MC06g0178 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0178 vs. ExPASy Swiss-Prot
Match: Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)

HSP 1 Score: 614.0 bits (1582), Expect = 1.7e-174
Identity = 314/516 (60.85%), Postives = 395/516 (76.55%), Query Frame = 0

Query: 44  RRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGS 103
           R+R    + SM+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F S
Sbjct: 41  RQRFLRCTLSMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSS 100

Query: 104 GIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRL 163
           GIVRLQVPI+Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  
Sbjct: 101 GIVRLQVPIEQKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNP 160

Query: 164 VSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGS 223
           VSVAG+GSAV F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGS
Sbjct: 161 VSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGS 220

Query: 224 FYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPE 283
           FYF VPQVEFDEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   
Sbjct: 221 FYFTVPQVEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLG 280

Query: 284 VFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAW 343
           V ++  NHVPS+  +  A++ AL++I   +S L+K      L+RS +RI+T  DIDP+AW
Sbjct: 281 VIVVSKNHVPSEGAYYPAVNNALEIIKDKHSPLSKV----VLARS-SRIITDTDIDPIAW 340

Query: 344 VACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLK 403
           +A LQ EG+++YQFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++
Sbjct: 341 LARLQCEGQDAYQFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREME 400

Query: 404 IENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKS 463
           IE DLL++PKDDLEFSIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK 
Sbjct: 401 IERDLLTSPKDDLEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKR 460

Query: 464 EEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRS 523
           E+DEF IL+ LHPTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRS
Sbjct: 461 EDDEFNILTALHPTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRS 520

Query: 524 ALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQ 560
           ALVEKGLGALIYAGTGIV GS+PSSEW+ELELKISQ
Sbjct: 521 ALVEKGLGALIYAGTGIVSGSNPSSEWNELELKISQ 547

BLAST of MC06g0178 vs. ExPASy Swiss-Prot
Match: Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)

HSP 1 Score: 596.3 bits (1536), Expect = 3.6e-169
Identity = 311/526 (59.13%), Postives = 389/526 (73.95%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIV 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L K      L+R+ +RI+
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKV----VLARN-SRII 332

Query: 339 TSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRA 398
           T  DIDP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR 
Sbjct: 333 TDTDIDPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRP 392

Query: 399 RGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHL 458
           R  S+  D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHL
Sbjct: 393 RAASSARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHL 452

Query: 459 YAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGG 518
           Y+QLAGRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG 
Sbjct: 453 YSQLAGRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGE 512

Query: 519 QSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQ 560
           +SEFAVGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQ
Sbjct: 513 ESEFAVGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQ 552

BLAST of MC06g0178 vs. ExPASy Swiss-Prot
Match: Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.4e-167
Identity = 302/525 (57.52%), Postives = 392/525 (74.67%), Query Frame = 0

Query: 48  CSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVR 107
           CS S SM+GC G+P  PVGTIETR L A  T  LAME L + V  LKS  P A  SGI+R
Sbjct: 49  CSTSLSMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSA-QSGIIR 108

Query: 108 LQVPIQQQIQAIDWLRSQPQ--ILPRTFFSGRSRTLASGPLSAD------SIYNGH---- 167
           L+VPI++ I+A+DWL SQ Q  +LPR +FSGRS+   S   S D      S  NGH    
Sbjct: 109 LEVPIEEHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRI 168

Query: 168 -HPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANV 227
               +   LVSVAGVGSAV+F   + FS+ DW SIKRFLSK CPLIRAYGAIRFDAR ++
Sbjct: 169 STSSDDKNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHI 228

Query: 228 SSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNI 287
           + EW+ FGSFYF+VPQVEFDE  G+SM+AATVAWD+A S T++ A+ +LQ+T+ QVSS +
Sbjct: 229 APEWKAFGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTV 288

Query: 288 VKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVT 347
            KL++D+    ++   ++P +T WDL ++R L+ I    S LTK      L+R  ++++T
Sbjct: 289 SKLRQDVSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKV----VLARR-SQVIT 348

Query: 348 SVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRAR 407
           + DIDP+AW++  + +GK++YQFC+QP  APAFIG+TPE+LF R+ L++ SEALAATRAR
Sbjct: 349 TSDIDPLAWLSSFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRAR 408

Query: 408 GRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLY 467
           G S   DL++ +DL S+PKD+ EF+IVRE +++KL+A+C ++  EP K+VRKL RIQHLY
Sbjct: 409 GESDSLDLQMAHDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLY 468

Query: 468 AQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQ 527
           A+ AGRL+SE+DEF+ILS+LHPTPAVCG P E+AR  IAE E+FDRG+YAGPVG+FGG Q
Sbjct: 469 ARFAGRLRSEDDEFKILSSLHPTPAVCGFPMEDARKFIAENEMFDRGLYAGPVGFFGGAQ 528

Query: 528 SEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQ 560
           S+F+VGIRSAL+ K  GALIYAG G+V+GSDP+ EW ELELK SQ
Sbjct: 529 SDFSVGIRSALIGKDAGALIYAGLGVVEGSDPALEWQELELKASQ 567

BLAST of MC06g0178 vs. ExPASy Swiss-Prot
Match: P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 3.8e-33
Identity = 102/367 (27.79%), Postives = 171/367 (46.59%), Query Frame = 0

Query: 203 YGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVES 262
           +G   FD      S+W+ F    F VP +         ML  T          W +  E 
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178

Query: 263 LQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSN-SELTKAFT 322
            ++ +  + +   +               VP     D A+  A + +D+ +  +  +  T
Sbjct: 179 AEAVLEGLKAFAAEFM-------------VPDFKQEDQAVIAAAEELDKDDWLKAIETAT 238

Query: 323 TDFLSRSLTRIVTS----------VDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGST 382
           +    +   ++V +          + I+PV  +  L  + + SY F I+      F+G++
Sbjct: 239 SQIKEKQYDKVVLARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGAS 298

Query: 383 PERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEA 442
           PERL  R+G ++ S  LA +  RG +  +D +I  +LL++ K+ LE  IV   +     +
Sbjct: 299 PERLIKRDGGTVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVS 358

Query: 443 VCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHL 502
            C  +       + K   +QHL+  + G+L+     F+++  LHPTPA+ G P E+A  +
Sbjct: 359 SCSEVEKPDGPVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDV 418

Query: 503 IAETEIFDRGMYAGPVGWFGG-GQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEW 558
           I E E   RG YA P+GW       EFAV IRS L+E G  A ++AG GIV+ S+P SE+
Sbjct: 419 IREIEPMSRGWYAAPIGWIDSQDNGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEY 459

BLAST of MC06g0178 vs. ExPASy Swiss-Prot
Match: P38051 (Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE=1 SV=4)

HSP 1 Score: 142.1 bits (357), Expect = 1.9e-32
Identity = 135/471 (28.66%), Postives = 213/471 (45.22%), Query Frame = 0

Query: 93  LKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIY 152
           L  E P   G  ++ +  P++    A+ WL SQ Q  P+ ++  R+              
Sbjct: 15  LSQEIPATPGIRVIDIPFPLKDAFDALSWLASQ-QTYPQFYWQQRN-------------- 74

Query: 153 NGHHPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSK--KCPLIRAYGAIRFD- 212
                          G   AVV   + +F+  D    +RFL +  +   +R +G   FD 
Sbjct: 75  ---------------GDEEAVVLGAITRFTSLD--QAQRFLRQHPEHADLRIWGLNAFDP 134

Query: 213 ARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQ 272
           ++ N+            ++P++E+    G + L  T+            +  SLQ    Q
Sbjct: 135 SQGNL------------LLPRLEWRRCGGKATLRLTLF-----------SESSLQHDAIQ 194

Query: 273 VSSNIVKLQ--RDLPEVFI--LRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFL 332
               I  L   + LP + +   R  H P KT W   I  A + I  +  EL K      L
Sbjct: 195 AKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELATKTI--AEGELDKV----VL 254

Query: 333 SRSLTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRS 392
           +R+ T +  +  ++  A +A  +    N Y F +      AF+GS+PERL+ R   ++R+
Sbjct: 255 ARA-TDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRT 314

Query: 393 EALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVR 452
           EALA T A      +  ++   L+++ K+  E  +V E + ++L+A    + V P + +R
Sbjct: 315 EALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLR 374

Query: 453 KLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAG 512
            L ++QHL   +   L ++ D+   L  L PT AV GLP + AR  IA  E F R  YAG
Sbjct: 375 -LRKVQHLRRCIWTSL-NKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAG 420

Query: 513 PVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELK 557
             G+    QSEF V +RSA +  G    +YAG GIV+GSDP  EW E++ K
Sbjct: 435 SAGYLSLQQSEFCVSLRSAKI-SGNVVRLYAGAGIVRGSDPEQEWQEIDNK 420

BLAST of MC06g0178 vs. NCBI nr
Match: XP_022134886.1 (isochorismate synthase 2, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 552/560 (98.57%), Postives = 553/560 (98.75%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480

Query: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540
           CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI
Sbjct: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540

Query: 541 VKGSDPSSEWDELELKISQV 560
           VKGSDPSSEWDELELKISQV
Sbjct: 541 VKGSDPSSEWDELELKISQV 555

BLAST of MC06g0178 vs. NCBI nr
Match: XP_022134885.1 (isochorismate synthase 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1092 bits (2825), Expect = 0.0
Identity = 552/565 (97.70%), Postives = 553/565 (97.88%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQV-----EGKNSY 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQV     EGKNSY
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVAYMMVEGKNSY 360

Query: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420
           QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD
Sbjct: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420

Query: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH 480
           LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH
Sbjct: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH 480

Query: 481 PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY 540
           PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY
Sbjct: 481 PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY 540

Query: 541 AGTGIVKGSDPSSEWDELELKISQV 560
           AGTGIVKGSDPSSEWDELELKISQV
Sbjct: 541 AGTGIVKGSDPSSEWDELELKISQV 560

BLAST of MC06g0178 vs. NCBI nr
Match: XP_022134887.1 (isochorismate synthase 2, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 459/472 (97.25%), Postives = 460/472 (97.46%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQV-----EGKNSY 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQV     EGKNSY
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVAYMMVEGKNSY 360

Query: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420
           QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD
Sbjct: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420

Query: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467
           LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE
Sbjct: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467

BLAST of MC06g0178 vs. NCBI nr
Match: XP_038879640.1 (isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 810 bits (2093), Expect = 9.17e-291
Identity = 415/521 (79.65%), Postives = 450/521 (86.37%), Query Frame = 0

Query: 43  GRRRVCSE---SSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPP 102
           GRR V  E   S++MDGC G+    VGTIETR   A  TA LA ESLR GV KLK EAP 
Sbjct: 42  GRRWVVGEVCQSAAMDGCGGDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEAP- 101

Query: 103 AFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFN 162
           A  SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR+ A    SA +  NG HPFN
Sbjct: 102 ALTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRSRAID--SAHNTSNGSHPFN 161

Query: 163 HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWE 222
           HH L+  AGVGSAVVF RLH FSY DW SIKRFLSKKCPLIRAYG IRFD+RAN+S EWE
Sbjct: 162 HHHLLGAAGVGSAVVFHRLHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWE 221

Query: 223 PFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQR 282
           PFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENAVESLQST+ QVSSN V LQ+
Sbjct: 222 PFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQK 281

Query: 283 DLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDID 342
           DLP + I  + HVPSKT+WD A+ RALQ IDRSNSELTK      L+RS TRIVTSVDID
Sbjct: 282 DLPLIPIHSYTHVPSKTFWDRAVDRALQEIDRSNSELTKVV----LARS-TRIVTSVDID 341

Query: 343 PVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAI 402
           PV WVACLQ EGKNSYQFC+QPSNAPAFIG+TPERLFHREGL I+S+ALAATRARGRS I
Sbjct: 342 PVTWVACLQKEGKNSYQFCVQPSNAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPI 401

Query: 403 EDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAG 462
           ED+KIE+DL SNPKDDLEFSIVRET+KKKLEAVCDA++VEPHK+VRKLPRIQHLYAQL G
Sbjct: 402 EDIKIEHDLFSNPKDDLEFSIVRETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTG 461

Query: 463 RLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAV 522
           RL+SEEDEFEILSTLHPTPAVCGLPT EAR LIAETE+FDRGMYAGPVGWFGGG+SEFAV
Sbjct: 462 RLRSEEDEFEILSTLHPTPAVCGLPTNEARDLIAETEVFDRGMYAGPVGWFGGGESEFAV 521

Query: 523 GIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQV 560
           GIRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ+
Sbjct: 522 GIRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQI 554

BLAST of MC06g0178 vs. NCBI nr
Match: XP_004138153.3 (isochorismate synthase 2, chloroplastic isoform X1 [Cucumis sativus] >XP_011659970.2 isochorismate synthase 2, chloroplastic isoform X1 [Cucumis sativus] >KAE8652412.1 hypothetical protein Csa_013914 [Cucumis sativus])

HSP 1 Score: 803 bits (2074), Expect = 6.14e-288
Identity = 414/529 (78.26%), Postives = 457/529 (86.39%), Query Frame = 0

Query: 39  FRPHGRR---RVCSESSSMDGCRG---EPEVPVGTIETRNLAAAPTAKLAMESLRAGVWK 98
           +R  GRR    VC +S++MDGC G   EP   VG IETRN  A  TA LA E LR GV +
Sbjct: 32  WRERGRRWGGEVC-DSAAMDGCVGGSQEPGGTVGIIETRNFPAVQTAALATERLRRGVAE 91

Query: 99  LKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIY 158
           LKSEAP AF SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR   S   SA +I 
Sbjct: 92  LKSEAP-AFTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRPTHS---SAHNIS 151

Query: 159 NGHHPFNHHR-LVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDAR 218
           NG H FNHH  L+SVAGVGSAVVF RLH FSY DW SIKRFLSKKCPLIRAYG IRFD+R
Sbjct: 152 NGSHSFNHHHHLLSVAGVGSAVVFRRLHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSR 211

Query: 219 ANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVS 278
           AN+S EWEPFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENA+ESLQSTI QVS
Sbjct: 212 ANISPEWEPFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAIESLQSTIEQVS 271

Query: 279 SNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTR 338
           SN V LQ+DLP++ IL + HVPSKT+W+ A+ RALQ+ID SNSELTK      L+RS T+
Sbjct: 272 SNNVNLQKDLPQIPILSYTHVPSKTFWERAVDRALQVIDGSNSELTKVV----LARS-TQ 331

Query: 339 IVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAAT 398
           IVT VDIDPV WVACLQ EG NSYQFC+QPSNAPAFIG+TPERLFHREGL I+S+ALAAT
Sbjct: 332 IVTGVDIDPVTWVACLQKEGNNSYQFCVQPSNAPAFIGNTPERLFHREGLGIKSDALAAT 391

Query: 399 RARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQ 458
           RARGR+AIED+KIE+DL SNPK+DLEFSIVRET+KKKLEAVCDA++VEP+K+VRKLPRIQ
Sbjct: 392 RARGRTAIEDIKIEHDLFSNPKEDLEFSIVRETIKKKLEAVCDAVVVEPYKSVRKLPRIQ 451

Query: 459 HLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFG 518
           HLYAQL GRL+SEEDEFEILSTLHPTPAVCGLPTEEAR  IAETE+FDRGMYAGPVGWFG
Sbjct: 452 HLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDFIAETEVFDRGMYAGPVGWFG 511

Query: 519 GGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQV 560
           GG+SEFAVGIRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ+
Sbjct: 512 GGESEFAVGIRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQI 550

BLAST of MC06g0178 vs. ExPASy TrEMBL
Match: A0A6J1BZ14 (Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1)

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 552/560 (98.57%), Postives = 553/560 (98.75%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVEGKNSYQFCIQ 360

Query: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420
           PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI
Sbjct: 361 PSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSI 420

Query: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480
           VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV
Sbjct: 421 VRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAV 480

Query: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540
           CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI
Sbjct: 481 CGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGI 540

Query: 541 VKGSDPSSEWDELELKISQV 560
           VKGSDPSSEWDELELKISQV
Sbjct: 541 VKGSDPSSEWDELELKISQV 555

BLAST of MC06g0178 vs. ExPASy TrEMBL
Match: A0A6J1BZK7 (Isochorismate synthase OS=Momordica charantia OX=3673 GN=LOC111007036 PE=3 SV=1)

HSP 1 Score: 1092 bits (2825), Expect = 0.0
Identity = 552/565 (97.70%), Postives = 553/565 (97.88%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQV-----EGKNSY 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQV     EGKNSY
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVAYMMVEGKNSY 360

Query: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420
           QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD
Sbjct: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420

Query: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH 480
           LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH
Sbjct: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLH 480

Query: 481 PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY 540
           PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY
Sbjct: 481 PTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIY 540

Query: 541 AGTGIVKGSDPSSEWDELELKISQV 560
           AGTGIVKGSDPSSEWDELELKISQV
Sbjct: 541 AGTGIVKGSDPSSEWDELELKISQV 560

BLAST of MC06g0178 vs. ExPASy TrEMBL
Match: A0A6J1C0W3 (isochorismate synthase 2, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007036 PE=4 SV=1)

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 459/472 (97.25%), Postives = 460/472 (97.46%), Query Frame = 0

Query: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60
           MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE
Sbjct: 1   MAAVGGGGLSLGHCSKDGAESVRIASPHQVSTTGRCLRFRPHGRRRVCSESSSMDGCRGE 60

Query: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120
           PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID
Sbjct: 61  PEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAID 120

Query: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180
           WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ
Sbjct: 121 WLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQ 180

Query: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240
           FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS
Sbjct: 181 FSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNS 240

Query: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300
           MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL
Sbjct: 241 MLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDL 300

Query: 301 AIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQV-----EGKNSY 360
           AIHRALQMIDRSNSELTK      L+RS TRIVTSVDIDPVAWVACLQV     EGKNSY
Sbjct: 301 AIHRALQMIDRSNSELTKVV----LARS-TRIVTSVDIDPVAWVACLQVAYMMVEGKNSY 360

Query: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420
           QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD
Sbjct: 361 QFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDD 420

Query: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467
           LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE
Sbjct: 421 LEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDE 467

BLAST of MC06g0178 vs. ExPASy TrEMBL
Match: A0A0A0LS91 (Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 2.97e-288
Identity = 417/539 (77.37%), Postives = 459/539 (85.16%), Query Frame = 0

Query: 35  RCLRFRP------HGRR---RVCSESSSMDGCRG---EPEVPVGTIETRNLAAAPTAKLA 94
           RC R  P       GRR    VC +S++MDGC G   EP   VG IETRN  A  TA LA
Sbjct: 22  RCRRISPAVAWRERGRRWGGEVC-DSAAMDGCVGGSQEPGGTVGIIETRNFPAVQTAALA 81

Query: 95  MESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLA 154
            E LR GV +LKSEAP AF SGI+RLQVPIQQ+IQAIDWL SQP +LPRTFFSGRSR   
Sbjct: 82  TERLRRGVAELKSEAP-AFTSGIIRLQVPIQQRIQAIDWLSSQPHLLPRTFFSGRSRPTH 141

Query: 155 SGPLSADSIYNGHHPFNHHR-LVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIR 214
           S   SA +I NG H FNHH  L+SVAGVGSAVVF RLH FSY DW SIKRFLSKKCPLIR
Sbjct: 142 S---SAHNISNGSHSFNHHHHLLSVAGVGSAVVFRRLHCFSYNDWASIKRFLSKKCPLIR 201

Query: 215 AYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVE 274
           AYG IRFD+RAN+S EWEPFGSFYFMVPQVEFDEFE NSMLAATVAWDHACSWTWENA+E
Sbjct: 202 AYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEENSMLAATVAWDHACSWTWENAIE 261

Query: 275 SLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFT 334
           SLQSTI QVSSN V LQ+DLP++ IL + HVPSKT+W+ A+ RALQ+ID SNSELTK   
Sbjct: 262 SLQSTIEQVSSNNVNLQKDLPQIPILSYTHVPSKTFWERAVDRALQVIDGSNSELTKVV- 321

Query: 335 TDFLSRSLTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGL 394
              L+RS T+IVT VDIDPV WVACLQ EG NSYQFC+QPSNAPAFIG+TPERLFHREGL
Sbjct: 322 ---LARS-TQIVTGVDIDPVTWVACLQKEGNNSYQFCVQPSNAPAFIGNTPERLFHREGL 381

Query: 395 SIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPH 454
            I+S+ALAATRARGR+AIED+KIE+DL SNPK+DLEFSIVRET+KKKLEAVCDA++VEP+
Sbjct: 382 GIKSDALAATRARGRTAIEDIKIEHDLFSNPKEDLEFSIVRETIKKKLEAVCDAVVVEPY 441

Query: 455 KAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRG 514
           K+VRKLPRIQHLYAQL GRL+SEEDEFEILSTLHPTPAVCGLPTEEAR  IAETE+FDRG
Sbjct: 442 KSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDFIAETEVFDRG 501

Query: 515 MYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQV 560
           MYAGPVGWFGGG+SEFAVGIRSALV++GLGALIYAGTGIVKGS+PSSEWDELELKISQ+
Sbjct: 502 MYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVKGSNPSSEWDELELKISQI 550

BLAST of MC06g0178 vs. ExPASy TrEMBL
Match: A0A6J1EW89 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 5.52e-280
Identity = 406/550 (73.82%), Postives = 452/550 (82.18%), Query Frame = 0

Query: 30  VSTTGRCLRFRPH---GRR----------------RVCSESSSMDGCRGEPEVPVGTIET 89
            S TG C+ F  H   GRR                 V  +S +MDGCR +    V T+ET
Sbjct: 11  TSPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGCRADHGGAVETMET 70

Query: 90  RNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAIDWLRSQPQILP 149
           R   A PT  LA E+LR  V KLKSE P AF SGI+RLQ+PIQ+ I+AIDWL SQP +LP
Sbjct: 71  RTFPAVPTVGLATENLRGLVAKLKSEKP-AFSSGIIRLQLPIQRHIKAIDWLCSQPLLLP 130

Query: 150 RTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFCRLHQFSYTDWTSIK 209
           R FFSGRSR + S PL A S  N HH     RL SVAGVGSAVVFCR HQFS +DW SIK
Sbjct: 131 RIFFSGRSRAIDSDPLPAVSS-NAHH-----RLASVAGVGSAVVFCRHHQFSNSDWASIK 190

Query: 210 RFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDH 269
           R LSKKCPLIRAYG IRFD+RAN+S EWEPFGSFYFMVPQVEFDEFE +S+LAATVAWDH
Sbjct: 191 RLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDH 250

Query: 270 ACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMID 329
           ACSWTWENAVESLQSTI QVSS+ V +QRDLP++ IL HNHVP+KT+WD AI+RALQ+ID
Sbjct: 251 ACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFWDHAINRALQVID 310

Query: 330 RSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGS 389
           + +S+LTK      L+RS TRIVTSVD+DP+ WVACLQ EGKNSYQFC+QPSNAPAFIG+
Sbjct: 311 QCDSDLTKVV----LARS-TRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSNAPAFIGN 370

Query: 390 TPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLE 449
           TPERLFHREGLSI  EALAATRARGRSAIEDLKIE DLLSNPKDDLEFSIVRE +K+KLE
Sbjct: 371 TPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKLE 430

Query: 450 AVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARH 509
           AVCDA++VEPHK+VRKLPRIQHLYAQLAGRL++++DEFEILSTLHPTPAVCG PTEEAR 
Sbjct: 431 AVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARD 490

Query: 510 LIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEW 560
           LIAETE+FDRGMYAGPVGWFGG +SEFAVGIRSALVE GLGAL+YAGTGIVKGS+PS EW
Sbjct: 491 LIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKGSNPSLEW 548

BLAST of MC06g0178 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 614.0 bits (1582), Expect = 1.2e-175
Identity = 314/516 (60.85%), Postives = 395/516 (76.55%), Query Frame = 0

Query: 44  RRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGS 103
           R+R    + SM+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F S
Sbjct: 41  RQRFLRCTLSMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSS 100

Query: 104 GIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRL 163
           GIVRLQVPI+Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  
Sbjct: 101 GIVRLQVPIEQKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNP 160

Query: 164 VSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGS 223
           VSVAG+GSAV F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGS
Sbjct: 161 VSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGS 220

Query: 224 FYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPE 283
           FYF VPQVEFDEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   
Sbjct: 221 FYFTVPQVEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLG 280

Query: 284 VFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAW 343
           V ++  NHVPS+  +  A++ AL++I   +S L+K      L+RS +RI+T  DIDP+AW
Sbjct: 281 VIVVSKNHVPSEGAYYPAVNNALEIIKDKHSPLSKV----VLARS-SRIITDTDIDPIAW 340

Query: 344 VACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLK 403
           +A LQ EG+++YQFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++
Sbjct: 341 LARLQCEGQDAYQFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREME 400

Query: 404 IENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKS 463
           IE DLL++PKDDLEFSIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK 
Sbjct: 401 IERDLLTSPKDDLEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKR 460

Query: 464 EEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRS 523
           E+DEF IL+ LHPTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRS
Sbjct: 461 EDDEFNILTALHPTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRS 520

Query: 524 ALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQ 560
           ALVEKGLGALIYAGTGIV GS+PSSEW+ELELKISQ
Sbjct: 521 ALVEKGLGALIYAGTGIVSGSNPSSEWNELELKISQ 547

BLAST of MC06g0178 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 611.3 bits (1575), Expect = 7.8e-175
Identity = 311/506 (61.46%), Postives = 390/506 (77.08%), Query Frame = 0

Query: 54  MDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQ 113
           M+GC  + + P+GT+ETR L+  P+   A E L   V  LKS+ PP F SGIVRLQVPI+
Sbjct: 1   MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQ-PPPFSSGIVRLQVPIE 60

Query: 114 QQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAV 173
           Q+I AIDWL +Q +ILPR+FFS RS +     L   S  NG    + H  VSVAG+GSAV
Sbjct: 61  QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS---SDHNPVSVAGIGSAV 120

Query: 174 VFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARANVSSEWEPFGSFYFMVPQVEF 233
            F  L  FS+ DW SI+RFLS K PLIRAYG +RFD    ++ EWE FGSFYF VPQVEF
Sbjct: 121 FFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEF 180

Query: 234 DEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSNIVKLQRDLPEVFILRHNHVP 293
           DEF G+SMLAATVAWD+  SWT ENA+E+LQ T+ QVSS I++L+R+   V ++  NHVP
Sbjct: 181 DEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVP 240

Query: 294 SKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIVTSVDIDPVAWVACLQVEGKN 353
           S+  +  A++ AL++I   +S L+K      L+RS +RI+T  DIDP+AW+A LQ EG++
Sbjct: 241 SEGAYYPAVNNALEIIKDKHSPLSKV----VLARS-SRIITDTDIDPIAWLARLQCEGQD 300

Query: 354 SYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRARGRSAIEDLKIENDLLSNPK 413
           +YQFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DLL++PK
Sbjct: 301 AYQFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPK 360

Query: 414 DDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHLYAQLAGRLKSEEDEFEILST 473
           DDLEFSIVRE +++KL+ +CD ++V+PHK+VRKL R+QHLY+QLAG+LK E+DEF IL+ 
Sbjct: 361 DDLEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTA 420

Query: 474 LHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGGQSEFAVGIRSALVEKGLGAL 533
           LHPTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGG+SEF+VGIRSALVEKGLGAL
Sbjct: 421 LHPTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGAL 480

Query: 534 IYAGTGIVKGSDPSSEWDELELKISQ 560
           IYAGTGIV GS+PSSEW+ELELKISQ
Sbjct: 481 IYAGTGIVSGSNPSSEWNELELKISQ 497

BLAST of MC06g0178 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 598.2 bits (1541), Expect = 6.8e-171
Identity = 312/527 (59.20%), Postives = 390/527 (74.00%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIV 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L K      L+R+ +RI+
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKV----VLARN-SRII 332

Query: 339 TSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRA 398
           T  DIDP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR 
Sbjct: 333 TDTDIDPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRP 392

Query: 399 RGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHL 458
           R  S+  D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHL
Sbjct: 393 RAASSARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHL 452

Query: 459 YAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGG 518
           Y+QLAGRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG 
Sbjct: 453 YSQLAGRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGE 512

Query: 519 QSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQV 561
           +SEFAVGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQV
Sbjct: 513 ESEFAVGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQV 553

BLAST of MC06g0178 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.6e-170
Identity = 311/526 (59.13%), Postives = 389/526 (73.95%), Query Frame = 0

Query: 39  FRPHGRRRVCSESSSMDGCRGEPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAP 98
           F    R++  S S SM+GC G+ + P+GT+ETR + A  +   A E L + V +LKS+ P
Sbjct: 33  FTRFSRKKYESCSMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQ-P 92

Query: 99  PAFGSGIVRLQVPIQQQIQAIDWLRSQPQILPRTFFSGRSRTLASGPLSADSIYNGHHPF 158
           P+F SG+VRLQVPI QQI AIDWL++Q +I PR FFS RS       L   +  NG+   
Sbjct: 93  PSFSSGVVRLQVPIDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNG 152

Query: 159 N-----HHRLVSVAGVGSAVVFCRLHQFSYTDWTSIKRFLSKKCPLIRAYGAIRFDARAN 218
           N        LVSVAG+GSAV F  L  FS+ DW SI+RFLS   PLIRAYG +RFD    
Sbjct: 153 NGTVSSDRNLVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGK 212

Query: 219 VSSEWEPFGSFYFMVPQVEFDEFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 278
           ++ EWEPFG+FYF VPQVEF+EF G+SMLAAT+AWD   SWT ENA+E+LQ T+ QVSS 
Sbjct: 213 IAVEWEPFGAFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV 272

Query: 279 IVKLQRDLPEVFILRHNHVPSKTYWDLAIHRALQMIDRSNSELTKAFTTDFLSRSLTRIV 338
           ++KL+     V +L  NHVP+K  +  A+ +AL+MI++ +S L K      L+R+ +RI+
Sbjct: 273 VMKLRNRSLGVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKV----VLARN-SRII 332

Query: 339 TSVDIDPVAWVACLQVEGKNSYQFCIQPSNAPAFIGSTPERLFHREGLSIRSEALAATRA 398
           T  DIDP+AW+A LQ EG ++YQFC+QP  APAFIG+TPERLF R  L + SEALAATR 
Sbjct: 333 TDTDIDPIAWLAQLQREGHDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRP 392

Query: 399 RGRSAIEDLKIENDLLSNPKDDLEFSIVRETMKKKLEAVCDAIIVEPHKAVRKLPRIQHL 458
           R  S+  D++IE DLL++PKDDLEFSIVRE +++KL  +CD ++V+P K VRKL R+QHL
Sbjct: 393 RAASSARDMEIERDLLTSPKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHL 452

Query: 459 YAQLAGRLKSEEDEFEILSTLHPTPAVCGLPTEEARHLIAETEIFDRGMYAGPVGWFGGG 518
           Y+QLAGRL  E+DE++IL+ LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG 
Sbjct: 453 YSQLAGRLTKEDDEYKILAALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGE 512

Query: 519 QSEFAVGIRSALVEKGLGALIYAGTGIVKGSDPSSEWDELELKISQ 560
           +SEFAVGIRSALVEKGLGALIYAGTGIV GSDPSSEW+EL+LKISQ
Sbjct: 513 ESEFAVGIRSALVEKGLGALIYAGTGIVAGSDPSSEWNELDLKISQ 552

BLAST of MC06g0178 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 104.0 bits (258), Expect = 4.0e-22
Identity = 73/219 (33.33%), Postives = 112/219 (51.14%), Query Frame = 0

Query: 60  EPEVPVGTIETRNLAAAPTAKLAMESLRAGVWKLKSEAPPAFGSGIVRLQVPIQQQIQAI 119
           + ++ V    TR L  A T +L +ESL+  + +LK+  PP   SG++R QV +  + +A+
Sbjct: 71  DDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTN-PPKSSSGVLRFQVAVPPRAKAL 130

Query: 120 DWLRSQP---QILPRTFFSGRSRTLASGPLSADSIYNGHHPFNHHRLVSVAGVGSAVVFC 179
            W  SQP    + P  FF           LS D++   +          V G+G+A  F 
Sbjct: 131 FWFCSQPTTSDVFP-VFF-----------LSKDTVEPSYKSLYVKEPHGVFGIGNAFAFV 190

Query: 180 RLHQFSYTDWTSIKRFLSKKCPLIRAYG--AIRFDARANVSSEWEPFGSFYFMVPQVEFD 239
                     + IK FLS +  ++ AYG   I F+  + V+S+    GS YF VPQ+E D
Sbjct: 191 HSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYFFVPQIELD 250

Query: 240 EFEGNSMLAATVAWDHACSWTWENAVESLQSTIRQVSSN 274
           E E  S+LA T+AW+ + S+T E  + S + +I QVSS+
Sbjct: 251 EHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSH 273