MC06g0590 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0590 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 2.6e-178
Identity = 353/636 (55.50%), Postives = 430/636 (67.61%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNG 64
           ++RKAIG VKDQTSI IAKVA N AP+LEV +VKATSHD+D   D+YI EI++L S S G
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGF 124
           YV ACV ++S+RL KTRDWIVALKALM+VHR+L +G P F EEI+YATRRG R+LN+S F
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 RDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVR--GSISDGNSDESDYGRQRRRQ-- 184
           RDEAHS+SWDHS FVR+YA YLD  +E  + E++ R  G  S  +    D G  R R   
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 ----------------------------------------SPWRSMRPERVLDEFDQLLR 244
                                                   +P R M PER+  +   L R
Sbjct: 184 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQR 243

Query: 245 MLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICEALGVLLDHFKEMEYGDCLRA 304
           +LDR L+CRP G+AK+SR+++ A++  VKESF LY +ICE L VLLD F +MEY DC++A
Sbjct: 244 LLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKA 303

Query: 305 FDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITHNLLGTLGDFLKEMGRRPKDF 364
           FD Y +AAK+ID+L+ FY WCKD G+ARSSEYPEVQRIT  LL TL +F+++  +R K  
Sbjct: 304 FDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSP 363

Query: 365 NERRRE-EKLKLPPADDPEQDMNEMKALPPPKNH---DPSLHSVLPQTRLPKEDLVNLRE 424
             +  E      PP ++P  DMNE+KALPPP+NH    P      PQ     +DLVNLRE
Sbjct: 364 ERKEIEAPPAPAPPVEEP-VDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKADWELAL 484
           D+VS D+QGNK ALALF+GPP  N  G WE FSS  D  V+SAW+ PAAE GKADWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPANN--GKWEAFSS--DNNVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVN-AEQFGGSSSSVALQVQGKTVAA 544
           VETASNL  QKA MGGG DPLLLN MYDQG +R+HV+ +E  GGSSSSVAL + GK V +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGK-VNS 543

Query: 545 QVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREGM 585
            +LALPAPDGTV  V ++DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ +WQQY +EGM
Sbjct: 544 HILALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of MC06g0590 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 619.4 bits (1596), Expect = 4.2e-176
Identity = 350/657 (53.27%), Postives = 439/657 (66.82%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNG 64
           +IRKAIGAVKDQTSI IAKVA N AP+LEV +VKATSHD+DP  ++YI EI+NL S S G
Sbjct: 4   SIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSLSRG 63

Query: 65  YVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGF 124
           Y+ ACV ++S+RL+KTRDW+VALKALM+VHR+L +G P F EEI+Y+TRRG R+LN+S F
Sbjct: 64  YILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSDF 123

Query: 125 RDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDG------NSDESDYGR--- 184
           RDEAHS+SWDHS FVR+YA YLD  +E  + E+K   S++ G      ++++  YGR   
Sbjct: 124 RDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYGRGRD 183

Query: 185 -------------------------------------------------------QRRRQ 244
                                                                  +++  
Sbjct: 184 DFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKKVV 243

Query: 245 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 304
           +P R M PER+  +   L R+LDR L+ RP G+AK+SR+++ A++  V+ESF LY +ICE
Sbjct: 244 TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADICE 303

Query: 305 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 364
            L VLLD F +MEY DC++AFD Y +AAK+ID+L+ FY WCK+ G+ARSSEYPEVQRIT 
Sbjct: 304 VLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRITS 363

Query: 365 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNH-DPSLHS 424
            LL TL +F+++  +R K    +  E    +   ++PE DMNE+KALPPP+N+  P    
Sbjct: 364 KLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPE 423

Query: 425 VLPQTRLPK--EDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 484
             PQ   P+  EDLVNLREDEV+AD+QGNK ALALF+GPP  N  G WE FSSN    V+
Sbjct: 424 PEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGNN--GKWEAFSSN---GVT 483

Query: 485 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 544
           SAW+ PAAE GKADWELALVET SNL KQ A +GGGFD LLLN MYDQG +R+HV+  Q 
Sbjct: 484 SAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQL 543

Query: 545 -GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKK 588
            GGS+SSVAL + GKT   QVLALPAPDGTV  V ++DPFAASLT+PPPSYVQ+AEMEKK
Sbjct: 544 TGGSASSVALPLPGKT-NNQVLALPAPDGTVEKV-NQDPFAASLTIPPPSYVQMAEMEKK 603

BLAST of MC06g0590 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-116
Identity = 243/603 (40.30%), Postives = 370/603 (61.36%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTA--PELEVLVVKATSHDEDPDDDRYIIEIVNL 60
           M  S  ++AIGAVKDQTS+ +AKV G +A   EL+V +VKAT H+E P +++YI EI++L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  CSHSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRV 120
            S+S  Y++ACV T+S+RLNKT+ W VALK L+++ R+L +G  ++ +EI +ATRRG R+
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSI--SDGNSDESDYGRQ 180
           LN+S FRD + SNSWD+S FVR+YALYLD  ++  +  +  +  +    G +DE +  + 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQA 180

Query: 181 RR--------RQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAV 240
                     R  P   M+ E++      L ++LDR LACRP G A+++R+VI A++  V
Sbjct: 181 AADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIV 240

Query: 241 KESFGLYIEICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIAR 300
           KESF +Y ++ E +G+L++ F E++  D ++ +D++   +K+ ++L +FY+WCK+MGIAR
Sbjct: 241 KESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIAR 300

Query: 301 SSEYPEVQRITHNLLGTLGDFLK-----EMGRRPKDFNERRRE--EKLKLPPADDPEQDM 360
           SSEYPE+++IT   L  + +F++     E  ++ K       E  ++ +    ++ ++DM
Sbjct: 301 SSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDM 360

Query: 361 NEMKAL--PPPKNHDPSLHSVLPQTRLPKEDLVNLREDEV------------SADEQGNK 420
           N +KAL  PPPK  D     V P+    +E ++  +++E+             A + G+ 
Sbjct: 361 NAIKALPEPPPKEED----DVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDS 420

Query: 421 LALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKADWELALVETASNLSKQK 480
           LALALF GP           ++S    E    W+  A +   ADWE ALV+TA+NLS QK
Sbjct: 421 LALALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQK 480

Query: 481 ADMGGGFDPLLLNAMYDQGTIRRHV---NAEQFGGSSSSVALQVQGKTVAAQVLALPAPD 540
           +++GGGFD LLLN MY  G +   V    A    GS+SS+A    G+  AA +LALPAP 
Sbjct: 481 SELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRP-AATMLALPAPS 540

Query: 541 ------GTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREGMHGQ 562
                 G +      DPFAASL V PP+YVQ+ +MEKKQ++L++EQ++W QY R+G  G 
Sbjct: 541 TANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGH 585

BLAST of MC06g0590 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 6.4e-116
Identity = 248/609 (40.72%), Postives = 363/609 (59.61%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTA--PELEVLVVKATSHDEDPDDDRYIIEIVNL 60
           M  S +++AIGAVKDQTS+ +AKV G ++   ELE+ VVKAT HD+ P +D+YI EI+ L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  CSHSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRV 120
            S+S  YVSACV T+S+RLNKT++W VALK L+++ R+L DG  ++ +EI +ATRRG R+
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLV-----LEKKVRGSISDGNSDESDY 180
           LN+S FRD + S+SWD+S FVR+YALYLD  +++ +      +K   G   DG+S E D 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDD 180

Query: 181 GRQRR----------RQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAA 240
            R             +  P   M+ E++ +    L ++LDR LACRP G AK++R+VI A
Sbjct: 181 HRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVA 240

Query: 241 VHLAVKESFGLYIEICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKD 300
           ++  VKESF LY  I E +GVL++ F E++  D ++ ++++   +K+ D+L  FY WCK+
Sbjct: 241 MYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKN 300

Query: 301 MGIARSSEYPEVQRITHNLLGTLGDFLKEMG-------RRPKDFNERRREEKLKLPPADD 360
           M +ARSSEYPE+++IT   L  + +F+++         +     + +  EE+ K     +
Sbjct: 301 MAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQE 360

Query: 361 PEQDMNEMKALPPPKNHDPSLHSVLPQTRLPKEDLVNLREDE-----------VSADEQG 420
            ++D+N +KALP P+  +        +T+   E++V+ ++ E           V+A   G
Sbjct: 361 NQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVG 420

Query: 421 NKLALALFSGPPITNPDG--SWETFSSNQDPEVSSAWKTPAAEAGKADWELALVETASNL 480
           + LALALF G   T       WE F+ N                  ADWE  LV +A+ L
Sbjct: 421 DSLALALFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATRL 480

Query: 481 SKQKADMGGGFDPLLLNAMYDQGTIRRHV---NAEQFGGSSSSVALQVQGKTVAAQVLAL 540
           S QK+++GGGFD LLL+ MY  G +   V    A    GS+SSVA    G + AA +LAL
Sbjct: 481 SGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAG-SPAASMLAL 540

Query: 541 PAPDGTVTPVGHR------DPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREG 564
           PAP  T    G+R      DPFAASL V PP+YVQ+ +MEKKQ+LL++EQ++W QY R G
Sbjct: 541 PAPPPTAN--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSG 589

BLAST of MC06g0590 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.6e-66
Identity = 137/331 (41.39%), Postives = 209/331 (63.14%), Query Frame = 0

Query: 4   STIRKAIGAVKDQTSISIAKVA-----GNTAPELEVLVVKATSHDED-PDDDRYIIEIVN 63
           S ++KAIGAVKDQTSIS+AKVA     G     LEV ++KATSHDE+ P DDR + EI+ 
Sbjct: 3   SKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEILG 62

Query: 64  LCSHSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMR 123
           + S    + ++C   I +R+ +TR+WIVALK+L++V R+  DG P F  E+++A +RG +
Sbjct: 63  IISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGAK 122

Query: 124 VLNLSGFRDEAHSNSWDHSGFVRSYALYLD--------GMIEHLVLEKKVRGSISDGNSD 183
           +LNLS FRD+++S  WD + FVR++ALYLD        G ++     ++  G IS  ++ 
Sbjct: 123 ILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNSTT 182

Query: 184 ESDYGRQ---RRRQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHL 243
            S +  +   +  +   R M+P  +LD+     ++LDR +A RP G AK++RLV  +++ 
Sbjct: 183 RSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSLYA 242

Query: 244 AVKESFGLYIEICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGI 303
            ++ESF LY +I + L +LLD F  ++Y  C+ AF   V A+K+ ++L  FY   K +GI
Sbjct: 243 VMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSIGI 302

Query: 304 ARSSEYPEVQRITHNLLGTLGDFLKEMGRRP 318
            R+SEYP +Q+I+  LL TL +FLK+    P
Sbjct: 303 GRTSEYPSIQKISLELLETLQEFLKDQSSFP 333

BLAST of MC06g0590 vs. NCBI nr
Match: XP_022145098.1 (probable clathrin assembly protein At4g32285 [Momordica charantia])

HSP 1 Score: 1154 bits (2986), Expect = 0.0
Identity = 586/587 (99.83%), Postives = 586/587 (99.83%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS
Sbjct: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
           HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN
Sbjct: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ 180
           LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ
Sbjct: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ 180

Query: 181 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 240
           SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE
Sbjct: 181 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 240

Query: 241 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 300
           ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH
Sbjct: 241 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 300

Query: 301 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV 360
           NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV
Sbjct: 301 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV 360

Query: 361 LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW 420
           LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW
Sbjct: 361 LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW 420

Query: 421 KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS 480
           KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS
Sbjct: 421 KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS 480

Query: 481 SSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL 540
           SSSVALQVQGKT AAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL
Sbjct: 481 SSSVALQVQGKTXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL 540

Query: 541 VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF 587
           VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF
Sbjct: 541 VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF 587

BLAST of MC06g0590 vs. NCBI nr
Match: XP_023529315.1 (putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 471/593 (79.43%), Postives = 515/593 (86.85%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKA+GAVKDQTSISIAKVAGNTA ELEVLVVKATSHDEDP D RYI EIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
            SNGYVSACV+T+SKRL+KTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATR+G+RVL+
Sbjct: 61  TSNGYVSACVITVSKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLD 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGN---SDESDYGRQR 180
           LSGFRDEAHSNSWDHS FVRSYALYLD M E+LVLEK++RGS  D N    DE D  RQR
Sbjct: 121 LSGFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGS-DDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIE 240
           RRQSPWRSMRPE V+DE  QLLR+LDRVLACRP GMAKSSRLVI A+ L V +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRVLACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQR 300
           ICE LG LLD FKEMEYGDCLRAFDV  NAAKKID+L EFY WCKD+GIARSSEYPE QR
Sbjct: 241 ICEGLGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSL 360
           IT NLL TLG FLKEM RRPKD+NERRREEK+K  PA++PE  MNE+KALPP +N  P+L
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 HSVLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 420
               P +R    DLVNLRED VSADEQGNKLALALFSGPP  NP+GSWETFSS++ PEVS
Sbjct: 361 Q---PPSR--PIDLVNLREDGVSADEQGNKLALALFSGPPA-NPNGSWETFSSDKQPEVS 420

Query: 421 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 480
           SAW+TPAAEAGK +WELALVETASNLSKQKADMGGGFDPLLLN MYD GTIR+H NAEQF
Sbjct: 421 SAWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRKHANAEQF 480

Query: 481 GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQ 540
           GGSSSSVAL+ QGKT AAQVLALPAPDGTV P+G +DPFAASLTVPPPSYVQIAEME KQ
Sbjct: 481 GGSSSSVALKGQGKT-AAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEMKQ 540

Query: 541 QLLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           QLL+QEQL+WQQYGR+GMHGQVGL K+ GGSA++      I P +SAY+YAPF
Sbjct: 541 QLLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGPNPQPIAPQSSAYHYAPF 585

BLAST of MC06g0590 vs. NCBI nr
Match: XP_022934011.1 (probable clathrin assembly protein At4g32285 [Cucurbita moschata])

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 470/593 (79.26%), Postives = 515/593 (86.85%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKA+GAVKDQTSISIAKVAGNTA ELEVLVVKATSHDEDP D RYI EIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
            SNGYVSACV+TISKRL+KTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATR+G+RVL+
Sbjct: 61  TSNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLD 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGN---SDESDYGRQR 180
           LS FRDEAHSNSWDHS FVRSYALYLD M E+LVLEK++RGS  D N    DE D  RQR
Sbjct: 121 LSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGS-DDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIE 240
           RRQSPWRSMRPE V+DE  QLLR+LDR+LACRP GMAKSSRLVI A+ L V +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQR 300
           ICEALG LLD FKEMEYGDCLRAFDV  NAAKKID+L EFY WCKD+GIARSSEYPE QR
Sbjct: 241 ICEALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSL 360
           IT NLL TLG FLKEM RRPKD+NERRREEK+K  PA++PE  MNE+KALPP +N  P+L
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 HSVLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 420
               P +R   EDLVNLRED VSADEQGNKLALALFSGPP  NP+GSWETFSS++ PEVS
Sbjct: 361 Q---PPSR--PEDLVNLREDGVSADEQGNKLALALFSGPPA-NPNGSWETFSSDKQPEVS 420

Query: 421 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 480
           SAW+TPAAEAGK +WELALVETASNLSKQKADMGGGFDPLLLN MYD GTIR+H NAEQF
Sbjct: 421 SAWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQF 480

Query: 481 GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQ 540
            GSSSSVA++ Q KT AAQVLALPAPDGTV P+G +DPFAASLTVPPPSYVQIAEMEKKQ
Sbjct: 481 DGSSSSVAMKGQSKT-AAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQ 540

Query: 541 QLLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           QLL+QEQL+WQQYGR+GMHGQVGL K+ GGSA++      I P +SAY+YAPF
Sbjct: 541 QLLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of MC06g0590 vs. NCBI nr
Match: KAG6587890.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880 bits (2274), Expect = 0.0
Identity = 469/593 (79.09%), Postives = 514/593 (86.68%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKA+GAVKDQTSISIAKVAGNTA ELEVLVVKATSHDEDP   RYI EIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPAGHRYIREIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
            SNGYVSACV+TISKRL+KTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATR+G+RVL+
Sbjct: 61  TSNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLD 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGN---SDESDYGRQR 180
           LS FRDEAHSNSWDHS FVRSYALYLD M E+LVLEK++RGS  D N    DE D  RQR
Sbjct: 121 LSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGS-DDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIE 240
           RRQSPWRSMRPE V+DE  QLLR+LDR+LACRP GMAKSSRLVI A+ L V +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQR 300
           ICEALG LLD FKEMEYGDCLRAFDV  NAAKKID+L EFY WC D+GIARSSEYPE QR
Sbjct: 241 ICEALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCTDVGIARSSEYPEAQR 300

Query: 301 ITHNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSL 360
           IT NLL TLG FLKEM RRPKD+NERRREEK+K  PA++PE  MNE+KALPP +N  P+L
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 HSVLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 420
               P +R   EDLVNLRED VSADEQGNKLALALFSGPP  NP+GSWETFSS++ PEVS
Sbjct: 361 Q---PPSR--PEDLVNLREDGVSADEQGNKLALALFSGPPA-NPNGSWETFSSDKQPEVS 420

Query: 421 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 480
           SAW+TPAAEAGK +WELALVETASNLSKQKADMGGGFDPLLLN MYD GTIR+H NAEQF
Sbjct: 421 SAWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQF 480

Query: 481 GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQ 540
            GSSSSVA++ QGKT AAQVLALPAPDGTV P+G +DPFAASLTVPPPSYVQIAEMEKKQ
Sbjct: 481 DGSSSSVAMKGQGKT-AAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQ 540

Query: 541 QLLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           QLL+QEQL+WQQYGR+GMHGQVGL K+ GGSA++      I P +SAY+YAPF
Sbjct: 541 QLLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of MC06g0590 vs. NCBI nr
Match: XP_038879765.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 873 bits (2255), Expect = 1.05e-314
Identity = 456/592 (77.03%), Postives = 515/592 (86.99%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKAIG +KDQT+ISIAKVAGN  PEL+VLVVKATSHDEDP DDRYI EIVNLCS
Sbjct: 1   MAQSTIRKAIGVMKDQTTISIAKVAGNMTPELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  -HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVL 120
            +SNGYV+ACVVTIS+RL+KTRDWIVALK+L+VVHR+LIDGHPSFGEEIVYATR+GMRVL
Sbjct: 61  NYSNGYVNACVVTISRRLSKTRDWIVALKSLIVVHRILIDGHPSFGEEIVYATRKGMRVL 120

Query: 121 NLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRR 180
           NLSGFRDEAHSNSWDHS F+R YALYLD +IE+LVLEK+++G       DES Y  + R+
Sbjct: 121 NLSGFRDEAHSNSWDHSSFLRFYALYLDELIENLVLEKRLKGG------DESCY--RSRK 180

Query: 181 QSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEIC 240
           QSPWRSMRPERV++E +QLLRMLDRVLACRP GMAK+SRLVI A++L +KESFGLY+EIC
Sbjct: 181 QSPWRSMRPERVVEELNQLLRMLDRVLACRPAGMAKNSRLVITALNLIIKESFGLYVEIC 240

Query: 241 EALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRIT 300
           +ALGVLLDHFKEMEY DCLRAFDV  NAAK I+ L+EFY WCKD+GIARSSEYPEVQ IT
Sbjct: 241 DALGVLLDHFKEMEYVDCLRAFDVCANAAKNINDLVEFYGWCKDVGIARSSEYPEVQMIT 300

Query: 301 HNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHS 360
           HNLL TLG FLKEM RRPKDF+E R EE++K  P ++   DMNE+KALP  +N  P   S
Sbjct: 301 HNLLITLGGFLKEMSRRPKDFDEMRTEEEMKPLPVNESGYDMNEIKALPLLENCAPVPQS 360

Query: 361 VLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSA 420
           +L +T+ PKEDLVNLRED VSADEQGNKLALALFSGPPITNP+GSWETFSSN +PEVSSA
Sbjct: 361 IL-RTQSPKEDLVNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPEVSSA 420

Query: 421 WKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGG 480
           W+TPAAE GK DWEL LVETAS+LSKQK DMGG FDPLLLN MYDQ T+RRHVNA QFGG
Sbjct: 421 WQTPAAEFGKPDWELTLVETASHLSKQKPDMGGCFDPLLLNGMYDQETVRRHVNAVQFGG 480

Query: 481 SSSSVALQVQGK-TVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           SSSSVA+ +QG+   AAQVLALPAPDGT  P+G+RDPFAASLTVPPPSYVQIAEMEKKQ+
Sbjct: 481 SSSSVAVALQGQGNAAAQVLALPAPDGTAEPIGYRDPFAASLTVPPPSYVQIAEMEKKQE 540

Query: 541 LLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           LL QEQL+W+QYGR+GM GQ GL K+GGGSA+V++    IMPH SAYYYA F
Sbjct: 541 LLTQEQLLWKQYGRDGMQGQGGLGKLGGGSAYVDLNPQQIMPHGSAYYYASF 583

BLAST of MC06g0590 vs. ExPASy TrEMBL
Match: A0A6J1CV16 (probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=LOC111014605 PE=4 SV=1)

HSP 1 Score: 1154 bits (2986), Expect = 0.0
Identity = 586/587 (99.83%), Postives = 586/587 (99.83%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS
Sbjct: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
           HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN
Sbjct: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ 180
           LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ
Sbjct: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRRQ 180

Query: 181 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 240
           SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE
Sbjct: 181 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 240

Query: 241 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 300
           ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH
Sbjct: 241 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 300

Query: 301 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV 360
           NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV
Sbjct: 301 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHSV 360

Query: 361 LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW 420
           LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW
Sbjct: 361 LPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAW 420

Query: 421 KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS 480
           KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS
Sbjct: 421 KTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGS 480

Query: 481 SSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL 540
           SSSVALQVQGKT AAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL
Sbjct: 481 SSSVALQVQGKTXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLL 540

Query: 541 VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF 587
           VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF
Sbjct: 541 VQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNVAIMPHASAYYYAPF 587

BLAST of MC06g0590 vs. ExPASy TrEMBL
Match: A0A6J1F0M3 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111441244 PE=4 SV=1)

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 470/593 (79.26%), Postives = 515/593 (86.85%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKA+GAVKDQTSISIAKVAGNTA ELEVLVVKATSHDEDP D RYI EIVNLCS
Sbjct: 1   MAQSTIRKALGAVKDQTSISIAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNLCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
            SNGYVSACV+TISKRL+KTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATR+G+RVL+
Sbjct: 61  TSNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLD 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGN---SDESDYGRQR 180
           LS FRDEAHSNSWDHS FVRSYALYLD M E+LVLEK++RGS  D N    DE D  RQR
Sbjct: 121 LSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRIRGS-DDTNFDRRDEFDNVRQR 180

Query: 181 RRQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIE 240
           RRQSPWRSMRPE V+DE  QLLR+LDR+LACRP GMAKSSRLVI A+ L V +SFGLY+E
Sbjct: 181 RRQSPWRSMRPESVVDELSQLLRILDRILACRPTGMAKSSRLVITALDLVVNDSFGLYVE 240

Query: 241 ICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQR 300
           ICEALG LLD FKEMEYGDCLRAFDV  NAAKKID+L EFY WCKD+GIARSSEYPE QR
Sbjct: 241 ICEALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEYPEAQR 300

Query: 301 ITHNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSL 360
           IT NLL TLG FLKEM RRPKD+NERRREEK+K  PA++PE  MNE+KALPP +N  P+L
Sbjct: 301 ITCNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPAEEPECYMNEIKALPPLENCSPTL 360

Query: 361 HSVLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 420
               P +R   EDLVNLRED VSADEQGNKLALALFSGPP  NP+GSWETFSS++ PEVS
Sbjct: 361 Q---PPSR--PEDLVNLREDGVSADEQGNKLALALFSGPPA-NPNGSWETFSSDKQPEVS 420

Query: 421 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 480
           SAW+TPAAEAGK +WELALVETASNLSKQKADMGGGFDPLLLN MYD GTIR+H NAEQF
Sbjct: 421 SAWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQF 480

Query: 481 GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQ 540
            GSSSSVA++ Q KT AAQVLALPAPDGTV P+G +DPFAASLTVPPPSYVQIAEMEKKQ
Sbjct: 481 DGSSSSVAMKGQSKT-AAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQ 540

Query: 541 QLLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           QLL+QEQL+WQQYGR+GMHGQVGL K+ GGSA++      I P +SAY+YAPF
Sbjct: 541 QLLMQEQLLWQQYGRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

BLAST of MC06g0590 vs. ExPASy TrEMBL
Match: A0A6J1I3H3 (probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC111470668 PE=4 SV=1)

HSP 1 Score: 857 bits (2215), Expect = 5.86e-309
Identity = 458/592 (77.36%), Postives = 505/592 (85.30%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKA+GAVKDQTSIS+AKVAGNTA ELEVLVVKATSHDEDP D RYI EIVN CS
Sbjct: 1   MAQSTIRKALGAVKDQTSISMAKVAGNTASELEVLVVKATSHDEDPADHRYIREIVNWCS 60

Query: 61  HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLN 120
            SNGYVSACV+TISKRL+KTRDWIVALKAL+VVHRVLIDG+ SFGEEIVYATR+G+RVL+
Sbjct: 61  TSNGYVSACVITISKRLSKTRDWIVALKALVVVHRVLIDGNSSFGEEIVYATRKGIRVLD 120

Query: 121 LSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISD--GNSDESDYGRQRR 180
           LS FRDEAHSNSWDHS FVRSYALYLD M E+LVLEK++RGS        DE D  RQ  
Sbjct: 121 LSSFRDEAHSNSWDHSSFVRSYALYLDEMAENLVLEKRMRGSDDTYFDRRDEFDNVRQ-- 180

Query: 181 RQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEI 240
             SPWRSMRPE V+DE  QLLR+LDR+LACRP GMAKSSRLVI+A+ L V +SFGLY+EI
Sbjct: 181 --SPWRSMRPESVVDELSQLLRILDRLLACRPTGMAKSSRLVISALDLVVNDSFGLYVEI 240

Query: 241 CEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRI 300
           C+ALG LLD FKEMEYGDCLRAFDV  NAAKKID+L EFY WCKD+GIARSSE+PE QRI
Sbjct: 241 CKALGYLLDRFKEMEYGDCLRAFDVCANAAKKIDELDEFYTWCKDVGIARSSEFPEAQRI 300

Query: 301 THNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLH 360
           T NLL TLG FLKEM RRPKD+NERRREEK+K  P ++PE  MNE+KALPP +N  P+L 
Sbjct: 301 TSNLLVTLGGFLKEMSRRPKDYNERRREEKVKSLPTEEPECYMNEIKALPPLENCSPTLQ 360

Query: 361 SVLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSS 420
              P +R   EDLVNLRED VSADEQGNKLA ALFSGPP  NP+GSWETFSS++ PEVSS
Sbjct: 361 ---PPSR--PEDLVNLREDGVSADEQGNKLAWALFSGPPA-NPNGSWETFSSDKQPEVSS 420

Query: 421 AWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFG 480
           AW+TPAAEAGK +WELALVETASNLSKQKADMGGGFDPLLLN MYD GTIR+H N EQFG
Sbjct: 421 AWQTPAAEAGKPEWELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANGEQFG 480

Query: 481 GSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           GSSSSVAL+ QGKT AAQVLALPAPDGTV PVG +DPFA SLTVPPPSYVQIAEMEKKQQ
Sbjct: 481 GSSSSVALKGQGKT-AAQVLALPAPDGTVKPVGVQDPFAVSLTVPPPSYVQIAEMEKKQQ 540

Query: 541 LLVQEQLVWQQYGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587
           LL+QEQL+WQQYG +GMHGQVGL K+ GGS ++      I P +SAY+YAPF
Sbjct: 541 LLMQEQLLWQQYGIDGMHGQVGLVKLAGGSVYIGPNPQPIAPQSSAYHYAPF 581

BLAST of MC06g0590 vs. ExPASy TrEMBL
Match: A0A5D3C4X9 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00870 PE=4 SV=1)

HSP 1 Score: 809 bits (2090), Expect = 2.88e-290
Identity = 430/572 (75.17%), Postives = 482/572 (84.27%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKAIGA+KDQT+ISIAKVAGN A EL+VLVVKATSHDEDP DDRYI EIVNLCS
Sbjct: 1   MAQSTIRKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  -HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVL 120
            +SNGYV+ACVVTISKRL+KTRDWIVALKAL+VVHRVLIDGHPSFGEEIVYATR+G RVL
Sbjct: 61  NYSNGYVNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVL 120

Query: 121 NLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRR 180
           +LSGFRDEAHSNSWDHS F+R YALYLD +IE LVLEKKV+G       DES Y  + +R
Sbjct: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGG------DESYY--RGKR 180

Query: 181 QSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEIC 240
           QS WRS+RPERV++E +QL+RMLDRVLACRP GMAKSSRLVI A++L +KESF LYIEIC
Sbjct: 181 QSSWRSIRPERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEIC 240

Query: 241 EALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRIT 300
           +ALGVLLD FKEMEY DCLR FDV  NA K ID+L+EFY WCKD+GIARSSEYP V+ IT
Sbjct: 241 DALGVLLDQFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMIT 300

Query: 301 HNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHS 360
           +NLL TLG FLKEM RRPKD+NE R +E +K     +   DMNE+KALPPP+N  P   S
Sbjct: 301 NNLLITLGGFLKEMTRRPKDYNEMRTKE-MKPVSVKESRYDMNEIKALPPPENCAPVSQS 360

Query: 361 VLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSA 420
           VL      KEDLVNLREDEVSADEQG KLA+A FSGPP TNP+GSWETFS N +PEVSS 
Sbjct: 361 VLQSL---KEDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSV 420

Query: 421 WKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGG 480
           W+T AAE GK DWELALVETAS+LSKQ+ DMGG FDP+LLN MYDQ  +RRHVN EQ GG
Sbjct: 421 WQTRAAEFGKPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGG 480

Query: 481 SSSSVALQVQGK-TVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           SSSSVA+ +QG+ +  +QVLALPAPDGTV  VG RDPFAASL VPPPSYVQIAEMEKKQQ
Sbjct: 481 SSSSVAVALQGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQ 540

Query: 541 LLVQEQLVWQQYGREGMHGQVGLAKIGGGSAH 570
           LL QEQL+W+QYGR+GM GQVGL K+G GSA+
Sbjct: 541 LLAQEQLLWKQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of MC06g0590 vs. ExPASy TrEMBL
Match: A0A1S3B992 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103487428 PE=4 SV=1)

HSP 1 Score: 809 bits (2090), Expect = 2.88e-290
Identity = 430/572 (75.17%), Postives = 482/572 (84.27%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCS 60
           MAQSTIRKAIGA+KDQT+ISIAKVAGN A EL+VLVVKATSHDEDP DDRYI EIVNLCS
Sbjct: 1   MAQSTIRKAIGAMKDQTTISIAKVAGNMARELDVLVVKATSHDEDPADDRYIREIVNLCS 60

Query: 61  -HSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVL 120
            +SNGYV+ACVVTISKRL+KTRDWIVALKAL+VVHRVLIDGHPSFGEEIVYATR+G RVL
Sbjct: 61  NYSNGYVNACVVTISKRLSKTRDWIVALKALIVVHRVLIDGHPSFGEEIVYATRKGTRVL 120

Query: 121 NLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDGNSDESDYGRQRRR 180
           +LSGFRDEAHSNSWDHS F+R YALYLD +IE LVLEKKV+G       DES Y  + +R
Sbjct: 121 DLSGFRDEAHSNSWDHSSFLRFYALYLDELIETLVLEKKVKGG------DESYY--RGKR 180

Query: 181 QSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEIC 240
           QS WRS+RPERV++E +QL+RMLDRVLACRP GMAKSSRLVI A++L +KESF LYIEIC
Sbjct: 181 QSSWRSIRPERVVEELNQLIRMLDRVLACRPNGMAKSSRLVITALNLVIKESFRLYIEIC 240

Query: 241 EALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRIT 300
           +ALGVLLD FKEMEY DCLR FDV  NA K ID+L+EFY WCKD+GIARSSEYP V+ IT
Sbjct: 241 DALGVLLDQFKEMEYADCLRVFDVCANAVKNIDELVEFYCWCKDVGIARSSEYPGVRMIT 300

Query: 301 HNLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNHDPSLHS 360
           +NLL TLG FLKEM RRPKD+NE R +E +K     +   DMNE+KALPPP+N  P   S
Sbjct: 301 NNLLITLGGFLKEMTRRPKDYNEMRTKE-MKPVSVKESRYDMNEIKALPPPENCAPVSQS 360

Query: 361 VLPQTRLPKEDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSA 420
           VL      KEDLVNLREDEVSADEQG KLA+A FSGPP TNP+GSWETFS N +PEVSS 
Sbjct: 361 VLQSL---KEDLVNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSV 420

Query: 421 WKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGG 480
           W+T AAE GK DWELALVETAS+LSKQ+ DMGG FDP+LLN MYDQ  +RRHVN EQ GG
Sbjct: 421 WQTRAAEFGKPDWELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGG 480

Query: 481 SSSSVALQVQGK-TVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQ 540
           SSSSVA+ +QG+ +  +QVLALPAPDGTV  VG RDPFAASL VPPPSYVQIAEMEKKQQ
Sbjct: 481 SSSSVAVALQGQGSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQ 540

Query: 541 LLVQEQLVWQQYGREGMHGQVGLAKIGGGSAH 570
           LL QEQL+W+QYGR+GM GQVGL K+G GSA+
Sbjct: 541 LLAQEQLLWKQYGRDGMQGQVGLEKVGNGSAY 560

BLAST of MC06g0590 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 626.7 bits (1615), Expect = 1.9e-179
Identity = 353/636 (55.50%), Postives = 430/636 (67.61%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNG 64
           ++RKAIG VKDQTSI IAKVA N AP+LEV +VKATSHD+D   D+YI EI++L S S G
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGF 124
           YV ACV ++S+RL KTRDWIVALKALM+VHR+L +G P F EEI+YATRRG R+LN+S F
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 RDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVR--GSISDGNSDESDYGRQRRRQ-- 184
           RDEAHS+SWDHS FVR+YA YLD  +E  + E++ R  G  S  +    D G  R R   
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 ----------------------------------------SPWRSMRPERVLDEFDQLLR 244
                                                   +P R M PER+  +   L R
Sbjct: 184 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQR 243

Query: 245 MLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICEALGVLLDHFKEMEYGDCLRA 304
           +LDR L+CRP G+AK+SR+++ A++  VKESF LY +ICE L VLLD F +MEY DC++A
Sbjct: 244 LLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKA 303

Query: 305 FDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITHNLLGTLGDFLKEMGRRPKDF 364
           FD Y +AAK+ID+L+ FY WCKD G+ARSSEYPEVQRIT  LL TL +F+++  +R K  
Sbjct: 304 FDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSP 363

Query: 365 NERRRE-EKLKLPPADDPEQDMNEMKALPPPKNH---DPSLHSVLPQTRLPKEDLVNLRE 424
             +  E      PP ++P  DMNE+KALPPP+NH    P      PQ     +DLVNLRE
Sbjct: 364 ERKEIEAPPAPAPPVEEP-VDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKADWELAL 484
           D+VS D+QGNK ALALF+GPP  N  G WE FSS  D  V+SAW+ PAAE GKADWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPANN--GKWEAFSS--DNNVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVN-AEQFGGSSSSVALQVQGKTVAA 544
           VETASNL  QKA MGGG DPLLLN MYDQG +R+HV+ +E  GGSSSSVAL + GK V +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGK-VNS 543

Query: 545 QVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREGM 585
            +LALPAPDGTV  V ++DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ +WQQY +EGM
Sbjct: 544 HILALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of MC06g0590 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 626.7 bits (1615), Expect = 1.9e-179
Identity = 353/636 (55.50%), Postives = 430/636 (67.61%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNG 64
           ++RKAIG VKDQTSI IAKVA N AP+LEV +VKATSHD+D   D+YI EI++L S S G
Sbjct: 4   SMRKAIGVVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 63

Query: 65  YVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGF 124
           YV ACV ++S+RL KTRDWIVALKALM+VHR+L +G P F EEI+YATRRG R+LN+S F
Sbjct: 64  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 123

Query: 125 RDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVR--GSISDGNSDESDYGRQRRRQ-- 184
           RDEAHS+SWDHS FVR+YA YLD  +E  + E++ R  G  S  +    D G  R R   
Sbjct: 124 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 183

Query: 185 ----------------------------------------SPWRSMRPERVLDEFDQLLR 244
                                                   +P R M PER+  +   L R
Sbjct: 184 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQR 243

Query: 245 MLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICEALGVLLDHFKEMEYGDCLRA 304
           +LDR L+CRP G+AK+SR+++ A++  VKESF LY +ICE L VLLD F +MEY DC++A
Sbjct: 244 LLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKA 303

Query: 305 FDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITHNLLGTLGDFLKEMGRRPKDF 364
           FD Y +AAK+ID+L+ FY WCKD G+ARSSEYPEVQRIT  LL TL +F+++  +R K  
Sbjct: 304 FDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSP 363

Query: 365 NERRRE-EKLKLPPADDPEQDMNEMKALPPPKNH---DPSLHSVLPQTRLPKEDLVNLRE 424
             +  E      PP ++P  DMNE+KALPPP+NH    P      PQ     +DLVNLRE
Sbjct: 364 ERKEIEAPPAPAPPVEEP-VDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLRE 423

Query: 425 DEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKADWELAL 484
           D+VS D+QGNK ALALF+GPP  N  G WE FSS  D  V+SAW+ PAAE GKADWELAL
Sbjct: 424 DDVSGDDQGNKFALALFAGPPANN--GKWEAFSS--DNNVTSAWQNPAAELGKADWELAL 483

Query: 485 VETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVN-AEQFGGSSSSVALQVQGKTVAA 544
           VETASNL  QKA MGGG DPLLLN MYDQG +R+HV+ +E  GGSSSSVAL + GK V +
Sbjct: 484 VETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPLPGK-VNS 543

Query: 545 QVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREGM 585
            +LALPAPDGTV  V ++DPFAASLT+PPPSYVQ+AEM+KKQ LL QEQ +WQQY +EGM
Sbjct: 544 HILALPAPDGTVQKV-NQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLWQQYQQEGM 603

BLAST of MC06g0590 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 619.4 bits (1596), Expect = 3.0e-177
Identity = 350/657 (53.27%), Postives = 439/657 (66.82%), Query Frame = 0

Query: 5   TIRKAIGAVKDQTSISIAKVAGNTAPELEVLVVKATSHDEDPDDDRYIIEIVNLCSHSNG 64
           +IRKAIGAVKDQTSI IAKVA N AP+LEV +VKATSHD+DP  ++YI EI+NL S S G
Sbjct: 4   SIRKAIGAVKDQTSIGIAKVASNMAPDLEVAIVKATSHDDDPASEKYIREILNLTSLSRG 63

Query: 65  YVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRVLNLSGF 124
           Y+ ACV ++S+RL+KTRDW+VALKALM+VHR+L +G P F EEI+Y+TRRG R+LN+S F
Sbjct: 64  YILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRMLNMSDF 123

Query: 125 RDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSISDG------NSDESDYGR--- 184
           RDEAHS+SWDHS FVR+YA YLD  +E  + E+K   S++ G      ++++  YGR   
Sbjct: 124 RDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRYGRGRD 183

Query: 185 -------------------------------------------------------QRRRQ 244
                                                                  +++  
Sbjct: 184 DFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRDEKKVV 243

Query: 245 SPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAVKESFGLYIEICE 304
           +P R M PER+  +   L R+LDR L+ RP G+AK+SR+++ A++  V+ESF LY +ICE
Sbjct: 244 TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLYADICE 303

Query: 305 ALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIARSSEYPEVQRITH 364
            L VLLD F +MEY DC++AFD Y +AAK+ID+L+ FY WCK+ G+ARSSEYPEVQRIT 
Sbjct: 304 VLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEVQRITS 363

Query: 365 NLLGTLGDFLKEMGRRPKDFNERRREEKLKLPPADDPEQDMNEMKALPPPKNH-DPSLHS 424
            LL TL +F+++  +R K    +  E    +   ++PE DMNE+KALPPP+N+  P    
Sbjct: 364 KLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPE 423

Query: 425 VLPQTRLPK--EDLVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVS 484
             PQ   P+  EDLVNLREDEV+AD+QGNK ALALF+GPP  N  G WE FSSN    V+
Sbjct: 424 PEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAGPPGNN--GKWEAFSSN---GVT 483

Query: 485 SAWKTPAAEAGKADWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQF 544
           SAW+ PAAE GKADWELALVET SNL KQ A +GGGFD LLLN MYDQG +R+HV+  Q 
Sbjct: 484 SAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQL 543

Query: 545 -GGSSSSVALQVQGKTVAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKK 588
            GGS+SSVAL + GKT   QVLALPAPDGTV  V ++DPFAASLT+PPPSYVQ+AEMEKK
Sbjct: 544 TGGSASSVALPLPGKT-NNQVLALPAPDGTVEKV-NQDPFAASLTIPPPSYVQMAEMEKK 603

BLAST of MC06g0590 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 9.1e-118
Identity = 243/603 (40.30%), Postives = 370/603 (61.36%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTA--PELEVLVVKATSHDEDPDDDRYIIEIVNL 60
           M  S  ++AIGAVKDQTS+ +AKV G +A   EL+V +VKAT H+E P +++YI EI++L
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  CSHSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRV 120
            S+S  Y++ACV T+S+RLNKT+ W VALK L+++ R+L +G  ++ +EI +ATRRG R+
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLVLEKKVRGSI--SDGNSDESDYGRQ 180
           LN+S FRD + SNSWD+S FVR+YALYLD  ++  +  +  +  +    G +DE +  + 
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQA 180

Query: 181 RR--------RQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAAVHLAV 240
                     R  P   M+ E++      L ++LDR LACRP G A+++R+VI A++  V
Sbjct: 181 AADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIV 240

Query: 241 KESFGLYIEICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKDMGIAR 300
           KESF +Y ++ E +G+L++ F E++  D ++ +D++   +K+ ++L +FY+WCK+MGIAR
Sbjct: 241 KESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIAR 300

Query: 301 SSEYPEVQRITHNLLGTLGDFLK-----EMGRRPKDFNERRRE--EKLKLPPADDPEQDM 360
           SSEYPE+++IT   L  + +F++     E  ++ K       E  ++ +    ++ ++DM
Sbjct: 301 SSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDM 360

Query: 361 NEMKAL--PPPKNHDPSLHSVLPQTRLPKEDLVNLREDEV------------SADEQGNK 420
           N +KAL  PPPK  D     V P+    +E ++  +++E+             A + G+ 
Sbjct: 361 NAIKALPEPPPKEED----DVKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDS 420

Query: 421 LALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKADWELALVETASNLSKQK 480
           LALALF GP           ++S    E    W+  A +   ADWE ALV+TA+NLS QK
Sbjct: 421 LALALFDGP-----------YASGSGSESGPGWE--AFKDDSADWETALVQTATNLSGQK 480

Query: 481 ADMGGGFDPLLLNAMYDQGTIRRHV---NAEQFGGSSSSVALQVQGKTVAAQVLALPAPD 540
           +++GGGFD LLLN MY  G +   V    A    GS+SS+A    G+  AA +LALPAP 
Sbjct: 481 SELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRP-AATMLALPAPS 540

Query: 541 ------GTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREGMHGQ 562
                 G +      DPFAASL V PP+YVQ+ +MEKKQ++L++EQ++W QY R+G  G 
Sbjct: 541 TANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQGH 585

BLAST of MC06g0590 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 419.5 bits (1077), Expect = 4.5e-117
Identity = 248/609 (40.72%), Postives = 363/609 (59.61%), Query Frame = 0

Query: 1   MAQSTIRKAIGAVKDQTSISIAKVAGNTA--PELEVLVVKATSHDEDPDDDRYIIEIVNL 60
           M  S +++AIGAVKDQTS+ +AKV G ++   ELE+ VVKAT HD+ P +D+YI EI+ L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  CSHSNGYVSACVVTISKRLNKTRDWIVALKALMVVHRVLIDGHPSFGEEIVYATRRGMRV 120
            S+S  YVSACV T+S+RLNKT++W VALK L+++ R+L DG  ++ +EI +ATRRG R+
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNLSGFRDEAHSNSWDHSGFVRSYALYLDGMIEHLV-----LEKKVRGSISDGNSDESDY 180
           LN+S FRD + S+SWD+S FVR+YALYLD  +++ +      +K   G   DG+S E D 
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDD 180

Query: 181 GRQRR----------RQSPWRSMRPERVLDEFDQLLRMLDRVLACRPMGMAKSSRLVIAA 240
            R             +  P   M+ E++ +    L ++LDR LACRP G AK++R+VI A
Sbjct: 181 HRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVA 240

Query: 241 VHLAVKESFGLYIEICEALGVLLDHFKEMEYGDCLRAFDVYVNAAKKIDQLLEFYAWCKD 300
           ++  VKESF LY  I E +GVL++ F E++  D ++ ++++   +K+ D+L  FY WCK+
Sbjct: 241 MYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKN 300

Query: 301 MGIARSSEYPEVQRITHNLLGTLGDFLKEMG-------RRPKDFNERRREEKLKLPPADD 360
           M +ARSSEYPE+++IT   L  + +F+++         +     + +  EE+ K     +
Sbjct: 301 MAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQE 360

Query: 361 PEQDMNEMKALPPPKNHDPSLHSVLPQTRLPKEDLVNLREDE-----------VSADEQG 420
            ++D+N +KALP P+  +        +T+   E++V+ ++ E           V+A   G
Sbjct: 361 NQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVG 420

Query: 421 NKLALALFSGPPITNPDG--SWETFSSNQDPEVSSAWKTPAAEAGKADWELALVETASNL 480
           + LALALF G   T       WE F+ N                  ADWE  LV +A+ L
Sbjct: 421 DSLALALFDGVVGTESASGPGWEAFNDN-----------------SADWETDLVRSATRL 480

Query: 481 SKQKADMGGGFDPLLLNAMYDQGTIRRHV---NAEQFGGSSSSVALQVQGKTVAAQVLAL 540
           S QK+++GGGFD LLL+ MY  G +   V    A    GS+SSVA    G + AA +LAL
Sbjct: 481 SGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAG-SPAASMLAL 540

Query: 541 PAPDGTVTPVGHR------DPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQYGREG 564
           PAP  T    G+R      DPFAASL V PP+YVQ+ +MEKKQ+LL++EQ++W QY R G
Sbjct: 541 PAPPPTAN--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSG 589