MC06g0521 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0521 vs. ExPASy Swiss-Prot
Match: Q56X72 (Reticulon-like protein B21 OS=Arabidopsis thaliana OX=3702 GN=RTNLB21 PE=2 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 7.6e-85
Identity = 214/503 (42.54%), Postives = 304/503 (60.44%), Query Frame = 0

Query: 105 RKLRSEGNEENSVAIGRIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTID 164
           R L S  + + S ++  +       +K++SE      ++T   T  +++K++S+   T  
Sbjct: 5   RSLSSSDSNDKSPSVSVV------AKKARSESVEGIEKKT---TPGRVKKIRSEVCTT-- 64

Query: 165 NDNAKAIIVVAGDGFGANLEHLKN-PESESNEVEKGIHGSVKEIEKPQVENESSCREFGV 224
                  IV AG+     L  + + P   S + +      V  IE  ++  E   +EFGV
Sbjct: 65  -------IVKAGEFDSVALRKVNSLPSPNSEKSDTKTEQEVTIIENSKIPEE--VKEFGV 124

Query: 225 CH---IPIKSAQNVSIDDSDVKDENEGD-EGEESEEEDIEIEKESFDIKEINLPEPKKVV 284
           C    +  KS +N  ID+ D +  ++ D E +  EEE+ E+EK+S D+KEIN+ +  +V 
Sbjct: 125 CQEMIVSAKSNENEQIDNGDQEIGDQDDYEEDGDEEEEREVEKKSVDVKEINVAKENRVG 184

Query: 285 N-EVKRFHQI----------YQKQSPPINKHST-VYQNLSKPTTTPDEY----HSFQEKS 344
             E+K+F Q            +K SPP+ K +T VY      T++ D +     +F    
Sbjct: 185 GVEIKKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPNSTSSTDRFAEQEDNFTHSQ 244

Query: 345 NKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGLFYLAATFLY 404
           +KLQSLVDLVMW+DVSRS  VFG G+F+IISSSYA D+N SFISVV+Y+GL YL   F+ 
Sbjct: 245 SKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLIYLGLMFVL 304

Query: 405 RSIICRGAIDTEHVRHV-VGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPATTMKLAVLL 464
           +S+I RG ++ E  + V V EE+   +L+ ++PYLNE L +LRALF GDP+TT+K+ V+L
Sbjct: 305 KSLIHRGMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPSTTLKMGVVL 364

Query: 465 FALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKG 524
           F LARCGSSIT+W +AK+GF G FT+PK+  SYS+  +AY  FW+RRF DAWESC+HKK 
Sbjct: 365 FVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFWMRRFRDAWESCNHKKA 424

Query: 525 VAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSIL-TDDWVEEDEVEEVGDTCQASIG 581
           VA+A+F LVWNLSSV AR+WAAFML VAFRYYQ  ++ T D  ++DE +   +  +A   
Sbjct: 425 VALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKMIWTTDQADDDEDDNEEE--EAEEE 484

BLAST of MC06g0521 vs. ExPASy Swiss-Prot
Match: Q6DR04 (Reticulon-like protein B17 OS=Arabidopsis thaliana OX=3702 GN=RTNLB17 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.7e-34
Identity = 107/302 (35.43%), Postives = 164/302 (54.30%), Query Frame = 0

Query: 256 EDIEIEKESFDIKEI-NLPEPKKVVNEVKRFHQIYQKQSPPINKHSTVYQNLSKPTTTPD 315
           E +E ++ +  I+EI  LP  K+  N   R  +  Q  +PP+   S +      P T   
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTN--GRPKKDKQSSAPPLCSSSDL------PNTCQS 167

Query: 316 EYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGL 375
           + +   E       + DLVMW+DV++S   FG G    +SS +AK +N S  S VS LGL
Sbjct: 168 DLNLIGE------IISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGL 227

Query: 376 FYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPAT 435
             L  +FL  ++  R   DT+   H V E++ +   + VLP  N F+ K   LF G+P+ 
Sbjct: 228 VLLCGSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSM 287

Query: 436 TMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAW 495
           T+K+   L   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++   +    R G+AW
Sbjct: 288 TLKVTPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAW 347

Query: 496 ESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSI-LTDDWVEEDEVEEVG 555
             CSHKK +A +     WNL+S+  RI+A F++ V FRY +Q++ LT + VE  E E+  
Sbjct: 348 GVCSHKKILAGSAVTAFWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEEVEPVENEQEE 394

BLAST of MC06g0521 vs. ExPASy Swiss-Prot
Match: Q8LDS3 (Reticulon-like protein B18 OS=Arabidopsis thaliana OX=3702 GN=RTNLB18 PE=2 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 1.5e-32
Identity = 100/303 (33.00%), Postives = 163/303 (53.80%), Query Frame = 0

Query: 274 EPKKVVNEVKRFHQIYQKQ--SPPINKHSTVYQNLSKPTTTPDEYHSF--QEKSNKLQSL 333
           EP  VV + K+ +Q  QK+       KHS+V   L+ P+ + D+       +     +++
Sbjct: 137 EPIVVVTDEKKQNQRKQKKLGRSKKEKHSSV-PLLASPSPSSDQPQDVCQGDLERIRENI 196

Query: 334 VDLVMWKDVSRSAFVFGIGSFVIISSSY-AKDINISFISVVSYLGLFYLAATFLYRSIIC 393
            DL+MW+DV++S   FG G    +S+ + AK  N S  S +SYLGL +L  +FL  ++  
Sbjct: 197 SDLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLFLGVSFLSNTL-- 256

Query: 394 RGAIDTEHVRHV-VGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALAR 453
           R  +  E  R + + E++ + + + +LP  N  + K   LF G+PA T+K+A  +   A 
Sbjct: 257 RQRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMTLKVAPFVLMGAE 316

Query: 454 CGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAI 513
            G  IT+W++  +GFF  FT+PK+ S Y+SQL    +   RRF +AW  C+HKK VA + 
Sbjct: 317 YGYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKVECAQRRFVEAWGVCTHKKFVAGSA 376

Query: 514 FVLVWNLSSVVARIWAAFMLFVAFRYYQQSILTDDWVEEDEVEEVGDTCQASIGKEKHGS 571
               WNL+S+  R  A F++ V  RY +Q++  D   EE++ ++     +     E   +
Sbjct: 377 VTAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLDSEDEEEKKQQEKTHPEQQKSPEDKST 436

BLAST of MC06g0521 vs. ExPASy Swiss-Prot
Match: Q99P72 (Reticulon-4 OS=Mus musculus OX=10090 GN=Rtn4 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 6.6e-04
Identity = 46/169 (27.22%), Postives = 81/169 (47.93%), Query Frame = 0

Query: 328  SLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSII 387
            S+VDL+ W+D+ ++  VFG   F+++S +       S +SV +Y+ L  L+ T  +R  I
Sbjct: 974  SVVDLLYWRDIKKTGVVFGASLFLLLSLTV-----FSIVSVTAYIALALLSVTISFR--I 1033

Query: 388  CRGAI------DTEH-VRHVVGEEEAI---WVLKF---VLPYLNEFLLKLRALF-CGDPA 447
             +G I      D  H  R  +  E AI    V K+    L ++N  + +LR LF   D  
Sbjct: 1034 YKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLV 1093

Query: 448  TTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAY 483
             ++K AVL++     G+      +       +F++P +   + +Q+  Y
Sbjct: 1094 DSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHY 1135

BLAST of MC06g0521 vs. ExPASy Swiss-Prot
Match: Q9NQC3 (Reticulon-4 OS=Homo sapiens OX=9606 GN=RTN4 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 8.6e-04
Identity = 45/169 (26.63%), Postives = 75/169 (44.38%), Query Frame = 0

Query: 328  SLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSII 387
            S+VDL+ W+D+ ++  VFG   F+++S +       S +SV +Y+ L  L+ T  +R  I
Sbjct: 1004 SVVDLLYWRDIKKTGVVFGASLFLLLSLTV-----FSIVSVTAYIALALLSVTISFR--I 1063

Query: 388  CRGAI------DTEHVRHVVGEEEAIWVLKFVLPYLNEFL-------LKLRALF-CGDPA 447
             +G I      D  H      E E     + V  Y N  L        +LR LF   D  
Sbjct: 1064 YKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLV 1123

Query: 448  TTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAY 483
             ++K AVL++     G+      +       +F+VP +   + +Q+  Y
Sbjct: 1124 DSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHY 1165

BLAST of MC06g0521 vs. NCBI nr
Match: XP_022135248.1 (reticulon-like protein B21 isoform X1 [Momordica charantia])

HSP 1 Score: 1117 bits (2888), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST
Sbjct: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG
Sbjct: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
           RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG
Sbjct: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180

Query: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240
           ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD
Sbjct: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240

Query: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300
           VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS
Sbjct: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300

Query: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360
           TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD
Sbjct: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360

Query: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420
           INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF
Sbjct: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420

Query: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480
           LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA
Sbjct: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480

Query: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540
           AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT
Sbjct: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540

Query: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF
Sbjct: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583

BLAST of MC06g0521 vs. NCBI nr
Match: XP_022135249.1 (reticulon-like protein B21 isoform X2 [Momordica charantia])

HSP 1 Score: 1105 bits (2858), Expect = 0.0
Identity = 580/583 (99.49%), Postives = 581/583 (99.66%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST
Sbjct: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG
Sbjct: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
           RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG
Sbjct: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180

Query: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240
           ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD
Sbjct: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240

Query: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300
           VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS
Sbjct: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300

Query: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360
           TVYQNLSKPTT  +EYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD
Sbjct: 301 TVYQNLSKPTT--NEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360

Query: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420
           INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF
Sbjct: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420

Query: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480
           LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA
Sbjct: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480

Query: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540
           AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT
Sbjct: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540

Query: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF
Sbjct: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 581

BLAST of MC06g0521 vs. NCBI nr
Match: XP_023515635.1 (reticulon-like protein B21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 806 bits (2082), Expect = 9.74e-289
Identity = 459/597 (76.88%), Postives = 499/597 (83.58%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTRAM KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMPKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDSIRTI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSIRTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
           Q VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 QKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K  P            +KPT T +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPGP----------TSTKPTFTSNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSYAKDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYAKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDE---VEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E   VEE   TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEVEVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 565

BLAST of MC06g0521 vs. NCBI nr
Match: XP_022988563.1 (reticulon-like protein B21 isoform X1 [Cucurbita maxima])

HSP 1 Score: 803 bits (2074), Expect = 1.49e-287
Identity = 456/595 (76.64%), Postives = 497/595 (83.53%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTRAM KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDS RTI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
             VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 LKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K  P            +KPT T +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPGP----------TSTKPTFTSNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSY KDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYTKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDEVEEVGD-TCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E EE G+ TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEEEEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 563

BLAST of MC06g0521 vs. NCBI nr
Match: XP_022921916.1 (reticulon-like protein B21 isoform X1 [Cucurbita moschata])

HSP 1 Score: 803 bits (2073), Expect = 2.19e-287
Identity = 457/596 (76.68%), Postives = 497/596 (83.39%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTR M KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRVMPKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDSI TI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSISTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLVVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
           Q VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 QKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K  P            +KPT T +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPGP----------TSTKPTFTSNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSYAKDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYAKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDE--VEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E  VEE   TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEEVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 564

BLAST of MC06g0521 vs. ExPASy TrEMBL
Match: A0A6J1C4A7 (Reticulon-like protein OS=Momordica charantia OX=3673 GN=LOC111007258 PE=4 SV=1)

HSP 1 Score: 1117 bits (2888), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST
Sbjct: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG
Sbjct: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
           RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG
Sbjct: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180

Query: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240
           ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD
Sbjct: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240

Query: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300
           VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS
Sbjct: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300

Query: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360
           TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD
Sbjct: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360

Query: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420
           INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF
Sbjct: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420

Query: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480
           LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA
Sbjct: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480

Query: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540
           AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT
Sbjct: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540

Query: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF
Sbjct: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583

BLAST of MC06g0521 vs. ExPASy TrEMBL
Match: A0A6J1C0X0 (Reticulon-like protein OS=Momordica charantia OX=3673 GN=LOC111007258 PE=4 SV=1)

HSP 1 Score: 1105 bits (2858), Expect = 0.0
Identity = 580/583 (99.49%), Postives = 581/583 (99.66%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST
Sbjct: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG
Sbjct: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
           RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG
Sbjct: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180

Query: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240
           ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD
Sbjct: 181 ANLEHLKNPESESNEVEKGIHGSVKEIEKPQVENESSCREFGVCHIPIKSAQNVSIDDSD 240

Query: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300
           VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS
Sbjct: 241 VKDENEGDEGEESEEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIYQKQSPPINKHS 300

Query: 301 TVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360
           TVYQNLSKPTT  +EYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD
Sbjct: 301 TVYQNLSKPTT--NEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKD 360

Query: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420
           INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF
Sbjct: 361 INISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEF 420

Query: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480
           LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA
Sbjct: 421 LLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 480

Query: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540
           AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT
Sbjct: 481 AYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSILT 540

Query: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF
Sbjct: 541 DDWVEEDEVEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 581

BLAST of MC06g0521 vs. ExPASy TrEMBL
Match: A0A6J1JMN2 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 7.21e-288
Identity = 456/595 (76.64%), Postives = 497/595 (83.53%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTRAM KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDS RTI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
             VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 LKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K  P            +KPT T +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPGP----------TSTKPTFTSNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSY KDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYTKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDEVEEVGD-TCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E EE G+ TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEEEEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 563

BLAST of MC06g0521 vs. ExPASy TrEMBL
Match: A0A6J1E2P4 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430032 PE=4 SV=1)

HSP 1 Score: 803 bits (2073), Expect = 1.06e-287
Identity = 457/596 (76.68%), Postives = 497/596 (83.39%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTR M KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRVMPKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDSI TI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSISTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLVVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
           Q VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 QKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K  P            +KPT T +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPGP----------TSTKPTFTSNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSYAKDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYAKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDE--VEEVGDTCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E  VEE   TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEEVEEGEATCQVSIGKGKYGSGTLFGGMSRGKQKKKF 564

BLAST of MC06g0521 vs. ExPASy TrEMBL
Match: A0A6J1JLX7 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485768 PE=4 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 3.80e-283
Identity = 451/595 (75.80%), Postives = 492/595 (82.69%), Query Frame = 0

Query: 1   MDVGRRKAGVGISSVVSGSVWESRMKMDEVKGGIKVFNGEDGSGKDGRAVTRAMSKRIST 60
           MD+GRRK GV  SSVVSGSVWESRMKMDEVKGGIKVFNGEDGS K+GRAVTRAM KR +T
Sbjct: 1   MDLGRRKTGVS-SSVVSGSVWESRMKMDEVKGGIKVFNGEDGSNKEGRAVTRAMLKRTTT 60

Query: 61  ATTDGAAAVSAKRKVWSCDGAEGSEKSPAKGKRNCDEFKSPIQGRKLRSEGNEENSVAIG 120
           AT    + VSAKRKV S DG++GSEKSP +        +SP Q RKLRSE          
Sbjct: 61  ATDT--SVVSAKRKVLSSDGSDGSEKSPIQ--------RSPNQLRKLRSE---------- 120

Query: 121 RIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTIDNDNAKAIIVVAGDGFG 180
            IER+P QIRKSKSEVGSRS+ E +EKT IQLRKVKSDS RTI +DNAKAI+VV G   G
Sbjct: 121 EIERSPNQIRKSKSEVGSRSSVERNEKTPIQLRKVKSDSTRTIVDDNAKAIVVVGGRN-G 180

Query: 181 ANLEHLKNPESESNE----VEKGIHGSVKEIEKPQVENESSCREFGVCHI-----PIKSA 240
            +LEHL+N ESESNE    +EKGIHGSVKEIEKPQVENE+SC+EF VCHI     PIKS 
Sbjct: 181 TDLEHLQNLESESNENLGVIEKGIHGSVKEIEKPQVENETSCKEFEVCHISTIAGPIKST 240

Query: 241 QNVSIDDSDVKDENEGDEGEES--EEEDIEIEKESFDIKEINLPEPKKVVNEVKRFHQIY 300
             VSI+DSDVKDENE +E EE+  EEEDIEIEKESFDIKEINLPEPKKVV+EVKRFHQIY
Sbjct: 241 LKVSINDSDVKDENEANEEEETDEEEEDIEIEKESFDIKEINLPEPKKVVSEVKRFHQIY 300

Query: 301 QKQSPPINKHSTVYQNLSKPTTTPDEYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGS 360
           +K +                    +EYHSFQEKS KLQSLVDLVMWK+VSRSAFVFGIGS
Sbjct: 301 EKPA-------------------SNEYHSFQEKSTKLQSLVDLVMWKEVSRSAFVFGIGS 360

Query: 361 FVIISSSYAKDINISFISVVSYLGLFYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWV 420
           F+IISSSY KDINISFISV+SY+GL YLAATFLYRSIICRGAID E+VRHVVGEEEAIW 
Sbjct: 361 FLIISSSYTKDINISFISVMSYVGLCYLAATFLYRSIICRGAIDMENVRHVVGEEEAIWA 420

Query: 421 LKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480
           LK +LPYLNEFLLKLRALFCGDPATT+KLAVLLFALARCGSSITVWKVAKWGFFGVFTVP
Sbjct: 421 LKLLLPYLNEFLLKLRALFCGDPATTIKLAVLLFALARCGSSITVWKVAKWGFFGVFTVP 480

Query: 481 KVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFV 540
           KVCSSYSSQLAAYAKFWVRRF DAWESCSHKKGVAVAIFVLVWNLSSVVAR+WAAF+LFV
Sbjct: 481 KVCSSYSSQLAAYAKFWVRRFRDAWESCSHKKGVAVAIFVLVWNLSSVVARVWAAFVLFV 540

Query: 541 AFRYYQQSILTDDWVEEDEVEEVGD-TCQASIGKEKHGSGPRFGGMTNAKQKKKF 583
           AFRYYQQS+ T++W EE+E EE G+ TCQ SIGK K+GSG  FGGM+  KQKKKF
Sbjct: 541 AFRYYQQSLQTEEWEEEEEEEEEGEKTCQVSIGKGKYGSGTLFGGMSRGKQKKKF 554

BLAST of MC06g0521 vs. TAIR 10
Match: AT5G58000.1 (Reticulon family protein )

HSP 1 Score: 316.2 bits (809), Expect = 5.4e-86
Identity = 214/503 (42.54%), Postives = 304/503 (60.44%), Query Frame = 0

Query: 105 RKLRSEGNEENSVAIGRIERTPTQIRKSKSEVGSRSNRETHEKTQIQLRKVKSDSIRTID 164
           R L S  + + S ++  +       +K++SE      ++T   T  +++K++S+   T  
Sbjct: 5   RSLSSSDSNDKSPSVSVV------AKKARSESVEGIEKKT---TPGRVKKIRSEVCTT-- 64

Query: 165 NDNAKAIIVVAGDGFGANLEHLKN-PESESNEVEKGIHGSVKEIEKPQVENESSCREFGV 224
                  IV AG+     L  + + P   S + +      V  IE  ++  E   +EFGV
Sbjct: 65  -------IVKAGEFDSVALRKVNSLPSPNSEKSDTKTEQEVTIIENSKIPEE--VKEFGV 124

Query: 225 CH---IPIKSAQNVSIDDSDVKDENEGD-EGEESEEEDIEIEKESFDIKEINLPEPKKVV 284
           C    +  KS +N  ID+ D +  ++ D E +  EEE+ E+EK+S D+KEIN+ +  +V 
Sbjct: 125 CQEMIVSAKSNENEQIDNGDQEIGDQDDYEEDGDEEEEREVEKKSVDVKEINVAKENRVG 184

Query: 285 N-EVKRFHQI----------YQKQSPPINKHST-VYQNLSKPTTTPDEY----HSFQEKS 344
             E+K+F Q            +K SPP+ K +T VY      T++ D +     +F    
Sbjct: 185 GVEIKKFSQFQNRTSPSPSSVRKISPPVIKRATSVYSAPPNSTSSTDRFAEQEDNFTHSQ 244

Query: 345 NKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGLFYLAATFLY 404
           +KLQSLVDLVMW+DVSRS  VFG G+F+IISSSYA D+N SFISVV+Y+GL YL   F+ 
Sbjct: 245 SKLQSLVDLVMWRDVSRSTLVFGFGTFLIISSSYANDLNFSFISVVAYMGLIYLGLMFVL 304

Query: 405 RSIICRGAIDTEHVRHV-VGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPATTMKLAVLL 464
           +S+I RG ++ E  + V V EE+   +L+ ++PYLNE L +LRALF GDP+TT+K+ V+L
Sbjct: 305 KSLIHRGMVEEERHKVVGVREEDVKRMLRLIMPYLNESLHQLRALFSGDPSTTLKMGVVL 364

Query: 465 FALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKG 524
           F LARCGSSIT+W +AK+GF G FT+PK+  SYS+  +AY  FW+RRF DAWESC+HKK 
Sbjct: 365 FVLARCGSSITLWNLAKFGFLGAFTIPKIFISYSTHFSAYGNFWMRRFRDAWESCNHKKA 424

Query: 525 VAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSIL-TDDWVEEDEVEEVGDTCQASIG 581
           VA+A+F LVWNLSSV AR+WAAFML VAFRYYQ  ++ T D  ++DE +   +  +A   
Sbjct: 425 VALALFTLVWNLSSVTARVWAAFMLLVAFRYYQHKMIWTTDQADDDEDDNEEE--EAEEE 484

BLAST of MC06g0521 vs. TAIR 10
Match: AT2G20590.1 (Reticulon family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.9e-35
Identity = 107/302 (35.43%), Postives = 164/302 (54.30%), Query Frame = 0

Query: 256 EDIEIEKESFDIKEI-NLPEPKKVVNEVKRFHQIYQKQSPPINKHSTVYQNLSKPTTTPD 315
           E +E ++ +  I+EI  LP  K+  N   R  +  Q  +PP+   S +      P T   
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTN--GRPKKDKQSSAPPLCSSSDL------PNTCQS 167

Query: 316 EYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGL 375
           + +   E       + DLVMW+DV++S   FG G    +SS +AK +N S  S VS LGL
Sbjct: 168 DLNLIGE------IISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGL 227

Query: 376 FYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPAT 435
             L  +FL  ++  R   DT+   H V E++ +   + VLP  N F+ K   LF G+P+ 
Sbjct: 228 VLLCGSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSM 287

Query: 436 TMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAW 495
           T+K+   L   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++   +    R G+AW
Sbjct: 288 TLKVTPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAW 347

Query: 496 ESCSHKKGVAVAIFVLVWNLSSVVARIWAAFMLFVAFRYYQQSI-LTDDWVEEDEVEEVG 555
             CSHKK +A +     WNL+S+  RI+A F++ V FRY +Q++ LT + VE  E E+  
Sbjct: 348 GVCSHKKILAGSAVTAFWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEEVEPVENEQEE 394

BLAST of MC06g0521 vs. TAIR 10
Match: AT4G28430.1 (Reticulon family protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 100/303 (33.00%), Postives = 163/303 (53.80%), Query Frame = 0

Query: 274 EPKKVVNEVKRFHQIYQKQ--SPPINKHSTVYQNLSKPTTTPDEYHSF--QEKSNKLQSL 333
           EP  VV + K+ +Q  QK+       KHS+V   L+ P+ + D+       +     +++
Sbjct: 137 EPIVVVTDEKKQNQRKQKKLGRSKKEKHSSV-PLLASPSPSSDQPQDVCQGDLERIRENI 196

Query: 334 VDLVMWKDVSRSAFVFGIGSFVIISSSY-AKDINISFISVVSYLGLFYLAATFLYRSIIC 393
            DL+MW+DV++S   FG G    +S+ + AK  N S  S +SYLGL +L  +FL  ++  
Sbjct: 197 SDLIMWRDVAKSTLWFGFGCICFLSTCFAAKGFNFSVFSAISYLGLLFLGVSFLSNTL-- 256

Query: 394 RGAIDTEHVRHV-VGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPATTMKLAVLLFALAR 453
           R  +  E  R + + E++ + + + +LP  N  + K   LF G+PA T+K+A  +   A 
Sbjct: 257 RQRVTEEARRELKLSEDDVLRIARRMLPITNLAISKTSELFSGEPAMTLKVAPFVLMGAE 316

Query: 454 CGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLAAYAKFWVRRFGDAWESCSHKKGVAVAI 513
            G  IT+W++  +GFF  FT+PK+ S Y+SQL    +   RRF +AW  C+HKK VA + 
Sbjct: 317 YGYLITLWRLCAFGFFLSFTIPKLYSCYASQLNQKVECAQRRFVEAWGVCTHKKFVAGSA 376

Query: 514 FVLVWNLSSVVARIWAAFMLFVAFRYYQQSILTDDWVEEDEVEEVGDTCQASIGKEKHGS 571
               WNL+S+  R  A F++ V  RY +Q++  D   EE++ ++     +     E   +
Sbjct: 377 VTAFWNLTSLKTRFIAVFIIVVVIRYRRQNLQLDSEDEEEKKQQEKTHPEQQKSPEDKST 436

BLAST of MC06g0521 vs. TAIR 10
Match: AT2G20590.2 (Reticulon family protein )

HSP 1 Score: 102.4 bits (254), Expect = 1.2e-21
Identity = 77/226 (34.07%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 256 EDIEIEKESFDIKEI-NLPEPKKVVNEVKRFHQIYQKQSPPINKHSTVYQNLSKPTTTPD 315
           E +E ++ +  I+EI  LP  K+  N   R  +  Q  +PP+   S +      P T   
Sbjct: 108 EAVEEKEANLVIEEIVKLPPRKRKTN--GRPKKDKQSSAPPLCSSSDL------PNTCQS 167

Query: 316 EYHSFQEKSNKLQSLVDLVMWKDVSRSAFVFGIGSFVIISSSYAKDINISFISVVSYLGL 375
           + +   E       + DLVMW+DV++S   FG G    +SS +AK +N S  S VS LGL
Sbjct: 168 DLNLIGE------IISDLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGL 227

Query: 376 FYLAATFLYRSIICRGAIDTEHVRHVVGEEEAIWVLKFVLPYLNEFLLKLRALFCGDPAT 435
             L  +FL  ++  R   DT+   H V E++ +   + VLP  N F+ K   LF G+P+ 
Sbjct: 228 VLLCGSFLSNTLCQRKNEDTKRAFH-VSEDDVLRSARRVLPATNFFISKTSELFSGEPSM 287

Query: 436 TMKLAVLLFALARCGSSITVWKVAKWGFFGVFTVPKVCSSYSSQLA 481
           T+K+   L   A  G  IT+W+++ +GFF  FT+PK+ S Y+ Q++
Sbjct: 288 TLKVTPFLLLGAEYGHLITLWRLSAFGFFLSFTIPKLYSCYTHQIS 318