MC06g0485 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0485 vs. ExPASy Swiss-Prot
Match: Q8WV93 (AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 2.9e-69
Identity = 167/417 (40.05%), Postives = 235/417 (56.35%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I A EL D D  Q   ++ LQ+L+++L           Y +  +      L
Sbjct: 74  GPLDHYDFLIKAHELKD-DEHQRRVIQCLQKLHEDLKG---------YNIEAEG-----L 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQ 197
           +S+   +S   P +GLY+YG VGTGKTM+MD+F+  +    RKKR+HFH FML+VH R+ 
Sbjct: 134 FSKLFSRS--KPPRGLYVYGDVGTGKTMVMDMFYAYVEMK-RKKRVHFHGFMLDVHKRIH 193

Query: 198 RHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
           R K               DP+  +A EIS+EA LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 RLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRK--MTSAERGF 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LKE C T ++ S  DYRK  + +A + +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGIDYRKRELPAAGKLY 313

Query: 318 YFVGK-DSSSFLKQKFQQLIGK-STAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKP 377
           Y   + D  + + + F +L  K +      +  V GR L++       A   FEELC++P
Sbjct: 314 YLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEELCERP 373

Query: 378 LGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQEL 437
           LGA+DY  L K F T+ L  IP F L NRT   RF+TL+D  Y+ K R++C+A      L
Sbjct: 374 LGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSL 433

Query: 438 FEKIVTISDAQQIAPRTSTRSRKSDVSD---LCVDNELGFAKDRTISRLTEINSKEY 476
           F      S+ +Q            D ++   +    E  FA  RTISRLTE+ +++Y
Sbjct: 434 FLHQHHDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQY 472

BLAST of MC06g0485 vs. ExPASy Swiss-Prot
Match: Q3V384 (AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 170/417 (40.77%), Postives = 237/417 (56.83%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I + EL + D  Q   ++ LQ+L ++L           Y++ E+ G    L
Sbjct: 74  GPLDHYDFLIKSQELRE-DEHQRRVVQCLQKLQEDLKG---------YSI-EEGGLFSKL 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRL- 197
           +SR        P KGLY+YG VGTGKTM+MD+F+  + +  RKKR+HFH FML+VH R+ 
Sbjct: 134 FSR------NKPPKGLYVYGDVGTGKTMVMDMFYAYVETK-RKKRVHFHGFMLDVHRRIH 193

Query: 198 -------QRHKGVA----DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
                  +R  G      DP+  +A EIS E  LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 HLKQSLPKRKAGFMAKSYDPIAPIAEEISQETSLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAERG--F 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LKE C T ++ S  DYRK   A  G  +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCDTLQLDSGVDYRKRELAPAGKLY 313

Query: 318 YFVGK-DSSSFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 377
           Y   + D  + + + F +L  K      P+ ++ V GR L++       A   FEELC++
Sbjct: 314 YLTSEADVEAVVDKLFDELAQKQNDLTSPRILK-VQGRELRLNKACGSVADCTFEELCER 373

Query: 378 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 437
           PLGA+DY  L K F T+ +  IP F L  RT A RF+TL+D  Y+ K R++C+A      
Sbjct: 374 PLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFITLIDNFYDFKVRIICSASAPISS 433

Query: 438 LF--EKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEY 476
           LF  +   + SD  +I       S+ S    +    E  FA  RTISRLTE+ +++Y
Sbjct: 434 LFLHQHQDSESDQSRILMDDLGLSQDSAGLSMFTGEEEIFAFQRTISRLTEMQTEQY 471

BLAST of MC06g0485 vs. ExPASy Swiss-Prot
Match: Q32PX9 (AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.2e-68
Identity = 166/417 (39.81%), Postives = 235/417 (56.35%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I A EL   D  Q   ++ LQ+L ++L           Y + E+ G    L
Sbjct: 74  GPLAHYDFLIKAQEL-KNDEHQRRVVQCLQKLQEDLKG---------YNI-EEGGLFSKL 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQ 197
           +SR        P KGLY+YG VGTGKTM+MD+F++ +     K+R+HFH FML+VH R+ 
Sbjct: 134 FSR------NKPPKGLYVYGDVGTGKTMVMDMFYEYVEVK-SKRRVHFHGFMLDVHKRIH 193

Query: 198 RHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
           R K               DP+  +A EIS EA LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 RLKQSLPKRKVGFMAKSYDPIAPIAEEISQEASLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRK--MTSAERGF 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LKE C T ++ S  DYRK  +T A + +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGVDYRKRELTPAGKLY 313

Query: 318 YFVGK-DSSSFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 377
           Y   + D  + + + F +L  K      P+ ++ V GR L++       A   FEELC++
Sbjct: 314 YLTSEADVGTVMDKLFDELAQKQNDLTSPRILK-VQGRELRLNKACGTVADCTFEELCER 373

Query: 378 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 437
           PLGA+DY  L K F T+ +  IP F L  RT   RF+TL+D  Y+ K R++C+A      
Sbjct: 374 PLGASDYLELSKNFDTVIIRNIPQFSLAKRTQVRRFITLIDNFYDFKVRIICSASVPISS 433

Query: 438 LF--EKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEY 476
           LF  +   + SD  ++       S+ S    +    E  F+  RT+SRLTE+ +++Y
Sbjct: 434 LFVYQHQDSESDQSRVLMDDLGLSQDSAGLSMFTGEEEIFSFQRTLSRLTEMQTEQY 471

BLAST of MC06g0485 vs. ExPASy Swiss-Prot
Match: P46441 (Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.6e-59
Identity = 150/438 (34.25%), Postives = 229/438 (52.28%), Query Frame = 0

Query: 75  RVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSEKSGRS 134
           R   P+  YE+RI + EL+  D  Q  T +EL+ LY+ L N           V  ++   
Sbjct: 33  RFLTPMQAYEQRIESKELLP-DKVQKKTTQELEDLYNTLKN------YQPKPVRVETSSG 92

Query: 135 RWLWSRFIPQSSYSP---------VKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHF 194
              + RF+ +   +P          KG+Y+YG VG GKT LMD+F+       +K+R+HF
Sbjct: 93  GGFFGRFMKKEQSAPKIELLNTTAPKGMYIYGSVGGGKTTLMDMFYSCCDDIPKKQRVHF 152

Query: 195 HDFMLNVHS---RLQRHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDV 254
           + FM  VH    ++++ +G              DP   VA  I++E+ L+C DEF VTD+
Sbjct: 153 NSFMSKVHGLIHKVKQERGPQDRAFNSEKPLPFDPTLPVAEMIANESWLICFDEFQVTDI 212

Query: 255 ADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSA 314
           ADA+IL  LF HLF+ G++ +ATSNR P+ LY+ GLQR  F+PFI  L  RC    + S 
Sbjct: 213 ADAMILKSLFTHLFNEGIVCIATSNRHPNDLYKNGLQRSNFIPFIGVLLNRCNVAAMDSG 272

Query: 315 TDYRKMTSAERGFYFV--GKDSSSFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGA 374
            DYR++  +    YFV    D+ S +++ F+ L  +      P+ +    GR L      
Sbjct: 273 VDYRRIAQSGDTNYFVTTQTDAKSQMERMFKILCSQENDIIRPRTI-THFGRDLTFQRTC 332

Query: 375 NGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYEN 434
                  FEELC++PLG +DY  + + FHT+ +  +P   L  ++   RF+TL+D +Y+N
Sbjct: 333 GQVLDSNFEELCNRPLGGSDYIQIGQFFHTVLIHDVPQLTLLLKSQMRRFITLIDTLYDN 392

Query: 435 KARLLCTAEGSPQELF---EKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRT 482
           + R++ +AE    +LF   +K   ++D Q++             + +    E  FA DRT
Sbjct: 393 RVRVVISAEVPLDQLFSFTDKPKDLADEQRMLMDDLKLGDTDTSASVFTGEEEMFAFDRT 452

BLAST of MC06g0485 vs. ExPASy Swiss-Prot
Match: Q5TYS0 (Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.6e-54
Identity = 135/375 (36.00%), Postives = 199/375 (53.07%), Query Frame = 0

Query: 120 CRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWR 179
           CR D    +EK  +           S   P +G Y+YG VGTGKTMLMDLF+     N R
Sbjct: 129 CRPDENVSNEKEDQQE-------ESSKPHPPQGYYIYGNVGTGKTMLMDLFY-SFVENRR 188

Query: 180 KKRIHFHDFMLNVHSRLQRHK-----------GVADPLEVVAGEISDEAILLCLDEFMVT 239
           KKR+HF+ FML+VH R+ + K            + DP+  VA EI++E  L+C DEF V 
Sbjct: 189 KKRVHFNGFMLDVHRRIHKLKQSLPKRRIGKMTMYDPIFPVAMEIAEETCLICFDEFQVV 248

Query: 240 DVADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIG 299
           D+ADA+IL +LF  LF  GV++VATSNR P++LY+ GLQR  F+PFI  LKE C    + 
Sbjct: 249 DIADAMILKQLFEGLFKCGVVVVATSNRPPEELYKNGLQRAAFVPFIGVLKEYCRIVSLD 308

Query: 300 SATDY--RKMTSAERGFYFVGK-DSSSFLKQKFQQL-IGKSTAGPQEVEVVMGRILQVPS 359
           +  D+  R+M  A R +Y   + D+ + +   F++L   ++      V  V GR + +  
Sbjct: 309 TGIDFRTREMKPAGRLYYISSEPDAENAVNALFEELAFRQNDVTRPRVLNVQGREVTLSR 368

Query: 360 GANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMY 419
                A   F+ELC++PLGA DY  + + F T+ +  +P   L  +  A RF TL+D  Y
Sbjct: 369 TCGTIADCSFQELCEQPLGAGDYLEIARCFDTVIIRNVPYLQLGMKDQARRFTTLIDNFY 428

Query: 420 ENKARLLCTAEGSPQELFEKIVTIS----DAQQIAPRTSTRSRKSDVSDLCVDNELGFAK 476
           + K R++  A+     L ++         D   +     T      ++    D E+ FA 
Sbjct: 429 DQKVRVVMLADAPLDRLLDQGQMTGEEARDRLMLDELGLTDEASKRITLFTADEEI-FAF 488

BLAST of MC06g0485 vs. NCBI nr
Match: XP_022135330.1 (AFG1-like ATPase [Momordica charantia] >XP_022135331.1 AFG1-like ATPase [Momordica charantia] >XP_022135332.1 AFG1-like ATPase [Momordica charantia])

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR
Sbjct: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
           ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC
Sbjct: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQA 500
           QLVAEKQNWQGHHNQDALQA
Sbjct: 481 QLVAEKQNWQGHHNQDALQA 500

BLAST of MC06g0485 vs. NCBI nr
Match: XP_038880754.1 (AFG1-like ATPase isoform X4 [Benincasa hispida])

HSP 1 Score: 928 bits (2398), Expect = 0.0
Identity = 464/499 (92.99%), Postives = 481/499 (96.39%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY RHLRSAFSQ+TR+HT VL RGQRQRQL+HTN GS FIVK DRYRTP+ +  R
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
            LSVDAAKLSNGDY+VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSA+AC
Sbjct: 61  TLSVDAAKLSNGDYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCV+HEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSSSFLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRK+D+SDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQ 499
           +LVAEK+NW+   NQDALQ
Sbjct: 481 ELVAEKRNWEDGDNQDALQ 499

BLAST of MC06g0485 vs. NCBI nr
Match: XP_038880753.1 (AFG1-like ATPase isoform X3 [Benincasa hispida])

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 464/500 (92.80%), Postives = 481/500 (96.20%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY RHLRSAFSQ+TR+HT VL RGQRQRQL+HTN GS FIVK DRYRTP+ +  R
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
            LSVDAAKLSNGDY+VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSA+AC
Sbjct: 61  TLSVDAAKLSNGDYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCV+HEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSSSFLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTIS-RLTEINSKEYLEQH 480
           LFEKIVTISDAQQIAPRTSTRSRK+D+SDLCVDNELGFAKDRTIS RLTEINSKEYLEQH
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISSRLTEINSKEYLEQH 480

Query: 481 AQLVAEKQNWQGHHNQDALQ 499
           A+LVAEK+NW+   NQDALQ
Sbjct: 481 AELVAEKRNWEDGDNQDALQ 500

BLAST of MC06g0485 vs. NCBI nr
Match: XP_038880752.1 (AFG1-like ATPase isoform X2 [Benincasa hispida])

HSP 1 Score: 923 bits (2385), Expect = 0.0
Identity = 464/501 (92.61%), Postives = 481/501 (96.01%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY RHLRSAFSQ+TR+HT VL RGQRQRQL+HTN GS FIVK DRYRTP+ +  R
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNG--DYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAE 120
            LSVDAAKLSNG  DY+VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSA+
Sbjct: 61  TLSVDAAKLSNGVADYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQ 120

Query: 121 ACRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180
           ACRLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW
Sbjct: 121 ACRLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180

Query: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240
           RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR
Sbjct: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240

Query: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTS 300
           LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCV+HEIGSATDYRK+TS
Sbjct: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTS 300

Query: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELC 360
           AERGFYFVGKDSSSFLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELC
Sbjct: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELC 360

Query: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420
           DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP
Sbjct: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420

Query: 421 QELFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQ 480
            ELFEKIVTISDAQQIAPRTSTRSRK+D+SDLCVDNELGFAKDRTISRLTEINSKEYLEQ
Sbjct: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQ 480

Query: 481 HAQLVAEKQNWQGHHNQDALQ 499
           HA+LVAEK+NW+   NQDALQ
Sbjct: 481 HAELVAEKRNWEDGDNQDALQ 501

BLAST of MC06g0485 vs. NCBI nr
Match: XP_038880751.1 (AFG1-like ATPase isoform X1 [Benincasa hispida])

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 464/502 (92.43%), Postives = 481/502 (95.82%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY RHLRSAFSQ+TR+HT VL RGQRQRQL+HTN GS FIVK DRYRTP+ +  R
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNG--DYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAE 120
            LSVDAAKLSNG  DY+VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSA+
Sbjct: 61  TLSVDAAKLSNGVADYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQ 120

Query: 121 ACRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180
           ACRLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW
Sbjct: 121 ACRLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180

Query: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240
           RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR
Sbjct: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240

Query: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTS 300
           LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCV+HEIGSATDYRK+TS
Sbjct: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTS 300

Query: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELC 360
           AERGFYFVGKDSSSFLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELC
Sbjct: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELC 360

Query: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420
           DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP
Sbjct: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420

Query: 421 QELFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTIS-RLTEINSKEYLE 480
            ELFEKIVTISDAQQIAPRTSTRSRK+D+SDLCVDNELGFAKDRTIS RLTEINSKEYLE
Sbjct: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISSRLTEINSKEYLE 480

Query: 481 QHAQLVAEKQNWQGHHNQDALQ 499
           QHA+LVAEK+NW+   NQDALQ
Sbjct: 481 QHAELVAEKRNWEDGDNQDALQ 502

BLAST of MC06g0485 vs. ExPASy TrEMBL
Match: A0A6J1C0D0 (AFG1-like ATPase OS=Momordica charantia OX=3673 GN=LOC111007319 PE=3 SV=1)

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR
Sbjct: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
           ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC
Sbjct: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQA 500
           QLVAEKQNWQGHHNQDALQA
Sbjct: 481 QLVAEKQNWQGHHNQDALQA 500

BLAST of MC06g0485 vs. ExPASy TrEMBL
Match: A0A5A7SYX6 (Lactation elevated protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00320 PE=3 SV=1)

HSP 1 Score: 915 bits (2364), Expect = 0.0
Identity = 461/499 (92.38%), Postives = 474/499 (94.99%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY RHLRSAFSQ+TR+HT VL R QRQRQL+HTN  S FIVK DRYRTP  +  R
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRLQRQRQLIHTNSESLFIVKPDRYRTPRCINLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
            LSVDAAKLSNGDY VAGPLVEYERRIAAGELVDGD+CQVGTLRELQRLYDEL+NSAEAC
Sbjct: 61  TLSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDNCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS FLKQKFQQLIGKS  G QEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSIFLKQKFQQLIGKSIPGRQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEG+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPYE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRK+DVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQ 499
           +LVAEK+NWQ   +QDA Q
Sbjct: 481 ELVAEKRNWQNGDDQDASQ 499

BLAST of MC06g0485 vs. ExPASy TrEMBL
Match: A0A6J1E1Y3 (AFG1-like ATPase OS=Cucurbita moschata OX=3662 GN=LOC111430095 PE=3 SV=1)

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 459/500 (91.80%), Postives = 477/500 (95.40%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRYVR+LRSAFSQ+T H T VL RGQRQ+QLVHTN  S FIVKSD++R+P+ L  R
Sbjct: 1   MRGVNRYVRNLRSAFSQKTHHQTDVLRRGQRQKQLVHTNSESFFIVKSDKFRSPKYLNLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
           ALSVDAAKL NGDY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSAEAC
Sbjct: 61  ALSVDAAKLCNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVG  +S+FLKQKFQQLIGKS A P EVEVVMGRILQVPSGANGCAYFPFEELC+K
Sbjct: 301 RGFYFVGNGASTFLKQKFQQLIGKSIARPHEVEVVMGRILQVPSGANGCAYFPFEELCNK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRT+AYRFVTLVDVMYENKARLLCTAEGSP+E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTSAYRFVTLVDVMYENKARLLCTAEGSPRE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEK+VTISDAQQIAPRTS RS+K+DVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKVVTISDAQQIAPRTSARSQKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQA 500
           QLVAEK+ WQ   NQDALQ+
Sbjct: 481 QLVAEKRKWQEDDNQDALQS 500

BLAST of MC06g0485 vs. ExPASy TrEMBL
Match: A0A6J1JJY1 (AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111485772 PE=3 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 457/500 (91.40%), Postives = 476/500 (95.20%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRYVRHLRSAFSQ+T + T VL RGQRQ+QLVHTN  S FIVKSD++R+P+ L  R
Sbjct: 1   MRGVNRYVRHLRSAFSQKTHNQTDVLRRGQRQKQLVHTNSESFFIVKSDKFRSPKYLNLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
           ALSVDAAKL NGDY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSAEAC
Sbjct: 61  ALSVDAAKLCNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPD LYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVG  +S+FLKQKFQQLIGKSTA P EVEVVMGR+LQVPSGANGCAYFPFEELC+K
Sbjct: 301 RGFYFVGNGASTFLKQKFQQLIGKSTARPHEVEVVMGRVLQVPSGANGCAYFPFEELCNK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRT+AYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTSAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEK+VTISDAQQIAPRTS RS+K+D+SDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKVVTISDAQQIAPRTSARSQKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQA 500
           QLVAEK+ WQ   NQDALQ+
Sbjct: 481 QLVAEKRKWQEDDNQDALQS 500

BLAST of MC06g0485 vs. ExPASy TrEMBL
Match: A0A1S4DV19 (lactation elevated protein 1 OS=Cucumis melo OX=3656 GN=LOC103487322 PE=3 SV=1)

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 458/499 (91.78%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60
           MR VNRY  HLRSA SQ+TR+HT VL R QRQRQL+HTN  S FIVK DRYRTP  +  R
Sbjct: 1   MRGVNRYACHLRSAVSQKTRNHTDVLRRLQRQRQLIHTNSESLFIVKPDRYRTPRCINLR 60

Query: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120
            LSVDAAKLSNGDY VAGPLVEYERRIAAGELVDGD+CQVGTLRELQRLYDEL+NSAEAC
Sbjct: 61  TLSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDNCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS FLKQKFQQLIGKS  G QEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSIFLKQKFQQLIGKSIPGRQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420
           PLGAADYFGLFKKFHTLALEG+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPYE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRK+DVS+LCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSNLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAEKQNWQGHHNQDALQ 499
           +LVAEK+NWQ   +QDA Q
Sbjct: 481 ELVAEKRNWQNGDDQDASQ 499

BLAST of MC06g0485 vs. TAIR 10
Match: AT4G28070.2 (AFG1-like ATPase family protein )

HSP 1 Score: 701.4 bits (1809), Expect = 5.1e-202
Identity = 341/418 (81.58%), Postives = 375/418 (89.71%), Query Frame = 0

Query: 70  SNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSE 129
           SNGD ++AGPLVEYERRI AGEL+DGD CQ+GTLRELQRLYDEL+ SA+ACRLDRY+ S 
Sbjct: 53  SNGDGKIAGPLVEYERRIVAGELLDGDLCQLGTLRELQRLYDELVQSADACRLDRYSASA 112

Query: 130 KSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFM 189
           KS RS W W++F+  SS SPVKGLYLYGGVGTGKTMLMDLFF QLP++WR +RIHFH+FM
Sbjct: 113 KSTRSNWFWNKFVSHSSVSPVKGLYLYGGVGTGKTMLMDLFFHQLPASWRTQRIHFHNFM 172

Query: 190 LNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVI 249
           L+VHSRLQ+HKG+ DPLEVV  EI+DEAILLCLDEFMV DVADALILNRLF HLF+NG+I
Sbjct: 173 LSVHSRLQKHKGLEDPLEVVGLEIADEAILLCLDEFMVNDVADALILNRLFRHLFNNGII 232

Query: 250 LVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAERGFYFVGKD 309
           LVATSNRAPD LYEGGLQRDLFLPFISTLKERCV  EIGS+ DYRK+TSAE GFYF+GKD
Sbjct: 233 LVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIGKD 292

Query: 310 SSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFG 369
            S  LKQKFQ L+G   AGPQ VEVVMGR LQVP  A+GCAYF FEELCD+PLGAADY G
Sbjct: 293 ISGLLKQKFQLLVGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLG 352

Query: 370 LFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQELFEKIVTIS 429
           LFKKFHTLALEG+P FGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP EL E+IVTIS
Sbjct: 353 LFKKFHTLALEGVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVTIS 412

Query: 430 DAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHAQLVAEKQ 488
           DAQQIAPRTS+RSRKSD  DLCVDNELGFAKDRTISRLTE+NSKEYLEQH++++ EKQ
Sbjct: 413 DAQQIAPRTSSRSRKSDDPDLCVDNELGFAKDRTISRLTEMNSKEYLEQHSRMLQEKQ 470

BLAST of MC06g0485 vs. TAIR 10
Match: AT4G30490.1 (AFG1-like ATPase family protein )

HSP 1 Score: 690.6 bits (1781), Expect = 9.0e-199
Identity = 339/457 (74.18%), Postives = 386/457 (84.46%), Query Frame = 0

Query: 25  VLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISRALSVDAAKLSNGDYRVAGPLVEYE 84
           ++G  Q Q  L      S+   +S  +R        A      +++  D    GPLVEYE
Sbjct: 36  LIGVNQNQALLNTNTDNSSLYSRSSIFRGLSAEAVEAADPATTRVTVSDVNRTGPLVEYE 95

Query: 85  RRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSEKSGRSRWLWSRFIPQ 144
           RRI+ GEL+ GD CQ+  LRELQRLYDEL++S + CRLDRY  S+KS RSRW WSR +PQ
Sbjct: 96  RRISNGELMTGDICQISALRELQRLYDELVDSVDTCRLDRYNTSDKSSRSRWFWSRLMPQ 155

Query: 145 SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQRHKGVAD 204
           +SYSPVKGLYLYGGVGTGKTMLMDLFFDQLP  W+K+RIHFHDFML+VHSRLQ+HKG++D
Sbjct: 156 TSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWKKQRIHFHDFMLSVHSRLQKHKGLSD 215

Query: 205 PLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVILVATSNRAPDKLYEG 264
           PLEVVA EI+ +AILLCLDEFMVTDVADALILNRLFGHLFSNGVILVATSNR PDKLYEG
Sbjct: 216 PLEVVAQEIAHDAILLCLDEFMVTDVADALILNRLFGHLFSNGVILVATSNRNPDKLYEG 275

Query: 265 GLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAERGFYFVGKDSSSFLKQKFQQLIGK 324
           GLQRDLFLPFIS+LKER V HEIGSA DYRK+TSAE+GFYF+GKD S+ LKQKF+QLIG 
Sbjct: 276 GLQRDLFLPFISSLKERSVVHEIGSAVDYRKLTSAEQGFYFIGKDLSTLLKQKFRQLIGD 335

Query: 325 S-TAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIP 384
           +  A PQ VEVVMGR LQ+P GANGCAYFPFEELCD+PLGAADYFGLFKKFHTLAL+ IP
Sbjct: 336 NVVARPQVVEVVMGRKLQIPLGANGCAYFPFEELCDRPLGAADYFGLFKKFHTLALDEIP 395

Query: 385 IFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQELFEKIVTISDAQQIAPRTSTRSR 444
           +FGLHNRTAAYRFVTLVDVMYEN+ARLLCTAE +PQEL EKI+TIS+A+ + PRTS+RSR
Sbjct: 396 VFGLHNRTAAYRFVTLVDVMYENRARLLCTAEANPQELLEKIITISEAKSMGPRTSSRSR 455

Query: 445 KSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 481
           K+DV++LCVDNELGFAKDRTISRLTE+NSKEYLE HA
Sbjct: 456 KNDVTELCVDNELGFAKDRTISRLTEMNSKEYLEHHA 492

BLAST of MC06g0485 vs. TAIR 10
Match: AT4G28070.1 (AFG1-like ATPase family protein )

HSP 1 Score: 673.3 bits (1736), Expect = 1.5e-193
Identity = 331/418 (79.19%), Postives = 365/418 (87.32%), Query Frame = 0

Query: 70  SNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEACRLDRYAVSE 129
           SNGD ++AGPLVEYERRI AGEL+DGD CQ+GTLRELQRLYDEL+ SA+ACRLDRY+ S 
Sbjct: 53  SNGDGKIAGPLVEYERRIVAGELLDGDLCQLGTLRELQRLYDELVQSADACRLDRYSASA 112

Query: 130 KSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFM 189
           KS RS W W++F+  SS SPVKGLYLYGGVGTGKTMLMDLFF QLP++WR +RIHFH+FM
Sbjct: 113 KSTRSNWFWNKFVSHSSVSPVKGLYLYGGVGTGKTMLMDLFFHQLPASWRTQRIHFHNFM 172

Query: 190 LNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVI 249
           L+VHSRLQ+HKG+ DPLEVV  EI+DEAILLCLDEFMV DVADALILNRLF HLF+NG+I
Sbjct: 173 LSVHSRLQKHKGLEDPLEVVGLEIADEAILLCLDEFMVNDVADALILNRLFRHLFNNGII 232

Query: 250 LVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAERGFYFVGKD 309
           LVATSNRAPD LYEGGLQRDLFLPFISTLKERCV  EIGS+ DYRK+TSAE GFYF+GKD
Sbjct: 233 LVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIGKD 292

Query: 310 SSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFG 369
            S  LKQKFQ L+G   AGPQ VEVVMGR LQVP  A+GCAYF FEELCD+PLGAADY G
Sbjct: 293 ISGLLKQKFQLLVGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLG 352

Query: 370 LFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQELFEKIVTIS 429
           LF          +P FGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP EL E+IVTIS
Sbjct: 353 LF---------SVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVTIS 412

Query: 430 DAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHAQLVAEKQ 488
           DAQQIAPRTS+RSRKSD  DLCVDNELGFAKDRTISRLTE+NSKEYLEQH++++ EKQ
Sbjct: 413 DAQQIAPRTSSRSRKSDDPDLCVDNELGFAKDRTISRLTEMNSKEYLEQHSRMLQEKQ 461

BLAST of MC06g0485 vs. TAIR 10
Match: AT2G25530.1 (AFG1-like ATPase family protein )

HSP 1 Score: 181.0 bits (458), Expect = 2.3e-45
Identity = 131/404 (32.43%), Postives = 194/404 (48.02%), Query Frame = 0

Query: 151 KGLYLYGGVGTGKTMLMDLFFDQLPSNWR-KKRIHFHDFMLNVHSRLQRH---KGVADPL 210
           KGLY+YG VG GKTMLMD+F+       R ++R HFH+ ML ++ ++ ++    G   P+
Sbjct: 242 KGLYIYGNVGCGKTMLMDMFYGATDGIIRHRQRFHFHEAMLKINEQMHKYWKENGAEKPM 301

Query: 211 ------------------EVVAGE---------------ISDE-----------AILLCL 270
                             E +AGE               ++D+           A +LC 
Sbjct: 302 QYSISSWIMNLPVDEKVKEWLAGEEFYKQQLQMKHILPAVADKFLVDQQSSKKGASILCF 361

Query: 271 DEFMVTDVADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERC 330
           DE    DV   + L+ +   L + G +LVATSNRAP +L + G+Q+++F  FIS L++ C
Sbjct: 362 DEIQTVDVFAIVALSGIMSRLLTTGTVLVATSNRAPRELNQDGMQKEIFDKFISKLEKHC 421

Query: 331 VTHEIGSATDYRKM---TSAERGFYF------VGKDSSSFLKQKFQQLIGKSTAGPQEVE 390
               IGS  DYR++    SAE   Y       V ++     +Q   Q  G+ T+    + 
Sbjct: 422 EIISIGSEVDYRRVAAKNSAENVHYLWPLNDAVLEEFEKMWRQVTDQYGGEITSA--TLP 481

Query: 391 VVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAA 450
           V+ GR ++VP   +G A F FE LC +P+GAADY  + K +HT+ +  IP   +  R  A
Sbjct: 482 VMFGRTVEVPESCSGVARFTFEYLCGRPVGAADYIAVAKNYHTIFISDIPAMSMEIRDKA 541

Query: 451 YRFVTLVDVMYENKARLLCTAEGSPQELFEKIV--TISDAQQIAPRTSTR---------- 478
            RF+TLVD +Y +   L+ +AE +  ELF+     T+ D +     T T           
Sbjct: 542 RRFITLVDELYNHHCCLVSSAETAIDELFQGTAEGTLFDLESFQFETETEDSRLRRDVLA 601