MC06g0214 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0214
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	SNAP25 homologous protein SNAP33
Location	MC06: 1803634 .. 1806110 (-)
RNA-Seq Expression	MC06g0214
Synteny	MC06g0214

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCACATTTTAGGAAATCCCCCATAAATGTTGCTAGGCATGGCTCAGCCCAGCCTGCTTATTCTGCTCGTTCCAGTTCAAACCCAATTGATTCCAATGATGATTTAGACAAGAGGCAAAAGCATAAGTCCACAAGAAGCACCTCTGCTCACCATGCAACTGTAGCACCAAACCCCAGCTCCAATCTTTTCGAGGATCATCTTGCGAAAGAGACCGACTCTTCATCATCCACAAGTTCATATTCATGGTCGTCAAGAAACCGATATAAGAACGATTTCCGTGACTCCGGTGGGCTTGAGAATCAGACAGTAGAGGAGCTGGAGAACTATGCTACGTACAAGGCCGAGGAGACGACGACAGCAGTTAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACGAAAACTTTAGTTGCCCTGCATCAACAGGGTGAGCAAATCACTCGGACTCATGTAGTTACTGCTGATATTGACAATGATCTTAGTCGGGTACGTGGAATGAACTAACCACCATATTCATGCTGAATCAACTTCTTGAATATATGCTTAATCTTCTTATGTGCTGCTTTTTTACTTGAATTGGTGAGGATCAATGGATGTTCTTGCTTTTCTTAGCCTCCCTTTTTTTTGGGTCTCATTGGCGTTAATCATATTTTCATCCTAATGAAGTTACACATTAGAATATCTTGCCTTCCATCGACCTTACTCCAAATGAGATATTTTGTTAAGAATTTGAGTAATACAGGGTTGAAGTTACAAAAACTTAGGTCTTTTGTAACTTGCGTTTGCTTTTATGTTGGTTTCATAATTTGTAAAAAGTTTGTAGTTGTTGTTTGAGGTTTGTGGATGGAGAGAAATAATAGGATTCTTTTAGGAGTTAAAAGAGATTTTGGGAGGACGTGTGAGCTCTTGCTTGGTCTCATGTTTCCTTTAGTCTTCAGTGTCGAAAGACTTCTATAATTAGTCTCTAGGTTTGATCTTACTTCATTGGAGATCTACGCTGTTTTCTTATATGCCATTTCGTATTCTTTCATTTTTCACAATAAAAGTTCGGCTTTTCATTAAAGTATATATAGCTTTCTGAAGTTATATTATGCTAATTGTAGTTGAAACTTGTTATTCTGAAACTGTTATGTGGCACTACTGTCAAGCCTCTCATTTATGGATGATTCTTGTTAAGATCCTGAAATGATTTGGGTATTTATCATCATTATTTGATAATGCTGATTTCAGGGTGAGAAATTTCTAGGAAGTTTAGGAGGGTTATTCTCCAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTGTCCTAGCAGGTAACATCTTCAAACCTTAAAACCAAAAGAAGGAAATAAAAGTGACCATCAGCTTTGGTTATCCAAACAAATTTATACAATCCTAATCAATAAAAAAAAAAGGTACCAACATGAACATAGTTCAACAGTTAAGATATTTTGCTTCTTCCCTAACACTTGGAAGTTCAATCCTTCATCTCCAAATTTATTGTACTAAGGAAAAAAAAAGAACCAAAACGGTTTGTAAATCTTAACGTAGTAAAACTGGTTAAAGCATATATCTTCGACAAAAAATCAAAATCTCCACCCCTACACGTTGTTGAACTTATTAAAAGAAAAAACAAACAAGAAAGGTTTCAACTTTCCCACCAGATGGATGAAGCTGATGGTCAATGAGATATACTTTTAATTTAAATACTATTTTAGTCTACTTTTAGTTTTCATTTATTTTAGATCTTATACTTTCAAAATGTTTATTTTAGTCTTCCAACTCAAATTCATTTTAATCCATATATTTTCAAAATATCCGGATATTTTGATCTATGTAATGTACCTTTAAAAAGTGACAATTTCGATCCCTCTTCTTTTGTTTATAATTTATTTATTCATTTAAATGATAGAGGATCAGGCTGTAATAAGCAAGGGCAACCATTTGGAGCAGAGGGAGAAGTTGGGGTTAAGTAGGGCACCCAAGCCACGGCCCTCACGTGCCCCACACCCTGAGCCAACCAATTCACTTCAGAAAGTTGAGGTTTGTAATTTCTTAGTTTCTTTATACTCCATCACATGTAGAAAAAGTGCAAGCAAGTTTCATATGATTTCTGGAGAAGTGCTTTTGGATGGTGTCGTGGTTATATAGTTAAAAGAGTGATTTGGTAAAGCAGGTAGAAAAGGTGAAACAAGAGGATGCTCTTTCAGATTTAAGCAATATATTGGGAGAGCTTAAAAATATGGCTGTTGACATGGGATCTGAATTAGACAGGTGAGTTGTTTACTATAAATGCCAAAATTTTCTTCCATCTTCCTTAAACTTGGCTGAGACCTATCTATCTTCTCCTATTCCAGGCAGAATAAAGCTCTGGATAACCTGTTCGATGATGCTGATGAGATCACCATCCGTGTCGATAATGCTAATCGGCGTGCTCGTCACTTGCTTAGAAAG

mRNA sequence

ATGTCACATTTTAGGAAATCCCCCATAAATGTTGCTAGGCATGGCTCAGCCCAGCCTGCTTATTCTGCTCGTTCCAGTTCAAACCCAATTGATTCCAATGATGATTTAGACAAGAGGCAAAAGCATAAGTCCACAAGAAGCACCTCTGCTCACCATGCAACTGTAGCACCAAACCCCAGCTCCAATCTTTTCGAGGATCATCTTGCGAAAGAGACCGACTCTTCATCATCCACAAGTTCATATTCATGGTCGTCAAGAAACCGATATAAGAACGATTTCCGTGACTCCGGTGGGCTTGAGAATCAGACAGTAGAGGAGCTGGAGAACTATGCTACGTACAAGGCCGAGGAGACGACGACAGCAGTTAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACGAAAACTTTAGTTGCCCTGCATCAACAGGGTGAGCAAATCACTCGGACTCATGTAGTTACTGCTGATATTGACAATGATCTTAGTCGGGGTGAGAAATTTCTAGGAAGTTTAGGAGGGTTATTCTCCAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTGTCCTAGCAGAGGATCAGGCTGTAATAAGCAAGGGCAACCATTTGGAGCAGAGGGAGAAGTTGGGGTTAAGTAGGGCACCCAAGCCACGGCCCTCACGTGCCCCACACCCTGAGCCAACCAATTCACTTCAGAAAGTTGAGGTAGAAAAGGTGAAACAAGAGGATGCTCTTTCAGATTTAAGCAATATATTGGGAGAGCTTAAAAATATGGCTGTTGACATGGGATCTGAATTAGACAGGCAGAATAAAGCTCTGGATAACCTGTTCGATGATGCTGATGAGATCACCATCCGTGTCGATAATGCTAATCGGCGTGCTCGTCACTTGCTTAGAAAG

Coding sequence (CDS)

ATGTCACATTTTAGGAAATCCCCCATAAATGTTGCTAGGCATGGCTCAGCCCAGCCTGCTTATTCTGCTCGTTCCAGTTCAAACCCAATTGATTCCAATGATGATTTAGACAAGAGGCAAAAGCATAAGTCCACAAGAAGCACCTCTGCTCACCATGCAACTGTAGCACCAAACCCCAGCTCCAATCTTTTCGAGGATCATCTTGCGAAAGAGACCGACTCTTCATCATCCACAAGTTCATATTCATGGTCGTCAAGAAACCGATATAAGAACGATTTCCGTGACTCCGGTGGGCTTGAGAATCAGACAGTAGAGGAGCTGGAGAACTATGCTACGTACAAGGCCGAGGAGACGACGACAGCAGTTAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACGAAAACTTTAGTTGCCCTGCATCAACAGGGTGAGCAAATCACTCGGACTCATGTAGTTACTGCTGATATTGACAATGATCTTAGTCGGGGTGAGAAATTTCTAGGAAGTTTAGGAGGGTTATTCTCCAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTGTCCTAGCAGAGGATCAGGCTGTAATAAGCAAGGGCAACCATTTGGAGCAGAGGGAGAAGTTGGGGTTAAGTAGGGCACCCAAGCCACGGCCCTCACGTGCCCCACACCCTGAGCCAACCAATTCACTTCAGAAAGTTGAGGTAGAAAAGGTGAAACAAGAGGATGCTCTTTCAGATTTAAGCAATATATTGGGAGAGCTTAAAAATATGGCTGTTGACATGGGATCTGAATTAGACAGGCAGAATAAAGCTCTGGATAACCTGTTCGATGATGCTGATGAGATCACCATCCGTGTCGATAATGCTAATCGGCGTGCTCGTCACTTGCTTAGAAAG

Protein sequence

MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPSSNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRARHLLRK

Homology

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BLAST of MC06g0214 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.6e-80
Identity = 173/308 (56.17%), Postives = 215/308 (69.81%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLD-KRQKHKSTRSTSAHHATVAPNP 60
           M   RKSP N+ +H S        S  NP DS+D+ D K   + S R+TS        NP
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNP 60

Query: 61  SSNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETT 120
               F     ++ DSSSS  S   +S+ +YKN+FRDSGG+ENQ+V+ELE YA YKAEETT
Sbjct: 61  ----FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETT 120

Query: 121 TAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFS 180
            +V  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEK LGSLGG+FS
Sbjct: 121 KSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFS 180

Query: 181 KTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRP-SRAPHPEPTNSLQ 240
           KTWKPKK R I GPVV  +D     + NHLE+REKLGL+ AP+ +  +R P PE  ++ Q
Sbjct: 181 KTWKPKKTRPINGPVVTRDDSPT-RRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQ 240

Query: 241 KVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANR 300
           +VE+EK KQ+D LSDLS+ILGELKNMAVDMGSE+++QNK LD+L DD DE+  RV  +N+
Sbjct: 241 RVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQ 300

Query: 301 RARHLLRK 307
           R R LL K
Sbjct: 301 RGRRLLGK 300

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BLAST of MC06g0214 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 3.1e-69
Identity = 149/253 (58.89%), Postives = 181/253 (71.54%), Query Frame = 0


Query: 57  PNPSSNLFEDHL--AKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYK 116
           PN SSN     +   + T S     +  +   ++YKN F DSGGL++QT EELE YA YK
Sbjct: 14  PNESSNNKGGTITAGRRTSSEPILITPDFDDDDKYKNGFNDSGGLQSQTTEELEKYAVYK 73

Query: 117 AEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSL 176
           AEETT  VNNCLKIAED+R D  +TL  LHQQGEQI RTH +  D+D DLSRGEK L +L
Sbjct: 74  AEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNL 133

Query: 177 GGLFSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPH-PEP 236
           GG+FSK WKPKK + ITGP++  +  +  S+ NH E+REKLGL    K R S  P   +P
Sbjct: 134 GGMFSKPWKPKKTKNITGPMITPDKPSKKSE-NHKEEREKLGL--GAKGRSSSQPALDQP 193

Query: 237 TNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRV 296
           TN+LQKVE EK KQ+D LSDLS+ILG+LK+MAVDMGSE+D+QNKALD+L DD DE+  RV
Sbjct: 194 TNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRV 253

Query: 297 DNANRRARHLLRK 307
             AN+RARHLL K
Sbjct: 254 QGANQRARHLLSK 263

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BLAST of MC06g0214 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-56
Identity = 131/254 (51.57%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 58  NPSSNLFEDHLAKETDSSSSTSSYSWSS-----RNRYKNDFRDSGGLENQTVEELENYAT 117
           N S N F+D   KE   S S + +           R+ +  + SGG ENQTV+ELE+YA 
Sbjct: 5   NSSWNPFDDE--KEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAV 64

Query: 118 YKAEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLG 177
           Y +EETT  V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK LG
Sbjct: 65  YNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILG 124

Query: 178 SLGGLFSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPE 237
            LGG+FS+TWKPKK R ITGPV+   D     K   L+ REKLGL+  P  +P     PE
Sbjct: 125 RLGGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLN--PSLKPKSKTLPE 184

Query: 238 PTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIR 297
             ++ QK ++   KQ++AL+DLS +LGELKNMAVDMG+ ++RQ   LD+L D+ADE+  R
Sbjct: 185 AVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYR 244

Query: 298 VDNANRRARHLLRK 307
           V  +N+RAR+LLRK
Sbjct: 245 VKQSNQRARYLLRK 251

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BLAST of MC06g0214 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.3e-14
Identity = 63/213 (29.58%), Postives = 107/213 (50.23%), Query Frame = 0


Query: 104 VEELENYATYKAEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 163
           +EE++  A   A+E+  +    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 164 LSRGEKFLGSLG---GL---------FSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQ 223
           +   EK L  LG   GL          S  +K   G    G VV ++   V+      ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDG-VVASQPARVV------DE 130

Query: 224 REKLGLSRAPKPRPSRAPHPEPTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSE 283
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L++MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 284 LDRQNKALDNLFDDADEITIRVDNANRRARHLL 305
           +D QN+ +D + + AD    R+D AN+RA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of MC06g0214 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.3e-14
Identity = 63/213 (29.58%), Postives = 107/213 (50.23%), Query Frame = 0


Query: 104 VEELENYATYKAEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 163
           +EE++  A   A+E+  +    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 164 LSRGEKFLGSLG---GL---------FSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQ 223
           +   EK L  LG   GL          S  +K   G    G VV ++   V+      ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDG-VVASQPARVV------DE 130

Query: 224 REKLGLSRAPKPRPSRAPHPEPTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSE 283
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L++MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 284 LDRQNKALDNLFDDADEITIRVDNANRRARHLL 305
           +D QN+ +D + + AD    R+D AN+RA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of MC06g0214 vs. NCBI nr
Match: XP_022134601.1 (SNAP25 homologous protein SNAP33 [Momordica charantia])

HSP 1 Score: 583 bits (1504), Expect = 1.24e-209
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS
Sbjct: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT
Sbjct: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK
Sbjct: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 306

[Close]

BLAST of MC06g0214 vs. NCBI nr
Match: XP_022921394.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921397.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 516 bits (1329), Expect = 5.54e-183
Identity = 271/306 (88.56%), Postives = 285/306 (93.14%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPI  ARHGS+QPA  ARS+S PIDSND+LDKRQKHKS+R TS+H ATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLF+DHL KETDSSS  SSYSWSSRNRYKNDFRDSGGL++QTVEELENYA YKAEETT 
Sbjct: 61  SNLFDDHLEKETDSSSH-SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEETTN 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEK LGSLGG+FSK
Sbjct: 121 AVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPV+   DQAVISKGNHLEQRE+LGLSRAP PR SRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDADEIT+RVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 305

[Close]

BLAST of MC06g0214 vs. NCBI nr
Match: XP_023516514.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516515.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516516.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 515 bits (1326), Expect = 1.59e-182
Identity = 270/306 (88.24%), Postives = 284/306 (92.81%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPI  ARHGS+QPA  ARS S PIDSND+LDKRQKHKS+R TS+H ATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSPSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLF+DHL KETDSSS  SSYSWSSRNRYKNDFRDSGGL++QTVEELENYA YKAEETT 
Sbjct: 61  SNLFDDHLEKETDSSSH-SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEETTN 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEK LGSLGG+FSK
Sbjct: 121 AVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGP++   DQAVISKGNHLEQRE+LGLSRAP PR SRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPIIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDADEIT+RVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 305

[Close]

BLAST of MC06g0214 vs. NCBI nr
Match: XP_022987568.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022987569.1 putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022987570.1 putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 515 bits (1326), Expect = 1.59e-182
Identity = 270/306 (88.24%), Postives = 285/306 (93.14%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPI  ARHGS+QPAY ARS+S PIDSND+ DKRQKHKS+R TS+H ATVAPNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLF+DHL KETDSSS  SSYSWSSRNRYKNDFRDSGGL++QTVEELENYA YKAEETT 
Sbjct: 61  SNLFDDHLEKETDSSSH-SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEETTN 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDATKTLVALH QGEQITRTH+VTADIDNDLSRGEK LGSLGG+FSK
Sbjct: 121 AVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPV+   DQAVISKGNHLEQRE+LGLSRAP PR SRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDADEIT+RVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 305

[Close]

BLAST of MC06g0214 vs. NCBI nr
Match: XP_038879751.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879753.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 510 bits (1314), Expect = 1.24e-180
Identity = 269/309 (87.06%), Postives = 287/309 (92.88%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MS FRKSPINVARHGS+QP  SAR S NP+DSND+LDK+QK+KS+R TS+H ATVAPNPS
Sbjct: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60

Query: 61  SNLFEDHLAKETDS---SSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEE 120
           SNLF+DHL +ET+S   SSS+SSYSWSSRNRYKNDFRDSGG+ENQTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGL 180
           TT AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEK LGSLGG+
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPV+   DQAVISKGNHLEQREKLGLSRAP PR  RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELK MAVDMGSELDRQNKALD+LFDDADE+TIRVDNAN
Sbjct: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300

Query: 301 RRARHLLRK 306
           +RARHLLRK
Sbjct: 301 QRARHLLRK 309

[Close]

BLAST of MC06g0214 vs. ExPASy TrEMBL
Match: A0A6J1BY92 (SNAP25 homologous protein SNAP33 OS=Momordica charantia OX=3673 GN=LOC111006828 PE=4 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 5.99e-210
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS
Sbjct: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT
Sbjct: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK
Sbjct: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 306

[Close]

BLAST of MC06g0214 vs. ExPASy TrEMBL
Match: A0A6J1E197 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111429687 PE=4 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 2.68e-183
Identity = 271/306 (88.56%), Postives = 285/306 (93.14%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPI  ARHGS+QPA  ARS+S PIDSND+LDKRQKHKS+R TS+H ATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLF+DHL KETDSSS  SSYSWSSRNRYKNDFRDSGGL++QTVEELENYA YKAEETT 
Sbjct: 61  SNLFDDHLEKETDSSSH-SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEETTN 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEK LGSLGG+FSK
Sbjct: 121 AVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPV+   DQAVISKGNHLEQRE+LGLSRAP PR SRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELK MAVDMG+ELDRQNKALDNLFDDADEIT+RVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 305

[Close]

BLAST of MC06g0214 vs. ExPASy TrEMBL
Match: A0A6J1JJU0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111485094 PE=4 SV=1)

HSP 1 Score: 515 bits (1326), Expect = 7.68e-183
Identity = 270/306 (88.24%), Postives = 285/306 (93.14%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MSHFRKSPI  ARHGS+QPAY ARS+S PIDSND+ DKRQKHKS+R TS+H ATVAPNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETTT 120
           SNLF+DHL KETDSSS  SSYSWSSRNRYKNDFRDSGGL++QTVEELENYA YKAEETT 
Sbjct: 61  SNLFDDHLEKETDSSSH-SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEETTN 120

Query: 121 AVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFSK 180
           AVNNCLKIAEDMREDATKTLVALH QGEQITRTH+VTADIDNDLSRGEK LGSLGG+FSK
Sbjct: 121 AVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIFSK 180

Query: 181 TWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQKV 240
           TWKPKKGRQITGPV+   DQAVISKGNHLEQRE+LGLSRAP PR SRAPHPEPTNSLQKV
Sbjct: 181 TWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSLQKV 240

Query: 241 EVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANRRA 300
           EVEKVKQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDADEIT+RVDNANRRA
Sbjct: 241 EVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNANRRA 300

Query: 301 RHLLRK 306
           RHLLRK
Sbjct: 301 RHLLRK 305

[Close]

BLAST of MC06g0214 vs. ExPASy TrEMBL
Match: A0A0A0LRS5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G012100 PE=4 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.44e-174
Identity = 262/309 (84.79%), Postives = 284/309 (91.91%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MS FRKSPINV RHGS+QPA SARSS NP++SND+LDK+QK +S+R TS+H AT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFEDHLAKETDSSS---STSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEE 120
           S LF+DHL K TDSSS   S+SSYSWSSRNRYKNDFRDSGG+ENQTVEELE+YA YKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGL 180
           TT +VNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEK LGSLGG+
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPV+   D AVISKGNHLEQREKLGLSRAP PR SRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNAN 300
           QKVEVEKVKQ+DALSDLSNILGELK MAVDMGSELD+QNKALD+LFDDA+E+TIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

Query: 301 RRARHLLRK 306
           +RARHLLRK
Sbjct: 301 QRARHLLRK 308

[Close]

BLAST of MC06g0214 vs. ExPASy TrEMBL
Match: A0A5A7US66 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002820 PE=4 SV=1)

HSP 1 Score: 487 bits (1254), Expect = 7.64e-172
Identity = 260/308 (84.42%), Postives = 279/308 (90.58%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLDKRQKHKSTRSTSAHHATVAPNPS 60
           MS FRKSPINV RH S QPA  ARSS N ++SND+LDKRQK +S+R TS+H  T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFEDHLAKETDSS--SSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEET 120
           S LF+DHL K TDSS  SS+SSYSWSSRNRYKNDFRDSGG+ENQTVEELENYA YKAEET
Sbjct: 61  SALFDDHLEKGTDSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEET 120

Query: 121 TTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLF 180
           T +VNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEK LGSLGG+F
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPV+   D AVISKGNHLEQREKLGLSRAP PR SRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANR 300
           KVEVEKVKQEDALSDLSNILGELK MAVDMGSELD+QN+ALDNLFDDA+E+TIRVDNAN+
Sbjct: 241 KVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 306
           RARHLLRK
Sbjct: 301 RARHLLRK 307

[Close]

BLAST of MC06g0214 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 298.5 bits (763), Expect = 6.1e-81
Identity = 173/308 (56.17%), Postives = 215/308 (69.81%), Query Frame = 0


Query: 1   MSHFRKSPINVARHGSAQPAYSARSSSNPIDSNDDLD-KRQKHKSTRSTSAHHATVAPNP 60
           M   RKSP N+ +H S        S  NP DS+D+ D K   + S R+TS        NP
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNP 60

Query: 61  SSNLFEDHLAKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYKAEETT 120
               F     ++ DSSSS  S   +S+ +YKN+FRDSGG+ENQ+V+ELE YA YKAEETT
Sbjct: 61  ----FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETT 120

Query: 121 TAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSLGGLFS 180
            +V  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEK LGSLGG+FS
Sbjct: 121 KSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFS 180

Query: 181 KTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRP-SRAPHPEPTNSLQ 240
           KTWKPKK R I GPVV  +D     + NHLE+REKLGL+ AP+ +  +R P PE  ++ Q
Sbjct: 181 KTWKPKKTRPINGPVVTRDDSPT-RRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQ 240

Query: 241 KVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRVDNANR 300
           +VE+EK KQ+D LSDLS+ILGELKNMAVDMGSE+++QNK LD+L DD DE+  RV  +N+
Sbjct: 241 RVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQ 300

Query: 301 RARHLLRK 307
           R R LL K
Sbjct: 301 RGRRLLGK 300

[Close]

BLAST of MC06g0214 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 263.5 bits (672), Expect = 2.2e-70
Identity = 149/253 (58.89%), Postives = 181/253 (71.54%), Query Frame = 0


Query: 57  PNPSSNLFEDHL--AKETDSSSSTSSYSWSSRNRYKNDFRDSGGLENQTVEELENYATYK 116
           PN SSN     +   + T S     +  +   ++YKN F DSGGL++QT EELE YA YK
Sbjct: 14  PNESSNNKGGTITAGRRTSSEPILITPDFDDDDKYKNGFNDSGGLQSQTTEELEKYAVYK 73

Query: 117 AEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLGSL 176
           AEETT  VNNCLKIAED+R D  +TL  LHQQGEQI RTH +  D+D DLSRGEK L +L
Sbjct: 74  AEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNL 133

Query: 177 GGLFSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPH-PEP 236
           GG+FSK WKPKK + ITGP++  +  +  S+ NH E+REKLGL    K R S  P   +P
Sbjct: 134 GGMFSKPWKPKKTKNITGPMITPDKPSKKSE-NHKEEREKLGL--GAKGRSSSQPALDQP 193

Query: 237 TNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIRV 296
           TN+LQKVE EK KQ+D LSDLS+ILG+LK+MAVDMGSE+D+QNKALD+L DD DE+  RV
Sbjct: 194 TNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRV 253

Query: 297 DNANRRARHLLRK 307
             AN+RARHLL K
Sbjct: 254 QGANQRARHLLSK 263

[Close]

BLAST of MC06g0214 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 221.5 bits (563), Expect = 9.5e-58
Identity = 131/254 (51.57%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 58  NPSSNLFEDHLAKETDSSSSTSSYSWSS-----RNRYKNDFRDSGGLENQTVEELENYAT 117
           N S N F+D   KE   S S + +           R+ +  + SGG ENQTV+ELE+YA 
Sbjct: 5   NSSWNPFDDE--KEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAV 64

Query: 118 YKAEETTTAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKFLG 177
           Y +EETT  V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK LG
Sbjct: 65  YNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILG 124

Query: 178 SLGGLFSKTWKPKKGRQITGPVVLAEDQAVISKGNHLEQREKLGLSRAPKPRPSRAPHPE 237
            LGG+FS+TWKPKK R ITGPV+   D     K   L+ REKLGL+  P  +P     PE
Sbjct: 125 RLGGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLN--PSLKPKSKTLPE 184

Query: 238 PTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITIR 297
             ++ QK ++   KQ++AL+DLS +LGELKNMAVDMG+ ++RQ   LD+L D+ADE+  R
Sbjct: 185 AVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYR 244

Query: 298 VDNANRRARHLLRK 307
           V  +N+RAR+LLRK
Sbjct: 245 VKQSNQRARYLLRK 251

[Close]

BLAST of MC06g0214 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 47.0 bits (110), Expect = 3.2e-05
Identity = 28/70 (40.00%), Postives = 37/70 (52.86%), Query Frame = 0


Query: 232 EPTNSLQKVEVEKVKQEDALSDLSNILGELKNMAVDMGSELDRQNKALDNLFDDADEITI 291
           E +   Q+ E+ K+KQE  L  +S  L  LKNMA DM  ELDRQ   +D +    D  T 
Sbjct: 163 ESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATS 222

Query: 292 RVDNANRRAR 302
            + N N R +
Sbjct: 223 DLKNTNVRLK 232

The following BLAST results are available for this feature:

BLAST of MC06g0214 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7P9	8.6e-80	56.17	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9LMG8	3.1e-69	58.89	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9SD96	1.3e-56	51.57	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
Q17QQ3	6.3e-14	29.58	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]
P60878	6.3e-14	29.58	Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1	[more]

BLAST of MC06g0214 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134601.1	1.24e-209	100.00	SNAP25 homologous protein SNAP33 [Momordica charantia]	[more]
XP_022921394.1	5.54e-183	88.56	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 p...	[more]
XP_023516514.1	1.59e-182	88.24	putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022987568.1	1.59e-182	88.24	putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022987569.1 put...	[more]
XP_038879751.1	1.24e-180	87.06	SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25...	[more]

BLAST of MC06g0214 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BY92	5.99e-210	100.00	SNAP25 homologous protein SNAP33 OS=Momordica charantia OX=3673 GN=LOC111006828 ...	[more]
A0A6J1E197	2.68e-183	88.56	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JJU0	7.68e-183	88.24	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0LRS5	1.44e-174	84.79	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A5A7US66	7.64e-172	84.42	Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11...	[more]

BLAST of MC06g0214 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61210.1	6.1e-81	56.17	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT1G13890.1	2.2e-70	58.89	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G07880.1	9.5e-58	51.57	synaptosomal-associated protein SNAP25-like 29	[more]
AT3G09740.1	3.2e-05	40.00	syntaxin of plants 71	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 126..146
None	No IPR available	COILS	Coil	Coil	coord: 279..306
None	No IPR available	COILS	Coil	Coil	coord: 237..257
None	No IPR available	GENE3D	1.20.5.110		coord: 98..175 e-value: 1.3E-18 score: 68.6
None	No IPR available	GENE3D	1.20.5.110		coord: 228..306 e-value: 4.5E-21 score: 76.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..237
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..220
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 21..306
None	No IPR available	PANTHER	PTHR19305:SF30	TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATED	coord: 21..306
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 246..302 e-value: 5.25893E-13 score: 61.0352
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 102..172
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 241..304
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 107..174 e-value: 16.0 score: 1.8 coord: 236..303 e-value: 3.8E-10 score: 49.7
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 246..303 score: 15.170474
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 109..173 e-value: 7.47886E-19 score: 76.8431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0214.1	MC06g0214.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity