MC06g0566 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0566
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionH(+)-exporting diphosphatase
LocationMC06: 4581679 .. 4585899 (-)
RNA-Seq ExpressionMC06g0566
SyntenyMC06g0566
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGTCACCATTCTTCCAGATCTCGGGGCTGAGATTCTGATTCCGGTTTGTTCCGTCATCGGAATCGTCTTCTCCTTGCTGCAGTGGTACTACGTTTCGCAAGTCAAGCTCTCCCCTGCCCGGGATTCCGCCCATAACAACTCCGCCGCTGCCGCCGCTAAGAATGGCTACTCCGACTACCTTATTGAGGAAGAGGAGGGCGTTAATGACCACAACGTCGTCATCAAGTGTGCCGAAATTCAGAACGCTATCTCCGAGGGTAACCTAATCGCCTCCCTCCTCTTCTCTTCTCTTTCGCTTTCGGATTATCGGGTTTTCTACTCAATTATTGCTAGATCTGGTGCTGGATTCTCAGATTTGTGATTCTTAGATTTGTTTTTTTCTCTTTCATGGAGTAATTTTTTGTTTTGTTACATGGAAGCATCGACTTTGGGTTCTTCTATGGTAACTTCCTGATTTTGTTGTTTTTACCCTGGCATCGTGTGTAGTAGATATGTGGATGTTACATTTGGTCTTGTCGTTCCATGCATTTACAGGCCGAGCTTGTTATTTTTATGCTATTACGTTCGGGTTATTACATCAAATAGCATGAGAAAGTTGGTCTCTGGTCATACCGCATTTTCATAAAATACGTTACGCAATAATTAATTACCAAATACGTATTTGATGTGATTGGTTTATCGTGTCTGACTATTTGTCGTTTATTAGTTTAGCAAATTTTAGGGGGTAGAGAGGATTGTGATCTTTTGAGTTCTCCCCCTGCATCGTGTAGTCTGGCATCTTTCAAAGAAACCATGAATTCGTGAATTTATGAAGAGATCTTGAAAGTGATGATTGAATTAATTTCAGTTTATTAAATAACTGAGAATGACGCATTAACAGATTTCTTGGAACTTATTCCATTAAATCAGCCTCGTTTTTATCCATTTGGGTTTGTAGTATATATTTTGGTCATAGGATAGAGTTGCATCATCGCATCTAGAGCTAATCTAATCGGCATTTCTTCCGATTCCTCTTTCTTTGCATCAGATTCTACGTTAGAGTGTTGTTTGTTCTTTGTGATATTTATGTACTTGTTTTTTTTTTCATATATATCTGTGCCAGAACAATATGCATACAAATATTCGGTGTTGAAAATTTTTTTGGGACGTTTATAAGTTTCTAAGTTGGTGTCTTTTGGAAACATTTTTTGCCCTTTGACATTTATGAATTCCTTTTATCAAAGGCGTTGTCTTCCAAAAACTGAAACTCTTATGTGAATATATATATATATATATATATTTTATTGCAGTGAAAAAAGGTAAAAAGTATTATTTTCTCCACTCTGTAAAATATGTAGGAAAATGAATGGTGTAAGATTTATCAGAATATTTGTTCTTTAACATCATATAGTTGTTGGCTCTGATCTTCAATAGTTGATTGGGCTGATTGTTGTCTTTTCACATCCATTTATTCATAAAATGAAGCCTTGTGTAATAATTGCTTTTCTGATGGAGCTGCTTCTAACTATTTTAACTCTTTGATGTATTAGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATATTTATGGTGGCTTTTGCGGTCTTGATTTTCCTATTCCTTGGCTCCGTGGAGAGTTTTAGTACCAAGCCTCAGCCTTGCTCATATGACAAATCAAAGACTTGTAAACCAGCTCTGGCGACAGCTATATTCAGCACTATATCATTTTTACTTGGTGCTTTCACATCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCGAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATCACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTCATCGCCATTAACCTCTTTAAATTGTACTATGGTGATGATTGGGGTGGTCTCTTTGAGTCTATCACTGGTTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAGGCCGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGACCCAAGAAACCCAGCTGTAATGATCCTATATTTTCTTAGTTTATTTTGTTCTGATACTTTTGTCCCTTAATCAAATCACTTAACTGTTTTGATAGGTGATTGCTGATAATGTCGGTGATAATGTTGGAGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCCCTTGTCGTCGCTTCTATATCTTCTTTTGGCAACAACCACGAGTTGACTCCAATGCTCTATCCTCTCATCGTCAGTTCTGTGGGTATCCTTGTTTGCCTGCTCACGACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAGCTCATAATTTCCACTGTTCTAATGACCTTTGGGGTTGCAATTGTAACTTGGATTTCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGATGTGACGAACTGGTTAGTGCTTCTTGAATAACTGTCATAATTTATTTTCATTATCAGTAGCGGTTTGATTCACCAGCTAGTATTTGGCATTCAGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATTATTGGATTTGTGACAGAGTATTATACGAGCAATGCATACAGGTAACTGATTTACTGGCACTTACCTAGTTAATTCTAAATGATTCAATCTATGAAATAAAAATTGAGATCTGATCACATCTTTGTATGGTTTTTCAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCCTTGGGATATAAATCTGTCATTATCCCTATTTTTGCAATTGCAGTTAGCATTTTTGTGAGTTTCACCTTTGCTGCAATGTACGGTATTGCTGTAGCTGCCCTTGGAATGTTAAGTACAATTGCTACTGGTTTGGCCATTGATGCATACGGTCCAATCAGTGACAATGCTGGAGGTATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCAGGAAACACCACCGCCGCCATTGGAAAGGTATATATTTTTTTCAATTCTTTTATGTCTCAATCCTACTGCCTGGACCATTTCCTTATTGGCTTTGGTCTTGGTCTCCGTAGAGTGTCCAGAAGACCCTGCTCCGCCTCCAAGCTTCCAAACAACTCTTTGCTCATCTCTCTGTCAAAAAATTGATTCTGTTTTTGCTTTTTCAGGGTTTCGCTATTGGTTCAGCTGCTCTTGTGTCGCTTGCTCTCTTTGGTGCCTTCGTCAGCCGTGCTGGTGTCAATGCCGTTGACGTCTTGACTCCCAAAGTTTTCATTGGCTTGATCGTGGGCGCTATGCTCCCTTATTGGTTTTCTGCCATGACAATGAAGAGTGTCGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCACTAATTGTCGGTATCCTATTCGGTGTCGAAACTCTTTCTGGCGTCCTTGCTGGCTCTCTTGTTTCTGGTGTGCAGGTAAGAGATGGGCTGATGCCTGGAACTACTAGTTAGAAGTTGAATTTTTTACAAGAACCTTATAATAATAATACCCCCCATCTAACTGAATTGTTCATGTTGGGTAAACCGTAGATTGCCATCTCTGCATCCAACACCGGAGGTGCGTGGGATAACGCCAAGAAGTACATCGAGGTATGTTGAAATCTAATAATCATCTTTACAATGTTGTAAAGATTTTTCGGTATCAGATGTAATAATAGCAAGTTGGTATTGATCATTGTGGACAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCTCACAAAGCAGCTGTTATCGGGGACACCATCGGAGACCCGCTCAAGGATACATCCGGACCATCTCTCAACATTCTGATCAAGTTGATGGCTGTGGAGTCCCTCGTGTTCGCTCCTTTCTTCGCCAGCCACGGTGGGCTTCTTTTCAAGATC

mRNA sequence

ATGGGCGTCACCATTCTTCCAGATCTCGGGGCTGAGATTCTGATTCCGGTTTGTTCCGTCATCGGAATCGTCTTCTCCTTGCTGCAGTGGTACTACGTTTCGCAAGTCAAGCTCTCCCCTGCCCGGGATTCCGCCCATAACAACTCCGCCGCTGCCGCCGCTAAGAATGGCTACTCCGACTACCTTATTGAGGAAGAGGAGGGCGTTAATGACCACAACGTCGTCATCAAGTGTGCCGAAATTCAGAACGCTATCTCCGAGGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATATTTATGGTGGCTTTTGCGGTCTTGATTTTCCTATTCCTTGGCTCCGTGGAGAGTTTTAGTACCAAGCCTCAGCCTTGCTCATATGACAAATCAAAGACTTGTAAACCAGCTCTGGCGACAGCTATATTCAGCACTATATCATTTTTACTTGGTGCTTTCACATCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCGAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATCACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTCATCGCCATTAACCTCTTTAAATTGTACTATGGTGATGATTGGGGTGGTCTCTTTGAGTCTATCACTGGTTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAGGCCGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGACCCAAGAAACCCAGCTGTGATTGCTGATAATGTCGGTGATAATGTTGGAGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCCCTTGTCGTCGCTTCTATATCTTCTTTTGGCAACAACCACGAGTTGACTCCAATGCTCTATCCTCTCATCGTCAGTTCTGTGGGTATCCTTGTTTGCCTGCTCACGACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAGCTCATAATTTCCACTGTTCTAATGACCTTTGGGGTTGCAATTGTAACTTGGATTTCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGATGTGACGAACTGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATTATTGGATTTGTGACAGAGTATTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCCTTGGGATATAAATCTGTCATTATCCCTATTTTTGCAATTGCAGTTAGCATTTTTGTGAGTTTCACCTTTGCTGCAATGTACGGTATTGCTGTAGCTGCCCTTGGAATGTTAAGTACAATTGCTACTGGTTTGGCCATTGATGCATACGGTCCAATCAGTGACAATGCTGGAGGTATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCAGGAAACACCACCGCCGCCATTGGAAAGGGTTTCGCTATTGGTTCAGCTGCTCTTGTGTCGCTTGCTCTCTTTGGTGCCTTCGTCAGCCGTGCTGGTGTCAATGCCGTTGACGTCTTGACTCCCAAAGTTTTCATTGGCTTGATCGTGGGCGCTATGCTCCCTTATTGGTTTTCTGCCATGACAATGAAGAGTGTCGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCACTAATTGTCGGTATCCTATTCGGTGTCGAAACTCTTTCTGGCGTCCTTGCTGGCTCTCTTGTTTCTGGTGTGCAGATTGCCATCTCTGCATCCAACACCGGAGGTGCGTGGGATAACGCCAAGAAGTACATCGAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCTCACAAAGCAGCTGTTATCGGGGACACCATCGGAGACCCGCTCAAGGATACATCCGGACCATCTCTCAACATTCTGATCAAGTTGATGGCTGTGGAGTCCCTCGTGTTCGCTCCTTTCTTCGCCAGCCACGGTGGGCTTCTTTTCAAGATC

Coding sequence (CDS)

ATGGGCGTCACCATTCTTCCAGATCTCGGGGCTGAGATTCTGATTCCGGTTTGTTCCGTCATCGGAATCGTCTTCTCCTTGCTGCAGTGGTACTACGTTTCGCAAGTCAAGCTCTCCCCTGCCCGGGATTCCGCCCATAACAACTCCGCCGCTGCCGCCGCTAAGAATGGCTACTCCGACTACCTTATTGAGGAAGAGGAGGGCGTTAATGACCACAACGTCGTCATCAAGTGTGCCGAAATTCAGAACGCTATCTCCGAGGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATATTTATGGTGGCTTTTGCGGTCTTGATTTTCCTATTCCTTGGCTCCGTGGAGAGTTTTAGTACCAAGCCTCAGCCTTGCTCATATGACAAATCAAAGACTTGTAAACCAGCTCTGGCGACAGCTATATTCAGCACTATATCATTTTTACTTGGTGCTTTCACATCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCGAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATCACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTCATCGCCATTAACCTCTTTAAATTGTACTATGGTGATGATTGGGGTGGTCTCTTTGAGTCTATCACTGGTTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAGGCCGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGACCCAAGAAACCCAGCTGTGATTGCTGATAATGTCGGTGATAATGTTGGAGATATAGCCGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCCCTTGTCGTCGCTTCTATATCTTCTTTTGGCAACAACCACGAGTTGACTCCAATGCTCTATCCTCTCATCGTCAGTTCTGTGGGTATCCTTGTTTGCCTGCTCACGACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAGCTCATAATTTCCACTGTTCTAATGACCTTTGGGGTTGCAATTGTAACTTGGATTTCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGATGTGACGAACTGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATTATTGGATTTGTGACAGAGTATTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCCTTGGGATATAAATCTGTCATTATCCCTATTTTTGCAATTGCAGTTAGCATTTTTGTGAGTTTCACCTTTGCTGCAATGTACGGTATTGCTGTAGCTGCCCTTGGAATGTTAAGTACAATTGCTACTGGTTTGGCCATTGATGCATACGGTCCAATCAGTGACAATGCTGGAGGTATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTTGATGCTGCAGGAAACACCACCGCCGCCATTGGAAAGGGTTTCGCTATTGGTTCAGCTGCTCTTGTGTCGCTTGCTCTCTTTGGTGCCTTCGTCAGCCGTGCTGGTGTCAATGCCGTTGACGTCTTGACTCCCAAAGTTTTCATTGGCTTGATCGTGGGCGCTATGCTCCCTTATTGGTTTTCTGCCATGACAATGAAGAGTGTCGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACGCCACTAATTGTCGGTATCCTATTCGGTGTCGAAACTCTTTCTGGCGTCCTTGCTGGCTCTCTTGTTTCTGGTGTGCAGATTGCCATCTCTGCATCCAACACCGGAGGTGCGTGGGATAACGCCAAGAAGTACATCGAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCTCACAAAGCAGCTGTTATCGGGGACACCATCGGAGACCCGCTCAAGGATACATCCGGACCATCTCTCAACATTCTGATCAAGTTGATGGCTGTGGAGTCCCTCGTGTTCGCTCCTTTCTTCGCCAGCCACGGTGGGCTTCTTTTCAAGATC

Protein sequence

MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Homology
BLAST of MC06g0566 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 710/769 (92.33%), Postives = 741/769 (96.36%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           MG  ILPDLG EILIPVC+VIGI F+L QW  VS+VKLS  RD++ N    AAAKNGY+D
Sbjct: 1   MGAAILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN----AAAKNGYND 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEG+NDHNVV+KCAEIQNAISEGATSFLFTEYKYVGIFMVAFA+LIFLFLGSVE 
Sbjct: 61  YLIEEEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEG 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FST PQ CSYDK+KTCKPALATAIFST+SFLLG  TS+VSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVL+IAINLFK+YYGDDWGGLFE+ITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFG NHELT MLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQL+ISTVLMT GVA+V+++++P++FTIFNFG QKDV +W+LFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRA +  VDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPL+V
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHAR+LGPKGSD H
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 770
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKI 765

BLAST of MC06g0566 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 673/766 (87.86%), Postives = 718/766 (93.73%), Query Frame = 0

Query: 3   VTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYL 62
           + IL +LG EILIPVC VIGIVF++ QW+ VS+VK++P        SAAA AKNGY DYL
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGA-----ASAAAGAKNGYGDYL 60

Query: 63  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 122
           IEEEEG+NDHNVV+KCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+E FS
Sbjct: 61  IEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFS 120

Query: 123 TKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 182
           TK QPC+Y K  TCKPAL TA+FST SFLLGA TS+VSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKGQPCTYSKG-TCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKG 180

Query: 183 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 242
           VGKAFITAFRSGAVMGFLL+++GL+VL+I IN+FK+YYGDDW GLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMA 240

Query: 243 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 302
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 303 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 362
           YAESSCAALVVASISSFG NH+ T M YPL+VSSVGI+VCLLTTLFATDFFEIKA  EIE
Sbjct: 301 YAESSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIE 360

Query: 363 PALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLII 422
           PALKKQLIIST LMT GVA+++W+++P+ FTIFNFG QK+V+NW LF CVAVGLWAGLII
Sbjct: 361 PALKKQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLII 420

Query: 423 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 482
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI+VSF+ AA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAA 480

Query: 483 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 542
           MYGIA+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 543 IGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 602
           IGKGFAIGSAALVSLALFGAFVSRAGV  VDVL+PKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 603 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 662
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGT 660

Query: 663 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 722
           LFGVETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKA
Sbjct: 661 LFGVETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKA 720

Query: 723 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA++GGLLFK
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFK 760

BLAST of MC06g0566 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 664/764 (86.91%), Postives = 717/764 (93.85%), Query Frame = 0

Query: 5   ILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYLIE 64
           +LP+L  EIL+P+C+VIGI FSL QWY VS+VKL+ +   A ++  A   KNGY DYLIE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLT-SDLGASSSGGANNGKNGYGDYLIE 65

Query: 65  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 124
           EEEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST 
Sbjct: 66  EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTD 125

Query: 125 PQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 184
            +PC+YD ++TCKPALATA FSTI+F+LGA TSV+SGFLGMKIATYANARTTLEARKGVG
Sbjct: 126 NKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVG 185

Query: 185 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 244
           KAFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALF
Sbjct: 186 KAFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALF 245

Query: 245 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 304
           GRVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 246 GRVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 305

Query: 305 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPA 364
           E+SCAALVVASISSFG NH+ T M YPL++SS+GILVCL+TTLFATDFFEIK VKEIEPA
Sbjct: 306 EASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPA 365

Query: 365 LKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGF 424
           LK QLIISTV+MT G+AIV+W+ +P++FTIFNFGTQK V NW+LFLCV VGLWAGLIIGF
Sbjct: 366 LKNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGF 425

Query: 425 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 484
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMY
Sbjct: 426 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMY 485

Query: 485 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 544
           G+AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 486 GVAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 545

Query: 545 KGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 604
           KGFAIGSAALVSLALFGAFVSRAG++ VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSA
Sbjct: 546 KGFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSA 605

Query: 605 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 664
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  F
Sbjct: 606 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFF 665

Query: 665 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 724
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAV
Sbjct: 666 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAV 725

Query: 725 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 769
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK
Sbjct: 726 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of MC06g0566 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.2e-199
Identity = 397/696 (57.04%), Postives = 503/696 (72.27%), Query Frame = 0

Query: 77  KCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCSYDKSKTC 136
           K  EI +AISEGA +FL  EYK + +F+   AVLI L L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 137 KPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 196
                  I++ I+F+ GA  S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 197 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 256
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 257 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 316
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 317 ASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPALKKQLIIST 376
            + +S   +  +  +LYPL++S+ GI   +LT+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 377 VLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 436
           +L+   +  VT   +  +F I      K +T W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 437 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 496
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 497 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 556
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 557 LVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 616
           L SLALF AF++R    +++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 617 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 676
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 677 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 736
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 737 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 770
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKI
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of MC06g0566 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.2e-199
Identity = 397/696 (57.04%), Postives = 503/696 (72.27%), Query Frame = 0

Query: 77  KCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCSYDKSKTC 136
           K  EI +AISEGA +FL  EYK + +F+   AVLI L L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 137 KPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 196
                  I++ I+F+ GA  S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 197 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 256
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 257 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 316
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 317 ASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPALKKQLIIST 376
            + +S   +  +  +LYPL++S+ GI   +LT+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 377 VLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 436
           +L+   +  VT   +  +F I      K +T W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 437 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 496
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 497 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 556
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 557 LVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 616
           L SLALF AF++R    +++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 617 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 676
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 677 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 736
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 737 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 770
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKI
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of MC06g0566 vs. NCBI nr
Match: XP_022135071.1 (pyrophosphate-energized vacuolar membrane proton pump [Momordica charantia])

HSP 1 Score: 1447 bits (3745), Expect = 0.0
Identity = 769/769 (100.00%), Postives = 769/769 (100.00%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD
Sbjct: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769

BLAST of MC06g0566 vs. NCBI nr
Match: XP_022929683.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] >KAG6587868.1 hypothetical protein SDJN03_16433, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021755.1 hypothetical protein SDJN02_15482 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1403 bits (3632), Expect = 0.0
Identity = 742/769 (96.49%), Postives = 758/769 (98.57%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+V+GIVFSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. NCBI nr
Match: XP_023002108.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima])

HSP 1 Score: 1403 bits (3632), Expect = 0.0
Identity = 742/769 (96.49%), Postives = 758/769 (98.57%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+VIGI+FSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. NCBI nr
Match: XP_023529940.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1401 bits (3627), Expect = 0.0
Identity = 741/769 (96.36%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+V+GIVFSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQCAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLI+SS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIISSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. NCBI nr
Match: BAA33149.1 (proton-translocating inorganic pyrophosphatase [Cucurbita moschata])

HSP 1 Score: 1400 bits (3624), Expect = 0.0
Identity = 741/769 (96.36%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+VIGIVFSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASIS FGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. ExPASy TrEMBL
Match: A0A6J1BZK8 (H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111007149 PE=3 SV=1)

HSP 1 Score: 1447 bits (3745), Expect = 0.0
Identity = 769/769 (100.00%), Postives = 769/769 (100.00%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD
Sbjct: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769

BLAST of MC06g0566 vs. ExPASy TrEMBL
Match: A0A6J1KSL0 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV=1)

HSP 1 Score: 1403 bits (3632), Expect = 0.0
Identity = 742/769 (96.49%), Postives = 758/769 (98.57%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+VIGI+FSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. ExPASy TrEMBL
Match: A0A6J1ESX2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 SV=1)

HSP 1 Score: 1403 bits (3632), Expect = 0.0
Identity = 742/769 (96.49%), Postives = 758/769 (98.57%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+V+GIVFSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. ExPASy TrEMBL
Match: O82680 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 1400 bits (3624), Expect = 0.0
Identity = 741/769 (96.36%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60
           M VTILPDLG EI IPVC+VIGIVFSL+QWYYVSQVKLSP RD+AHNNSA +  KNGYSD
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS--KNGYSD 60

Query: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVES 120

Query: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK++TCKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASIS FGNNHELTPMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKE 360

Query: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420
           IEPALKKQLIISTVLMTFG+AIVTW+SVPS+FTIFNFGTQK VTNWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of MC06g0566 vs. ExPASy TrEMBL
Match: A0A6J1H8E2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 SV=1)

HSP 1 Score: 1391 bits (3600), Expect = 0.0
Identity = 735/767 (95.83%), Postives = 754/767 (98.31%), Query Frame = 0

Query: 3   VTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYL 62
           VTILPDLG +I IPVC+V+GIVFSL+QWYYVSQVKLSP+RDS HNNSAAA  KNGYSDYL
Sbjct: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAA--KNGYSDYL 63

Query: 63  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 122
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVE FS
Sbjct: 64  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFS 123

Query: 123 TKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 182
           T+PQ C+YDK+K CKPALATAIFSTISFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 124 TEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 183

Query: 183 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 242
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMA
Sbjct: 184 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMA 243

Query: 243 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 302
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 244 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 303

Query: 303 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 362
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 304 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIE 363

Query: 363 PALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLII 422
           PALKKQLIISTVLMTFG+A+VTW++VPSTFTIFNFGTQK V NWKLFLCVAVGLWAGLII
Sbjct: 364 PALKKQLIISTVLMTFGIAVVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLII 423

Query: 423 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 482
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 424 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 483

Query: 483 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 542
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 484 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 543

Query: 543 IGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 602
           IGKGFAIGSAALVSLALFGAFVSRAGV +VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 544 IGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 603

Query: 603 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 662
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 604 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 663

Query: 663 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 722
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 664 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 723

Query: 723 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLFK+
Sbjct: 724 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of MC06g0566 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 664/764 (86.91%), Postives = 717/764 (93.85%), Query Frame = 0

Query: 5   ILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYLIE 64
           +LP+L  EIL+P+C+VIGI FSL QWY VS+VKL+ +   A ++  A   KNGY DYLIE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLT-SDLGASSSGGANNGKNGYGDYLIE 65

Query: 65  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 124
           EEEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST 
Sbjct: 66  EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTD 125

Query: 125 PQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 184
            +PC+YD ++TCKPALATA FSTI+F+LGA TSV+SGFLGMKIATYANARTTLEARKGVG
Sbjct: 126 NKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVG 185

Query: 185 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 244
           KAFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALF
Sbjct: 186 KAFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALF 245

Query: 245 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 304
           GRVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 246 GRVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 305

Query: 305 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPA 364
           E+SCAALVVASISSFG NH+ T M YPL++SS+GILVCL+TTLFATDFFEIK VKEIEPA
Sbjct: 306 EASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPA 365

Query: 365 LKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGF 424
           LK QLIISTV+MT G+AIV+W+ +P++FTIFNFGTQK V NW+LFLCV VGLWAGLIIGF
Sbjct: 366 LKNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGF 425

Query: 425 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 484
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMY
Sbjct: 426 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMY 485

Query: 485 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 544
           G+AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 486 GVAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 545

Query: 545 KGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 604
           KGFAIGSAALVSLALFGAFVSRAG++ VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSA
Sbjct: 546 KGFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSA 605

Query: 605 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 664
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  F
Sbjct: 606 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFF 665

Query: 665 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 724
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAV
Sbjct: 666 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAV 725

Query: 725 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 769
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK
Sbjct: 726 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of MC06g0566 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1031.2 bits (2665), Expect = 4.3e-301
Identity = 538/629 (85.53%), Postives = 586/629 (93.16%), Query Frame = 0

Query: 5   ILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSDYLIE 64
           +LP+L  EIL+P+C+VIGI FSL QWY VS+VKL+ +   A ++  A   KNGY DYLIE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLT-SDLGASSSGGANNGKNGYGDYLIE 65

Query: 65  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 124
           EEEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST 
Sbjct: 66  EEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTD 125

Query: 125 PQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 184
            +PC+YD ++TCKPALATA FSTI+F+LGA TSV+SGFLGMKIATYANARTTLEARKGVG
Sbjct: 126 NKPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVG 185

Query: 185 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 244
           KAFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALF
Sbjct: 186 KAFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALF 245

Query: 245 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 304
           GRVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 246 GRVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 305

Query: 305 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPA 364
           E+SCAALVVASISSFG NH+ T M YPL++SS+GILVCL+TTLFATDFFEIK VKEIEPA
Sbjct: 306 EASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPA 365

Query: 365 LKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGLIIGF 424
           LK QLIISTV+MT G+AIV+W+ +P++FTIFNFGTQK V NW+LFLCV VGLWAGLIIGF
Sbjct: 366 LKNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGF 425

Query: 425 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 484
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMY
Sbjct: 426 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMY 485

Query: 485 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 544
           G+AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 486 GVAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 545

Query: 545 KGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 604
           KGFAIGSAALVSLALFGAFVSRAG++ VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSA
Sbjct: 546 KGFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSA 605

Query: 605 ALKMVEEVRRQFNTIPGLMEGTAKPDYAT 634
           ALKMVEEVRRQFNTIPGLMEGTAKPDYAT
Sbjct: 606 ALKMVEEVRRQFNTIPGLMEGTAKPDYAT 633

BLAST of MC06g0566 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 8.3e-119
Identity = 292/734 (39.78%), Postives = 419/734 (57.08%), Query Frame = 0

Query: 80  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCSYDKSKTC 139
           +I +AI +GA  FL T+Y  +    F++AF +L I+LF       +  PQ  +    +T 
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLFR------NLTPQQEASGLGRT- 209

Query: 140 KPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 199
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 210 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 269

Query: 200 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 259
              ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGG
Sbjct: 270 SALVVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGG 329

Query: 260 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 319
           IYTK ADVGADLVGKVE  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 330 IYTKGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 389

Query: 320 LVVASISSFGNNHE------LTPML---YPLIVSSVGILVCLLTTLFATDFFEIKA-VKE 379
           +++    +     E      L P++   + L++SS+GIL     ++  T    +K+ V++
Sbjct: 390 MILGGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGIL-----SIKGTRNASVKSPVED 449

Query: 380 IEPALKK--QLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNW-KLFLCVAVGLW 439
               L+K   L I   ++TFG A   W+            T++  + W   F+C  VG+ 
Sbjct: 450 PMVVLQKGYSLTIILAVLTFG-ASTRWL----------LYTEQAPSAWLNFFMCGLVGII 509

Query: 440 AGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVS 499
              +  +++ YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  +
Sbjct: 510 TAYVFVWISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISA 569

Query: 500 FTF--------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMS 559
           F                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+   
Sbjct: 570 FWLGNTSGLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQP 629

Query: 560 HRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVNA------VDVLTP 619
             +RE TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P
Sbjct: 630 ESVREITDVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIP 689

Query: 620 KVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKI 679
           +VFIG ++GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV I
Sbjct: 690 EVFIGGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAI 749

Query: 680 STDASIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAI 739
              ++++EMI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A+
Sbjct: 750 VASSALREMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMAL 809

Query: 740 SASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILI 760
             +  GGAWDNAKKYIE GA      LG KGSD HKAAV GDT+GDP KDT+GPS+++LI
Sbjct: 810 FLNTAGGAWDNAKKYIETGA------LGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLI 850

BLAST of MC06g0566 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 421.0 bits (1081), Expect = 2.0e-117
Identity = 292/737 (39.62%), Postives = 420/737 (56.99%), Query Frame = 0

Query: 80  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCSYDKSKTC 139
           EI +AI +GA  F  T+Y  +     ++AF +L I+LF     S + + +     ++   
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYLF----RSLTPQQEAAGLGRA--- 160

Query: 140 KPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 199
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 161 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 220

Query: 200 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 259
              ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGG
Sbjct: 221 SALVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGG 280

Query: 260 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 319
           IYTK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 281 IYTKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 340

Query: 320 LVVASISSFGNNHE------LTPML---YPLIVSSVGILVCLLTTLFATDFFEIKA-VKE 379
           +++    +     E      L P++   + LI+SS+GIL     ++  T    +K+ V++
Sbjct: 341 MILGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL-----SIKGTRDASVKSPVED 400

Query: 380 IEPALKK--QLIISTVLMTFGVAIVTWI----SVPSTFTIFNFGTQKDVTNWKLFLCVAV 439
               L+K   L I   ++TFG A   W+      PS +  FNF            LC  V
Sbjct: 401 PMAVLQKGYSLTIILAVITFG-ASTRWLLYTEQAPSAW--FNFA-----------LCGLV 460

Query: 440 GLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI 499
           G+    I  ++++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I
Sbjct: 461 GIITAYIFVWISKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAI 520

Query: 500 FVSFTF--------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMA 559
             ++                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+
Sbjct: 521 ISAYWLGNTSGLVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMS 580

Query: 560 GMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVNA------VDV 619
                +RE TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+
Sbjct: 581 QQPESVREITDLLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDI 640

Query: 620 LTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATC 679
             P+VF+G ++GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ C
Sbjct: 641 AIPEVFVGGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRC 700

Query: 680 VKISTDASIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQ 739
           V I   A+++EMI PGAL + +P++VG++F            G + ++ +L  + V G+ 
Sbjct: 701 VAIVASAALREMIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGIL 760

Query: 740 IAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLN 760
           +A+  +  GGAWDNAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS++
Sbjct: 761 MALFLNTAGGAWDNAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIH 801

BLAST of MC06g0566 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 421.0 bits (1081), Expect = 2.0e-117
Identity = 292/737 (39.62%), Postives = 420/737 (56.99%), Query Frame = 0

Query: 80  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCSYDKSKTC 139
           EI +AI +GA  F  T+Y  +     ++AF +L I+LF     S + + +     ++   
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYLF----RSLTPQQEAAGLGRA--- 160

Query: 140 KPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 199
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 161 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 220

Query: 200 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 259
              ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGG
Sbjct: 221 SALVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGG 280

Query: 260 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 319
           IYTK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 281 IYTKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 340

Query: 320 LVVASISSFGNNHE------LTPML---YPLIVSSVGILVCLLTTLFATDFFEIKA-VKE 379
           +++    +     E      L P++   + LI+SS+GIL     ++  T    +K+ V++
Sbjct: 341 MILGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL-----SIKGTRDASVKSPVED 400

Query: 380 IEPALKK--QLIISTVLMTFGVAIVTWI----SVPSTFTIFNFGTQKDVTNWKLFLCVAV 439
               L+K   L I   ++TFG A   W+      PS +  FNF            LC  V
Sbjct: 401 PMAVLQKGYSLTIILAVITFG-ASTRWLLYTEQAPSAW--FNFA-----------LCGLV 460

Query: 440 GLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI 499
           G+    I  ++++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I
Sbjct: 461 GIITAYIFVWISKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAI 520

Query: 500 FVSFTF--------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMA 559
             ++                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+
Sbjct: 521 ISAYWLGNTSGLVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMS 580

Query: 560 GMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVNA------VDV 619
                +RE TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+
Sbjct: 581 QQPESVREITDLLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDI 640

Query: 620 LTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATC 679
             P+VF+G ++GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ C
Sbjct: 641 AIPEVFVGGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRC 700

Query: 680 VKISTDASIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQ 739
           V I   A+++EMI PGAL + +P++VG++F            G + ++ +L  + V G+ 
Sbjct: 701 VAIVASAALREMIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGIL 760

Query: 740 IAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLN 760
           +A+  +  GGAWDNAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS++
Sbjct: 761 MALFLNTAGGAWDNAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIH 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P216160.0e+0092.33Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
Q065720.0e+0087.86Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
P314140.0e+0086.91Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
Q72Q291.2e-19957.04Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Q8F6411.2e-19957.04Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
XP_022135071.10.0100.00pyrophosphate-energized vacuolar membrane proton pump [Momordica charantia][more]
XP_022929683.10.096.49pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] ... [more]
XP_023002108.10.096.49pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima][more]
XP_023529940.10.096.36pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp... [more]
BAA33149.10.096.36proton-translocating inorganic pyrophosphatase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BZK80.0100.00H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111007149 PE=3... [more]
A0A6J1KSL00.096.49H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV... [more]
A0A6J1ESX20.096.49H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 ... [more]
O826800.096.36H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1[more]
A0A6J1H8E20.095.83H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0086.91Inorganic H pyrophosphatase family protein [more]
AT1G15690.24.3e-30185.53Inorganic H pyrophosphatase family protein [more]
AT1G16780.18.3e-11939.78Inorganic H pyrophosphatase family protein [more]
AT1G78920.12.0e-11739.62vacuolar H+-pyrophosphatase 2 [more]
AT1G78920.22.0e-11739.62vacuolar H+-pyrophosphatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 11..768
e-value: 1.7E-282
score: 937.2
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 11..765
e-value: 0.0
score: 1228.2
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 47..755
e-value: 9.8E-257
score: 853.8
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMPcoord: 1..769
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 9..760
score: 23.03937
NoneNo IPR availablePANTHERPTHR31998:SF38PYROPHOSPHATE-ENERGIZED VACUOLAR MEMBRANE PROTON PUMPcoord: 1..769

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0566.1MC06g0566.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004427 inorganic diphosphatase activity
molecular_function GO:0009678 pyrophosphate hydrolysis-driven proton transmembrane transporter activity