MC06g0161 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0161
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	5-formyltetrahydrofolate cyclo-ligase
Location	MC06: 1298980 .. 1302829 (+)
RNA-Seq Expression	MC06g0161
Synteny	MC06g0161

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGGGTACACGCACGACTAACAAGGTTTATTTTTATTTTTGTTGACATAAAACAAGGTTTATTTAACATTCTACTTAAAAGAAAAGAAAATAAAAGAAAATGTTTATTTAACATTTATTCAGCAATCAGTACAAAATTCAAAATTTTGCAATCTCTGTGATTTTGGAACTGAAGGGTCCAACTCGTGGCGTTGGAAGCACGTTCTGTGTTGATTTGTTTGAGACAATTTGGATTTGATGATAAAATATCGGCTCATCCAAGGAAAGGATACAAATTCACGGGTTCATGGATTTCAATATTCGACCACTGCCAGTAGATCAAGGAACCAGCGAAGTCCAGCCGAAGTGCACGTGGGTCGGAGCCCAAGCGCCACAAGGTTGCCGATTTCCGAAGCCCCAATGCTCCGAAACTATAAACTACGAATGCCCATTGCAGCCAATTCACTGTTCACTCAACAACCACTTCATCAATTCTCGTCTCGCTCCTCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAATGAAGATCAATTGAAGGCCATATTCGAGCAGAAACGGACCCTTCGATCCAGCGTTCGAAAAGCCCTCAGAGCCATGGATCCCTCCCTCAGATCCCAGGAAGGTACCAATTGCTTGCTTATCTTCGTCCATTAACTTTGCTCCGATGGAAATGTCAGTTCGAGTAACATCGTTTTGATATGATTCTGAATCAATGTTTCCCCTCTTTCATTGGCCTTTCTACTTGTTTCTTAGACAATGCGATTCAGAATATTATTTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTGTGTGCTTATGTTAGTTGCAGTGCCTTGAGAGAAGTTGATACGTCGAGACTGTTATCAGAGATTCTGCAACACCAACCAAAAGGTGTGTTCTTATTAGCACTTATTACATATAAGATATCTGAAATTCCAAGGGATCCATTGGGAAACTTGACGCAATCAAATTAGTAAGACTCAACAAAAGATTTTATGTTTAAAAGTTTACATTGAATAAGAAATACACACATGATAAGTATTTCAGTGAAGTTCTTTAGTTAAATTTAACTAATTAATGTGATAAAATACGAGTGATTGATTGATGTATTGGTTCCGGATGCAGAATACATATGTGGAAGTGTTGTTACTTGTGATGCTAGCAACTTCTATTTTCTCTTTTGAACAAGAAACTGGTAGTTATTGATTCTGTCATGCTTCTAGCTGAGCTTTGTTAATGTTGACATTTTGCCTTTTTTTTAAAATTTTTTTTATCTGAAGATGGTTATTCGAAGAAGATTTATGTCCCACGTGTGGAGGACAAGAACAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAACTCAATGAATATCTTAGAACCAGCTCCAGTGGACGGTGATGGAAATGAACGTGAAGATGGTAACTAGACACTCTACCCCTCGTTTCGCTCTCTCTCTCTCTCTCCCTCTCTCTCGTCTTTCATTTTCTATCAGTCCCCAGCTTAAAGAACATGCTTGTGATTTTTATGATTAATGTACAATCTACTCTATATTGCATTGGGAAAGTGATATGCACTTGACTTATATAGCACTAGCATAGAGATAGCTTCTTAAAATATAACCATTTATATTCATAACATCACTGATATATATTCTTTCATGCTTAAAAAGGAATAGTTTTTCATTAAAGAAAGAACATTACTGATATACTATCTTTTTTGGTTTGGTCTCCCCTAATGCAATTGGATGGCTTTGCAAATAACTCCAGATCATTGTCTTCTTACCACTTGGGACCAGAAATATTTTCTGTTTATCACCAGCAAAAAGCCTGGTTATTCTTTATTGTCTTCGATGGTGTCAATAGATCTATTAATGTCCAATGTGAGCCTGTTTAAAAAAGAAAAAAAAAATTCATTGTGAGCCAAAACTTAACTATCCGCGATTTGTATGTTTGATAGATTCCATAGAATCATTGCCCTCTGAATACTTGTCTTTAGTGCTTTTCTGTATTTTCTTCGAGCTTAAAGACGGGTCATGGAACTGTTATTTCATTCTTCTAATGTTTTTTGAAACCCAGAAGTCTTTAACTTATTTTGTTGAACTCCTTTTATAGCCTTGTTAAGTTTTCCTGGAAGACTCATTCTACTGATATCATATAAACCACGGCTGTTTAAGCTTAGAAATAATTGGTCTAACATAGTTGTTTATGATAGAAATGAAGAGCACAAATAAGGAAGGTGTTCAGCATACCTCGACCATTGAACTAGGCTGTACCATGACCATCTTATCTGTTATCCACATGCCAATAGAAAAACATGAAATTTTATGTCATTTTCCCCTCAGGGTGGTAGTGCTTAGCCATAAGTCGGCTTCTGCACTGCATATATTATAACAGAACTCTTTTGGTGATATCTACCAAATGTTATCGTTAACCATATTATCTCTTTTCTCTTCTTCTTTTTCTATCTTCGAATAAAAGAAATGGTTTAGCATAATCTCTCTCTGCATCTTGAATGTAACAACGCTCATATTTAAACATCCGTCTTTTGCAGTTATGCAGACAAATGACCCCATTGATTTATTCCTCTTACCAGGTGAGATTTTCATTACCTAATTGTTGATTGTTCCAGTTGATTTGTAGTTATAAGCCAACATAAAATCACTTATCAATATGTTGATGATTGCTACCAGGTCTAGCATTTGATAAATCAGGAAGGCGCCTAGGCCGAGGAGGAGGGTATGGATTTGCATTTAGCCTTTTTGAGGTTTCTTTTTGCCACTGTTATTACTGAACTTCTAACATATTTGGAGCTGATTTGTGAAATTGAAACAATCTGTATAACCTACTACGTCCTACTCCAAACTAATCTTACCCTATAATTGTTTCGAAAATAGGTACTATGATACTTTCCTAAAGAACTACCAAGAGCTTGCGAAAGTGCGGAATTGGAAGCAGCCCCTCCTTGGTATGTGTTTAAACTCTCTCTTGTAGTAGAACCTTGCCTTAGTTTCTTTTTTTGGCTTACGTTGTTCTCTGCGACCTCACTTAACTCAGTTGGCATGATTTTGCAGTTGCACTGTCCTATTCAGTGCAGATAATTGATGAAGGAGTTATACCACTCACTCCAAATGACGTTTCAGTAGATGCTCTTGTATCACCATCTGGTGAGATTCCCACCAGCTCGGCTGGACTGGACAAGTAATTTCTTTCATCCGAAAAGTCTAAGGATCGCTAGTTGTTCCAATTTTTTCTCTGGTTTCTTACTTCCTACTCGTCGTATCTTCGCAGAATAAGAGTGTGATATTAGACTCAGAGAATCAATCCTGAACGATGCCTTATTTGATGAAATCCTTGACAAGCTGGAGCTCGGAATCCTCGAAGCTGTTACATTATGCGTACGCCTTTCCCCTCTCTGCTTTTCAGATAAGGCATCACGAGGAAGTTATGGTTCCTCTAGCTTCCAACTGTGATATAACTCTGAATGACATGTAAGTTAGTTGATTTGTATACGCATGCTTTAGGCAGCATCGAGGAAACTATGATGTATTATTGACTATAAAAAGACTTTTTTTTGGTTGTTTTCAAACAAATAACTGGTCATCTGCAAATACTTTCATCATCTGGAATAAACGCGTTGGTGGTGTTCAGAAAATGAAGGTGCACTTGCTCTTAAGAGTTGTTGGTCATTTGTAGACAAAGGGGTCTTTGATGCTTATGTTTAAAAGTTCTTTAAGTTTCCTTTAGCTTTAAAGTTCAAAGTTAGTTAGTAATGCGTCGGCCTATCAGTCCTTATCCATGTTCCTGTCTGCTGGTAAAAG

mRNA sequence

TGGGGGTACACGCACGACTAACAAGGTTTATTTTTATTTTTGTTGACATAAAACAAGGTTTATTTAACATTCTACTTAAAAGAAAAGAAAATAAAAGAAAATGTTTATTTAACATTTATTCAGCAATCAGTACAAAATTCAAAATTTTGCAATCTCTGTGATTTTGGAACTGAAGGGTCCAACTCGTGGCGTTGGAAGCACGTTCTGTGTTGATTTGTTTGAGACAATTTGGATTTGATGATAAAATATCGGCTCATCCAAGGAAAGGATACAAATTCACGGGTTCATGGATTTCAATATTCGACCACTGCCAGTAGATCAAGGAACCAGCGAAGTCCAGCCGAAGTGCACGTGGGTCGGAGCCCAAGCGCCACAAGGTTGCCGATTTCCGAAGCCCCAATGCTCCGAAACTATAAACTACGAATGCCCATTGCAGCCAATTCACTGTTCACTCAACAACCACTTCATCAATTCTCGTCTCGCTCCTCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAATGAAGATCAATTGAAGGCCATATTCGAGCAGAAACGGACCCTTCGATCCAGCGTTCGAAAAGCCCTCAGAGCCATGGATCCCTCCCTCAGATCCCAGGAAGACAATGCGATTCAGAATATTATTTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTGTGTGCTTATGTTAGTTGCAGTGCCTTGAGAGAAGTTGATACGTCGAGACTGTTATCAGAGATTCTGCAACACCAACCAAAAGATGGTTATTCGAAGAAGATTTATGTCCCACGTGTGGAGGACAAGAACAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAACTCAATGAATATCTTAGAACCAGCTCCAGTGGACGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAATGACCCCATTGATTTATTCCTCTTACCAGGTCTAGCATTTGATAAATCAGGAAGGCGCCTAGGCCGAGGAGGAGGGTACTATGATACTTTCCTAAAGAACTACCAAGAGCTTGCGAAAGTGCGGAATTGGAAGCAGCCCCTCCTTGTTGCACTGTCCTATTCAGTGCAGATAATTGATGAAGGAGTTATACCACTCACTCCAAATGACGTTTCAGTAGATGCTCTTGTATCACCATCTGGTGAGATTCCCACCAGCTCGGCTGGACTGGACAAAATAAGAGTGTGATATTAGACTCAGAGAATCAATCCTGAACGATGCCTTATTTGATGAAATCCTTGACAAGCTGGAGCTCGGAATCCTCGAAGCTGTTACATTATGCGTACGCCTTTCCCCTCTCTGCTTTTCAGATAAGGCATCACGAGGAAGTTATGGTTCCTCTAGCTTCCAACTGTGATATAACTCTGAATGACATGTAAGTTAGTTGATTTGTATACGCATGCTTTAGGCAGCATCGAGGAAACTATGATGTATTATTGACTATAAAAAGACTTTTTTTTGGTTGTTTTCAAACAAATAACTGGTCATCTGCAAATACTTTCATCATCTGGAATAAACGCGTTGGTGGTGTTCAGAAAATGAAGGTGCACTTGCTCTTAAGAGTTGTTGGTCATTTGTAGACAAAGGGGTCTTTGATGCTTATGTTTAAAAGTTCTTTAAGTTTCCTTTAGCTTTAAAGTTCAAAGTTAGTTAGTAATGCGTCGGCCTATCAGTCCTTATCCATGTTCCTGTCTGCTGGTAAAAG

Coding sequence (CDS)

ATGATAAAATATCGGCTCATCCAAGGAAAGGATACAAATTCACGGGTTCATGGATTTCAATATTCGACCACTGCCAGTAGATCAAGGAACCAGCGAAGTCCAGCCGAAGTGCACGTGGGTCGGAGCCCAAGCGCCACAAGGTTGCCGATTTCCGAAGCCCCAATGCTCCGAAACTATAAACTACGAATGCCCATTGCAGCCAATTCACTGTTCACTCAACAACCACTTCATCAATTCTCGTCTCGCTCCTCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAATGAAGATCAATTGAAGGCCATATTCGAGCAGAAACGGACCCTTCGATCCAGCGTTCGAAAAGCCCTCAGAGCCATGGATCCCTCCCTCAGATCCCAGGAAGACAATGCGATTCAGAATATTATTTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTGTGTGCTTATGTTAGTTGCAGTGCCTTGAGAGAAGTTGATACGTCGAGACTGTTATCAGAGATTCTGCAACACCAACCAAAAGATGGTTATTCGAAGAAGATTTATGTCCCACGTGTGGAGGACAAGAACAGTCACATGCGGATGTTCAATATTTCACGTATGGATGATCTTATTGCAAACTCAATGAATATCTTAGAACCAGCTCCAGTGGACGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAATGACCCCATTGATTTATTCCTCTTACCAGGTCTAGCATTTGATAAATCAGGAAGGCGCCTAGGCCGAGGAGGAGGGTACTATGATACTTTCCTAAAGAACTACCAAGAGCTTGCGAAAGTGCGGAATTGGAAGCAGCCCCTCCTTGTTGCACTGTCCTATTCAGTGCAGATAATTGATGAAGGAGTTATACCACTCACTCCAAATGACGTTTCAGTAGATGCTCTTGTATCACCATCTGGTGAGATTCCCACCAGCTCGGCTGGACTGGACAAAATAAGAGTGTGA

Protein sequence

MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYKLRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV

Homology

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BLAST of MC06g0161 vs. ExPASy Swiss-Prot
Match: Q8L539 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=5FCL PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.6e-86
Identity = 160/258 (62.02%), Postives = 206/258 (79.84%), Query Frame = 0


Query: 62  RMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKAL 121
           R PI        +P+ + S  +   P +A + +SK  N+++L +IF+QKR +RS+VRK+L
Sbjct: 16  RSPIFFFPKIPTRPVFRLSPAT--RPIVAMSTTSK--NQEELDSIFKQKRVVRSTVRKSL 75

Query: 122 RAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKDG 181
           +AMDPSLR+Q+D AIQ  +LEAPWFKS + LCAY+SC +L EVDTS++LSEILQH P   
Sbjct: 76  KAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQH-PDSN 135

Query: 182 YSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPIDL 241
             KK+YVP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q ++PIDL
Sbjct: 136 TQKKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDL 195

Query: 242 FLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIPL 301
           F+LPGLAFD+ GRRLGRGGGYYDTFLK YQ+ AK + W+ PL+VALSYS QI+++G IP+
Sbjct: 196 FILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPV 255

Query: 302 TPNDVSVDALVSPSGEIP 320
           TPNDV +DALV+PSG +P
Sbjct: 256 TPNDVLIDALVTPSGVVP 268

[Close]

BLAST of MC06g0161 vs. ExPASy Swiss-Prot
Match: P49914 (5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 1.7e-20
Identity = 65/211 (30.81%), Postives = 113/211 (53.55%), Query Frame = 0


Query: 105 AIFEQKRTLRSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREV 164
           A+   KR+LR  +++ LRAM    R ++   +   ++    ++ S+R+  ++S     E+
Sbjct: 5   AVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQ--DEI 64

Query: 165 DTSRLLSEILQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAP 224
           +T  ++ +I Q        K  ++PR   +++HM M  I   ++   L   S NI +P  
Sbjct: 65  ETEEIIKDIFQR------GKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQP-- 124

Query: 225 VDGDGNEREDVMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQ 284
             G+G+ RE+ + T   +DL  +PGL FDK G RLGRG GYYD +LK   +  +V+    
Sbjct: 125 --GEGDVREEALSTGG-LDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVK---- 184

Query: 285 PLLVALSYSVQIIDEGVIPLTPNDVSVDALV 313
           P  +AL++  QI  +  +P+  ND+ VD ++
Sbjct: 185 PYTLALAFKEQICLQ--VPVNENDMKVDEVL 196

[Close]

BLAST of MC06g0161 vs. ExPASy Swiss-Prot
Match: Q9D110 (5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 3.1e-19
Identity = 64/206 (31.07%), Postives = 115/206 (55.83%), Query Frame = 0


Query: 110 KRTLRSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRL 169
           KR LR+ +++ LRA+    R ++   +   ++    +++S+R+  ++S     EV+T  +
Sbjct: 10  KRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHNQYQNSKRISIFLSMQ--DEVETEVI 69

Query: 170 LSEILQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAPVDGDG 229
           + +I +        K  ++PR + +++HM M  ++  ++   L   S NI +P    G+G
Sbjct: 70  IKDIFKQ------GKICFIPRYQFQSNHMDMVRLTSSEEIALLPKTSWNIHQP----GEG 129

Query: 230 NEREDVMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVA 289
           + RE+ + T   +DL  LPGL FDK G RLGRG GYYDT+LK   +  +V+    P  +A
Sbjct: 130 DVREEALSTGG-LDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEVK----PYTMA 189

Query: 290 LSYSVQIIDEGVIPLTPNDVSVDALV 313
           L++  QI  +  IP+  +D+ VD ++
Sbjct: 190 LAFKEQICPQ--IPVDEHDMKVDEVL 196

[Close]

BLAST of MC06g0161 vs. ExPASy Swiss-Prot
Match: P80405 (5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-18
Identity = 61/211 (28.91%), Postives = 115/211 (54.50%), Query Frame = 0


Query: 105 AIFEQKRTLRSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREV 164
           A+   KR+LR+ +++ LRA+    R +    +   ++    ++ SQR+  ++S     E+
Sbjct: 5   AVSGAKRSLRAELKQRLRAISAEERLRCQRLLTQKVIAHRQYQKSQRISIFLSMP--DEI 64

Query: 165 DTSRLLSEILQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIA---NSMNILEPAP 224
           +T  ++ +I Q        K  ++PR   +++HM M  ++  D++ +    S NI +P+ 
Sbjct: 65  ETEEIIKDIFQQ------GKVCFIPRYRLQSNHMDMVKLASADEISSLPKTSWNIHQPS- 124

Query: 225 VDGDGNEREDVMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQ 284
              + + RE+ + T   +DL  +PGL FD++G RLGRG GYYDT+L+   +    +   +
Sbjct: 125 ---ESDTREEALATGG-LDLIFMPGLGFDRNGNRLGRGRGYYDTYLQRCLQ----QQGAK 184

Query: 285 PLLVALSYSVQIIDEGVIPLTPNDVSVDALV 313
           P  +AL++  QI  +  +P+   DVSVD ++
Sbjct: 185 PYTIALAFREQICPQ--VPVDDTDVSVDEVL 196

[Close]

BLAST of MC06g0161 vs. ExPASy Swiss-Prot
Match: Q9XWE6 (Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239 GN=Y106G6E.4 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-14
Identity = 60/213 (28.17%), Postives = 99/213 (46.48%), Query Frame = 0


Query: 103 LKAIFEQKRTLRSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALR 162
           + AI E K  LR  ++  L  +      ++  A+   I+E+ WF+ S+RL  YVS S   
Sbjct: 1   MSAIKEVKSELRQFMKTLLGKISKEETQRQTEAVFEKIIESKWFQESKRLSVYVSTSG-- 60

Query: 163 EVDTSRLLSEILQHQPKDGYSKKIYVPRVEDKNSHMRMFNI---SRMDDLIANSMNILEP 222
           E+ T  ++ + L+        K++++P+    ++ M M  +   +  D+L +    I +P
Sbjct: 61  EIQTDSIIQKALE------MGKEVFIPQFTKGSTAMDMVRVPDQTAFDNLPSTLWGIRQP 120

Query: 223 APVDGDGNEREDVMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNW 282
            P       +        P+DL L PG+AF   G R G G GYYD F   +    K    
Sbjct: 121 EP-----KWKWQSYHETGPLDLILAPGVAFSPYGLRCGHGKGYYDRFFSTHH---KHFPE 180

Query: 283 KQPLLVALSYSVQIIDEGVIPLTPNDVSVDALV 313
             P  + L+   QII  G IP++  DV +D ++
Sbjct: 181 NSPKKIGLALREQII--GTIPISETDVELDEVI 195

[Close]

BLAST of MC06g0161 vs. NCBI nr
Match: XP_022135492.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 639 bits (1647), Expect = 1.27e-230
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK
Sbjct: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60

Query: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120
           LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA
Sbjct: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120

Query: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180
           LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD
Sbjct: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180

Query: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240
           GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID
Sbjct: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240

Query: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300
           LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP
Sbjct: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300

Query: 301 LTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           LTPNDVSVDALVSPSGEIPTSSAGLDKIRV
Sbjct: 301 LTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330

[Close]

BLAST of MC06g0161 vs. NCBI nr
Match: XP_022135493.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 610 bits (1574), Expect = 1.01e-219
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK
Sbjct: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60

Query: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120
           LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA
Sbjct: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120

Query: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180
           LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD
Sbjct: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180

Query: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240
           GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID
Sbjct: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240

Query: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300
           LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP
Sbjct: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300

Query: 301 LTPNDVSVDALVSPS 315
           LTPNDVSVDALVSPS
Sbjct: 301 LTPNDVSVDALVSPS 315

[Close]

BLAST of MC06g0161 vs. NCBI nr
Match: XP_022988398.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 528 bits (1361), Expect = 5.95e-187
Identity = 279/337 (82.79%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIK +L+QG  TN  VHG Q STTASR  N RSPA VHVGRSP A R  ISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  LRMPIAANSLFTQQP-------LHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
           L +PIAA+SLF + P       L   S  SSGSPKMA NE  KPENE+QLKA+FEQKR +
Sbjct: 61  L-LPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPSLRSQEDNAIQNI+LEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQH PKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300
           QT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 IDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           +DEG+IPLTPNDV VD LVSPSG IP SSAGLDK++V
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of MC06g0161 vs. NCBI nr
Match: XP_004138292.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 523 bits (1348), Expect = 5.88e-185
Identity = 276/337 (81.90%), Postives = 293/337 (86.94%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           M+KY+L+QG  TN  V+GFQ STT  R  NQ SPA VH+GRSP   +  ISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  LRMPIAANSLFT-------QQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
             +PIAANSLF        QQ L   S  SSG PKMA+NES KPENEDQLK IF+QKR L
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPS RSQEDN IQ+I+LEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQH PKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300
           QT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 IDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           +DEG+IPLTPNDV VDALVSPSG IP SSAGLDK++V
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

[Close]

BLAST of MC06g0161 vs. NCBI nr
Match: XP_022988397.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 521 bits (1343), Expect = 3.52e-184
Identity = 278/339 (82.01%), Postives = 295/339 (87.02%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIK +L+QG  TN  VHG Q STTASR  N RSPA VHVGRSP A R  ISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  LRMPIAANSLFTQQP-------LHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
           L +PIAA+SLF + P       L   S  SSGSPKMA NE  KPENE+QLKA+FEQKR +
Sbjct: 61  L-LPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPSLRSQEDNAIQNI+LEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED-- 240
           LQH PKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED  
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGN 240

Query: 241 VMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300
            MQT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV
Sbjct: 241 FMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300

Query: 301 QIIDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           QI+DEG+IPLTPNDV VD LVSPSG IP SSAGLDK++V
Sbjct: 301 QIMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

[Close]

BLAST of MC06g0161 vs. ExPASy TrEMBL
Match: A0A6J1C2U9 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007435 PE=4 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 6.16e-231
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK
Sbjct: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60

Query: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120
           LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA
Sbjct: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120

Query: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180
           LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD
Sbjct: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180

Query: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240
           GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID
Sbjct: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240

Query: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300
           LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP
Sbjct: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300

Query: 301 LTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           LTPNDVSVDALVSPSGEIPTSSAGLDKIRV
Sbjct: 301 LTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330

[Close]

BLAST of MC06g0161 vs. ExPASy TrEMBL
Match: A0A6J1C4Z1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007435 PE=4 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 4.89e-220
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK
Sbjct: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60

Query: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120
           LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA
Sbjct: 61  LRMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKA 120

Query: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180
           LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD
Sbjct: 121 LRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKD 180

Query: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240
           GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID
Sbjct: 181 GYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPID 240

Query: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300
           LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP
Sbjct: 241 LFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIP 300

Query: 301 LTPNDVSVDALVSPS 315
           LTPNDVSVDALVSPS
Sbjct: 301 LTPNDVSVDALVSPS 315

[Close]

BLAST of MC06g0161 vs. ExPASy TrEMBL
Match: A0A6J1JM66 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 528 bits (1361), Expect = 2.88e-187
Identity = 279/337 (82.79%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIK +L+QG  TN  VHG Q STTASR  N RSPA VHVGRSP A R  ISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  LRMPIAANSLFTQQP-------LHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
           L +PIAA+SLF + P       L   S  SSGSPKMA NE  KPENE+QLKA+FEQKR +
Sbjct: 61  L-LPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPSLRSQEDNAIQNI+LEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQH PKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300
           QT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 IDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           +DEG+IPLTPNDV VD LVSPSG IP SSAGLDK++V
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of MC06g0161 vs. ExPASy TrEMBL
Match: A0A0A0LRK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 2.85e-185
Identity = 276/337 (81.90%), Postives = 293/337 (86.94%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           M+KY+L+QG  TN  V+GFQ STT  R  NQ SPA VH+GRSP   +  ISEAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  LRMPIAANSLFT-------QQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
             +PIAANSLF        QQ L   S  SSG PKMA+NES KPENEDQLK IF+QKR L
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPS RSQEDN IQ+I+LEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQH PKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300
           QT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 IDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           +DEG+IPLTPNDV VDALVSPSG IP SSAGLDK++V
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

[Close]

BLAST of MC06g0161 vs. ExPASy TrEMBL
Match: A0A6J1JCX7 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 521 bits (1343), Expect = 1.70e-184
Identity = 278/339 (82.01%), Postives = 295/339 (87.02%), Query Frame = 0


Query: 1   MIKYRLIQGKDTNSRVHGFQYSTTASRSRNQRSPAEVHVGRSPSATRLPISEAPMLRNYK 60
           MIK +L+QG  TN  VHG Q STTASR  N RSPA VHVGRSP A R  ISE+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  LRMPIAANSLFTQQP-------LHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTL 120
           L +PIAA+SLF + P       L   S  SSGSPKMA NE  KPENE+QLKA+FEQKR +
Sbjct: 61  L-LPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALRAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180
           RSSVRKAL+AMDPSLRSQEDNAIQNI+LEAPWFKSSQRLCAYVSCSALREVDTSR+LSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQHQPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED-- 240
           LQH PKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED  
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGN 240

Query: 241 VMQTNDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300
            MQT DPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV
Sbjct: 241 FMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300

Query: 301 QIIDEGVIPLTPNDVSVDALVSPSGEIPTSSAGLDKIRV 330
           QI+DEG+IPLTPNDV VD LVSPSG IP SSAGLDK++V
Sbjct: 301 QIMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

[Close]

BLAST of MC06g0161 vs. TAIR 10
Match: AT5G13050.1 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 318.9 bits (816), Expect = 4.7e-87
Identity = 160/258 (62.02%), Postives = 206/258 (79.84%), Query Frame = 0


Query: 62  RMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKAL 121
           R PI        +P+ + S  +   P +A + +SK  N+++L +IF+QKR +RS+VRK+L
Sbjct: 16  RSPIFFFPKIPTRPVFRLSPAT--RPIVAMSTTSK--NQEELDSIFKQKRVVRSTVRKSL 75

Query: 122 RAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKDG 181
           +AMDPSLR+Q+D AIQ  +LEAPWFKS + LCAY+SC +L EVDTS++LSEILQH P   
Sbjct: 76  KAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQH-PDSN 135

Query: 182 YSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPIDL 241
             KK+YVP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q ++PIDL
Sbjct: 136 TQKKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDL 195

Query: 242 FLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIIDEGVIPL 301
           F+LPGLAFD+ GRRLGRGGGYYDTFLK YQ+ AK + W+ PL+VALSYS QI+++G IP+
Sbjct: 196 FILPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPV 255

Query: 302 TPNDVSVDALVSPSGEIP 320
           TPNDV +DALV+PSG +P
Sbjct: 256 TPNDVLIDALVTPSGVVP 268

[Close]

BLAST of MC06g0161 vs. TAIR 10
Match: AT5G13050.2 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 232.6 bits (592), Expect = 4.4e-61
Identity = 120/198 (60.61%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 62  RMPIAANSLFTQQPLHQFSSRSSGSPKMASNESSKPENEDQLKAIFEQKRTLRSSVRKAL 121
           R PI        +P+ + S  +   P +A + +SK  N+++L +IF+QKR +RS+VRK+L
Sbjct: 16  RSPIFFFPKIPTRPVFRLSPAT--RPIVAMSTTSK--NQEELDSIFKQKRVVRSTVRKSL 75

Query: 122 RAMDPSLRSQEDNAIQNIILEAPWFKSSQRLCAYVSCSALREVDTSRLLSEILQHQPKDG 181
           +AMDPSLR+Q+D AIQ  +LEAPWFKS + LCAY+SC +L EVDTS++LSEILQH P   
Sbjct: 76  KAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQH-PDSN 135

Query: 182 YSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTNDPIDL 241
             KK+YVP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q ++PIDL
Sbjct: 136 TQKKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDL 195

Query: 242 FLLPGLAFDKSGRRLGRG 260
           F+LPGLAFD+ GRRLGRG
Sbjct: 196 FILPGLAFDRCGRRLGRG 208

The following BLAST results are available for this feature:

BLAST of MC06g0161 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L539	6.6e-86	62.02	5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=...	[more]
P49914	1.7e-20	30.81	5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2	[more]
Q9D110	3.1e-19	31.07	5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=...	[more]
P80405	1.2e-18	28.91	5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS ...	[more]
Q9XWE6	1.4e-14	28.17	Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239...	[more]

BLAST of MC06g0161 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135492.1	1.27e-230	100.00	5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Momordica chara...	[more]
XP_022135493.1	1.01e-219	100.00	5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X2 [Momordica chara...	[more]
XP_022988398.1	5.95e-187	82.79	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita ...	[more]
XP_004138292.1	5.88e-185	81.90	5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus]	[more]
XP_022988397.1	3.52e-184	82.01	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 [Cucurbita ...	[more]

BLAST of MC06g0161 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2U9	6.16e-231	100.00	5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Momordica cha...	[more]
A0A6J1C4Z1	4.89e-220	100.00	5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X2 OS=Momordica cha...	[more]
A0A6J1JM66	2.88e-187	82.79	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit...	[more]
A0A0A0LRK4	2.85e-185	81.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1	[more]
A0A6J1JCX7	1.70e-184	82.01	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbit...	[more]

BLAST of MC06g0161 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13050.1	4.7e-87	62.02	5-formyltetrahydrofolate cycloligase	[more]
AT5G13050.2	4.4e-61	60.61	5-formyltetrahydrofolate cycloligase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024185	5-formyltetrahydrofolate cyclo-ligase-like domain superfamily	GENE3D	3.40.50.10420		coord: 102..317 e-value: 2.2E-55 score: 189.3
IPR002698	5-formyltetrahydrofolate cyclo-ligase	TIGRFAM	TIGR02727	TIGR02727	coord: 110..313 e-value: 5.3E-31 score: 105.9
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PFAM	PF01812	5-FTHF_cyc-lig	coord: 110..314 e-value: 2.6E-34 score: 118.7
None	No IPR available	PIRSR	PIRSR006806-1	PIRSR006806-1	coord: 108..223 e-value: 5.1E-12 score: 43.9 coord: 237..313 e-value: 5.2E-18 score: 63.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..101
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..37
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..46
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..93
None	No IPR available	PANTHER	PTHR23407	ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE	coord: 77..327
None	No IPR available	PANTHER	PTHR23407:SF10	5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE, MITOCHONDRIAL-LIKE ISOFORM X1	coord: 77..327
IPR037171	NagB/RpiA transferase-like	SUPERFAMILY	100950	NagB/RpiA/CoA transferase-like	coord: 109..320

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0161.1	MC06g0161.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0031213	RSF complex
molecular_function	GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding