MC06g0279 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0279
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	EF hand calcium-binding protein family
Location	MC06: 2278112 .. 2278525 (+)
RNA-Seq Expression	MC06g0279
Synteny	MC06g0279

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATG

mRNA sequence

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATG

Coding sequence (CDS)

AATCAATTACGCGACATATTTGCACGATTCGACATGGATTCCGACGGAAGCCTGACGATTCTTGAGCTGGCGGCGTTGCTCCGGTCGCTAGGTCTAAAGCCCTCCGGCGACCAAATCCACGTTCTCGTCGCGAACATGGATGCGAACGGGAATGGATCTGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCGGATTTCAACGAGGAGGTAATGGTAAATCAGACGCAGCTTCTGGAGGTTTTCCGTTCCTTCGATCGAGACGGGAATGGATATATAACCGCGGCGGAGCTGGCGGGATCGATGGCGAAGATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGACGGAGATGGCGTAATTAGCTTCAACGAGTTTGCATCTGTAATG

Protein sequence

NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFNEFASVM

Homology

[Close]

BLAST of MC06g0279 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 7.6e-60
Identity = 112/137 (81.75%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFDELV 
Sbjct: 7   QLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVG 66

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEA
Sbjct: 67  TILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEA 126

Query: 122 DTDGDGVISFNEFASVM 139
           DT+GDGVISF EFAS+M
Sbjct: 127 DTNGDGVISFGEFASIM 143

[Close]

BLAST of MC06g0279 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 3.5e-57
Identity = 110/137 (80.29%), Postives = 123/137 (89.78%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFDELV 
Sbjct: 12  QLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVV 71

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
           AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EA
Sbjct: 72  AILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEA 131

Query: 122 DTDGDGVISFNEFASVM 139
           D++GDGVISFNEF+ +M
Sbjct: 132 DSNGDGVISFNEFSHIM 148

[Close]

BLAST of MC06g0279 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.4e-50
Identity = 99/138 (71.74%), Postives = 119/138 (86.23%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QLR++F RFDM+ DGSLT LELAALLRSLGL+P+GD++H L+A MDANGNGSVEFDEL  
Sbjct: 25  QLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDELAA 84

Query: 62  AIMPDFNEEV-MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 121
           AI P    +  +V+Q QLLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT MM++
Sbjct: 85  AIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRMMRD 144

Query: 122 ADTDGDGVISFNEFASVM 139
           ADTDGDGVISF EFA+VM
Sbjct: 145 ADTDGDGVISFKEFAAVM 162

[Close]

BLAST of MC06g0279 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 7.6e-44
Identity = 90/142 (63.38%), Postives = 114/142 (80.28%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKP-SGDQIHVLVANMDANGNGSVEFDELV 61
           QL +IF RFD+D DGSLT LELAALLRSLGL+P +GD+IH L+A +DA+GNG+VEFDEL 
Sbjct: 95  QLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEFDELA 154

Query: 62  TA----IMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTE 121
           ++    I+      V V+Q +L E FR+FDRDGNG+I+AAELA SMA+MG P+ Y ELT+
Sbjct: 155 SSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAELTD 214

Query: 122 MMKEADTDGDGVISFNEFASVM 139
           MM+EADTDGDG+ISF EF ++M
Sbjct: 215 MMREADTDGDGLISFEEFTAIM 236

[Close]

BLAST of MC06g0279 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 7.6e-36
Identity = 80/137 (58.39%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V 
Sbjct: 20  ELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVA 79

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEA
Sbjct: 80  LVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEA 139

Query: 122 DTDGDGVISFNEFASVM 139
           D DGDG I F EF   +
Sbjct: 140 DRDGDGCIDFQEFVQAI 155

[Close]

BLAST of MC06g0279 vs. NCBI nr
Match: XP_022135250.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 270 bits (690), Expect = 8.72e-91
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. NCBI nr
Match: XP_004138337.2 (probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 268 bits (684), Expect = 7.17e-90
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. NCBI nr
Match: XP_038880166.1 (probable calcium-binding protein CML15 [Benincasa hispida])

HSP 1 Score: 268 bits (684), Expect = 7.17e-90
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. NCBI nr
Match: XP_022921807.1 (probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266 bits (681), Expect = 2.06e-89
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. NCBI nr
Match: XP_008453344.1 (PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa] >TYK28384.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa])

HSP 1 Score: 266 bits (680), Expect = 2.92e-89
Identity = 135/138 (97.83%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. ExPASy TrEMBL
Match: A0A6J1C0M6 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111007259 PE=4 SV=1)

HSP 1 Score: 270 bits (690), Expect = 4.22e-91
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. ExPASy TrEMBL
Match: A0A0A0LUS7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1)

HSP 1 Score: 268 bits (684), Expect = 3.47e-90
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. ExPASy TrEMBL
Match: A0A6J1E1G5 (probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC111429950 PE=4 SV=1)

HSP 1 Score: 266 bits (681), Expect = 9.95e-90
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMDANGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAI PDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIRPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. ExPASy TrEMBL
Match: A0A5D3DXV7 (Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00110 PE=4 SV=1)

HSP 1 Score: 266 bits (680), Expect = 1.41e-89
Identity = 135/138 (97.83%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. ExPASy TrEMBL
Match: A0A1S3BX63 (probable calcium-binding protein CML15 OS=Cucumis melo OX=3656 GN=LOC103494094 PE=4 SV=1)

HSP 1 Score: 266 bits (680), Expect = 1.41e-89
Identity = 135/138 (97.83%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELV
Sbjct: 10  NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELV 69

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
           TAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE
Sbjct: 70  TAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 129

Query: 121 ADTDGDGVISFNEFASVM 138
           ADTDGDGVISFNEFASVM
Sbjct: 130 ADTDGDGVISFNEFASVM 147

[Close]

BLAST of MC06g0279 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 231.1 bits (588), Expect = 5.4e-61
Identity = 112/137 (81.75%), Postives = 129/137 (94.16%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVL+A+MD+NGNG VEFDELV 
Sbjct: 7   QLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVG 66

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEA
Sbjct: 67  TILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEA 126

Query: 122 DTDGDGVISFNEFASVM 139
           DT+GDGVISF EFAS+M
Sbjct: 127 DTNGDGVISFGEFASIM 143

[Close]

BLAST of MC06g0279 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 222.2 bits (565), Expect = 2.5e-58
Identity = 110/137 (80.29%), Postives = 123/137 (89.78%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +L+  +D NGNGSVEFDELV 
Sbjct: 12  QLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVV 71

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
           AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EA
Sbjct: 72  AILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEA 131

Query: 122 DTDGDGVISFNEFASVM 139
           D++GDGVISFNEF+ +M
Sbjct: 132 DSNGDGVISFNEFSHIM 148

[Close]

BLAST of MC06g0279 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 151.4 bits (381), Expect = 5.4e-37
Identity = 80/137 (58.39%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 2   QLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVT 61
           +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V 
Sbjct: 20  ELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVA 79

Query: 62  AIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEA 121
            + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEA
Sbjct: 80  LVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEA 139

Query: 122 DTDGDGVISFNEFASVM 139
           D DGDG I F EF   +
Sbjct: 140 DRDGDGCIDFQEFVQAI 155

[Close]

BLAST of MC06g0279 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 146.4 bits (368), Expect = 1.7e-35
Identity = 77/142 (54.23%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           N+LR+IF  FD + DGSLT LEL +LLR+LG+KPS DQ   L+   D   NG VEF E V
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFPEFV 74

Query: 61  TAIMPDF----NEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTE 120
             + P+            + QLL +FR FD DGNG+ITAAELA SMAK+G  LT  ELT 
Sbjct: 75  ALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAELTG 134

Query: 121 MMKEADTDGDGVISFNEFASVM 139
           M+KEAD+DGDG I+F EFA  +
Sbjct: 135 MIKEADSDGDGRINFQEFAKAI 156

[Close]

BLAST of MC06g0279 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 114.0 bits (284), Expect = 9.6e-26
Identity = 55/138 (39.86%), Postives = 88/138 (63.77%), Query Frame = 0


Query: 1   NQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELV 60
           ++ ++ F+ FD D DG +T  EL  ++RSLG  P+  ++  ++  +DA+GNG+++F E +
Sbjct: 11  SEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL 70

Query: 61  TAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKE 120
             +     +    ++ +L E FR FD+D NG+I+AAEL   M  +G+ LT  E+ EM+KE
Sbjct: 71  NLMARKMKD--TDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIKE 130

Query: 121 ADTDGDGVISFNEFASVM 139
           AD DGDG I++ EF  VM
Sbjct: 131 ADVDGDGQINYEEFVKVM 146

The following BLAST results are available for this feature:

BLAST of MC06g0279 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ75	7.6e-60	81.75	Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ...	[more]
Q9LI84	3.5e-57	80.29	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]
Q6L5F4	1.4e-50	71.74	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	7.6e-44	63.38	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9M8U1	7.6e-36	58.39	Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ...	[more]

BLAST of MC06g0279 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135250.1	8.72e-91	100.00	probable calcium-binding protein CML15 [Momordica charantia]	[more]
XP_004138337.2	7.17e-90	98.55	probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probabl...	[more]
XP_038880166.1	7.17e-90	98.55	probable calcium-binding protein CML15 [Benincasa hispida]	[more]
XP_022921807.1	2.06e-89	98.55	probable calcium-binding protein CML15 [Cucurbita moschata] >XP_023516651.1 prob...	[more]
XP_008453344.1	2.92e-89	97.83	PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 p...	[more]

BLAST of MC06g0279 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0M6	4.22e-91	100.00	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0LUS7	3.47e-90	98.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1	[more]
A0A6J1E1G5	9.95e-90	98.55	probable calcium-binding protein CML15 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]
A0A5D3DXV7	1.41e-89	97.83	Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BX63	1.41e-89	97.83	probable calcium-binding protein CML15 OS=Cucumis melo OX=3656 GN=LOC103494094 P...	[more]

BLAST of MC06g0279 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18530.1	5.4e-61	81.75	EF hand calcium-binding protein family	[more]
AT3G25600.1	2.5e-58	80.29	Calcium-binding EF-hand family protein	[more]
AT3G03000.1	5.4e-37	58.39	EF hand calcium-binding protein family	[more]
AT1G32250.1	1.7e-35	54.23	Calcium-binding EF-hand family protein	[more]
AT2G41110.1	9.6e-26	39.86	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 77..105 e-value: 5.7E-4 score: 29.2 coord: 2..30 e-value: 0.046 score: 22.9 coord: 38..66 e-value: 3.1 score: 13.8 coord: 113..138 e-value: 0.01 score: 25.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 76..138 e-value: 3.9E-15 score: 56.0 coord: 2..63 e-value: 7.5E-11 score: 42.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 73..108 score: 14.039183
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 34..69 score: 9.018011
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..33 score: 12.951263
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..138 score: 14.290241
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 77..138 e-value: 1.57069E-18 score: 72.1953
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 2..64 e-value: 1.39576E-12 score: 56.7873
None	No IPR available	GENE3D	1.10.238.10		coord: 69..138 e-value: 1.1E-22 score: 82.1
None	No IPR available	GENE3D	1.10.238.10		coord: 1..68 e-value: 4.6E-17 score: 64.6
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..138
None	No IPR available	PANTHER	PTHR23050:SF351	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 1..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 47..59
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 86..98
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 11..23
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 2..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0279.1	MC06g0279.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity