more]
XP_022973748.1	7.89e-67	81.76	heavy metal-associated isoprenylated plant protein 45-like [Cucurbita maxima]	[more]

BLAST of MC06g0662 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CUT7	1.28e-95	100.00	protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1IE28	3.82e-67	81.76	heavy metal-associated isoprenylated plant protein 45-like OS=Cucurbita maxima O...	[more]
A0A6J1EZE4	3.82e-67	81.76	heavy metal-associated isoprenylated plant protein 45-like OS=Cucurbita moschata...	[more]
A0A0A0LZH7	1.19e-64	77.40	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G384030 PE=4 SV...	[more]
A0A1S3CJJ6	3.41e-64	77.40	uncharacterized protein LOC103501543 OS=Cucumis melo OX=3656 GN=LOC103501543 PE=...	[more]

BLAST of MC06g0662 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24450.1	1.2e-34	54.68	Heavy metal transport/detoxification superfamily protein	[more]
AT2G28660.1	1.8e-19	67.16	Chloroplast-targeted copper chaperone protein	[more]
AT2G37390.2	8.6e-17	54.69	Chloroplast-targeted copper chaperone protein	[more]
AT5G02600.2	1.9e-16	38.71	Heavy metal transport/detoxification superfamily protein	[more]
AT5G02600.1	1.9e-16	38.71	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 82..133 e-value: 2.5E-14 score: 53.4
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 77..134 score: 11.576355
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 80..121 e-value: 1.094E-12 score: 57.2305
None	No IPR available	GENE3D	3.30.70.100		coord: 75..138 e-value: 7.2E-17 score: 63.4
None	No IPR available	PANTHER	PTHR46119:SF11	HEAVY METAL TRANSPORT/DETOXIFICATION SUPERFAMILY PROTEIN	coord: 9..142
IPR044526	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3	PANTHER	PTHR46119	OS08G0405700 PROTEIN	coord: 9..142
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 76..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0662.1	MC06g0662.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding