MC06g0564 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0564
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	MC06: 4577459 .. 4578933 (+)
RNA-Seq Expression	MC06g0564
Synteny	MC06g0564

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGGAACTAACCCTACCGGCAGCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAGAGATATATCCATAAAGAATGCGATCGGGGAGCTCTAGATGCTCCATTAATGGAAGCTCCTGTGATTGATATCGGTCTCCTCTCGTCTCCATCCAATACCGGACCGGAACTGGAGAAACTCCGACATGGACTTCACTCTTGGGGCTGCTTTCAGGTTTGATTTCTGTAGAATTTGCTCTCTTAAACCCTAGATCGGGGGTGCGGGGGGTTAGGAATTTCTTTAAACCCTAATTTCAATTGACTCTGCATATCGATCCCATAAAACGTTCGGAGATCGTAAATAGCCTGAAAATACGCCCGGATTTTGATTGCTTAATGTTTTGAAAACTTGATCGGAAGTTTTCTGGTGTTCTTTCAGGCAATAAATCATGGAATGTCACCTGAATTTCTGGAAGAAGTGCGCCAAGTAGCGAAACTGTTCTTCGCTCTTCCAATGGAAGACAAACTGAAACATTCCCGAGAAGAAGATAAGATGGAAGGATATGGCAACGACATGATTTTCTCCAACCAACAAATTCTCGATTGGACTGATCGTTTATACCTCACTGTATGTCCAGAAGAAAGCCGCCGTTTCAAGTACTGGCCAACAAATCCTGAAAGATTCAGGTACTTTCCCAATCTTCTTAATTCCCTCAACAATTTTTATATGTGTTTTGGTTTATGGGTAGTAAACAAGAGTCTGCAGAAGCTGTTGGTTCTGAAACATGTTTCGGATCGAACAAGTGAATGATGAATATGATGTTGTGATGAAAATTTCAGGGAAGTTCTTCACGAGTACACTGCAAATGTGAAGCTTTTAAGTGAGAAAATCCTCAAGGCCATGGCAAGGTCACTGGATTTAGATGAGAATAGCTTTCTCAACCAGTATGGGAAGCGAGTTCAACTCGACGCACGGTTCAACTTCTACCTGCGATGTCGAAATCCCAATCTCGTTCTTGGTGTCAAGCCACATGCTGATGGATCAGCAATCACCATTTTGTTGCAGGACAAAGAAGTAGAAGGTCTTCAGTTCTTGAAAGGCAATGAGTGGTGGAACGCGCCGATCATCCCTGATGCGCTTCTCGTCAACGTCGGGGATCAAGGAGAGGTAATCTGATCTGAACTTGAAAGAAAACAATGCACATTGTAGCAGTGAGTTGAGTTGGATAATGAAATGAATTTGGTGATGAAATTTTGCAGATAACAAGTAATGGGATATTCAAGAGTAGAGTTCATAGGGTGTTGACAAACTCAGAGAGGGAGAGGATTTCTTTGGCTGTGTTTTACCTTCCAGATCCACAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCACCCAAGGTTGTACAGAACTGTGAAGAATTTTGTCGGCCTTTTTTTCCAGTACTACCAGCAGGGCCAGAGACCAAGGGAGGCTGCAAAAATC

mRNA sequence

ATGGCCGGAACTAACCCTACCGGCAGCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAGAGATATATCCATAAAGAATGCGATCGGGGAGCTCTAGATGCTCCATTAATGGAAGCTCCTGTGATTGATATCGGTCTCCTCTCGTCTCCATCCAATACCGGACCGGAACTGGAGAAACTCCGACATGGACTTCACTCTTGGGGCTGCTTTCAGGCAATAAATCATGGAATGTCACCTGAATTTCTGGAAGAAGTGCGCCAAGTAGCGAAACTGTTCTTCGCTCTTCCAATGGAAGACAAACTGAAACATTCCCGAGAAGAAGATAAGATGGAAGGATATGGCAACGACATGATTTTCTCCAACCAACAAATTCTCGATTGGACTGATCGTTTATACCTCACTGTATGTCCAGAAGAAAGCCGCCGTTTCAAGTACTGGCCAACAAATCCTGAAAGATTCAGGGAAGTTCTTCACGAGTACACTGCAAATGTGAAGCTTTTAAGTGAGAAAATCCTCAAGGCCATGGCAAGGTCACTGGATTTAGATGAGAATAGCTTTCTCAACCAGTATGGGAAGCGAGTTCAACTCGACGCACGGTTCAACTTCTACCTGCGATGTCGAAATCCCAATCTCGTTCTTGGTGTCAAGCCACATGCTGATGGATCAGCAATCACCATTTTGTTGCAGGACAAAGAAGTAGAAGGTCTTCAGTTCTTGAAAGGCAATGAGTGGTGGAACGCGCCGATCATCCCTGATGCGCTTCTCGTCAACGTCGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTAGAGTTCATAGGGTGTTGACAAACTCAGAGAGGGAGAGGATTTCTTTGGCTGTGTTTTACCTTCCAGATCCACAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCACCCAAGGTTGTACAGAACTGTGAAGAATTTTGTCGGCCTTTTTTTCCAGTACTACCAGCAGGGCCAGAGACCAAGGGAGGCTGCAAAAATC

Coding sequence (CDS)

ATGGCCGGAACTAACCCTACCGGCAGCGTCCAAGACGTGGCTTCTAAAGGGGAAGTGCCGGAGAGATATATCCATAAAGAATGCGATCGGGGAGCTCTAGATGCTCCATTAATGGAAGCTCCTGTGATTGATATCGGTCTCCTCTCGTCTCCATCCAATACCGGACCGGAACTGGAGAAACTCCGACATGGACTTCACTCTTGGGGCTGCTTTCAGGCAATAAATCATGGAATGTCACCTGAATTTCTGGAAGAAGTGCGCCAAGTAGCGAAACTGTTCTTCGCTCTTCCAATGGAAGACAAACTGAAACATTCCCGAGAAGAAGATAAGATGGAAGGATATGGCAACGACATGATTTTCTCCAACCAACAAATTCTCGATTGGACTGATCGTTTATACCTCACTGTATGTCCAGAAGAAAGCCGCCGTTTCAAGTACTGGCCAACAAATCCTGAAAGATTCAGGGAAGTTCTTCACGAGTACACTGCAAATGTGAAGCTTTTAAGTGAGAAAATCCTCAAGGCCATGGCAAGGTCACTGGATTTAGATGAGAATAGCTTTCTCAACCAGTATGGGAAGCGAGTTCAACTCGACGCACGGTTCAACTTCTACCTGCGATGTCGAAATCCCAATCTCGTTCTTGGTGTCAAGCCACATGCTGATGGATCAGCAATCACCATTTTGTTGCAGGACAAAGAAGTAGAAGGTCTTCAGTTCTTGAAAGGCAATGAGTGGTGGAACGCGCCGATCATCCCTGATGCGCTTCTCGTCAACGTCGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTAGAGTTCATAGGGTGTTGACAAACTCAGAGAGGGAGAGGATTTCTTTGGCTGTGTTTTACCTTCCAGATCCACAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCACCCAAGGTTGTACAGAACTGTGAAGAATTTTGTCGGCCTTTTTTTCCAGTACTACCAGCAGGGCCAGAGACCAAGGGAGGCTGCAAAAATC

Protein sequence

MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEKLRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFSNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLDLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEIEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI

Homology

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BLAST of MC06g0564 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 119/321 (37.07%), Postives = 176/321 (54.83%), Query Frame = 0


Query: 6   PTGSVQDVASKG--EVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTG-PE-LEKL 65
           P  SVQ ++  G   VP RY+     R   +    +A  I+I +L      G PE L  +
Sbjct: 8   PIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRLV 67

Query: 66  RHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFS 125
           R     WG FQ +NHG++   +E VR   + FF LP+E+K K++   D  EGYG+ +   
Sbjct: 68  RSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVV 127

Query: 126 NQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLD 185
               LDW+D  +L   P   R    WP+ P + RE++ +Y   V+ L E++ + ++ SL 
Sbjct: 128 KDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLG 187

Query: 186 LDENSFLNQY--GKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQF 245
           L  N  +     G +V    R NFY +C  P L LG+  H+D   ITILL D++V GLQ 
Sbjct: 188 LKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV 247

Query: 246 LKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQK 305
            +G+ W     +P+AL+VN+GDQ +I SNGI+KS  H+V+ NS  ER+SLA FY P    
Sbjct: 248 RRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDI 307

Query: 306 EIEPLEKLINETHPRLYRTVK 321
            + P+E+L+    P LY+ ++
Sbjct: 308 PVGPIEELVTANRPALYKPIR 327

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BLAST of MC06g0564 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.9e-54
Identity = 111/330 (33.64%), Postives = 182/330 (55.15%), Query Frame = 0


Query: 19  VPERYIHKECDRGALDAPL---MEAPVIDIGLLSSPSNTGPELEKLRHGLHSWGCFQAIN 78
           VP RY+  + D+  +D      +E P+ID+  L S +    E+EKL      WG FQ +N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 79  HGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFSNQQILDWTDRLYLT 138
           HG+   FL++V+   + FF LPME+K K  +  D++EG+G   + S  Q LDW D  + T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 139 VCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLDLDENSFLNQYGKRV 198
           V P E R+   +P  P  FR+ L  Y++ V+ +++ ++  MAR+L++        +    
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 199 QLDA-RFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLKGNEWWNAPIIPDA 258
            + + R N+Y  C  P+ V+G+ PH+D   +T+L+Q  +VEGLQ  K  +W     +P+A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 259 LLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEIEPLEKLINETHPR 318
            +VN+GD  EI +NG ++S  HR + NSE+ER+S+A F+     KE+ P + L+      
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 319 LYR--TVKNFVGLFFQYYQQGQRPREAAKI 343
            ++  T+K +    F     G+   +A +I
Sbjct: 329 RFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of MC06g0564 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.0e-53
Identity = 113/319 (35.42%), Postives = 171/319 (53.61%), Query Frame = 0


Query: 6   PTGSVQDVASK--GEVPERYIHKECDRGAL--DAP-LMEAPVIDIGLLSSPSNTGPE--L 65
           P   VQ +A      +P+RYI     R     DAP     P+ID+  L S      +  +
Sbjct: 23  PIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIM 82

Query: 66  EKLRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDM 125
            ++      WG FQ +NHG+ PE ++  R+  + FF +P+  K  +S      EGYG+ +
Sbjct: 83  ARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRL 142

Query: 126 IFSNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAR 185
                  LDW+D  +L + P   + F  WP+ P   REV+ EY   +  LS +I++ ++ 
Sbjct: 143 GVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLST 202

Query: 186 SLDLDENSFLNQY-GKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGL 245
           +L L E+ F   + G+ +    R N+Y +C  P L LG+ PH+D   +TILL D +V GL
Sbjct: 203 NLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGL 262

Query: 246 QFLKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDP 305
           Q  K + W      P A +VN+GDQ +I SN  +KS  HRV+ NS++ER+SLA FY P  
Sbjct: 263 QVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKS 322

Query: 306 QKEIEPLEKLINETHPRLY 317
              I+PL++L++  +P LY
Sbjct: 323 DIPIQPLQELVSTHNPPLY 341

[Close]

BLAST of MC06g0564 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.5e-53
Identity = 114/350 (32.57%), Postives = 186/350 (53.14%), Query Frame = 0


Query: 3   GTNPTGSVQDVAS-----KGEVPERYIHKECDRGALDAPL---MEAPVIDIGLLSSPSNT 62
           G+ P  +VQ +A         +PERYI  E     +       M  P+ID+  L  P ++
Sbjct: 8   GSLPVPNVQALAEICNDPDEHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSS 67

Query: 63  GPELEKLRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGY 122
             E  KLR     WG F  INHG+  E +  +++    FF+ P++ K ++++  + +EGY
Sbjct: 68  EEECVKLRSACQYWGFFLLINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGY 127

Query: 123 GNDMIFSNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILK 182
           G   +FS  Q LDW D LYL V P +SR  ++WPT+P  FR+ +  Y++  K L+  + +
Sbjct: 128 GQSFVFSEDQKLDWADMLYLHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFE 187

Query: 183 AMARSLDLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEV 242
            MA+++     S L+ + ++ +   R  +Y  CR  + V+G+ PH+D   +T+LL+   V
Sbjct: 188 FMAKAVGAKPESLLDLFEEQPR-GLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNV 247

Query: 243 EGLQFLKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYL 302
           +GLQ  K  +W++      AL+ N+GD  EI SNG F+S  HR + N  +ERIS A+F+ 
Sbjct: 248 QGLQIKKDGKWFSIDAPNGALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHY 307

Query: 303 PDPQKEIEPLEKLINETHPRLYRTVK--NFVGLFFQYYQQGQRPREAAKI 343
           P     I PL + + +   + YR++   +F+   F     G+   E  K+
Sbjct: 308 PSENMVISPLPEFVKDGKVK-YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

[Close]

BLAST of MC06g0564 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.7e-52
Identity = 110/312 (35.26%), Postives = 168/312 (53.85%), Query Frame = 0


Query: 17  GEVPERYIHKECDRGAL------DAPLMEAPVIDIGLLSSPSNT--GPELEKLRHGLHSW 76
           G +P RY+     R  +      +      P+ID+G L +   T     L+++       
Sbjct: 23  GAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLTLQAKTLDEISKACREL 82

Query: 77  GCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFSNQQILDW 136
           G FQ +NHGMSP+ +++ +   + FF LPME K  H+      EGYG+ +      ILDW
Sbjct: 83  GFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHANSPKTYEGYGSRLGVEKGAILDW 142

Query: 137 TDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLDLDENSFL 196
           +D  YL   P   + +  WP+ P   RE+L +Y   +  L E ++K ++++L L E+   
Sbjct: 143 SDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENLMKILSKNLGLQEDRLQ 202

Query: 197 NQYGKRVQLDA--RFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLKGNE-- 256
           N +G + +     R N+Y +C  P L LG+ PH+D   +TILL D++V  LQ ++G++  
Sbjct: 203 NAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLPDEQVASLQ-VRGSDDA 262

Query: 257 WWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEIEPL 316
           W      P A +VN+GDQ ++ SN I+KS  HRV+ N E ER+SLA FY P     IEPL
Sbjct: 263 WITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPENERLSLAFFYNPKGNVPIEPL 322

[Close]

BLAST of MC06g0564 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 703 bits (1815), Expect = 4.16e-250
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
           KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

[Close]

BLAST of MC06g0564 vs. NCBI nr
Match: XP_038878494.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 604 bits (1558), Expect = 9.79e-217
Identity = 296/342 (86.55%), Postives = 320/342 (93.57%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGAL+APLM APVIDIGL SS    GPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFSS----GPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQAINHGMS EFL+EVRQ+AK FF LPME+KLK+SRE D+MEGYGNDM+ 
Sbjct: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVL 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           S+QQILDWTDRLYLTVCP++SRRFKYWPTNPERFREV+ EYTANVKL+SEKILKAMARSL
Sbjct: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDE+SFLNQYG+R+QLDARFNFY RCRNP+LVLGVKPHADGSAITILLQDKEVEGLQF+
Sbjct: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
           KGNEW+NAPIIP ALLVNVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD  KE
Sbjct: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLINET PRLY+TVKNFVGL+F+YYQQGQRP EAA+I
Sbjct: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 337

[Close]

BLAST of MC06g0564 vs. NCBI nr
Match: XP_022987763.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima])

HSP 1 Score: 595 bits (1533), Expect = 7.58e-213
Identity = 288/342 (84.21%), Postives = 317/342 (92.69%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIH E DRGALDAPLM APVID+ LLSSPS +GPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+EVR++AK FF LPME+K K+SREE+++EGYGNDMI 
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFREVLHEYTANVKLLSEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
            GNEW+NAPI+P ALLVNVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLI+ET PRLY++VKNFVGL+FQYYQQGQRP EAA+I
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of MC06g0564 vs. NCBI nr
Match: XP_022960723.1 (protein SRG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 592 bits (1525), Expect = 2.53e-211
Identity = 286/342 (83.63%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+E+R++AK FF LPME+K K+SREED++EGYGNDMI 
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFR VLHEYTANVKLLSEKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
            GNEW+NAPI+P ALL+NVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLI+ET PRLY+TVKNFVGL+FQYYQQGQRP EAA+I
Sbjct: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

[Close]

BLAST of MC06g0564 vs. NCBI nr
Match: XP_023516521.1 (protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590 bits (1520), Expect = 7.25e-211
Identity = 287/342 (83.92%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQ VASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 1   MAGTNPSGTVQGVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSKSGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+EVR++AK FF LPME+K K+SREE+++EGYGNDMI 
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFREVLHEYTANVKLLSEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
            GNEW+NAPI+P ALLVNVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWKKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLI+ET PRLY+TVKNFVGL+FQYYQQGQRP EAA+I
Sbjct: 301 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of MC06g0564 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 703 bits (1815), Expect = 2.01e-250
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
           KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

[Close]

BLAST of MC06g0564 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 3.67e-213
Identity = 288/342 (84.21%), Postives = 317/342 (92.69%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIH E DRGALDAPLM APVID+ LLSSPS +GPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+EVR++AK FF LPME+K K+SREE+++EGYGNDMI 
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFREVLHEYTANVKLLSEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
            GNEW+NAPI+P ALLVNVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLI+ET PRLY++VKNFVGL+FQYYQQGQRP EAA+I
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of MC06g0564 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 1.22e-211
Identity = 286/342 (83.63%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+E+R++AK FF LPME+K K+SREED++EGYGNDMI 
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFR VLHEYTANVKLLSEKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
            GNEW+NAPI+P ALL+NVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLEKLI+ET PRLY+TVKNFVGL+FQYYQQGQRP EAA+I
Sbjct: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

[Close]

BLAST of MC06g0564 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 1.66e-209
Identity = 283/342 (82.75%), Postives = 315/342 (92.11%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MA TNP+G+VQDVASKGEVPERYIHKE DRGA +APLM APVIDI LLSS S +GPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQAINHGM+ E+L+EVRQ+ K FF L ME+KLK+ +EE +MEGYGNDMI 
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180
           SNQQILDWTDRLYLTV P +SRRFKYWPTNP+RFREV+ EYT NVKL+SEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDE+SFLNQYG++V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQDKEVEGLQF+
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300
           K NEW+NAPI+PDALLVNVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342
           IEPLE+LINE+ PRLY++VKNFVGL+F+YYQQGQRP EAA+I
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

[Close]

BLAST of MC06g0564 vs. ExPASy TrEMBL
Match: A0A6J1JB89 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 581 bits (1498), Expect = 1.90e-207
Identity = 288/366 (78.69%), Postives = 317/366 (86.61%), Query Frame = 0


Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIH E DRGALDAPLM APVID+ LLSSPS +GPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120
           LRHGL SWGCFQ +NHGMS EFL+EVR++AK FF LPME+K K+SREE+++EGYGNDMI 
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFR------------------------E 180
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFR                        E
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRYFSQTFTIFRRICRNELIRDEHCRE 180

Query: 181 VLHEYTANVKLLSEKILKAMARSLDLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGV 240
           VLHEYTANVKLLSEKILKAMA SLDL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGV
Sbjct: 181 VLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGV 240

Query: 241 KPHADGSAITILLQDKEVEGLQFLKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVH 300
           KPHADGSAITILLQD+EVEGLQFL GNEW+NAPI+P ALLVNVGDQ EITSNGIFKS VH
Sbjct: 241 KPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVH 300

Query: 301 RVLTNSERERISLAVFYLPDPQKEIEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRP 342
           RVLTNSERERISLAVFYLPD +KEIEPLEKLI+ET PRLY++VKNFVGL+FQYYQQGQRP
Sbjct: 301 RVLTNSERERISLAVFYLPDSEKEIEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRP 360

[Close]

BLAST of MC06g0564 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.2e-107
Identity = 184/341 (53.96%), Postives = 244/341 (71.55%), Query Frame = 0


Query: 9   SVQDVASKGE-VPERYIHKECDRGALD-----APLMEAPVIDIGLLSSPSNTG-PELEKL 68
           +VQ+V + GE +PERY+H     G +       P M+ P ID+ LL S S  G  EL KL
Sbjct: 8   TVQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKL 67

Query: 69  RHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFS 128
              L +WG  Q +NHG++  FL+++ ++ K FFALP E+K K +RE D ++GYGNDMI  
Sbjct: 68  HSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILW 127

Query: 129 NQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLD 188
           + Q+LDW DRLY+T  PE+ R+  +WP  P  FRE LHEYT   +++ E+  KAMARSL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLE 187

Query: 189 LDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLK 248
           L+ENSFL+ YG+   LD RFN Y  C +P+ V+GVKPHADGSAIT+LL DK+V GLQF K
Sbjct: 188 LEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQK 247

Query: 249 GNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEI 308
             +W+ API+PD +L+NVGDQ EI SNGI+KS VHRV+TN E+ERIS+A F +P   KEI
Sbjct: 248 DGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEI 307

Query: 309 EPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 343
           +P+ +L++E  PRLY+TVK +V L+F+YYQQG+RP EAA I
Sbjct: 308 QPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

[Close]

BLAST of MC06g0564 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 2.0e-101
Identity = 176/341 (51.61%), Postives = 237/341 (69.50%), Query Frame = 0


Query: 9   SVQDVASKGE-VPERYIHKECDRGALD-----APLMEAPVIDIGLLSSPSNTG-PELEKL 68
           +VQ+V + G+ +PERY+H     G         P M+ P ID+ LL S S  G  E++KL
Sbjct: 8   TVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKL 67

Query: 69  RHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFS 128
              L +WG  Q +NHG++  FL+++ ++ K FFALP E+K K +RE   ++GYGNDMI S
Sbjct: 68  HSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILS 127

Query: 129 NQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLD 188
           + Q+LDW DRL+LT  PE+ R+ K+WP  P  F E L EYT   ++L EK  KAMARSL+
Sbjct: 128 DNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLE 187

Query: 189 LDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLK 248
           L+EN FL  YG+   +++RFNF+  C  P+ V+G+KPHADGSAIT+LL DK+VEGLQFLK
Sbjct: 188 LEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLK 247

Query: 249 GNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEI 308
             +W+ API+PD +L+ +GDQ EI SNGI+KS VHRV+TN E+ERIS+A F +P   KEI
Sbjct: 248 DGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEI 307

Query: 309 EPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 343
            P + L+ E  PRLY+TV  +V L ++YYQQG+R  EAA I
Sbjct: 308 HPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

[Close]

BLAST of MC06g0564 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 8.0e-98
Identity = 176/342 (51.46%), Postives = 238/342 (69.59%), Query Frame = 0


Query: 9   SVQDVASKGE-VPERYIHKEC--DRGA-LDA--PLMEAPVIDIGLLSSPSNTG-PELEKL 68
           +VQ+V + GE +PERY+      D G  L+A  P+M+ P ID+ LL SPS+ G  EL KL
Sbjct: 8   TVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKL 67

Query: 69  RHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFS 128
              L +WG  Q INHG++   L+++ ++ K F ALP E+K K++RE   ++GYGNDMI  
Sbjct: 68  HSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILW 127

Query: 129 NQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLD 188
           + Q+LDW DRLY+T  PE+ R+ K+WP  P  FRE LHEYT    L+  ++ KAMA SL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLE 187

Query: 189 LDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLK 248
           L+EN FL+  G+   +D RFN Y  C  P+ V+GV+PHAD SA T+LL DK VEGLQFLK
Sbjct: 188 LEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLK 247

Query: 249 GNEWWNAPII-PDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 308
             +W+ AP++  D +L+NVGDQ EI SNGI+KS VHRV+TN+E+ERIS+A F +P   KE
Sbjct: 248 DGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKE 307

Query: 309 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 343
           I+P++ L++E  PRLY+ VKN+V L  +YY QGQRP  A+ I
Sbjct: 308 IQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

[Close]

BLAST of MC06g0564 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.8e-97
Identity = 169/342 (49.42%), Postives = 229/342 (66.96%), Query Frame = 0


Query: 9   SVQDVASKGE-VPERYIHKECDRGALDAPL------MEAPVIDIGLLSSPSNTG-PELEK 68
           +VQ+V + GE +PERY++     G  D P       M+  +ID+ LL S S+ G  EL K
Sbjct: 8   TVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSK 67

Query: 69  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 128
           L   + +WG  Q +NHG+S   L+++ ++ K FF LP ++K K++RE    +G+GNDMI 
Sbjct: 68  LHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMIL 127

Query: 129 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 188
           S+ Q+LDW DRLYL   PE+ R+ K+WP NP  FRE LHEYT   +L+ EK  KA+ARSL
Sbjct: 128 SDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSL 187

Query: 189 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 248
           +L++N FL  +G+   L+ RFN Y  C  P+ VLG+KPH+DGSA T++L DK VEGLQFL
Sbjct: 188 ELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFL 247

Query: 249 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 308
           K  +W+ A I+P  +L+NVGD  E+ SNGI+KS VHRV+ N ++ERI +A F   D  KE
Sbjct: 248 KDGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKE 307

Query: 309 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 343
           I+PL  L++E  PRLY+ VK     FF YYQQG+RP EAA I
Sbjct: 308 IQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of MC06g0564 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.7e-69
Identity = 142/354 (40.11%), Postives = 209/354 (59.04%), Query Frame = 0


Query: 8   GSVQDV-----ASKGEVPERYIHKECDRGALDAPLM------EAPVIDIGLLSSPSNTG- 67
           G + DV     +   +VPER+I +E +RG + + L       + PVID+  LS P N   
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 68  -PELEKLRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGY 127
             E+ KL      WG FQ INHG+  E +E++ +VA  FF +P+E+K K+  E   ++GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 128 GNDMIFSNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILK 187
           G   IFS  Q LDW +   L V P + R  K WP+ P RF E L  Y+  ++ L +++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 188 AMARSLDLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDK-E 247
            +A SL L E  F   +G+ VQ   R N+Y  C +P+LVLG+ PH+DGSA+T+L Q K  
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNS 251

Query: 248 VEGLQFLKGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFY 307
             GLQ LK N W     +P+AL++N+GD  E+ SNG +KS  HR +TN E+ER+++  FY
Sbjct: 252 CVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFY 311

Query: 308 LPDPQKEIEPLEKLI-NETHPRLYRTVKNFVGLFFQYY----QQGQRPREAAKI 343
            P+ + EIEP+ +L+ +ET+P  YR+  +  G +  +Y     QG++  + AKI
Sbjct: 312 APNYEVEIEPMSELVDDETNPCKYRSYNH--GDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:

BLAST of MC06g0564 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80449	1.3e-57	37.07	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q39224	6.9e-54	33.64	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
Q9FFF6	2.0e-53	35.42	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
Q94LP4	4.5e-53	32.57	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9LY48	1.7e-52	35.26	Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1	[more]

BLAST of MC06g0564 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134811.1	4.16e-250	100.00	uncharacterized protein LOC111006992 [Momordica charantia]	[more]
XP_038878494.1	9.79e-217	86.55	probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida]	[more]
XP_022987763.1	7.58e-213	84.21	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima]	[more]
XP_022960723.1	2.53e-211	83.63	protein SRG1-like isoform X1 [Cucurbita moschata]	[more]
XP_023516521.1	7.25e-211	83.92	protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC06g0564 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYU1	2.01e-250	100.00	uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A6J1JKD6	3.67e-213	84.21	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1H9T6	1.22e-211	83.63	protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 ...	[more]
A0A5A7U2M0	1.66e-209	82.75	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00...	[more]
A0A6J1JB89	1.90e-207	78.69	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 OS=Cucurbita maxima...	[more]

BLAST of MC06g0564 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20400.1	4.2e-107	53.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	2.0e-101	51.61	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20550.1	8.0e-98	51.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	1.8e-97	49.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	2.7e-69	40.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 41..148 e-value: 7.6E-23 score: 81.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..294 e-value: 2.1E-18 score: 66.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..329 e-value: 1.4E-88 score: 299.4
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 9..333
None	No IPR available	PANTHER	PTHR47991:SF63	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 9..333
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..320
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 181..295 score: 12.165351

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0564.1	MC06g0564.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding