MC06g0251 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0251 vs. ExPASy Swiss-Prot
Match: Q05549 (ATP-dependent helicase HRQ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRQ1 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.9e-130
Identity = 283/812 (34.85%), Postives = 435/812 (53.57%), Query Frame = 0

Query: 431  MVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIE 490
            M+E LK+      QI H   I +R A Y  +  EL+  V   ++    E  YSHQA +I 
Sbjct: 244  MIEKLKSTEFYASQIKHCFTIPSRTAKYKGLCFELAPEVYQGME---HENFYSHQADAIN 303

Query: 491  ASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMK 550
            +   G++V + T TSSGKSL Y L  ++ + ++  S  +Y+FPTKALAQDQ R+  V++ 
Sbjct: 304  SLHQGENVIITTSTSSGKSLIYQLAAIDLLLKDPESTFMYIFPTKALAQDQKRAFKVILS 363

Query: 551  GFSD--NLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFV 610
               +  N  +  YDGDT   +R  +R NAR++ TNPDM+H SILP H  +   L +L+ V
Sbjct: 364  KIPELKNAVVDTYDGDTEPEERAYIRKNARVIFTNPDMIHTSILPNHANWRHFLYHLKLV 423

Query: 611  LIDEAHTYKGAFGCHTALILRRLRRICSHVY-GSDPSFIFCTATSANPREHCMELGNLSS 670
            ++DE H YKG FG H AL++RRL R+C   Y  S   FI C+AT  +P +H  ++  ++ 
Sbjct: 424  VVDELHIYKGLFGSHVALVMRRLLRLCHCFYENSGLQFISCSATLKSPVQHMKDMFGINE 483

Query: 671  LELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFRNPRQQLSIPIMDIAR 730
            + LI  DGSP+  K  ++WNP   L  ++R         K  NF          I + A+
Sbjct: 484  VTLIHEDGSPTGAKHLVVWNP-PILPQHER---------KRENF----------IRESAK 543

Query: 731  LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERA-PHLVQSVCAYRAGYTAEDRRRI 790
            +  +++ + +R IAFC  R++CEL++   R I  E     LV  V +YR GY+A DRR+I
Sbjct: 544  ILVQLILNNVRTIAFCYVRRVCELLMKEVRNIFIETGREDLVTEVMSYRGGYSASDRRKI 603

Query: 791  ESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRRERTSLSVY 850
            E + F GNL  V +TNALELGID+G +DA L  GFP S+A+  QQ+GRAGRR   SL++ 
Sbjct: 604  EREMFHGNLKAVISTNALELGIDIGGLDAVLMCGFPLSMANFHQQSGRAGRRNNDSLTLV 663

Query: 851  VAFDGPLDQYFMKNPEKLFG----SPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQN 910
            VA D P+DQ+++ +PE L         +   +D  N  +LE H+ CAA E PI+   D+ 
Sbjct: 664  VASDSPVDQHYVAHPESLLEVNNFESYQDLVLDFNNILILEGHIQCAAFELPINFERDKQ 723

Query: 911  FF-GSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETERYRVVD 970
            +F  S L        +      D    S++ +         PS+ VS+R  E +++ VVD
Sbjct: 724  YFTESHLRKICVERLHHNQ---DGYHASNRFL-------PWPSKCVSLRGGEEDQFAVVD 783

Query: 971  --QRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYTKTR 1030
                +N +IEEIE S+  F +Y+G +++HQG  YLVK  N     A  +  D+ + T  R
Sbjct: 784  ITNGRNIIIEEIEASRTSFTLYDGGIFIHQGYPYLVKEFNPDERYAKVQRVDVDWVTNQR 843

Query: 1031 DYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVDLSL 1090
            D+TD+      +    R  ++P           + T   FGF+++ K  K+I D ++   
Sbjct: 844  DFTDVDPQEIELIRSLRNSDVP-----VYFGKIKTTIIVFGFFKVDK-YKRIIDAIETHN 903

Query: 1091 PKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAPECA 1150
            P    NS+ +WI +P+   E  ++K  +    +H A HA++ ++P  I+  + ++  EC 
Sbjct: 904  PPVIINSKGLWIDMPKYALEICQKKQLNVAGAIHGAQHAIMGMLPRFIVAGVDEIQTECK 963

Query: 1151 NP-------HDTRYFPERILLYDQHPG--GTGMSVQIQPVFIELLNAALELLTSCCCLGE 1210
             P          R  P R++ YD   G  G+G+ V+      +++ ++L  +  C C   
Sbjct: 964  APEKEFAERQTKRKRPARLIFYDSKGGKYGSGLCVKAFEHIDDIIESSLRRIEECPC--S 1014

Query: 1211 TGCPNCVQSLACHEYNEVLHKDAASLIIKGVL 1223
             GCP+CV +  C E + VL K  A +++  +L
Sbjct: 1024 DGCPDCVAASFCKENSLVLSKPGAQVVLHCIL 1014

BLAST of MC06g0251 vs. ExPASy Swiss-Prot
Match: O13983 (ATP-dependent helicase hrq1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hrq1 PE=1 SV=4)

HSP 1 Score: 454.1 bits (1167), Expect = 4.9e-126
Identity = 273/835 (32.69%), Postives = 446/835 (53.41%), Query Frame = 0

Query: 410  SSSSKSDQIQ-CH-DTSKLLPEIMVEHLKAGVGSDGQIVH--VEDIAARKANYVEIPEEL 469
            S S  +D I+ C+ +T     E ++  + +    +GQIV   +    A +A Y  +   L
Sbjct: 236  SPSIPNDSIENCNLNTKACSIEELLNEIASESSYEGQIVQEALHTYPAVEAQYGALSRPL 295

Query: 470  SNNVISAL-KCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNV 529
            S  +I+AL     +EK Y HQA +I     G HV V+T TSSGKSL Y +P+L+S+ ++ 
Sbjct: 296  SQELINALYTSRNIEKTYKHQADAINHLWNGFHVIVSTSTSSGKSLIYQIPILQSLLEDN 355

Query: 530  TSCALYLFPTKALAQDQLRSLLVMMKGFS--DNLNIGVYDGDTSQADRVLLRDNARLLIT 589
             S A ++FPTK+LAQDQ +SL+ ++       N+ +  +DGDT    R  +  +A ++ T
Sbjct: 356  QSTAFFVFPTKSLAQDQKKSLIDILSYMPTLKNIRVDTFDGDTPLESRESIIRSANIIFT 415

Query: 590  NPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSD 649
            NPDMLH +ILP   ++     NL+  ++DEAH Y G FG H A +LRR+RRI  +   S 
Sbjct: 416  NPDMLHQTILPNANRWYYFFKNLKLFVLDEAHVYNGIFGVHVAFVLRRMRRIAEYFGNSQ 475

Query: 650  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSL 709
              F+ C+AT  +P +H  ++  + +++LI    SPS  K F++WNP              
Sbjct: 476  YRFVSCSATIEDPLQHMKKIFGVDNIKLINYTSSPSGSKKFVMWNP-------------- 535

Query: 710  QSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILK- 769
                  V+ ++P       I + ++L  +  +  +R I FC+ RK CE ++   R+ LK 
Sbjct: 536  ----PYVDPKHPDDGKKSAISEASKLLIKFAEKRVRTIVFCRVRKTCESLMRLVRQELKT 595

Query: 770  ERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGF 829
            ++   L+  + +YRAGYT ++RR+IES+ F G L G+ ATNALELGID+G +DA + +GF
Sbjct: 596  KQKGDLLSKIQSYRAGYTVQERRKIESEMFNGKLYGIIATNALELGIDIGSLDAVITIGF 655

Query: 830  PGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQV 889
            P S+++L QQ GRAGRR ++SL+VY+    P+DQ+++K+P  +   P     +D  N+ +
Sbjct: 656  PYSLSNLRQQFGRAGRRNKSSLAVYIVETFPVDQFYLKHPILIHTQPNAELTLDLTNEVL 715

Query: 890  LEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEK 949
            L  HL CAA+E PI++  D+ FFG+ +     +     +++ +      K +        
Sbjct: 716  LASHLQCAAYELPINIRSDEKFFGNQIQDICEA---NLEMVEESYRPHPKYL-------P 775

Query: 950  MPSRAVSIRAIETERYRVVD--QRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLN 1009
             P+  V IR++  + + +VD    +N ++E +E  +     YEGAVY++QG+T++++ LN
Sbjct: 776  FPASQVRIRSVSEDMFTLVDVTNDKNVILELLEPFRVALTAYEGAVYVYQGKTFIIRLLN 835

Query: 1010 LSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWF 1069
            ++  +    + D+++ T  RD+TD+  +   M             T       + T   F
Sbjct: 836  INKRIITAHQVDVEWSTLQRDFTDVDPVRSLMKKTMH------GSTNIYFGAVKATLHVF 895

Query: 1070 GFYRIWKGSKQIFDTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHAL 1129
            G++++ K  K I D VD++      +S+  WI VP  + E +  K  +  A +HAA HAL
Sbjct: 896  GYFKVNK-QKDILDVVDITDHPVEIDSRGFWIDVPWHIIEVLSLKKINGAASIHAAQHAL 955

Query: 1130 LNVVPLRIICNMSDLAPEC-------ANPHDTRYFPERILLYDQ--HPGGTGMSVQIQPV 1189
            L+++P+ I  + +D+  EC             R  P R++ YD      G G+  +    
Sbjct: 956  LSLMPIFISNSGNDIRTECKAGEKEYKEAKSERRRPSRLIFYDNCGDSSGAGLCNKAYEH 1015

Query: 1190 FIELLNAALELLTSCCCLGETGCPNCVQSLACHE---YNEVLHKDAASLIIKGVL 1223
              EL+  A+E + SC C    GCP C+ S          EVL K  A +++K +L
Sbjct: 1016 TDELITMAIERIESCDCKVREGCPGCITSSKFEGGVCSGEVLDKVGALILLKMLL 1035

BLAST of MC06g0251 vs. ExPASy Swiss-Prot
Match: P50830 (Uncharacterized ATP-dependent helicase YprA OS=Bacillus subtilis (strain 168) OX=224308 GN=yprA PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.9e-115
Identity = 249/756 (32.94%), Postives = 407/756 (53.84%), Query Frame = 0

Query: 445  IVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMT 504
            +V+  +I  R+A    +PE +   + +AL   G+++LY+HQ  + +    G+ +   T T
Sbjct: 19   VVNWHEIEPREAKTRPMPESIDERIKAALSKRGIDELYTHQYSAFQYVQKGESIVTVTPT 78

Query: 505  SSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGD 564
            +SGK+LCYNLPVL+S++Q+ T+ ALYLFPTKALAQDQ   L  ++     ++    YDGD
Sbjct: 79   ASGKTLCYNLPVLQSIAQDETNRALYLFPTKALAQDQKSELNEIIDEMGIDIKSFTYDGD 138

Query: 565  TSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHT 624
            TS A R  +R    ++ITNPDMLH +ILP+H ++  +  NL++++IDE HTY+G FG H 
Sbjct: 139  TSPAIRQKVRKAGHIVITNPDMLHSAILPHHTKWVSLFENLKYIVIDELHTYRGVFGSHV 198

Query: 625  ALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFI 684
            A ++RRL+RIC   YGSDP FI  +AT ANP+E   +L     + L++++G+PS RK F+
Sbjct: 199  ANVIRRLKRIC-RFYGSDPVFICTSATIANPKELGEQLTG-KPMRLVDDNGAPSGRKHFV 258

Query: 685  LWNPIKALKNYQRDIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCK 744
             +NP                  K +N R           ++  L  E +++ ++ I F +
Sbjct: 259  FYNP--------------PIVNKPLNIRRS------ATAEVNELAKEFLKNKVQTIVFAR 318

Query: 745  TRKLCELVLCYTREILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNAL 804
            +R   E++L + +E++K+      +S+  YR GY  ++RR IE     G++ GV +TNAL
Sbjct: 319  SRVRVEIILSHIQELVKKEIG--TKSIRGYRGGYLPKERREIERGLREGDILGVVSTNAL 378

Query: 805  ELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKL 864
            ELG+D+G +   +  G+PGS+AS WQQAGRAGRR   SL + VA   P+DQY +++PE  
Sbjct: 379  ELGVDIGQLQVCVMTGYPGSVASAWQQAGRAGRRHGESLIIMVANSTPIDQYIVRHPEYF 438

Query: 865  FGSPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPD 924
            F    E   I+  N  +L  HL CAA+E P     D+ F    ++  L  L+    L   
Sbjct: 439  FNRSPESARINPENLIILVDHLKCAAYELPFRA--DEEFGAMEVSDILEYLQEEAVL--- 498

Query: 925  PSCGSSKSIWNYIGQEKMPSRAVSIRAIETERYRVVDQ---RQNEVIEEIEESKAFFQVY 984
                  +  W     E  P+  +S+R+   E   +VDQ       +I E++   A   ++
Sbjct: 499  -HRNGERYHW---ASESFPASNISLRSASQENVVIVDQSDIANVRIIGEMDRFSAMTLLH 558

Query: 985  EGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIP 1044
            + A+Y+H+G  Y V+ L+     A+  + D++YYT       + V+  +    +      
Sbjct: 559  DEAIYLHEGVQYQVEKLDWDHKKAYVRKVDVEYYTDANLAVQLKVLEIDKTKEK------ 618

Query: 1045 PSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVDLSLPKYSYNSQAVWIPVPQSVKEEV 1104
             S+T+    D  V      F +I   + +   +  + LP+   ++ A W+ + ++  E++
Sbjct: 619  -SRTSLHYGDVTVNALPTIFKKIKMTTFENIGSGPIHLPEEELHTSAAWLEI-KTADEDI 678

Query: 1105 KRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGT 1164
              K+ +    L   S+ L ++VP+ I+C+ +D+             P  I LYD +PGG 
Sbjct: 679  GEKTLE--QLLLGISNVLQHIVPVYIMCDRNDVHVVSQIKAAHTGLP-TIFLYDHYPGGI 728

Query: 1165 GMSVQIQPVFIELLNAALELLTSCCCLGETGCPNCV 1198
            G++ ++   F ++  AA +L+T C C    GCP+C+
Sbjct: 739  GLAEEVFKRFSDINEAAKQLITHCPC--HDGCPSCI 728

BLAST of MC06g0251 vs. ExPASy Swiss-Prot
Match: Q58969 (Uncharacterized ATP-dependent helicase MJ1574 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1574 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 2.0e-42
Identity = 151/593 (25.46%), Postives = 270/593 (45.53%), Query Frame = 0

Query: 440 GSDGQIVHVEDIAARKANY--VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKH 499
           G + +I+ +  I  RK  +   +   +  N +I AL      KLY HQ ++++     K 
Sbjct: 7   GIEKEIIKIYKIPERKGRFSNFKFKNKEINELIDALGF----KLYLHQVKALKYLYNKKD 66

Query: 500 VAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGF----S 559
           V V T T+SGKS  + L + ++   N     L ++PT+AL  +Q     +  + F    +
Sbjct: 67  VVVTTSTASGKSEIFRLAIFDNFLSNPDDRYLLIYPTRALINNQYEKFSMENELFYKITN 126

Query: 560 DNLNIGVYDGDTS-QADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVLIDE 619
             +   +  GD   +  R +L+D   +L T PDMLH  IL  H  +  +L NL+ +++DE
Sbjct: 127 KRVKAEILTGDVGLEKRREILKDKPNVLFTTPDMLHYQILKNHNNYLWLLKNLKLLVVDE 186

Query: 620 AHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIE 679
            H Y+G FG +   + +RL ++   +  ++   +  +AT  NP+E    L N    E+++
Sbjct: 187 LHVYRGVFGTNMVYVFKRLLKLLKRL-NNNLQILCLSATLKNPKEFVKLLFN-RDFEVVD 246

Query: 680 NDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEM 739
              +PS+RK   +  P        +++D+ Q                     + RL   +
Sbjct: 247 KSYNPSSRKYLAILEP--------KNLDNKQL--------------------LRRLIENL 306

Query: 740 VQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFG 799
           V + ++ + F  TRK  E ++   R +L  +   +   +  Y+       R  IE  F  
Sbjct: 307 VDNNIKTLVFFDTRKETEKLM---RFLLNSK---VFYKLSTYKGTLPKYVREEIEEKFKN 366

Query: 800 GNLCGVAATNALELGIDVGHIDATLHLGF-PGSIASLWQQAGRAGRRERTSLSVYVAFDG 859
           G +  +  TNALELGID+G +DA ++ G  P  I SL Q+ GRAGRR++ +L++ V    
Sbjct: 367 GEILALLTTNALELGIDIGDLDAVINYGIPPDGIFSLIQRFGRAGRRDKEALNIIVLRKD 426

Query: 860 PLDQYFMKNPEKLF----GSPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQNFFGSG 919
            LD Y+ ++  +L+       IE   ++  N+ V ++HL     E  I    + N F   
Sbjct: 427 GLDYYYKEHLNELYERIRKGIIEYMPVNIKNRFVTKKHLHYLISELKIVDFDELNDFEKE 486

Query: 920 LNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETERY---------- 979
           +   +  L+  G +    +  ++K+    I   K P  + SIR    E Y          
Sbjct: 487 I---VKELEREGKIKIYKNPITNKT---EIRNVKQPIYS-SIRTASDESYYLILDKPWIK 546

Query: 980 -RVVDQRQNE-------------VIEEIEESKAFFQVYEGAVYMHQGRTYLVK 997
            +++++ Q+E             VIEE+++ + +  +  G  Y  +G+ ++ K
Sbjct: 547 SKLLNKTQSEILSFINWLKIKGYVIEEVDKDEYYRSLITGMPYFSRGKLFIAK 552

BLAST of MC06g0251 vs. ExPASy Swiss-Prot
Match: Q57742 (Uncharacterized ATP-dependent helicase MJ0294 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0294 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 5.2e-19
Identity = 127/541 (23.48%), Postives = 230/541 (42.51%), Query Frame = 0

Query: 485 QARSIEASLAGKHVAVATMTSSGKSLC-----YNLPVLESMSQNVTS--CALYLFPTKAL 544
           Q  +I+    GK+V + + T SGK+L       N  +  SM   +      LY+ P +AL
Sbjct: 39  QRYAIKEIHEGKNVLICSPTGSGKTLSAFLAGINELIKLSMENKLEDRIYILYVSPLRAL 98

Query: 545 AQDQLRSLLVMMKGF----------SDNLNIGVYDGDTSQADRV-LLRDNARLLITNPDM 604
             D  R+L   +K             D + + V   DT+ + +  +L+    +LIT P+ 
Sbjct: 99  NNDIERNLKEPLKEIYDVAKEIGIELDEIRVAVRTSDTTSSQKQRMLKKPPHILITTPES 158

Query: 605 LHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFI 664
           L +++     +FS++LS +++V++DE H      G H +L L RL RI + +       I
Sbjct: 159 LAIAL--NSPKFSQLLSGIKYVIVDEIHALTNKRGVHLSLSLERLNRIANFIR------I 218

Query: 665 FCTATSANPREHCMEL-GNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQST 724
             +AT     E    L GN     +++                     +Y+++I+    +
Sbjct: 219 GLSATIHPLTEVAKFLVGNGRDCYIVD--------------------VSYKKEIEIKVIS 278

Query: 725 EKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAP 784
             +     P +++S     +  L  ++++     + F  TR   E V  Y +++      
Sbjct: 279 PVDDFIYTPSEEIS---KRLYNLLKKLIEEHKTTLIFTNTRSATERVAFYLKQL------ 338

Query: 785 HLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSI 844
             V+ V  + +  + E R  +E     G +    ++ +LELG+D+G ID  + LG P S+
Sbjct: 339 -GVEKVETHHSSLSREHRLEVEEKLKKGEIRVCISSTSLELGVDIGSIDLVILLGSPKSV 398

Query: 845 ASLWQQAGRAGRR-ERTSLSVYVAFDGP--LDQYFMKNPEKLFGSPIECCHIDAGNQQVL 904
           +   Q+ GR+G R    S  + + FD    ++   +    K+    I+  HI      VL
Sbjct: 399 SRALQRIGRSGHRLHEKSKGIIIPFDRDDLVENVVLAYDAKI--GKIDRIHIPKNCLDVL 458

Query: 905 EQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCG-SSKSIWN--YIGQ 964
            QHL+  A E    V    N           S K+  D++   + G   K+++   ++  
Sbjct: 459 AQHLVGMALEKVWDVDEAYNLIKKAYPYKDLSKKDFLDVLNYLAGGIEEKNVYAKIWLKD 518

Query: 965 EKMPSRAVSIRAIETERYRVV-DQRQNEVIE------EIEESKAFFQVYEGAVYMHQGRT 994
            K   R  S+RAI       + D+   +VI       E+EE  A  ++ +G +++  G+T
Sbjct: 519 NKFGKRGKSVRAIYYMNVGTIPDETAVDVIADGKYVGEVEEEFA-EKLMKGDIFVLGGKT 538

BLAST of MC06g0251 vs. NCBI nr
Match: XP_022135054.1 (uncharacterized protein LOC111007140 isoform X1 [Momordica charantia] >XP_022135056.1 uncharacterized protein LOC111007140 isoform X1 [Momordica charantia])

HSP 1 Score: 2427 bits (6290), Expect = 0.0
Identity = 1222/1238 (98.71%), Postives = 1222/1238 (98.71%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
            KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140

Query: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200
            DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG
Sbjct: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200

Query: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1233

BLAST of MC06g0251 vs. NCBI nr
Match: XP_022135057.1 (uncharacterized protein LOC111007140 isoform X2 [Momordica charantia])

HSP 1 Score: 2297 bits (5953), Expect = 0.0
Identity = 1170/1238 (94.51%), Postives = 1170/1238 (94.51%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQ                                 
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQ--------------------------------- 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
                               AYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 -------------------AYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140

Query: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200
            DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG
Sbjct: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1181

Query: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1181

BLAST of MC06g0251 vs. NCBI nr
Match: XP_022135058.1 (uncharacterized protein LOC111007140 isoform X3 [Momordica charantia])

HSP 1 Score: 2206 bits (5717), Expect = 0.0
Identity = 1117/1134 (98.50%), Postives = 1118/1134 (98.59%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
            KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLR 1126
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPL+
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLK 1129

BLAST of MC06g0251 vs. NCBI nr
Match: XP_038878313.1 (uncharacterized ATP-dependent helicase YprA isoform X7 [Benincasa hispida])

HSP 1 Score: 2094 bits (5426), Expect = 0.0
Identity = 1058/1234 (85.74%), Postives = 1129/1234 (91.49%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEES REI I+TLTGESLTV ISG+RTIDDLKLLLRRNFPSAT SPNFHLF K  KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            S I ACRIDHGEFLVLIPFNKKESSKS+LRDQYEQ SSVS  SSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN- 180
            SYLH CS + R+EN  E ERG+SE+G V+AELV T S+SS SSK+KGKKGF ++DLKGN 
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHR 240
            DDVLR   SS +EGFLNE TCE+F+K+LESVDCLSDPRNG CMLAKQANSR  NK+ P+ 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDI 300
            T   SCLCPVWLKK  KAFSFLNVF+M LQLQE+IMTVSRLEQAMD L+  GIT+C EDI
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDG--NTGK-DVTLLKRRERS 360
            ++LSLLCPKAVHFASG  ED+C D L I+IYL+E+NGRW D   NT   +VT LKRRERS
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTAPTNVTPLKRRERS 360

Query: 361  FKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSSSKSD 420
            FKFYL EAIK HML+HGSR EICVPFSLE LITP E+ V G E KR KK DTASSS +SD
Sbjct: 361  FKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKRGKKCDTASSS-QSD 420

Query: 421  QIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISALKCI 480
            + QCHDTSKLLPE MVEHL+ GVGS+GQIVHVEDI ARKANYVEIP+ELSNNV+SALKCI
Sbjct: 421  RRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIPKELSNNVVSALKCI 480

Query: 481  GVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKA 540
            GV  LYSHQA+SIEASLAGKHVAVATMTSSGKSLCYNLPVLE+MSQNV+SCALYLFPTKA
Sbjct: 481  GVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQNVSSCALYLFPTKA 540

Query: 541  LAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHR 600
            LAQDQLRSLL+MMKGF+ NLNIGVYDGDTSQADR+LLRDNARLLITNPDMLHVSILP+HR
Sbjct: 541  LAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLITNPDMLHVSILPHHR 600

Query: 601  QFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPR 660
            QFSRILSNLRF++IDEAHTYKGAFGCHTALILRRLRR+CSHVYGSDPSFIFCTATSANPR
Sbjct: 601  QFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSDPSFIFCTATSANPR 660

Query: 661  EHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFRNPRQ 720
            EHCMELGNLSSLELIENDGSPSARKLFILWNPI ALKNYQR IDS QST+KN+  RNP  
Sbjct: 661  EHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSPQSTKKNMTVRNPS- 720

Query: 721  QLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAYRA 780
                PIMDIARLFAEMV HGLRCIAFCKTRKLCELVLCYTREILKE APHLVQSVCAYRA
Sbjct: 721  ----PIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKESAPHLVQSVCAYRA 780

Query: 781  GYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAG 840
            GYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAG
Sbjct: 781  GYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAG 840

Query: 841  RRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHEHPIS 900
            RRE+TSLSVYVAF+GPLDQYF+K+PEKLFGSPIECCHIDA NQQVLEQHLLCAA+EHP+ 
Sbjct: 841  RREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVLEQHLLCAAYEHPVC 900

Query: 901  VLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETER 960
            V YDQNFFG GLNTAL SLKNRGDLI + SCGSSKSIWNYIGQEKMPSR+VSIRAIE ER
Sbjct: 901  VAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKMPSRSVSIRAIEAER 960

Query: 961  YRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYT 1020
            Y+VVDQRQNE++EEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLS+MLAFCEEADLKYYT
Sbjct: 961  YKVVDQRQNEILEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKYYT 1020

Query: 1021 KTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVD 1080
            KTRDYTDIHVI GN+AYPRR PNIPPSKTTAQANDCRVTTTWFGFY+I KGS QI DT++
Sbjct: 1021 KTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFYQIQKGSNQIIDTLN 1080

Query: 1081 LSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAP 1140
            LSLPKYSYNSQAVW+PVPQS+KEEVKRK+FDFRAGLHAASHALLNVVPLRIICNMSDLAP
Sbjct: 1081 LSLPKYSYNSQAVWVPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPLRIICNMSDLAP 1140

Query: 1141 ECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETGCPNC 1200
            ECANPHDTRYFPERILLYDQHPGGTG+S+QIQPVFIELLNAA ELLTSC CLGETGCPNC
Sbjct: 1141 ECANPHDTRYFPERILLYDQHPGGTGLSLQIQPVFIELLNAAFELLTSCRCLGETGCPNC 1200

Query: 1201 VQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            VQSL CHEYNEVLHKDAASLIIKGVLDAEK+YCR
Sbjct: 1201 VQSLTCHEYNEVLHKDAASLIIKGVLDAEKAYCR 1228

BLAST of MC06g0251 vs. NCBI nr
Match: XP_038878311.1 (uncharacterized ATP-dependent helicase YprA isoform X5 [Benincasa hispida])

HSP 1 Score: 2088 bits (5410), Expect = 0.0
Identity = 1058/1248 (84.78%), Postives = 1129/1248 (90.46%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEES REI I+TLTGESLTV ISG+RTIDDLKLLLRRNFPSAT SPNFHLF K  KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            S I ACRIDHGEFLVLIPFNKKESSKS+LRDQYEQ SSVS  SSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN- 180
            SYLH CS + R+EN  E ERG+SE+G V+AELV T S+SS SSK+KGKKGF ++DLKGN 
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHR 240
            DDVLR   SS +EGFLNE TCE+F+K+LESVDCLSDPRNG CMLAKQANSR  NK+ P+ 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDI 300
            T   SCLCPVWLKK  KAFSFLNVF+M LQLQE+IMTVSRLEQAMD L+  GIT+C EDI
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDG--NT-------------- 360
            ++LSLLCPKAVHFASG  ED+C D L I+IYL+E+NGRW D   NT              
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTVTSHRCCTSCKSFT 360

Query: 361  -GKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKR 420
               +VT LKRRERSFKFYL EAIK HML+HGSR EICVPFSLE LITP E+ V G E KR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  RKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIP 480
             KK DTASSS +SD+ QCHDTSKLLPE MVEHL+ GVGS+GQIVHVEDI ARKANYVEIP
Sbjct: 421  GKKCDTASSS-QSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNV+SALKCIGV  LYSHQA+SIEASLAGKHVAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLIT 600
            NV+SCALYLFPTKALAQDQLRSLL+MMKGF+ NLNIGVYDGDTSQADR+LLRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSD 660
            NPDMLHVSILP+HRQFSRILSNLRF++IDEAHTYKGAFGCHTALILRRLRR+CSHVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSL 720
            PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPI ALKNYQR IDS 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKE 780
            QST+KN+  RNP      PIMDIARLFAEMV HGLRCIAFCKTRKLCELVLCYTREILKE
Sbjct: 721  QSTKKNMTVRNPS-----PIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKE 780

Query: 781  RAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP 840
             APHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP
Sbjct: 781  SAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP 840

Query: 841  GSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVL 900
            GSIASLWQQAGRAGRRE+TSLSVYVAF+GPLDQYF+K+PEKLFGSPIECCHIDA NQQVL
Sbjct: 841  GSIASLWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVL 900

Query: 901  EQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKM 960
            EQHLLCAA+EHP+ V YDQNFFG GLNTAL SLKNRGDLI + SCGSSKSIWNYIGQEKM
Sbjct: 901  EQHLLCAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKM 960

Query: 961  PSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSS 1020
            PSR+VSIRAIE ERY+VVDQRQNE++EEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLS+
Sbjct: 961  PSRSVSIRAIEAERYKVVDQRQNEILEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLST 1020

Query: 1021 MLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFY 1080
            MLAFCEEADLKYYTKTRDYTDIHVI GN+AYPRR PNIPPSKTTAQANDCRVTTTWFGFY
Sbjct: 1021 MLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFY 1080

Query: 1081 RIWKGSKQIFDTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNV 1140
            +I KGS QI DT++LSLPKYSYNSQAVW+PVPQS+KEEVKRK+FDFRAGLHAASHALLNV
Sbjct: 1081 QIQKGSNQIIDTLNLSLPKYSYNSQAVWVPVPQSIKEEVKRKNFDFRAGLHAASHALLNV 1140

Query: 1141 VPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELL 1200
            VPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTG+S+QIQPVFIELLNAA ELL
Sbjct: 1141 VPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGLSLQIQPVFIELLNAAFELL 1200

Query: 1201 TSCCCLGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            TSC CLGETGCPNCVQSL CHEYNEVLHKDAASLIIKGVLDAEK+YCR
Sbjct: 1201 TSCRCLGETGCPNCVQSLTCHEYNEVLHKDAASLIIKGVLDAEKAYCR 1242

BLAST of MC06g0251 vs. ExPASy TrEMBL
Match: A0A6J1BZJ1 (uncharacterized protein LOC111007140 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2427 bits (6290), Expect = 0.0
Identity = 1222/1238 (98.71%), Postives = 1222/1238 (98.71%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
            KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140

Query: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200
            DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG
Sbjct: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200

Query: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1233

BLAST of MC06g0251 vs. ExPASy TrEMBL
Match: A0A6J1C1K3 (uncharacterized protein LOC111007140 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2297 bits (5953), Expect = 0.0
Identity = 1170/1238 (94.51%), Postives = 1170/1238 (94.51%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQ                                 
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQ--------------------------------- 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
                               AYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 -------------------AYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMS 1140

Query: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1200
            DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG
Sbjct: 1141 DLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETG 1181

Query: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1230
            CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 CPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1181

BLAST of MC06g0251 vs. ExPASy TrEMBL
Match: A0A6J1C3R1 (uncharacterized protein LOC111007140 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2206 bits (5717), Expect = 0.0
Identity = 1117/1134 (98.50%), Postives = 1118/1134 (98.59%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK  KLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180
            SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGND 180

Query: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240
            DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT
Sbjct: 181  DVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRT 240

Query: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300
            YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK
Sbjct: 241  YSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIK 300

Query: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK--------DVTLLKR 360
            HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGK        DVTLLKR
Sbjct: 301  HLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLKR 360

Query: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420
            RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS
Sbjct: 361  RERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSS 420

Query: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480
            SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA
Sbjct: 421  SKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISA 480

Query: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540
            LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF
Sbjct: 481  LKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLF 540

Query: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600
            PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL
Sbjct: 541  PTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSIL 600

Query: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660
            PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS
Sbjct: 601  PYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATS 660

Query: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720
            ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR
Sbjct: 661  ANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFR 720

Query: 721  NPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780
            NP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC
Sbjct: 721  NPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVC 780

Query: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840
            AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA
Sbjct: 781  AYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQA 840

Query: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900
            GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE
Sbjct: 841  GRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHE 900

Query: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960
            HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI
Sbjct: 901  HPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAI 960

Query: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020
            ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL
Sbjct: 961  ETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADL 1020

Query: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080
            KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF
Sbjct: 1021 KYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIF 1080

Query: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLR 1126
            DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPL+
Sbjct: 1081 DTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLK 1129

BLAST of MC06g0251 vs. ExPASy TrEMBL
Match: A0A6J1L0Y0 (uncharacterized protein LOC111500077 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500077 PE=4 SV=1)

HSP 1 Score: 2077 bits (5381), Expect = 0.0
Identity = 1044/1237 (84.40%), Postives = 1120/1237 (90.54%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQ 60
            MEESE EIRI+TLTGESLT+ ISG RTI+DLKLLLR+NFPSAT SPNFHLFSK  KLKPQ
Sbjct: 1    MEESESEIRIKTLTGESLTISISGDRTIEDLKLLLRKNFPSATVSPNFHLFSKGTKLKPQ 60

Query: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120
            S I ACRID GEFLVLIPF KKESSK QLRDQYEQ SSVS  SSIS FADSAWSDMVQDL
Sbjct: 61   SEISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSISLFADSAWSDMVQDL 120

Query: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN- 180
            SYLHDCS+QGR+ N  E ERGN E G V++EL  T ++  SS KA+GKKGF+ ND  G  
Sbjct: 121  SYLHDCSVQGRERNGLESERGNFETGGVDSELAATCTTGPSSLKARGKKGFICNDSNGIL 180

Query: 181  DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHR 240
            DD+LRNLLSS T G L+E+ CEN +KFLESVDCLSDPRN +C+LAKQANSR  ++K P+R
Sbjct: 181  DDILRNLLSSPTVGLLSEYNCENLIKFLESVDCLSDPRNEKCLLAKQANSRSGSRKAPNR 240

Query: 241  TYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDI 300
            T   SCLCPVWLKKI K F+FLNV SM  QL+E+I+T SRLEQAMDLLQ HGIT+  ED+
Sbjct: 241  TRCSSCLCPVWLKKIMKTFAFLNVLSMFAQLREEIITASRLEQAMDLLQKHGITLRMEDM 300

Query: 301  KHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTG--------KDVTLLK 360
            KHLSLLCPK VHFASG+LEDS DD + IV YL+ QN RW   NT          D+T +K
Sbjct: 301  KHLSLLCPKVVHFASGTLEDSYDDKIIIVNYLTAQNSRWTADNTAYKLSSKAPTDITPMK 360

Query: 361  RRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASS 420
            RRE+SFKFYLWEAIK HMLRHGSRSEICV FSLEDLIT K S+V G E KR KKSD ASS
Sbjct: 361  RREKSFKFYLWEAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDGNEAKRAKKSDMASS 420

Query: 421  SSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVIS 480
            SS+SD++QCHDTSKLLPE MVEHL+ G+GS+GQIVHVEDIAARKANYVEIPEELSNNVIS
Sbjct: 421  SSQSDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKANYVEIPEELSNNVIS 480

Query: 481  ALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYL 540
            ALKCIGVEKLYSHQ RSIEASLAG HVAVATMTSSGKSLCYNLPVLESMSQ+V+SCALYL
Sbjct: 481  ALKCIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVLESMSQDVSSCALYL 540

Query: 541  FPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSI 600
            FPTKALAQDQLRSLLVMMKGF+ +LNIGVYDGDTSQ+DR+LLRDNARLLITNPDMLH+SI
Sbjct: 541  FPTKALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNARLLITNPDMLHLSI 600

Query: 601  LPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTAT 660
            LP HRQFSRILSNLRF++IDEAHTYKGAFGCHTALI+RRLRR+CSHVYGSDPSFIFCTAT
Sbjct: 601  LPQHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSHVYGSDPSFIFCTAT 660

Query: 661  SANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNF 720
            SANPR+HCMELG+LSSLELIENDGSPSARKLFILWNPI A K+ QR IDSLQSTEKN NF
Sbjct: 661  SANPRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSQRGIDSLQSTEKNANF 720

Query: 721  RNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSV 780
            RNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSV
Sbjct: 721  RNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSV 780

Query: 781  CAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQ 840
            CAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQ
Sbjct: 781  CAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQ 840

Query: 841  AGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAH 900
            AGRAGRRE+TSLSVYVAF+GPLDQYFMK+PEKLFGSPI+CCHIDA NQQVLEQHLLCAA+
Sbjct: 841  AGRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIKCCHIDAENQQVLEQHLLCAAY 900

Query: 901  EHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRA 960
            EHP+ ++YDQN FG GLNTAL SLK+RGDLIP PSCGSSKSIW YIG+EKMPSR+VSIRA
Sbjct: 901  EHPVCMVYDQNLFGPGLNTALMSLKSRGDLIPAPSCGSSKSIWTYIGKEKMPSRSVSIRA 960

Query: 961  IETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEAD 1020
            IE ERY+VVDQRQNEV+EEIEES AFFQVYEGAVYMHQGRTYLVKSLNLS+MLAFCEEAD
Sbjct: 961  IEAERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEAD 1020

Query: 1021 LKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQI 1080
            LKYYTKTRDYTDIHVIGG++AYPRR PNIP  KTTAQANDCRVTTTWFGFYRIWKGS QI
Sbjct: 1021 LKYYTKTRDYTDIHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTTWFGFYRIWKGSNQI 1080

Query: 1081 FDTVDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNM 1140
            FDTVDLSLPKYSYNSQAVWIPVP S+KEEVKRK++DFRAGLHAASHALLNVVPLRIICNM
Sbjct: 1081 FDTVDLSLPKYSYNSQAVWIPVPLSIKEEVKRKNYDFRAGLHAASHALLNVVPLRIICNM 1140

Query: 1141 SDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGET 1200
            SDLAPECANPHD+RYFPERILLYDQHPGGTGMS+QIQPVFIELLNAALELLTSCCC GET
Sbjct: 1141 SDLAPECANPHDSRYFPERILLYDQHPGGTGMSLQIQPVFIELLNAALELLTSCCCFGET 1200

Query: 1201 GCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSY 1228
            GCPNCVQSLACHEYNEVLHKDAASLIIKGVLD EK+Y
Sbjct: 1201 GCPNCVQSLACHEYNEVLHKDAASLIIKGVLDTEKTY 1232

BLAST of MC06g0251 vs. ExPASy TrEMBL
Match: A0A6J1F581 (uncharacterized protein LOC111440956 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440956 PE=4 SV=1)

HSP 1 Score: 2073 bits (5372), Expect = 0.0
Identity = 1043/1234 (84.52%), Postives = 1121/1234 (90.84%), Query Frame = 0

Query: 3    ESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQSL 62
            ESE EIRI+TLTGESLT+ ISG+RTI+DLKLLLR+NFPSAT SPNFHLFSK  KLKPQS 
Sbjct: 5    ESESEIRIKTLTGESLTISISGNRTIEDLKLLLRKNFPSATVSPNFHLFSKGTKLKPQSE 64

Query: 63   IGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDLSY 122
            I ACRID GEFLVLIPF KKESSK QLRDQYEQ SSVS  SSISQFADSAWSDMVQDLSY
Sbjct: 65   ISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSISQFADSAWSDMVQDLSY 124

Query: 123  LHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN-DD 182
            LHDCS+QGR+ N  E ERGN E G V+AEL  T S+ SSS KAKGKKGFV ND  G  DD
Sbjct: 125  LHDCSVQGREGNGLESERGNFEAGGVDAELAATCSTGSSSLKAKGKKGFVCNDSNGILDD 184

Query: 183  VLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRTY 242
            +LRNLLSS T  FL+E+ CEN +K LESVDCLSDPRN +C+LAKQANSR  ++K P+ T 
Sbjct: 185  ILRNLLSSPTVEFLSEYNCENLIKLLESVDCLSDPRNEKCLLAKQANSRSGSRKAPNGTR 244

Query: 243  SPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIKH 302
              SCLCPVWLKKI KAF+FLNV SM  QL+E+I+T SRLEQA+DLLQ HGIT+  ED+KH
Sbjct: 245  CSSCLCPVWLKKIMKAFAFLNVLSMFAQLREEIITASRLEQAIDLLQKHGITLRMEDMKH 304

Query: 303  LSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTG-------KDVTLLKRRE 362
            LSLLCPK VHFASG+LEDS DD + IVIYL+ QN RW   NT         D+T LKRRE
Sbjct: 305  LSLLCPKVVHFASGTLEDSYDDKIIIVIYLTAQNSRWTADNTAYKLSKGPTDITPLKRRE 364

Query: 363  RSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASSSSK 422
            +SFKFYLW+AIK HMLRHGSRSEICV FSLEDLIT K S+V G E KR KKSD ASSSSK
Sbjct: 365  KSFKFYLWDAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDGNEAKRAKKSDMASSSSK 424

Query: 423  SDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISALK 482
            SD++QCHDTSKLLPE MVEHL+ G+GS+GQIVHVEDIAARKANYVEIPEELSNNVISALK
Sbjct: 425  SDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKANYVEIPEELSNNVISALK 484

Query: 483  CIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPT 542
            CIGVEKLYSHQ RSIEASLAG HVAVATMTSSGKSLCYNLPVLESMSQ+V+SCALYLFPT
Sbjct: 485  CIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVLESMSQDVSSCALYLFPT 544

Query: 543  KALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSILPY 602
            KALAQDQLRSLLVMMKGF+ +LNIGVYDGDTSQ+DR+LLRDNARLLITNPDMLH+SILP 
Sbjct: 545  KALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNARLLITNPDMLHLSILPQ 604

Query: 603  HRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSAN 662
            HRQFSRILSNLRF++IDEAHTYKGAFGCHTALI+RRLRR+CSHVYGSDPSFIFCTATSAN
Sbjct: 605  HRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSHVYGSDPSFIFCTATSAN 664

Query: 663  PREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFRNP 722
            PR+HCMELG+LSSLELIENDGSPSARKLFILWNPI A K+ +R IDSLQSTEKN NFRNP
Sbjct: 665  PRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSERGIDSLQSTEKNANFRNP 724

Query: 723  RQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY 782
                  PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY
Sbjct: 725  S-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY 784

Query: 783  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR 842
            RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR
Sbjct: 785  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR 844

Query: 843  AGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHEHP 902
            AGRRE+TSLSVYVAF+GPLDQYFMK+PEKLFGSPIECCHIDA NQQVLE HLLCAA+EHP
Sbjct: 845  AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEHHLLCAAYEHP 904

Query: 903  ISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIET 962
            + ++YDQN FG GLNTAL SLK+RGDL+P PSCGSSKSIW YIG+EKMPSR+VSIRAIE 
Sbjct: 905  VCMVYDQNLFGPGLNTALVSLKSRGDLMPAPSCGSSKSIWTYIGKEKMPSRSVSIRAIEA 964

Query: 963  ERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKY 1022
            ERY+VVDQR+NEV+EEIEES AFFQVYEGAVYMHQGRTYLVKSLNLS+MLAFCEEADLKY
Sbjct: 965  ERYKVVDQRRNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKY 1024

Query: 1023 YTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDT 1082
            YTKTRDYTD+HVIGG++AYPRR PNIP  KTTAQANDCRVTTTWFGFYRIWKGS QIFDT
Sbjct: 1025 YTKTRDYTDVHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTTWFGFYRIWKGSNQIFDT 1084

Query: 1083 VDLSLPKYSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMSDL 1142
            VDLSLPKYSYNSQAVWIPVP S+KEEVKRK++DFRAGLHAASHALLNVVPLRIICNMSDL
Sbjct: 1085 VDLSLPKYSYNSQAVWIPVPLSIKEEVKRKNYDFRAGLHAASHALLNVVPLRIICNMSDL 1144

Query: 1143 APECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSCCCLGETGCP 1202
            APECANPHD+RYFPERILLYDQHPGGTG+S+QIQPVFIELL+AALELLTSCCC GETGCP
Sbjct: 1145 APECANPHDSRYFPERILLYDQHPGGTGVSLQIQPVFIELLHAALELLTSCCCFGETGCP 1204

Query: 1203 NCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSY 1228
            NCVQSLACHEYNEVLHKDAASLIIKGVLDAEK+Y
Sbjct: 1205 NCVQSLACHEYNEVLHKDAASLIIKGVLDAEKTY 1233

BLAST of MC06g0251 vs. TAIR 10
Match: AT5G08110.1 (nucleic acid binding;ATP-dependent helicases;ATP binding;helicases;ATP-dependent helicases )

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 633/1226 (51.63%), Postives = 802/1226 (65.42%), Query Frame = 0

Query: 8    IRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAIKLKPQSLIGACR 67
            I +R++ GES TV +S   TI DLK  L+  FP A++S NFHL+ K  KLK  + + A  
Sbjct: 15   ISVRSINGESTTVQVSSDGTIRDLKKALKSCFPPASSSSNFHLYIKGEKLKLDTRVAAIT 74

Query: 68   IDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDLSYLHDCS 127
            I+ G+ L L PF KKE  ++   D   + SS+S R+S                       
Sbjct: 75   INDGDILGLFPFKKKELRQTPKPD-LSKPSSLSSRTSTM--------------------- 134

Query: 128  LQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGNDDVLRNLL 187
               +DEN              E   VG        +   G     FND         +L 
Sbjct: 135  ---KDENAKR----------AEDHCVGEKRKRDEEACPYG----FFND---------DLE 194

Query: 188  SSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHRTYSPSCLC 247
            S   + F  ++T E   + L+S +CL+ P + +C+++  ++             S  C C
Sbjct: 195  SECKDAFKGQNT-EKLAEVLKSRNCLTSPGSTKCLMSWDSS-------------SSLCSC 254

Query: 248  PVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDIKHLSLLCP 307
            P W    R A S                               G+ +  +D+K+LS++CP
Sbjct: 255  PDWESLSRLAMS-------------------------------GVRVRIQDVKNLSVICP 314

Query: 308  KAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKDVTLLKRRERSFKFYLWEAIKF 367
            K +     ++       ++  + + E++GR       K  + +K+RE SFK   WE+I+ 
Sbjct: 315  KVITDDYEAVNYENAIVIADYLEMDEKSGR-KKIPLAKLFSAMKKREASFKSDFWESIRS 374

Query: 368  HMLRHGSRSEICVPFSLEDLIT-PKESSVHGGEEKRRK-KSDTASSSSKSDQIQCHDTSK 427
             + ++   S I +  SLE L+    E    GG E R+  K    +S S+  Q  CH T+ 
Sbjct: 375  LLNKNTGESGIAI--SLEGLLKFASEGRADGGNEARQAGKGTCPTSGSRKFQTLCHATNS 434

Query: 428  LLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQ 487
            LLP  MVEHL+ G+GS GQ+VHVE I ARK+ YVE+ ++LS    SALK IG+  LYSHQ
Sbjct: 435  LLPSEMVEHLRNGIGSKGQVVHVEVIKARKSAYVEMSDKLSETTKSALKRIGLNTLYSHQ 494

Query: 488  ARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSL 547
            A +I A+LAGK+VAVATMTSSGKSLCYN+PV E + ++  SCALYLFPTKALAQDQLR+L
Sbjct: 495  AEAISAALAGKNVAVATMTSSGKSLCYNVPVFEELCKDTNSCALYLFPTKALAQDQLRAL 554

Query: 548  LVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHR-QFSRILSN 607
              ++KGF  ++N+GVYDGDT   DR  LR NARLLITNPDMLH+SIL  H+ QFSRILSN
Sbjct: 555  SDLIKGFEASINMGVYDGDTPYKDRTRLRSNARLLITNPDMLHISILRRHKEQFSRILSN 614

Query: 608  LRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGN 667
            LR+++IDEAH YKG FGCH ALILRRLRR+CSHVYG +PSFIFCTATSANPREHCMEL N
Sbjct: 615  LRYIVIDEAHIYKGPFGCHMALILRRLRRLCSHVYGVNPSFIFCTATSANPREHCMELAN 674

Query: 668  LSSLELIENDGSPSARKLFILWNP-IKALKNYQRDIDSLQSTEKNVNFRNPR--QQLSIP 727
            LS LEL+  DGSPS+ KLF+LWNP        ++    + S+E   +  +      LS P
Sbjct: 675  LSELELVTIDGSPSSEKLFVLWNPSAPPTSKSEKSSKVVSSSEAAADKPSGAAVDTLSGP 734

Query: 728  IMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAYRAGYTAE 787
              +++ LFAEMVQHGLRCIAFC +RKLCELVLC TREIL E APHLV+++ +YR GY AE
Sbjct: 735  ASEVSHLFAEMVQHGLRCIAFCPSRKLCELVLCLTREILAETAPHLVEAITSYRGGYIAE 794

Query: 788  DRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRRERT 847
            DRR+IESD FGG LCG+AATNALELGIDVGHID TLHLGFPGSIASLWQQAGR+GRR++ 
Sbjct: 795  DRRKIESDLFGGKLCGIAATNALELGIDVGHIDVTLHLGFPGSIASLWQQAGRSGRRQKP 854

Query: 848  SLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQ 907
            SL+VYVAF GPLDQY+M  P+KLFGSPIECCHID+ N+ VL QHL CAA EHP+S+ YDQ
Sbjct: 855  SLAVYVAFLGPLDQYYMSFPDKLFGSPIECCHIDSQNKHVLMQHLACAALEHPLSLQYDQ 914

Query: 908  NFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETERYRVVD 967
              FGSGL+  L  L+N+G L  DPS  SS  IWNYIG+EK P+R VSIRAIET RYRV++
Sbjct: 915  QHFGSGLSDPLAELRNKGFLSFDPSRDSSSRIWNYIGREKNPARIVSIRAIETVRYRVME 974

Query: 968  QRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYTKTRDY 1027
            ++  +V++EIEESKAFF VYEGA+YM+QGR YLV SL++   +A CE  ++ YYT+TRDY
Sbjct: 975  KKSKDVLDEIEESKAFFHVYEGAIYMNQGRNYLVTSLDIKEKVALCELVNVDYYTRTRDY 1034

Query: 1028 TDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVDLSLPK 1087
            TDI V GG+ AYP +     P K T Q + CRVTT WFGF RI + + ++ D V+LSLP 
Sbjct: 1035 TDIKVTGGDTAYPVK----APKKPTPQTHACRVTTKWFGFLRIRRRNNEVIDDVELSLPS 1094

Query: 1088 YSYNSQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAPECANP 1147
            Y+Y SQAVWI VP SVK  V+  +  FRAGLHAA HAL+NVVP R+ CN SD+APEC NP
Sbjct: 1095 YTYQSQAVWIQVPMSVKLAVETANLPFRAGLHAACHALVNVVPTRVTCNYSDIAPECPNP 1140

Query: 1148 HDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSC-CCLGETGCPNCVQSL 1207
             + RYFP RIL+YD+HPGGTG+S +I P+F ELL  A +LL SC  C  ETGCP C Q+ 
Sbjct: 1155 QEQRYFPARILVYDRHPGGTGISAKICPLFFELLKDARDLLRSCEKCPPETGCPKCAQTF 1140

Query: 1208 ACHEYNEVLHKDAASLIIKGVLDAEK 1227
             C  YNE+LHK AA +I++GVLDA++
Sbjct: 1215 GCRGYNELLHKKAAIMIMQGVLDAKE 1140

BLAST of MC06g0251 vs. TAIR 10
Match: AT1G27880.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 71.6 bits (174), Expect = 4.9e-12
Identity = 98/408 (24.02%), Postives = 172/408 (42.16%), Query Frame = 0

Query: 441 SDGQIVHVED-IAARKANYVEIPEELSNNVISALKCI-GVEKLYSHQARSIEASLAGKHV 500
           ++G I  VED I A K       E    N+   L  + G +     Q ++I+  L G   
Sbjct: 234 ANGFISSVEDAILAVKT------EASDENLTKLLNLVYGYDSFRDGQLQAIKMILGGSST 293

Query: 501 AVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNI 560
            +   T +GKSLCY +P +      +    L + P  +L  DQL+ L  ++KG    L+ 
Sbjct: 294 MLVLPTGAGKSLCYQIPAM-----ILPGITLVVSPLVSLMIDQLKHLPSIIKG--GLLSS 353

Query: 561 GVYDGDTSQADRVLLRDNARLLITNPD-MLHVSILPYHRQFSRILSNLRFVLIDEAH-TY 620
                + ++  R L     ++L  +P+ +L+V  L       R+  ++  V++DEAH   
Sbjct: 354 SQRPEEATETLRKLKEGIIKVLFVSPERLLNVEFL----SMFRMSLSVSLVVVDEAHCVS 413

Query: 621 KGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGS 680
           + +     + +  +   + S +       +  TAT+   +        +SSLE+      
Sbjct: 414 EWSHNFRPSYMRLKASMLFSELKAECILAMTATATTMTLQ------AVMSSLEI------ 473

Query: 681 PSARKLFILWNPIKALKNYQRDIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHG 740
           PS   +          K+  RD     + E +V+     +   + I+  +  + E+    
Sbjct: 474 PSTNLI---------QKSQLRD-----NFELSVSLSGANRMKDLLILMESPPYKEI---- 533

Query: 741 LRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCA--YRAGYTAEDRRRIESDFFGGN 800
              I +CK +   +++  Y R+           ++ A  Y +G  A+DR RI+  F    
Sbjct: 534 RSIIVYCKFQYETDMISKYLRD----------NNINAKGYHSGLPAKDRVRIQESFCSNK 584

Query: 801 LCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRRERTS 843
           +  V AT A  +G+D G + A +H   PGS+    Q+ GRAGR  R S
Sbjct: 594 IRVVVATVAFGMGLDKGDVGAVIHFSVPGSMEEYVQEIGRAGRDGRLS 584

BLAST of MC06g0251 vs. TAIR 10
Match: AT4G16630.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 50.8 bits (120), Expect = 8.9e-06
Identity = 60/239 (25.10%), Postives = 104/239 (43.51%), Query Frame = 0

Query: 448 VEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSG 507
           V+ ++     ++E+   LS  ++ A + +G +K    QA  I  +L G+ +  + +T SG
Sbjct: 159 VDGVSFHADTFMEL--NLSRPLLRACETLGYKKPTPIQAACIPLALTGRDLCASAITGSG 218

Query: 508 KSLCYNLPVLESM----SQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDG 567
           K+  + LP LE +     +   +  L L PT+ LA  Q+ S++  +  F+D +  G+  G
Sbjct: 219 KTAAFALPTLERLLFRPKRVFATRVLILTPTRELAV-QIHSMIQNLAQFTD-IKCGLIVG 278

Query: 568 DTS-QADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGC 627
             S +   V+LR    +++  P  +   I       S  L +L  +++DEA         
Sbjct: 279 GLSVREQEVVLRSMPDIVVATPGRM---IDHLRNSMSVDLDDLAVLILDEADR---LLQT 338

Query: 628 HTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARK 682
             A  +  L R+C       P        SA   E   EL  LS  + +     PSAR+
Sbjct: 339 GFATEITELVRLC-------PKRRQTMLFSATMTEEVKELVKLSLNKPLRLSADPSARR 380

BLAST of MC06g0251 vs. TAIR 10
Match: AT5G62190.1 (DEAD box RNA helicase (PRH75) )

HSP 1 Score: 45.8 bits (107), Expect = 2.9e-04
Identity = 59/247 (23.89%), Postives = 103/247 (41.70%), Query Frame = 0

Query: 387 LITPKESSVHGGEEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQ-- 446
           L TP+  S  G +E++ K SD+    S+  + +  D  +   E   + +K+   S+ +  
Sbjct: 21  LDTPELDSKKGKKEQKLKLSDSDEEESEKKKSKKKDKKRKASE-EEDEVKSDSSSEKKKS 80

Query: 447 ----IVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAV 506
                + VED+     N V     +S  +   LK  G+E L+  QA + +  L G  +  
Sbjct: 81  SKKVKLGVEDVEVDNPNAVS-KFRISAPLREKLKANGIEALFPIQASTFDMVLDGADLVG 140

Query: 507 ATMTSSGKSLCYNLPVLES-----------MSQNVTSCALYLFPTKALAQDQLRSLLVMM 566
              T  GK+L + LP+LES           M    +   L L PT+ LA    + +    
Sbjct: 141 RARTGQGKTLAFVLPILESLVNGPAKSKRKMGYGRSPSVLVLLPTRELA----KQVAADF 200

Query: 567 KGFSDNLNIG---VYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLR 614
             +  +L +    +Y GD+       L+    +++  P  +   I   +  F    S L+
Sbjct: 201 DAYGGSLGLSSCCLYGGDSYPVQEGKLKRGVDIVVGTPGRIKDHIERQNLDF----SYLQ 257