MC06g0176 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0176 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 7.0e-206
Identity = 376/579 (64.94%), Postives = 449/579 (77.55%), Query Frame = 0

Query: 28  EDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAAN 87
           E Y DWR R AV+GRHGGMLAASFVLVVE+LENLAYLANASNLVLYL ++MH SPS +AN
Sbjct: 11  EGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSAN 70

Query: 88  IVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCFP 147
            VTNFMGTAF LALLGGFL+DAFF+T+ IFLISA+IE LGL+ILT+QA  P+L PP+C  
Sbjct: 71  DVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-- 130

Query: 148 NQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFFN 207
                 C  V G +A MLF GLYL ALGVGGIKGSL  HGAEQFD +T +GRK+RS FFN
Sbjct: 131 --DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN 190

Query: 208 YFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGSP 267
           YF+FCL+CGAL+AVT VVW+EDNKGW+WGFG+ST+ I +SI IFL GS  YR K P GSP
Sbjct: 191 YFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSP 250

Query: 268 ITTIFKVLASAAFNCRKSKRLNKVVMSMSTS----SVSSDRDVDDHHHIQSQSREKNPSD 327
           +TTI KVL +A+  C  S   +  V SMS S     VS  +   +      + R++    
Sbjct: 251 LTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALP 310

Query: 328 DQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLS 387
            +      L+ LN AA   P H ++L+CTV+QVE+ K V++  PIF  TIMLNCCLAQLS
Sbjct: 311 PRAQLTNSLKVLNGAADEKPVH-RLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLS 370

Query: 388 TFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQL 447
           TFSVQQAA+MNTK+GSLK+PPASLP+FPV+FIMILAP Y+H+I+P ARK T+TE G+T L
Sbjct: 371 TFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 430

Query: 448 QRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLF 507
           QRIG GL LS++AMAVAALVE KRK +A   G+LDS E LP+TFLW+ALQYLFLGSADLF
Sbjct: 431 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 490

Query: 508 SLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSS 567
           +LAG++E+FFTEAP SMRSLAT+LSWASLAMGYY SSV+V+++N +T + G  PWL G S
Sbjct: 491 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 550

Query: 568 LNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTG 603
           +N Y L+ FYW+MCVLS  NFLHYLFWA RY   YRSTG
Sbjct: 551 INRYKLDYFYWLMCVLSAANFLHYLFWAMRYK--YRSTG 582

BLAST of MC06g0176 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 697.2 bits (1798), Expect = 1.7e-199
Identity = 380/595 (63.87%), Postives = 448/595 (75.29%), Query Frame = 0

Query: 16  EETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLS 75
           E+ DE         YVDWR RPA+RGRHGGMLAASFVLVVEVLENLA+LANASNLVLYLS
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 76  KFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQA 135
             M +SPSGAAN VT FMGTAFFLALLGGFLADAFFTT+ I+L+SAAIE LGL++LT+QA
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 136 HVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTT 195
           H  + +P                    V LF GLYL ALGVGGIKGSLPPHGAEQFD  T
Sbjct: 150 HEHSTEP-----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEET 209

Query: 196 LEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGS 255
             GR++RS FFNYFIF LSCGALIAVT+VVW+EDNKGW +GFG+ST  I+IS+P+FL GS
Sbjct: 210 SSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGS 269

Query: 256 PTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQ 315
             YR+K P+GSPITT+FKVL +A +   K +R +++V++  T +     D DD     S 
Sbjct: 270 RVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRN-----DCDD-----SV 329

Query: 316 SREKNPSDDQDSTDQGL--EFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTI 375
           +++    DD      G    FL +        P+ L+CT +QV++ K V++  PIF STI
Sbjct: 330 TKQNCDGDD------GFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTI 389

Query: 376 MLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKV 435
           MLNCCLAQLSTFSVQQA+TMNTKLGS  VPPA+LPVFPV+F+MILAP YNH+++P+ARK 
Sbjct: 390 MLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKS 449

Query: 436 TRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAV-----KRGMLDSAEPLPMTFL 495
           T+TE GIT LQRIGTGL LS+VAMAVAALVETKRK++ V           S+ PLP+TFL
Sbjct: 450 TKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFL 509

Query: 496 WVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVING 555
           WVA+QY+FLGSADLF+LAGMMEFFFTEAP +MRSLAT+LSWASLAMGYYFSSVLV+ +N 
Sbjct: 510 WVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNF 569

Query: 556 VTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGG 604
           VT      PWL G +LN YHLERFYW+MCVLSG+NFLHYLFWA R  Y YRS  G
Sbjct: 570 VTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASR--YVYRSNQG 589

BLAST of MC06g0176 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 2.5e-195
Identity = 367/575 (63.83%), Postives = 443/575 (77.04%), Query Frame = 0

Query: 28  EDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAAN 87
           E Y DWR + A+RGRHGGMLAASFVL VE+LENLA+LANASNLVLYL  FMH S + +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 88  IVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCFP 147
            VT FM TAF LALLGGFLADAFF+T+ IFLISA+IE LGL++LT+QA  P+L PP C  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC-K 132

Query: 148 NQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFFN 207
           +     C  VGG +A  LF GLYL +LG+GGIKGSLP HGAEQFD  T +GRK+RS FFN
Sbjct: 133 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 208 YFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGSP 267
           Y++FCLSCGAL+AVT VVWIEDNKGW+WGFG+ST++I +SI +FLLGS  Y+ K P GSP
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 268 ITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSDDQDS 327
           +TTIFKVL +A+     SK         ++S+  + R+V      QS+  EK PS    S
Sbjct: 253 LTTIFKVLLAASIVSCSSK---------TSSNHFTSREV------QSEHEEKTPS---QS 312

Query: 328 TDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLSTFSV 387
               L  LNK AI    H   L+CTV+QVE+ K V++  PIFG TIMLNCCLAQLST+SV
Sbjct: 313 LTNSLTCLNK-AIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 372

Query: 388 QQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQLQRIG 447
            QAATMN K+ +  VP ASLPVFPV+F++ILAP Y+H+I+P ARKVT++E+GIT LQRIG
Sbjct: 373 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 432

Query: 448 TGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLFSLAG 507
            GL LS+VAMAVAALVE KRK +A + G+LDS E LP+TFLW+ALQYLFLGSADLF+LAG
Sbjct: 433 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 492

Query: 508 MMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSSLNHY 567
           ++EFFFTEAP SMRSLAT+LSWASLA+GYY SSV+V ++N VTK+ G +PWL G  LN  
Sbjct: 493 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 552

Query: 568 HLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTG 603
            L+ FYW+MCVLS +NFLHYLFWAKRY Y   STG
Sbjct: 553 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYI--STG 564

BLAST of MC06g0176 vs. ExPASy Swiss-Prot
Match: Q9LSF0 (Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 4.0e-108
Identity = 241/569 (42.36%), Postives = 340/569 (59.75%), Query Frame = 0

Query: 27  DEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAA 86
           +E + DWR + A+ G+HGG+ AA    VVE +EN+ +LA ++N ++Y +K M+YS   AA
Sbjct: 6   EEKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYSTPKAA 65

Query: 87  NIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCF 146
            +VTNF+GT+F L + GGF+AD+F T ++ F++  +IE LGL++LTLQAH+  L+P    
Sbjct: 66  TMVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLGLIMLTLQAHITKLQP---- 125

Query: 147 PNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFF 206
               GG  ++    ++ +LF GLY  A+GVGG+KGSLP HG +Q  GT    ++  S FF
Sbjct: 126 ---QGG--KKPSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGGDQI-GT--RNQRLISGFF 185

Query: 207 NYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGS 266
           N++ F +  G  +AVTL+VWIE+N GW   F IST  +  +I +F+ G P YR K PAGS
Sbjct: 186 NWYFFSVCLGGFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKRPAGS 245

Query: 267 PITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSDDQD 326
           P+T I  V  SAA N       N+ V         +D +V   +H          S D+ 
Sbjct: 246 PLTRIVNVFVSAARN------RNRFV---------TDAEVVTQNH---------NSTDKS 305

Query: 327 STDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLSTFS 386
                 +FLNKA +NN       K +  QVEE +  +   PIFGSTI++NCC+AQ+ TFS
Sbjct: 306 IHHNKFKFLNKAKLNN-------KISATQVEETRTFLALLPIFGSTIIMNCCVAQMGTFS 365

Query: 387 VQQAATMNTKLG-SLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQLQR 446
           VQQ    N KL  S ++P ASL   P+L ++     Y      I     R+      L+R
Sbjct: 366 VQQGMVTNRKLSRSFEIPVASLNAIPLLCMLSSLALYELFGKRILSNSERSSS--FNLKR 425

Query: 447 IGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLFSL 506
           IG GL L+ ++MAVAA+VE KRK+ AV          + ++  W+ LQ++ L  +D+ ++
Sbjct: 426 IGYGLALTSISMAVAAIVEVKRKHEAVHNN-------IKISVFWLELQFVMLSLSDMLTV 485

Query: 507 AGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSSLN 566
            GM+EFFF E+P SMRS++TAL W S AMG++ SSVLV V+NG+T       WL    LN
Sbjct: 486 GGMLEFFFRESPASMRSMSTALGWCSTAMGFFLSSVLVEVVNGIT------GWL-RDDLN 515

Query: 567 HYHLERFYWVMCVLSGLNFLHYLFWAKRY 595
              LE FY V+CVL+ LN  +Y+FWAKRY
Sbjct: 546 ESRLELFYLVLCVLNTLNLFNYIFWAKRY 515

BLAST of MC06g0176 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.8e-105
Identity = 234/572 (40.91%), Postives = 331/572 (57.87%), Query Frame = 0

Query: 24  NVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPS 83
           N  +E + DW+ + A+ G+HGG+ AAS V VV ++EN+ ++AN  N V Y    MHY+P+
Sbjct: 3   NDMEEKFEDWKGKEAIPGKHGGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPA 62

Query: 84  GAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPP 143
            AAN+VTNFMGT+F L L GGF+AD+F T ++ F++   IE +GL++LT QAH P L   
Sbjct: 63  TAANMVTNFMGTSFLLTLFGGFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKL--- 122

Query: 144 TCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRS 203
              P +   P       ++ +LF GLY  A+G GG+K SLP HG +Q D       +  S
Sbjct: 123 --LPEKDKTPSTL----QSAILFTGLYAMAIGTGGLKASLPSHGGDQID---RRNPRLIS 182

Query: 204 AFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTP 263
            FF++  F +  G L+AVT+V+WIE+ KGW W F IS   +  ++ IF +G P YR K P
Sbjct: 183 RFFDWLYFSICSGCLLAVTVVLWIEEKKGWIWSFNISVGILATALCIFTVGLPFYRFKRP 242

Query: 264 AGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSD 323
            GSP+  I  V+ SAA N  KS                   D+D+       S  KN S 
Sbjct: 243 NGSPLKKIAIVIISAARNRNKS-------------------DLDEEMMRGLISIYKNNSH 302

Query: 324 DQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLS 383
           ++      L++++KA +N          +  +VEE +  +   PIFGSTI+++CC+AQLS
Sbjct: 303 NK------LKWIDKATLNK-------NISETEVEETRTFLGLLPIFGSTIVMSCCVAQLS 362

Query: 384 TFSVQQAATMNTKL-GSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQ 443
           TFS QQ   MN KL  S ++P  SL   P++F+++  P Y      I+            
Sbjct: 363 TFSAQQGMLMNKKLFHSFEIPVPSLTAIPLIFMLLSIPLYEFFGKKIS-SGNNNRSSSFN 422

Query: 444 LQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADL 503
           L+RIG GL LS V+MAV+A+VE KRK+  V            ++ LW+  QYL L  +D+
Sbjct: 423 LKRIGLGLALSSVSMAVSAIVEAKRKHEVVHNN-------FRISVLWLVFQYLMLSVSDM 482

Query: 504 FSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGS 563
            +L GM+EFF+ EAP +M+S++TAL W S A+G++ S+ LV V N VT   G   WL G 
Sbjct: 483 LTLGGMLEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVEVTNAVTGRLG-HQWLGGE 521

Query: 564 SLNHYHLERFYWVMCVLSGLNFLHYLFWAKRY 595
            LN   LE FY ++CVL+ LN L+Y+FWAKRY
Sbjct: 543 DLNKTRLELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of MC06g0176 vs. NCBI nr
Match: XP_022135001.1 (protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia])

HSP 1 Score: 1181 bits (3056), Expect = 0.0
Identity = 607/612 (99.18%), Postives = 608/612 (99.35%), Query Frame = 0

Query: 8   PLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA 67
           PL  +  VEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA
Sbjct: 22  PLAGQGAVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA 81

Query: 68  SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG 127
           SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG
Sbjct: 82  SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG 141

Query: 128 LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG 187
           LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG
Sbjct: 142 LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG 201

Query: 188 AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS 247
           AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS
Sbjct: 202 AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS 261

Query: 248 IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD 307
           IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD
Sbjct: 262 IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD 321

Query: 308 DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP 367
           DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP
Sbjct: 322 DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP 381

Query: 368 IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV 427
           IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV
Sbjct: 382 IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV 441

Query: 428 PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF 487
           PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF
Sbjct: 442 PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF 501

Query: 488 LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN 547
           LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN
Sbjct: 502 LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN 561

Query: 548 GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF 607
           GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF
Sbjct: 562 GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF 621

Query: 608 KKQRNSLDILVV 619
           KKQRNSLDILVV
Sbjct: 622 KKQRNSLDILVV 633

BLAST of MC06g0176 vs. NCBI nr
Match: XP_023531985.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972 bits (2513), Expect = 0.0
Identity = 506/620 (81.61%), Postives = 553/620 (89.19%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSS+TVTP EA+KHVEET E  L++ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSLTVTPSEAQKHVEETPE--LHIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           AAIESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 AAIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTIV SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK+K  N VV+S+STSSV
Sbjct: 241 TLTIVASIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNPDHP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPDHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF ST+MLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTVMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVVFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +S G +P FK+Q++  D++V
Sbjct: 601 KSAGEQPPFKEQQHKSDLVV 605

BLAST of MC06g0176 vs. NCBI nr
Match: XP_022933938.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucurbita moschata] >KAG7021500.1 Protein NRT1/ PTR FAMILY 4.6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 506/620 (81.61%), Postives = 553/620 (89.19%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KHVEET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKHVEETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           AAIESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 AAIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTI++SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK+K  N VV+S+STSSV
Sbjct: 241 TLTILVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +  G +P FK+Q++  D++V
Sbjct: 601 KIAGEQPPFKEQQHKSDLVV 605

BLAST of MC06g0176 vs. NCBI nr
Match: KAG6587520.1 (Protein NRT1/ PTR FAMILY 4.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 505/620 (81.45%), Postives = 551/620 (88.87%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KHVEET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKHVEETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           A IESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 ATIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTI++SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK K  N VV+S+STSSV
Sbjct: 241 TLTILVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKPKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +  G +P FK+Q++  D++V
Sbjct: 601 KIAGEQPPFKEQQHKSDLVV 605

BLAST of MC06g0176 vs. NCBI nr
Match: XP_022933940.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 505/620 (81.45%), Postives = 552/620 (89.03%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KH EET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKH-EETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           AAIESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 AAIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTI++SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK+K  N VV+S+STSSV
Sbjct: 241 TLTILVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +  G +P FK+Q++  D++V
Sbjct: 601 KIAGEQPPFKEQQHKSDLVV 604

BLAST of MC06g0176 vs. ExPASy TrEMBL
Match: A0A6J1BZD2 (protein NRT1/ PTR FAMILY 4.6-like OS=Momordica charantia OX=3673 GN=LOC111007112 PE=3 SV=1)

HSP 1 Score: 1181 bits (3056), Expect = 0.0
Identity = 607/612 (99.18%), Postives = 608/612 (99.35%), Query Frame = 0

Query: 8   PLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA 67
           PL  +  VEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA
Sbjct: 22  PLAGQGAVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANA 81

Query: 68  SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG 127
           SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG
Sbjct: 82  SNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLG 141

Query: 128 LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG 187
           LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG
Sbjct: 142 LLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHG 201

Query: 188 AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS 247
           AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS
Sbjct: 202 AEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVIS 261

Query: 248 IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD 307
           IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD
Sbjct: 262 IPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVD 321

Query: 308 DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP 367
           DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP
Sbjct: 322 DHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFP 381

Query: 368 IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV 427
           IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV
Sbjct: 382 IFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIV 441

Query: 428 PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF 487
           PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF
Sbjct: 442 PIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTF 501

Query: 488 LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN 547
           LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN
Sbjct: 502 LWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVIN 561

Query: 548 GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF 607
           GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF
Sbjct: 562 GVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGGEPSF 621

Query: 608 KKQRNSLDILVV 619
           KKQRNSLDILVV
Sbjct: 622 KKQRNSLDILVV 633

BLAST of MC06g0176 vs. ExPASy TrEMBL
Match: A0A6J1F691 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441202 PE=3 SV=1)

HSP 1 Score: 972 bits (2512), Expect = 0.0
Identity = 506/620 (81.61%), Postives = 553/620 (89.19%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KHVEET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKHVEETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           AAIESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 AAIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTI++SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK+K  N VV+S+STSSV
Sbjct: 241 TLTILVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +  G +P FK+Q++  D++V
Sbjct: 601 KIAGEQPPFKEQQHKSDLVV 605

BLAST of MC06g0176 vs. ExPASy TrEMBL
Match: A0A6J1F688 (protein NRT1/ PTR FAMILY 4.6-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441202 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 505/620 (81.45%), Postives = 552/620 (89.03%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KH EET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKH-EETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           AAIESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLFAGLYL ALGVGGIK
Sbjct: 121 AAIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFAGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT EGR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFEGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTI++SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN RK+K  N VV+S+STSSV
Sbjct: 241 TLTILVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+NNP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMNNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVETKRKN+A+K  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVETKRKNVALKTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +  G +P FK+Q++  D++V
Sbjct: 601 KIAGEQPPFKEQQHKSDLVV 604

BLAST of MC06g0176 vs. ExPASy TrEMBL
Match: A0A6J1I4N8 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470954 PE=3 SV=1)

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 500/620 (80.65%), Postives = 548/620 (88.39%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KHVEET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKHVEETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           A IESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLF+GLYL ALGVGGIK
Sbjct: 121 AVIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFSGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT  GR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFVGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTIV+SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAF  RK+K  N VV+S+STSSV
Sbjct: 241 TLTIVVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFISRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+ NP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMKNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVE KRKN+A++  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVEMKRKNVALRTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +S G +P FK+Q++   ++V
Sbjct: 601 KSAGEQPPFKEQQHKSHLVV 605

BLAST of MC06g0176 vs. ExPASy TrEMBL
Match: A0A6J1I8H5 (protein NRT1/ PTR FAMILY 4.6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470954 PE=3 SV=1)

HSP 1 Score: 952 bits (2462), Expect = 0.0
Identity = 499/620 (80.48%), Postives = 547/620 (88.23%), Query Frame = 0

Query: 1   MSSITVTPLEAEKHVEETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLEN 60
           MSSITVTP EA+KH EET E  LN+ +EDYVDWRKRPAV+GRHGGMLAASFVL VEVLEN
Sbjct: 1   MSSITVTPSEAQKH-EETPE--LNIWEEDYVDWRKRPAVKGRHGGMLAASFVLAVEVLEN 60

Query: 61  LAYLANASNLVLYLSKFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLIS 120
           LA+LANASNLVLYLSKFMHYSPSG+ANIVTNFMGTAF LALLGGFLADAFFTTYSIFL+S
Sbjct: 61  LAFLANASNLVLYLSKFMHYSPSGSANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLLS 120

Query: 121 AAIESLGLLILTLQAHVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIK 180
           A IESLGLLILTLQAHVP LKPPTC  NQ G PC +VGGG+AVMLF+GLYL ALGVGGIK
Sbjct: 121 AVIESLGLLILTLQAHVPYLKPPTCVSNQLGSPCHKVGGGKAVMLFSGLYLVALGVGGIK 180

Query: 181 GSLPPHGAEQFDGTTLEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGIS 240
           GSLPPHGAEQFD TT  GR KRSAFFNYFIFCLSCGALIAVTLVVW+EDNKGW+WGFGIS
Sbjct: 181 GSLPPHGAEQFDETTFVGRNKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGIS 240

Query: 241 TLTIVISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSV 300
           TLTIV+SIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAF  RK+K  N VV+S+STSSV
Sbjct: 241 TLTIVVSIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFISRKAKSSNNVVISISTSSV 300

Query: 301 SSD--RDVDDHHHIQSQSREKNPSDDQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEE 360
           SSD  RDVD+   I                 Q LEFLN+AA+ NP+HP+ LKCT+KQVEE
Sbjct: 301 SSDSVRDVDEQTPITP------------IPTQNLEFLNRAAMKNPNHPE-LKCTLKQVEE 360

Query: 361 AKAVVRTFPIFGSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMIL 420
           AK V++ FPIF STIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPV+FIMIL
Sbjct: 361 AKVVLKIFPIFASTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVIFIMIL 420

Query: 421 APAYNHVIVPIARKVTRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLD 480
           AP YNH IVPIARK+T++EMGIT LQRIGTGL LS+VAMAVAALVE KRKN+A++  MLD
Sbjct: 421 APTYNHAIVPIARKLTKSEMGITHLQRIGTGLVLSIVAMAVAALVEMKRKNVALRTHMLD 480

Query: 481 SAEPLPMTFLWVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYF 540
           S+EPLP++FLWVALQYLFLGSADLFSLAGMMEFFFTEAP+SMRSLATALSWASLAMGYYF
Sbjct: 481 SSEPLPISFLWVALQYLFLGSADLFSLAGMMEFFFTEAPMSMRSLATALSWASLAMGYYF 540

Query: 541 SSVLVTVINGVTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTY 600
           SSVLV+VINGVTKAC + PWLYG SLNHYHLERFYW+MC+LSGLNFL YLFWA RY+Y  
Sbjct: 541 SSVLVSVINGVTKACRLTPWLYGRSLNHYHLERFYWLMCILSGLNFLQYLFWANRYTYRS 600

Query: 601 RSTGGEPSFKKQRNSLDILV 618
           +S G +P FK+Q++   ++V
Sbjct: 601 KSAGEQPPFKEQQHKSHLVV 604

BLAST of MC06g0176 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 718.4 bits (1853), Expect = 5.0e-207
Identity = 376/579 (64.94%), Postives = 449/579 (77.55%), Query Frame = 0

Query: 28  EDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAAN 87
           E Y DWR R AV+GRHGGMLAASFVLVVE+LENLAYLANASNLVLYL ++MH SPS +AN
Sbjct: 11  EGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSAN 70

Query: 88  IVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCFP 147
            VTNFMGTAF LALLGGFL+DAFF+T+ IFLISA+IE LGL+ILT+QA  P+L PP+C  
Sbjct: 71  DVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-- 130

Query: 148 NQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFFN 207
                 C  V G +A MLF GLYL ALGVGGIKGSL  HGAEQFD +T +GRK+RS FFN
Sbjct: 131 --DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN 190

Query: 208 YFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGSP 267
           YF+FCL+CGAL+AVT VVW+EDNKGW+WGFG+ST+ I +SI IFL GS  YR K P GSP
Sbjct: 191 YFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSP 250

Query: 268 ITTIFKVLASAAFNCRKSKRLNKVVMSMSTS----SVSSDRDVDDHHHIQSQSREKNPSD 327
           +TTI KVL +A+  C  S   +  V SMS S     VS  +   +      + R++    
Sbjct: 251 LTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALP 310

Query: 328 DQDSTDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLS 387
            +      L+ LN AA   P H ++L+CTV+QVE+ K V++  PIF  TIMLNCCLAQLS
Sbjct: 311 PRAQLTNSLKVLNGAADEKPVH-RLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLS 370

Query: 388 TFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQL 447
           TFSVQQAA+MNTK+GSLK+PPASLP+FPV+FIMILAP Y+H+I+P ARK T+TE G+T L
Sbjct: 371 TFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 430

Query: 448 QRIGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLF 507
           QRIG GL LS++AMAVAALVE KRK +A   G+LDS E LP+TFLW+ALQYLFLGSADLF
Sbjct: 431 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 490

Query: 508 SLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSS 567
           +LAG++E+FFTEAP SMRSLAT+LSWASLAMGYY SSV+V+++N +T + G  PWL G S
Sbjct: 491 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 550

Query: 568 LNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTG 603
           +N Y L+ FYW+MCVLS  NFLHYLFWA RY   YRSTG
Sbjct: 551 INRYKLDYFYWLMCVLSAANFLHYLFWAMRYK--YRSTG 582

BLAST of MC06g0176 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 697.2 bits (1798), Expect = 1.2e-200
Identity = 380/595 (63.87%), Postives = 448/595 (75.29%), Query Frame = 0

Query: 16  EETDEHELNVCDEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLS 75
           E+ DE         YVDWR RPA+RGRHGGMLAASFVLVVEVLENLA+LANASNLVLYLS
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 76  KFMHYSPSGAANIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQA 135
             M +SPSGAAN VT FMGTAFFLALLGGFLADAFFTT+ I+L+SAAIE LGL++LT+QA
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 136 HVPNLKPPTCFPNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTT 195
           H  + +P                    V LF GLYL ALGVGGIKGSLPPHGAEQFD  T
Sbjct: 150 HEHSTEP-----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEET 209

Query: 196 LEGRKKRSAFFNYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGS 255
             GR++RS FFNYFIF LSCGALIAVT+VVW+EDNKGW +GFG+ST  I+IS+P+FL GS
Sbjct: 210 SSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGS 269

Query: 256 PTYRIKTPAGSPITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQ 315
             YR+K P+GSPITT+FKVL +A +   K +R +++V++  T +     D DD     S 
Sbjct: 270 RVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRN-----DCDD-----SV 329

Query: 316 SREKNPSDDQDSTDQGL--EFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTI 375
           +++    DD      G    FL +        P+ L+CT +QV++ K V++  PIF STI
Sbjct: 330 TKQNCDGDD------GFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTI 389

Query: 376 MLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKV 435
           MLNCCLAQLSTFSVQQA+TMNTKLGS  VPPA+LPVFPV+F+MILAP YNH+++P+ARK 
Sbjct: 390 MLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKS 449

Query: 436 TRTEMGITQLQRIGTGLFLSVVAMAVAALVETKRKNLAV-----KRGMLDSAEPLPMTFL 495
           T+TE GIT LQRIGTGL LS+VAMAVAALVETKRK++ V           S+ PLP+TFL
Sbjct: 450 TKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFL 509

Query: 496 WVALQYLFLGSADLFSLAGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVING 555
           WVA+QY+FLGSADLF+LAGMMEFFFTEAP +MRSLAT+LSWASLAMGYYFSSVLV+ +N 
Sbjct: 510 WVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNF 569

Query: 556 VTKACGVAPWLYGSSLNHYHLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTGG 604
           VT      PWL G +LN YHLERFYW+MCVLSG+NFLHYLFWA R  Y YRS  G
Sbjct: 570 VTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASR--YVYRSNQG 589

BLAST of MC06g0176 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.8e-196
Identity = 367/575 (63.83%), Postives = 443/575 (77.04%), Query Frame = 0

Query: 28  EDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAAN 87
           E Y DWR + A+RGRHGGMLAASFVL VE+LENLA+LANASNLVLYL  FMH S + +++
Sbjct: 9   EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 68

Query: 88  IVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCFP 147
            VT FM TAF LALLGGFLADAFF+T+ IFLISA+IE LGL++LT+QA  P+L PP C  
Sbjct: 69  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC-K 128

Query: 148 NQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFFN 207
           +     C  VGG +A  LF GLYL +LG+GGIKGSLP HGAEQFD  T +GRK+RS FFN
Sbjct: 129 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 188

Query: 208 YFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGSP 267
           Y++FCLSCGAL+AVT VVWIEDNKGW+WGFG+ST++I +SI +FLLGS  Y+ K P GSP
Sbjct: 189 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 248

Query: 268 ITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSDDQDS 327
           +TTIFKVL +A+     SK         ++S+  + R+V      QS+  EK PS    S
Sbjct: 249 LTTIFKVLLAASIVSCSSK---------TSSNHFTSREV------QSEHEEKTPS---QS 308

Query: 328 TDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLSTFSV 387
               L  LNK AI    H   L+CTV+QVE+ K V++  PIFG TIMLNCCLAQLST+SV
Sbjct: 309 LTNSLTCLNK-AIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 368

Query: 388 QQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQLQRIG 447
            QAATMN K+ +  VP ASLPVFPV+F++ILAP Y+H+I+P ARKVT++E+GIT LQRIG
Sbjct: 369 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 428

Query: 448 TGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLFSLAG 507
            GL LS+VAMAVAALVE KRK +A + G+LDS E LP+TFLW+ALQYLFLGSADLF+LAG
Sbjct: 429 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 488

Query: 508 MMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSSLNHY 567
           ++EFFFTEAP SMRSLAT+LSWASLA+GYY SSV+V ++N VTK+ G +PWL G  LN  
Sbjct: 489 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 548

Query: 568 HLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTG 603
            L+ FYW+MCVLS +NFLHYLFWAKRY Y   STG
Sbjct: 549 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYI--STG 560

BLAST of MC06g0176 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.8e-196
Identity = 367/575 (63.83%), Postives = 443/575 (77.04%), Query Frame = 0

Query: 28  EDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAAN 87
           E Y DWR + A+RGRHGGMLAASFVL VE+LENLA+LANASNLVLYL  FMH S + +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 88  IVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCFP 147
            VT FM TAF LALLGGFLADAFF+T+ IFLISA+IE LGL++LT+QA  P+L PP C  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPC-K 132

Query: 148 NQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFFN 207
           +     C  VGG +A  LF GLYL +LG+GGIKGSLP HGAEQFD  T +GRK+RS FFN
Sbjct: 133 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 208 YFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGSP 267
           Y++FCLSCGAL+AVT VVWIEDNKGW+WGFG+ST++I +SI +FLLGS  Y+ K P GSP
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 268 ITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSDDQDS 327
           +TTIFKVL +A+     SK         ++S+  + R+V      QS+  EK PS    S
Sbjct: 253 LTTIFKVLLAASIVSCSSK---------TSSNHFTSREV------QSEHEEKTPS---QS 312

Query: 328 TDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLSTFSV 387
               L  LNK AI    H   L+CTV+QVE+ K V++  PIFG TIMLNCCLAQLST+SV
Sbjct: 313 LTNSLTCLNK-AIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSV 372

Query: 388 QQAATMNTKLGSLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQLQRIG 447
            QAATMN K+ +  VP ASLPVFPV+F++ILAP Y+H+I+P ARKVT++E+GIT LQRIG
Sbjct: 373 HQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIG 432

Query: 448 TGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLFSLAG 507
            GL LS+VAMAVAALVE KRK +A + G+LDS E LP+TFLW+ALQYLFLGSADLF+LAG
Sbjct: 433 VGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAG 492

Query: 508 MMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSSLNHY 567
           ++EFFFTEAP SMRSLAT+LSWASLA+GYY SSV+V ++N VTK+ G +PWL G  LN  
Sbjct: 493 LLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRN 552

Query: 568 HLERFYWVMCVLSGLNFLHYLFWAKRYSYTYRSTG 603
            L+ FYW+MCVLS +NFLHYLFWAKRY Y   STG
Sbjct: 553 RLDLFYWLMCVLSVVNFLHYLFWAKRYKYI--STG 564

BLAST of MC06g0176 vs. TAIR 10
Match: AT3G25260.1 (Major facilitator superfamily protein )

HSP 1 Score: 393.7 bits (1010), Expect = 2.8e-109
Identity = 241/569 (42.36%), Postives = 340/569 (59.75%), Query Frame = 0

Query: 27  DEDYVDWRKRPAVRGRHGGMLAASFVLVVEVLENLAYLANASNLVLYLSKFMHYSPSGAA 86
           +E + DWR + A+ G+HGG+ AA    VVE +EN+ +LA ++N ++Y +K M+YS   AA
Sbjct: 6   EEKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYSTPKAA 65

Query: 87  NIVTNFMGTAFFLALLGGFLADAFFTTYSIFLISAAIESLGLLILTLQAHVPNLKPPTCF 146
            +VTNF+GT+F L + GGF+AD+F T ++ F++  +IE LGL++LTLQAH+  L+P    
Sbjct: 66  TMVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLGLIMLTLQAHITKLQP---- 125

Query: 147 PNQPGGPCRRVGGGEAVMLFAGLYLAALGVGGIKGSLPPHGAEQFDGTTLEGRKKRSAFF 206
               GG  ++    ++ +LF GLY  A+GVGG+KGSLP HG +Q  GT    ++  S FF
Sbjct: 126 ---QGG--KKPSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGGDQI-GT--RNQRLISGFF 185

Query: 207 NYFIFCLSCGALIAVTLVVWIEDNKGWKWGFGISTLTIVISIPIFLLGSPTYRIKTPAGS 266
           N++ F +  G  +AVTL+VWIE+N GW   F IST  +  +I +F+ G P YR K PAGS
Sbjct: 186 NWYFFSVCLGGFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKRPAGS 245

Query: 267 PITTIFKVLASAAFNCRKSKRLNKVVMSMSTSSVSSDRDVDDHHHIQSQSREKNPSDDQD 326
           P+T I  V  SAA N       N+ V         +D +V   +H          S D+ 
Sbjct: 246 PLTRIVNVFVSAARN------RNRFV---------TDAEVVTQNH---------NSTDKS 305

Query: 327 STDQGLEFLNKAAINNPDHPQVLKCTVKQVEEAKAVVRTFPIFGSTIMLNCCLAQLSTFS 386
                 +FLNKA +NN       K +  QVEE +  +   PIFGSTI++NCC+AQ+ TFS
Sbjct: 306 IHHNKFKFLNKAKLNN-------KISATQVEETRTFLALLPIFGSTIIMNCCVAQMGTFS 365

Query: 387 VQQAATMNTKLG-SLKVPPASLPVFPVLFIMILAPAYNHVIVPIARKVTRTEMGITQLQR 446
           VQQ    N KL  S ++P ASL   P+L ++     Y      I     R+      L+R
Sbjct: 366 VQQGMVTNRKLSRSFEIPVASLNAIPLLCMLSSLALYELFGKRILSNSERSSS--FNLKR 425

Query: 447 IGTGLFLSVVAMAVAALVETKRKNLAVKRGMLDSAEPLPMTFLWVALQYLFLGSADLFSL 506
           IG GL L+ ++MAVAA+VE KRK+ AV          + ++  W+ LQ++ L  +D+ ++
Sbjct: 426 IGYGLALTSISMAVAAIVEVKRKHEAVHNN-------IKISVFWLELQFVMLSLSDMLTV 485

Query: 507 AGMMEFFFTEAPVSMRSLATALSWASLAMGYYFSSVLVTVINGVTKACGVAPWLYGSSLN 566
            GM+EFFF E+P SMRS++TAL W S AMG++ SSVLV V+NG+T       WL    LN
Sbjct: 486 GGMLEFFFRESPASMRSMSTALGWCSTAMGFFLSSVLVEVVNGIT------GWL-RDDLN 515

Query: 567 HYHLERFYWVMCVLSGLNFLHYLFWAKRY 595
              LE FY V+CVL+ LN  +Y+FWAKRY
Sbjct: 546 ESRLELFYLVLCVLNTLNLFNYIFWAKRY 515