MC06g0466 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0466
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tRNA (Adenine(37)-N6)-methyltransferase
Location	MC06: 3822550 .. 3826651 (-)
RNA-Seq Expression	MC06g0466
Synteny	MC06g0466

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACAATGATATTTTGTTAATTTTCTTTTTTAATTTGTCCCTCTTGTTCAATACAAGGTAAATGTTGTTTGGAAATTTAATACTGCCAAAAATTAAAAAAGAAAAAGAGTTTTTTGTTCTACTTCTAAATTCCAATAATCACAAACTCGGAAATTGCTTACAACTCACGGTCGCGGGTCGCGTTGCATAAGCTGCCGGTCGAGTTCGTTTCCCCGCTGAAGCTGCTTTCCAACCCTGAAGACTAAGAAATGGCCTACGGCGCAGAATCAAGATGGTCGACAGCCGCCATGAGCATCATACTGGCGTTTATCTCCGCCTCCGCCATCGTATCTCGTAAAACTTCGAAAACTTTTAAGATCCTCGAGCTCCTCTCTCTCAGTTCTCTGTTGTCCTACATTTTTCTCGCAATTGAAGTTTTCTACTCTTTTTCCCAGTTTATCTCGTGACCAGGAAGTCGAGAGAGTTGGGTTCCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCGGCGTCTTTAAAGAAAACCACCGCCGAGAGACAAGGCCGAATACGGGCTCAGCAGGTATTTGCTCCTCTGCAGCATCGCTTCATTTTTCAATCTTATTAACTTATTATTGATTCTTTTACTTTTTCCCCGAATTCGTAGGCTTTAAGGAAAGCACTTGCGGACCCAAAATCTGATAATGTGGAGTGTACTTCATATCCCATGACACCAATTGGTGTTATCCAGTCAGCCTTCTCTACTAGGTTAGTTTGAAATTTCATTATTTGGCAGTCTAAACATCAATTGGGTTGATCTTTTCTCATTGTTGTATCCATCCATTAAAGTACATTTATGGATATGTTTAAGCCATAGTCTTCACTCAGATGTAGAAATCGAGCAATCAACAACTTTAATCGGGTATTTTTTAGGAATGGAACTCCAAGGCAATCTTTAGTCGTACCTCTTGCTAGGGCACGATTGGTGTTTTCTGCTCGTATTCCTCCAGCTTCAATTGAAGGTCTCGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAACACCGATCTAGATAAGTTGTGGAAGAATCCATCTAAATCAAGATTCAAGGCAAAAGTAAGTTTCTTCTTTTATTGATGCTCCTTAAAAATGGTCTTCATATTTCTATTATTCTTATGACTTCCTAAAATCCCATAAAAGGACAGCTCAATATTAGGTTAGAGTACCAAGGTTGAAAGGCAAAAGGGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCGATTGGACTCACTATTGCAAAGGTAAGATAAAAGTTTCAAGCTCAACTGCCATTTATAAATCTTGTGAGTTGGAAAGCTGTAATGTGATAGTCCTGTGGGGGACCTCCTCGGCCCCTGCCTTTAGGCTGTTTTCCTCTTGTTGGTCATGATATATTTTGTTTCTTATAAAAATAATAATAATAATAAATAAATCTTGTGAGTTGATTCAGAAAGCCGCATCGGATCATAGACATTTGGGCCCACCCCACCAATTCTAGATAGTTCTCATTGTCTTACGTTTGGTCTAATACGTTTTGTATCATCAATGTAATTTAGTAATAGGAACTCATGGTTACAAAGTAATTCATGACAGCCAAATGCAAAAGAAAAGAAAAATGGAGGAGTTTTGAATTTTAGATTAAGAAATATGCTATATTACCAATTCAGGTGGAGGCTATCCAGGGGCATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTGTAAGTTTTTTTGTTTCTGATTTCTTCCATCCAGCAGGATAAAGATTTAGTCTTGAAAGAAACCATTATTTCTTGGTGGTGATGATTGTTGTGATTTCTTGCTTTGTTTGAATTTTGTTGCAGCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGATAGTGTACAAGATGCAACAGTCCCAGAGTGGCTAATGGTATTTCTTCAATATTTCTATGGCTAAATGTTATTTTAGAATTTATTTGATGCTTATGATTTCCTATTCATCCTTTAAATTAATTTTTAGCCATGCATTTGTCCATTTATCTGTCCAACGGCCAACGTAGATTTATGTCCAGTCATCAGTGTTGCTCTGCTGCTTTTCAAACTTCAAAATGTGGAAAACCTCTACATATATGCAGGCCCAGATAACTGGTTGCATGCAAATAACTTGAGAAATATTAGCTTTTCATATACTTTCAGGTGCTATTTTTCATAGGTGGAGGTATCGATACTCCATGACCATGTAATATGTTTTATGCATCCTTCATGTCAATTTGACATCTTATTTTATGCAACCGCAGGGGGACAATTTGTTGAGTGTTGCATCAATTTGCTTCTCTGAAGAATTTACTTCCACTCTATCTGATTGTTGGCCACTGATGGTGAGGTTTTTTAATTTGTGCTGGCTAGTTCATTTTAATTACTGATTTGAAGTTCAGCTTGTTCATCTTCATGCAATACGCCAAAAAATTATATATTTTTTCGCGCACGGGAAAAAGAAACAATATATATATACATATTTTTGATAGGAAACAGAAACTTGTCATTAGAAAGAACCGAAGAACTGCCTAAAGGTACAGGGATGAGAAAGCCCCGCCTTACAAAAACCTAATGAAAAACAACTTTTCAATTGCGACAAAGCATCGAAAGATTGTAATTACAAAAAAAATCCCTGTAGTTTGGACACCAAGTTGAGGCGGTAATTTGTACACAATTTAAAAAATAATTAGGAGAATGTATCTTCTTGTTGAAAAACAGTAGGGAATATTTTACCTATCCATTCTAAGTTTTCCTTAAGTTTTGGATATTGGAGAGGTCTAATGTAAGATCAACAGTCTGGTTTCTTTTGTTTTTTTGGTATCAATCAAAGGGGCCTTTTAAACTATTATATTTTATGCCCCAAGGGATGCCCTTGCTGGTTAGGGCTTGGAGTTTTGGGTGATAGTAAGCCTTAGGTTGGAGCCAGTGAAAACTCTTTTTGTGTCCCGGTGGTGCTGTTGCACGCAAGGTCTAAATGCCTCATGATAATTTGTCTCTAGAATTTTACATGAACATTTATCTGGTTAGTTTCTTATTCTTTTACTTGTAATTTACTTCAAAAAATAAAAAGAGAGAGAGAACTGCTTTGTTGTCTGATTATGATTTTTTTTTTTTTTGAACTCCACATTACAAAGAGGTCGTGCTCTCAACTCTTGTATTGTACAAAGTAGGATATTCTGGGTCAGTATGTCTTTTGATGTTTCTAGCTCCCATATTTGCAACTAAATTTCACATGCATCCATATTTTTCTTGTTTCAGGAAAAGAAATCGCATTATGCATCTGCAGACGAGTTTAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCACTATCCCAACGAACTCGACCTCATGAATCCCACATTGAGACAACAACAGGACATGAATTGTCCGATGGAAGTTCAGATTTAGATACTCACCTATATGACAAAGCTTCTGGCGGTACCCAAATTTCTTCTAAGAACATCATCTATCACCTACTTGTAGATGGATTAAATGTGTCATACACGATAGGTTGCCACGGTGACGTTGTTGTGGTTAAAGCAGAACTCGCATCACCCAGCTCAAGAACAAGAAGTAATTAACGCAATGCAAGCTCTTTTCAGTAAATCGAGGTAACTTGTCTTCCTGGCCTTGGATTAATTCTAAAACAATTCCCAATGTGGGGGCTTTTCTTTACTTTAAATTATACTACCTTGTATGGAGCTAGATCTCTTTACATTTCTTACTCATTCTTGAGAGTGGAATGCTGGATATGGATATGGTCTTAATTATGAGTTGTTGTTAGGTGGTTGTTGAAATAGTTTTGGCTGAAAATGAGTGGCTAGTTTTGATTTTGATGAGGGATCGTTTGACTTTTGTGTAACAATCTTGAAAGAAGAAAAGGAAGTTATATTTTAGATCTTTAGCTCCAAAAGTAAGGCTGTTTATACAAGTGACCAGGCCCCTATCAGGCTAACGTGATGTACTTGTGATTTAGGGAATTTGAAAACAGGATCCCTCTGTTGAAGATGTGATCCAAACGAAATGTTCAC

mRNA sequence

TAACAATGATATTTTGTTAATTTTCTTTTTTAATTTGTCCCTCTTGTTCAATACAAGGTAAATGTTGTTTGGAAATTTAATACTGCCAAAAATTAAAAAAGAAAAAGAGTTTTTTGTTCTACTTCTAAATTCCAATAATCACAAACTCGGAAATTGCTTACAACTCACGGTCGCGGGTCGCGTTGCATAAGCTGCCGGTCGAGTTCGTTTCCCCGCTGAAGCTGCTTTCCAACCCTGAAGACTAAGAAATGGCCTACGGCGCAGAATCAAGATGGTCGACAGCCGCCATGAGCATCATACTGGCGTTTATCTCCGCCTCCGCCATCGTATCTCTTTATCTCGTGACCAGGAAGTCGAGAGAGTTGGGTTCCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCGGCGTCTTTAAAGAAAACCACCGCCGAGAGACAAGGCCGAATACGGGCTCAGCAGGCTTTAAGGAAAGCACTTGCGGACCCAAAATCTGATAATGTGGAGTGTACTTCATATCCCATGACACCAATTGGTGTTATCCAGTCAGCCTTCTCTACTAGGAATGGAACTCCAAGGCAATCTTTAGTCGTACCTCTTGCTAGGGCACGATTGGTGTTTTCTGCTCGTATTCCTCCAGCTTCAATTGAAGGTCTCGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAACACCGATCTAGATAAGTTGTGGAAGAATCCATCTAAATCAAGATTCAAGGCAAAAGTTAGAGTACCAAGGTTGAAAGGCAAAAGGGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCGATTGGACTCACTATTGCAAAGGTGGAGGCTATCCAGGGGCATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGATAGTGTACAAGATGCAACAGTCCCAGAGTGGCTAATGGGGGACAATTTGTTGAGTGTTGCATCAATTTGCTTCTCTGAAGAATTTACTTCCACTCTATCTGATTGTTGGCCACTGATGGAAAAGAAATCGCATTATGCATCTGCAGACGAGTTTAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCACTATCCCAACGAACTCGACCTCATGAATCCCACATTGAGACAACAACAGGACATGAATTGTCCGATGGAAGTTCAGATTTAGATACTCACCTATATGACAAAGCTTCTGGCGGTACCCAAATTTCTTCTAAGAACATCATCTATCACCTACTTGTAGATGGATTAAATGTGTCATACACGATAGGTTGCCACGGTGACGTTGTTGTGGTTAAAGCAGAACTCGCATCACCCAGCTCAAGAACAAGAAGTAATTAACGCAATGCAAGCTCTTTTCAGTAAATCGAGGTAACTTGTCTTCCTGGCCTTGGATTAATTCTAAAACAATTCCCAATGTGGGGGCTTTTCTTTACTTTAAATTATACTACCTTGTATGGAGCTAGATCTCTTTACATTTCTTACTCATTCTTGAGAGTGGAATGCTGGATATGGATATGGTCTTAATTATGAGTTGTTGTTAGGTGGTTGTTGAAATAGTTTTGGCTGAAAATGAGTGGCTAGTTTTGATTTTGATGAGGGATCGTTTGACTTTTGTGTAACAATCTTGAAAGAAGAAAAGGAAGTTATATTTTAGATCTTTAGCTCCAAAAGTAAGGCTGTTTATACAAGTGACCAGGCCCCTATCAGGCTAACGTGATGTACTTGTGATTTAGGGAATTTGAAAACAGGATCCCTCTGTTGAAGATGTGATCCAAACGAAATGTTCAC

Coding sequence (CDS)

ATGGCCTACGGCGCAGAATCAAGATGGTCGACAGCCGCCATGAGCATCATACTGGCGTTTATCTCCGCCTCCGCCATCGTATCTCTTTATCTCGTGACCAGGAAGTCGAGAGAGTTGGGTTCCAAGGTTCGAGAGCTCGAGGCTTCTCTGGCGGCGTCTTTAAAGAAAACCACCGCCGAGAGACAAGGCCGAATACGGGCTCAGCAGGCTTTAAGGAAAGCACTTGCGGACCCAAAATCTGATAATGTGGAGTGTACTTCATATCCCATGACACCAATTGGTGTTATCCAGTCAGCCTTCTCTACTAGGAATGGAACTCCAAGGCAATCTTTAGTCGTACCTCTTGCTAGGGCACGATTGGTGTTTTCTGCTCGTATTCCTCCAGCTTCAATTGAAGGTCTCGGAGAATATTCCCATTGCTGGATTATATATGTCTTTCATTTAAACACCGATCTAGATAAGTTGTGGAAGAATCCATCTAAATCAAGATTCAAGGCAAAAGTTAGAGTACCAAGGTTGAAAGGCAAAAGGGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCGATTGGACTCACTATTGCAAAGGTGGAGGCTATCCAGGGGCATCAAATTCTACTCTCTGGTGCTGATCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGATAGTGTACAAGATGCAACAGTCCCAGAGTGGCTAATGGGGGACAATTTGTTGAGTGTTGCATCAATTTGCTTCTCTGAAGAATTTACTTCCACTCTATCTGATTGTTGGCCACTGATGGAAAAGAAATCGCATTATGCATCTGCAGACGAGTTTAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCACTATCCCAACGAACTCGACCTCATGAATCCCACATTGAGACAACAACAGGACATGAATTGTCCGATGGAAGTTCAGATTTAGATACTCACCTATATGACAAAGCTTCTGGCGGTACCCAAATTTCTTCTAAGAACATCATCTATCACCTACTTGTAGATGGATTAAATGTGTCATACACGATAGGTTGCCACGGTGACGTTGTTGTGGTTAAAGCAGAACTCGCATCACCCAGCTCAAGAACAAGAAGTAATTAA

Protein sequence

MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAERQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPEWLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQRTRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCHGDVVVVKAELASPSSRTRSN

Homology

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BLAST of MC06g0466 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 9.8e-33
Identity = 73/151 (48.34%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 92  PIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 151
           P+G ++S FS +NGTPRQ  +   +RA L    RI   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGVFATRSPHRPCPIGLTIAKVEAIQGHQIL 211
           N  L            KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E ++G  I 
Sbjct: 92  NGHLS----------CKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSVQDATVP 240
           LSG D++ GTPVLD+KPY+   DS Q+   P
Sbjct: 152 LSGIDMIHGTPVLDIKPYIAEYDSPQNVMEP 172

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BLAST of MC06g0466 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.8e-32
Identity = 72/150 (48.00%), Postives = 97/150 (64.67%), Query Frame = 0


Query: 92  PIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 151
           PIG ++S F  + GTPRQ  +   +RA L     I   P  S+ GL E+SH WI++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 152 NTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGVFATRSPHRPCPIGLTIAKVEAIQGHQIL 211
           N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E ++G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSVQDATV 239
           LSG D++DGTPVLD+KPY+   DS Q+ +V
Sbjct: 152 LSGVDMIDGTPVLDIKPYIADYDSPQNLSV 171

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BLAST of MC06g0466 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.1e-31
Identity = 70/147 (47.62%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 92  PIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 151
           PIG ++S FS + GTPRQ  +   +RA L     I   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGVFATRSPHRPCPIGLTIAKVEAIQGHQIL 211
           N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E ++G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSVQD 236
           LSG D++ GTPVLD+KPY+   DS Q+
Sbjct: 152 LSGIDMIHGTPVLDIKPYIADYDSPQN 168

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BLAST of MC06g0466 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.1e-27
Identity = 65/155 (41.94%), Postives = 96/155 (61.94%), Query Frame = 0


Query: 87  SYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARIPP----ASIEGLGEYSHCWI 146
           ++ ++PIG I+S F  +   PRQ L+ P AR  L     +PP     ++EGL + SH W+
Sbjct: 2   THSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTLEL---LPPFDQVEALEGLEQVSHVWL 61

Query: 147 IYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKR-VGVFATRSPHRPCPIGLTIAKVEA 206
           +++FH             + + + KVR PRL G R +GVFATR+ HRP  IG ++ ++E 
Sbjct: 62  LFLFH----------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEG 121

Query: 207 IQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDA 237
            +  ++ LSG DL+DGTPVLD+KPY+PY D+V DA
Sbjct: 122 FEAGRLWLSGIDLLDGTPVLDIKPYVPYADAVADA 143

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BLAST of MC06g0466 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 9.8e-25
Identity = 62/155 (40.00%), Postives = 94/155 (60.65%), Query Frame = 0


Query: 87  SYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARLVFSARIPP----ASIEGLGEYSHCWI 146
           S+ ++PIG ++S F  +   PRQ  + P A   L     +PP     ++EGL + SH W+
Sbjct: 2   SHSISPIGHVRSCFKEKFAIPRQPQLAPAATGVLEL---LPPFDTGDAVEGLEQVSHVWL 61

Query: 147 IYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKR-VGVFATRSPHRPCPIGLTIAKVEA 206
           I++FH             + + + KVR PRL G + +GVFATR+ HRP  +G ++ K+E 
Sbjct: 62  IFLFH----------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEK 121

Query: 207 IQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDA 237
           ++  ++ LSG DL+DGTPV+D+KPY+PY D V DA
Sbjct: 122 VEPGRLWLSGIDLLDGTPVIDIKPYVPYADIVPDA 143

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BLAST of MC06g0466 vs. NCBI nr
Match: XP_022134721.1 (uncharacterized protein LOC111006924 [Momordica charantia])

HSP 1 Score: 749 bits (1935), Expect = 7.90e-273
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVVVKAELASPSSRTRSN
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

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BLAST of MC06g0466 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 659 bits (1700), Expect = 5.11e-237
Identity = 325/380 (85.53%), Postives = 359/380 (94.47%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY AESRWS AA+S+ LAFISASA++SLY+VT+KSRELG+KVRELEASLA+SLKKT AE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+VECTSYPMTPIG I+S+FSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GDNLL+VASICFSEEF  TLS+CWPLMEKKS YAS DEF+NFVK+VLSWDIRS+SQR
Sbjct: 241 WLLGDNLLTVASICFSEEFIPTLSECWPLMEKKSLYASEDEFRNFVKEVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
            +PH+SH++ TTGHE  DGSSD + HL DK S G+Q SS+NIIYHLLVDGL+VSYTIGCH
Sbjct: 301 NQPHDSHVKPTTGHECFDGSSDSEGHLDDKVSSGSQSSSENIIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           G+VVV K ELASPSSRTRSN
Sbjct: 361 GNVVVGKVELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. NCBI nr
Match: TYK09463.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 639 bits (1648), Expect = 4.26e-229
Identity = 320/380 (84.21%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY AES WSTAAMS+ L FISASAI+SLYLVT+KSRELG+KV++LEASLAASLKKT AE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+VECTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGL EYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GDN+L+VASICFSEEFTS LS CWPL+EKKS YAS +E KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T TG EL D SSD D H+ DK S  +Q SSK+ IYHLLVDGL+VSYTIG H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELASPSSRTRSN
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 637 bits (1642), Expect = 2.90e-228
Identity = 320/380 (84.21%), Postives = 351/380 (92.37%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           M Y A+SRWSTAAMS+ LAFISASAI+SLYLVT+KSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+V+CTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQ+ATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GDN+L+VASICFSEEFTSTLS CWPL EKKS YAS DE KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T      SD SSD D HL DK S  +Q SSK+ IYHLLVDGL+VSYTIGCH
Sbjct: 301 SRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELAS SSRTRSN
Sbjct: 361 GDVVVDKVELASSSSRTRSN 375

[Close]

BLAST of MC06g0466 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 635 bits (1637), Expect = 2.02e-227
Identity = 318/380 (83.68%), Postives = 351/380 (92.37%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY AES WSTAAMS+ L FISASAI+SLYLVT+KSRELG+KV++LEASLAASLKKT AE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+VECTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGL EYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GD++L+VASICFSEEFTS LS CWPL+EKKS YAS +E KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T TG EL D SSD D H+ DK S  +Q  SK+ IYHLLVDGL+VSYTIG H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELASPSSRTRSN
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 3.83e-273
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVVVKAELASPSSRTRSN
Sbjct: 361 GDVVVVKAELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 639 bits (1648), Expect = 2.06e-229
Identity = 320/380 (84.21%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY AES WSTAAMS+ L FISASAI+SLYLVT+KSRELG+KV++LEASLAASLKKT AE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+VECTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGL EYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GDN+L+VASICFSEEFTS LS CWPL+EKKS YAS +E KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T TG EL D SSD D H+ DK S  +Q SSK+ IYHLLVDGL+VSYTIG H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELASPSSRTRSN
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 1.41e-228
Identity = 320/380 (84.21%), Postives = 351/380 (92.37%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           M Y A+SRWSTAAMS+ LAFISASAI+SLYLVT+KSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+V+CTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQ+ATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GDN+L+VASICFSEEFTSTLS CWPL EKKS YAS DE KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T      SD SSD D HL DK S  +Q SSK+ IYHLLVDGL+VSYTIGCH
Sbjct: 301 SRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGCH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELAS SSRTRSN
Sbjct: 361 GDVVVDKVELASSSSRTRSN 375

[Close]

BLAST of MC06g0466 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 635 bits (1637), Expect = 9.77e-228
Identity = 318/380 (83.68%), Postives = 351/380 (92.37%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY AES WSTAAMS+ L FISASAI+SLYLVT+KSRELG+KV++LEASLAASLKKT AE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+A+PKSD+VECTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFSARIPPASIEGL EYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDSVQDATVP+
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL+GD++L+VASICFSEEFTS LS CWPL+EKKS YAS +E KNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCH 360
           +RPH SHI+T TG EL D SSD D H+ DK S  +Q  SK+ IYHLLVDGL+VSYTIG H
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIGYH 360

Query: 361 GDVVVVKAELASPSSRTRSN 380
           GDVVV K ELASPSSRTRSN
Sbjct: 361 GDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of MC06g0466 vs. ExPASy TrEMBL
Match: A0A6J1JCZ9 (uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678 PE=4 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 1.49e-223
Identity = 312/375 (83.20%), Postives = 347/375 (92.53%), Query Frame = 0


Query: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60
           MAY   SR STAAMSI LAF+SASAI+SLY VT+KSRELGSKVRELEASLAASLKKT AE
Sbjct: 1   MAYAVVSRSSTAAMSITLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+++PKSD+VECT YPM PIG IQS+FSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180
           VFS+RIPPASIEGLGEYSHCW+IYVFHLNT+LDKLWK+PSKS+FKAKV+VPRLKG++VG+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240
           FATRSPHRPCPIG+TIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDS+QDATVP+
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300
           WL G+N+LSVASICFSEEFT +LS+CWPL+EKKS YASADE K+FVKQVLSWDIRSLSQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLSQR 300

Query: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGT-QISSKNIIYHLLVDGLNVSYTIGC 360
           ++PHES++ TT GHELSDGSSD D HL +    GT Q SSK+++YHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 HGDVVVVKAELASPS 374
            GDVVV K E+ASPS
Sbjct: 361 QGDVVVRKVEVASPS 375

[Close]

BLAST of MC06g0466 vs. TAIR 10
Match: AT4G28020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0066 (InterPro:IPR001378); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 396.0 bits (1016), Expect = 3.5e-110
Identity = 200/362 (55.25%), Postives = 265/362 (73.20%), Query Frame = 0


Query: 10  STAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAERQGRIRAQQ 69
           +TAA++  LA +S SA  SLY   R+  +L ++++E E SL ++L+K+ +ER+GR++AQQ
Sbjct: 11  TTAALAFTLAVVSVSAAFSLYRWRRREEDLENRIKEFEKSLGSTLEKSASERKGRVKAQQ 70

Query: 70  ALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARLVFS-ARIPP 129
           ALR+ALA  +S ++  T YPM PIG IQS FSTRNGTPRQ L+V LARA L+F  A +PP
Sbjct: 71  ALREALAQTESHDLHSTIYPMRPIGTIQSCFSTRNGTPRQPLLVSLARACLIFDPALVPP 130

Query: 130 ASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGVFATRSPHR 189
           AS+EGL EYSHCWI+YVFHLNTD++KLW+ PS+S+ KAKVRVPRL G+R GVFATRSPHR
Sbjct: 131 ASLEGLEEYSHCWILYVFHLNTDIEKLWRKPSQSKLKAKVRVPRLNGERKGVFATRSPHR 190

Query: 190 PCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPEWLMGDNLL 249
           PCPIGLT+AKVE IQ  ++LLSG DLVDGTPVLD+KPYLPY DS+Q A+VP W+  D  L
Sbjct: 191 PCPIGLTVAKVEEIQKDKVLLSGVDLVDGTPVLDIKPYLPYSDSIQGASVPNWVKEDCSL 250

Query: 250 SVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQRTRPHESHI 309
           +VAS+ FS+ F+S+++ CW L+EKKS Y+SADEF++ + QVLSWDIRS+SQR +P ++  
Sbjct: 251 AVASVTFSDTFSSSITSCWKLIEKKSLYSSADEFRSLITQVLSWDIRSMSQRNKPQDT-- 310

Query: 310 ETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIGCHGDVVVVKA 369
                         LD               + I+YHL+++GL+VSY I    +++V   
Sbjct: 311 --------------LD---------------EEIVYHLVLEGLDVSYMIDNESNILVQDV 341

Query: 370 EL 371
            L
Sbjct: 371 SL 341

The following BLAST results are available for this feature:

BLAST of MC06g0466 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9BU70	9.8e-33	48.34	tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=...	[more]
Q562D6	2.8e-32	48.00	tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV...	[more]
Q4V7E0	4.1e-31	47.62	tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE...	[more]
Q9RPT0	6.1e-27	41.94	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]
O68638	9.8e-25	40.00	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]

BLAST of MC06g0466 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134721.1	7.90e-273	100.00	uncharacterized protein LOC111006924 [Momordica charantia]	[more]
XP_038879626.1	5.11e-237	85.53	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida]	[more]
TYK09463.1	4.26e-229	84.21	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa]	[more]
XP_004149885.1	2.90e-228	84.21	uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ...	[more]
XP_008453657.1	2.02e-227	83.68	PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo]	[more]

BLAST of MC06g0466 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYL5	3.83e-273	100.00	uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A5D3CC84	2.06e-229	84.21	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LTM6	1.41e-228	84.21	TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P...	[more]
A0A1S3BW92	9.77e-228	83.68	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1JCZ9	1.49e-223	83.20	uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678...	[more]

BLAST of MC06g0466 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28020.1	3.5e-110	55.25	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023370	TrmO-like, N-terminal domain	PFAM	PF01980	TrmO	coord: 105..231 e-value: 1.5E-42 score: 144.6
IPR023370	TrmO-like, N-terminal domain	TIGRFAM	TIGR00104	TIGR00104	coord: 88..236 e-value: 4.2E-29 score: 99.5
IPR023370	TrmO-like, N-terminal domain	PROSITE	PS51668	TSAA_2	coord: 90..235 score: 67.757072
IPR023370	TrmO-like, N-terminal domain	CDD	cd09281	UPF0066	coord: 92..226 e-value: 4.03876E-49 score: 159.965
IPR036414	YaeB, N-terminal domain superfamily	GENE3D	2.40.30.70		coord: 89..243 e-value: 3.2E-53 score: 181.4
None	No IPR available	PANTHER	PTHR12818:SF0	TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE	coord: 12..368
IPR040372	YaeB-like	PANTHER	PTHR12818	UNCHARACTERIZED	coord: 12..368
IPR036413	YaeB-like superfamily	SUPERFAMILY	118196	YaeB-like	coord: 86..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0466.1	MC06g0466.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016430	tRNA (adenine-N6-)-methyltransferase activity