MC06g0656 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0656
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	nudix hydrolase 20, chloroplastic-like
Location	MC06: 5433519 .. 5437397 (+)
RNA-Seq Expression	MC06g0656
Synteny	MC06g0656

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGGCCTCTCCTTCAATTCAACCCATGGCCTGCAAATCTCTGGCTCCACACAGATTTTGTAACTTCCCTGCTATCTCTTTCATCTGCGATGCTTCCATGTTTCTCCGGACGAGGTTTCCAACAACATCTACATTTCGGCCAACAGTTCACCGCTCAATAACTTTCGCCTCGGGTTCTGTATCGGTCGCCAGCAGCAGCTTCACTTGGGAAGACGCTCTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTGACGGGTTACTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGTAGGTGTCTTTCTCCTCTTTTATTCTTGTTTGGCTGTATTAACAAAAGTATCTACGTTCCGGTAGCAATTGTACGCTTTAATTCGTTTTACACTTCGAATTCAGCGCAGGAAAAGCTATAGACTGATGAAATGTGGTTCCATGTCTGTTTGGAATTGACTAAATCTTCTAACAGGATATGCAATCCGAGTTTCTTCCGTTTGTCATCGATAACCAAATTATTGGTTACGTGCACCACGGGTGAGGTTCTTACTGCTATATGTCTCGTAATACACAGCATGCATTGAAGATATTGTCTGTCCATGTAGTCTACTTCGTTTATGTGCACTAATTTCATCAAACTTCGCTTGTGTACTATTGCTATATACAACGGATAGGTTCTATCAGTTTGTTCCAATGTAATTTATGCAGTTTCGCCGAACACTTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCAAGATGATTCTGTTCAATGTGGCGCTTACTTAACCTTGAATGAATCTTTGACGACACCCGAAGATAGGACGCGTGGGGTTGGAGATGTAGTCAAATGCTTGGGCGAAGAAGTTATTCCAGGTACACGGAATGAGGTACTGCTTATGATTTAAATAAATGGTTCAATACTTTTCACTTCCTGTCTGTTTGCAACTGTGAGATTGTATATAAAATACAATTGCCTTTCCTCAGTTTAATGCTCAAAATAAGCTGGTCCTTTTTAGAACAAGATAATGTGCCTTGTGGCTAACTGTGTTCAAGTGAACTCTGTCAGCTATACCCTGTAACTTCGTCCTTTGGTGCACCGGTTTTTTTCTCGCTTGAACGTGCTGCAGCTCCTTATTTTGGAATAAAGGTGCAAATGTTCCCCTTCTATAGCATTGTGGAAACTTATTCTTTCTTGTTTTAATTTACCGTAACAAAATCTGAAGCCCTTATTTTGATCCAGCTTTCTATTTAGGTCCTTCAAAATTGCAGGCTATATGTTGTATGGACTCTGTTTATATAAGCTTGGTTGTTAGGTCTCGATAAATTTGAAACCAGTTAATATTTGATCGTCATTCTGAATATAAATACAAAATCTGAAATAATATTTACAATACTATTCAATACCCACCTACATTGCTGGAAATATTTATTCCACTGCTTAGTTATTCTAACTTCCACCTGCCTTCTCTTGGTGCTTTTCTCATCACAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAGAGGGATGGACAAAAGTTTTTGTGGATAGCAAAACGAAGTCAAATGAAGCAAACTTTTCCTGGAATGTTGGATGAATTAGTAGCTGGAGGGCTGGTTTGTAATATTAGCTAACTATTGTTTAGTTTTCTTGCATGTCATGTCATATATGCGTATGTCCTGTTAAAATGTGAAAAGAAAAAGGTTTTCCCTCCTCTTTTCTTCATGATTTGATGTCATTGATCACATACTCCTTTCATCTTTCAAACATAGCCCTGAAATGTCTTCCGATTGACTGAGGATGAGACTTTTGTTTGCTTTCTGGATGATTGGTTCTCTTATTCCTTTTGTTTCTTCTGCTAGATACGTTTAGTATTTTTCTTTTTAAGTTTTAATAGTACAACAAAATTGTGGTATATTGTTTTGAACCTCAAACTTGAAAAGAAATATGTTTGCCAGAAGCTTGCAGTTATATGAAGATACTTTCTCTTATTACGTTTCTTTATATGCATTTGCCTATTTAGTTTAACTTAGTTAAGGAAGGCAACAGCAATTGATTTTTATTTGTTTGGCTTTCAATTTGGACCATAAGTTGGGATAATTAATAACAAAATTAGATAGGTTCTGATACTAACACATTTATTAACTTTGTAATAAACCCTAACTAAACAACCGGAATTTTTGGTCAAGGCCAAGGCGTGAAATTTGATAATTTCCTAAGAAGAGCAGTTTAGAGTCTGCACAAACATGTTATTTAGAAACGTGCACTTGGATGCCGAACACATCATGCTTATTGTGCCTTTATAGCTAGCCCCTGACATTATGATAGTGACAGCCTCAGGGTGTTCCTTGTGGGGAAAATCTGAGGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCGAATAAGTAAGTTCCTAACATGAACTTAATCATAATTTCATGCCAGTATAACCTTTTATTACATGGCTGTGGTAGACCTAGTGCCTTTACAATGCTTTTGTTTTAGGGCCATATCAGTTGGTGCCGTTTCCTATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGAAGAATCAAGGTTAGCTAACCTGATAATTTTAGTCTACATTATCCTAATAACTCTATATTTTTGTCCCATCCGCGGTGTATGAATGCATGTATATATTTTCAATACATATCATTGTATAGTTAGAAGTTTCATGCCGCTAACTTTGCTGAGTAAACTTTTGGCAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGGAAAACGTTGCAAATGTGATACGGAGGACACAGTTATTCAAACCTAATTGTTCTCTTGTCATCATTGATTTCCTGTTTCGACATGGGTATTATATACTATTTTCTCTATGGCTGTACATTTAACATTTGTTACACGACCATTATTTGCTTAAATTACAACCCCTAGTGTTGGTCTTGACGATAAATATTTGTGGATTTAACCAAATTGTCGGTGCCTCGTGGGTACAAAATTTCTCATCAAGACTGGTGGTACTACGGTATACTATACTAGTTCTTTTACCCTTTCACTAACCTTAAAATTTTGGTAGGAGGTAGAAATGTAGAAAAGGAGATTTTTGTTTTGTTTTTAGATAAATTAAAAGAACTGTCAAATTTTTGTTAATAGCAAGTGTGAAGATAGAACACCCAAGTATTGTTGTTTTATTTTTCTTTCCTCCAGGTCCTGATCATTTTGGTATTAAATCTTTTACCATCTATGTAGTCCATCTCAAGGCATCCATAAAAGTTTATAGTTGTTTGTAGTTAATATATACATTTTTTTCTCTTTTAAAACTTGCAGGTGCATTAAGCCCGAAAGTTTGGGGTACCTGGAGCTACTACAAAGTTTGAAGAGTGGAACTTGCTTCTGAATAAATTCATTCGCCCTACTCCATGAGAGGAATCGGGTCAATCTTATTAAACATCGAACTTGGATGGTTTATACTGTCTGTTGTTTAGCCAGAATTTGCTCGATCAGTAGGTGTATTTGTGAAGACAAACAAACTGGCCTTACATGGCCGTAGAATATAAAATTAGTTTCATCATTTTAGTACCACATTTTATCCGTATTGACCCAATTGAACTTTGAAACAAACACCAAGTGGCTAAGCTAATCTGATCTAGTTTAGTAGATAAAATACTTAATTTCTCTCTCAATCGAAGGTTCAATTTCCATTCTCCCATACTAAAAGTTTTGTAAGGTTGCTGTTTGGCTGACCCTCTTCTCATTTGAGTTAGGTTGGCACATACAGGAGAGTGTTCATACTTGGACAAAGATGACTGACTTTATA

mRNA sequence

GCGGCCTCTCCTTCAATTCAACCCATGGCCTGCAAATCTCTGGCTCCACACAGATTTTGTAACTTCCCTGCTATCTCTTTCATCTGCGATGCTTCCATGTTTCTCCGGACGAGGTTTCCAACAACATCTACATTTCGGCCAACAGTTCACCGCTCAATAACTTTCGCCTCGGGTTCTGTATCGGTCGCCAGCAGCAGCTTCACTTGGGAAGACGCTCTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTGACGGGTTACTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAGTTTCTTCCGTTTGTCATCGATAACCAAATTATTGGTTACGTGCACCACGGTTTCGCCGAACACTTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCAAGATGATTCTGTTCAATGTGGCGCTTACTTAACCTTGAATGAATCTTTGACGACACCCGAAGATAGGACGCGTGGGGTTGGAGATGTAGTCAAATGCTTGGGCGAAGAAGTTATTCCAGGTACACGGAATGAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAGAGGGATGGACAAAAGTTTTTGTGGATAGCAAAACGAAGTCAAATGAAGCAAACTTTTCCTGGAATGTTGGATGAATTAGTAGCTGGAGGGCTGGGTGTTCCTTGTGGGGAAAATCTGAGGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCGAATAAGGCCATATCAGTTGGTGCCGTTTCCTATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGGAAAACGTTGCAAATGTGATACGGAGGACACAGTTATTCAAACCTAATTGTTCTCTTGTCATCATTGATTTCCTGTTTCGACATGGGTGCATTAAGCCCGAAAGTTTGGGGTACCTGGAGCTACTACAAAGTTTGAAGAGTGGAACTTGCTTCTGAATAAATTCATTCGCCCTACTCCATGAGAGGAATCGGGTCAATCTTATTAAACATCGAACTTGGATGGTTTATACTGTCTGTTGTTTAGCCAGAATTTGCTCGATCAGTAGGTGTATTTGTGAAGACAAACAAACTGGCCTTACATGGCCGTAGAATATAAAATTAGTTTCATCATTTTAGTACCACATTTTATCCGTATTGACCCAATTGAACTTTGAAACAAACACCAAGTGGCTAAGCTAATCTGATCTAGTTTAGTAGATAAAATACTTAATTTCTCTCTCAATCGAAGGTTCAATTTCCATTCTCCCATACTAAAAGTTTTGTAAGGTTGCTGTTTGGCTGACCCTCTTCTCATTTGAGTTAGGTTGGCACATACAGGAGAGTGTTCATACTTGGACAAAGATGACTGACTTTATA

Coding sequence (CDS)

GCGGCCTCTCCTTCAATTCAACCCATGGCCTGCAAATCTCTGGCTCCACACAGATTTTGTAACTTCCCTGCTATCTCTTTCATCTGCGATGCTTCCATGTTTCTCCGGACGAGGTTTCCAACAACATCTACATTTCGGCCAACAGTTCACCGCTCAATAACTTTCGCCTCGGGTTCTGTATCGGTCGCCAGCAGCAGCTTCACTTGGGAAGACGCTCTCCACATTTCTTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTGACGGGTTACTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAGTTTCTTCCGTTTGTCATCGATAACCAAATTATTGGTTACGTGCACCACGGTTTCGCCGAACACTTGAAGAAGTATGCGAATGTGTTTACCTTCCCCCAAGATGATTCTGTTCAATGTGGCGCTTACTTAACCTTGAATGAATCTTTGACGACACCCGAAGATAGGACGCGTGGGGTTGGAGATGTAGTCAAATGCTTGGGCGAAGAAGTTATTCCAGGTACACGGAATGAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAGAGGGATGGACAAAAGTTTTTGTGGATAGCAAAACGAAGTCAAATGAAGCAAACTTTTCCTGGAATGTTGGATGAATTAGTAGCTGGAGGGCTGGGTGTTCCTTGTGGGGAAAATCTGAGGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCGAATAAGGCCATATCAGTTGGTGCCGTTTCCTATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCCGTGGAAAACGTTGCAAATGTGATACGGAGGACACAGTTATTCAAACCTAATTGTTCTCTTGTCATCATTGATTTCCTGTTTCGACATGGGTGCATTAAGCCCGAAAGTTTGGGGTACCTGGAGCTACTACAAAGTTTGAAGAGTGGAACTTGCTTCTGA

Protein sequence

AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNEVYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGLGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTCF

Homology

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BLAST of MC06g0656 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.6e-99
Identity = 187/343 (54.52%), Postives = 235/343 (68.51%), Query Frame = 0


Query: 41  TTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNR 100
           ++ +  P  H     A+ +V + SSSFTW+D +     E    + SDLTG+FEK+  CNR
Sbjct: 35  SSMSLSPLRHSRALSAATTVPI-SSSFTWDDVIETGRAEY---NSSDLTGFFEKINRCNR 94

Query: 101 GSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSV--QCGAYLTLNESLTT 160
           GS+   EF+PFVI+ QI+GY+H  F E+L+++ ++FTF Q+ S   +   Y+TLN  L  
Sbjct: 95  GSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQNGSCPDRVDGYVTLNLMLQK 154

Query: 161 PEDRTRGVGDVVKCLGEE-VIPGTRNEV---------------------------YGVQM 220
           PEDRTR V DV+K LG++ +IPG RNE+                           YGV M
Sbjct: 155 PEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVFFSLERAAAPYFGIKGYGVHM 214

Query: 221 NGYVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSI 280
           NGYVERDGQK LWI KRS  K T+PGMLD LVAGGL  G+ CG NL KECEEEAGI R+I
Sbjct: 215 NGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGISCGGNLVKECEEEAGISRAI 274

Query: 281 SNKAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVI 340
           +++AI+VGAVSY DI  Y +KRDVLFCYDL+LPE FVPKNQDGEVE F+L+PV  VA+VI
Sbjct: 275 ADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVI 334

Query: 341 RRTQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTC 352
           ++T  FK NCSLVIIDFLFRHG I+PES GYL+L Q L++  C
Sbjct: 335 KKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQRLRNRDC 373

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BLAST of MC06g0656 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-91
Identity = 174/341 (51.03%), Postives = 224/341 (65.69%), Query Frame = 0


Query: 41  TTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNR 100
           ++S+  P +H S   +  +V + S SFTW D    S  E    + SDLTG+ EKV  CNR
Sbjct: 30  SSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNR 89

