MC06g0174 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0174 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.4e-202
Identity = 345/583 (59.18%), Postives = 440/583 (75.47%), Query Frame = 0

Query: 1   AVQKIYDGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLL 60
           +V++    + ++ YRGWK MPF+IGNETFEKLG IGTL+NLL+YLT+VFN+KSITA T++
Sbjct: 31  SVEEDVQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATII 90

Query: 61  NIFNGSTNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRC---T 120
           N F+G+ N  T + AFLCDTYFGRYKTL  A +A FLG  VI LTAA+  LHP  C    
Sbjct: 91  NAFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAA 150

Query: 121 GELCKGATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYV 180
             +C G + GQ+AFL+ G G +VVGAGGIRPCNLAFGADQFNP +E+GK+GI+SFFNWY 
Sbjct: 151 DSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYF 210

Query: 181 FTYTFAMMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTS 240
           FT+TFA ++SLT++VYVQ++VSW +GL IPA+LM +ACL+FF G K+YVK+KA+GSP+  
Sbjct: 211 FTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAG 270

Query: 241 VAQVFVVAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKE 300
           +AQV  VAIKKR LK   QPW++L+NY PP   NSKL Y+DQFRFL+KAAI+T ED+++ 
Sbjct: 271 IAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQP 330

Query: 301 DGSAAEPWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGS- 360
           DG  A+PWKLC++QQVEEVKC+VRV+PIW    +Y+   +QQ TY VFQALQS+RRLGS 
Sbjct: 331 DGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSG 390

Query: 361 -FTIPAASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTAL 420
            F IPAA+Y VF M  +++++ +YDR LVP + ++T  ++GIT+LQR G GIF A  + +
Sbjct: 391 GFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLV 450

Query: 421 VSALVEARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYK 480
           V+  VE RRR  ALT+PTLG  PRKG IS++SA WLIPQL+LAG+A+ F A+ Q+EFYYK
Sbjct: 451 VAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYK 510

Query: 481 QFPENMRSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYF 540
           QFPENMRS  GS+F+      SYL   LI  VHR +  S  G+WL EDLNKGRLD FY+ 
Sbjct: 511 QFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFM 570

Query: 541 ITGIEFVNLGYFLVCANWYKYKGAPQNPSEIHL---ISKQQEK 575
           I GI  VN  YFLV + WY+YKG+    +       I KQQ+K
Sbjct: 571 IAGILAVNFAYFLVMSRWYRYKGSDDEVTTYETNENIIKQQDK 613

BLAST of MC06g0174 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 689.9 bits (1779), Expect = 2.5e-197
Identity = 337/577 (58.41%), Postives = 436/577 (75.56%), Query Frame = 0

Query: 2   VQKIYDGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLN 61
           V    + + ++ YRGWK MPF+IGNETFEKLG IGTL+NLL+YLTSVFN+KS TA T++N
Sbjct: 50  VDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIIN 109

Query: 62  IFNGSTNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGEL- 121
            F+G+ N  T I AFLCDTYFGRYKTL  A +A FLG  VI LTAA+ +LHP  C  ++ 
Sbjct: 110 AFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKIS 169

Query: 122 CKGATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTY 181
           C+G + GQ+ FL+ G G +VVGAGGIRPCNLAFGADQFNP +E+GKKGINSFFNWY FT+
Sbjct: 170 CEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTF 229

Query: 182 TFAMMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQ 241
           TFA ++SLT +VY+Q++VSW +GL IP  LM +AC++FF G ++YVKVKA+GSP+  +A+
Sbjct: 230 TFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIAR 289

Query: 242 VFVVAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGS 301
           V   AIKKR LK   QPWV+L+N+IP    N+ L Y+DQFRFL+KAAI+T E+++  DG+
Sbjct: 290 VIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLNSDGT 349

Query: 302 AAEPWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGS--FT 361
           A++PWKLC++QQVEEVKC+VRVIPIW    +Y+ A + Q TY VFQALQS+RRLGS  F 
Sbjct: 350 ASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFR 409

Query: 362 IPAASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSA 421
           IPAA+Y VF M  +++++  YDR LVP L ++T  E+GI++LQR G G   A ++ LVS 
Sbjct: 410 IPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSG 469

Query: 422 LVEARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFP 481
            +E RRR  ALT+PTLG  PR G IS++SA WLIPQLTLAG+A+ F A+ Q+EFYYKQFP
Sbjct: 470 FIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFP 529

Query: 482 ENMRSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITG 541
           ENM+S  GS+F+      SYL+  LI+ VHR +  S SG+WL EDLNK +LDYFY+ +TG
Sbjct: 530 ENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTG 589

Query: 542 IEFVNLGYFLVCANWYKYKGAPQNPSEIHLISKQQEK 575
           +  VN+ YFL+ A WY+YKG   N  +I  I   +E+
Sbjct: 590 LMVVNMAYFLLMARWYRYKGG--NDEDITEIETNEEE 624

BLAST of MC06g0174 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.7e-193
Identity = 335/577 (58.06%), Postives = 437/577 (75.74%), Query Frame = 0

Query: 7   DGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGS 66
           D E+++ YRGWK MPF+IGNETFEKLG +G+ +NL+IYLT+VFNMKSITA  ++NI+ G+
Sbjct: 13  DDESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGT 72

Query: 67  TNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCT---GELCKG 126
           +N  TI+ AFLCD+YFGRYKTL FA +A FLG + + LTA +  LHP +C    G +C G
Sbjct: 73  SNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNG 132

Query: 127 ATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFA 186
            + GQ+ FL     L+V+GAGGIRPCNL FGADQF+P T+ GK+GI SFFNWY FT+TFA
Sbjct: 133 PSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFA 192

Query: 187 MMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFV 246
            MVSLT+IVYVQ++VSW++GL IPA+LML+ C++FF GSK+YVKVKA+GSP+ S+ +V V
Sbjct: 193 QMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIV 252

Query: 247 VAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAE 306
           VAIKKR+LK P  P   L+NYI     NSKL +++QFRFL+K+AI T +D++ +DGS  +
Sbjct: 253 VAIKKRRLK-PVGP-NELYNYIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVD 312

Query: 307 PWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRL--GSFTIPA 366
            WKLCS+QQVEEVKCV+RV+P+W++  L++ A  QQ TY +FQ+LQS+RRL  GSF IPA
Sbjct: 313 AWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPA 372

Query: 367 ASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVE 426
            SYTVF ML ++I++PIYDR LVP L K T ++ GIT LQR G G+FL   + +VSA+VE
Sbjct: 373 GSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVE 432

Query: 427 ARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENM 486
             RR VALT+PTLG  PRKGAIS++S  WLIPQL L G+AD    V Q+EFYYKQFPENM
Sbjct: 433 QYRRKVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENM 492

Query: 487 RSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEF 546
           RS  GS+++C I   SYLS  L++ VH  +     G WLPEDLNKGRL+YFY+ + G+  
Sbjct: 493 RSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMT 552

Query: 547 VNLGYFLVCANWYKYKGAPQNPSEIHLISKQQEKMSV 578
           +NL YFL+ ++WY+YK       ++   S + +K+SV
Sbjct: 553 LNLAYFLLVSHWYRYKDVVAKDKDMDKTSAEFDKVSV 587

BLAST of MC06g0174 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.1e-136
Identity = 260/576 (45.14%), Postives = 371/576 (64.41%), Query Frame = 0

Query: 16  GWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGSTNLVTIIGA 75
           GW+A+ F++GNET E+LG+IG LAN ++YLT VF+++ + A  ++NI++G TNL  ++GA
Sbjct: 54  GWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGA 113

Query: 76  FLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGE---LCKGATAGQMAFL 135
           ++ DTY GR+KT+ FA+ A+ LGL+ I LTA+   LHP  C  +    C G    Q+  L
Sbjct: 114 YISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVL 173

Query: 136 MAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVSLTVIV 195
           + G   + VG+GGIRPC++ FG DQF+  TE G KG+ SFFNWY  T+T  ++++ TV+V
Sbjct: 174 LLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVV 233

Query: 196 YVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIKKRKLK 255
           Y+Q  VSW +G  IP  LM +A ++FF G K YV VK  GS  + +AQV V A KKRKLK
Sbjct: 234 YIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLK 293

Query: 256 QP--DQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKLCSV 315
            P  D   V+ ++     S+ SKL  S+QFR L+KAA++   D +  +G  A+ W+LCSV
Sbjct: 294 LPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGD-LTPEGPPADKWRLCSV 353

Query: 316 QQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLG-SFTIPAASYTVFSM 375
           Q+VEEVKC++R++PIW  G++   A + Q T+ V QAL+ +R LG  F IPA S +V S+
Sbjct: 354 QEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISL 413

Query: 376 LSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVVAL 435
           L++ I+LP YDR  VP + ++T  +SGIT+LQR G GI  A  + +V+ +VE  RR+ ++
Sbjct: 414 LTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSI 473

Query: 436 TR--PTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 495
               PT         ++ +S  WL PQL L GL + F  + Q+EF+  QFPE+MRSI  S
Sbjct: 474 NAGDPT--------GMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 533

Query: 496 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 555
           +F  + AG SYLS  L+TVVH+ S      DWL ++LN G+LDYFYY I  +  VNL YF
Sbjct: 534 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYF 593

Query: 556 LVCANWYKYK-GAP-------QNPSEIHLISKQQEK 575
             CA  Y+YK G P       ++  ++ + SK+  K
Sbjct: 594 WYCARGYRYKVGLPIEDFEEDKSSDDVEMTSKKSMK 620

BLAST of MC06g0174 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 2.2e-124
Identity = 247/558 (44.27%), Postives = 347/558 (62.19%), Query Frame = 0

Query: 16  GWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGSTNLVTIIGA 75
           GW+A+ F++GNET EKLG+IG  AN ++YL +VF+M+ + A  +  ++ G TN   ++GA
Sbjct: 17  GWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGA 76

Query: 76  FLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTG---ELCKGATAGQMAFL 135
            + D Y GR+KT+ +A++ S LGL+ + LTA L  LHPP C     + C      Q+  L
Sbjct: 77  LISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGIL 136

Query: 136 MAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVSLTVIV 195
             G G + +G+GGIRPC++ FG DQF+  TE G KG+ SFFNWY  T T  ++ S TV+V
Sbjct: 137 FLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVV 196

Query: 196 YVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIKKRKLK 255
           Y+QT VSW +G  IP  LM  A +LFFVG + YV VK  GS  + +A+V V A KKR LK
Sbjct: 197 YLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLK 256

Query: 256 QPDQPWVSLFN------YIPP--GSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEP 315
                 +SL +      Y PP    + SKL  +DQF+FL+KAA+I   D +  +G  A  
Sbjct: 257 ------ISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANK 316

Query: 316 WKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLG-SFTIPAAS 375
           W+LCS+Q+VEEVKC++RV+P+W  G++   A + Q T+ VFQA + +R +G  F IPAAS
Sbjct: 317 WRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAAS 376

Query: 376 YTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEAR 435
            TV S +++ IW+PIY+  LVP L ++  ++  +T+LQR GIGI  A L+   +  VE  
Sbjct: 377 ITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGV 436

Query: 436 RRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRS 495
           RR    TR T         ++ +S  WL   L L GL + F  +  +EF+  QFPE+MRS
Sbjct: 437 RR----TRAT--------EMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRS 496

Query: 496 IGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVN 555
           I  S+F  + A  +YLS LL+T VH++S      DWL +DL++G+LDYFYY I  +  VN
Sbjct: 497 IANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVN 552

Query: 556 LGYFLVCANWYKYKGAPQ 561
           L YF  CA+ Y+YK   Q
Sbjct: 557 LVYFWYCAHRYQYKAGSQ 552

BLAST of MC06g0174 vs. NCBI nr
Match: XP_004138148.1 (protein NRT1/ PTR FAMILY 2.11 [Cucumis sativus] >KGN63652.2 hypothetical protein Csa_013449 [Cucumis sativus])

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 486/572 (84.97%), Postives = 529/572 (92.48%), Query Frame = 0

Query: 7   DGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGS 66
           DGET++HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA T+LNIFNGS
Sbjct: 14  DGETQIHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATILNIFNGS 73

Query: 67  TNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATA 126
           TNLVT++GAFLCDTYFGRYKTLGFA +ASFLGLLVIHLTAA+K LHPP C  +LCKG TA
Sbjct: 74  TNLVTLVGAFLCDTYFGRYKTLGFAIIASFLGLLVIHLTAAVKTLHPPHCIADLCKGPTA 133

Query: 127 GQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMV 186
           GQM FL+ GFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMV
Sbjct: 134 GQMTFLLFGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMV 193

Query: 187 SLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAI 246
           S+TVIVYVQT+VSWALGLGIPA+LMLIAC+LFFVGSKIYVKVKATGSPMTSVAQV VVAI
Sbjct: 194 SITVIVYVQTNVSWALGLGIPAILMLIACILFFVGSKIYVKVKATGSPMTSVAQVLVVAI 253

Query: 247 KKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWK 306
           KKRKLKQPDQPW+SLF Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PWK
Sbjct: 254 KKRKLKQPDQPWLSLFEYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWK 313

Query: 307 LCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTV 366
           LCS+QQVEEVKC+VRV+P+W+ GVL+F  Q+QQ TYA+FQALQSNRR+G+FTIPAASYT+
Sbjct: 314 LCSMQQVEEVKCLVRVLPVWLAGVLFFATQAQQNTYAIFQALQSNRRIGNFTIPAASYTI 373

Query: 367 FSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRV 426
           F+MLSLSIWLPIYDR +VP L K T KE GITILQR GIGIFL  +  L+S LVE RRR+
Sbjct: 374 FAMLSLSIWLPIYDRIVVPFLLKFTKKEGGITILQRLGIGIFLTTMAVLLSGLVEDRRRI 433

Query: 427 VALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGG 486
           +ALT+P+LG EPRKGAISA+SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGG
Sbjct: 434 IALTKPSLGIEPRKGAISAMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGG 493

Query: 487 SMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGY 546
           SMFFCAIAGGSYL+GLLI VVHRMS  S SGDWLPEDLNKGRLDYFYYF+TGI  VNL Y
Sbjct: 494 SMFFCAIAGGSYLNGLLIIVVHRMSEGSKSGDWLPEDLNKGRLDYFYYFLTGIGLVNLCY 553

Query: 547 FLVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           FL+C+ WYKYKGAPQN SEIHLISKQ EK SV
Sbjct: 554 FLICSKWYKYKGAPQNASEIHLISKQPEKNSV 585

BLAST of MC06g0174 vs. NCBI nr
Match: XP_038879872.1 (protein NRT1/ PTR FAMILY 2.11-like [Benincasa hispida])

HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 486/572 (84.97%), Postives = 533/572 (93.18%), Query Frame = 0

Query: 7   DGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGS 66
           DGET++HY+GWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA TLL++FNGS
Sbjct: 14  DGETQIHYKGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATLLSVFNGS 73

Query: 67  TNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATA 126
           TNLVT++GAFLCDTYFGRYKTLGFA VASFLGLLVIHLTAA KNLHPP C  +LCKG + 
Sbjct: 74  TNLVTLVGAFLCDTYFGRYKTLGFAIVASFLGLLVIHLTAAFKNLHPPHCIQDLCKGPSP 133

Query: 127 GQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMV 186
           GQM FL+ GFG M++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMV
Sbjct: 134 GQMTFLLVGFGFMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMV 193

Query: 187 SLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAI 246
           SLTVIVYVQT+VSWALGLGIPA+LMLIAC+LFFVGSKIYVKV+ATGSPMTSVAQV VVAI
Sbjct: 194 SLTVIVYVQTNVSWALGLGIPAILMLIACILFFVGSKIYVKVQATGSPMTSVAQVLVVAI 253

Query: 247 KKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWK 306
           KKRKLKQP+QPW+SLF+Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PW+
Sbjct: 254 KKRKLKQPEQPWLSLFDYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWR 313

Query: 307 LCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTV 366
           LCS+QQVEEVKC+VRV+P+W+TGVL+F AQSQQQTYAVFQA+QSNRRLG+FTIPAASYTV
Sbjct: 314 LCSMQQVEEVKCLVRVLPVWLTGVLFFVAQSQQQTYAVFQAVQSNRRLGNFTIPAASYTV 373

Query: 367 FSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRV 426
           F+MLSLSIWLPIYDR +VP L K+T KE GITILQRQGIGIFL+ +T L+S +VE RRR 
Sbjct: 374 FAMLSLSIWLPIYDRIVVPFLQKVTKKEGGITILQRQGIGIFLSTMTMLLSGIVEDRRRA 433

Query: 427 VALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGG 486
           +ALT+PT+G EPRKGAIS++SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGG
Sbjct: 434 IALTKPTMGIEPRKGAISSMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGG 493

Query: 487 SMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGY 546
           SMFFCAIAGGSYL+GLLI +VHRMS  S  GDWLPEDLNKGRLDYFYYF+TGIE VNL Y
Sbjct: 494 SMFFCAIAGGSYLNGLLIILVHRMSNGSKYGDWLPEDLNKGRLDYFYYFLTGIELVNLCY 553

Query: 547 FLVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           FLVCA WYKYKGA QN SEIHLISK+ EK SV
Sbjct: 554 FLVCAKWYKYKGATQNASEIHLISKEPEKNSV 585

BLAST of MC06g0174 vs. NCBI nr
Match: XP_008453172.1 (PREDICTED: protein NRT1/ PTR FAMILY 2.11-like [Cucumis melo] >KAA0057922.1 protein NRT1/ PTR FAMILY 2.11-like [Cucumis melo var. makuwa] >TYJ98610.1 protein NRT1/ PTR FAMILY 2.11-like [Cucumis melo var. makuwa])

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 486/571 (85.11%), Postives = 529/571 (92.64%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET++HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA T+LNIFNGST
Sbjct: 15  GETQIHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATILNIFNGST 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKTLGFA VASFLGLLVIHLTAA+KNLHPP C  +LCKG TAG
Sbjct: 75  NLVTLVGAFLCDTYFGRYKTLGFAIVASFLGLLVIHLTAAVKNLHPPHCITDLCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLM GFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMVS
Sbjct: 135 QMTFLMFGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMVS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           +TVIVYVQT+VSWALGLGIPA+LMLIAC+LFFVGSKIYVKVKATGSPMTSVAQV V AIK
Sbjct: 195 ITVIVYVQTNVSWALGLGIPAILMLIACILFFVGSKIYVKVKATGSPMTSVAQVLVAAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFEYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC+VRV+P+W+ GVL+F  QSQQ TYA+FQALQSNRR+G+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLVRVLPVWLAGVLFFVTQSQQNTYAIFQALQSNRRIGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLSIWLPIYDR +VP L K T KE GITILQRQGIGIFL  +  L+S LVE RRRV+
Sbjct: 375 AMLSLSIWLPIYDRIVVPFLLKFTKKEGGITILQRQGIGIFLTTMAMLLSGLVEDRRRVI 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+P+LG EPRKGAIS++SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPSLGIEPRKGAISSMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           +FFCAIAGGSYL+GLLI VVHRMS  S SGDWLPEDLNKGRLDYFYYF+TGI  VNL YF
Sbjct: 495 IFFCAIAGGSYLNGLLIIVVHRMSEGSKSGDWLPEDLNKGRLDYFYYFLTGIGLVNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           L+C+ WYKYKGAPQN SEIHL+SKQ EK +V
Sbjct: 555 LICSKWYKYKGAPQNASEIHLMSKQPEKNTV 585

BLAST of MC06g0174 vs. NCBI nr
Match: XP_022988358.1 (protein NRT1/ PTR FAMILY 2.9-like [Cucurbita maxima])

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 477/571 (83.54%), Postives = 522/571 (91.42%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET+ HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA TLLNIFNG T
Sbjct: 15  GETQKHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATLLNIFNGCT 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKT+GF+ VASFLGLL+IH TA  KNLHPP C  ++CKG TAG
Sbjct: 75  NLVTLLGAFLCDTYFGRYKTVGFSIVASFLGLLLIHFTAVFKNLHPPHCIADVCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLMAGFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMM+S
Sbjct: 135 QMTFLMAGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMIS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           LTVIVYVQT+VSWALGLGIPA LMLIAC+LFFVGSKIYVK++ATGSPMTSVAQV VVAIK
Sbjct: 195 LTVIVYVQTNVSWALGLGIPAALMLIACILFFVGSKIYVKLEATGSPMTSVAQVLVVAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF+Y  PGSINSKLSYSDQFRFL+KAAIIT ED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFDYTTPGSINSKLSYSDQFRFLDKAAIITEEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC++RV+PIW++GVLYF AQ Q QTYAVFQALQSNRRLG+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLMRVLPIWMSGVLYFVAQLQLQTYAVFQALQSNRRLGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLS WLPIYDR +VP L K+T KE GIT LQRQGIGIFLA LT L+SA+VE RRR++
Sbjct: 375 AMLSLSFWLPIYDRIIVPFLQKVTKKEGGITTLQRQGIGIFLAILTMLLSAIVEDRRRII 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+PT+G EPRKGAIS++SASWLIPQL L GL+DGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPTVGIEPRKGAISSMSASWLIPQLLLYGLSDGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           MFFCA+A  SYL+GLLITVVHRMS  S  GDWLPEDLNKGRLDYFYYF+ GIE +NL YF
Sbjct: 495 MFFCAMAVASYLNGLLITVVHRMSRGSKYGDWLPEDLNKGRLDYFYYFVGGIELLNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           LVCA WYKYK APQN SEIH+ SKQ EK SV
Sbjct: 555 LVCAKWYKYKEAPQNASEIHMASKQPEKSSV 585

BLAST of MC06g0174 vs. NCBI nr
Match: XP_023516122.1 (protein NRT1/ PTR FAMILY 2.11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 478/571 (83.71%), Postives = 521/571 (91.24%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET+ HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA TLLNIFNG T
Sbjct: 15  GETQKHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATLLNIFNGCT 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKT+GF+ VASFLGLL+IH TAA KNLHPP C  ++CKG TAG
Sbjct: 75  NLVTLLGAFLCDTYFGRYKTVGFSIVASFLGLLLIHFTAAFKNLHPPHCIADVCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLMAGFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMM+S
Sbjct: 135 QMTFLMAGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMIS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           LTVIVYVQT+VSWALGLGIPA LMLIAC+LFFVGSKIYVK++ATGSPMTSVAQV VVAIK
Sbjct: 195 LTVIVYVQTNVSWALGLGIPAALMLIACILFFVGSKIYVKLEATGSPMTSVAQVLVVAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF+Y  PGSINSKLSYSDQFRFL+KAAIIT ED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFDYTTPGSINSKLSYSDQFRFLDKAAIITEEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC++RV+PIW+ GVLYF AQ Q QTYAVFQALQSNRRLG+ TIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLMRVLPIWMAGVLYFVAQLQLQTYAVFQALQSNRRLGNITIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLS WLPIYDR +VP L K+T KE GIT LQRQGIGIFLA LT L+SA+VE RRR++
Sbjct: 375 AMLSLSFWLPIYDRIIVPFLQKVTKKEGGITTLQRQGIGIFLAILTMLLSAIVEDRRRII 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+PT+G EPRKGAIS++SASWLIPQL L GL+DGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPTVGIEPRKGAISSMSASWLIPQLLLYGLSDGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           MFFCA+A  SYL+GLLITVVHRMS  S  GDWLPEDLNKGRLDYFYYF+ GIE +NL YF
Sbjct: 495 MFFCAMAVASYLNGLLITVVHRMSRGSKYGDWLPEDLNKGRLDYFYYFVGGIELLNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           LVCA WYKYK APQN SEIHL SKQ EK SV
Sbjct: 555 LVCAKWYKYKEAPQNASEIHLASKQPEKNSV 585

BLAST of MC06g0174 vs. ExPASy TrEMBL
Match: A0A5A7UPY3 (Protein NRT1/ PTR FAMILY 2.11-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002330 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 486/571 (85.11%), Postives = 529/571 (92.64%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET++HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA T+LNIFNGST
Sbjct: 15  GETQIHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATILNIFNGST 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKTLGFA VASFLGLLVIHLTAA+KNLHPP C  +LCKG TAG
Sbjct: 75  NLVTLVGAFLCDTYFGRYKTLGFAIVASFLGLLVIHLTAAVKNLHPPHCITDLCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLM GFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMVS
Sbjct: 135 QMTFLMFGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMVS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           +TVIVYVQT+VSWALGLGIPA+LMLIAC+LFFVGSKIYVKVKATGSPMTSVAQV V AIK
Sbjct: 195 ITVIVYVQTNVSWALGLGIPAILMLIACILFFVGSKIYVKVKATGSPMTSVAQVLVAAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFEYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC+VRV+P+W+ GVL+F  QSQQ TYA+FQALQSNRR+G+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLVRVLPVWLAGVLFFVTQSQQNTYAIFQALQSNRRIGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLSIWLPIYDR +VP L K T KE GITILQRQGIGIFL  +  L+S LVE RRRV+
Sbjct: 375 AMLSLSIWLPIYDRIVVPFLLKFTKKEGGITILQRQGIGIFLTTMAMLLSGLVEDRRRVI 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+P+LG EPRKGAIS++SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPSLGIEPRKGAISSMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           +FFCAIAGGSYL+GLLI VVHRMS  S SGDWLPEDLNKGRLDYFYYF+TGI  VNL YF
Sbjct: 495 IFFCAIAGGSYLNGLLIIVVHRMSEGSKSGDWLPEDLNKGRLDYFYYFLTGIGLVNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           L+C+ WYKYKGAPQN SEIHL+SKQ EK +V
Sbjct: 555 LICSKWYKYKGAPQNASEIHLMSKQPEKNTV 585

BLAST of MC06g0174 vs. ExPASy TrEMBL
Match: A0A1S3BWQ9 (protein NRT1/ PTR FAMILY 2.11-like OS=Cucumis melo OX=3656 GN=LOC103493967 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 486/571 (85.11%), Postives = 529/571 (92.64%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET++HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA T+LNIFNGST
Sbjct: 15  GETQIHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATILNIFNGST 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKTLGFA VASFLGLLVIHLTAA+KNLHPP C  +LCKG TAG
Sbjct: 75  NLVTLVGAFLCDTYFGRYKTLGFAIVASFLGLLVIHLTAAVKNLHPPHCITDLCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLM GFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMVS
Sbjct: 135 QMTFLMFGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMVS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           +TVIVYVQT+VSWALGLGIPA+LMLIAC+LFFVGSKIYVKVKATGSPMTSVAQV V AIK
Sbjct: 195 ITVIVYVQTNVSWALGLGIPAILMLIACILFFVGSKIYVKVKATGSPMTSVAQVLVAAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFEYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC+VRV+P+W+ GVL+F  QSQQ TYA+FQALQSNRR+G+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLVRVLPVWLAGVLFFVTQSQQNTYAIFQALQSNRRIGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLSIWLPIYDR +VP L K T KE GITILQRQGIGIFL  +  L+S LVE RRRV+
Sbjct: 375 AMLSLSIWLPIYDRIVVPFLLKFTKKEGGITILQRQGIGIFLTTMAMLLSGLVEDRRRVI 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+P+LG EPRKGAIS++SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPSLGIEPRKGAISSMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           +FFCAIAGGSYL+GLLI VVHRMS  S SGDWLPEDLNKGRLDYFYYF+TGI  VNL YF
Sbjct: 495 IFFCAIAGGSYLNGLLIIVVHRMSEGSKSGDWLPEDLNKGRLDYFYYFLTGIGLVNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           L+C+ WYKYKGAPQN SEIHL+SKQ EK +V
Sbjct: 555 LICSKWYKYKGAPQNASEIHLMSKQPEKNTV 585

BLAST of MC06g0174 vs. ExPASy TrEMBL
Match: A0A6J1JM27 (protein NRT1/ PTR FAMILY 2.9-like OS=Cucurbita maxima OX=3661 GN=LOC111485625 PE=3 SV=1)

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 477/571 (83.54%), Postives = 522/571 (91.42%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET+ HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA TLLNIFNG T
Sbjct: 15  GETQKHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATLLNIFNGCT 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKT+GF+ VASFLGLL+IH TA  KNLHPP C  ++CKG TAG
Sbjct: 75  NLVTLLGAFLCDTYFGRYKTVGFSIVASFLGLLLIHFTAVFKNLHPPHCIADVCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLMAGFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMM+S
Sbjct: 135 QMTFLMAGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMIS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           LTVIVYVQT+VSWALGLGIPA LMLIAC+LFFVGSKIYVK++ATGSPMTSVAQV VVAIK
Sbjct: 195 LTVIVYVQTNVSWALGLGIPAALMLIACILFFVGSKIYVKLEATGSPMTSVAQVLVVAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF+Y  PGSINSKLSYSDQFRFL+KAAIIT ED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFDYTTPGSINSKLSYSDQFRFLDKAAIITEEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC++RV+PIW++GVLYF AQ Q QTYAVFQALQSNRRLG+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLMRVLPIWMSGVLYFVAQLQLQTYAVFQALQSNRRLGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLS WLPIYDR +VP L K+T KE GIT LQRQGIGIFLA LT L+SA+VE RRR++
Sbjct: 375 AMLSLSFWLPIYDRIIVPFLQKVTKKEGGITTLQRQGIGIFLAILTMLLSAIVEDRRRII 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+PT+G EPRKGAIS++SASWLIPQL L GL+DGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPTVGIEPRKGAISSMSASWLIPQLLLYGLSDGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           MFFCA+A  SYL+GLLITVVHRMS  S  GDWLPEDLNKGRLDYFYYF+ GIE +NL YF
Sbjct: 495 MFFCAMAVASYLNGLLITVVHRMSRGSKYGDWLPEDLNKGRLDYFYYFVGGIELLNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           LVCA WYKYK APQN SEIH+ SKQ EK SV
Sbjct: 555 LVCAKWYKYKEAPQNASEIHMASKQPEKSSV 585

BLAST of MC06g0174 vs. ExPASy TrEMBL
Match: A0A6J1E193 (protein NRT1/ PTR FAMILY 2.11-like OS=Cucurbita moschata OX=3662 GN=LOC111429869 PE=3 SV=1)

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 476/571 (83.36%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 8   GETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGST 67
           GET+ HYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITA TLLNIFNG T
Sbjct: 15  GETQKHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATLLNIFNGCT 74

Query: 68  NLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAG 127
           NLVT++GAFLCDTYFGRYKT+GF+ VASFLGLL+IH TA  KNLHPP C  ++CKG TAG
Sbjct: 75  NLVTLLGAFLCDTYFGRYKTVGFSIVASFLGLLLIHFTALFKNLHPPHCIADVCKGPTAG 134

Query: 128 QMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVS 187
           QM FLMAGFGLM++GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMM+S
Sbjct: 135 QMTFLMAGFGLMIIGAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMIS 194

Query: 188 LTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIK 247
           LTVIVYVQT+VSWALGLGIPA LMLIAC+LFFVGSKIYVK++ATGSPMTSVAQV VVAIK
Sbjct: 195 LTVIVYVQTNVSWALGLGIPAALMLIACILFFVGSKIYVKLEATGSPMTSVAQVLVVAIK 254

Query: 248 KRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKL 307
           KRKLKQPDQPW+SLF+Y  PGSINSKLSYSDQFRFL+KAAIIT ED+IKEDGSAA+PW+L
Sbjct: 255 KRKLKQPDQPWLSLFDYTTPGSINSKLSYSDQFRFLDKAAIITEEDQIKEDGSAADPWRL 314

Query: 308 CSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVF 367
           CS+QQVEEVKC++RV+PIW+ GVL+F AQ Q QTY VFQALQSNRRLG+FTIPAASYTVF
Sbjct: 315 CSMQQVEEVKCLMRVLPIWMAGVLFFVAQLQLQTYVVFQALQSNRRLGNFTIPAASYTVF 374

Query: 368 SMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVV 427
           +MLSLS WLPIYDR +VP L K+T KE GIT LQRQGIGIFLA LT L+SA+VE RRR++
Sbjct: 375 AMLSLSFWLPIYDRIIVPFLQKVTKKEGGITTLQRQGIGIFLATLTMLLSAIVEDRRRII 434

Query: 428 ALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 487
           ALT+PT+G EPRKGAIS++SASWLIPQL L GL+DGFGAVSQLEFYYKQFPENMRSIGGS
Sbjct: 435 ALTKPTVGIEPRKGAISSMSASWLIPQLLLYGLSDGFGAVSQLEFYYKQFPENMRSIGGS 494

Query: 488 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 547
           MFFCA+A  SYL+GLLITVVHRMS  S  GDWLPEDLNKGRLDYFYYF+ GIE +NL YF
Sbjct: 495 MFFCAMAVASYLNGLLITVVHRMSRGSKYGDWLPEDLNKGRLDYFYYFVGGIELLNLCYF 554

Query: 548 LVCANWYKYKGAPQNPSEIHLISKQQEKMSV 577
           LVCA WYKYK APQN SEIHL SKQ EK SV
Sbjct: 555 LVCAKWYKYKEAPQNASEIHLASKQPEKNSV 585

BLAST of MC06g0174 vs. ExPASy TrEMBL
Match: A0A0A0LS88 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G008530 PE=3 SV=1)

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 471/557 (84.56%), Postives = 512/557 (91.92%), Query Frame = 0

Query: 22  FVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGSTNLVTIIGAFLCDTY 81
           F  GNETFEKLGAIGTLANLLIYLTSVFNMKSITA T+LNIFNGSTNLVT++GAFLCDTY
Sbjct: 4   FHSGNETFEKLGAIGTLANLLIYLTSVFNMKSITAATILNIFNGSTNLVTLVGAFLCDTY 63

Query: 82  FGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGELCKGATAGQMAFLMAGFGLMVV 141
           FGRYKTLGFA +ASFLGLLVIHLTAA+K LHPP C  +LCKG TAGQM FL+ GFGLM++
Sbjct: 64  FGRYKTLGFAIIASFLGLLVIHLTAAVKTLHPPHCIADLCKGPTAGQMTFLLFGFGLMII 123

Query: 142 GAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVSLTVIVYVQTSVSWA 201
           GAGGIRPCNLAFGADQFNP TEAGKKGINSFFNWYVFTYTFAMMVS+TVIVYVQT+VSWA
Sbjct: 124 GAGGIRPCNLAFGADQFNPNTEAGKKGINSFFNWYVFTYTFAMMVSITVIVYVQTNVSWA 183

Query: 202 LGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIKKRKLKQPDQPWVSL 261
           LGLGIPA+LMLIAC+LFFVGSKIYVKVKATGSPMTSVAQV VVAIKKRKLKQPDQPW+SL
Sbjct: 184 LGLGIPAILMLIACILFFVGSKIYVKVKATGSPMTSVAQVLVVAIKKRKLKQPDQPWLSL 243

Query: 262 FNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKLCSVQQVEEVKCVVR 321
           F Y PPGSINSKLSYSDQFRFL+KAAIITAED+IKEDGSAA+PWKLCS+QQVEEVKC+VR
Sbjct: 244 FEYTPPGSINSKLSYSDQFRFLDKAAIITAEDQIKEDGSAADPWKLCSMQQVEEVKCLVR 303

Query: 322 VIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGSFTIPAASYTVFSMLSLSIWLPIYDR 381
           V+P+W+ GVL+F  Q+QQ TYA+FQALQSNRR+G+FTIPAASYT+F+MLSLSIWLPIYDR
Sbjct: 304 VLPVWLAGVLFFATQAQQNTYAIFQALQSNRRIGNFTIPAASYTIFAMLSLSIWLPIYDR 363

Query: 382 FLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVVALTRPTLGFEPRKG 441
            +VP L K T KE GITILQR GIGIFL  +  L+S LVE RRR++ALT+P+LG EPRKG
Sbjct: 364 IVVPFLLKFTKKEGGITILQRLGIGIFLTTMAVLLSGLVEDRRRIIALTKPSLGIEPRKG 423

Query: 442 AISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGSMFFCAIAGGSYLSG 501
           AISA+SASWLIPQLTL GLADGFGAVSQLEFYYKQFPENMRSIGGSMFFCAIAGGSYL+G
Sbjct: 424 AISAMSASWLIPQLTLYGLADGFGAVSQLEFYYKQFPENMRSIGGSMFFCAIAGGSYLNG 483

Query: 502 LLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYFLVCANWYKYKGAPQ 561
           LLI VVHRMS  S SGDWLPEDLNKGRLDYFYYF+TGI  VNL YFL+C+ WYKYKGAPQ
Sbjct: 484 LLIIVVHRMSEGSKSGDWLPEDLNKGRLDYFYYFLTGIGLVNLCYFLICSKWYKYKGAPQ 543

Query: 562 NPSEIHLISKQQEKMSV 577
           N SEIHLISKQ EK SV
Sbjct: 544 NASEIHLISKQPEKNSV 560

BLAST of MC06g0174 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.1e-203
Identity = 345/583 (59.18%), Postives = 440/583 (75.47%), Query Frame = 0

Query: 1   AVQKIYDGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLL 60
           +V++    + ++ YRGWK MPF+IGNETFEKLG IGTL+NLL+YLT+VFN+KSITA T++
Sbjct: 31  SVEEDVQNQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATII 90

Query: 61  NIFNGSTNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRC---T 120
           N F+G+ N  T + AFLCDTYFGRYKTL  A +A FLG  VI LTAA+  LHP  C    
Sbjct: 91  NAFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAA 150

Query: 121 GELCKGATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYV 180
             +C G + GQ+AFL+ G G +VVGAGGIRPCNLAFGADQFNP +E+GK+GI+SFFNWY 
Sbjct: 151 DSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYF 210

Query: 181 FTYTFAMMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTS 240
           FT+TFA ++SLT++VYVQ++VSW +GL IPA+LM +ACL+FF G K+YVK+KA+GSP+  
Sbjct: 211 FTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAG 270

Query: 241 VAQVFVVAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKE 300
           +AQV  VAIKKR LK   QPW++L+NY PP   NSKL Y+DQFRFL+KAAI+T ED+++ 
Sbjct: 271 IAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQP 330

Query: 301 DGSAAEPWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGS- 360
           DG  A+PWKLC++QQVEEVKC+VRV+PIW    +Y+   +QQ TY VFQALQS+RRLGS 
Sbjct: 331 DGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSG 390

Query: 361 -FTIPAASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTAL 420
            F IPAA+Y VF M  +++++ +YDR LVP + ++T  ++GIT+LQR G GIF A  + +
Sbjct: 391 GFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLV 450

Query: 421 VSALVEARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYK 480
           V+  VE RRR  ALT+PTLG  PRKG IS++SA WLIPQL+LAG+A+ F A+ Q+EFYYK
Sbjct: 451 VAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYK 510

Query: 481 QFPENMRSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYF 540
           QFPENMRS  GS+F+      SYL   LI  VHR +  S  G+WL EDLNKGRLD FY+ 
Sbjct: 511 QFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFM 570

Query: 541 ITGIEFVNLGYFLVCANWYKYKGAPQNPSEIHL---ISKQQEK 575
           I GI  VN  YFLV + WY+YKG+    +       I KQQ+K
Sbjct: 571 IAGILAVNFAYFLVMSRWYRYKGSDDEVTTYETNENIIKQQDK 613

BLAST of MC06g0174 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 689.9 bits (1779), Expect = 1.8e-198
Identity = 337/577 (58.41%), Postives = 436/577 (75.56%), Query Frame = 0

Query: 2   VQKIYDGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLN 61
           V    + + ++ YRGWK MPF+IGNETFEKLG IGTL+NLL+YLTSVFN+KS TA T++N
Sbjct: 50  VDSFEEEQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIIN 109

Query: 62  IFNGSTNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGEL- 121
            F+G+ N  T I AFLCDTYFGRYKTL  A +A FLG  VI LTAA+ +LHP  C  ++ 
Sbjct: 110 AFSGTINFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKIS 169

Query: 122 CKGATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTY 181
           C+G + GQ+ FL+ G G +VVGAGGIRPCNLAFGADQFNP +E+GKKGINSFFNWY FT+
Sbjct: 170 CEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTF 229

Query: 182 TFAMMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQ 241
           TFA ++SLT +VY+Q++VSW +GL IP  LM +AC++FF G ++YVKVKA+GSP+  +A+
Sbjct: 230 TFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIAR 289

Query: 242 VFVVAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGS 301
           V   AIKKR LK   QPWV+L+N+IP    N+ L Y+DQFRFL+KAAI+T E+++  DG+
Sbjct: 290 VIAAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLNSDGT 349

Query: 302 AAEPWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLGS--FT 361
           A++PWKLC++QQVEEVKC+VRVIPIW    +Y+ A + Q TY VFQALQS+RRLGS  F 
Sbjct: 350 ASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFR 409

Query: 362 IPAASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSA 421
           IPAA+Y VF M  +++++  YDR LVP L ++T  E+GI++LQR G G   A ++ LVS 
Sbjct: 410 IPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSG 469

Query: 422 LVEARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFP 481
            +E RRR  ALT+PTLG  PR G IS++SA WLIPQLTLAG+A+ F A+ Q+EFYYKQFP
Sbjct: 470 FIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFP 529

Query: 482 ENMRSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITG 541
           ENM+S  GS+F+      SYL+  LI+ VHR +  S SG+WL EDLNK +LDYFY+ +TG
Sbjct: 530 ENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTG 589

Query: 542 IEFVNLGYFLVCANWYKYKGAPQNPSEIHLISKQQEK 575
           +  VN+ YFL+ A WY+YKG   N  +I  I   +E+
Sbjct: 590 LMVVNMAYFLLMARWYRYKGG--NDEDITEIETNEEE 624

BLAST of MC06g0174 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 677.2 bits (1746), Expect = 1.2e-194
Identity = 335/577 (58.06%), Postives = 437/577 (75.74%), Query Frame = 0

Query: 7   DGETELHYRGWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGS 66
           D E+++ YRGWK MPF+IGNETFEKLG +G+ +NL+IYLT+VFNMKSITA  ++NI+ G+
Sbjct: 13  DDESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGT 72

Query: 67  TNLVTIIGAFLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCT---GELCKG 126
           +N  TI+ AFLCD+YFGRYKTL FA +A FLG + + LTA +  LHP +C    G +C G
Sbjct: 73  SNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNG 132

Query: 127 ATAGQMAFLMAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFA 186
            + GQ+ FL     L+V+GAGGIRPCNL FGADQF+P T+ GK+GI SFFNWY FT+TFA
Sbjct: 133 PSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFA 192

Query: 187 MMVSLTVIVYVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFV 246
            MVSLT+IVYVQ++VSW++GL IPA+LML+ C++FF GSK+YVKVKA+GSP+ S+ +V V
Sbjct: 193 QMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIV 252

Query: 247 VAIKKRKLKQPDQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAE 306
           VAIKKR+LK P  P   L+NYI     NSKL +++QFRFL+K+AI T +D++ +DGS  +
Sbjct: 253 VAIKKRRLK-PVGP-NELYNYIASDFKNSKLGHTEQFRFLDKSAIQTQDDKLNKDGSPVD 312

Query: 307 PWKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRL--GSFTIPA 366
            WKLCS+QQVEEVKCV+RV+P+W++  L++ A  QQ TY +FQ+LQS+RRL  GSF IPA
Sbjct: 313 AWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPA 372

Query: 367 ASYTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVE 426
            SYTVF ML ++I++PIYDR LVP L K T ++ GIT LQR G G+FL   + +VSA+VE
Sbjct: 373 GSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVE 432

Query: 427 ARRRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENM 486
             RR VALT+PTLG  PRKGAIS++S  WLIPQL L G+AD    V Q+EFYYKQFPENM
Sbjct: 433 QYRRKVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENM 492

Query: 487 RSIGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEF 546
           RS  GS+++C I   SYLS  L++ VH  +     G WLPEDLNKGRL+YFY+ + G+  
Sbjct: 493 RSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMT 552

Query: 547 VNLGYFLVCANWYKYKGAPQNPSEIHLISKQQEKMSV 578
           +NL YFL+ ++WY+YK       ++   S + +K+SV
Sbjct: 553 LNLAYFLLVSHWYRYKDVVAKDKDMDKTSAEFDKVSV 587

BLAST of MC06g0174 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 488.4 bits (1256), Expect = 7.8e-138
Identity = 260/576 (45.14%), Postives = 371/576 (64.41%), Query Frame = 0

Query: 16  GWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGSTNLVTIIGA 75
           GW+A+ F++GNET E+LG+IG LAN ++YLT VF+++ + A  ++NI++G TNL  ++GA
Sbjct: 54  GWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGA 113

Query: 76  FLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTGE---LCKGATAGQMAFL 135
           ++ DTY GR+KT+ FA+ A+ LGL+ I LTA+   LHP  C  +    C G    Q+  L
Sbjct: 114 YISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVL 173

Query: 136 MAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVSLTVIV 195
           + G   + VG+GGIRPC++ FG DQF+  TE G KG+ SFFNWY  T+T  ++++ TV+V
Sbjct: 174 LLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVV 233

Query: 196 YVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIKKRKLK 255
           Y+Q  VSW +G  IP  LM +A ++FF G K YV VK  GS  + +AQV V A KKRKLK
Sbjct: 234 YIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLK 293

Query: 256 QP--DQPWVSLFNYIPPGSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEPWKLCSV 315
            P  D   V+ ++     S+ SKL  S+QFR L+KAA++   D +  +G  A+ W+LCSV
Sbjct: 294 LPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGD-LTPEGPPADKWRLCSV 353

Query: 316 QQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLG-SFTIPAASYTVFSM 375
           Q+VEEVKC++R++PIW  G++   A + Q T+ V QAL+ +R LG  F IPA S +V S+
Sbjct: 354 QEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISL 413

Query: 376 LSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEARRRVVAL 435
           L++ I+LP YDR  VP + ++T  +SGIT+LQR G GI  A  + +V+ +VE  RR+ ++
Sbjct: 414 LTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSI 473

Query: 436 TR--PTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRSIGGS 495
               PT         ++ +S  WL PQL L GL + F  + Q+EF+  QFPE+MRSI  S
Sbjct: 474 NAGDPT--------GMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 533

Query: 496 MFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVNLGYF 555
           +F  + AG SYLS  L+TVVH+ S      DWL ++LN G+LDYFYY I  +  VNL YF
Sbjct: 534 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYF 593

Query: 556 LVCANWYKYK-GAP-------QNPSEIHLISKQQEK 575
             CA  Y+YK G P       ++  ++ + SK+  K
Sbjct: 594 WYCARGYRYKVGLPIEDFEEDKSSDDVEMTSKKSMK 620

BLAST of MC06g0174 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.5e-125
Identity = 247/558 (44.27%), Postives = 347/558 (62.19%), Query Frame = 0

Query: 16  GWKAMPFVIGNETFEKLGAIGTLANLLIYLTSVFNMKSITAGTLLNIFNGSTNLVTIIGA 75
           GW+A+ F++GNET EKLG+IG  AN ++YL +VF+M+ + A  +  ++ G TN   ++GA
Sbjct: 17  GWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGA 76

Query: 76  FLCDTYFGRYKTLGFATVASFLGLLVIHLTAALKNLHPPRCTG---ELCKGATAGQMAFL 135
            + D Y GR+KT+ +A++ S LGL+ + LTA L  LHPP C     + C      Q+  L
Sbjct: 77  LISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGIL 136

Query: 136 MAGFGLMVVGAGGIRPCNLAFGADQFNP-TEAGKKGINSFFNWYVFTYTFAMMVSLTVIV 195
             G G + +G+GGIRPC++ FG DQF+  TE G KG+ SFFNWY  T T  ++ S TV+V
Sbjct: 137 FLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVV 196

Query: 196 YVQTSVSWALGLGIPALLMLIACLLFFVGSKIYVKVKATGSPMTSVAQVFVVAIKKRKLK 255
           Y+QT VSW +G  IP  LM  A +LFFVG + YV VK  GS  + +A+V V A KKR LK
Sbjct: 197 YLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLK 256

Query: 256 QPDQPWVSLFN------YIPP--GSINSKLSYSDQFRFLNKAAIITAEDRIKEDGSAAEP 315
                 +SL +      Y PP    + SKL  +DQF+FL+KAA+I   D +  +G  A  
Sbjct: 257 ------ISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANK 316

Query: 316 WKLCSVQQVEEVKCVVRVIPIWVTGVLYFTAQSQQQTYAVFQALQSNRRLG-SFTIPAAS 375
           W+LCS+Q+VEEVKC++RV+P+W  G++   A + Q T+ VFQA + +R +G  F IPAAS
Sbjct: 317 WRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAAS 376

Query: 376 YTVFSMLSLSIWLPIYDRFLVPLLHKLTAKESGITILQRQGIGIFLAALTALVSALVEAR 435
            TV S +++ IW+PIY+  LVP L ++  ++  +T+LQR GIGI  A L+   +  VE  
Sbjct: 377 ITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGV 436

Query: 436 RRVVALTRPTLGFEPRKGAISALSASWLIPQLTLAGLADGFGAVSQLEFYYKQFPENMRS 495
           RR    TR T         ++ +S  WL   L L GL + F  +  +EF+  QFPE+MRS
Sbjct: 437 RR----TRAT--------EMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRS 496

Query: 496 IGGSMFFCAIAGGSYLSGLLITVVHRMSTRSGSGDWLPEDLNKGRLDYFYYFITGIEFVN 555
           I  S+F  + A  +YLS LL+T VH++S      DWL +DL++G+LDYFYY I  +  VN
Sbjct: 497 IANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVN 552

Query: 556 LGYFLVCANWYKYKGAPQ 561
           L YF  CA+ Y+YK   Q
Sbjct: 557 LVYFWYCAHRYQYKAGSQ 552