MC06g0465 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0465 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.0e-189
Identity = 311/463 (67.17%), Postives = 373/463 (80.56%), Query Frame = 0

Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYS 318
             D  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS YS
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 319 CTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRN 378
           CTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 379 PWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 438
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 439 GGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           GG  K A LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of MC06g0465 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 5.8e-184
Identity = 308/477 (64.57%), Postives = 376/477 (78.83%), Query Frame = 0

Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLA 309
           +SLK   +E++L++  D   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PYLA
Sbjct: 256 YSLKP-AEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLA 315

Query: 310 QGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRS 369
           QGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +VRS
Sbjct: 316 QGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRS 375

Query: 370 IVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISA 429
           IVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISA
Sbjct: 376 IVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISA 435

Query: 430 KHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           KHIAQAAA+R E+RGG  + A LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 KHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of MC06g0465 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.6e-181
Identity = 294/454 (64.76%), Postives = 369/454 (81.28%), Query Frame = 0

Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPDDKGDLP 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++  + +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQNLEIEGEIP 274

Query: 265 TKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTS 324
                YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF T 
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 325 CSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEY 384
           C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K +Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 385 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKACL 444
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A +
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

Query: 445 QMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 QMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of MC06g0465 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 8.6e-71
Identity = 154/424 (36.32%), Postives = 223/424 (52.59%), Query Frame = 0

Query: 57  DPTAKITTPENVLPPEA-----------PLVVFINPRSGGRHGQKLKQRLQHLISDEQVF 116
           +P      P+ +L P++           P+VVFIN RSGG+ G  L +  + L++  QVF
Sbjct: 17  EPLTDYCIPDYILNPDSEQVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVF 76

Query: 117 DLLDVKPHEFVQYGLRC-LEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHK 176
           DL +  P E V + L C  E L   GD  A Q +  LR++VAGGDGT  W+LG +++L K
Sbjct: 77  DLSEEAP-EKVLHRLYCNFEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-K 136

Query: 177 QDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSL 236
               P  P+  +PLGTGN+L  SFGWG   P   + AVK  L +    +  ++DSWH+ +
Sbjct: 137 LSHPP--PIATVPLGTGNNLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIM 196

Query: 237 SMPAGEK--------VEPPHSL---KQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYFS 296
            M A ++        +E PHSL    +V+    LN               Y G F+NYFS
Sbjct: 197 RMRAPQEGPCEPIAPLELPHSLHAFHRVSGSDSLN---------MEGYHTYRGGFWNYFS 256

Query: 297 IGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILR 356
           +GMDAQV+Y FH  R + P   +  +TN+  Y+     QGWF  S +  P+ R +  + +
Sbjct: 257 MGMDAQVSYEFHSERKRNPEKFKNQLTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQLAK 316

Query: 357 IHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGL 416
           + I K     WE++ +P S+RSIV LNL +++ G NPWG P    ++ R       DDGL
Sbjct: 317 VRIMKRPGGQWEELKIPRSIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGL 376

Query: 417 LEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDS 458
           +E+ G +  WH   ++        +AQA  IR E   G  +   +++DGEPWKQ + KD 
Sbjct: 377 IEVVGFRDAWHGLVLLAPNGHGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDD 426

BLAST of MC06g0465 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.1e-70
Identity = 149/410 (36.34%), Postives = 228/410 (55.61%), Query Frame = 0

Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNK 296
            ++ PHSL ++         P D+ ++      Y G F+NYFS+GMDAQV+Y FH  R  
Sbjct: 213 TLKLPHSLPRIF--------PSDQENM-EGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKL 272

Query: 297 RPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVP 356
            P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + +P
Sbjct: 273 HPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLHIP 332

Query: 357 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 416
            S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   ++ 
Sbjct: 333 QSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLS 392

Query: 417 DIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQI-SKDSSTFVEI 460
                  +AQA  +R+E + G  K A +++DGEPWKQ + S D +  VEI
Sbjct: 393 PNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of MC06g0465 vs. NCBI nr
Match: XP_022134720.1 (diacylglycerol kinase 7-like [Momordica charantia])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPP--DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPP  DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

BLAST of MC06g0465 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840 bits (2170), Expect = 2.14e-305
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           QAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of MC06g0465 vs. NCBI nr
Match: XP_023516208.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839 bits (2167), Expect = 6.61e-305
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

BLAST of MC06g0465 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 838 bits (2166), Expect = 8.70e-305
Identity = 410/473 (86.68%), Postives = 438/473 (92.60%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT   +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  --PENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           QAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of MC06g0465 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 837 bits (2161), Expect = 5.03e-304
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           QAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of MC06g0465 vs. ExPASy TrEMBL
Match: A0A6J1BZ41 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111006923 PE=3 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 472/474 (99.58%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPP--DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPP  DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

BLAST of MC06g0465 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 837 bits (2161), Expect = 2.43e-304
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           QAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of MC06g0465 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 835 bits (2158), Expect = 7.52e-304
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

BLAST of MC06g0465 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 828 bits (2139), Expect = 5.46e-301
Identity = 405/473 (85.62%), Postives = 436/473 (92.18%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA Y GVFYNYFSIGMDAQVAYGFHHLRNKRP LA+GP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           QAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

BLAST of MC06g0465 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 827 bits (2136), Expect = 1.69e-300
Identity = 405/475 (85.26%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLPP--EAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++PP  EAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA Y GVFYNYFSIGMDAQVAYGFHHLRNKRP LA+G
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 472
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

BLAST of MC06g0465 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 663.3 bits (1710), Expect = 1.5e-190
Identity = 311/463 (67.17%), Postives = 373/463 (80.56%), Query Frame = 0

Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYS 318
             D  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS YS
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 319 CTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRN 378
           CTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 379 PWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 438
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 439 GGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           GG  K A LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of MC06g0465 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 645.2 bits (1663), Expect = 4.1e-185
Identity = 308/477 (64.57%), Postives = 376/477 (78.83%), Query Frame = 0

Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLA 309
           +SLK   +E++L++  D   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PYLA
Sbjct: 256 YSLKP-AEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLA 315

Query: 310 QGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRS 369
           QGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +VRS
Sbjct: 316 QGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRS 375

Query: 370 IVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISA 429
           IVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISA
Sbjct: 376 IVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISA 435

Query: 430 KHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           KHIAQAAA+R E+RGG  + A LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 KHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of MC06g0465 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 637.1 bits (1642), Expect = 1.1e-182
Identity = 294/454 (64.76%), Postives = 369/454 (81.28%), Query Frame = 0

Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPDDKGDLP 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++  + +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQNLEIEGEIP 274

Query: 265 TKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTS 324
                YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF T 
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 325 CSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEY 384
           C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K +Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 385 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKACL 444
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A +
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

Query: 445 QMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 QMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of MC06g0465 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 268.9 bits (686), Expect = 8.0e-72
Identity = 149/410 (36.34%), Postives = 228/410 (55.61%), Query Frame = 0

Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNK 296
            ++ PHSL ++         P D+ ++      Y G F+NYFS+GMDAQV+Y FH  R  
Sbjct: 213 TLKLPHSLPRIF--------PSDQENM-EGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKL 272

Query: 297 RPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVP 356
            P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + +P
Sbjct: 273 HPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLHIP 332

Query: 357 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 416
            S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   ++ 
Sbjct: 333 QSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLS 392

Query: 417 DIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQI-SKDSSTFVEI 460
                  +AQA  +R+E + G  K A +++DGEPWKQ + S D +  VEI
Sbjct: 393 PNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of MC06g0465 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 264.6 bits (675), Expect = 1.5e-70
Identity = 149/414 (35.99%), Postives = 218/414 (52.66%), Query Frame = 0

Query: 71  PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLG 130
           P +P++VFIN +SGG+ G +L    + L++  QVFDL    P + ++     LE L D  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 131 DACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGW 190
           D  A+Q R+KL+++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAFGW 156

Query: 191 GGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK---------VEPPHSLKQV 250
           G   P   + AV+  L +    +V  +D+WH+ + M   ++         +E PHSL   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 251 TQESDLNEPPDDKGDLPTKLAC--YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPV 310
            + S  +E          K  C  + G F+NYFS+GMDAQ++Y FH  R   P   +  +
Sbjct: 217 HRVSPTDE--------LNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQL 276

Query: 311 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 370
            N+  Y    CTQGWF  S    P  R +  + ++ I   N   W+ + +P S+RSIV L
Sbjct: 277 VNQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCL 336

Query: 371 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 430
           NL +++ G NPWG P P     RG      DDGL+E+ G +  WH   ++        +A
Sbjct: 337 NLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLA 396

Query: 431 QAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSST-FVEIKRVPFQSLMINGD 473
           QA  IR E   G      ++MDGEPWKQ +  D  T  VEI  +   +++   D
Sbjct: 397 QANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435