Query: 101 GSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPE 160
           G +   EF+PFVI+ QI+GY+H GF ++L+ + ++FTF      Q G ++TLN  L  PE
Sbjct: 90  GLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPE 149

Query: 161 DRTRGVGDVVKCLGEE-VIPGTRNEV---------------------------YGVQMNG 220
           +RTR V  V+K LG + +IPG RNE+                           Y + +NG
Sbjct: 150 ERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNG 209

Query: 221 YVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISN 280
           YVERDGQKFLWI KRS  K T+PG LD LVAGGL  G+   ENL KECEEEAGI + +++
Sbjct: 210 YVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLAD 269

Query: 281 KAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRR 340
           +AI+VG VSY DI  Y + RDVLFCYDL+LP+ FVP NQDGEV+ FRL+PV  VANV+R+
Sbjct: 270 RAIAVGVVSYMDIDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRK 329

Query: 341 TQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTC 352
           T  FK +CSLVIIDFLFRHG I+PES GYL+L + L++G C
Sbjct: 330 TSFFKDSCSLVIIDFLFRHGLIRPESPGYLDLYRRLRNGDC 364

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BLAST of MC06g0656 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-29
Identity = 75/175 (42.86%), Postives = 106/175 (60.57%), Query Frame = 0


Query: 186 YGVQMNGYV--ERDGQKFLWIAKRSQMKQTFPGMLDELVAGGLGVPCG--ENLRKECEEE 245
           YG+ +NGYV   +  +   W+ +RS+ KQT+P MLD ++AGGLG P G  E + KE  EE
Sbjct: 156 YGIHINGYVLDPKSKKVQFWVPRRSKTKQTWPLMLDNIIAGGLGYPYGIYETVLKESMEE 215

Query: 246 AGIPRSISNKAI-SVGAVSY----SDIKDYSYKR-------DVLFCYDLKLPESFVPKNQ 305
           A + +S+    I + G+VSY     DI    + +       +V + YDLKL E  +PK  
Sbjct: 216 ANLEKSVIEDNIKATGSVSYLYFTGDISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPN 275

Query: 306 DGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPES-LGYLELL 344
           DGEVE F L  ++   N +R+ + FKPNC+LV++DFL RHG I PE+   YLEL+
Sbjct: 276 DGEVESFNLFSLQETINALRKKE-FKPNCALVMVDFLIRHGYITPENEPNYLELV 329

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BLAST of MC06g0656 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 7.9e-21
Identity = 58/148 (39.19%), Postives = 87/148 (58.78%), Query Frame = 0


Query: 202 LWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVS 261
           +W+ +RS  KQT+P  LD  VAGG+  G      + KE  EEA +  S  N  I  G VS
Sbjct: 153 IWVPRRSPTKQTWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANLDVSSMN-LIPCGTVS 212

Query: 262 YSDI-KDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNC 321
           Y  + K +  + ++ + +DL + +  +P+  DGEV GF LLP+  V + +   + FKPNC
Sbjct: 213 YIKMEKRHWIQPELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKSFKPNC 272

Query: 322 SLVIIDFLFRHGCIKPESLGYLELLQSL 347
           +LV++DFL RHG I P+   YL+ L+ +
Sbjct: 273 ALVLLDFLIRHGIITPQHPQYLQTLERI 298

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BLAST of MC06g0656 vs. NCBI nr
Match: XP_022144971.1 (nudix hydrolase 20, chloroplastic-like [Momordica charantia])

HSP 1 Score: 686 bits (1769), Expect = 3.92e-248
Identity = 344/373 (92.23%), Postives = 344/373 (92.23%), Query Frame = 0


Query: 9   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 68
           MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT
Sbjct: 1   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 60

Query: 69  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 128
           WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH
Sbjct: 61  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 120

Query: 129 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNE---- 188
           LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNE    
Sbjct: 121 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNELYPV 180

Query: 189 -----------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 248
                                  VYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL
Sbjct: 181 TSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 240

Query: 249 VAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 308
           VAGGL  GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK
Sbjct: 241 VAGGLPQGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 300

Query: 309 LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY 352
           LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY
Sbjct: 301 LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY 360

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BLAST of MC06g0656 vs. NCBI nr
Match: XP_022933802.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 582 bits (1500), Expect = 4.50e-207
Identity = 300/381 (78.74%), Postives = 318/381 (83.46%), Query Frame = 0


Query: 1   AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSV 60
           A  P IQPMACKSLA HRF N   IS I +ASMF R R+PT ST    +HRS TFA  SV
Sbjct: 2   ATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTAST----LHRSKTFACRSV 61

Query: 61  SVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGY 120
           SVASS+FTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+
Sbjct: 62  SVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGH 121

Query: 121 VHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPG 180
           VHHGFA HLKKYANVFTFP+DDSV+ GA L+L+ESL TPEDRT  VG+VVKCLGEE+IPG
Sbjct: 122 VHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPG 181

Query: 181 TRNE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQT 240
            RNE                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQT
Sbjct: 182 IRNEMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241

Query: 241 FPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRD 300
           FPGMLDELVAGGL  GV CGENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRD
Sbjct: 242 FPGMLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRD 301

Query: 301 VLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGC 352
           VLFCYDLKLPESF+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG 
Sbjct: 302 VLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 361

[Close]

BLAST of MC06g0656 vs. NCBI nr
Match: XP_023530783.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023530784.1 nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581 bits (1498), Expect = 9.08e-207
Identity = 300/378 (79.37%), Postives = 317/378 (83.86%), Query Frame = 0


Query: 4   PSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVA 63
           P IQPMACKSLA HRF N   IS I +ASMF R  +PT ST    VHRS TFA  SVSVA
Sbjct: 5   PRIQPMACKSLASHRFRNSLPISSISNASMFSRATYPTAST----VHRSKTFACRSVSVA 64

Query: 64  SSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHH 123
           SS+FTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+VHH
Sbjct: 65  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 124

Query: 124 GFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRN 183
           GFA HLKKYANVFTFP+DDSV+ GA L+L+ESL TPEDRT  VG+VVKCLGEE+IPG RN
Sbjct: 125 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 184

Query: 184 E---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQTFPG 243
           E                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQTFPG
Sbjct: 185 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 244

Query: 244 MLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLF 303
           MLDELVAGGL  GV CGENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRDVLF
Sbjct: 245 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 304

Query: 304 CYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKP 352
           CYDLKLPESF+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG IKP
Sbjct: 305 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 364

[Close]

BLAST of MC06g0656 vs. NCBI nr
Match: XP_038878620.1 (nudix hydrolase 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 578 bits (1490), Expect = 1.56e-205
Identity = 299/381 (78.48%), Postives = 314/381 (82.41%), Query Frame = 0


Query: 1   AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSV 60
           A SP I  MACKSLA H FC   A S IC+ASMF  TRF T  TFR     S+TFA  SV
Sbjct: 4   ATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFR-----SMTFAFRSV 63

Query: 61  SVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGY 120
           SV SSSFTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+ I+GY
Sbjct: 64  SVTSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGY 123

Query: 121 VHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPG 180
           VHHGF  HLKKYANVFTFP+DDSV+ GA LTL+ESL TPEDRT  VGDVVKCLGEEVIPG
Sbjct: 124 VHHGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPG 183

Query: 181 TRNE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQT 240
           TRNE                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQT
Sbjct: 184 TRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 243

Query: 241 FPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRD 300
           FPGMLDELVAGGL  G+PCGENL KECEEEAGIPRSISNKA+ VGAVSYSDIKDYSYKRD
Sbjct: 244 FPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRD 303

Query: 301 VLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGC 352
           VLFCYDLKLPE+F+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG 
Sbjct: 304 VLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 363

[Close]

BLAST of MC06g0656 vs. NCBI nr
Match: KAG6587948.1 (Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577 bits (1486), Expect = 6.10e-205
Identity = 299/381 (78.48%), Postives = 316/381 (82.94%), Query Frame = 0


Query: 1   AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSV 60
           A  P IQPMACKSLA HRF N   IS I +ASMF R  +PT ST    VHRS TFA  SV
Sbjct: 2   ATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTAST----VHRSKTFACRSV 61

Query: 61  SVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGY 120
           SVASS+FTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+
Sbjct: 62  SVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGH 121

Query: 121 VHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPG 180
           VHHGFA HLKKYANVFTFP+DDSV+ GA L+L+ESL TPEDRT  VG+VVKCLGEE+I G
Sbjct: 122 VHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIQG 181

Query: 181 TRNE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQT 240
            RNE                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQT
Sbjct: 182 IRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241

Query: 241 FPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRD 300
           FPGMLDELVAGGL  GV C ENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRD
Sbjct: 242 FPGMLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRD 301

Query: 301 VLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGC 352
           VLFCYDLKLPESF+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG 
Sbjct: 302 VLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 361

[Close]

BLAST of MC06g0656 vs. ExPASy TrEMBL
Match: A0A6J1CUZ7 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014513 PE=4 SV=1)

HSP 1 Score: 686 bits (1769), Expect = 1.90e-248
Identity = 344/373 (92.23%), Postives = 344/373 (92.23%), Query Frame = 0


Query: 9   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 68
           MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT
Sbjct: 1   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 60

Query: 69  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 128
           WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH
Sbjct: 61  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 120

Query: 129 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNE---- 188
           LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNE    
Sbjct: 121 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNELYPV 180

Query: 189 -----------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 248
                                  VYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL
Sbjct: 181 TSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 240

Query: 249 VAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 308
           VAGGL  GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK
Sbjct: 241 VAGGLPQGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 300

Query: 309 LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY 352
           LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY
Sbjct: 301 LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY 360

[Close]

BLAST of MC06g0656 vs. ExPASy TrEMBL
Match: A0A6J1F0U6 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441109 PE=4 SV=1)

HSP 1 Score: 582 bits (1500), Expect = 2.18e-207
Identity = 300/381 (78.74%), Postives = 318/381 (83.46%), Query Frame = 0


Query: 1   AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSV 60
           A  P IQPMACKSLA HRF N   IS I +ASMF R R+PT ST    +HRS TFA  SV
Sbjct: 2   ATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTAST----LHRSKTFACRSV 61

Query: 61  SVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGY 120
           SVASS+FTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+
Sbjct: 62  SVASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGH 121

Query: 121 VHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPG 180
           VHHGFA HLKKYANVFTFP+DDSV+ GA L+L+ESL TPEDRT  VG+VVKCLGEE+IPG
Sbjct: 122 VHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPG 181

Query: 181 TRNE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQT 240
            RNE                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQT
Sbjct: 182 IRNEMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241

Query: 241 FPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRD 300
           FPGMLDELVAGGL  GV CGENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRD
Sbjct: 242 FPGMLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRD 301

Query: 301 VLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGC 352
           VLFCYDLKLPESF+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG 
Sbjct: 302 VLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 361

[Close]

BLAST of MC06g0656 vs. ExPASy TrEMBL
Match: A0A6J1KL70 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496248 PE=4 SV=1)

HSP 1 Score: 577 bits (1486), Expect = 2.95e-205
Identity = 296/381 (77.69%), Postives = 316/381 (82.94%), Query Frame = 0


Query: 1   AASPSIQPMACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSV 60
           A  P IQPMACKSLA H F N   IS I +ASMF R  +PT ST    VHRS TFA  SV
Sbjct: 2   ATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTAST----VHRSKTFACRSV 61

Query: 61  SVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGY 120
           S+ASS+FTWEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+QI+G+
Sbjct: 62  SIASSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGH 121

Query: 121 VHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPG 180
           VHHGFA HLKKYANVFTFP+DDSV+ GA L+L+ESL TPEDRT  VG+VVKCLGEE+IPG
Sbjct: 122 VHHGFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPG 181

Query: 181 TRNE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQT 240
            RNE                           VYGVQMNGYVE+DG+KFLWIAKRSQMKQT
Sbjct: 182 VRNEMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241

Query: 241 FPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRD 300
           FPGMLDELVAGGL  G+ CGENL KECEEEAGIPRSISNKAI VGAVSYSDIKDYSYKRD
Sbjct: 242 FPGMLDELVAGGLPEGISCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRD 301

Query: 301 VLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGC 352
           VLFCYDLKLPESF+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVII+FLFRHG 
Sbjct: 302 VLFCYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIEFLFRHGY 361

[Close]

BLAST of MC06g0656 vs. ExPASy TrEMBL
Match: A0A1S3CJI7 (nudix hydrolase 20, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501537 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 1.63e-194
Identity = 269/346 (77.75%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 9   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 68
           MA KSLA H F NF  +   C+AS   RT + T STFRPTVHRSI+ AS  VSV   SFT
Sbjct: 1   MASKSLA-HSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60

Query: 69  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 128
           W+D +H+S PESFQDDP+DLTGYFEKVKLCNRGSD QSEF PFVID QI+GYVHHGFA+H
Sbjct: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKH 120

Query: 129 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNEVYGV 188
           L++Y NVFTF QDDSV+ GA LTL+ESL TPEDRTR VGDVVKCLGEEVIPGTRNEVYGV
Sbjct: 121 LQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNEVYGV 180

Query: 189 QMNGYVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPR 248
            MNGYVE++G+KFLW+AKRSQ K TFPGMLD LVAGGL  G+ CGENL KEC+EEAGIPR
Sbjct: 181 HMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPR 240

Query: 249 SISNKAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVAN 308
           S+S +AI VGA+SY+DIK Y YKRDV FCYDLKLPESFVP+NQDGEVEGF LLPV NVAN
Sbjct: 241 SVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVAN 300

Query: 309 VIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTCF 352
           VIRRTQ FKPNCSLVII+FLFRHG IKPES GYLELLQSL+SGTCF
Sbjct: 301 VIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 345

[Close]

BLAST of MC06g0656 vs. ExPASy TrEMBL
Match: A0A6J1CSY3 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014470 PE=4 SV=1)

HSP 1 Score: 546 bits (1406), Expect = 7.11e-193
Identity = 284/379 (74.93%), Postives = 308/379 (81.27%), Query Frame = 0


Query: 6   IQPMACKSLAPH--RFCNFPAISFICDASMFLRTRFPTTSTFRPTV-HRSITFASGSVSV 65
           IQ MACKSLA H  RF NF  IS  C+AS   RT +P  STFRPTV HRSI+ AS SVSV
Sbjct: 14  IQFMACKSLASHSHRFRNFSTISSNCNASTISRTTYPMASTFRPTVVHRSISVASRSVSV 73

Query: 66  ASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVH 125
           ASS FTW+D L ISLPE +QDDPSDLTGYFEKVKLCNRGSDMQSEFLPFV+D+QI+GYVH
Sbjct: 74  ASS-FTWDDVLRISLPEIYQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVVDDQIVGYVH 133

Query: 126 HGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTR 185
           HGFAEHLK+YANVFTF  DDSVQ  AYLTL+ESL TPEDRT+ VGDVVKCLGEE+IPGTR
Sbjct: 134 HGFAEHLKQYANVFTFSGDDSVQFRAYLTLHESLKTPEDRTQAVGDVVKCLGEEIIPGTR 193

Query: 186 NE---------------------------VYGVQMNGYVERDGQKFLWIAKRSQMKQTFP 245
           NE                           VYGV MNGYVE++G+KFLW+AKRSQ KQTFP
Sbjct: 194 NELYPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKQTFP 253

Query: 246 GMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVL 305
           GMLD LVAGGL  G+PC ENL KECEEEA IPRS+SN+AI VGAVSYSDIK ++YKRDV 
Sbjct: 254 GMLDHLVAGGLPQGIPCVENLMKECEEEACIPRSLSNEAIPVGAVSYSDIKGHTYKRDVQ 313

Query: 306 FCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIK 352
           FCYDLKLPESF+P+NQDGEVEGF LLPV NVANVIRRTQLFKPNCSLVIIDFLFRHG I 
Sbjct: 314 FCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVIIDFLFRHGYIY 373

[Close]

BLAST of MC06g0656 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 362.5 bits (929), Expect = 3.9e-100
Identity = 187/343 (54.52%), Postives = 235/343 (68.51%), Query Frame = 0


Query: 41  TTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNR 100
           ++ +  P  H     A+ +V + SSSFTW+D +     E    + SDLTG+FEK+  CNR
Sbjct: 35  SSMSLSPLRHSRALSAATTVPI-SSSFTWDDVIETGRAEY---NSSDLTGFFEKINRCNR 94

Query: 101 GSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSV--QCGAYLTLNESLTT 160
           GS+   EF+PFVI+ QI+GY+H  F E+L+++ ++FTF Q+ S   +   Y+TLN  L  
Sbjct: 95  GSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQNGSCPDRVDGYVTLNLMLQK 154

Query: 161 PEDRTRGVGDVVKCLGEE-VIPGTRNEV---------------------------YGVQM 220
           PEDRTR V DV+K LG++ +IPG RNE+                           YGV M
Sbjct: 155 PEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVFFSLERAAAPYFGIKGYGVHM 214

Query: 221 NGYVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSI 280
           NGYVERDGQK LWI KRS  K T+PGMLD LVAGGL  G+ CG NL KECEEEAGI R+I
Sbjct: 215 NGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGISCGGNLVKECEEEAGISRAI 274

Query: 281 SNKAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVI 340
           +++AI+VGAVSY DI  Y +KRDVLFCYDL+LPE FVPKNQDGEVE F+L+PV  VA+VI
Sbjct: 275 ADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVI 334

Query: 341 RRTQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTC 352
           ++T  FK NCSLVIIDFLFRHG I+PES GYL+L Q L++  C
Sbjct: 335 KKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQRLRNRDC 373

[Close]

BLAST of MC06g0656 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-92
Identity = 174/341 (51.03%), Postives = 224/341 (65.69%), Query Frame = 0


Query: 41  TTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNR 100
           ++S+  P +H S   +  +V + S SFTW D    S  E    + SDLTG+ EKV  CNR
Sbjct: 30  SSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNR 89

Query: 101 GSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPE 160
           G +   EF+PFVI+ QI+GY+H GF ++L+ + ++FTF      Q G ++TLN  L  PE
Sbjct: 90  GLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPE 149

Query: 161 DRTRGVGDVVKCLGEE-VIPGTRNEV---------------------------YGVQMNG 220
           +RTR V  V+K LG + +IPG RNE+                           Y + +NG
Sbjct: 150 ERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNG 209

Query: 221 YVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGL--GVPCGENLRKECEEEAGIPRSISN 280
           YVERDGQKFLWI KRS  K T+PG LD LVAGGL  G+   ENL KECEEEAGI + +++
Sbjct: 210 YVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGLPHGISVCENLVKECEEEAGISKVLAD 269

Query: 281 KAISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRR 340
           +AI+VG VSY DI  Y + RDVLFCYDL+LP+ FVP NQDGEV+ FRL+PV  VANV+R+
Sbjct: 270 RAIAVGVVSYMDIDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRK 329

Query: 341 TQLFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTC 352
           T  FK +CSLVIIDFLFRHG I+PES GYL+L + L++G C
Sbjct: 330 TSFFKDSCSLVIIDFLFRHGLIRPESPGYLDLYRRLRNGDC 364

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BLAST of MC06g0656 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 332.0 bits (850), Expect = 5.7e-91
Identity = 172/339 (50.74%), Postives = 221/339 (65.19%), Query Frame = 0


Query: 41  TTSTFRPTVHRSITFASGSVSVASSSFTWEDALHISLPESFQDDPSDLTGYFEKVKLCNR 100
           ++S+  P +H S   +  +V + S SFTW D    S  E    + SDLTG+ EKV  CNR
Sbjct: 30  SSSSRSPPLHISRVLSVETVPL-SPSFTWNDVFENSRKEYVPQNSSDLTGFLEKVDRCNR 89

Query: 101 GSDMQSEFLPFVIDNQIIGYVHHGFAEHLKKYANVFTFPQDDSVQCGAYLTLNESLTTPE 160
           G +   EF+PFVI+ QI+GY+H GF ++L+ + ++FTF      Q G ++TLN  L  PE
Sbjct: 90  GLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFNDIFTFS-----QYGGHVTLNMMLDKPE 149

Query: 161 DRTRGVGDVVKCLGEE-VIPGTRNEV---------------------------YGVQMNG 220
           +RTR V  V+K LG + +IPG RNE+                           Y + +NG
Sbjct: 150 ERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLKGYAIHVNG 209

Query: 221 YVERDGQKFLWIAKRSQMKQTFPGMLDELVAGGLGVPCGENLRKECEEEAGIPRSISNKA 280
           YVERDGQKFLWI KRS  K T+PG LD LVAGGL      NL KECEEEAGI + ++++A
Sbjct: 210 YVERDGQKFLWIGKRSLAKSTYPGKLDHLVAGGL------NLVKECEEEAGISKVLADRA 269

Query: 281 ISVGAVSYSDIKDYSYKRDVLFCYDLKLPESFVPKNQDGEVEGFRLLPVENVANVIRRTQ 340
           I+VG VSY DI  Y + RDVLFCYDL+LP+ FVP NQDGEV+ FRL+PV  VANV+R+T 
Sbjct: 270 IAVGVVSYMDIDRYCFTRDVLFCYDLELPQDFVPTNQDGEVDSFRLIPVAQVANVVRKTS 329

Query: 341 LFKPNCSLVIIDFLFRHGCIKPESLGYLELLQSLKSGTC 352
            FK +CSLVIIDFLFRHG I+PES GYL+L + L++G C
Sbjct: 330 FFKDSCSLVIIDFLFRHGLIRPESPGYLDLYRRLRNGDC 356

The following BLAST results are available for this feature:

BLAST of MC06g0656 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VXZ0	5.6e-99	54.52	Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2...	[more]
P0C026	2.5e-91	51.03	Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2...	[more]
P47173	1.2e-29	42.86	Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
P41888	7.9e-21	39.19	Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843...	[more]

BLAST of MC06g0656 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144971.1	3.92e-248	92.23	nudix hydrolase 20, chloroplastic-like [Momordica charantia]	[more]
XP_022933802.1	4.50e-207	78.74	nudix hydrolase 20, chloroplastic-like [Cucurbita moschata]	[more]
XP_023530783.1	9.08e-207	79.37	nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02353078...	[more]
XP_038878620.1	1.56e-205	78.48	nudix hydrolase 20, chloroplastic-like [Benincasa hispida]	[more]
KAG6587948.1	6.10e-205	78.48	Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC06g0656 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CUZ7	1.90e-248	92.23	nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1F0U6	2.18e-207	78.74	nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KL70	2.95e-205	77.69	nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114962...	[more]
A0A1S3CJI7	1.63e-194	77.75	nudix hydrolase 20, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1CSY3	7.11e-193	74.93	nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of MC06g0656 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19460.1	3.9e-100	54.52	nudix hydrolase homolog 20	[more]
AT5G19470.1	1.8e-92	51.03	nudix hydrolase homolog 24	[more]
AT5G19470.2	5.7e-91	50.74	nudix hydrolase homolog 24	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031804	Domain of unknown function DUF4743	PFAM	PF15916	DUF4743	coord: 91..190 e-value: 4.0E-22 score: 78.4
IPR000086	NUDIX hydrolase domain	PFAM	PF00293	NUDIX	coord: 196..304 e-value: 1.7E-6 score: 28.1
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 161..324 score: 9.060937
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 183..348 e-value: 1.6E-57 score: 196.2
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 186..349 coord: 65..186
None	No IPR available	PANTHER	PTHR13622:SF10	SI:DKEY-6N6.2	coord: 186..349 coord: 65..186
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 195..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0656.1	MC06g0656.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0044715	8-oxo-dGDP phosphatase activity