MC06g0377 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0377 vs. ExPASy Swiss-Prot
Match: P21885 (Arginine decarboxylase OS=Bacillus subtilis (strain 168) OX=224308 GN=speA PE=1 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 1.4e-90
Identity = 183/483 (37.89%), Postives = 277/483 (57.35%), Query Frame = 0

Query: 38  PLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGP 97
           PL   LK  A +   +FH PGH +G      F Q IG      DL  +  LD+L  P+G 
Sbjct: 7   PLYTGLKKHASRQPVQFHIPGHKKGAGMDPEFRQFIGENALSIDLINIEPLDDLHAPKGI 66

Query: 98  ILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSG 157
           I +AQ  AA+ FGA  T+F V GT+  I T +MA C PG+ IIIPRN H S+++A+V SG
Sbjct: 67  IKQAQDLAAEAFGADHTFFSVQGTSGAIMTMVMAVCGPGDKIIIPRNVHKSIMTAIVFSG 126

Query: 158 AIPKYIMPEYDSNWDIAGGVTPSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEIS 217
           A+P +I PE D+   I+ G+T   A  A+     E      + V +PTY G+ ++L  I 
Sbjct: 127 AVPIFIHPEIDNELGISHGITLESAKRAL----TEHPDAKGLLVINPTYFGVAADLKSIV 186

Query: 218 QICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGN 277
           ++ HS+ +P++VDEAHG H  F  ++P SA+Q G D+ A S HK+  SLTQSS+L+M   
Sbjct: 187 ELAHSFDVPVLVDEAHGVHIHFHDELPLSAMQAGADIAATSVHKLGGSLTQSSILNMREG 246

Query: 278 IVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKIS 337
           +V ++RV   L  L +TS SYLLLASLD AR +L+    ++  + + LANQ ++++N+I 
Sbjct: 247 LVSKDRVQSILSMLTTTSTSYLLLASLDVARKRLATEGRQLAEETLKLANQTRDRLNQIE 306

Query: 338 GISILEVPIFSNSPA--IDPLRLTIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFV 397
           GI  +   I  +  A   DP +L I  + LGL+G++ ++ L ++ +I  EL    +I  +
Sbjct: 307 GIYCVGSEILGSKAAYSYDPTKLIISVKSLGLTGHDVEKWLRESFNIEVELSDLYNILCI 366

Query: 398 INLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIV-LNPRDAFFAKKR 457
              G  ++D +RLV   E ++ +A  +  +  +   T    P+I ++ + PRDAF+A   
Sbjct: 367 FTPGDSQNDADRLV---EALTEIAQQMSEQDVTHQQTEVLLPEIPLLAMTPRDAFYANTE 426

Query: 458 RENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSS 517
              +KE  G++  E +  YPPGIP+ IPGE+I+EE + Y+      G  + G  D  L  
Sbjct: 427 VIPLKEASGRIIAEFVMVYPPGIPIFIPGEIITEENISYIFKNLDAGLPVQGPEDSTLHM 482

BLAST of MC06g0377 vs. ExPASy Swiss-Prot
Match: Q9K9K5 (Arginine decarboxylase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=speA PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.7e-86
Identity = 187/491 (38.09%), Postives = 275/491 (56.01%), Query Frame = 0

Query: 31  ISKESCPPLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLPELPELDN 90
           +S++   PL + +   A+ N  +FH PGH +G     +F   IG      DL  +  LD+
Sbjct: 1   MSRQEQTPLFSGVVAHAKHNPVQFHIPGHKKGAGMDPAFRSFIGDNALSIDLINIGPLDD 60

Query: 91  LFCPEGPILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPRNSHVSVI 150
           L  P G I EAQ+ AA+ FGA  T+F V GT+  I T IM+   PGE II+PRN H S++
Sbjct: 61  LHHPHGIIKEAQELAAEAFGADHTFFSVQGTSGAIMTMIMSVVGPGEKIIVPRNVHKSIM 120

Query: 151 SALVLSGAIPKYIMPEYDSNWDIAGGVTPSQASGAIKDSEMEGH-KVSAVFVTSPTYHGI 210
           SA+V SGA P +I PE D    I+ G+T      A     ++ H     + V +PTY GI
Sbjct: 121 SAIVFSGATPVFIHPEIDPLLGISHGITIEAVEKA-----LDAHPDAKGLLVINPTYFGI 180

Query: 211 CSNLSEISQICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVLSSLTQS 270
            +NL +I ++CHS  +P++VDEAHG H  F   +P SA+Q G D+ A S HK+  SLTQS
Sbjct: 181 AANLKKIVELCHSRDVPVLVDEAHGVHIHFHEALPLSAMQAGADMAATSVHKLGGSLTQS 240

Query: 271 SMLHMSGNIVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQA 330
           S+L++   +V  +RV   +  L +TS SYLLLASLDAAR  L+ N   +    I LA+QA
Sbjct: 241 SILNVREGLVSAKRVQTIISMLTTTSTSYLLLASLDAARKHLATNGRDLIGYTIQLADQA 300

Query: 331 KNKINKISGISILEVPIFSNSPA--IDPLRLTIGFQQLGLSGYEADEILHKNHDIVCELV 390
           +++IN I G+  +   I         DP +L I  + LG++GY+A+  L +++ I  EL 
Sbjct: 301 RDQINAIDGLYCVGKEILGTIATYDYDPTKLIISVKNLGITGYDAEVWLREHYQIEVELS 360

Query: 391 GTQSITFVINLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAP-FPDIKIVLNPR 450
              +I  +++ G  E +++ LV  + +++ L      E RS +S   P  P   + ++PR
Sbjct: 361 DLYNILCIVSFGDTEREMDLLVKALSELADLHKHGICE-RSPVSVYVPNIP--TLAMSPR 420

Query: 451 DAFFAKKRRENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGASISG 510
           DAF+A+      ++ VG+   E I  YPPGIP+ IPGE+I+E  L Y+      G  + G
Sbjct: 421 DAFYAETEVVPFEDSVGRTIAEFIMVYPPGIPILIPGEIITESNLAYIRENNRAGLPVQG 480

Query: 511 ASDPQLSSLLV 518
             D    +L V
Sbjct: 481 PEDDTFRTLRV 483

BLAST of MC06g0377 vs. ExPASy Swiss-Prot
Match: Q81MS2 (Arginine decarboxylase OS=Bacillus anthracis OX=1392 GN=speA PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.7e-83
Identity = 167/483 (34.58%), Postives = 269/483 (55.69%), Query Frame = 0

Query: 38  PLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGP 97
           PL  AL   +++N  +FH PGH +G+     F + IG      DL  +  LD+L  P+G 
Sbjct: 10  PLFTALVEHSKRNPIQFHIPGHKKGQGMDPEFREFIGHNALAIDLINIAPLDDLHHPKGM 69

Query: 98  ILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSG 157
           I EAQ  AA  FGA  T+F + GT+  I T +M+ C PG+ I++PRN H SV+SA++ SG
Sbjct: 70  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 129

Query: 158 AIPKYIMPEYDSNWDIAGGVTPSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEIS 217
           A P ++ PE D    I+ G+T      A++    E      + V +PTY G  ++L +I 
Sbjct: 130 AKPIFMHPEIDPKLGISHGITIQSVKKALE----EHSDAKGLLVINPTYFGFAADLEQIV 189

Query: 218 QICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGN 277
           Q+ HSY IP++VDEAHG H  F  ++P SA+Q G D+ A S HK+  SLTQSS+L++   
Sbjct: 190 QLAHSYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEG 249

Query: 278 IVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKIS 337
           +V+ + V   +  L +TS SY+LLASLD AR +L+     +  + I LA Q +N IN I 
Sbjct: 250 LVNVKHVQSIISMLTTTSTSYILLASLDVARKRLATEGKALIEQTIQLAEQVRNAINDIE 309

Query: 338 GISILEVPIFSNSPAI--DPLRLTIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFV 397
            +      +         DP ++ +  + LG++G++A+  L + ++I  EL    +I  +
Sbjct: 310 HLYCPGKEMLGTDATFNYDPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNILCL 369

Query: 398 INLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIV-LNPRDAFFAKKR 457
           +  G  E +   L++ ++D   L++I + +    +      P+I ++ L+PRDAF+++  
Sbjct: 370 VTFGDTESETNTLIAALQD---LSAIFKNKADKGVRIQVEIPEIPVLALSPRDAFYSETE 429

Query: 458 RENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSS 517
               +   G++  + +  YPPGIP+  PGE+I+++ L+Y+      G  + G  D  L +
Sbjct: 430 VIPFENAAGRIIADFVMVYPPGIPIFTPGEIITQDNLEYIRKNLEAGLPVQGPEDMTLQT 485

BLAST of MC06g0377 vs. ExPASy Swiss-Prot
Match: Q819L4 (Arginine decarboxylase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=speA PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.7e-78
Identity = 159/449 (35.41%), Postives = 250/449 (55.68%), Query Frame = 0

Query: 38  PLVNALKISAEQNAARFHFPGHNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGP 97
           PL  AL   +++N  +FH PGH +G+    +F + IG      DL  +  LD+L  P+G 
Sbjct: 7   PLFTALVEHSKRNPIQFHIPGHKKGQGMDPTFREFIGHNALAIDLINIAPLDDLHHPKGM 66

Query: 98  ILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSG 157
           I EAQ  AA  FGA  T+F + GT+  I T +M+ C PG+ I++PRN H SV+SA++ SG
Sbjct: 67  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 126

Query: 158 AIPKYIMPEYDSNWDIAGGVTPSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEIS 217
           A P ++ PE D    I+ G+T      A++    E      + V +PTY G  ++L +I 
Sbjct: 127 AKPIFMHPEIDPKLGISHGITIQSVKKALE----EHSDAKGLLVINPTYFGFAADLEQIV 186

Query: 218 QICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGN 277
           Q+ HSY IP++VDEAHG H  F  ++P SA+Q G D+ A S HK+  SLTQSS+L++   
Sbjct: 187 QLAHSYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEG 246

Query: 278 IVDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKIS 337
           +V+ + V   +  L +TS SY+LLASLD AR +L+     +  + I LA   ++ IN I 
Sbjct: 247 LVNVKHVQSIISMLTTTSTSYILLASLDVARKRLATEGTALIEQTIQLAEHVRDAINSIE 306

Query: 338 GISILEVPIFSNSPAI--DPLRLTIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFV 397
            +      +         DP ++ +  + LG++G++A+  L + ++I  EL    +I  +
Sbjct: 307 HLYCPGKEMLGTDATFNYDPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNILCL 366

Query: 398 INLGTCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIV-LNPRDAFFAKKR 457
           I LG  E D   L++ ++D   LA+  R      +      P+I ++ L+PRDAF+++  
Sbjct: 367 ITLGDTESDTNTLIAALQD---LAATFRNRADKGVQVQVEIPEIPVLALSPRDAFYSETE 426

Query: 458 RENIKECVGKVCGELICPYPPGIPVTIPG 484
               +   G++  + +  YPPGIP+  PG
Sbjct: 427 VIPFENAAGRIIADFVMVYPPGIPIFTPG 448

BLAST of MC06g0377 vs. ExPASy Swiss-Prot
Match: P37536 (Uncharacterized protein YaaO OS=Bacillus subtilis (strain 168) OX=224308 GN=yaaO PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.0e-64
Identity = 177/490 (36.12%), Postives = 257/490 (52.45%), Query Frame = 0

Query: 38  PLVNALKISAEQNAARFHFPGHNRGRA----APSSFTQLIGLKPFMHDLPELPELDNLFC 97
           PL  AL   A +N+  FH PGH+ G      A S F  L+ +     D+ EL  LD+L  
Sbjct: 4   PLYKALIQHARRNSHSFHVPGHHNGDVFFDDAKSIFDPLLTI-----DVTELAGLDDLHH 63

Query: 98  PEGPILEAQQQAAKLFGAFETWFLVGGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISAL 157
           P G I EAQ+ A++L+G+ E++FLV GTT G    I++ C PG+ I++ RN H SV  A+
Sbjct: 64  PSGVIKEAQELASQLYGSAESFFLVNGTTVGNLAMILSVCEPGDTILVQRNCHKSVFHAV 123

Query: 158 VLSGAIPKYIMPEYDSNWDIAGGVTPSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNL 217
            LSGA P Y+ P+ DS    A  V      G IK++         + +T+PTY+G  ++L
Sbjct: 124 DLSGAEPVYLAPDVDS----AMHVPTHVPLGTIKEALEAYPDAKGLVLTNPTYYGHSADL 183

Query: 218 SEISQICHSYGIPLIVDEAHGAHFGFQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLH 277
           +EI    H YGIP++VDEAHGAHF      P SAL+ G D+V QS HK L ++T  S LH
Sbjct: 184 TEIITEAHHYGIPVLVDEAHGAHFILGEPFPVSALKMGADIVVQSAHKTLPAMTMGSYLH 243

Query: 278 MSGNI-VDRERVCRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNKAIDLANQAKNK 337
           ++ +  ++R+RV   L  LQS+SPSY ++ASLD ARA +    ++   K  D+  + +  
Sbjct: 244 LNSSCRINRDRVAEYLNRLQSSSPSYPIMASLDIARAYVQHIIEE--QKLSDILQRIETL 303

Query: 338 INKISGISILEVPIFSNSPAI-DPLRLTIGFQQLGLSGYEADEILHKNHDIVCELVGTQS 397
                 ++  E    +N   I DPL+LTI  ++ G SGY    IL +  +I  EL     
Sbjct: 304 KQTFDSLTNAEAVNPANPLIITDPLKLTIRSKR-GHSGYTLQSILER-ANIFTELADENQ 363

Query: 398 ITFVINLG----TCEDDIERLVSGIEDVSSLASILRIEGRSKLSTSAPFPDIKIVLNPRD 457
           +  V+ LG       + I  +   IE      + +  E   +  T  P+P  K VL+   
Sbjct: 364 VLLVLPLGGKRRINAEIIRSIDEEIEKTPPDQTFVSAEWGVQPVTVLPYP--KKVLHS-- 423

Query: 458 AFFAKKRRENIKECVGKVCGELICPYPPGIPVTIPGEVISEEVLDYLLHLKSKGASISGA 517
               KK   + +E  G++  E I PYPPGIP+ + GE I++E +  L  L S    + G 
Sbjct: 424 ---FKKEYVSFEEAAGRLNAEDIIPYPPGIPMIMAGERITKESVQKLSRLISMKTHVQGN 473

BLAST of MC06g0377 vs. NCBI nr
Match: XP_022134879.1 (uncharacterized protein LOC111007031 [Momordica charantia])

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 517/520 (99.42%), Postives = 517/520 (99.42%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN 60
           QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN
Sbjct: 38  QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN 97

Query: 61  RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG 120
           RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG
Sbjct: 98  RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG 157

Query: 121 TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS 180
           TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS
Sbjct: 158 TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS 217

Query: 181 QASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ 240
           Q   AIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ
Sbjct: 218 QVDKAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ 277

Query: 241 PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL 300
           PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL
Sbjct: 278 PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL 337

Query: 301 LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI 360
           LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI
Sbjct: 338 LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI 397

Query: 361 GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS 420
           GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS
Sbjct: 398 GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS 457

Query: 421 ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT 480
           ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT
Sbjct: 458 ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT 517

Query: 481 IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV
Sbjct: 518 IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 557

BLAST of MC06g0377 vs. NCBI nr
Match: XP_023515532.1 (uncharacterized protein LOC111779660 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 457/522 (87.55%), Postives = 484/522 (92.72%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 38  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 97

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIG KPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 98  HNGGRAAPSSFTQLIGFKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 157

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 158 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 217

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 218 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 277

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 278 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 337

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+KINKISGISILE P+FSN PAIDPLRL
Sbjct: 338 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKINKISGISILEFPMFSNFPAIDPLRL 397

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEADE ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 398 TIGFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 457

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFPD+K  LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 458 ASILRIEGRSKFSVSAPFPDVKTSLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 517

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+LSSLLVCNV
Sbjct: 518 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 558

BLAST of MC06g0377 vs. NCBI nr
Match: XP_023515530.1 (uncharacterized protein LOC111779660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515531.1 uncharacterized protein LOC111779660 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 457/522 (87.55%), Postives = 484/522 (92.72%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 39  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 98

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIG KPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 99  HNGGRAAPSSFTQLIGFKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 158

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 159 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 218

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 219 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 278

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 279 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 338

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+KINKISGISILE P+FSN PAIDPLRL
Sbjct: 339 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKINKISGISILEFPMFSNFPAIDPLRL 398

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEADE ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 399 TIGFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 458

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFPD+K  LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 459 ASILRIEGRSKFSVSAPFPDVKTSLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 518

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+LSSLLVCNV
Sbjct: 519 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 559

BLAST of MC06g0377 vs. NCBI nr
Match: KAG6589654.1 (hypothetical protein SDJN03_15077, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 455/522 (87.16%), Postives = 487/522 (93.30%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 39  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 98

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 99  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 158

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 159 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 218

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 219 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 278

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 279 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 338

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+K+NKISGISILE P+FSN PAIDPLRL
Sbjct: 339 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRL 398

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEADE ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 399 TIGFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 458

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK++ SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 459 ASILRIEGRSKVTVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 518

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGAS+SGASDP+LSSLLVCNV
Sbjct: 519 VMIPGEIISEEVLDYLLHLKSKGASLSGASDPKLSSLLVCNV 559

BLAST of MC06g0377 vs. NCBI nr
Match: XP_022921560.1 (uncharacterized protein LOC111429785 isoform X1 [Cucurbita moschata])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 455/522 (87.16%), Postives = 484/522 (92.72%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 39  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 98

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 99  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 158

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 159 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 218

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 219 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 278

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 279 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 338

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+K+NKISGISILE P+FSN PAIDPLRL
Sbjct: 339 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRL 398

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEAD  ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 399 TIGFQQLGLSGYEADATIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 458

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 459 ASILRIEGRSKFSVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 518

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+L SLLVCNV
Sbjct: 519 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLYSLLVCNV 559

BLAST of MC06g0377 vs. ExPASy TrEMBL
Match: A0A6J1C001 (uncharacterized protein LOC111007031 OS=Momordica charantia OX=3673 GN=LOC111007031 PE=3 SV=1)

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 517/520 (99.42%), Postives = 517/520 (99.42%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN 60
           QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN
Sbjct: 38  QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCPPLVNALKISAEQNAARFHFPGHN 97

Query: 61  RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG 120
           RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG
Sbjct: 98  RGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLVGG 157

Query: 121 TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS 180
           TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS
Sbjct: 158 TTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVTPS 217

Query: 181 QASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ 240
           Q   AIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ
Sbjct: 218 QVDKAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFGFQ 277

Query: 241 PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL 300
           PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL
Sbjct: 278 PQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLL 337

Query: 301 LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI 360
           LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI
Sbjct: 338 LASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRLTI 397

Query: 361 GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS 420
           GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS
Sbjct: 398 GFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSLAS 457

Query: 421 ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT 480
           ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT
Sbjct: 458 ILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIPVT 517

Query: 481 IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV
Sbjct: 518 IPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 557

BLAST of MC06g0377 vs. ExPASy TrEMBL
Match: A0A6J1E1Q8 (uncharacterized protein LOC111429785 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429785 PE=3 SV=1)

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 455/522 (87.16%), Postives = 484/522 (92.72%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 38  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 97

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 98  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 157

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 158 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 217

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 218 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 277

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 278 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 337

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+K+NKISGISILE P+FSN PAIDPLRL
Sbjct: 338 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRL 397

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEAD  ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 398 TIGFQQLGLSGYEADATIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 457

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 458 ASILRIEGRSKFSVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 517

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+L SLLVCNV
Sbjct: 518 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLYSLLVCNV 558

BLAST of MC06g0377 vs. ExPASy TrEMBL
Match: A0A6J1E647 (uncharacterized protein LOC111429785 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429785 PE=3 SV=1)

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 455/522 (87.16%), Postives = 484/522 (92.72%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKE--SCPPLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD SSKKQ++ K AI ESPIS+E  S  PLVNALK+SAE++AARFHFPG
Sbjct: 39  QETSIVKPKNNQDGSSKKQTSTK-AIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPG 98

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 99  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 158

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 159 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 218

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ   AI+D EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 219 PSQVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 278

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE VCRCLQTLQSTSPSY
Sbjct: 279 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSY 338

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK+K+NKISGISILE P+FSN PAIDPLRL
Sbjct: 339 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRL 398

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEAD  ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 399 TIGFQQLGLSGYEADATIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 458

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGELICPYPPGIP
Sbjct: 459 ASILRIEGRSKFSVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIP 518

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+L SLLVCNV
Sbjct: 519 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLYSLLVCNV 559

BLAST of MC06g0377 vs. ExPASy TrEMBL
Match: A0A6J1JG27 (uncharacterized protein LOC111484796 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484796 PE=3 SV=1)

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 451/522 (86.40%), Postives = 481/522 (92.15%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCP--PLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD  SKKQ++ K AI ESPIS+E     PLVNALK+SAE++AARFHFPG
Sbjct: 39  QETSIVKPKNNQDGPSKKQTSTK-AIGESPISQEGSRTLPLVNALKVSAEKDAARFHFPG 98

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 99  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 158

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 159 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 218

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ    IKD EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 219 PSQVDREIKDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 278

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE +CRCLQTLQSTSPSY
Sbjct: 279 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMICRCLQTLQSTSPSY 338

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK K+NK SGISIL+ P+FSN PAIDPLRL
Sbjct: 339 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKRKMNKTSGISILKFPMFSNFPAIDPLRL 398

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEADE ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 399 TIGFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 458

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGEL+CPYPPGIP
Sbjct: 459 ASILRIEGRSKFSVSAPFPNVKINLNPRDAFFSKKRRENIKECVGKVCGELVCPYPPGIP 518

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+LSSLLVCNV
Sbjct: 519 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 559

BLAST of MC06g0377 vs. ExPASy TrEMBL
Match: A0A6J1JDD3 (uncharacterized protein LOC111484796 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484796 PE=3 SV=1)

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 451/522 (86.40%), Postives = 481/522 (92.15%), Query Frame = 0

Query: 1   QETSIVEPKNNQDRSSKKQSTAKIAISESPISKESCP--PLVNALKISAEQNAARFHFPG 60
           QETSIV+PKNNQD  SKKQ++ K AI ESPIS+E     PLVNALK+SAE++AARFHFPG
Sbjct: 38  QETSIVKPKNNQDGPSKKQTSTK-AIGESPISQEGSRTLPLVNALKVSAEKDAARFHFPG 97

Query: 61  HNRGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGAFETWFLV 120
           HN GRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGA ETWFLV
Sbjct: 98  HNGGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLV 157

Query: 121 GGTTCGIQTAIMATCSPGEHIIIPRNSHVSVISALVLSGAIPKYIMPEYDSNWDIAGGVT 180
           GGTTCGIQ AIMATCSPG+HII+PRNSH+SVISALV+SGAIPKYIMPEYDSNWDIAGGVT
Sbjct: 158 GGTTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVT 217

Query: 181 PSQASGAIKDSEMEGHKVSAVFVTSPTYHGICSNLSEISQICHSYGIPLIVDEAHGAHFG 240
           PSQ    IKD EMEG K SAV VTSPTYHGICS+L EISQICH+ GIPLIVDEAHGAHFG
Sbjct: 218 PSQVDREIKDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFG 277

Query: 241 FQPQMPHSALQQGVDLVAQSTHKVLSSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSY 300
           FQPQ+P SALQQG DL  QSTHKVL SLTQSSMLHMSGNI+DRE +CRCLQTLQSTSPSY
Sbjct: 278 FQPQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMICRCLQTLQSTSPSY 337

Query: 301 LLLASLDAARAQLSDNPDKIFNKAIDLANQAKNKINKISGISILEVPIFSNSPAIDPLRL 360
           LLLASLDAARAQLSDNPDKIFN+AIDLA QAK K+NK SGISIL+ P+FSN PAIDPLRL
Sbjct: 338 LLLASLDAARAQLSDNPDKIFNRAIDLAYQAKRKMNKTSGISILKFPMFSNFPAIDPLRL 397

Query: 361 TIGFQQLGLSGYEADEILHKNHDIVCELVGTQSITFVINLGTCEDDIERLVSGIEDVSSL 420
           TIGFQQLGLSGYEADE ++KNH+IVCELVG QSITFVINLGTCEDDIERLVSGI+DVSS 
Sbjct: 398 TIGFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSF 457

Query: 421 ASILRIEGRSKLSTSAPFPDIKIVLNPRDAFFAKKRRENIKECVGKVCGELICPYPPGIP 480
           ASILRIEGRSK S SAPFP++KI LNPRDAFF+KKRRENIKECVGKVCGEL+CPYPPGIP
Sbjct: 458 ASILRIEGRSKFSVSAPFPNVKINLNPRDAFFSKKRRENIKECVGKVCGELVCPYPPGIP 517

Query: 481 VTIPGEVISEEVLDYLLHLKSKGASISGASDPQLSSLLVCNV 520
           V IPGE+ISEEVLDYLLHLKSKGASISGASDP+LSSLLVCNV
Sbjct: 518 VMIPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 558