MC06g0221 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0221
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionN-acetylglucosaminylphosphatidylinositol deacetylase
LocationMC06: 1850566 .. 1855149 (+)
RNA-Seq ExpressionMC06g0221
SyntenyMC06g0221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAATGCTCGTATGGCTTCCTCTAATTCGTCCATATGAATCCTGTCGCCTTCTCGATCCCACACTTTCCAGCTCCTGCAATTAGAAATTCCAAAATTTCTCCTGAAATCGTTGAGAAATTCTCATCTGTAGTGACGGTAGTCGCAGAAAGCTCATGGTTCATGCTCTCTAATTTTCCCGGGAAGGAAGGGAAGGAAATAGTGGGTATATTCATCATAATGATGGTGGTTTAATGTCTTCTTTTTTACTTATTTTTTGCGGTGGGCATTTTGACGACTCTGATTTTACTCTCTTCTGTCTCCAATCAATTTTACGCGCCTGAAACAAAGTTGTTAAATCTGTTTTTGCCAATTGTGTGTTAATCTTCGTTTTCTTCTGTTATTTTACTCGGATGTGACTGGAATTTGTTGCTAATCAGCTGTATGTAGGCTACTGACTGTGAAATGTTTTGGCTTTTCAAGTTTAGAATTTGATGGAATTTTATCTTTTTTGGTTCTCTTTCTGCATTCTCCAGTTAATAACGAATAAAGTTGTCTGTAAATGTTTTCAGGGGAGTCCTAGATGGCTTGGCTGTTGGTTATTACTGCATTCGTTGTAATTTGGGTTGCTTCATTATGCAAAATTCTTCATATATCAGTCTCATCTTCTAGGAGTGCTTTCTTAAATAGTGGTGATTATGGATTATTTTGTTGAATTTTCTCTTTTGTTCTAAATTTCTTTCTTCTTCAAAACTAAAATTCTCCTATCTGTTATCCGTTGACATTTAGATGGAACTCAAAAGAGAAATGTCTTGCTGGTTGTTGCCCATCCGGATGATGAGTCTATGTAAGCATTTTAGATGTCTCCATATTTGTCAACTTTTCTTTACTTTTCTTGCTGGGTCTATATCTTGCTCTAATGAATTTGGTCATAGGTTCTATCTTATTGCCCACCGTATATTTTGAGTTCTACCTTCAAAAAAGAAAGTTCCTGCAGTTCTCTTTGCAGATAATTGGAATTGATAATGCTACTGTAATTACTTCTTCCATTTATACACTGGTTCTGGTTAAAACAGCAAATGTGATCAGAATTTGAGATGCTCATAGACCTAATATTCAACTATGACTTAAAATTTAATACGTGAACATTTGTTATAACAATAAAATAATGTTCATAAACATAAAATTCAAAATTAGTTAATATTTGTTGAAGTGCATTAAATCATTGGAATTGGCTCCATGTTTCCTGTATCGGGTTCATATGCCTTCCTTCTAATATTAATGTAAAAATGTTGTTGATGGTCTCGTTATGTAGCCCCCATGGGTTAGAAGTTTCTGCAGGTGAACTATCAACTTAACTTGGTCTGTTCACCATGTTGTGTCACAATGTGCAATTTTATTTTGGTTCTCTCTATTATTCAATGTGTATGTAATTGTCTCCCCTTTTATTTCACAGCTCCTGATGCCGTTCTTCTATCTCCCTGCAGGTTTTTTTCTCCAACAATAAACTTCCTAACATCAAGGGGGCATAATCTTCATATTCTGTGCATGTCAATTGGTATTTTAGAGAACCAGCCATCTAACACAACAAACTCAAAAAACAAGGGAAAGCAAATTCTGAAAAACAATTATTCAGACATATTTTCATTGAACTTTGTTCTAAAAAATAGAAAATTGTTTCTGAAAATAGCTTCCAAACAAGCACTTACTTTCTTTTCTTTCTTTATTTCTTTCTTTTTTTGAGTACTTAGTTTCTATTGTTATGTTCTTCGTTGATTGTTTAGGTAATGCAGATGAGATGGGAACCATCAGAAGAGAAGAACTATACAAAGCTTCCACAATTCTTAAGGTGTTTTGTGCCTTAAAATAGAAGTTTTTAATTTCTGTTATCTAGAATACTGGCTTTCTTCTCCATGAAAGTTGGTCCTCTTTCCCCCCCCCCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTCCCCCCCCCCCCCCAAAAAAAAAAAAAATAATAATAATAATAATAATAAACACGAAGAAGAAGGAGAAGATTAGATTCTTTGTTGTCGCGTTTTGCCTAAAACTTTTTGAAAAATTTTTCCCCTATTTGGATTGCTTTGGGATATAGACGAGGTTAAAAGTTGCTTGTCATAAACCCTCAAATCTTGTGCACGCTTAGGTTTCTGGTTCTATATTTAGTCTGCTTTTGTTGTTCAAATCTAGGTTCCCCTCAATCAAGTGAAGATCCTCGACCATCCAGATTTGCAGGTTTGACTAAAAAGTGTCTGGTTCAGTTTAATTCCACTGTCAATATTTGAGATAGCATGTGATGAATCCAAGCCAATATGGTGATGCCATCAGGATGGTTTTGGCAAAGTGTGGAATCATAAATTGCTGGCAAAGATTATTGAACAGGAGATATCAAGCCATGGGATTGACTCGGTAATTTATTGGATTTATTTAACTTGGTTACCGGCGCAATCTTGATATTATTTCTGTGTTCAGCTTTGGTTCTGAATTTTATTTCATTTTCTATCTAACACGTCCTAAGTTCTGTTCTTTCACTTTTCATGGCTTTTAACAGATTATTACCTTTGATAGCTATGGAGTTTCTGGTCACTGTAATCACCGTGATGTGCACTATGGGGTGCAGTAAGCTAGCTTCTTAATTTTGTAAATTGGATATTTTTTAACGTCAAAACTTTTCATACTTAGTCCATTTATCGAAATTGGAGAATTATATACTTGAGGGTTCTATCAAATGACAGTAACTAAAAGTTGCTCTCTCCACAATCTTTATTCTTTATCCTAAACCATTCCAACCTTTTGATTGATTGCTTCTAAGTCTACGTATTTTGTGAACCCAAACCCTGTTCTCTACTCTCTGCACTGTAATTTAGAATCACTTTTAAACTAGCAAATTTTTTTTTTCTTTTGATGAAGACTTAACTTACGTTTCTGTCTGCTCTGTTTGCTGAATCCCTAATGGAAAATAGTTTATTCATTCTTGCTTCTGTTTTAACAGTTTTTATATATATGTATATTAGTTGTAGGTTGTAGCTGGACAGTTTATTTATTTCTCAGAAGAAAATGTTACCTTGCCCATGCTTGACTGCTATAATGAAGTAATCAGTCATGGGATATTATCCAAAAATTGAGAGTTGAAGATGAATGTAATGTTTATGTCTTTATATTTATATTAGTGTACCTTATTGTAAATTAAAAGAGAGCTCTCACTATCAACTCCATGCCTAGTGTAATCATGATGTATTTCTCCTTCTCTCTAGTAACAAATCTTTAACTTTTTTTTTCCAATGCTGTAAACAGAAGTTTCTTGCTTAATAAAGTTCCACAAAGTTTTGAGGCCTGGGAACTTGTAAGTTTTCCTTCTCTACATACTCAATTCTCCTTCAGTCACTTTTTAAGGTTGGCTAACCTTCGAGTTTAGAATCCCTCATTTGCGGGATTTTTATTCTTCCTAACTTAAGTTTCCATTTCCACTAGTAAATGCTTTAAAGTTCAGGGTTCAGTTATTATATCTATTTTTTTATGCTTAATAGCAGACAAAAGATATTTTAAGCAAATCATGAGTAATTCTTGCCTAATCTAGTCAGTAGGGATTTTGGTGTCTATTATAACATGTTTGTGTGTCTATGACCCATTTATCTTCAGGTCTTAGGTGTTCCAGTTTATATTTAATTGGTACTTTGAACCATGACCTAACTTTTCCCTGTTTATTCTTATTCCCAGGTCAGTACCAATATACTGCGCAAGTATAGTGGACCATTCGACATTTGGTTATCTATGTTATCCAAAGAGAATACAAGTGGAATGATGCATTGCTTATTAAATGAATGCCCCCAGAAAAGCTTCCTCGCAATGGCTCAGCACTCAAGCCAATGGGTTTGGTATGTATGTGTAGTGATCTTTATTTGAGAGCCAGAACACGAGTTCTCTCATTTCATTTTGAATACCATGTTTCACTTGGTTCCATTAGCAATGAAATCGGGTTTTGGGTGGCTGGGAAAATCCTTTACCCGTCTTCTAATTGCCCATCTTCGATCTGGCAGGTTCCGCAAGCTCTTTGTTACCTTTTCCAGTTATACCTATGTCAACACACTCAAAAGAATTAATTAGCCGTGCTTGCTGTTATAATGCCACAAGATGTTACCAACTTTGCAGATATCTACTTCGGGGGAACTGTGAACTCGGATGGCTTGATTTCATGTAAGGTTTCAATTGGAGTCTCTCCCCTTTTTCTGGTGCGTTTGAATGATCGTTCTTTCTTTTTATATTACAACAAAAGTGGGGTGTGGAGATTTTAATCTCCGGCTTAAAAAAGAGTAGATATCATTTACATATGTGCTATGTTCATGTTGCAATTTTAATGATTTTGCTTTCGATGTATAAAACTTCACCACACTGATGTTACTCATCTTTAGAAACTAAACTTAGGCCCCATTTATTCATTGACTTCAACTTGTTTGTTAGAAATCCACAGACCGAATTGGGAATGCGGGATTGACAATTCCAGTTGAGAAATGTATAGTTTATAGTTTTATACCAG

mRNA sequence

AAGAAATGCTCGTATGGCTTCCTCTAATTCGTCCATATGAATCCTGTCGCCTTCTCGATCCCACACTTTCCAGCTCCTGCAATTAGAAATTCCAAAATTTCTCCTGAAATCGTTGAGAAATTCTCATCTGTAGTGACGGTAGTCGCAGAAAGCTCATGGTTCATGCTCTCTAATTTTCCCGGGAAGGAAGGGAAGGAAATAGTGGGGGAGTCCTAGATGGCTTGGCTGTTGGTTATTACTGCATTCGTTGTAATTTGGGTTGCTTCATTATGCAAAATTCTTCATATATCAGTCTCATCTTCTAGGAGTGCTTTCTTAAATAGTGATGGAACTCAAAAGAGAAATGTCTTGCTGGTTGTTGCCCATCCGGATGATGAGTCTATGTTTTTTTCTCCAACAATAAACTTCCTAACATCAAGGGGGCATAATCTTCATATTCTGTGCATGTCAATTGGTAATGCAGATGAGATGGGAACCATCAGAAGAGAAGAACTATACAAAGCTTCCACAATTCTTAAGGTTCCCCTCAATCAAGTGAAGATCCTCGACCATCCAGATTTGCAGGATGGTTTTGGCAAAGTGTGGAATCATAAATTGCTGGCAAAGATTATTGAACAGGAGATATCAAGCCATGGGATTGACTCGATTATTACCTTTGATAGCTATGGAGTTTCTGGTCACTGTAATCACCGTGATGTGCACTATGGGGTGCAAAGTTTCTTGCTTAATAAAGTTCCACAAAGTTTTGAGGCCTGGGAACTTGTCAGTACCAATATACTGCGCAAGTATAGTGGACCATTCGACATTTGGTTATCTATGTTATCCAAAGAGAATACAAGTGGAATGATGCATTGCTTATTAAATGAATGCCCCCAGAAAAGCTTCCTCGCAATGGCTCAGCACTCAAGCCAATGGGTTTGGTTCCGCAAGCTCTTTGTTACCTTTTCCAGTTATACCTATGTCAACACACTCAAAAGAATTAATTAGCCGTGCTTGCTGTTATAATGCCACAAGATGTTACCAACTTTGCAGATATCTACTTCGGGGGAACTGTGAACTCGGATGGCTTGATTTCATGTAAGGTTTCAATTGGAGTCTCTCCCCTTTTTCTGGTGCGTTTGAATGATCGTTCTTTCTTTTTATATTACAACAAAAGTGGGGTGTGGAGATTTTAATCTCCGGCTTAAAAAAGAGTAGATATCATTTACATATGTGCTATGTTCATGTTGCAATTTTAATGATTTTGCTTTCGATGTATAAAACTTCACCACACTGATGTTACTCATCTTTAGAAACTAAACTTAGGCCCCATTTATTCATTGACTTCAACTTGTTTGTTAGAAATCCACAGACCGAATTGGGAATGCGGGATTGACAATTCCAGTTGAGAAATGTATAGTTTATAGTTTTATACCAG

Coding sequence (CDS)

ATGGCTTGGCTGTTGGTTATTACTGCATTCGTTGTAATTTGGGTTGCTTCATTATGCAAAATTCTTCATATATCAGTCTCATCTTCTAGGAGTGCTTTCTTAAATAGTGATGGAACTCAAAAGAGAAATGTCTTGCTGGTTGTTGCCCATCCGGATGATGAGTCTATGTTTTTTTCTCCAACAATAAACTTCCTAACATCAAGGGGGCATAATCTTCATATTCTGTGCATGTCAATTGGTAATGCAGATGAGATGGGAACCATCAGAAGAGAAGAACTATACAAAGCTTCCACAATTCTTAAGGTTCCCCTCAATCAAGTGAAGATCCTCGACCATCCAGATTTGCAGGATGGTTTTGGCAAAGTGTGGAATCATAAATTGCTGGCAAAGATTATTGAACAGGAGATATCAAGCCATGGGATTGACTCGATTATTACCTTTGATAGCTATGGAGTTTCTGGTCACTGTAATCACCGTGATGTGCACTATGGGGTGCAAAGTTTCTTGCTTAATAAAGTTCCACAAAGTTTTGAGGCCTGGGAACTTGTCAGTACCAATATACTGCGCAAGTATAGTGGACCATTCGACATTTGGTTATCTATGTTATCCAAAGAGAATACAAGTGGAATGATGCATTGCTTATTAAATGAATGCCCCCAGAAAAGCTTCCTCGCAATGGCTCAGCACTCAAGCCAATGGGTTTGGTTCCGCAAGCTCTTTGTTACCTTTTCCAGTTATACCTATGTCAACACACTCAAAAGAATTAATTAG

Protein sequence

MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN
Homology
BLAST of MC06g0221 vs. ExPASy Swiss-Prot
Match: Q9Y2B2 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Homo sapiens OX=9606 GN=PIGL PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.2e-36
Identity = 90/253 (35.57%), Postives = 144/253 (56.92%), Query Frame = 0

Query: 3   WLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGT--QKRNVLLVVAHPDDESMFFSP 62
           WLL +   V+ W       L +  SS R       G    +   LLV+AHPDDE+MFF+P
Sbjct: 5   WLLCVALAVLAW-----GFLWVWDSSERMKSREQGGRLGAESRTLLVIAHPDDEAMFFAP 64

Query: 63  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 122
           T+  L    H +++LC S GN    G  R++EL ++  +L +PL+ V I+D+ D  D  G
Sbjct: 65  TVLGLARLRHWVYLLCFSAGNYYNQGETRKKELLQSCDVLGIPLSSVMIIDNRDFPDDPG 124

Query: 123 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSF-LLNKVPQSFEA 182
             W+ + +A+++ Q I  +GI+ ++TFD+ GVSGH NH  ++  V++     K+P+    
Sbjct: 125 MQWDTEHVARVLLQHIEVNGINLVVTFDAGGVSGHSNHIALYAAVRALHSEGKLPKGCSV 184

Query: 183 WELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 242
             L S N+LRKY    D+ LS+L   +T  ++  L ++   ++  AM+ H SQ +WFR+L
Sbjct: 185 LTLQSVNVLRKYISLLDLPLSLL---HTQDVLFVLNSKEVAQAKKAMSCHRSQLLWFRRL 244

Query: 243 FVTFSSYTYVNTL 253
           ++ FS Y  +N+L
Sbjct: 245 YIIFSRYMRINSL 249

BLAST of MC06g0221 vs. ExPASy Swiss-Prot
Match: Q5SX19 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Mus musculus OX=10090 GN=Pigl PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 8.6e-33
Identity = 77/210 (36.67%), Postives = 126/210 (60.00%), Query Frame = 0

Query: 45  LLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPL 104
           L+V+AHPDDE+MFF+PT+  L      + +LC S GN    G IR++EL ++  +L +P 
Sbjct: 44  LVVIAHPDDEAMFFAPTMLGLARLEQQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIPP 103

Query: 105 NQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYG 164
           ++V I+D  D  D     W+ +L+A  + Q I ++G D ++TFD+ GVSGH NH  ++  
Sbjct: 104 SRVMIIDKRDFPDDPEVQWDTELVASTLLQHIHANGTDLVVTFDAEGVSGHSNHIALYKA 163

Query: 165 VQSFLL-NKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSF 224
           V++     K+P+      L S N LRKY+   D+  ++LS ++   ++  L ++   ++ 
Sbjct: 164 VRALHSGGKLPKGCSVLTLQSVNALRKYAFLLDLPWTLLSPQD---VLFVLTSKEVAQAK 223

Query: 225 LAMAQHSSQWVWFRKLFVTFSSYTYVNTLK 254
            AM+ H SQ +WFR L+V FS Y  +N+L+
Sbjct: 224 KAMSCHRSQLLWFRYLYVLFSRYMRINSLR 250

BLAST of MC06g0221 vs. ExPASy Swiss-Prot
Match: Q9HDW9 (Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpi12 PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.5e-32
Identity = 86/250 (34.40%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 12  VIWVASLCKILHISVSSSRSAFLNSDGTQK---RNVLLVVAHPDDESMFFSPTINFL-TS 71
           +IW  S   +  I+V S+ +   +S G +K    ++L V AHPDDESMFF PTI++L   
Sbjct: 1   MIWFWSTLLVTAIAVLSTANE--SSSGQEKLAVESILFVFAHPDDESMFFGPTIDYLGNQ 60

Query: 72  RGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFGKVWNHKL 131
               +H+LC+S GNAD +G++R +EL  A++  ++    V ++  P LQDG    W+   
Sbjct: 61  HSTRVHVLCLSNGNADGLGSVREKELVVAASKYQIDKTNVHVVSDPQLQDGMQAKWDPTD 120

Query: 132 LAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAWELVSTNI 191
           +AK I Q I  + I ++ITFD+ G+SGH NH   + G    +  K     + + L S NI
Sbjct: 121 VAKHISQIIERYNIKTLITFDNKGISGHPNHIACYEGAMKIV--KATPQVQVFVLESVNI 180

Query: 192 LRKYSGPFDIWLSMLSKE---NTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTFS 251
            RKY    D   +++  +   N + ++H       +     +  H SQ VWFR  ++  S
Sbjct: 181 FRKYISYLDTIPTLVQSQAGRNDTIIIHADRKSTQRIRDAMVRGHKSQMVWFRYGWIYLS 240

Query: 252 SYTYVNTLKR 255
            Y   N LKR
Sbjct: 241 KYMSNNVLKR 246

BLAST of MC06g0221 vs. ExPASy Swiss-Prot
Match: O35790 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Rattus norvegicus OX=10116 GN=Pigl PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.6e-32
Identity = 77/210 (36.67%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 45  LLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPL 104
           L+V+AHPDDE+MFF+PTI  L      + +LC S GN    G IR++EL ++  +L +P 
Sbjct: 44  LVVIAHPDDEAMFFAPTILGLARLKQQVSLLCFSSGNYYNQGEIRKKELLQSCAVLGIPP 103

Query: 105 NQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYG 164
           ++V I+D  +  D     W+ + +A  I Q I ++  D ++TFD+ GVSGH NH  ++  
Sbjct: 104 SRVMIIDKREFPDDPEVQWDTEHVASTILQHIHANATDLVVTFDAEGVSGHSNHIALYKA 163

Query: 165 VQSFLL-NKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSF 224
           V++     K+P+      L S N+LRKY    D+  ++LS +   G++  L ++   ++ 
Sbjct: 164 VRALHSGGKLPEGCSVLTLQSVNVLRKYVFLLDLPWTLLSPQ---GVLFVLTSKEVAQAK 223

Query: 225 LAMAQHSSQWVWFRKLFVTFSSYTYVNTLK 254
            AM+ H SQ +WFR L+  FS Y  VN+L+
Sbjct: 224 KAMSCHRSQLLWFRHLYTVFSRYMSVNSLQ 250

BLAST of MC06g0221 vs. ExPASy Swiss-Prot
Match: Q54C64 (Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Dictyostelium discoideum OX=44689 GN=pigl PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.6e-31
Identity = 81/242 (33.47%), Postives = 119/242 (49.17%), Query Frame = 0

Query: 41  KRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTIL 100
           K+ VL V+AHPDDE MFF+PTI      G  + + C+S GNA  +G IR +EL  +   +
Sbjct: 16  KKKVLFVIAHPDDECMFFTPTIQHYNFIGSEIFVACLSNGNAVGLGKIREKELIDSCIDM 75

Query: 101 KVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRD 160
            +    V      + QDG   +W+  L+ K I   I     D +I+FD  G+S H NH  
Sbjct: 76  GINQENVFFDQTNNFQDGMNIIWDTDLVEKTILSFIKQTSADIVISFDECGISSHPNHIS 135

Query: 161 VHYGVQSFLLNK--------------------------VPQSFEAWELVSTNILRKYSGP 220
           +  G++  + NK                          + +  +A++L + NI+RKY G 
Sbjct: 136 ISNGLKQLMKNKSSSTTTTSTTSSSSSSSSLSNRTTNNLNKEIKAYKLETVNIIRKYIGI 195

Query: 221 FDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLK 256
            DI L+ +LS +  S          P  S+  M +H SQ+VWFR LFV  S Y+++NTL 
Sbjct: 196 ADIPLTKLLSYDENSTQTFISTQLFPPSSYSPMTKHKSQFVWFRYLFVFLSRYSFINTLI 255

BLAST of MC06g0221 vs. NCBI nr
Match: XP_022135568.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X2 [Momordica charantia] >XP_022135569.1 N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X2 [Momordica charantia])

HSP 1 Score: 516 bits (1329), Expect = 1.23e-184
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP
Sbjct: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG
Sbjct: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW
Sbjct: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180

Query: 181 ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF 240
           ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF
Sbjct: 181 ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF 240

Query: 241 VTFSSYTYVNTLKRIN 256
           VTFSSYTYVNTLKRIN
Sbjct: 241 VTFSSYTYVNTLKRIN 256

BLAST of MC06g0221 vs. NCBI nr
Match: XP_023515925.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456 bits (1173), Expect = 1.42e-160
Identity = 226/257 (87.94%), Postives = 240/257 (93.39%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIW+ASLCKI H SVSSSRSAFLN    QKRN+LLV+AHPDDESMFFSP
Sbjct: 18  MAWLLVITAFVVIWIASLCKIFHASVSSSRSAFLNDGTPQKRNILLVIAHPDDESMFFSP 77

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           T+NFLTSRGH L+ILCMSIGNAD MGT+RREELYKAST+LKVPLNQVKILDHPDLQDGFG
Sbjct: 78  TMNFLTSRGHKLYILCMSIGNADGMGTMRREELYKASTVLKVPLNQVKILDHPDLQDGFG 137

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVW+HKLLA+IIEQEISSHGID IITFDSYGVSGHCNHRDVH GV+ FL NK PQSFEAW
Sbjct: 138 KVWDHKLLAEIIEQEISSHGIDLIITFDSYGVSGHCNHRDVHNGVRGFLHNKAPQSFEAW 197

Query: 181 ELVSTNILRKYSGPFDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           ELVSTNILRKYSGPFDIWLS M SKE+ SGMMHCLLNE P++SFLAMAQH+SQWVWFRKL
Sbjct: 198 ELVSTNILRKYSGPFDIWLSSMFSKEHPSGMMHCLLNERPRQSFLAMAQHASQWVWFRKL 257

Query: 241 FVTFSSYTYVNTLKRIN 256
           FVTFSSYTYVNTLKRIN
Sbjct: 258 FVTFSSYTYVNTLKRIN 274

BLAST of MC06g0221 vs. NCBI nr
Match: XP_022135567.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Momordica charantia])

HSP 1 Score: 454 bits (1169), Expect = 6.21e-160
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 37  DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA 96
           DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA
Sbjct: 57  DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA 116

Query: 97  STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC 156
           STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC
Sbjct: 117 STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC 176

Query: 157 NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN 216
           NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN
Sbjct: 177 NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN 236

Query: 217 ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN 256
           ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN
Sbjct: 237 ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN 276

BLAST of MC06g0221 vs. NCBI nr
Match: KAG6589537.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 455 bits (1170), Expect = 3.15e-159
Identity = 227/257 (88.33%), Postives = 241/257 (93.77%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIW+ASLCK  H SVSSSRSAFLN DGTQKRN+LLV+AHPDDESMFFSP
Sbjct: 76  MAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLN-DGTQKRNILLVIAHPDDESMFFSP 135

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           T+NFLTSRGH L+ILCMSIGNAD MGT+RREELYKAST+LKVPLNQVKILDHPDLQDGFG
Sbjct: 136 TMNFLTSRGHKLYILCMSIGNADGMGTMRREELYKASTVLKVPLNQVKILDHPDLQDGFG 195

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVW+HKLLA+IIE EISSHGID IITFDSYGVSGHCNHRDVH GV+ FL NK PQSFEAW
Sbjct: 196 KVWDHKLLAEIIEHEISSHGIDLIITFDSYGVSGHCNHRDVHNGVRGFLHNKAPQSFEAW 255

Query: 181 ELVSTNILRKYSGPFDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           ELVSTNILRKYSGPFDIWLS M SKE+ SGMMHCLLNE P++SFLAMAQH+SQWVWFRKL
Sbjct: 256 ELVSTNILRKYSGPFDIWLSSMFSKEHPSGMMHCLLNERPRQSFLAMAQHASQWVWFRKL 315

Query: 241 FVTFSSYTYVNTLKRIN 256
           FVTFSSYTYVNTLKRIN
Sbjct: 316 FVTFSSYTYVNTLKRIN 331

BLAST of MC06g0221 vs. NCBI nr
Match: XP_022921645.1 (N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 451 bits (1161), Expect = 9.22e-159
Identity = 225/256 (87.89%), Postives = 240/256 (93.75%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIW+ASLCK  H SVSSSRSAFLN DGTQKRN+LLV+AHPDDESMFFSP
Sbjct: 18  MAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLN-DGTQKRNILLVIAHPDDESMFFSP 77

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           T+NFLTSRGH L+ILCMSIGNAD MGT+RREELYKAST+LKVPLNQVKILDHPDLQDGFG
Sbjct: 78  TMNFLTSRGHKLYILCMSIGNADGMGTMRREELYKASTVLKVPLNQVKILDHPDLQDGFG 137

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVW+HKLLA+IIEQEISSHGID IITFDSYGVSGHCNHRDVH GV+ FL NK PQ FEAW
Sbjct: 138 KVWDHKLLAEIIEQEISSHGIDLIITFDSYGVSGHCNHRDVHNGVRGFLHNKAPQGFEAW 197

Query: 181 ELVSTNILRKYSGPFDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           ELVSTNILRKYSGPFDIWLS M SKE++SGM HCLLNE P++SFLAMAQH+SQWVWFRKL
Sbjct: 198 ELVSTNILRKYSGPFDIWLSSMFSKEHSSGMTHCLLNERPRQSFLAMAQHASQWVWFRKL 257

Query: 241 FVTFSSYTYVNTLKRI 255
           FVTFSSYTYVNTLKRI
Sbjct: 258 FVTFSSYTYVNTLKRI 272

BLAST of MC06g0221 vs. ExPASy TrEMBL
Match: A0A6J1C1T3 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3673 GN=LOC111007488 PE=3 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 5.94e-185
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP
Sbjct: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG
Sbjct: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW
Sbjct: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180

Query: 181 ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF 240
           ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF
Sbjct: 181 ELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLF 240

Query: 241 VTFSSYTYVNTLKRIN 256
           VTFSSYTYVNTLKRIN
Sbjct: 241 VTFSSYTYVNTLKRIN 256

BLAST of MC06g0221 vs. ExPASy TrEMBL
Match: A0A6J1C326 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3673 GN=LOC111007488 PE=3 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 3.01e-160
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 37  DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA 96
           DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA
Sbjct: 57  DGTQKRNVLLVVAHPDDESMFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKA 116

Query: 97  STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC 156
           STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC
Sbjct: 117 STILKVPLNQVKILDHPDLQDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHC 176

Query: 157 NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN 216
           NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN
Sbjct: 177 NHRDVHYGVQSFLLNKVPQSFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLN 236

Query: 217 ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN 256
           ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN
Sbjct: 237 ECPQKSFLAMAQHSSQWVWFRKLFVTFSSYTYVNTLKRIN 276

BLAST of MC06g0221 vs. ExPASy TrEMBL
Match: A0A6J1E6D6 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita moschata OX=3662 GN=LOC111429838 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 4.47e-159
Identity = 225/256 (87.89%), Postives = 240/256 (93.75%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIW+ASLCK  H SVSSSRSAFLN DGTQKRN+LLV+AHPDDESMFFSP
Sbjct: 18  MAWLLVITAFVVIWIASLCKNFHASVSSSRSAFLN-DGTQKRNILLVIAHPDDESMFFSP 77

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           T+NFLTSRGH L+ILCMSIGNAD MGT+RREELYKAST+LKVPLNQVKILDHPDLQDGFG
Sbjct: 78  TMNFLTSRGHKLYILCMSIGNADGMGTMRREELYKASTVLKVPLNQVKILDHPDLQDGFG 137

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVW+HKLLA+IIEQEISSHGID IITFDSYGVSGHCNHRDVH GV+ FL NK PQ FEAW
Sbjct: 138 KVWDHKLLAEIIEQEISSHGIDLIITFDSYGVSGHCNHRDVHNGVRGFLHNKAPQGFEAW 197

Query: 181 ELVSTNILRKYSGPFDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           ELVSTNILRKYSGPFDIWLS M SKE++SGM HCLLNE P++SFLAMAQH+SQWVWFRKL
Sbjct: 198 ELVSTNILRKYSGPFDIWLSSMFSKEHSSGMTHCLLNERPRQSFLAMAQHASQWVWFRKL 257

Query: 241 FVTFSSYTYVNTLKRI 255
           FVTFSSYTYVNTLKRI
Sbjct: 258 FVTFSSYTYVNTLKRI 272

BLAST of MC06g0221 vs. ExPASy TrEMBL
Match: A0A6J1JIB1 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita maxima OX=3661 GN=LOC111485360 PE=3 SV=1)

HSP 1 Score: 450 bits (1157), Expect = 1.88e-158
Identity = 223/257 (86.77%), Postives = 238/257 (92.61%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSP 60
           MAWLLVITAFVVIW+ASLCKI H SVSSSRSAFLN    QK+N+LLV+AHPDDESMFFSP
Sbjct: 18  MAWLLVITAFVVIWIASLCKIFHASVSSSRSAFLNDGTPQKKNILLVIAHPDDESMFFSP 77

Query: 61  TINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFG 120
           T+NFLTSRGH L+ILCMSIGNAD MGT+RREELYKAST+LKVPLNQVKILDHPDLQDGFG
Sbjct: 78  TMNFLTSRGHKLYILCMSIGNADGMGTMRREELYKASTVLKVPLNQVKILDHPDLQDGFG 137

Query: 121 KVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAW 180
           KVW+HKLLA IIE EISSHGID IITFDSYGVSGHCNHRDVH GV+ FL NK PQSFEAW
Sbjct: 138 KVWDHKLLAGIIELEISSHGIDLIITFDSYGVSGHCNHRDVHNGVRGFLHNKAPQSFEAW 197

Query: 181 ELVSTNILRKYSGPFDIWLS-MLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           ELVSTNILRKYSGPFDIWLS M SKE+ SGMMHCLLNE P++S+LAMAQH+SQWVWFRKL
Sbjct: 198 ELVSTNILRKYSGPFDIWLSSMFSKEHPSGMMHCLLNERPRQSYLAMAQHASQWVWFRKL 257

Query: 241 FVTFSSYTYVNTLKRIN 256
           FVTFSSYTYVNTLKRIN
Sbjct: 258 FVTFSSYTYVNTLKRIN 274

BLAST of MC06g0221 vs. ExPASy TrEMBL
Match: A0A6J1C1E2 (N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3673 GN=LOC111007488 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 1.72e-146
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0

Query: 56  MFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDL 115
           MFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDL
Sbjct: 1   MFFSPTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDL 60

Query: 116 QDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQ 175
           QDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQ
Sbjct: 61  QDGFGKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQ 120

Query: 176 SFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVW 235
           SFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVW
Sbjct: 121 SFEAWELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVW 180

Query: 236 FRKLFVTFSSYTYVNTLKRIN 256
           FRKLFVTFSSYTYVNTLKRIN
Sbjct: 181 FRKLFVTFSSYTYVNTLKRIN 201

BLAST of MC06g0221 vs. TAIR 10
Match: AT3G58130.1 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 329.3 bits (843), Expect = 2.7e-90
Identity = 161/257 (62.65%), Postives = 198/257 (77.04%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGT-QKRNVLLVVAHPDDESMFFS 60
           MAWL+V  + +VIW+AS CKI   + S S +A L+   T QK+NVL V+AHPDDESMFFS
Sbjct: 1   MAWLVVSVSLIVIWLASFCKIFFRATSISGAAILDDGKTPQKKNVLFVIAHPDDESMFFS 60

Query: 61  PTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGF 120
           PTIN+L S   NLH+LC+S GNAD MG+IR  EL++A  +LKVPL Q+KIL+HP+LQDGF
Sbjct: 61  PTINYLASNACNLHMLCLSTGNADGMGSIRNNELHRACAVLKVPLQQLKILNHPNLQDGF 120

Query: 121 GKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEA 180
           G++W+H LL +IIE+E++ H I +IITFD+YGVSGHCNHRDVH GV  FL     ++ +A
Sbjct: 121 GQLWSHDLLTEIIEEEVTKHDIHTIITFDNYGVSGHCNHRDVHRGVLKFLQTNSGRNVKA 180

Query: 181 WELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           WELVS NI RKY GP DIWLS+LS +     +  ++NE P KSF AMAQH SQWVWFRKL
Sbjct: 181 WELVSLNIFRKYCGPVDIWLSILSAKIHPSKV-IIINEQPWKSFKAMAQHLSQWVWFRKL 240

Query: 241 FVTFSSYTYVNTLKRIN 257
           FV+FSSYTY NTL RIN
Sbjct: 241 FVSFSSYTYANTLSRIN 256

BLAST of MC06g0221 vs. TAIR 10
Match: AT3G58130.2 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 329.3 bits (843), Expect = 2.7e-90
Identity = 161/257 (62.65%), Postives = 198/257 (77.04%), Query Frame = 0

Query: 1   MAWLLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGT-QKRNVLLVVAHPDDESMFFS 60
           MAWL+V  + +VIW+AS CKI   + S S +A L+   T QK+NVL V+AHPDDESMFFS
Sbjct: 1   MAWLVVSVSLIVIWLASFCKIFFRATSISGAAILDDGKTPQKKNVLFVIAHPDDESMFFS 60

Query: 61  PTINFLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGF 120
           PTIN+L S   NLH+LC+S GNAD MG+IR  EL++A  +LKVPL Q+KIL+HP+LQDGF
Sbjct: 61  PTINYLASNACNLHMLCLSTGNADGMGSIRNNELHRACAVLKVPLQQLKILNHPNLQDGF 120

Query: 121 GKVWNHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEA 180
           G++W+H LL +IIE+E++ H I +IITFD+YGVSGHCNHRDVH GV  FL     ++ +A
Sbjct: 121 GQLWSHDLLTEIIEEEVTKHDIHTIITFDNYGVSGHCNHRDVHRGVLKFLQTNSGRNVKA 180

Query: 181 WELVSTNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKL 240
           WELVS NI RKY GP DIWLS+LS +     +  ++NE P KSF AMAQH SQWVWFRKL
Sbjct: 181 WELVSLNIFRKYCGPVDIWLSILSAKIHPSKV-IIINEQPWKSFKAMAQHLSQWVWFRKL 240

Query: 241 FVTFSSYTYVNTLKRIN 257
           FV+FSSYTY NTL RIN
Sbjct: 241 FVSFSSYTYANTLSRIN 256

BLAST of MC06g0221 vs. TAIR 10
Match: AT2G27340.2 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-85
Identity = 157/253 (62.06%), Postives = 194/253 (76.68%), Query Frame = 0

Query: 4   LLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSPTIN 63
           ++ ++  VVIWVAS  KI   + S SR+  L+ DG  K+NV+ V+AHPDDESMFFSPTIN
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILD-DG--KKNVMFVIAHPDDESMFFSPTIN 62

Query: 64  FLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFGKVW 123
           + TS   NLHILC S GNAD MG+IR +EL++A  +LKVPL Q+KILDHP+LQDGFG++W
Sbjct: 63  YFTSTACNLHILCFSTGNADGMGSIRDQELHRACAVLKVPLQQLKILDHPNLQDGFGQLW 122

Query: 124 NHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAWELV 183
           +H LL +IIE+E+++H I +IITFD+YGV GHCNHRDVH GV  FL     ++ +AWELV
Sbjct: 123 SHDLLTEIIEEEVTNHYIHTIITFDNYGVWGHCNHRDVHRGVLKFLQTNSGRNVKAWELV 182

Query: 184 STNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTF 243
           S NI RKY GP DIWLS+LS +     +  ++N+ P KSF AMAQH SQWVWFRKLFV F
Sbjct: 183 SLNIFRKYCGPVDIWLSILSAKRHPSKV-IIINKQPWKSFKAMAQHLSQWVWFRKLFVLF 242

Query: 244 SSYTYVNTLKRIN 257
           SSYTYVNTL RIN
Sbjct: 243 SSYTYVNTLDRIN 251

BLAST of MC06g0221 vs. TAIR 10
Match: AT2G27340.3 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-85
Identity = 157/253 (62.06%), Postives = 194/253 (76.68%), Query Frame = 0

Query: 4   LLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSPTIN 63
           ++ ++  VVIWVAS  KI   + S SR+  L+ DG  K+NV+ V+AHPDDESMFFSPTIN
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILD-DG--KKNVMFVIAHPDDESMFFSPTIN 62

Query: 64  FLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFGKVW 123
           + TS   NLHILC S GNAD MG+IR +EL++A  +LKVPL Q+KILDHP+LQDGFG++W
Sbjct: 63  YFTSTACNLHILCFSTGNADGMGSIRDQELHRACAVLKVPLQQLKILDHPNLQDGFGQLW 122

Query: 124 NHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAWELV 183
           +H LL +IIE+E+++H I +IITFD+YGV GHCNHRDVH GV  FL     ++ +AWELV
Sbjct: 123 SHDLLTEIIEEEVTNHYIHTIITFDNYGVWGHCNHRDVHRGVLKFLQTNSGRNVKAWELV 182

Query: 184 STNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTF 243
           S NI RKY GP DIWLS+LS +     +  ++N+ P KSF AMAQH SQWVWFRKLFV F
Sbjct: 183 SLNIFRKYCGPVDIWLSILSAKRHPSKV-IIINKQPWKSFKAMAQHLSQWVWFRKLFVLF 242

Query: 244 SSYTYVNTLKRIN 257
           SSYTYVNTL RIN
Sbjct: 243 SSYTYVNTLDRIN 251

BLAST of MC06g0221 vs. TAIR 10
Match: AT2G27340.4 (N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-85
Identity = 157/253 (62.06%), Postives = 194/253 (76.68%), Query Frame = 0

Query: 4   LLVITAFVVIWVASLCKILHISVSSSRSAFLNSDGTQKRNVLLVVAHPDDESMFFSPTIN 63
           ++ ++  VVIWVAS  KI   + S SR+  L+ DG  K+NV+ V+AHPDDESMFFSPTIN
Sbjct: 3   VVFLSLIVVIWVASFFKIFFRATSISRATILD-DG--KKNVMFVIAHPDDESMFFSPTIN 62

Query: 64  FLTSRGHNLHILCMSIGNADEMGTIRREELYKASTILKVPLNQVKILDHPDLQDGFGKVW 123
           + TS   NLHILC S GNAD MG+IR +EL++A  +LKVPL Q+KILDHP+LQDGFG++W
Sbjct: 63  YFTSTACNLHILCFSTGNADGMGSIRDQELHRACAVLKVPLQQLKILDHPNLQDGFGQLW 122

Query: 124 NHKLLAKIIEQEISSHGIDSIITFDSYGVSGHCNHRDVHYGVQSFLLNKVPQSFEAWELV 183
           +H LL +IIE+E+++H I +IITFD+YGV GHCNHRDVH GV  FL     ++ +AWELV
Sbjct: 123 SHDLLTEIIEEEVTNHYIHTIITFDNYGVWGHCNHRDVHRGVLKFLQTNSGRNVKAWELV 182

Query: 184 STNILRKYSGPFDIWLSMLSKENTSGMMHCLLNECPQKSFLAMAQHSSQWVWFRKLFVTF 243
           S NI RKY GP DIWLS+LS +     +  ++N+ P KSF AMAQH SQWVWFRKLFV F
Sbjct: 183 SLNIFRKYCGPVDIWLSILSAKRHPSKV-IIINKQPWKSFKAMAQHLSQWVWFRKLFVLF 242

Query: 244 SSYTYVNTLKRIN 257
           SSYTYVNTL RIN
Sbjct: 243 SSYTYVNTLDRIN 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y2B22.2e-3635.57N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Homo sapiens OX=9606... [more]
Q5SX198.6e-3336.67N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Mus musculus OX=1009... [more]
Q9HDW92.5e-3234.40Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Schizosacch... [more]
O357905.6e-3236.67N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Rattus norvegicus OX... [more]
Q54C641.6e-3133.47Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase OS=Dictyosteli... [more]
Match NameE-valueIdentityDescription
XP_022135568.11.23e-184100.00N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X2 [Momord... [more]
XP_023515925.11.42e-16087.94N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurb... [more]
XP_022135567.16.21e-160100.00N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Momord... [more]
KAG6589537.13.15e-15988.33N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, partial [Cucurbita arg... [more]
XP_022921645.19.22e-15987.89N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like isoform X1 [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1C1T35.94e-185100.00N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3... [more]
A0A6J1C3263.01e-160100.00N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3... [more]
A0A6J1E6D64.47e-15987.89N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita moschata OX=36... [more]
A0A6J1JIB11.88e-15886.77N-acetylglucosaminylphosphatidylinositol deacetylase OS=Cucurbita maxima OX=3661... [more]
A0A6J1C1E21.72e-146100.00N-acetylglucosaminylphosphatidylinositol deacetylase OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT3G58130.12.7e-9062.65N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT3G58130.22.7e-9062.65N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.21.2e-8562.06N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.31.2e-8562.06N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
AT2G27340.41.2e-8562.06N-acetylglucosaminylphosphatidylinositol de-N-acetylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003737N-acetylglucosaminyl phosphatidylinositol deacetylase-relatedPFAMPF02585PIG-Lcoord: 45..168
e-value: 1.8E-24
score: 86.9
IPR003737N-acetylglucosaminyl phosphatidylinositol deacetylase-relatedPANTHERPTHR12993N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATEDcoord: 1..256
IPR024078Putative deacetylase LmbE-like domain superfamilyGENE3D3.40.50.10320coord: 16..252
e-value: 1.2E-33
score: 118.5
IPR024078Putative deacetylase LmbE-like domain superfamilySUPERFAMILY102588LmbE-likecoord: 41..252
IPR039516N-acetylglucosaminyl-phosphatidylinositol de-N-acetylasePANTHERPTHR12993:SF11N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASEcoord: 1..256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0221.1MC06g0221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000225 N-acetylglucosaminylphosphatidylinositol deacetylase activity