MC06g0526 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0526
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Replication termination factor 2
Location	MC06: 4345823 .. 4347891 (+)
RNA-Seq Expression	MC06g0526
Synteny	MC06g0526

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAAAAAATCCTAATGGTACTAAAAAAATTCCTAACTTTGTCTCGAATAAAAGATTTGGAAAAAATATTTTCAAATATTAAAATTTTGCACTATATAATATTTTGTTGATCGCTGGCGTCTGGACTTTGAAACATCTGGATTTTGGTTTCCTTTGTCCTCCCGTTTATCCCTCAAGTCGAAGAATCGTCGTCCATCTTCTTATTGTTATCCAAACCCTAGCTACGGAATTTGATTCTCGAAGATCATTCTGATTGTGAAGCCACGGAATTTTGTCACCATCTTCTTCGACTATCCAAACCCTAACCCAATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAAACCTAGCACGAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTCTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGGCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTGCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAACTGATCAGTGTTAAGAATTGCCTTGTTCTGTTTATGGCGATTCTCCTTATATTGTTTATGTCTCGTTGTTGCTAATTGATGTAAGATATGGCCTATTAGTTTACATTCATTAACGACTGTTGCTCTGTGGAACTGTTTTTCAATCTTAGATCAGTCCATGTTCTTGGAAGTCTTGCGCTGATCAATCCCGTTTCTTATCAACATCTGAGTTCATGTTTGAGGTCTTGAAAATAAACCCCCTGATGGCTGATGGAGTCTCTTCTTTGTATATTTTGAGAGTTAATTTCTTCTGTAGGAGGCGTACCTCTTTCTTTGTTGTGTTATATATCAATATCTGCTGAATATTATATGCTGAGATATCCCATAAGGTTTCCTTTGTGTGTGTCTGATGGGATTATGGCAAAGTTTGTACTTTGTTCAGATGTATTGTTCTAGAATTCTTTAACTTCTTGTGTTTCTGACTTCAGATGTATTGTTCTAAGAACTCTTTAACTTCTCGTGTTTCTGACTTATCCCTATTCCCACATCATGATAAATTATAGCTACTTTATGTTGTCTTTATTGGATTATAAAGCTCTGTTGGAGCTGTGATCTGATTCAGCC

mRNA sequence

CTAAAAAAATCCTAATGGTACTAAAAAAATTCCTAACTTTGTCTCGAATAAAAGATTTGGAAAAAATATTTTCAAATATTAAAATTTTGCACTATATAATATTTTGTTGATCGCTGGCGTCTGGACTTTGAAACATCTGGATTTTGGTTTCCTTTGTCCTCCCGTTTATCCCTCAAGTCGAAGAATCGTCGTCCATCTTCTTATTGTTATCCAAACCCTAGCTACGGAATTTGATTCTCGAAGATCATTCTGATTGTGAAGCCACGGAATTTTGTCACCATCTTCTTCGACTATCCAAACCCTAACCCAATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAAACCTAGCACGAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTCTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGGCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTGCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAACTGATCAGTGTTAAGAATTGCCTTGTTCTGTTTATGGCGATTCTCCTTATATTGTTTATGTCTCGTTGTTGCTAATTGATGTAAGATATGGCCTATTAGTTTACATTCATTAACGACTGTTGCTCTGTGGAACTGTTTTTCAATCTTAGATCAGTCCATGTTCTTGGAAGTCTTGCGCTGATCAATCCCGTTTCTTATCAACATCTGAGTTCATGTTTGAGGTCTTGAAAATAAACCCCCTGATGGCTGATGGAGTCTCTTCTTTGTATATTTTGAGAGTTAATTTCTTCTGTAGGAGGCGTACCTCTTTCTTTGTTGTGTTATATATCAATATCTGCTGAATATTATATGCTGAGATATCCCATAAGGTTTCCTTTGTGTGTGTCTGATGGGATTATGGCAAAGTTTGTACTTTGTTCAGATGTATTGTTCTAGAATTCTTTAACTTCTTGTGTTTCTGACTTCAGATGTATTGTTCTAAGAACTCTTTAACTTCTCGTGTTTCTGACTTATCCCTATTCCCACATCATGATAAATTATAGCTACTTTATGTTGTCTTTATTGGATTATAAAGCTCTGTTGGAGCTGTGATCTGATTCAGCC

Coding sequence (CDS)

ATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAAACCTAGCACGAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTCTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGGCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTGCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAACTGA

Protein sequence

MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLNGKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKISLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVYASIFTSSRKSDFKETYSCRSLPLGRN

Homology

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BLAST of MC06g0526 vs. ExPASy Swiss-Prot
Match: Q3T1J8 (Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.4e-34
Identity = 98/271 (36.16%), Postives = 146/271 (53.87%), Query Frame = 0


Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEVLRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H++ +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  TSHIRSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V +L+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKRRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLA--SSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVA 351
            KK K  E      +S D A  S   +GK    +    EK S+   R    +G    KV 
Sbjct: 199 TKKPKTAESASKLGISQDSAGPSKAKAGKSEEADPDPREKKSSLAPRGTASNGSASGKVG 258

Query: 352 ANN-GAVKRFKAADMAPPNATKEVYASIFTS 367
               GA+KR  A      +   E Y SIFTS
Sbjct: 259 KPPCGALKRSIA-----DSEESETYKSIFTS 284

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BLAST of MC06g0526 vs. ExPASy Swiss-Prot
Match: Q99K95 (Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 9.9e-33
Identity = 98/272 (36.03%), Postives = 145/272 (53.31%), Query Frame = 0


Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H++ +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIRSIKNVTELRLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V +L++RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKKRMEERRLRAKLEKK 198

Query: 292 LKKVKNG-EVGMNGDVSVDLA--SSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKV 351
            KK K   E       + D A  S   SGK    +    EK S    R    +G    KV
Sbjct: 199 TKKPKTATECASKPGTTQDSAGPSKVKSGKPEEADPDPREKKSTPAPRGAATNGSASGKV 258

Query: 352 AANN-GAVKRFKAADMAPPNATKEVYASIFTS 367
                GA+KR  A      +   E Y SIFTS
Sbjct: 259 GKPPCGALKRSIA-----DSEESETYKSIFTS 285

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BLAST of MC06g0526 vs. ExPASy Swiss-Prot
Match: A5GFW7 (Replication termination factor 2 OS=Sus scrofa OX=9823 GN=RTF2 PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-32
Identity = 95/281 (33.81%), Postives = 149/281 (53.02%), Query Frame = 0


Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSSEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E    +  G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELRLSDNPAWEGDKGSTKGDKHDDLQRARFICPVVGLEMNGRHRFCYLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+VA+L+ RMEE + ++K  K+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVAMLQTRMEERRLRAKLGKK 198

Query: 292 LKKVKNGEVGMNGDVSVDL--ASSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVA 351
            KK K  E     D+S +    S   +GK     + + EK ++   R+    G    K  
Sbjct: 199 TKKPKAAESVSKSDISEEAPGPSKMKAGKPEETSLDSREKKTSSAPRSAAAHGSSSGKAG 258

Query: 352 ANNGAVKRFKAADMAPPNATKEVYASIFT--SSRKSDFKET 376
                  +   AD    +   E Y S+FT  SS K   +E+
Sbjct: 259 KPVCGAPKRSIAD----SGESEAYKSLFTTHSSAKRSKEES 295

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BLAST of MC06g0526 vs. ExPASy Swiss-Prot
Match: Q5R9P9 (Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.8e-32
Identity = 99/288 (34.38%), Postives = 148/288 (51.39%), Query Frame = 0


Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V VL+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLASSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAAN 351
            KK K  E     DVS +             +VK  +   A L+  E+         A N
Sbjct: 199 TKKPKAAESVSKPDVSEEAPGPS--------KVKTGKPEEASLDSREKKTNLAPKSTATN 258

Query: 352 NGAVKRFKAADMAPPNATK---------EVYASIFT--SSRKSDFKET 376
             +  +   A   P  ATK         E Y S+FT  SS K   +E+
Sbjct: 259 ESSSGK---AGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEES 295

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BLAST of MC06g0526 vs. ExPASy Swiss-Prot
Match: Q9BY42 (Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3)

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-31
Identity = 98/288 (34.03%), Postives = 148/288 (51.39%), Query Frame = 0


Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +++NG++E+V VL+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIMLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLASSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAAN 351
            KK K  E     DVS +             +VK  +   A L+  E+         A N
Sbjct: 199 TKKPKAAESVSKPDVSEEAPGPS--------KVKTGKPEEASLDSREKKTNLAPKSTAMN 258

Query: 352 NGAVKRFKAADMAPPNATK---------EVYASIFT--SSRKSDFKET 376
             +  +   A   P  ATK         E Y S+FT  SS K   +E+
Sbjct: 259 ESSSGK---AGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEES 295

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BLAST of MC06g0526 vs. NCBI nr
Match: XP_022135450.1 (protein RTF2 homolog [Momordica charantia])

HSP 1 Score: 755 bits (1950), Expect = 6.53e-275
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

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BLAST of MC06g0526 vs. NCBI nr
Match: XP_038879920.1 (replication termination factor 2 [Benincasa hispida])

HSP 1 Score: 658 bits (1697), Expect = 2.50e-236
Identity = 336/388 (86.60%), Postives = 358/388 (92.27%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           MHPKN+ RLQIF+HS DLQIQS+I+NL ++ AQTLEDLKFSLLP+TLA R ASSFYFTLN
Sbjct: 1   MHPKNEHRLQIFLHSLDLQIQSQIVNLPQSPAQTLEDLKFSLLPETLASRVASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DST + SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLPDSTTIPSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSVIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKS--KEKRLKKVKNGEVGMNGDVSVDLA 300
           CHAEF +RDK VINGSEEEV  +RERMEEEKSKS  KEK+ KKV+N E G+NGD+SVD A
Sbjct: 241 CHAEFTNRDKFVINGSEEEVEEMRERMEEEKSKSRSKEKKTKKVRNEEGGINGDMSVDFA 300

Query: 301 SSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKE 360
           +SRLSGKKHGIEVKA EKVSAK ER+ERPDGG+QVKVAA+NGAVKRFKAADM P NATKE
Sbjct: 301 ASRLSGKKHGIEVKALEKVSAKPERHERPDGGVQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 388

[Close]

BLAST of MC06g0526 vs. NCBI nr
Match: XP_022933589.1 (protein RTF2 homolog [Cucurbita moschata])

HSP 1 Score: 653 bits (1685), Expect = 1.56e-234
Identity = 332/386 (86.01%), Postives = 353/386 (91.45%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI+NLA+TSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS +PGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKVAA+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. NCBI nr
Match: KAG7035670.1 (Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653 bits (1684), Expect = 2.22e-234
Identity = 333/386 (86.27%), Postives = 352/386 (91.19%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI+NLA+TSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKVAA+NG VKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. NCBI nr
Match: KAG6587836.1 (Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 652 bits (1683), Expect = 3.15e-234
Identity = 332/386 (86.01%), Postives = 352/386 (91.19%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI+NLA+TSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKVAA+NG VKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. ExPASy TrEMBL
Match: A0A6J1C136 (Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 PE=3 SV=1)

HSP 1 Score: 755 bits (1950), Expect = 3.16e-275
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. ExPASy TrEMBL
Match: A0A6J1F067 (Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 PE=3 SV=1)

HSP 1 Score: 653 bits (1685), Expect = 7.57e-235
Identity = 332/386 (86.01%), Postives = 353/386 (91.45%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI+NLA+TSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS +PGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKVAA+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. ExPASy TrEMBL
Match: A0A5A7SWS4 (Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00470 PE=3 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 6.21e-234
Identity = 336/388 (86.60%), Postives = 356/388 (91.75%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           MHPKNQ RLQIF+HSPDLQIQSKI++L +TSA+TLEDLKFSLL +TLA R ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK--EKRLKKVKNGEVGMNGDVSVDLA 300
           CH EF +RDK VINGSEEEV  +RERMEEEKSKSK  EK+ KKV+NGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKE 360
           +SRLSGKKHGIE++  EKVSAK ER+ER DGG QVKVAA+NGAVKRFKAADM P NATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. ExPASy TrEMBL
Match: A0A1S3B9F3 (Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 6.21e-234
Identity = 336/388 (86.60%), Postives = 356/388 (91.75%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           MHPKNQ RLQIF+HSPDLQIQSKI++L +TSA+TLEDLKFSLL +TLA R ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK--EKRLKKVKNGEVGMNGDVSVDLA 300
           CH EF +RDK VINGSEEEV  +RERMEEEKSKSK  EK+ KKV+NGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKE 360
           +SRLSGKKHGIE++  EKVSAK ER+ER DGG QVKVAA+NGAVKRFKAADM P NATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. ExPASy TrEMBL
Match: A0A6J1KUM3 (Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=3 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 5.93e-232
Identity = 329/386 (85.23%), Postives = 350/386 (90.67%), Query Frame = 0


Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60
           M+PKNQ RLQ FV SPDLQIQSKI+NLA+TSAQ+L DLK SLL    A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVRSPDLQIQSKIVNLAQTSAQSLADLKLSLLLNAPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKESLV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  N IS  RFQCPITGLEFNGKYKFFALR CGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNEISEARFQCPITGLEFNGKYKFFALRACGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+K+KEK+ KKV+ GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKAKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  K+SAK E++ERPD G+QVKVAA+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKISAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

[Close]

BLAST of MC06g0526 vs. TAIR 10
Match: AT5G58020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 (InterPro:IPR006735); Has 385 Blast hits to 385 proteins in 189 species: Archae - 0; Bacteria - 0; Metazoa - 153; Fungi - 117; Plants - 50; Viruses - 0; Other Eukaryotes - 65 (source: NCBI BLink). )

HSP 1 Score: 396.7 bits (1018), Expect = 2.1e-110
Identity = 226/383 (59.01%), Postives = 267/383 (69.71%), Query Frame = 0


Query: 8   RLQIFVHSPDLQIQSKILNLARTSAQTLEDLK-FSLLPQTLAPRSASSFYFTLNGKPLLD 67
           R QIFV SPD Q   K++ L    AQ+L  L   + L ++    S S+   TL+GK L  
Sbjct: 4   RRQIFVKSPDCQ---KVVALQLDPAQSLLTLSGITSLLESSQRISFSACSITLDGKLLNG 63

Query: 68  STPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK 127
           ST +  S +  +S L L  R+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK
Sbjct: 64  STRIQVSKLPSVSMLTLFPRLRGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK 123

Query: 128 WLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKISLSAIP 187
           WLNCALSNEPL EPCVID LGN+FNKE LV ALL K+LPK F ++KGLKDM+ I L+ + 
Sbjct: 124 WLNCALSNEPLAEPCVIDLLGNLFNKEVLVHALLSKRLPKQFSYIKGLKDMVNIKLTPVA 183

Query: 188 GTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLVCHAEFK 247
           G++  S   +  +FQCP++GLEFNGKYKFFALR CGHV+SAKALKEVKSSSCLVCHA+ K
Sbjct: 184 GSDGSSQDTTSAQFQCPVSGLEFNGKYKFFALRGCGHVMSAKALKEVKSSSCLVCHADVK 243

Query: 248 DRDKLVINGSEEEVAVLRERMEEEKSKSKEKR--LKKVKNGEVGMNGDVSVDLASSRLSG 307
           D DK+VING+EEEV +LRERMEEEK+K +EK+   KK KNG       V  D        
Sbjct: 244 DSDKIVINGTEEEVDLLRERMEEEKAKLREKKGVSKKSKNGAA-----VVAD-------- 303

Query: 308 KKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVYASIF 367
                         AK+ + +  DG +      N   VK+FKAAD  P NATKEVYAS+F
Sbjct: 304 ------------TGAKVAKRQIEDGNVN----GNGITVKKFKAADKVPVNATKEVYASLF 354

Query: 368 TSS-RKSDFKETYSCRSLPLGRN 387
           TSS +KSDF+ETYSCRSLPLGRN
Sbjct: 364 TSSKKKSDFRETYSCRSLPLGRN 354

The following BLAST results are available for this feature:

BLAST of MC06g0526 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3T1J8	1.4e-34	36.16	Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1	[more]
Q99K95	9.9e-33	36.03	Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1	[more]
A5GFW7	1.3e-32	33.81	Replication termination factor 2 OS=Sus scrofa OX=9823 GN=RTF2 PE=3 SV=1	[more]
Q5R9P9	3.8e-32	34.38	Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1	[more]
Q9BY42	1.1e-31	34.03	Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3	[more]

BLAST of MC06g0526 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135450.1	6.53e-275	100.00	protein RTF2 homolog [Momordica charantia]	[more]
XP_038879920.1	2.50e-236	86.60	replication termination factor 2 [Benincasa hispida]	[more]
XP_022933589.1	1.56e-234	86.01	protein RTF2 homolog [Cucurbita moschata]	[more]
KAG7035670.1	2.22e-234	86.27	Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6587836.1	3.15e-234	86.01	Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of MC06g0526 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C136	3.16e-275	100.00	Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 ...	[more]
A0A6J1F067	7.57e-235	86.01	Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 P...	[more]
A0A5A7SWS4	6.21e-234	86.60	Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B9F3	6.21e-234	86.60	Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV...	[more]
A0A6J1KUM3	5.93e-232	85.23	Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=...	[more]

BLAST of MC06g0526 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58020.1	2.1e-110	59.01	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 253..280
None	No IPR available	PFAM	PF04641	Rtf2	coord: 90..370 e-value: 3.2E-72 score: 243.1
None	No IPR available	PANTHER	PTHR12775:SF1	BNACNNG39770D PROTEIN	coord: 7..300 coord: 332..386
IPR006735	Replication termination factor 2	PANTHER	PTHR12775	PROTEIN C20ORF43 HOMOLOG	coord: 7..300 coord: 332..386
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 9..90 score: 9.351744
IPR027799	Replication termination factor 2, RING-finger	CDD	cd16653	RING-like_Rtf2	coord: 200..245 e-value: 8.04194E-27 score: 99.2375

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0526.1	MC06g0526.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902979	mitotic DNA replication termination
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding