MC06g0301 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0301
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Eukaryotic aspartyl protease family protein
Location	MC06: 2441040 .. 2442500 (+)
RNA-Seq Expression	MC06g0301
Synteny	MC06g0301

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAATGAACATGGCCCTTCTCTTCCTCTTCCTCATCATCTTCACTTCCTCTCTGTTCTCTCCTACTCTGTCTCGCACCTTACTGGGTCCTTCCCATACCCACACGCACACGCACACGACCGCCATTTTCGATGTCTCTGCTTCCTCAAAGCGAGCCCAAAACGCCCTTTCCATTCATCCCTCTCAATTCCGCAATAATCTCTCTCAAAATTCTTCCTTTTCTCTGCCATTGCACCCCAGATTGGCTCTTCACAACCCCTCCTACAAGGACTACCAGAGCTTGGTCCGGGCCCGCCTCGCCCGGGATGCTGCCCGGGTTCAATCTCTGAACCAAAATCTACACCGCGCTTTAAATGGGGGAACTCCCGGGTCTGACTCTGGGATCCGACCCGATGCAGTTACGGTCCCGGTTGTTTCTGGACAGAGCCAAGGGAGTGGAGAATATTTTGCCCGAATTGGCGTGGGGCAGCCTGCGCAATCGTTCTTCCTGGTGCCCGACACCGGCAGCGATATCACGTGGCTCCAGTGCAAGTCCTGCGACGATTCGGGTTCCTGCTTCAAACAATCCGACCCGATATTTGACCCGAGTTCGTCCTCCTCTTACAGCTCCCTCTCCTGCGATTCCCAACAATGTAAATCTCTGGACCGAGCCGGGTGCCAATCCGGCACGTGCCTCTATCAGGTCTCGTACGGCGACGGCTCATTCACCACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGGGAGCTCCAATTCCATCTCCAACGTCGCCATCGGCTGCGGCCACGACAACGAAGGGCTTTTCGTCGGAGCCGCCGGTTTGATCGGGCTCGGAGGCGCGGCGCTCTCTCTGTCCTCCCAAATCAAAGCGTCGTCGTTTTCGTACTGCCTCGTGGACCGTGACTCGGACTCATCCTCCACTCTGGAGTTCAACTCGGGACGACCCGGTAACTCGGTCACCACCCCGCTACTCGTGAACCGGAAATTCAAATCTTACCGGTACGTGGAGGTGACGGGGCTGAGCGTCGGGGGGCAGCCGCTGCCGATATCGTCGACGAGATTTGAAATCGACGGCTCGGGACTCGGGGGGATAATCGTGGACTCGGGGACCACCATAACTCGGCTGCCGAGTGACGTGTACGACGCGCTCAAAGGCGCGTTTGTGAAACTGACCGGGAACCTGCCGCCGGCGGCAGGGATATCGCCGTTCGACACGTGCTACGATTTGTCGTCGCAGTCGAGCGTGGACGTGCCGACGGTGTCGTTTCAACTGTCAGAAGGGAAATCGTTGCGGCTTCCGGCGAATAACTACTTGATACGGATGGACTCGGCGGGAACGTACTGCTTGGCGTTTCTGCCCACAACGACGTCGCTTTCGATCATCGGGAACTTCCAACAGCAGGGAATACGTGTCAGCTACGACCTGGTCAACTCTCTCGTCGGATTCTCCTCCAATCAGTGT

mRNA sequence

ATAATGAACATGGCCCTTCTCTTCCTCTTCCTCATCATCTTCACTTCCTCTCTGTTCTCTCCTACTCTGTCTCGCACCTTACTGGGTCCTTCCCATACCCACACGCACACGCACACGACCGCCATTTTCGATGTCTCTGCTTCCTCAAAGCGAGCCCAAAACGCCCTTTCCATTCATCCCTCTCAATTCCGCAATAATCTCTCTCAAAATTCTTCCTTTTCTCTGCCATTGCACCCCAGATTGGCTCTTCACAACCCCTCCTACAAGGACTACCAGAGCTTGGTCCGGGCCCGCCTCGCCCGGGATGCTGCCCGGGTTCAATCTCTGAACCAAAATCTACACCGCGCTTTAAATGGGGGAACTCCCGGGTCTGACTCTGGGATCCGACCCGATGCAGTTACGGTCCCGGTTGTTTCTGGACAGAGCCAAGGGAGTGGAGAATATTTTGCCCGAATTGGCGTGGGGCAGCCTGCGCAATCGTTCTTCCTGGTGCCCGACACCGGCAGCGATATCACGTGGCTCCAGTGCAAGTCCTGCGACGATTCGGGTTCCTGCTTCAAACAATCCGACCCGATATTTGACCCGAGTTCGTCCTCCTCTTACAGCTCCCTCTCCTGCGATTCCCAACAATGTAAATCTCTGGACCGAGCCGGGTGCCAATCCGGCACGTGCCTCTATCAGGTCTCGTACGGCGACGGCTCATTCACCACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGGGAGCTCCAATTCCATCTCCAACGTCGCCATCGGCTGCGGCCACGACAACGAAGGGCTTTTCGTCGGAGCCGCCGGTTTGATCGGGCTCGGAGGCGCGGCGCTCTCTCTGTCCTCCCAAATCAAAGCGTCGTCGTTTTCGTACTGCCTCGTGGACCGTGACTCGGACTCATCCTCCACTCTGGAGTTCAACTCGGGACGACCCGGTAACTCGGTCACCACCCCGCTACTCGTGAACCGGAAATTCAAATCTTACCGGTACGTGGAGGTGACGGGGCTGAGCGTCGGGGGGCAGCCGCTGCCGATATCGTCGACGAGATTTGAAATCGACGGCTCGGGACTCGGGGGGATAATCGTGGACTCGGGGACCACCATAACTCGGCTGCCGAGTGACGTGTACGACGCGCTCAAAGGCGCGTTTGTGAAACTGACCGGGAACCTGCCGCCGGCGGCAGGGATATCGCCGTTCGACACGTGCTACGATTTGTCGTCGCAGTCGAGCGTGGACGTGCCGACGGTGTCGTTTCAACTGTCAGAAGGGAAATCGTTGCGGCTTCCGGCGAATAACTACTTGATACGGATGGACTCGGCGGGAACGTACTGCTTGGCGTTTCTGCCCACAACGACGTCGCTTTCGATCATCGGGAACTTCCAACAGCAGGGAATACGTGTCAGCTACGACCTGGTCAACTCTCTCGTCGGATTCTCCTCCAATCAGTGT

Coding sequence (CDS)

ATAATGAACATGGCCCTTCTCTTCCTCTTCCTCATCATCTTCACTTCCTCTCTGTTCTCTCCTACTCTGTCTCGCACCTTACTGGGTCCTTCCCATACCCACACGCACACGCACACGACCGCCATTTTCGATGTCTCTGCTTCCTCAAAGCGAGCCCAAAACGCCCTTTCCATTCATCCCTCTCAATTCCGCAATAATCTCTCTCAAAATTCTTCCTTTTCTCTGCCATTGCACCCCAGATTGGCTCTTCACAACCCCTCCTACAAGGACTACCAGAGCTTGGTCCGGGCCCGCCTCGCCCGGGATGCTGCCCGGGTTCAATCTCTGAACCAAAATCTACACCGCGCTTTAAATGGGGGAACTCCCGGGTCTGACTCTGGGATCCGACCCGATGCAGTTACGGTCCCGGTTGTTTCTGGACAGAGCCAAGGGAGTGGAGAATATTTTGCCCGAATTGGCGTGGGGCAGCCTGCGCAATCGTTCTTCCTGGTGCCCGACACCGGCAGCGATATCACGTGGCTCCAGTGCAAGTCCTGCGACGATTCGGGTTCCTGCTTCAAACAATCCGACCCGATATTTGACCCGAGTTCGTCCTCCTCTTACAGCTCCCTCTCCTGCGATTCCCAACAATGTAAATCTCTGGACCGAGCCGGGTGCCAATCCGGCACGTGCCTCTATCAGGTCTCGTACGGCGACGGCTCATTCACCACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGGGAGCTCCAATTCCATCTCCAACGTCGCCATCGGCTGCGGCCACGACAACGAAGGGCTTTTCGTCGGAGCCGCCGGTTTGATCGGGCTCGGAGGCGCGGCGCTCTCTCTGTCCTCCCAAATCAAAGCGTCGTCGTTTTCGTACTGCCTCGTGGACCGTGACTCGGACTCATCCTCCACTCTGGAGTTCAACTCGGGACGACCCGGTAACTCGGTCACCACCCCGCTACTCGTGAACCGGAAATTCAAATCTTACCGGTACGTGGAGGTGACGGGGCTGAGCGTCGGGGGGCAGCCGCTGCCGATATCGTCGACGAGATTTGAAATCGACGGCTCGGGACTCGGGGGGATAATCGTGGACTCGGGGACCACCATAACTCGGCTGCCGAGTGACGTGTACGACGCGCTCAAAGGCGCGTTTGTGAAACTGACCGGGAACCTGCCGCCGGCGGCAGGGATATCGCCGTTCGACACGTGCTACGATTTGTCGTCGCAGTCGAGCGTGGACGTGCCGACGGTGTCGTTTCAACTGTCAGAAGGGAAATCGTTGCGGCTTCCGGCGAATAACTACTTGATACGGATGGACTCGGCGGGAACGTACTGCTTGGCGTTTCTGCCCACAACGACGTCGCTTTCGATCATCGGGAACTTCCAACAGCAGGGAATACGTGTCAGCTACGACCTGGTCAACTCTCTCGTCGGATTCTCCTCCAATCAGTGT

Protein sequence

IMNMALLFLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC

Homology

[Close]

BLAST of MC06g0301 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.3e-126
Identity = 249/503 (49.50%), Postives = 340/503 (67.59%), Query Frame = 0


Query: 11  LIIFTSSLFSPTL---SRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 70
           L + T SLF  T    SR+L  P  T+       + DV +S ++ Q  LS+ P+  R++L
Sbjct: 10  LAVVTLSLFLTTTDASSRSLSTPPKTN-------VLDVVSSLQQTQTILSLDPT--RSSL 69

Query: 71  --------------SQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNL 130
                         + +S  SL LH R       +KDY+SL  +RL RD++RV  +   +
Sbjct: 70  TTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 129

Query: 131 HRALNG-------GTPGSDSGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPD 190
             A+ G            D+  + + +T PVVSG SQGSGEYF+RIGVG PA+  +LV D
Sbjct: 130 RFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLD 189

Query: 191 TGSDITWLQCKSCDDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLY 250
           TGSD+ W+QC+ C D   C++QSDP+F+P+SSS+Y SL+C + QC  L+ + C+S  CLY
Sbjct: 190 TGSDVNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLY 249

Query: 251 QVSYGDGSFTTGEFATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSS 310
           QVSYGDGSFT GE AT+T++FG+S  I+NVA+GCGHDNEGLF GAAGL+GLGG  LS+++
Sbjct: 250 QVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITN 309

Query: 311 QIKASSFSYCLVDRDSDSSSTLEFNSGR-PGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQ 370
           Q+KA+SFSYCLVDRDS  SS+L+FNS +  G   T PLL N+K  ++ YV ++G SVGG+
Sbjct: 310 QMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGE 369

Query: 371 PLPISSTRFEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPP-AAGISPFD 430
            + +    F++D SG GG+I+D GT +TRL +  Y++L+ AF+KLT NL   ++ IS FD
Sbjct: 370 KVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFD 429

Query: 431 TCYDLSSQSSVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNF 488
           TCYD SS S+V VPTV+F  + GKSL LPA NYLI +D +GT+C AF PT++SLSIIGN 
Sbjct: 430 TCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNV 489

[Close]

BLAST of MC06g0301 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.7e-99
Identity = 192/422 (45.50%), Postives = 266/422 (63.03%), Query Frame = 0


Query: 70  NSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIR 129
           +S ++L L  R    + +Y+++   + AR+ RD  RV ++   L R      P SDS   
Sbjct: 56  SSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI---LRRISGKVIPSSDSRYE 115

Query: 130 PDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQS 189
            +     +VSG  QGSGEYF RIGVG P +  ++V D+GSD+ W+QC+ C     C+KQS
Sbjct: 116 VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCK---LCYKQS 175

Query: 190 DPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGS 249
           DP+FDP+ S SY+ +SC S  C  ++ +GC SG C Y+V YGDGS+T G  A ETL+F +
Sbjct: 176 DPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF-A 235

Query: 250 SNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIK---ASSFSYCLVDRDSDSSS 309
              + NVA+GCGH N G+F+GAAGL+G+GG ++S   Q+      +F YCLV R +DS+ 
Sbjct: 236 KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTG 295

Query: 310 TLEF-NSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGII 369
           +L F     P  +   PL+ N +  S+ YV + GL VGG  +P+    F++  +G GG++
Sbjct: 296 SLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVV 355

Query: 370 VDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLS 429
           +D+GT +TRLP+  Y A +  F   T NLP A+G+S FDTCYDLS   SV VPTVSF  +
Sbjct: 356 MDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFT 415

Query: 430 EGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSN 488
           EG  L LPA N+L+ +D +GTYC AF  + T LSIIGN QQ+GI+VS+D  N  VGF  N
Sbjct: 416 EGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPN 470

[Close]

BLAST of MC06g0301 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.5e-91
Identity = 222/494 (44.94%), Postives = 284/494 (57.49%), Query Frame = 0


Query: 5   ALLFLFLIIFTS-SLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQF 64
           ALLF     F S   FS   S   L P+       +   F   + S+      S+  S+F
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSE------SLLESEF 66

Query: 65  RNNLSQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPG 124
            +     SS S+ L+        S K    L  +RL RD+ RV+S+       L    PG
Sbjct: 67  ESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSI-----ATLAAQIPG 126

Query: 125 SD--SGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDD 184
            +     RP   +  VVSG SQGSGEYF R+GVG PA+  ++V DTGSDI WLQC  C  
Sbjct: 127 RNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC-- 186

Query: 185 SGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQS--GTCLYQVSYGDGSFTTGE 244
              C+ QSDPIFDP  S +Y+++ C S  C+ LD AGC +   TCLYQVSYGDGSFT G+
Sbjct: 187 -RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGD 246

Query: 245 FATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIK---ASSFSYC 304
           F+TETL+F   N +  VA+GCGHDNEGLFVGAAGL+GLG   LS   Q        FSYC
Sbjct: 247 FSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 306

Query: 305 LVDRDSDS--SSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLP-ISSTR 364
           LVDR + S  SS +  N+     +  TPLL N K  ++ YV + G+SVGG  +P ++++ 
Sbjct: 307 LVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASL 366

Query: 365 FEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQS 424
           F++D  G GG+I+DSGT++TRL    Y A++ AF      L  A   S FDTC+DLS+ +
Sbjct: 367 FKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMN 426

Query: 425 SVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSY 484
            V VPTV      G  + LPA NYLI +D+ G +C AF  T   LSIIGN QQQG RV Y
Sbjct: 427 EVKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVY 484

Query: 485 DLVNSLVGFSSNQC 488
           DL +S VGF+   C
Sbjct: 487 DLASSRVGFAPGGC 484

[Close]

BLAST of MC06g0301 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.3e-65
Identity = 164/401 (40.90%), Postives = 214/401 (53.37%), Query Frame = 0


Query: 94  LVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDAVTVPVVSGQSQGSGEYFARIG 153
           L++  + R   R++S+N  L          S SGI       PV +    G GEY   + 
Sbjct: 60  LIKRAIKRGERRMRSINAMLQ---------SSSGIE-----TPVYA----GDGEYLMNVA 119

Query: 154 VGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKS 213
           +G P  SF  + DTGSD+ W QC+ C     CF Q  PIF+P  SSS+S+L C+SQ C+ 
Sbjct: 120 IGTPDSSFSAIMDTGSDLIWTQCEPCT---QCFSQPTPIFNPQDSSSFSTLPCESQYCQD 179

Query: 214 LDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNSISNVAIGCGHDNEGLFVG-AA 273
           L    C +  C Y   YGDGS T G  ATET +F +S S+ N+A GCG DN+G   G  A
Sbjct: 180 LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGA 239

Query: 274 GLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFN---SGRPGNSVTTPLLVNRKF 333
           GLIG+G   LSL SQ+    FSYC+    S S STL      SG P  S +T L+ +   
Sbjct: 240 GLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLN 299

Query: 334 KSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVK 393
            +Y Y+ + G++VGG  L I S+ F++   G GG+I+DSGTT+T LP D Y+A+  AF  
Sbjct: 300 PTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTD 359

Query: 394 LTGNLPPA-AGISPFDTCYDLSSQ-SSVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTY 453
              NLP      S   TC+   S  S+V VP +S Q  +G  L L   N LI   + G  
Sbjct: 360 QI-NLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQF-DGGVLNLGEQNILI-SPAEGVI 419

Query: 454 CLAF-LPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 488
           CLA    +   +SI GN QQQ  +V YDL N  V F   QC
Sbjct: 420 CLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MC06g0301 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.6e-64
Identity = 150/353 (42.49%), Postives = 202/353 (57.22%), Query Frame = 0


Query: 144 GSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPIFDPSSSSSYSS 203
           G GEY   + +G PAQ F  + DTGSD+ W QC+ C     CF QS PIF+P  SSS+S+
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCT---QCFNQSTPIFNPQGSSSFST 150

Query: 204 LSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNSISNVAIGCGHD 263
           L C SQ C++L    C +  C Y   YGDGS T G   TETL+FGS  SI N+  GCG +
Sbjct: 151 LPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSV-SIPNITFGCGEN 210

Query: 264 NEGLFVG-AAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNSGRPGNSVT-- 323
           N+G   G  AGL+G+G   LSL SQ+  + FSYC+    S + S L    G   NSVT  
Sbjct: 211 NQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLL--GSLANSVTAG 270

Query: 324 ---TPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEID-GSGLGGIIVDSGTTITRLP 383
              T L+ + +  ++ Y+ + GLSVG   LPI  + F ++  +G GGII+DSGTT+T   
Sbjct: 271 SPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFV 330

Query: 384 SDVYDALKGAFVKLTGNLPPAAGISP-FDTCYDL-SSQSSVDVPTVSFQLSEGKSLRLPA 443
           ++ Y +++  F+    NLP   G S  FD C+   S  S++ +PT      +G  L LP+
Sbjct: 331 NNAYQSVRQEFISQI-NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHF-DGGDLELPS 390

Query: 444 NNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 488
            NY I   S G  CLA   ++  +SI GN QQQ + V YD  NS+V F+S QC
Sbjct: 391 ENYFI-SPSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MC06g0301 vs. NCBI nr
Match: XP_023531915.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660 bits (1704), Expect = 3.39e-234
Identity = 346/480 (72.08%), Postives = 402/480 (83.75%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           FL + I  S LFS +L+R           T TTA+FDVSASS RAQNALSI P QF ++ 
Sbjct: 17  FLRIAILNSLLFSSSLARVF---------TQTTAVFDVSASSNRAQNALSITPPQFHSHR 76

Query: 68  SQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSG 127
             NSS SLPLH RLA+H  +YKDY SLVRARLARDAARVQSLN+NL+ AL      +   
Sbjct: 77  LSNSSLSLPLHSRLAIHKHNYKDYDSLVRARLARDAARVQSLNRNLNLAL------ARDA 136

Query: 128 IRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFK 187
           +RP+++T PVVSGQSQGSGEYFARI VGQPAQSF+LVPDTGSDITWLQC  C    +C++
Sbjct: 137 VRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSYGNTCYQ 196

Query: 188 QSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSF 247
           Q+DPIF+P+SSSSY  LSCDSQQC+SLDR GCQSGTC YQV YGDGSFTTG+FATETL+F
Sbjct: 197 QTDPIFNPTSSSSYRPLSCDSQQCQSLDRPGCQSGTCAYQVRYGDGSFTTGDFATETLTF 256

Query: 248 GSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSST 307
           G+S SI N+ IGCGHDNEGLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSST
Sbjct: 257 GNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSST 316

Query: 308 LEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVD 367
           LEF+S RP +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVD
Sbjct: 317 LEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVD 376

Query: 368 SGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEG 427
           SGT ITRLP+DVY++L+ AFV+   +L  A+ ISPFDTCY+L+ Q++V VPTV+F+LS+G
Sbjct: 377 SGTFITRLPTDVYESLREAFVQGAQSLTAASAISPFDTCYNLAGQANVQVPTVAFELSKG 436

Query: 428 KSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           K L+LPA NYLIRMD+AGTYCLAFL TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 437 KLLQLPARNYLIRMDTAGTYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 481

[Close]

BLAST of MC06g0301 vs. NCBI nr
Match: KAG7021604.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 657 bits (1695), Expect = 7.93e-233
Identity = 340/475 (71.58%), Postives = 396/475 (83.37%), Query Frame = 0


Query: 13  IFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSS 72
           +F++ LF   L+  L   S       TT +FDVSASS RAQNALSI P QF ++   NSS
Sbjct: 13  LFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQNALSITPPQFHSHRLSNSS 72

Query: 73  FSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDA 132
            SLPLH RLA+H  SYKDYQSLVRARLARDAARVQSLN+NL+ AL G        +RP++
Sbjct: 73  LSLPLHSRLAIHKHSYKDYQSLVRARLARDAARVQSLNRNLNLALAG------DAVRPNS 132

Query: 133 VTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPI 192
           +T PVVSGQSQGSGEYFARI VGQP QSF+LVPDTGSDITWLQC  C    +C++Q+DPI
Sbjct: 133 LTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGNTCYQQTDPI 192

Query: 193 FDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNS 252
           F+P+SSSSY  LSCDSQQC+SL+R GCQSGTC+YQV YGDGSFT G+F TETL+FG+S S
Sbjct: 193 FNPTSSSSYKPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTETLTFGNSKS 252

Query: 253 ISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNS 312
           I N+ IGCGHDNEGLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSSTLEF+S
Sbjct: 253 IPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSSTLEFDS 312

Query: 313 GRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTI 372
             P +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVDSGT I
Sbjct: 313 APPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVDSGTFI 372

Query: 373 TRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRL 432
           TRLP+DVY++L+ AFV+   +L  A  ISPFDTCY+L+ QS+V VPTV+F+LS+GK L+L
Sbjct: 373 TRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFELSKGKLLQL 432

Query: 433 PANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           PANNYLIRMD+AG+YCLAFL TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 433 PANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 481

[Close]

BLAST of MC06g0301 vs. NCBI nr
Match: XP_023007215.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 653 bits (1684), Expect = 3.87e-231
Identity = 341/476 (71.64%), Postives = 398/476 (83.61%), Query Frame = 0


Query: 13  IFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSS 72
           IF++ LF+  L+  L   S     T TT +FDVSASS RAQNALSI P QF ++   NSS
Sbjct: 13  IFSAFLFTAILNSLLFSSSLARVFTETTTVFDVSASSNRAQNALSITPPQFHSHHLSNSS 72

Query: 73  FSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDA 132
            SL LH RLA+H  +YKDY+SLVRARLARDAARVQSLN+NL+ AL G        +RP++
Sbjct: 73  LSLSLHSRLAIHKHNYKDYESLVRARLARDAARVQSLNRNLNLALAG------DAVRPNS 132

Query: 133 VTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPI 192
           +T PVVSGQSQGSGEYFARI VGQPAQSF+LVPDTGSDITWLQC  C    +C+ Q+DPI
Sbjct: 133 LTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSIGNTCYPQTDPI 192

Query: 193 FDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNS 252
           F+P+SSSSY  LSCDSQQC+SL+R GCQSGTC+YQV YGDGSFTTG+FATETL+FG+S S
Sbjct: 193 FNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGDGSFTTGDFATETLTFGNSKS 252

Query: 253 ISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNS 312
           I N+ IGCGHDN+GLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSSTLEF+S
Sbjct: 253 IPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSSTLEFDS 312

Query: 313 GRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTI 372
            RP +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVDSGT I
Sbjct: 313 ARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVDSGTFI 372

Query: 373 TRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRL 432
           TRLP+DVY++L+ AFV+   +L  A  ISPFDTCY+L+ QS+V VPTV+F+LS+G  L+L
Sbjct: 373 TRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQSNVQVPTVAFELSKGNLLQL 432

Query: 433 PANNYLIRMDSAGTYCLAFLP-TTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           PA NYLIRMD+AGTYCLAFL  TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 433 PARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 482

[Close]

BLAST of MC06g0301 vs. NCBI nr
Match: XP_022933467.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata] >KAG6587644.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 652 bits (1683), Expect = 5.29e-231
Identity = 337/475 (70.95%), Postives = 396/475 (83.37%), Query Frame = 0


Query: 13  IFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSS 72
           +F++ LF   L+  L   S       TT +FDVSASS RAQ+ALS+ P QF ++   NSS
Sbjct: 13  LFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQSALSMTPPQFHSHRLSNSS 72

Query: 73  FSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDA 132
            SLPLH RLA+H  SYKDY+SLVRARLARDAARVQSLN+NL+ AL G        +RP++
Sbjct: 73  LSLPLHSRLAIHKHSYKDYESLVRARLARDAARVQSLNRNLNLALAG------DAVRPNS 132

Query: 133 VTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPI 192
           +T PVVSGQSQGSGEYFARI VGQP QSF+LVPDTGSDITWLQC  C    +C++Q+DPI
Sbjct: 133 LTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGNTCYQQTDPI 192

Query: 193 FDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNS 252
           F+P+SSSSY  LSCDSQQC+SL+R GCQSGTC+YQV YGDGSFT G+F TETL+FG+S S
Sbjct: 193 FNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTETLTFGNSKS 252

Query: 253 ISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNS 312
           I N+ IGCGHDNEGLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSSTLEF+S
Sbjct: 253 IPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSSTLEFDS 312

Query: 313 GRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTI 372
             P +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVDSGT I
Sbjct: 313 APPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVDSGTFI 372

Query: 373 TRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRL 432
           TRLP+DVY++L+ AFV+   +L  A  ISPFDTCY+L+ QS+V VPTV+F+LS+GK L+L
Sbjct: 373 TRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFELSKGKLLQL 432

Query: 433 PANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           PANNYLIRMD+AG+YCLAFL TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 433 PANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 481

[Close]

BLAST of MC06g0301 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 640 bits (1650), Expect = 6.83e-226
Identity = 342/485 (70.52%), Postives = 390/485 (80.41%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           F+FL I TS  F    SR L    ++      TAIFDVSAS+K+AQNALSI P  F  + 
Sbjct: 9   FIFLTILTSLQFPSIFSRKLTQSPYS------TAIFDVSASTKQAQNALSIKPKPFETHS 68

Query: 68  SQ-NSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTP---G 127
              NS  SLPLHPRL L+NPSYKDY SLVRARLARDA RVQSLN+NL  +LNGG     G
Sbjct: 69  HHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQKIGAG 128

Query: 128 SDSGIRPDAVTVPVVSGQSQGSG-EYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDS 187
            +     D++T PVVSGQS G+G EYFARIGVGQP QSF+LVPDTGSD+TWLQC+ C   
Sbjct: 129 INGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQPCAAE 188

Query: 188 GSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFAT 247
            +C+KQ DPIFDP SSSSY++LSC+SQQC+ LD+A C S  C YQV+YGDGSFTTGE AT
Sbjct: 189 KACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTTGELAT 248

Query: 248 ETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDS 307
           ETLSFG+SNSI N+ IGCGHDNEGLFVGAAGLIGLGG A+SLSSQ+KASSFSYCLVD DS
Sbjct: 249 ETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCLVDLDS 308

Query: 308 DSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLG 367
           DSSSTLEFN+ RP +S+T+PL+ N +F SYRYV+V G+SVGG PLPISSTRFEID SGLG
Sbjct: 309 DSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEIDESGLG 368

Query: 368 GIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSF 427
           GIIVDSGTTIT+LPSDVY++L+ AFVK T NLPPA GIS FDTCYDLS QSSV+VP ++F
Sbjct: 369 GIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEVPVIAF 428

Query: 428 QLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGF 487
            L    SLRLPA NYLI +DS GTYCLAF  T +SLSIIG+FQQQGIRVSYDL NSLVGF
Sbjct: 429 VLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLANSLVGF 487

[Close]

BLAST of MC06g0301 vs. ExPASy TrEMBL
Match: A0A6J1L2D2 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499769 PE=4 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 1.87e-231
Identity = 341/476 (71.64%), Postives = 398/476 (83.61%), Query Frame = 0


Query: 13  IFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSS 72
           IF++ LF+  L+  L   S     T TT +FDVSASS RAQNALSI P QF ++   NSS
Sbjct: 13  IFSAFLFTAILNSLLFSSSLARVFTETTTVFDVSASSNRAQNALSITPPQFHSHHLSNSS 72

Query: 73  FSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDA 132
            SL LH RLA+H  +YKDY+SLVRARLARDAARVQSLN+NL+ AL G        +RP++
Sbjct: 73  LSLSLHSRLAIHKHNYKDYESLVRARLARDAARVQSLNRNLNLALAG------DAVRPNS 132

Query: 133 VTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPI 192
           +T PVVSGQSQGSGEYFARI VGQPAQSF+LVPDTGSDITWLQC  C    +C+ Q+DPI
Sbjct: 133 LTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSIGNTCYPQTDPI 192

Query: 193 FDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNS 252
           F+P+SSSSY  LSCDSQQC+SL+R GCQSGTC+YQV YGDGSFTTG+FATETL+FG+S S
Sbjct: 193 FNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGDGSFTTGDFATETLTFGNSKS 252

Query: 253 ISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNS 312
           I N+ IGCGHDN+GLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSSTLEF+S
Sbjct: 253 IPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSSTLEFDS 312

Query: 313 GRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTI 372
            RP +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVDSGT I
Sbjct: 313 ARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVDSGTFI 372

Query: 373 TRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRL 432
           TRLP+DVY++L+ AFV+   +L  A  ISPFDTCY+L+ QS+V VPTV+F+LS+G  L+L
Sbjct: 373 TRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQSNVQVPTVAFELSKGNLLQL 432

Query: 433 PANNYLIRMDSAGTYCLAFLP-TTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           PA NYLIRMD+AGTYCLAFL  TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 433 PARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 482

[Close]

BLAST of MC06g0301 vs. ExPASy TrEMBL
Match: A0A6J1EZU8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111440870 PE=4 SV=1)

HSP 1 Score: 652 bits (1683), Expect = 2.56e-231
Identity = 337/475 (70.95%), Postives = 396/475 (83.37%), Query Frame = 0


Query: 13  IFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNLSQNSS 72
           +F++ LF   L+  L   S       TT +FDVSASS RAQ+ALS+ P QF ++   NSS
Sbjct: 13  LFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQSALSMTPPQFHSHRLSNSS 72

Query: 73  FSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIRPDA 132
            SLPLH RLA+H  SYKDY+SLVRARLARDAARVQSLN+NL+ AL G        +RP++
Sbjct: 73  LSLPLHSRLAIHKHSYKDYESLVRARLARDAARVQSLNRNLNLALAG------DAVRPNS 132

Query: 133 VTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQSDPI 192
           +T PVVSGQSQGSGEYFARI VGQP QSF+LVPDTGSDITWLQC  C    +C++Q+DPI
Sbjct: 133 LTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGNTCYQQTDPI 192

Query: 193 FDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGSSNS 252
           F+P+SSSSY  LSCDSQQC+SL+R GCQSGTC+YQV YGDGSFT G+F TETL+FG+S S
Sbjct: 193 FNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTETLTFGNSKS 252

Query: 253 ISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDSDSSSTLEFNS 312
           I N+ IGCGHDNEGLFVGAAGLIGLGG ALSLSSQ+KASSFSYCLVDRDSDSSSTLEF+S
Sbjct: 253 IPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSDSSSTLEFDS 312

Query: 313 GRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGIIVDSGTTI 372
             P +S+TTPLL N +  SYRYV+VTG+SVGG+ L ISSTRFEIDGSG+GGIIVDSGT I
Sbjct: 313 APPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGGIIVDSGTFI 372

Query: 373 TRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRL 432
           TRLP+DVY++L+ AFV+   +L  A  ISPFDTCY+L+ QS+V VPTV+F+LS+GK L+L
Sbjct: 373 TRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFELSKGKLLQL 432

Query: 433 PANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSNQC 487
           PANNYLIRMD+AG+YCLAFL TT+SLSIIG+FQQQG+RVSYDLVNSLVGFSSN+C
Sbjct: 433 PANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFSSNKC 481

[Close]

BLAST of MC06g0301 vs. ExPASy TrEMBL
Match: A0A0A0LPJ3 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=4 SV=1)

HSP 1 Score: 637 bits (1644), Expect = 2.70e-225
Identity = 338/485 (69.69%), Postives = 395/485 (81.44%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           FLFL IFTS  F   LSR L   S++      T+IFDVSAS+ +A +ALSI P   +N+ 
Sbjct: 9   FLFLTIFTSLQFPSILSRKLTPSSYS------TSIFDVSASTNQALDALSIKPKPLQNHS 68

Query: 68  S-QNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDS 127
              NS FSLPL+PRLALHNPSYKDY +LVRARL RDAARVQ LN+NL R+LNGGT   +S
Sbjct: 69  HLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFGES 128

Query: 128 ---GIRPDAVTVPVVSGQSQGSG-EYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDS 187
               +  D++T PVVSGQS+GSG EY A+IGVGQP + F+LVPDTGSD+TWLQC+ C   
Sbjct: 129 INESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCASE 188

Query: 188 GSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFAT 247
            +C+KQ DPIFDP SSSSYS LSC+SQQCK LD+A C S TC+YQV YGDGSFTTGE AT
Sbjct: 189 NTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGELAT 248

Query: 248 ETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDRDS 307
           ETLSFG+SNSI N+ IGCGHDNEGLF G AGLIGLGG A+SLSSQ+KASSFSYCLV+ DS
Sbjct: 249 ETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNLDS 308

Query: 308 DSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLG 367
           DSSSTLEFNS  P +S+T+PL+ N +F SYRYV+V G+SVGG+ LPIS TRFEID SGLG
Sbjct: 309 DSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESGLG 368

Query: 368 GIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSF 427
           GIIVDSGT I+RLPSDVY++L+ AFVKLT +L PA GIS FDTCY+ S QS+V+VPT++F
Sbjct: 369 GIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTIAF 428

Query: 428 QLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGF 487
            LSEG SLRLPA NYLI +D+AGTYCLAF+ T +SLSIIG+FQQQGIRVSYDL NSLVGF
Sbjct: 429 VLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGF 487

[Close]

BLAST of MC06g0301 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 1.80e-223
Identity = 340/488 (69.67%), Postives = 395/488 (80.94%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           FLFL IFTS  FS  LSR L    ++      T+IFDV AS+ +A NALSI P   + + 
Sbjct: 9   FLFLTIFTSLQFSSILSRKLTQSPYS------TSIFDVLASTNQALNALSIKPKHLQTHS 68

Query: 68  S-QNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGG------ 127
              NSS SLPL+PRL+LHNPSYKDY SLVRARLARDAARVQ LN+NL  +LNGG      
Sbjct: 69  HLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFGEV 128

Query: 128 TPGSDSGIRPDAVTVPVVSGQSQGSG-EYFARIGVGQPAQSFFLVPDTGSDITWLQCKSC 187
           T GS  G   D++T PVVSGQS+GSG EY A++GVGQP + F+LVPDTGSD+TWLQC+ C
Sbjct: 129 TNGSLIG---DSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 188

Query: 188 DDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGE 247
               +C+KQ DPIFDP SSSSY+ LSC+SQQC  LDR  C SGTC+YQV YGDGSFTTGE
Sbjct: 189 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 248

Query: 248 FATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVD 307
            ATETLSFG+SNSI N+ IGCGHDNEGLF G AGLIGLGG A+SLSSQ+KASSFSYCLV+
Sbjct: 249 LATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 308

Query: 308 RDSDSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGS 367
            DSDSSSTLEFNS  P +S+T+PL+ N +F SYRYV+V G+SVGG+ LPISSTRFEID S
Sbjct: 309 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDES 368

Query: 368 GLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPT 427
           GLGGIIVDSGT I+RLPSDVY++L+ AFVKLT +L PA GIS FDTCY+LSSQS+V+VPT
Sbjct: 369 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPT 428

Query: 428 VSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSL 487
           ++F LS G SLRLPA NYLIR+D+AGTYCLAF+ T +SLSIIG+FQQQGIRVSYDL NSL
Sbjct: 429 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 487

[Close]

BLAST of MC06g0301 vs. ExPASy TrEMBL
Match: A0A5D3DYG2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00370 PE=4 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 5.95e-222
Identity = 338/488 (69.26%), Postives = 392/488 (80.33%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           FLFL IFTS  FS  LSR L    ++      T+IFDV AS+ +A NALSI P   + + 
Sbjct: 9   FLFLTIFTSLQFSSILSRKLTQSPYS------TSIFDVLASTNQALNALSIKPKHLQTHS 68

Query: 68  S-QNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGG------ 127
              NSS SLPL+PRL+LHNPSYKDY SLVRARLARDAARVQ LN+NL  +LNGG      
Sbjct: 69  HLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFGEV 128

Query: 128 TPGSDSGIRPDAVTVPVVSGQSQGSG-EYFARIGVGQPAQSFFLVPDTGSDITWLQCKSC 187
           T GS  G   D++T PVVSGQS+GSG EY A++GVGQP + F+LVPDTGSD+TWLQC+ C
Sbjct: 129 TNGSLIG---DSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 188

Query: 188 DDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGE 247
               +C+KQ DPIFDP SSSSY+ LSC+SQQC  LDR  C SGTC+YQV YGDGSFTTGE
Sbjct: 189 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 248

Query: 248 FATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVD 307
            ATE LSFG+SNSI N+ IGCGHDNEGLF G AGLIGLGG A+SLSSQ+KASSFSYCLV+
Sbjct: 249 LATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 308

Query: 308 RDSDSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGS 367
            DSDSSSTLEFNS  P +S+T+PL+ N +F SYRYV+V G+SVGG  LPISSTRFEID S
Sbjct: 309 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDES 368

Query: 368 GLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPT 427
           GLGGIIVDSGT I+RLPSDVY++L+ AFVKLT +L PA GIS FDTCY+LS QS+V+VPT
Sbjct: 369 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPT 428

Query: 428 VSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSL 487
           ++F LS G SLRLPA NYLIR+D+AGTYCLAF+ T +SLSIIG+FQQQGIRVSYDL NSL
Sbjct: 429 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 487

[Close]

BLAST of MC06g0301 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 1.6e-131
Identity = 251/487 (51.54%), Postives = 341/487 (70.02%), Query Frame = 0


Query: 8   FLFLIIFTSSLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 67
           F F I F +S  S   SR L       T T TT+I +V+ S  R +   S   +Q     
Sbjct: 7   FFFFIFFLTS-HSSVFSRIL-----PETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQT 66

Query: 68  -SQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDS 127
            S +SSFSL LH R+++    + DY+SL  ARL RD ARV+SL   L  A+N  +     
Sbjct: 67  HSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLK 126

Query: 128 GI------RPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCD 187
            I          +  P++SG +QGSGEYF R+G+G+PA+  ++V DTGSD+ WLQC  C 
Sbjct: 127 PISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCA 186

Query: 188 DSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEF 247
           D   C+ Q++PIF+PSSSSSY  LSCD+ QC +L+ + C++ TCLY+VSYGDGS+T G+F
Sbjct: 187 D---CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDF 246

Query: 248 ATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIKASSFSYCLVDR 307
           ATETL+ GS+  + NVA+GCGH NEGLFVGAAGL+GLGG  L+L SQ+  +SFSYCLVDR
Sbjct: 247 ATETLTIGST-LVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDR 306

Query: 308 DSDSSSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSG 367
           DSDS+ST++F +    ++V  PLL N +  ++ Y+ +TG+SVGG+ L I  + FE+D SG
Sbjct: 307 DSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESG 366

Query: 368 LGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTV 427
            GGII+DSGT +TRL +++Y++L+ +FVK T +L  AAG++ FDTCY+LS++++V+VPTV
Sbjct: 367 SGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTV 426

Query: 428 SFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLV 487
           +F    GK L LPA NY+I +DS GT+CLAF PT +SL+IIGN QQQG RV++DL NSL+
Sbjct: 427 AFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLI 483

[Close]

BLAST of MC06g0301 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.4e-127
Identity = 249/503 (49.50%), Postives = 340/503 (67.59%), Query Frame = 0


Query: 11  LIIFTSSLFSPTL---SRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQFRNNL 70
           L + T SLF  T    SR+L  P  T+       + DV +S ++ Q  LS+ P+  R++L
Sbjct: 10  LAVVTLSLFLTTTDASSRSLSTPPKTN-------VLDVVSSLQQTQTILSLDPT--RSSL 69

Query: 71  --------------SQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNL 130
                         + +S  SL LH R       +KDY+SL  +RL RD++RV  +   +
Sbjct: 70  TTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 129

Query: 131 HRALNG-------GTPGSDSGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPD 190
             A+ G            D+  + + +T PVVSG SQGSGEYF+RIGVG PA+  +LV D
Sbjct: 130 RFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLD 189

Query: 191 TGSDITWLQCKSCDDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLY 250
           TGSD+ W+QC+ C D   C++QSDP+F+P+SSS+Y SL+C + QC  L+ + C+S  CLY
Sbjct: 190 TGSDVNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLY 249

Query: 251 QVSYGDGSFTTGEFATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSS 310
           QVSYGDGSFT GE AT+T++FG+S  I+NVA+GCGHDNEGLF GAAGL+GLGG  LS+++
Sbjct: 250 QVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITN 309

Query: 311 QIKASSFSYCLVDRDSDSSSTLEFNSGR-PGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQ 370
           Q+KA+SFSYCLVDRDS  SS+L+FNS +  G   T PLL N+K  ++ YV ++G SVGG+
Sbjct: 310 QMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGE 369

Query: 371 PLPISSTRFEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPP-AAGISPFD 430
            + +    F++D SG GG+I+D GT +TRL +  Y++L+ AF+KLT NL   ++ IS FD
Sbjct: 370 KVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFD 429

Query: 431 TCYDLSSQSSVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNF 488
           TCYD SS S+V VPTV+F  + GKSL LPA NYLI +D +GT+C AF PT++SLSIIGN 
Sbjct: 430 TCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNV 489

[Close]

BLAST of MC06g0301 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.5e-100
Identity = 192/422 (45.50%), Postives = 266/422 (63.03%), Query Frame = 0


Query: 70  NSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPGSDSGIR 129
           +S ++L L  R    + +Y+++   + AR+ RD  RV ++   L R      P SDS   
Sbjct: 56  SSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI---LRRISGKVIPSSDSRYE 115

Query: 130 PDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDDSGSCFKQS 189
            +     +VSG  QGSGEYF RIGVG P +  ++V D+GSD+ W+QC+ C     C+KQS
Sbjct: 116 VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCK---LCYKQS 175

Query: 190 DPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQSGTCLYQVSYGDGSFTTGEFATETLSFGS 249
           DP+FDP+ S SY+ +SC S  C  ++ +GC SG C Y+V YGDGS+T G  A ETL+F +
Sbjct: 176 DPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF-A 235

Query: 250 SNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIK---ASSFSYCLVDRDSDSSS 309
              + NVA+GCGH N G+F+GAAGL+G+GG ++S   Q+      +F YCLV R +DS+ 
Sbjct: 236 KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTG 295

Query: 310 TLEF-NSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLPISSTRFEIDGSGLGGII 369
           +L F     P  +   PL+ N +  S+ YV + GL VGG  +P+    F++  +G GG++
Sbjct: 296 SLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVV 355

Query: 370 VDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQSSVDVPTVSFQLS 429
           +D+GT +TRLP+  Y A +  F   T NLP A+G+S FDTCYDLS   SV VPTVSF  +
Sbjct: 356 MDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFT 415

Query: 430 EGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSYDLVNSLVGFSSN 488
           EG  L LPA N+L+ +D +GTYC AF  + T LSIIGN QQ+GI+VS+D  N  VGF  N
Sbjct: 416 EGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPN 470

[Close]

BLAST of MC06g0301 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 339.3 bits (869), Expect = 5.0e-93
Identity = 227/508 (44.69%), Postives = 305/508 (60.04%), Query Frame = 0


Query: 1   IMNMALLFLFLII-FTSSLFS--PTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALS 60
           ++N     +F ++ FTSS  S   TL    L  S T +   + ++ D S S   +  +LS
Sbjct: 5   VLNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLS--ESTTSLS 64

Query: 61  IHPSQFRNNLSQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQ----NL 120
           +H S    ++   SSFS          + S  D   L   RL RD+ RV+S+      + 
Sbjct: 65  VHLS----HVDALSSFS----------DASPAD---LFNLRLQRDSLRVKSITSLAAVST 124

Query: 121 HRALNGGTPGSDSGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITW 180
            R     TP +  G      +  V+SG SQGSGEYF R+GVG PA + ++V DTGSD+ W
Sbjct: 125 GRNATKRTPRTAGGF-----SGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVW 184

Query: 181 LQCKSCDDSGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAG-C---QSGTCLYQVS 240
           LQC  C    +C+ Q+D IFDP  S +++++ C S+ C+ LD +  C   +S TCLYQVS
Sbjct: 185 LQCSPCK---ACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVS 244

Query: 241 YGDGSFTTGEFATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIK 300
           YGDGSFT G+F+TETL+F  +  + +V +GCGHDNEGLFVGAAGL+GLG   LS  SQ K
Sbjct: 245 YGDGSFTEGDFSTETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTK 304

Query: 301 ---ASSFSYCLVDRDSDSS-----STLEF-NSGRPGNSVTTPLLVNRKFKSYRYVEVTGL 360
                 FSYCLVDR S  S     ST+ F N+  P  SV TPLL N K  ++ Y+++ G+
Sbjct: 305 NRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGI 364

Query: 361 SVGGQPLP-ISSTRFEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAG 420
           SVGG  +P +S ++F++D +G GG+I+DSGT++TRL    Y AL+ AF      L  A  
Sbjct: 365 SVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPS 424

Query: 421 ISPFDTCYDLSSQSSVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLS 480
            S FDTC+DLS  ++V VPTV F    G+ + LPA+NYLI +++ G +C AF  T  SLS
Sbjct: 425 YSLFDTCFDLSGMTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLS 483

Query: 481 IIGNFQQQGIRVSYDLVNSLVGFSSNQC 488
           IIGN QQQG RV+YDLV S VGF S  C
Sbjct: 485 IIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of MC06g0301 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 222/494 (44.94%), Postives = 284/494 (57.49%), Query Frame = 0


Query: 5   ALLFLFLIIFTS-SLFSPTLSRTLLGPSHTHTHTHTTAIFDVSASSKRAQNALSIHPSQF 64
           ALLF     F S   FS   S   L P+       +   F   + S+      S+  S+F
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSE------SLLESEF 66

Query: 65  RNNLSQNSSFSLPLHPRLALHNPSYKDYQSLVRARLARDAARVQSLNQNLHRALNGGTPG 124
            +     SS S+ L+        S K    L  +RL RD+ RV+S+       L    PG
Sbjct: 67  ESGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSI-----ATLAAQIPG 126

Query: 125 SD--SGIRPDAVTVPVVSGQSQGSGEYFARIGVGQPAQSFFLVPDTGSDITWLQCKSCDD 184
            +     RP   +  VVSG SQGSGEYF R+GVG PA+  ++V DTGSDI WLQC  C  
Sbjct: 127 RNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC-- 186

Query: 185 SGSCFKQSDPIFDPSSSSSYSSLSCDSQQCKSLDRAGCQS--GTCLYQVSYGDGSFTTGE 244
              C+ QSDPIFDP  S +Y+++ C S  C+ LD AGC +   TCLYQVSYGDGSFT G+
Sbjct: 187 -RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGD 246

Query: 245 FATETLSFGSSNSISNVAIGCGHDNEGLFVGAAGLIGLGGAALSLSSQIK---ASSFSYC 304
           F+TETL+F   N +  VA+GCGHDNEGLFVGAAGL+GLG   LS   Q        FSYC
Sbjct: 247 FSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 306

Query: 305 LVDRDSDS--SSTLEFNSGRPGNSVTTPLLVNRKFKSYRYVEVTGLSVGGQPLP-ISSTR 364
           LVDR + S  SS +  N+     +  TPLL N K  ++ YV + G+SVGG  +P ++++ 
Sbjct: 307 LVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASL 366

Query: 365 FEIDGSGLGGIIVDSGTTITRLPSDVYDALKGAFVKLTGNLPPAAGISPFDTCYDLSSQS 424
           F++D  G GG+I+DSGT++TRL    Y A++ AF      L  A   S FDTC+DLS+ +
Sbjct: 367 FKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMN 426

Query: 425 SVDVPTVSFQLSEGKSLRLPANNYLIRMDSAGTYCLAFLPTTTSLSIIGNFQQQGIRVSY 484
            V VPTV      G  + LPA NYLI +D+ G +C AF  T   LSIIGN QQQG RV Y
Sbjct: 427 EVKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVY 484

Query: 485 DLVNSLVGFSSNQC 488
           DL +S VGF+   C
Sbjct: 487 DLASSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of MC06g0301 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	3.3e-126	49.50	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	7.7e-99	45.50	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	3.5e-91	44.94	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	7.3e-65	40.90	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	1.6e-64	42.49	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of MC06g0301 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531915.1	3.39e-234	72.08	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7021604.1	7.93e-233	71.58	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_023007215.1	3.87e-231	71.64	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]
XP_022933467.1	5.29e-231	70.95	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata] >KAG6587644....	[more]
XP_038878113.1	6.83e-226	70.52	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida]	[more]

BLAST of MC06g0301 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L2D2	1.87e-231	71.64	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EZU8	2.56e-231	70.95	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LPJ3	2.70e-225	69.69	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=...	[more]
A0A5A7UQC2	1.80e-223	69.67	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3DYG2	5.95e-222	69.26	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]

BLAST of MC06g0301 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25510.1	1.6e-131	51.54	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	2.4e-127	49.50	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	5.5e-100	45.50	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	5.0e-93	44.69	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.5e-92	44.94	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 334..483 e-value: 4.9E-37 score: 127.2
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 148..310 e-value: 2.5E-47 score: 161.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 312..487 e-value: 6.4E-51 score: 174.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 123..310 e-value: 6.2E-51 score: 175.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 141..487
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 23..487
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 23..487
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 364..375
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 148..483 score: 46.908504

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0301.1	MC06g0301.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016020	membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity