MC06g0226 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0226 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.3e-189
Identity = 334/510 (65.49%), Postives = 400/510 (78.43%), Query Frame = 0

Query: 16  AVILYRVLF-------LRRSFRS---LRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGK 75
           ++ILY V+F       LR  FR    L LPPGPKPWPI+GNL H+G  P H   AAMA  
Sbjct: 5   SLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAAMAQT 64

Query: 76  YGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGP 135
           YGPL+YL++G VDVVVAASA+VA QFLK HD NF SRPPN+GA+++AYNYQDLVFAPYGP
Sbjct: 65  YGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGP 124

Query: 136 RWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALG 195
           RWR+LRKI +VHLFSTKA+D  R VR++EV  L  A+A AG  P  LG LL +CT NAL 
Sbjct: 125 RWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALA 184

Query: 196 RTMIGRRVF----GGADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKK 255
           R M+G+RVF    G  DP+   FKSMV ++M+++G FN GDF P L  LD+QGVA +MKK
Sbjct: 185 RVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKK 244

Query: 256 VHRWFDDFLTEILEERRMGGDGGQHDDLLSTLL-FSQDGADDENEKLNDTEIKALLLNMF 315
           +H  FD FLT+ILEE + G   G+  DLLSTL+    D AD++  KL DTEIKALLLN+F
Sbjct: 245 LHARFDAFLTDILEEHK-GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNLF 304

Query: 316 TAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKET 375
            AGTDTS+STVEWA+AELIR+PK+LAQAQ E+D VVGRDRLV +LDL QL YL+A+VKET
Sbjct: 305 VAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKET 364

Query: 376 FRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPG 435
           FRLHP TPLSLPR+A++SC+INGY IPKGSTLL+++WAI RDPN WA PLEFRP+RFLPG
Sbjct: 365 FRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPG 424

Query: 436 GEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLN 495
           GEK  VDVRGN+FE IPFGAGRRIC GM LG+RMVQL+ ATL+H+F+W L +G  P+ LN
Sbjct: 425 GEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLN 484

Query: 496 MDEAFGLTLRRAQPLVMHPRPRLSPDVYQG 511
           M+EA+GLTL+RA PLV+HPRPRL    Y G
Sbjct: 485 MEEAYGLTLQRADPLVVHPRPRLEAQAYIG 512

BLAST of MC06g0226 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 6.7e-186
Identity = 320/507 (63.12%), Postives = 395/507 (77.91%), Query Frame = 0

Query: 12  TLASAVILYRVLFL--------RRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMA 71
           TL   ++L  VLFL        R    + RLPPGP PWPI+GNLPH+G  P H  L+AM 
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62

Query: 72  GKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPY 131
             YGP+++LRLG VDVVVAAS +VAEQFLK+HD NF SRPPN+GAK++AYNYQDLVFAPY
Sbjct: 63  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122

Query: 132 GPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANA 191
           G RWRLLRKIS+VHLFS KA++  + VR+EEV  L   + R G  P  LG L+ +C  NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182

Query: 192 LGRTMIGRRVFGG-ADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKV 251
           LGR MIGRR+FG  AD K + F+SMV ++M L+G FN GDF PSL+ LDLQGVAG+MK++
Sbjct: 183 LGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 242

Query: 252 HRWFDDFLTEILEERRMGGDGGQHDDLLSTLL-FSQDGADDENEKLNDTEIKALLLNMFT 311
           H+ FD FL+ IL+E  M G   +H D+LSTL+       D +   L DTEIKALLLNMFT
Sbjct: 243 HKRFDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFT 302

Query: 312 AGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETF 371
           AGTDTSASTV+WA+AELIRHP ++ +AQ ELD VVGRDR V++ D+ QL YLQAV+KE F
Sbjct: 303 AGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENF 362

Query: 372 RLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGG 431
           RLHPPTPLSLP +A++SC+INGY IPKGSTLL +IWAI RDP++W+ PL F+P+RFLPGG
Sbjct: 363 RLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGG 422

Query: 432 EKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNM 491
           EK  VDV+G++FE IPFGAGRRIC G++LGLR +Q LTATLV  FDW L  G++P+KLNM
Sbjct: 423 EKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNM 482

Query: 492 DEAFGLTLRRAQPLVMHPRPRLSPDVY 509
           +E++GLTL+RA PLV+HP+PRL+P+VY
Sbjct: 483 EESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of MC06g0226 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.7e-168
Identity = 304/511 (59.49%), Postives = 382/511 (74.76%), Query Frame = 0

Query: 4   FSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMA 63
           F  +  +  L S+ I+Y +L+ RRS +   LPPGP  WPI+GNLP +G  P H  L A++
Sbjct: 4   FFFLWISTLLLSSFIVY-LLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDALS 63

Query: 64  GKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPY 123
            +YGPL  LRLGSV+VVVA+S+AVA QFL+ HD NF +RPPN+GA+++AYNYQDLVFAPY
Sbjct: 64  KQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPY 123

Query: 124 GPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAG--GAPATLGSLLTICTA 183
           GPRWR+LRK+ +VHLFS KA+D  R VR+ EV  LV  + R    G    LG  L +C  
Sbjct: 124 GPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCAT 183

Query: 184 NALGRTMIGRRVFGGADPKV---ERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGE 243
           NAL R M+GRRVF   D ++   + FK MV ++M L+G FN GDF P L  LDLQGV G+
Sbjct: 184 NALARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGK 243

Query: 244 MKKVHRWFDDFLTEILEERRMGGDGGQHDDLLSTLLFSQDGADDENE-KLNDTEIKALLL 303
           MK++HR +D FL  ++EE +     G   DLLS L+  ++ AD E E KLN+T+IKALLL
Sbjct: 244 MKRLHRRYDAFLDRVIEENQANAKSG---DLLSVLIRLKE-ADAEGEIKLNNTDIKALLL 303

Query: 304 NMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVV 363
           N+FTAGTDTS+STVEW +AELIRHP +L + Q ELDSV+GRDRLV++ DLP L YLQAVV
Sbjct: 304 NLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVV 363

Query: 364 KETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRF 423
           KETFRLHP TPLSLPRMA++ C +NGY+IPK +TLLV++W+IGRD   W  PLEFRP RF
Sbjct: 364 KETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRF 423

Query: 424 LPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPD 483
           LPGGE+ HVDV+GN+FE IPFGAGRRIC G++LGLRMVQ +TAT+VH++DW L  G    
Sbjct: 424 LPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQ 483

Query: 484 KLNMDEAFGLTLRRAQPLVMHPRPRLSPDVY 509
           KL+M+EA+GLTL+RA PL++ P PRLS   Y
Sbjct: 484 KLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of MC06g0226 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.0e-161
Identity = 291/512 (56.84%), Postives = 369/512 (72.07%), Query Frame = 0

Query: 15  SAVILYRVLFLR------RSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYGP 74
           SA + Y V FLR       + +   LPPGP+ WP++GNLP +G  P H+ + A+A +YGP
Sbjct: 16  SAAVWYLVYFLRGGSGGDAARKRRPLPPGPRGWPVLGNLPQLGDKPHHT-MCALARQYGP 75

Query: 75  LIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRWR 134
           L  LR G  +VVVAASA VA QFL+ HD NF +RPPN+GA+++AYNYQDLVFAPYG RWR
Sbjct: 76  LFRLRFGCAEVVVAASAPVAAQFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWR 135

Query: 135 LLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRTM 194
            LRK+ A+HLFS KA+D  R VRE EV ++V  +AR   A   LG    +C  N L R  
Sbjct: 136 ALRKLCALHLFSAKALDDLRAVREGEVALMVRNLARQQAASVALGQEANVCATNTLARAT 195

Query: 195 IGRRVF---GGADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVHRW 254
           IG RVF   GG   +   FK MV ++M L+G FN GDF P+L  LD QGV  +MK++HR 
Sbjct: 196 IGHRVFAVDGGEGAR--EFKEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRR 255

Query: 255 FDDFLTEILEERRMGG--DG---GQH-DDLLSTLLF---SQDGADDENEKLNDTEIKALL 314
           +D+ +   + ER+ G   DG   G+H +DLLS LL     +   D + EK+ +T+IKALL
Sbjct: 256 YDNMMNGFINERKAGAQPDGVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALL 315

Query: 315 LNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAV 374
           LN+FTAGTDT++STVEWA+AELIRHP +L +AQ ELD+VVGR RLVS+ DLP+L YL AV
Sbjct: 316 LNLFTAGTDTTSSTVEWALAELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAV 375

Query: 375 VKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDR 434
           +KETFRLHP TPLSLPR AA+ C+++GY+IPKG+TLLV++WAI RDP +W  PL+++P R
Sbjct: 376 IKETFRLHPSTPLSLPREAAEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSR 435

Query: 435 FLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSP 494
           FLPG     VDV+G +F  IPFGAGRRIC G++ GLRMV L+TATLVH FDW L NG +P
Sbjct: 436 FLPGRMHADVDVKGADFGLIPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATP 495

Query: 495 DKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVY 509
           DKLNM+EA+GLTL+RA PL++ P PRL P  Y
Sbjct: 496 DKLNMEEAYGLTLQRAVPLMVQPVPRLLPSAY 524

BLAST of MC06g0226 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 277/522 (53.07%), Postives = 373/522 (71.46%), Query Frame = 0

Query: 1   MEYFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLA 60
           ++    ++A V L  ++  Y +    +    L+LPPGPK +P++GNLP +G  P H  L 
Sbjct: 6   LDIILFISAIVFL--SIYYYNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKP-HQALQ 65

Query: 61  AMAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVF 120
           A +  YGPL+ LRLGSVD+VVA+S +VA QFLK +D+NF +RPPN+GA+++A+NY DLVF
Sbjct: 66  AFSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDLVF 125

Query: 121 APYGPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAV--ARAGGAPATLGSLLTI 180
           APYGPRWRLLRK+SAVHL   KA+D ++ VREEE+ VL   +     GG P  +G  + +
Sbjct: 126 APYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGEPVNVGKEMHV 185

Query: 181 CTANALGRTMIGRRVF-------GGAD-----PKVERFKSMVEQVMLLSGKFNAGDFFPS 240
           C+ NAL R M+GRRVF       GG +      K E FK MV +VM L+G FN GDF P 
Sbjct: 186 CSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFVPW 245

Query: 241 LERLDLQGVAGEMKKVHRWFDDFLTEILEERRMGGDGGQHDDLLSTLLFSQDGADDENEK 300
           L+  D+QGV  +MK+VHR ++ FL + + E R     G  +DLLS L+  +  +D    +
Sbjct: 246 LKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRSSAKPGA-NDLLSVLIGQRGKSDGSGGE 305

Query: 301 LNDTEIKALLLNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLD 360
           + DT IKAL+LN+ TAGTDTS+ST+EWA+ ELIRHP +L +AQ E+DS VGRDRLV++ D
Sbjct: 306 ITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRDRLVTESD 365

Query: 361 LPQLGYLQAVVKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEW 420
           +P+L YLQA+VKE FR+HP TPLSLPRM+ + C I GY IPK STL V+IWA+GRDP+ W
Sbjct: 366 VPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAMGRDPSIW 425

Query: 421 AGPLEFRPDRFLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSF 480
             P+EFRP RFLPGG+  H++VRGN+FE +PFGAGRRIC G ++G+R+V    ATL+H+F
Sbjct: 426 PDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTVATLIHAF 485

Query: 481 DWVLTNGLSPDKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVY 509
           DW L  GL+ +K++M+EAFG++L++A PL+ HP PRL+P  Y
Sbjct: 486 DWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of MC06g0226 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830 bits (2143), Expect = 6.82e-300
Identity = 412/512 (80.47%), Postives = 460/512 (89.84%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLAS V+LYR+LF +R+ RSL LPPGPKPWPIVGNLP IG APLHS LA M+G+YG
Sbjct: 9   SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLLADMSGEYG 68

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GSVDVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 69  PLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 128

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ HRRVREEEV VLV A+AR+G APATLGSLLT+CTANALGRT
Sbjct: 129 RLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVCTANALGRT 188

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN GDFFPSL+ LDL GVA EMKKVH
Sbjct: 189 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLGVAREMKKVH 248

Query: 248 RWFDDFLTEILEERRM------GGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLL 307
           +WFDD L++ILEE R       GG GG+H DLLSTLL ++D A+DENEKLNDTEIKALLL
Sbjct: 249 KWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLNDTEIKALLL 308

Query: 308 NMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVV 367
           NMFTAGTDTSASTVEWA+AELIRHPKM+ QAQ ELDS+VGRDR+VSDLDLP+LGYLQAVV
Sbjct: 309 NMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLPKLGYLQAVV 368

Query: 368 KETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRF 427
           KETFRLHPPTPLSLPRMAA+SC+I+GY+IPKGSTLLVD+WAI RDP EW  PLEFRPDRF
Sbjct: 369 KETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRF 428

Query: 428 LPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPD 487
           LPGGEK HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP 
Sbjct: 429 LPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDWALPSGLSPG 488

Query: 488 KLNMDEAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           +LNMDEAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 489 ELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of MC06g0226 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 825 bits (2130), Expect = 5.81e-298
Identity = 411/509 (80.75%), Postives = 456/509 (89.59%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLAS V+LYR+LF +R+ RSL LPPGPKPWPIVGNLP IG APLHS LA M+G+YG
Sbjct: 9   SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLLADMSGEYG 68

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GSVDVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 69  PLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 128

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ HRRVREEEV VLV A+AR+G APATLGSLLT+CTANALGRT
Sbjct: 129 RLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVCTANALGRT 188

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN GDFF  LE LDL GVAGEMKKVH
Sbjct: 189 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGVAGEMKKVH 248

Query: 248 RWFDDFLTEILEERR---MGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMF 307
           +WFDD +++ILEE R     G GG+H DLLSTLL ++D A DENEKLNDTEIKALLLNMF
Sbjct: 249 KWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTEIKALLLNMF 308

Query: 308 TAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKET 367
           TAGTDTSASTVEWA+AELIRHPKM+ QAQ ELDSVVGRDR+VSDLDLP+LGYLQAVVKET
Sbjct: 309 TAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKET 368

Query: 368 FRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPG 427
           FRLHPPTPLSLPRMAA+SC+I+GY IPKGSTLLVD+WAI RDP EW  PLEFRPDRFLPG
Sbjct: 369 FRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPG 428

Query: 428 GEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLN 487
           GEK HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP +LN
Sbjct: 429 GEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELN 488

Query: 488 MDEAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           MDEAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 489 MDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of MC06g0226 vs. NCBI nr
Match: XP_023007386.1 (flavonoid 3'-monooxygenase [Cucurbita maxima])

HSP 1 Score: 821 bits (2120), Expect = 1.79e-296
Identity = 408/507 (80.47%), Postives = 456/507 (89.94%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLA+ V+LYR+LF +R+ RSL LPPGPKPWP VGNLP IG APLHS LAAM+G+YG
Sbjct: 9   SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYG 68

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GS+DVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 69  PLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 128

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ +R VREEEV VLV A+AR G APATLGSLLT+CTANALGRT
Sbjct: 129 RLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRT 188

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN G+FFPSLE LDL GVA EMKKVH
Sbjct: 189 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVH 248

Query: 248 RWFDDFLTEILEERR-MGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMFTA 307
           +WFDD L++ILEE R +   GG+H DLLSTLL ++D A+DENEKLNDTEIKALLLNMFTA
Sbjct: 249 KWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKALLLNMFTA 308

Query: 308 GTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETFR 367
           GTDTSASTVEWA+AELIRHPKM+ QAQ ELDSVVGRDR+VSDLDLP+LGYLQAVVKETFR
Sbjct: 309 GTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFR 368

Query: 368 LHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGGE 427
           LHPPTPLSLPRMAA+SC+INGY IPKGSTLLVD+WAI RDP EW  PLEFRPDRFLPGGE
Sbjct: 369 LHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGE 428

Query: 428 KVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNMD 487
           K HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP +LNMD
Sbjct: 429 KAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMD 488

Query: 488 EAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           EAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 489 EAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of MC06g0226 vs. NCBI nr
Match: KAG6587576.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823 bits (2125), Expect = 9.70e-296
Identity = 410/509 (80.55%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLAS V+LYR+LF +R+ RSL LPPGPKPWPIVGNLP IG APLHS LA M+G+YG
Sbjct: 100 SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLLADMSGEYG 159

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GSVDVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 160 PLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 219

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ HRRVREEEV VLV A+AR G APATLGSLLT+CTANALGRT
Sbjct: 220 RLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRT 279

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN GDFFP LE LDL GVA EMKKVH
Sbjct: 280 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPWLEWLDLLGVAREMKKVH 339

Query: 248 RWFDDFLTEILEERR---MGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMF 307
           +WFDD +++ILEE R     G GG+H DLL+TLL ++D A DENEKLNDTEIKALLLNMF
Sbjct: 340 KWFDDLMSKILEEHRNLLATGGGGEHQDLLTTLLSTRDHAKDENEKLNDTEIKALLLNMF 399

Query: 308 TAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKET 367
           TAGTDTSASTVEWA+AELIRHPKM+ QAQ ELDSVVGRDR+VSDLDLP+LGYLQAVVKET
Sbjct: 400 TAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKET 459

Query: 368 FRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPG 427
           FRLHPPTPLSLPRMAA+SC+I+GY IPKGSTLLVD+WAI RDP EW  PLEFRPDRFLPG
Sbjct: 460 FRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPG 519

Query: 428 GEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLN 487
           GEK HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP +LN
Sbjct: 520 GEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELN 579

Query: 488 MDEAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           MDEAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 580 MDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 608

BLAST of MC06g0226 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 808 bits (2087), Expect = 2.37e-291
Identity = 406/522 (77.78%), Postives = 451/522 (86.40%), Query Frame = 0

Query: 2   EYFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAA 61
           E   +VAA +TLASA +L+R  FLRR FR   LPPGPKPWPIVGN+P IG APLHS LAA
Sbjct: 3   EITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLLAA 62

Query: 62  MAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFA 121
           MAGKYGPL+YLRLGSVD+VVAASAAVAEQ LK+HDTNFLSRPPNAGAKYIAYNYQD+VFA
Sbjct: 63  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 122

Query: 122 PYGPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTA 181
           PYGPRWRLLRKISAVHLFS+KA+D HRR+R++E+ VLV A+AR+G APA LGSLLT+C A
Sbjct: 123 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 182

Query: 182 NALGRTMIGRRVFGGA----DPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAG 241
           N+LGRTMIGRRVFG      D +  +FK+MVEQVM+L+GKFN GDFFP LERLDL GV  
Sbjct: 183 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 242

Query: 242 EMKKVHRWFDDFLTEILEERRM-------GGDGGQHDDLLSTLLFSQDGADDENEKLNDT 301
           EMKKVH+WFDDFLT+I+EE R        G  GG H DLLSTLL  +D   DENEKLNDT
Sbjct: 243 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 302

Query: 302 EIKALLLNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQL 361
           EIKALLLNMFTAGTDTSASTVEWA+ ELIRHP+M+AQAQ ELDSVVGRDR VSDLDLPQL
Sbjct: 303 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 362

Query: 362 GYLQAVVKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPL 421
            YLQAVVKETFRLHPPTPLSLPRMA+DSC++NGY IPKGSTLLVD+WAIGRDP EW  PL
Sbjct: 363 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 422

Query: 422 EFRPDRFLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVL 481
           EF P+RFLP GEK HVDV+GN+FEAIPFGAGRRICVG++LGLRMVQ+LTAT+VHSFDW L
Sbjct: 423 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 482

Query: 482 TNGLSPDKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVYQGLP 512
            NGL+PDKLNMDE +GLTLRRAQPLV+HPRPRL   VYQ LP
Sbjct: 483 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MC06g0226 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 825 bits (2130), Expect = 2.81e-298
Identity = 411/509 (80.75%), Postives = 456/509 (89.59%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLAS V+LYR+LF +R+ RSL LPPGPKPWPIVGNLP IG APLHS LA M+G+YG
Sbjct: 9   SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLLADMSGEYG 68

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GSVDVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 69  PLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 128

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ HRRVREEEV VLV A+AR+G APATLGSLLT+CTANALGRT
Sbjct: 129 RLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVCTANALGRT 188

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN GDFF  LE LDL GVAGEMKKVH
Sbjct: 189 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGVAGEMKKVH 248

Query: 248 RWFDDFLTEILEERR---MGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMF 307
           +WFDD +++ILEE R     G GG+H DLLSTLL ++D A DENEKLNDTEIKALLLNMF
Sbjct: 249 KWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTEIKALLLNMF 308

Query: 308 TAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKET 367
           TAGTDTSASTVEWA+AELIRHPKM+ QAQ ELDSVVGRDR+VSDLDLP+LGYLQAVVKET
Sbjct: 309 TAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKET 368

Query: 368 FRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPG 427
           FRLHPPTPLSLPRMAA+SC+I+GY IPKGSTLLVD+WAI RDP EW  PLEFRPDRFLPG
Sbjct: 369 FRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPG 428

Query: 428 GEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLN 487
           GEK HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP +LN
Sbjct: 429 GEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELN 488

Query: 488 MDEAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           MDEAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 489 MDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of MC06g0226 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 821 bits (2120), Expect = 8.69e-297
Identity = 408/507 (80.47%), Postives = 456/507 (89.94%), Query Frame = 0

Query: 8   AAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYG 67
           +AA+TLA+ V+LYR+LF +R+ RSL LPPGPKPWP VGNLP IG APLHS LAAM+G+YG
Sbjct: 9   SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYG 68

Query: 68  PLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRW 127
           PL+YLR GS+DVVVAASAA+AEQFLK+ DTNFLSRPPNAGAKYIAYNYQD+VFAPYGPRW
Sbjct: 69  PLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRW 128

Query: 128 RLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANALGRT 187
           RLLRKISAVHLFS+KA++ +R VREEEV VLV A+AR G APATLGSLLT+CTANALGRT
Sbjct: 129 RLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRT 188

Query: 188 MIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVH 247
           MIGRRVFG    G DP  ++FK+MVEQVM+LSGKFN G+FFPSLE LDL GVA EMKKVH
Sbjct: 189 MIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVH 248

Query: 248 RWFDDFLTEILEERR-MGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMFTA 307
           +WFDD L++ILEE R +   GG+H DLLSTLL ++D A+DENEKLNDTEIKALLLNMFTA
Sbjct: 249 KWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKALLLNMFTA 308

Query: 308 GTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETFR 367
           GTDTSASTVEWA+AELIRHPKM+ QAQ ELDSVVGRDR+VSDLDLP+LGYLQAVVKETFR
Sbjct: 309 GTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFR 368

Query: 368 LHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGGE 427
           LHPPTPLSLPRMAA+SC+INGY IPKGSTLLVD+WAI RDP EW  PLEFRPDRFLPGGE
Sbjct: 369 LHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGE 428

Query: 428 KVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNMD 487
           K HVDV+GN+F  IPFGAGRRICVGM++GLRMVQ+LTA+LVHSFDW L +GLSP +LNMD
Sbjct: 429 KAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMD 488

Query: 488 EAFGLTLRRAQPLVMHPRPRLSPDVYQ 509
           EAFGLTLRRAQPLV HPRPRLSP VYQ
Sbjct: 489 EAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of MC06g0226 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.15e-291
Identity = 406/522 (77.78%), Postives = 451/522 (86.40%), Query Frame = 0

Query: 2   EYFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAA 61
           E   +VAA +TLASA +L+R  FLRR FR   LPPGPKPWPIVGN+P IG APLHS LAA
Sbjct: 3   EITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLLAA 62

Query: 62  MAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFA 121
           MAGKYGPL+YLRLGSVD+VVAASAAVAEQ LK+HDTNFLSRPPNAGAKYIAYNYQD+VFA
Sbjct: 63  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 122

Query: 122 PYGPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTA 181
           PYGPRWRLLRKISAVHLFS+KA+D HRR+R++E+ VLV A+AR+G APA LGSLLT+C A
Sbjct: 123 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 182

Query: 182 NALGRTMIGRRVFGGA----DPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAG 241
           N+LGRTMIGRRVFG      D +  +FK+MVEQVM+L+GKFN GDFFP LERLDL GV  
Sbjct: 183 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 242

Query: 242 EMKKVHRWFDDFLTEILEERRM-------GGDGGQHDDLLSTLLFSQDGADDENEKLNDT 301
           EMKKVH+WFDDFLT+I+EE R        G  GG H DLLSTLL  +D   DENEKLNDT
Sbjct: 243 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 302

Query: 302 EIKALLLNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQL 361
           EIKALLLNMFTAGTDTSASTVEWA+ ELIRHP+M+AQAQ ELDSVVGRDR VSDLDLPQL
Sbjct: 303 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 362

Query: 362 GYLQAVVKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPL 421
            YLQAVVKETFRLHPPTPLSLPRMA+DSC++NGY IPKGSTLLVD+WAIGRDP EW  PL
Sbjct: 363 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 422

Query: 422 EFRPDRFLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVL 481
           EF P+RFLP GEK HVDV+GN+FEAIPFGAGRRICVG++LGLRMVQ+LTAT+VHSFDW L
Sbjct: 423 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 482

Query: 482 TNGLSPDKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVYQGLP 512
            NGL+PDKLNMDE +GLTLRRAQPLV+HPRPRL   VYQ LP
Sbjct: 483 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MC06g0226 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.15e-291
Identity = 406/522 (77.78%), Postives = 451/522 (86.40%), Query Frame = 0

Query: 2   EYFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAA 61
           E   +VAA +TLASA +L+R  FLRR FR   LPPGPKPWPIVGN+P IG APLHS LAA
Sbjct: 3   EITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLLAA 62

Query: 62  MAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFA 121
           MAGKYGPL+YLRLGSVD+VVAASAAVAEQ LK+HDTNFLSRPPNAGAKYIAYNYQD+VFA
Sbjct: 63  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 122

Query: 122 PYGPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTA 181
           PYGPRWRLLRKISAVHLFS+KA+D HRR+R++E+ VLV A+AR+G APA LGSLLT+C A
Sbjct: 123 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 182

Query: 182 NALGRTMIGRRVFGGA----DPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAG 241
           N+LGRTMIGRRVFG      D +  +FK+MVEQVM+L+GKFN GDFFP LERLDL GV  
Sbjct: 183 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 242

Query: 242 EMKKVHRWFDDFLTEILEERRM-------GGDGGQHDDLLSTLLFSQDGADDENEKLNDT 301
           EMKKVH+WFDDFLT+I+EE R        G  GG H DLLSTLL  +D   DENEKLNDT
Sbjct: 243 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 302

Query: 302 EIKALLLNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQL 361
           EIKALLLNMFTAGTDTSASTVEWA+ ELIRHP+M+AQAQ ELDSVVGRDR VSDLDLPQL
Sbjct: 303 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 362

Query: 362 GYLQAVVKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPL 421
            YLQAVVKETFRLHPPTPLSLPRMA+DSC++NGY IPKGSTLLVD+WAIGRDP EW  PL
Sbjct: 363 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 422

Query: 422 EFRPDRFLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVL 481
           EF P+RFLP GEK HVDV+GN+FEAIPFGAGRRICVG++LGLRMVQ+LTAT+VHSFDW L
Sbjct: 423 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 482

Query: 482 TNGLSPDKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVYQGLP 512
            NGL+PDKLNMDE +GLTLRRAQPLV+HPRPRL   VYQ LP
Sbjct: 483 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MC06g0226 vs. ExPASy TrEMBL
Match: A0A5N6RRZ1 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_019316 PE=3 SV=1)

HSP 1 Score: 719 bits (1857), Expect = 7.00e-257
Identity = 362/512 (70.70%), Postives = 419/512 (81.84%), Query Frame = 0

Query: 1   MEYFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLA 60
           M  FSL+    T+A A+ LY +L LR      RLPPGPKPWPI+GNLPH+G  P HS LA
Sbjct: 1   MALFSLILG--TIALAIFLYYLLNLRTHRHRHRLPPGPKPWPIIGNLPHMGTVPHHS-LA 60

Query: 61  AMAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVF 120
           AMA  YGPL++LRLG VDVVVAASA+VA QFLK HD NF SRPPN+GAKYIAYNYQDLVF
Sbjct: 61  AMARTYGPLMHLRLGFVDVVVAASASVASQFLKTHDANFSSRPPNSGAKYIAYNYQDLVF 120

Query: 121 APYGPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICT 180
           APYG RWR+LRKIS+VHLFS KA+D  R VR+EEV VL  A+  AG  P  L  LL +CT
Sbjct: 121 APYGERWRMLRKISSVHLFSGKALDEFRHVRQEEVAVLARALVNAGKKPVNLAQLLNVCT 180

Query: 181 ANALGRTMIGRRVFG----GADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVA 240
            NALGR M+GRRVFG    G D K + FKSMV +VM+L+G FN GDF P+LE LDLQGVA
Sbjct: 181 VNALGRVMLGRRVFGDGSGGGDAKADEFKSMVVEVMVLAGVFNIGDFIPALEWLDLQGVA 240

Query: 241 GEMKKVHRWFDDFLTEILEERRMGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALL 300
            +MKK+H  FD FLT I+E+ +  G GGQH DLLSTL+  ++ AD E  KL DTEIKALL
Sbjct: 241 AKMKKLHNRFDAFLTGIVEDHKKNG-GGQHMDLLSTLISLKENADGEGGKLTDTEIKALL 300

Query: 301 LNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAV 360
           LNMFTAGTDTS+STVEWA+AELIRHPK+LAQ Q ELDSVVGR+RLVS+LDLPQL YLQAV
Sbjct: 301 LNMFTAGTDTSSSTVEWAIAELIRHPKILAQVQQELDSVVGRERLVSELDLPQLTYLQAV 360

Query: 361 VKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDR 420
           +KETFRLHP TPLSLPRMAA+SC+INGY IPKGSTLLV++WAI RDP++W+ PLEF+P+R
Sbjct: 361 IKETFRLHPSTPLSLPRMAAESCEINGYHIPKGSTLLVNVWAIARDPDQWSDPLEFKPER 420

Query: 421 FLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSP 480
           FLPG EK +VDVRGN+FE IPFGAGRRIC GM+LGLRMV LLTA+L+H+FDW L N L P
Sbjct: 421 FLPGSEKANVDVRGNDFEVIPFGAGRRICAGMSLGLRMVHLLTASLIHAFDWNLANELMP 480

Query: 481 DKLNMDEAFGLTLRRAQPLVMHPRPRLSPDVY 508
           +KLNMDEA+GLTL+RA+PL++HPRPRLSP VY
Sbjct: 481 EKLNMDEAYGLTLQRAEPLIVHPRPRLSPHVY 508

BLAST of MC06g0226 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 651.7 bits (1680), Expect = 4.8e-187
Identity = 320/507 (63.12%), Postives = 395/507 (77.91%), Query Frame = 0

Query: 12  TLASAVILYRVLFL--------RRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMA 71
           TL   ++L  VLFL        R    + RLPPGP PWPI+GNLPH+G  P H  L+AM 
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62

Query: 72  GKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPY 131
             YGP+++LRLG VDVVVAAS +VAEQFLK+HD NF SRPPN+GAK++AYNYQDLVFAPY
Sbjct: 63  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122

Query: 132 GPRWRLLRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAGGAPATLGSLLTICTANA 191
           G RWRLLRKIS+VHLFS KA++  + VR+EEV  L   + R G  P  LG L+ +C  NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182

Query: 192 LGRTMIGRRVFGG-ADPKVERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKV 251
           LGR MIGRR+FG  AD K + F+SMV ++M L+G FN GDF PSL+ LDLQGVAG+MK++
Sbjct: 183 LGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 242

Query: 252 HRWFDDFLTEILEERRMGGDGGQHDDLLSTLL-FSQDGADDENEKLNDTEIKALLLNMFT 311
           H+ FD FL+ IL+E  M G   +H D+LSTL+       D +   L DTEIKALLLNMFT
Sbjct: 243 HKRFDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFT 302

Query: 312 AGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETF 371
           AGTDTSASTV+WA+AELIRHP ++ +AQ ELD VVGRDR V++ D+ QL YLQAV+KE F
Sbjct: 303 AGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENF 362

Query: 372 RLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGG 431
           RLHPPTPLSLP +A++SC+INGY IPKGSTLL +IWAI RDP++W+ PL F+P+RFLPGG
Sbjct: 363 RLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGG 422

Query: 432 EKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNM 491
           EK  VDV+G++FE IPFGAGRRIC G++LGLR +Q LTATLV  FDW L  G++P+KLNM
Sbjct: 423 EKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNM 482

Query: 492 DEAFGLTLRRAQPLVMHPRPRLSPDVY 509
           +E++GLTL+RA PLV+HP+PRL+P+VY
Sbjct: 483 EESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of MC06g0226 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 341.3 bits (874), Expect = 1.4e-93
Identity = 196/517 (37.91%), Postives = 292/517 (56.48%), Query Frame = 0

Query: 2   EYFSLVAAAVTLASAVILYRVLFLRRSFRSLR---LPPGPKPWPIVGNLPHIGRAPLHSH 61
           E +S V  AV    AV+ Y  L+++   +S +   LPPGP P PIVGNLP +    LH+ 
Sbjct: 20  EPYSSVMLAVAALLAVVCY--LWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQ 79

Query: 62  LAAMAGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDL 121
             A+  KYGPL+ + LGS   +V +S  +A + LK HD  F +       K   Y  +D+
Sbjct: 80  FQALTLKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDI 139

Query: 122 VFAPYGPRWRLLRKISAVHLFSTKAMDAHRRVREEE---VVVLVGAVARAGGAPATLGSL 181
           +++PYG  WR LRK+  + +F+T +++A    R EE    +V +  +AR  G+P  LG  
Sbjct: 140 LWSPYGTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMARE-GSPVNLGEQ 199

Query: 182 LTICTANALGRTMIGRRVFGGADPKV-ERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQG 241
           + +   N + R M G  V G     +    K+++  +  + G  N  DFFP   R D QG
Sbjct: 200 IFLSIFNVVTRMMWGATVEGDERTSLGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQG 259

Query: 242 VAGEMKKVHRWFDDFLTEILEE--RRMGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEI 301
           +  +MK   +  D     ++E   + +G    + +D L  LL  +D  DDE   L+ T +
Sbjct: 260 LVKQMKGHVKKLDLLFDRVMESHVKMVGKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHV 319

Query: 302 KALLLNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGY 361
           K+LL++M   G DTS +  E+AMAE++  P++L + +LELD VVG+D +V +  LP+L Y
Sbjct: 320 KSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPY 379

Query: 362 LQAVVKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEF 421
           LQAV+KET RLHP  PL +P   +++  + GY +PK S + +++WAI RDP  W  P EF
Sbjct: 380 LQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEF 439

Query: 422 RPDRFLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTN 481
           +P+RFL       +D  G +F+ +PFG+GRRIC  + +  R+V    A+L+HSFDW    
Sbjct: 440 KPERFLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQ 499

Query: 482 GLSPDKLNMDEAFGLTLRRAQPLVMHPRPRLS-PDVY 509
           G    K  ++E FGL L+   PLV  P PRLS P +Y
Sbjct: 500 G---QKFEVEEKFGLVLKLKSPLVAIPVPRLSDPKLY 524

BLAST of MC06g0226 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-92
Identity = 190/502 (37.85%), Postives = 280/502 (55.78%), Query Frame = 0

Query: 20  YRVLFLRRSFRSL----------RLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYGPL 79
           Y VL L  +F  L           LPPGP+  PIVGNLP +    LH++   +A  +GP+
Sbjct: 20  YAVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLD-PDLHTYFTKLAQSHGPI 79

Query: 80  IYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRWRL 139
             L LGS   VV  S ++A + LK  D NF +      A+ + Y   DLV+ PYG  WR+
Sbjct: 80  FKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRM 139

Query: 140 LRKISAVHLFSTKAMDAHRRVREEEVVVLVGAVARAG--GAPATLGSLLTICTANALGRT 199
           LRK+ A  LFS K +D+   +R +E+      + + G   +P  +G  L +   N +   
Sbjct: 140 LRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNM 199

Query: 200 MIGRRVFGGADPKV-ERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGEMKKVHRWF 259
           + G  V       V   FK ++ ++  L G  N  DFFP L R DLQG+  +M    R  
Sbjct: 200 LWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDL 259

Query: 260 DDFLTEILEE----RRMGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMFTA 319
           D  L   +E+    R   GD G+  D L  L+  +D   D +  +    +KA+L++M   
Sbjct: 260 DAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMVVG 319

Query: 320 GTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETFR 379
           GT++S +T+E+ MAELI +P+++ +AQ ELD VVG+D +V +  +  L Y+ AV+KET R
Sbjct: 320 GTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKETLR 379

Query: 380 LHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGGE 439
           L+P  PL +P   +++  + GY IPK + + +++W+I RDPN W  P EFRP+RFL   +
Sbjct: 380 LYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL---D 439

Query: 440 KVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNMD 499
           K   D  G ++  +PFG+GRRIC G+AL  RM+    ATL+HSFDW + +G     L+++
Sbjct: 440 KKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDG---HVLDLE 499

Query: 500 EAFGLTLRRAQPLVMHPRPRLS 505
           E FG+ L+   PLV  P PRLS
Sbjct: 500 EKFGIVLKLKTPLVALPIPRLS 514

BLAST of MC06g0226 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 337.0 bits (863), Expect = 2.6e-92
Identity = 190/513 (37.04%), Postives = 285/513 (55.56%), Query Frame = 0

Query: 3   YFSLVAAAVTLASAVILYRVLFLRRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAM 62
           Y S++     L + V  + +    +S     LPPGP P PIVGNLP +    LH+   A+
Sbjct: 53  YSSVMLGVAALLAVVCYFWIQGKSKSKNGPPLPPGPWPLPIVGNLPFLNSDVLHTQFQAL 112

Query: 63  AGKYGPLIYLRLGSVDVVVAASAAVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAP 122
             K+GPL+ + LGS   +V +S  +A + LK HD  F +       K   Y  +D++++P
Sbjct: 113 TLKHGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSP 172

Query: 123 YGPRWRLLRKISAVHLFSTKAMDAHRRVREEE---VVVLVGAVARAGGAPATLGSLLTIC 182
           YG  WR LRK+  + +F+T  ++A    R EE    +V +  +AR  G+P  LG  + + 
Sbjct: 173 YGTHWRRLRKLCVMKMFTTPTLEASYSTRREETRQTIVHMSEMARE-GSPVNLGEQIFLS 232

Query: 183 TANALGRTMIGRRVFGGADPKV-ERFKSMVEQVMLLSGKFNAGDFFPSLERLDLQGVAGE 242
             N + R M G  V G     +    K+++  +  + G  N  DFFP   R D QG+  +
Sbjct: 233 IFNVVTRMMWGATVEGDERTSLGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVKQ 292

Query: 243 MKKVHRWFDDFLTEILEE--RRMGGDGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALL 302
           MK   +  D     ++E   + +G    + +D L  LL  +D  DDE   L+ T +K+LL
Sbjct: 293 MKGHVKKLDLLFDRVMESHVKMVGKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHVKSLL 352

Query: 303 LNMFTAGTDTSASTVEWAMAELIRHPKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAV 362
           ++M   G DTS +  E+AMAE++  P++L + +LELD VVG+D +V +  LP+L YLQAV
Sbjct: 353 MDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQAV 412

Query: 363 VKETFRLHPPTPLSLPRMAADSCQINGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDR 422
           +KET RLHP  PL +P   +++  + GY +PK S + +++WAI RDP  W  P EF+P+R
Sbjct: 413 MKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKPER 472

Query: 423 FLPGGEKVHVDVRGNNFEAIPFGAGRRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSP 482
           FL       +D  G +F+ +PFG+GRRIC  + +  R+V    A+L+HSFDW    G   
Sbjct: 473 FLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQG--- 532

Query: 483 DKLNMDEAFGLTLRRAQPLVMHPRPRLS-PDVY 509
            K  ++E FGL L+   PLV  P PRLS P +Y
Sbjct: 533 QKFEVEEKFGLVLKLKSPLVAIPVPRLSDPKLY 555

BLAST of MC06g0226 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 335.5 bits (859), Expect = 7.6e-92
Identity = 180/484 (37.19%), Postives = 283/484 (58.47%), Query Frame = 0

Query: 26  RRSFRSLRLPPGPKPWPIVGNLPHIGRAPLHSHLAAMAGKYGPLIYLRLGSVDVVVAASA 85
           RR+++  R PP P   PI+GNL  +G  P H  L  ++ KYGP++ L+LG V  V+ +S+
Sbjct: 25  RRNYQ--RTPPSPPGCPIIGNLHQLGELP-HQSLWKLSKKYGPVMLLKLGRVPTVIVSSS 84

Query: 86  AVAEQFLKLHDTNFLSRPPNAGAKYIAYNYQDLVFAPYGPRWRLLRKISAVHLFSTKAMD 145
             A+Q LK+HD +  SRP  AGA+ ++YNY D+ F+PY   W+ +RK++   LFS+K + 
Sbjct: 85  ETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEVRKLAVQELFSSKQVH 144

Query: 146 AHRRVREEEVVVLVGAVARAGG--APATLGSLLTICTANALGRTMIGRRVFGGADPKVER 205
           + + +++EEV  L+ +++ +     P  L   L   T + + RT      F G     ER
Sbjct: 145 SIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTAFSVN-FEGTVLNSER 204

Query: 206 FKSMVEQVMLLSGKFNAGDFFPSLERLD--LQGVAGEMKKVHRWFDDFLTEILEERRMGG 265
           F ++V + + + G F+A DF P + R+   L G+ G  ++  R  D F  ++ +  +   
Sbjct: 205 FNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDLDAFYEQMFDLHKQKK 264

Query: 266 DGGQHDDLLSTLLFSQDGADDENEKLNDTEIKALLLNMFTAGTDTSASTVEWAMAELIRH 325
           + G  D +   L   ++ A   N+KL    IKA+L+++  AG DTSA T+ WAMAEL ++
Sbjct: 265 EEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAELAKN 324

Query: 326 PKMLAQAQLELDSVVGRDRLVSDLDLPQLGYLQAVVKETFRLHPPTPLSLPRMAADSCQI 385
           P+++ + Q E+ S +     +S  D  +L YL+ V+KET+RLHP TPL +PR A    +I
Sbjct: 325 PRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPREAMSEFEI 384

Query: 386 NGYQIPKGSTLLVDIWAIGRDPNEWAGPLEFRPDRFLPGGEKVHVDVRGNNFEAIPFGAG 445
           NGY IP  + L V++WAIGRDP+ W  P  F P+RF       ++D +G +FE +PFG G
Sbjct: 385 NGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDN----NIDAKGQHFELLPFGGG 444

Query: 446 RRICVGMALGLRMVQLLTATLVHSFDWVLTNGLSPDKLNMDEAFGLTLRRAQPLVMHPRP 505
           RR+C  + +G  MV+   A L++ FDW L  G+  D ++M+EA GLT+ +   L++ P  
Sbjct: 445 RRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELILVPTK 500