MC06g0373 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0373
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tRNA pseudouridine synthase
Location	MC06: 2996533 .. 3003343 (+)
RNA-Seq Expression	MC06g0373
Synteny	MC06g0373

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGGCTTGAAGCTTACTTTCCTCGGCCCAAAACGAGCGACGTGAGTAAGTTCAGGCCTAGTCCTTCCAGCTGGACCAACATGGGCCTGTCCATTTTCATCTGCTCAACTGCAACATATTACGTGGATTTTACAGTCTCGTCGTTGCTGCGGCGGCGGCTGCACCTGCAACTGCAATACGGTCCCGGTCGTCCTCTCAGAGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGTTTTTCTTTCGTCCATCTACGTTATCCGCCAGTTTGTTTGATTTGAGATTTCTTTTGAAGTGTTTCGACTAAAATTCTGTTGCGATGAGAACTTCGCTTTTTTTTTTTTGTAGTAATTAAATCTGAATATGAGAAGGCTATCGATCAAGCTGTTCTTATGCTTTTGTGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGGTGAAGGTGAGTCTCCAATATCAACGTTTTCTTTAAACTTCTTTCTTCTTTCTGTATAAAGCAACAGCAATGTGAACCAGGACTATGTTTAATGCCACTTTCATTATAATGTTCTAACCGCGTTGGATAAGTTTTAACTACCTGGTGATATTAGTTTCTAGGGTTACTTTCTTTTGCTTCAAGCAAAGCAGTCCGAAGAGTAGTTTAAAACATTTATATGTCTTCTTTTTGCAATCAAGTTAGTAGAAAATAGAGAGGATAATTCATTTGTAGTACGGCTTCCTAAATCCTAAGGAATCGTATTTCTTTTTGTTCACATTATCTGGTCTTTAAAATAATGCTTGCCATCTAATACTTTCAATCGCTGTCACATTTTCCAGTGTGTGTTTGTTCTGAGTTTCGCTTGGATTTGGAGTTCTATTTAATCAATCATGTGCTACCAATTGTTTTATTTGATTAGTTGATCATAATCATTTTTTAAATATTGTTTTATTGGTTGATTAGAATTAGGAGAAATGAGTATGAAAATTTTGTTCCATAATTATCTTTCAGCACGAAAGAAGAAGGTATCATTGATTTATTTATTTATTGTGTATAGTACTTCATCCGCAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGGTAAGCTTGTTTCCTGAAACCACATGCACAAGTAATAAACATATACAATTGGAAGATTTTGAAGCTAGCGTTGTTCTTTTTAGTTTTTATTATAAAGTCTTTTAAATTGAAGAACCGATAAGTATTTGCAAGTGTTAAAAAAAGTTTGAGAAGTCAGTACTAGAGATACAATTGAATATCCCATACCTCCATTTCGAGTGAAACTTTCCTCTTGAGGACAATGTCCAGATTAGATCAATTGACCATATTAATCTCACCTTTCATGTATCTCCTTCCAGTAGTAGTATAACAGCTTCAAGTAATATCTTAATATCTTTTGATGTATAAATAGTTCGATTTCAGTCTAGAGATGGCAAAGACTCCTGTATATTGGAGGATCACGTCTATTTGCATGGATATCTGCTACTCTTTTTATTAAATTACTTTTGCATTGCCTCACATATCCTTCGGCAGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTGTATGTTAAATTTTTAAATCTTCGTTGACATGATTTTGTGCTTGTTTAGATATTTAATTCCACAACTTTGCTTTATTTTTCTTAGATTCTATCTCTAAATAAAAAACGAACTCTTAGATTACAATAAATTTTATCTATTTATATTATTTCTCGACAACTCTGATAGTTGCTGATGGATAACCATTAATTGCTTTGTCTCATCCGTTTGATATAAACTCCACTTTTGCGGTGCTTCACTAAAACACTCAAATTGATAACTCTGTTCCAACTGCTCTATACCCAAAGAATTGAAAGGTACGCTCAAAATACTTCTTAAAAGTGAGCAGTATTAAAACAGGAGTGGTGCATAGTCTATGTTTAATGTTATATAACTCGTTTCTTGATTAAAACCTCATAATTTTTTTCCTTTTAAATAATCAGTGGGAGGTGCAACACTGGAATTAAATCCAGGAAGGAAATTCCGATAAAAAGTAGCCAGAGTAAGATTTTTAATTTTTAAAATTTTAATTTTTAATATGAAACCATGCTTTGATTGAGAAAAATGAAAAGAAGAAAAACAAACAGCCCAGAGGACGACTACTACAAAAAAGGCTTAAATCCAAAAGAATAACACCTGACTGATAATTACAAAAGGCCCTGCCAAACCCCACTAGGAAGTATTAAACCTAAAGATTCCAAACAAACTCCACGGATCTCTCCACCCCTAAAAGTTATATTGTTGATAATTACAAAAGGCCCTAAAACCCCACTAGGAAGTATTAAACCTAATAAGATCCCAAACAAACTCCATGGATTTCTCCACCCCTAAAAGTTGTATTGTTCCTCTCCGGTCAAATACCTCATAAAGTAGCCAAAAACTTTGTACAACACCCACTGTATCTTAATTAGGCCAATCTTTAGGAGCTTATTGTTTAGAAGGTTTGACCTAGATACCCATCACTGATGATCAATTCAAGTAGTTCTGCAGCATGGAACTTGGAGTGCATTTCTTTATACCCTTTGCAGAAATCCTTATCTGATGCTATATGAAAAGGAAAGTTGGCATAGAAGATTAAATTTTCTCAATGCCAATAATAGAATTCAAGCCGGACATCCAAACTACTTGGACATTTGATGTACAAGGCTATGTTTTATTTTTTCTTTCGACACATTTGTCTCCAGACCATAGTTTTTTTACAATACTTATAACCCTTTATGTTAACTTCTGAATCTTGTCTGAACTGCGTTTCTTTTGGTTTGAGTTCTTTAACATAAATATATGTTATATGTTTCCTTATTAAGAAAAATGCAATTACAACATACCTTTGCTTTTATTTCTTCCACAAAAGTATTTGAGTTATTGCTATTTCTTTCCATCTTTTGTGTCAAATTTCCTTCGGAGGTGAACAGTTCTGTATCTCTTTCTGACTTCATCCACTTTATTTTTATTATTATTTTCTAATTTTAGAGAACCAATATCTGAATTCATTTAAACTCACATCACTTGTAGATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCAGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTAACATGCTGGTTTTCCATGAAATGTGGCATTGTAAATGAATGTGGTTCCACTGTCTAGCTCTAATATTGTTGCAGAATAACTTGGTGTTTATTTAACTTTCAATTTTGCTTGTCTCTCTCTTTCAAATACATGTAACTGCTCAATAACTTACTCCTGTAGGTGTCTCGTAACTTCCACCCAAATTTTTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGTGAGAACCATAAATTGAATCAACTATTGGCATTTTTTGTAGTTACTATAGCTTCTAGTTTGAGTATCATTATTAATTAGTTTTACTTTTGTTTCAATAATATTTATTATTTAATCCATTCTAATAAAAAAAATATTTGTTTTTAATTCTTATTGACTGTAAATCTTATTCAGTTGAATATAAGTTATTTCTTGGATTTTCTATTTACTAATAGCTTTCGATCAGAAATTTATGTAGATTTCATTGACTCATGTATGTCAAACTTTTGTTTTGCAATTGCGTTGCGTGTTTGACCGTGCCTTCCAATCAATATAAGTTTGACCTTGCTCTTTCTCCTGGCATGTATGGAGGTTTCTTCTAGTAACTTTTGACTTCATCGGTGTCTTCCCTTGATTCTTAAAGATTTGCTTCTGCTTCTATGGGGTCAATTCATTCAAAAAGAAAGCCGAGACTCATGGAGGTTATTTTTTCTTGGAAAATTTGGCTTGAAAGAAATAGGGGCAGTGTTCTCAGGAGTTGAGAAATGTTTAGAGGTTATTTGGGAGCTCATATTTTCCAATTCATTTTAGAGTACTAGCATTACAATTTCTGATCATTAAGTCTATCAAGCGTTTTCCTTCTATTCATTAGTCACTTTTTTGAATTTTTTTAGAATCTGTTTATAGGAAGGTGAAAATGGATGAATCCCATCTCTGCTATTTTCTAGGACCTGTGGATAGAAAGGAACAGCAGGTTTTTCTCGAGTAAAAATAAGGAAAAGTAGGAGACATGGTCAATGACAAAGTTAAGCATCAACTTGATATGCTTTGTTCAAGCCTGTGTACTTACCCACCTAATGAAGTCGACTCTTCCTTTTAACAGTTTTTTTAGGTCCTTCATTGGGGCTTCTGTCTCCCCTTTTGTACTTTTCATCATATCGATTTCTTTTGATTGTAGATATGATTAGGATAGCTAGTCTGAAGGCTGACTAAACAAAGTCAGTTATAAAGTTTACAAGTCAACTTTTCATTGATGTGATTGATGTGCTTGCTCGTATCTTTTTTCCTCCAACTGTCTTATCAACGACAGGATGATGTTCAGAACTTTCTGACGTGTACTAATGGCAACTATCCTCCTTTGCAGAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGTATTTTCTTACTTATGGTCATCTTCACGTTCTCCTTTTCTTTGATTATCTTTTTCCCTTTTATACTCCTCCCCTTGTTAAAATGAATTTGCAGAGTTGTAACATGTGATTTGCATTGTGTTTTCTACTGGGTCAGAACGTAGAGAACAGAAGATGAATATGTTAAAAAGTGAGTGTGAACCGAGGAAATGTAGAAGAAATTTACTTTGACATTATGTCATCAATGTGAACTCGTGAAGTACTTTAAAGAAAACTAGTTGTAGCATGTGCTCGATTCAATGCAGTTTCAGATTTTATTGACAATGGTTGCTCTCTCTTCTCTCAATATCTAAAATTGGATAACCTAAAAAATTTCAAAGATCATCTTTGAGTCTGAACCTCAACTTGGGAACAATGTGAACTTTAAGTAGAGTGCTTAATTGTTTATTTGGACTGATCTGTGGAACCTATGCTGCAACAGATGCACAAGTCCAAACTATATTGAACCAATATGAAGTTTATAATACGTTATAGAAATTATTGTAATAGAGATTAGGGTTTTGATTCTATGCTAGAAAAGCCAGGTTTTCAGCAAAACTACATCCCCCACAACTTGCGTGAGTGACATGCTTTTGAATGCAGCTACTCCAAATTTAGGTATCTGAATTAGTGGCGGTGTTGTATTTACAGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGGTATCATCTCCAGGCACCTATGCTAAAGAGTATATTGTACTGAACATAAAATTTGTTTATTTATATCAAAATCTTTCATTCCAACTGCTTAAACTTGAGAGCTAGCATCTTATATGTCAGAAAAGGAATTTCCCCAGTCTTAACAGCGTGATTAACCATAAGGCCGTGTGCAAATGAAATTGCTCTTTCATGTTTTTATGGCATTATTATGTCCTGTTGAATCCTTGATCTTTAGCAGTTTAACCAATCATAAAAGGATCGAATCATGACCTTGTAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGTAGCTGCTGGTGCAGGAGAGGATGCATTATTAAAGCTGATGGATGCCACATGTCGACGTGCCACCGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAACGACTGATATTTTTTCCCCTGGCTATTTCTGACCCATTTGATTGTTGTTGTAATCTAAGTACCTTCTAAATTTCTACAATTATAGATATAGTTATTGAATAGTGTTAGGAGAAATGTTCTGTTGAGAGTTTGAAAGTTAGTGTGCATTTTATCACTTTTTAGGTGTTTGTATGAGGTTGGGGCTCATTTTTCTACCAATTTACAACTATTTAAGAGCGTGTTTGAGAGTCATTTTACTTTTGTCGTCAAAACGACTTTCATTTGAAATGATGATGTTTGGCAAGTAATTTTGGAAGGATTACTTGCGGGATATTGCTGACATGAGCC

mRNA sequence

CTGGGCTTGAAGCTTACTTTCCTCGGCCCAAAACGAGCGACGTGAGTAAGTTCAGGCCTAGTCCTTCCAGCTGGACCAACATGGGCCTGTCCATTTTCATCTGCTCAACTGCAACATATTACGTGGATTTTACAGTCTCGTCGTTGCTGCGGCGGCGGCTGCACCTGCAACTGCAATACGGTCCCGGTCGTCCTCTCAGAGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGGTGAAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCAGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTGTCTCGTAACTTCCACCCAAATTTTTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGTAGCTGCTGGTGCAGGAGAGGATGCATTATTAAAGCTGATGGATGCCACATGTCGACGTGCCACCGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAACGACTGATATTTTTTCCCCTGGCTATTTCTGACCCATTTGATTGTTGTTGTAATCTAAGTACCTTCTAAATTTCTACAATTATAGATATAGTTATTGAATAGTGTTAGGAGAAATGTTCTGTTGAGAGTTTGAAAGTTAGTGTGCATTTTATCACTTTTTAGGTGTTTGTATGAGGTTGGGGCTCATTTTTCTACCAATTTACAACTATTTAAGAGCGTGTTTGAGAGTCATTTTACTTTTGTCGTCAAAACGACTTTCATTTGAAATGATGATGTTTGGCAAGTAATTTTGGAAGGATTACTTGCGGGATATTGCTGACATGAGCC

Coding sequence (CDS)

ATGGGCCTGTCCATTTTCATCTGCTCAACTGCAACATATTACGTGGATTTTACAGTCTCGTCGTTGCTGCGGCGGCGGCTGCACCTGCAACTGCAATACGGTCCCGGTCGTCCTCTCAGAGCGGTGGTTATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGGTGAAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCAGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTGTCTCGTAACTTCCACCCAAATTTTTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGTAGCTGCTGGTGCAGGAGAGGATGCATTATTAAAGCTGATGGATGCCACATGTCGACGTGCCACCGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAACGACTGA

Protein sequence

MGLSIFICSTATYYVDFTVSSLLRRRLHLQLQYGPGRPLRAVVMIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND

Homology

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BLAST of MC06g0373 vs. ExPASy Swiss-Prot
Match: C0QZT8 (tRNA pseudouridine synthase A OS=Brachyspira hyodysenteriae (strain ATCC 49526 / WA1) OX=565034 GN=truA PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.7e-13
Identity = 46/123 (37.40%), Postives = 64/123 (52.03%), Query Frame = 0


Query: 164 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 223
           KI + Y G  F GWQ QP L TVQG + K++ K   EK         + + GC    GRT
Sbjct: 5   KITIQYDGTDFYGWQIQPNLRTVQGEIYKAVQKVYGEK---------ITIYGC----GRT 64

Query: 224 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 283
           D GV AL QV +F   +  V    +  ++NS   R LR++   ++  +F+   SA +R Y
Sbjct: 65  DAGVHALGQVANFRVPKMLVPINKVHIALNSYLDRDLRIIKAEEMPDSFNARASATFREY 114

Query: 284 LYI 287
           LYI
Sbjct: 125 LYI 114

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BLAST of MC06g0373 vs. ExPASy Swiss-Prot
Match: B5YDX5 (tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=truA PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.2e-13
Identity = 51/134 (38.06%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 163 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGR 222
           +K+ LSY G  F G+QKQPG  TVQG +EK L         +LL D  + + G    AGR
Sbjct: 4   WKVELSYIGKDFWGFQKQPGKRTVQGELEKVL---------KLLFDEDIKVIG----AGR 63

Query: 223 TDKGVTALRQVCSFYTWRTDVKPLD-IEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWR 282
           TD GV AL QV +F T  +     D +   +N   P  +++  V  V  NFH  +SAK R
Sbjct: 64  TDAGVHALGQVVNFKTKESKNFSCDKLYKVLNKLLPGDIKIKKVEIVDDNFHARYSAKRR 123

Query: 283 RYLYIFPLKDGENM 296
            Y+Y+    + +N+
Sbjct: 124 WYIYVIYNNEEKNL 124

[Close]

BLAST of MC06g0373 vs. ExPASy Swiss-Prot
Match: B0SC03 (tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) OX=355278 GN=truA PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.9e-13
Identity = 44/123 (35.77%), Postives = 61/123 (49.59%), Query Frame = 0


Query: 163 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGR 222
           + +++ Y G  F+GWQKQ  L TVQ  +E +LG  +    A  L            VAGR
Sbjct: 4   YALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLS-----------VAGR 63

Query: 223 TDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRR 282
           TD GV AL  VC+F T         +  S+N+  P  + V +V +V  +FH  FS   R 
Sbjct: 64  TDTGVHALGMVCNFKTEHPIPNFHKLLVSLNALTPTGVSVKNVVEVPSDFHARFSCTGRE 115

Query: 283 YLY 286
           Y+Y
Sbjct: 124 YIY 115

[Close]

BLAST of MC06g0373 vs. ExPASy Swiss-Prot
Match: B0SJC4 (tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) OX=456481 GN=truA PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.9e-13
Identity = 44/123 (35.77%), Postives = 61/123 (49.59%), Query Frame = 0


Query: 163 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGR 222
           + +++ Y G  F+GWQKQ  L TVQ  +E +LG  +    A  L            VAGR
Sbjct: 4   YALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLS-----------VAGR 63

Query: 223 TDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRR 282
           TD GV AL  VC+F T         +  S+N+  P  + V +V +V  +FH  FS   R 
Sbjct: 64  TDTGVHALGMVCNFKTEHPIPNFHKLLVSLNALTPTGVSVKNVVEVPSDFHARFSCTGRE 115

Query: 283 YLY 286
           Y+Y
Sbjct: 124 YIY 115

[Close]

BLAST of MC06g0373 vs. ExPASy Swiss-Prot
Match: B5RLM0 (tRNA pseudouridine synthase A OS=Borrelia duttonii (strain Ly) OX=412419 GN=truA PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 6.4e-13
Identity = 45/131 (34.35%), Postives = 73/131 (55.73%), Query Frame = 0


Query: 167 LSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKG 226
           ++Y G+ + G+Q QP   T+QG +EK+L K +++ K ++              AGRTDKG
Sbjct: 8   IAYDGSLYHGFQIQPTKPTIQGEIEKALEK-INKTKVKIQS------------AGRTDKG 67

Query: 227 VTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYLYI 286
           V A RQ+ SFY  + ++   +++ +INS     +R++ +  VS  F P F AK R+Y Y 
Sbjct: 68  VHAKRQIISFYI-KININLKNLKTAINSLLKNDIRIIKLKYVSNEFQPRFHAKKRKYTYY 122

Query: 287 FPLKDGENMEP 298
             + + EN  P
Sbjct: 128 --ILNNENHYP 122

[Close]

BLAST of MC06g0373 vs. NCBI nr
Match: XP_022135239.1 (uncharacterized protein LOC111007251 isoform X1 [Momordica charantia])

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 442/443 (99.77%), Postives = 443/443 (100.00%), Query Frame = 0


Query: 44  MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 103
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 104 VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 163
           VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 164 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 223
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 224 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 283
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 284 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 343
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 344 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 403
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 404 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAPPAPP 463
           QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALL+LMDATCRRATAPPAPP
Sbjct: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLRLMDATCRRATAPPAPP 420

Query: 464 DGLSLVDVGYSEFDPQNCFILND 486
           DGLSLVDVGYSEFDPQNCFILND
Sbjct: 421 DGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of MC06g0373 vs. NCBI nr
Match: XP_038878348.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 791 bits (2043), Expect = 2.20e-286
Identity = 398/447 (89.04%), Postives = 414/447 (92.62%), Query Frame = 0


Query: 44  MIPNPRARLVS--SADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 103
           MIPNPRARLVS   A FG K LMENRA QKDYLHYNHTDRCRNARWTARESYQLMYGRPW
Sbjct: 1   MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 104 QEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 163
           QEVLDFYFHAVNGRFS SSLFGTE+   HDH+DAEIPLIYNT+ELNR SVKDKTGRWARA
Sbjct: 61  QEVLDFYFHAVNGRFSLSSLFGTEIG--HDHDDAEIPLIYNTAELNRVSVKDKTGRWARA 120

Query: 164 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 223
           TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVD+KKAQ+LKD+CLP+EGCAVVAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDDKKAQMLKDKCLPIEGCAVVAG 180

Query: 224 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWR 283
           RTDKGVTALRQVCSFYTWRTDV+P DIEDSIN AAP KLR LSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVQPQDIEDSINRAAPGKLRTLSVSQVSRIFHPNFSAKWR 240

Query: 284 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQN-EE 343
           RYLYIFPL DGENMEPD+AI ED E+CR+EENY KIE LNGE  KDEI DNLV   N EE
Sbjct: 241 RYLYIFPLNDGENMEPDYAIWEDVEDCRKEENYGKIEVLNGEECKDEIHDNLVFGPNDEE 300

Query: 344 LASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARL 403
           L S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARAVEARL
Sbjct: 301 LVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAVEARL 360

Query: 404 PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAP 463
           PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDALL+LMDATCRRATAP
Sbjct: 361 PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAP 420

Query: 464 PAPPDGLSLVDVGYSEFDPQNCFILND 486
           PAPPDGLSLVDVGY+EFD QNCFILND
Sbjct: 421 PAPPDGLSLVDVGYTEFDSQNCFILND 445

[Close]

BLAST of MC06g0373 vs. NCBI nr
Match: XP_022135240.1 (uncharacterized protein LOC111007251 isoform X2 [Momordica charantia])

HSP 1 Score: 778 bits (2009), Expect = 3.32e-282
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 44  MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 103
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 104 VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 163
           VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 164 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 223
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 224 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 283
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 284 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 343
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 344 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 403
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 404 QGVGRSVMCVELVANRFLRK 423
           QGVGRSVMCVELVANRFLRK
Sbjct: 361 QGVGRSVMCVELVANRFLRK 380

[Close]

BLAST of MC06g0373 vs. NCBI nr
Match: XP_023006334.1 (uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima])

HSP 1 Score: 755 bits (1949), Expect = 2.52e-271
Identity = 388/462 (83.98%), Postives = 412/462 (89.18%), Query Frame = 0


Query: 37  RPLR---AVVMIPNPRARLVSSAD--------FGCKFLMENRAPQKDYLHYNHTDRCRNA 96
           RPLR   AVVM+PNPRARL+S +         FG K LMENRA +KDYLHYNHTDRCRNA
Sbjct: 33  RPLRLGGAVVMLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNA 92

Query: 97  RWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSE 156
           RWT+RESYQLMY RPWQEVLDFY HAVNGR S SSL     +TV DH+D EI  + NT+E
Sbjct: 93  RWTSRESYQLMYERPWQEVLDFYSHAVNGRLSLSSL---RTETVRDHDDTEIQPVCNTAE 152

Query: 157 LNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQL 216
           LNRA VKD TGRWARATFKIVLSYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ 
Sbjct: 153 LNRAPVKDGTGRWARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQ 212

Query: 217 LKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSV 276
           LKDRCLP+EGCAVVAGRTDKGVTALRQVCSFYTWRTDVKP DIE SIN+AAP KLRVLSV
Sbjct: 213 LKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSV 272

Query: 277 SQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFK 336
           SQVSR FHPNFSAKWRRYLYIFPLKDGENMEP+FAI ED EN REEE  +KIE L+GE K
Sbjct: 273 SQVSRMFHPNFSAKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECK 332

Query: 337 DEILDNLVLSQN-EELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPP 396
           DEIL+NL+LSQ+ EE AS +KPQTFS+ RVN+LLRKLEGKLLSYKMFARDTKASRNEGPP
Sbjct: 333 DEILNNLILSQDDEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPP 392

Query: 397 TECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDA 456
           TECF+YHARA+EARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDA
Sbjct: 393 TECFVYHARAMEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDA 452

Query: 457 LLKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND 486
           LLKLMDATCRRATAPPAPPDGLSLVDVGY+EFDPQNCFILND
Sbjct: 453 LLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDPQNCFILND 491

[Close]

BLAST of MC06g0373 vs. NCBI nr
Match: KAG7021669.1 (truA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 754 bits (1947), Expect = 4.38e-271
Identity = 398/494 (80.57%), Postives = 425/494 (86.03%), Query Frame = 0


Query: 1   MGLSIFICSTATYYVDFTVSSLLRRRLHLQLQYGPGRPLR---AVVMIPNPRARLVSSAD 60
           MGL  +IC TAT    + +          +      RPLR   AVVMIPNPRA L+SS+ 
Sbjct: 1   MGLLSYICLTATSTWIYIIVPAAPAAATAKYS----RPLRLGGAVVMIPNPRAILLSSSG 60

Query: 61  ----FGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVN 120
               FG K LMENRA +KDYLHYNHTDRCRNARWT+RESYQ MY RPWQEVLDFY HAVN
Sbjct: 61  AGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYERPWQEVLDFYSHAVN 120

Query: 121 GRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATFKIVLSYHGNS 180
           GR S SSL GTE  TV DH+D EI  + NT+EL+RA VKD TGRWARATFKIVLSYHGNS
Sbjct: 121 GRLSLSSL-GTE--TVRDHDDTEIQPVCNTAELDRAPVKDGTGRWARATFKIVLSYHGNS 180

Query: 181 FDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQV 240
           FDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLP+EGCAVVAGRTDKGVTALRQV
Sbjct: 181 FDGWQKQPDLNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAGRTDKGVTALRQV 240

Query: 241 CSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGE 300
           CSFYTWRTDVKP DIE SIN+AAP KLRVLSVSQVSR FHPNFSAKWRRYLYIFPLKDGE
Sbjct: 241 CSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLKDGE 300

Query: 301 NMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQN-EELASDDKPQTFSVR 360
            MEP+FAI ED EN REEE+ +KIEAL+GE KDEIL+NLVLSQ+ EE A  +KPQTFS+ 
Sbjct: 301 KMEPNFAIWEDVENSREEEHRDKIEALHGECKDEILNNLVLSQDDEEPALANKPQTFSIM 360

Query: 361 RVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLDQGVGRSVMC 420
           RVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA+EARLPCLDQGVGRSVMC
Sbjct: 361 RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQGVGRSVMC 420

Query: 421 VELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVG 480
           VELVANRFLRKMVRVLVATAIRE AAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVG
Sbjct: 421 VELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVG 480

Query: 481 YSEFDPQNCFILND 486
           Y+EFDPQNCFILND
Sbjct: 481 YTEFDPQNCFILND 487

[Close]

BLAST of MC06g0373 vs. ExPASy TrEMBL
Match: A0A6J1C0W1 (tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 442/443 (99.77%), Postives = 443/443 (100.00%), Query Frame = 0


Query: 44  MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 103
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 104 VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 163
           VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 164 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 223
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 224 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 283
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 284 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 343
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 344 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 403
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 404 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAPPAPP 463
           QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALL+LMDATCRRATAPPAPP
Sbjct: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLRLMDATCRRATAPPAPP 420

Query: 464 DGLSLVDVGYSEFDPQNCFILND 486
           DGLSLVDVGYSEFDPQNCFILND
Sbjct: 421 DGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of MC06g0373 vs. ExPASy TrEMBL
Match: A0A6J1C0L5 (uncharacterized protein LOC111007251 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007251 PE=4 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 1.61e-282
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 44  MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 103
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 104 VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 163
           VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 164 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 223
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 224 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 283
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 284 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 343
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 344 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 403
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 404 QGVGRSVMCVELVANRFLRK 423
           QGVGRSVMCVELVANRFLRK
Sbjct: 361 QGVGRSVMCVELVANRFLRK 380

[Close]

BLAST of MC06g0373 vs. ExPASy TrEMBL
Match: A0A6J1L1W3 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 755 bits (1949), Expect = 1.22e-271
Identity = 388/462 (83.98%), Postives = 412/462 (89.18%), Query Frame = 0


Query: 37  RPLR---AVVMIPNPRARLVSSAD--------FGCKFLMENRAPQKDYLHYNHTDRCRNA 96
           RPLR   AVVM+PNPRARL+S +         FG K LMENRA +KDYLHYNHTDRCRNA
Sbjct: 33  RPLRLGGAVVMLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNA 92

Query: 97  RWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSE 156
           RWT+RESYQLMY RPWQEVLDFY HAVNGR S SSL     +TV DH+D EI  + NT+E
Sbjct: 93  RWTSRESYQLMYERPWQEVLDFYSHAVNGRLSLSSL---RTETVRDHDDTEIQPVCNTAE 152

Query: 157 LNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQL 216
           LNRA VKD TGRWARATFKIVLSYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ 
Sbjct: 153 LNRAPVKDGTGRWARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQ 212

Query: 217 LKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSV 276
           LKDRCLP+EGCAVVAGRTDKGVTALRQVCSFYTWRTDVKP DIE SIN+AAP KLRVLSV
Sbjct: 213 LKDRCLPIEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSV 272

Query: 277 SQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFK 336
           SQVSR FHPNFSAKWRRYLYIFPLKDGENMEP+FAI ED EN REEE  +KIE L+GE K
Sbjct: 273 SQVSRMFHPNFSAKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECK 332

Query: 337 DEILDNLVLSQN-EELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPP 396
           DEIL+NL+LSQ+ EE AS +KPQTFS+ RVN+LLRKLEGKLLSYKMFARDTKASRNEGPP
Sbjct: 333 DEILNNLILSQDDEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPP 392

Query: 397 TECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDA 456
           TECF+YHARA+EARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDA
Sbjct: 393 TECFVYHARAMEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDA 452

Query: 457 LLKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND 486
           LLKLMDATCRRATAPPAPPDGLSLVDVGY+EFDPQNCFILND
Sbjct: 453 LLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDPQNCFILND 491

[Close]

BLAST of MC06g0373 vs. ExPASy TrEMBL
Match: A0A5A7UXE7 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004790 PE=3 SV=1)

HSP 1 Score: 751 bits (1940), Expect = 4.97e-271
Identity = 387/447 (86.58%), Postives = 403/447 (90.16%), Query Frame = 0


Query: 44  MIPNPRARLVS--SADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 103
           MIPNPRARLVS  SA FG K LMENRA QKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 104 QEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 163
           QEVLDFY HAVNGR S SSL GTE+    DH+DAE PLIYN++ELNRA+VKDKTGRWARA
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTEI--CRDHDDAETPLIYNSAELNRAAVKDKTGRWARA 120

Query: 164 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 223
            FKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKD+CLPLEGCAVVAG
Sbjct: 121 NFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCAVVAG 180

Query: 224 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWR 283
           RTDKGVTALRQVCSFYTWRTDVKP DIEDSINSAAP KLR LSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWR 240

Query: 284 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQNE-E 343
           RYLYI PL DGENMEPD AI ED EN REEEN  KIEALNGE   D I DNLVLS N+ E
Sbjct: 241 RYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNGEKCVDGIHDNLVLSPNDDE 300

Query: 344 LASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARL 403
           L S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA E RL
Sbjct: 301 LVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRL 360

Query: 404 PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAP 463
           PCL+QGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDALL+LM ATCRRATAP
Sbjct: 361 PCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMVATCRRATAP 420

Query: 464 PAPPDGLSLVDVGYSEFDPQNCFILND 486
           PAPPDGLSLVDVGY+EFD Q CFILND
Sbjct: 421 PAPPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of MC06g0373 vs. ExPASy TrEMBL
Match: A0A1S3BWI6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1)

HSP 1 Score: 751 bits (1940), Expect = 4.97e-271
Identity = 387/447 (86.58%), Postives = 403/447 (90.16%), Query Frame = 0


Query: 44  MIPNPRARLVS--SADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 103
           MIPNPRARLVS  SA FG K LMENRA QKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 104 QEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 163
           QEVLDFY HAVNGR S SSL GTE+    DH+DAE PLIYN++ELNRA+VKDKTGRWARA
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTEI--CRDHDDAETPLIYNSAELNRAAVKDKTGRWARA 120

Query: 164 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 223
            FKIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKD+CLPLEGCAVVAG
Sbjct: 121 NFKIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCAVVAG 180

Query: 224 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWR 283
           RTDKGVTALRQVCSFYTWRTDVKP DIEDSINSAAP KLR LSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWR 240

Query: 284 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQNE-E 343
           RYLYI PL DGENMEPD AI ED EN REEEN  KIEALNGE   D I DNLVLS N+ E
Sbjct: 241 RYLYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNGEKCVDGIHDNLVLSPNDDE 300

Query: 344 LASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARL 403
           L S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA E RL
Sbjct: 301 LVSADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRL 360

Query: 404 PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAP 463
           PCL+QGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDALL+LM ATCRRATAP
Sbjct: 361 PCLEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMVATCRRATAP 420

Query: 464 PAPPDGLSLVDVGYSEFDPQNCFILND 486
           PAPPDGLSLVDVGY+EFD Q CFILND
Sbjct: 421 PAPPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of MC06g0373 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.0e-142
Identity = 259/421 (61.52%), Postives = 316/421 (75.06%), Query Frame = 0


Query: 73  YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTEVKTVHDH 132
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S  SLF  +   VHD 
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 133 NDAEIPLIYNTSELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVE 192
              E  L+   SE   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQG+VE
Sbjct: 66  GITEEMLL--ESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVE 125

Query: 193 KSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDS 252
           KSLG FVDE+KAQLLK +C PLEG  +VAGRTDKGV+AL QVCSFYTWR D++P+ IED+
Sbjct: 126 KSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDA 185

Query: 253 INSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREE 312
           IN  A  KLRV+S+S+VSR+FHPNFSAKWRRYLYIFPL D E +      GE+ EN   +
Sbjct: 186 INKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKS---GENHENFIFD 245

Query: 313 ENYNKIEALNGEFKDEILDNLVLSQNEELASD---------DKPQTFSVRRVNRLLRKLE 372
           E  NK +  NG   +EI + +++S+++EL  +         +KP  FSV +V++LL+ L+
Sbjct: 246 E--NKKKQRNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQ 305

Query: 373 GKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLR 432
           GK+LSYKMFARDTKA+RNEGPPTECF+YHARA E RL   D   GR VMCVELVANRFLR
Sbjct: 306 GKVLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSDCVEGRRVMCVELVANRFLR 365

Query: 433 KMVRVLVATAIREVAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCF 484
           KMVRVLVAT+IRE AAGA +DALLKL++A+CRRATAPPAP +GL L DVGY++FDPQ   
Sbjct: 366 KMVRVLVATSIREAAAGAEDDALLKLLEASCRRATAPPAPSEGLCLFDVGYTDFDPQTSL 419

[Close]

BLAST of MC06g0373 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.3e-135
Identity = 259/472 (54.87%), Postives = 316/472 (66.95%), Query Frame = 0


Query: 73  YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTEVKTVHDH 132
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S  SLF  +   VHD 
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 133 NDAEIPLIYNTSELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVE 192
              E  L+   SE   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQG+VE
Sbjct: 66  GITEEMLL--ESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVE 125

Query: 193 KSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDS 252
           KSLG FVDE+KAQLLK +C PLEG  +VAGRTDKGV+AL QVCSFYTWR D++P+ IED+
Sbjct: 126 KSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDA 185

Query: 253 INSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREE 312
           IN  A  KLRV+S+S+VSR+FHPNFSAKWRRYLYIFPL D E +      GE+ EN   +
Sbjct: 186 INKDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKS---GENHENFIFD 245

Query: 313 ENYNKIEALNGEFKDEILDNLVLSQNEELASD---------DKPQTFSVRRVNRLLRKLE 372
           E  NK +  NG   +EI + +++S+++EL  +         +KP  FSV +V++LL+ L+
Sbjct: 246 E--NKKKQRNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQ 305

Query: 373 GKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLP---------------------- 432
           GK+LSYKMFARDTKA+RNEGPPTECF+YHARA E RL                       
Sbjct: 306 GKVLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSVVHFVAWVFGGSIVLVILAT 365

Query: 433 -----------------------------CLDQGVGRSVMCVELVANRFLRKMVRVLVAT 484
                                          D   GR VMCVELVANRFLRKMVRVLVAT
Sbjct: 366 EGSTLDLTDTKLYIASWFLIKRSCEFLFIASDCVEGRRVMCVELVANRFLRKMVRVLVAT 425

[Close]

BLAST of MC06g0373 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.1e-10
Identity = 38/123 (30.89%), Postives = 58/123 (47.15%), Query Frame = 0


Query: 163 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGR 222
           +++V++Y G  F GWQ Q    T+Q ++EK+L +  +  + +L            + AGR
Sbjct: 43  WRLVIAYDGTRFAGWQYQESPPTIQSMLEKALIQITELGRKELQ----------LIGAGR 102

Query: 223 TDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRR 282
           TD GV A  QV  F T            ++N   P+ +RV  +S     FH  FSA  + 
Sbjct: 103 TDAGVHAWGQVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKV 155

Query: 283 YLY 286
           Y Y
Sbjct: 163 YRY 155

[Close]

BLAST of MC06g0373 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 6.8e-10
Identity = 48/167 (28.74%), Postives = 77/167 (46.11%), Query Frame = 0


Query: 157 RWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSL---GKFVDEKKAQLLKDRCLPL 216
           ++ R    IV ++ G  + G QK PG  T++G +E++L   G   D        DR  P 
Sbjct: 54  KYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPD-------ADRNKPR 113

Query: 217 EGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFH 276
                 + RTDKGV+A+ QV S    R  V P    + +NS  P ++RV    +V+ +F 
Sbjct: 114 NYEWARSARTDKGVSAVGQVVS---GRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFS 173

Query: 277 PNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEAL 321
                  RRY+Y+ P+        D  +  +AE  R +  Y  ++ +
Sbjct: 174 SKKFCDRRRYVYLIPV-----FALDPCVHGEAEMVRTDLGYEYVKCV 205

[Close]

BLAST of MC06g0373 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 62.8 bits (151), Expect = 8.9e-10
Identity = 56/209 (26.79%), Postives = 92/209 (44.02%), Query Frame = 0


Query: 165 IVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTD 224
           I+ ++ G  + G QK PG  T++G +E++L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 225 KGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRYL 284
           KGV+A+ QV S    R  V PL   + +NS  P ++R+     V+ +F        RRY+
Sbjct: 86  KGVSAVGQVVS---GRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYV 145

Query: 285 YIFPL--------KDGENMEPDFAIGEDAENCRE-EENYNKI-EALNGEFKDEILDNLVL 344
           Y+ P+        +D E +      GE+   C E  E   KI   L G++K        L
Sbjct: 146 YLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWKGTNFGTKSL 205

Query: 345 SQNEELASDDKPQTFSVRRVNRLLRKLEG 364
               +++S++     S  ++  L   L G
Sbjct: 206 DFQSDISSNNSSALRSDIKIEALSSNLAG 227

The following BLAST results are available for this feature:

BLAST of MC06g0373 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0QZT8	1.7e-13	37.40	tRNA pseudouridine synthase A OS=Brachyspira hyodysenteriae (strain ATCC 49526 /...	[more]
B5YDX5	2.2e-13	38.06	tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / ...	[more]
B0SC03	4.9e-13	35.77	tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc ...	[more]
B0SJC4	4.9e-13	35.77	tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc ...	[more]
B5RLM0	6.4e-13	34.35	tRNA pseudouridine synthase A OS=Borrelia duttonii (strain Ly) OX=412419 GN=truA...	[more]

BLAST of MC06g0373 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135239.1	0.0	99.77	uncharacterized protein LOC111007251 isoform X1 [Momordica charantia]	[more]
XP_038878348.1	2.20e-286	89.04	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_022135240.1	3.32e-282	100.00	uncharacterized protein LOC111007251 isoform X2 [Momordica charantia]	[more]
XP_023006334.1	2.52e-271	83.98	uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima]	[more]
KAG7021669.1	4.38e-271	80.57	truA, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC06g0373 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0W1	0.0	99.77	tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 ...	[more]
A0A6J1C0L5	1.61e-282	100.00	uncharacterized protein LOC111007251 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1L1W3	1.22e-271	83.98	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=...	[more]
A0A5A7UXE7	4.97e-271	86.58	tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A1S3BWI6	4.97e-271	86.58	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1	[more]

BLAST of MC06g0373 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35400.1	2.0e-142	61.52	Pseudouridine synthase family protein	[more]
AT5G35400.2	2.3e-135	54.87	Pseudouridine synthase family protein	[more]
AT3G06950.1	3.1e-10	30.89	Pseudouridine synthase family protein	[more]
AT1G20370.1	6.8e-10	28.74	Pseudouridine synthase family protein	[more]
AT1G76120.1	8.9e-10	26.79	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 327..471 e-value: 6.8E-14 score: 53.8
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 383..473 e-value: 1.3E-6 score: 28.9
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 160..278 e-value: 4.2E-25 score: 90.1
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 80..483
None	No IPR available	PANTHER	PTHR11142:SF10	TRNA PSEUDOURIDINE SYNTHASE	coord: 80..483
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 162..478

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0373.1	MC06g0373.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity