MC06g0129 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0129 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 690.6 bits (1781), Expect = 1.5e-197
Identity = 356/596 (59.73%), Postives = 436/596 (73.15%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT KASL RYWN  +  Q P+ +FL SKASPL+AVDSA  +  A+    S+ +RF   
Sbjct: 35  NPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAAN--SLPTRFPDF 94

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           CS+ANLFCF D    +      D  F++Y  +NF NYG  R GGADSFKNYS   N    
Sbjct: 95  CSAANLFCFPDLGASLE-KHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTD 154

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SF+RY  N++ H + FT Y E+ NV    F SY     GG+G+F NY+  VN P  RFTA
Sbjct: 155 SFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTA 214

Query: 197 YDLDSRAHKLSFTGYGNETNSGS-QSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGE 256
           Y         +F  Y +E N+G+ QSFTSYGKNGNGVP EF+SY   SN++ S F+ YGE
Sbjct: 215 YSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGE 274

Query: 257 AGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNS 316
           +G +AND+F  YG  GN P +NF +YG  GN+ +DTF+NYR+ ANVGDDSF SYAK+SNS
Sbjct: 275 SGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNS 334

Query: 317 GRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFT 376
            + NF NYG+SFN G+++F  YG+G+ G + + FK Y+ + +F  YAK GV+F++Y+  T
Sbjct: 335 EKVNFVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVST 394

Query: 377 T---ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSS 436
           T     G G  VN+W+E GKFFRES LK+G V+ MPDI+DKMP RSFLPR I+  LPFS+
Sbjct: 395 TTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFST 454

Query: 437 SKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNV 496
           SK+ E+K IFHA E STM  +ITDA+ ECER PS  ETKRCVGSAEDMIDFATSVLGR+V
Sbjct: 455 SKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSV 514

Query: 497 VARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADIL 556
           V R T NV+GS E V IG V GINGG+LT++VSCHQSLYPYLLYYCHSVPKVRVYEAD+L
Sbjct: 515 VLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLL 574

Query: 557 SLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 609
            L +K+KINHG+AICHMDTSSWGP+HGAFLALGS PGRIEVCHWIFEND++W++AD
Sbjct: 575 ELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of MC06g0129 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 670.2 bits (1728), Expect = 2.2e-191
Identity = 346/596 (58.05%), Postives = 427/596 (71.64%), Query Frame = 0

Query: 14  PALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRF 73
           P +NPFT KASL RYWN  + +Q P   FL SKASPL+AV SA  A  AS    ++  R 
Sbjct: 33  PEINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASEN--ALPDRL 92

Query: 74  ESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNS 133
              CS+ANLFCF D    +    + D  F++Y  RNF NYG    GGADSFK YS   N+
Sbjct: 93  PDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNA 152

Query: 134 ADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLR 193
              SF+RY  +++ H + FT YA++ NV    F SY   + GGSG+F NY+  VN P  R
Sbjct: 153 VTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTR 212

Query: 194 FTAYDLDSRAHKLSFTGYGNETNSG-SQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTG 253
           F AY  +      +F  Y +E NSG  QSFTSYGKNGNG P EFSSY   SN++ S F+ 
Sbjct: 213 FKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSN 272

Query: 254 YGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKN 313
           YGE G +AND+FK YG  GN P +NF+SYG  GN+ +DTF NYR+ ANVGDDSF SYAKN
Sbjct: 273 YGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKN 332

Query: 314 SNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYS 373
           SN  + +F NYG+S N G++SF  YG+G+ G   + FK Y+ + +F  Y KTGV F++Y+
Sbjct: 333 SNFEKVDFVNYGKSINPGSESFSGYGKGAEG-NKIDFKTYTQNSTFKDYTKTGVEFAKYN 392

Query: 374 NFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSS 433
              ++ G G  VN+WVE GKFFRES LK+G ++ MPDI+DKMP RSFLPR I+  LPFS+
Sbjct: 393 R--SSLGGGKTVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFST 452

Query: 434 SKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNV 493
           SKI E+K +FHA + STME +ITDA+ ECER P+  ETKRCVGSAEDMIDFATSVLGR+V
Sbjct: 453 SKIAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSV 512

Query: 494 VARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADIL 553
           V R T +V+GS E V IG V GINGG +T+SVSCHQSLYPYLLYYCHSVPKVRVYE+D+L
Sbjct: 513 VLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLL 572

Query: 554 SLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 609
             ++K KINHG+AICHMDTS+WG NHGAF+ LGS PG+IEVCHWIFEND++W++AD
Sbjct: 573 DPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of MC06g0129 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 1.8e-190
Identity = 344/601 (57.24%), Postives = 423/601 (70.38%), Query Frame = 0

Query: 8   SATTAQPALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSV 67
           +A    P+ NPFT KASL RYWN H++   PKP F  SKASPL+AVDS   A+ AS  ++
Sbjct: 29  TAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHAL 88

Query: 68  SMASRFESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNY 127
           +  +     CS+A LFCF +           D NFA Y  +NF NYG+ RL GADSFKNY
Sbjct: 89  N--THHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNY 148

Query: 128 SDGLNSADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLV 187
           S G N A  SF+RY  NS+ H +GFTNYA + NVA   F +YA  + GGSGEF NY    
Sbjct: 149 SGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDA 208

Query: 188 NVPNLRFTAYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILT 247
           N PN RFT+Y   +     +FT Y    N+G QSFTSY KNGNG P EFS Y  GSN++ 
Sbjct: 209 NEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVN 268

Query: 248 STFTGYGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFL 307
           + FT YGE+   ANDSF  YG  GN P + F+ YG+GGN  +  F NYR+ +N+G DSF 
Sbjct: 269 TGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFS 328

Query: 308 SYAKNSNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVS 367
           SYAKNSN+ + NF NYG+SFN G+D+F  YG+ ++G   V FK Y   +SF  Y K GV 
Sbjct: 329 SYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGN-VSFKTYGQGQSFKVYTKDGVV 388

Query: 368 FSEYSNFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVN 427
           F+ YSN  ++ G+   VN+WVE GKFFRE+ LK+G +M MPDI+DKMP R+FLPR I+ N
Sbjct: 389 FARYSNNVSSNGK--TVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKN 448

Query: 428 LPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSV 487
           LPFSSS I E+  +F A E S+M  +I+ A++ECER  S  ETKRCVGSAEDMIDFATSV
Sbjct: 449 LPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSV 508

Query: 488 LGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVY 547
           LGR VV R T NV GS + V IG V GINGG++TR+VSCHQSLYPYLLYYCHSVP+VRVY
Sbjct: 509 LGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVY 568

Query: 548 EADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVA 607
           E D+L  ++ +KINHGVAICH+DTS+W P+HGAFLALGSGPG+IEVCHWIFEND++W++ 
Sbjct: 569 ETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNII 624

Query: 608 D 609
           D
Sbjct: 629 D 624

BLAST of MC06g0129 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 6.1e-178
Identity = 330/602 (54.82%), Postives = 410/602 (68.11%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT K  + RYWN HVSN LPKP FL +KASPL+A   A      +  + ++++  +S 
Sbjct: 35  NPFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLNAAQYATYTKLVADQN-ALSTHLQSF 94

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLG-GADSFKNYSDGLNSAD 136
           CSSANL C  D    +      D +F  YGN+NF NYG    G G ++FKNYS+  +++ 
Sbjct: 95  CSSANLMCAPDLLPSLE-KHTGDIHFTTYGNKNFTNYGTNEPGIGVNTFKNYSE--DASV 154

Query: 137 HSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFT 196
           +SF+RYG  S    + F NYA DGNV    F SY+  + GGSG+F NY    NVP+LRFT
Sbjct: 155 NSFRRYGRGSPRDNK-FDNYAPDGNVIDQSFNSYSTNTPGGSGQFTNYAPNTNVPDLRFT 214

Query: 197 AYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGE 256
           AY       +  F  Y  + NSG QSF SYGKNGNG   +F+SY   +N+  STF  YG+
Sbjct: 215 AYSDQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNGADSKFTSYGNETNVAASTFKNYGQ 274

Query: 257 AGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNS 316
                N +F  Y   GNNP +NF++YG GGN   +TF+NYR+ +NVGDD F +Y K++N+
Sbjct: 275 NANGENQNFTSYSTNGNNPQNNFKNYGVGGNGPSETFTNYRDESNVGDDKFSNYVKDANA 334

Query: 317 GRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFT 376
           G ANF NYG+SFN G D F  YG+G      + FK Y V+ +F  Y K   +FS Y N T
Sbjct: 335 GEANFTNYGQSFNEGTDVFITYGKGG-NDPHINFKTYGVNNTFKDYVKDTATFSNYHNKT 394

Query: 377 TAT---------GRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILV 436
           +           G+ +N NRW+E GKFFRE  LK G +M MPDI+DKMP RSFLPR I  
Sbjct: 395 SQDLASLSEVNGGKKVN-NRWIEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRAIAA 454

Query: 437 NLPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATS 496
            LPFS+SKI E+K+IFHA   S + ++I DAL+ECERAPS  ETK+CV SAEDMIDFATS
Sbjct: 455 KLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPSPGETKQCVNSAEDMIDFATS 514

Query: 497 VLGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRV 556
           VLGRNVV R T N +GS   + IG++KGINGG++T+SVSCHQ+LYP LLYYCHSVPKVRV
Sbjct: 515 VLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCHQTLYPSLLYYCHSVPKVRV 574

Query: 557 YEADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSV 609
           YEADIL   +K KINHGVAICH+DTSSWGP HGAF+ALGSGPG+IEVCHWIFEND++W+ 
Sbjct: 575 YEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFIALGSGPGKIEVCHWIFENDMTWAT 629

BLAST of MC06g0129 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.4e-177
Identity = 329/602 (54.65%), Postives = 408/602 (67.77%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT K  L RYW   +SN LPKP FL +KASPL+A   A      +  + ++ ++  + 
Sbjct: 34  NPFTPKGYLIRYWKKQISNDLPKPWFLLNKASPLNAAQYATYTKLVADQN-ALTTQLHTF 93

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLG-GADSFKNYSDGLNSAD 136
           CSSANL C  D    +    + D +FA Y ++NF NYG    G G ++FKNYS+G N   
Sbjct: 94  CSSANLMCAPDLSPSLE-KHSGDIHFATYSDKNFTNYGTNEPGIGVNTFKNYSEGENIPV 153

Query: 137 HSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFT 196
           +SF+RYG  S    + F NYA DGNV    F SY+  + GGSG+F NY    N PNL FT
Sbjct: 154 NSFRRYGRGSPRDNK-FDNYASDGNVIDQSFNSYSTSTAGGSGKFTNYAANANDPNLHFT 213

Query: 197 AYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGE 256
           +Y          FT Y  E N+G Q F SYGKNGNG   EF SY   +N++ STFT YG+
Sbjct: 214 SYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNGNGANGEFVSYGNDTNVIGSTFTNYGQ 273

Query: 257 AGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNS 316
                +  F  YG  GN P ++F +YG GGN   +TF++YR+ +NVGDD+F +Y K++N 
Sbjct: 274 TANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSYRDQSNVGDDTFTTYVKDANG 333

Query: 317 GRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFT 376
           G ANF NYG+SFN G D F  YG+G      + FK Y V+ +F  Y K   +FS Y N T
Sbjct: 334 GEANFTNYGQSFNEGTDVFTTYGKGG-NDPHINFKTYGVNNTFKDYVKDTATFSNYHNKT 393

Query: 377 TAT---------GRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILV 436
           +           G+ +N NRWVE GKFFRE  LK G +M MPDI+DKMP RSFLPR I  
Sbjct: 394 SQVLASLMEVNGGKKVN-NRWVEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRVIAS 453

Query: 437 NLPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATS 496
            LPFS+SKI E+K+IFHA + S +E++I DAL+ECERAPS  ETKRCV SAEDMIDFATS
Sbjct: 454 KLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAGETKRCVNSAEDMIDFATS 513

Query: 497 VLGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRV 556
           VLGRNVV R T +  GS+  + IG+VKGINGG++T+SVSCHQ+LYPYLLYYCHSVPKVRV
Sbjct: 514 VLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQTLYPYLLYYCHSVPKVRV 573

Query: 557 YEADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSV 609
           YEADIL   +K KINHGVAICH+DTSSWGP+HGAF+ALGSGPG+IEVCHWIFEND++W++
Sbjct: 574 YEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGPGKIEVCHWIFENDMTWAI 630

BLAST of MC06g0129 vs. NCBI nr
Match: XP_038878610.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 509/606 (83.99%), Postives = 550/606 (90.76%), Query Frame = 0

Query: 3   FMQVCSATTAQPALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHA 62
           F  V  A+      NPFTAKASL RYWN+HVSNQLP PDFLFSKASPLSAVD+AVLA HA
Sbjct: 17  FFHVSFASADHQQSNPFTAKASLLRYWNSHVSNQLPPPDFLFSKASPLSAVDAAVLAQHA 76

Query: 63  SRGSVSMASRFESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGAD 122
           +RGS+S   R ES C SANLFC FDSET++SV R KDA+F LYGN+NFVNYG  RLGG D
Sbjct: 77  ARGSLSF--RLESFCYSANLFCSFDSETEISVPRLKDADFKLYGNKNFVNYGGARLGGTD 136

Query: 123 SFKNYSDGLNSADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVN 182
           SFKNYSDGLN+ADHSFKRYGGNSSNH EGFTNYAEDGNVA S+FISYA ESKGGSGEFVN
Sbjct: 137 SFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVANSNFISYATESKGGSGEFVN 196

Query: 183 YEKLVNVPNLRFTAYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEG 242
           Y+KLVNVPNLRFTAYDL+SR HKLSFTGYGNETNSGSQ+FTSYGK GNGVPQEFSSYS+G
Sbjct: 197 YDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFTSYGKKGNGVPQEFSSYSDG 256

Query: 243 SNILTSTFTGYGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVG 302
           SNILTSTFTGYGE+GK+ANDSFK YG++GNNPH+NF++YG GGNS+IDTFSNYRNGANVG
Sbjct: 257 SNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVG 316

Query: 303 DDSFLSYAKNSNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYA 362
           DDSF SYAKNSNSGR NFANYG+SFNHGNDSF EYG+GSIGRT VGFK YSVDR+FTTYA
Sbjct: 317 DDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGRTTVGFKSYSVDRNFTTYA 376

Query: 363 KTGVSFSEYSNFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR 422
           + GVSFSEYSNFTTATG GI+VN+ VE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR
Sbjct: 377 QKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR 436

Query: 423 PILVNLPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMID 482
           PILVNLPFSSS+I E KEIFHAR+GSTMERVIT+AL ECERAPS  ETKRCVGSAEDMID
Sbjct: 437 PILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECERAPSPGETKRCVGSAEDMID 496

Query: 483 FATSVLGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVP 542
           FAT+VLGR VVAR TANV+GS E VEIG VKGINGG+LT SVSCHQSLYPYLLYYCHSVP
Sbjct: 497 FATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSSVSCHQSLYPYLLYYCHSVP 556

Query: 543 KVRVYEADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDV 602
           KVR+YEADIL L+TK+KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFEND+
Sbjct: 557 KVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDM 616

Query: 603 SWSVAD 608
           SWSVAD
Sbjct: 617 SWSVAD 620

BLAST of MC06g0129 vs. NCBI nr
Match: XP_022921994.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 501/592 (84.63%), Postives = 541/592 (91.39%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF+KASPLSAVD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + SV R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGFTNYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTFTGYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+GNNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEYG+GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTME VIT ALAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVV R 
Sbjct: 462 ETKQIFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRT 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSKEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of MC06g0129 vs. NCBI nr
Match: KAG6589452.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023135.1 Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 500/592 (84.46%), Postives = 542/592 (91.55%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF+KASPLSAVD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + S+ R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSIPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGF NYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYGGNSSNHKEGFMNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTFTGYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+GNNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEYG+GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTME VIT ALAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVVAR 
Sbjct: 462 ETKQIFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVART 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS+E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of MC06g0129 vs. NCBI nr
Match: XP_022988353.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita maxima])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 502/592 (84.80%), Postives = 541/592 (91.39%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF KASPLSAVD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFVKASPLSAVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + SV R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGFTNYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTFTGYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+ NNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSSNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEY +GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYAKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTMERVIT ALAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVVAR 
Sbjct: 462 ETKQIFHARNGSTMERVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVART 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS+E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFENDVSWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDVSWSVAD 626

BLAST of MC06g0129 vs. NCBI nr
Match: XP_023516985.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 988 bits (2553), Expect = 0.0
Identity = 498/592 (84.12%), Postives = 540/592 (91.22%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF+KASPLS VD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSVVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + SV R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRY GNSSNH+EGFTNYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYSGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTF+GYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFSGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+GNNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEYG+GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTME VIT  LAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVVAR 
Sbjct: 462 ETKQIFHARNGSTMEHVITSTLAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVART 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS+E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPKHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of MC06g0129 vs. ExPASy TrEMBL
Match: A0A6J1E242 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111430079 PE=4 SV=1)

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 501/592 (84.63%), Postives = 541/592 (91.39%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF+KASPLSAVD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFAKASPLSAVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + SV R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGFTNYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTFTGYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+GNNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSGNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEYG+GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYGKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTME VIT ALAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVV R 
Sbjct: 462 ETKQIFHARNGSTMEHVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRT 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSKEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of MC06g0129 vs. ExPASy TrEMBL
Match: A0A6J1JM21 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485620 PE=4 SV=1)

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 502/592 (84.80%), Postives = 541/592 (91.39%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFTAKASL RYWN HVSNQLPKPDFLF KASPLSAVD+AV A HA+ GS++  SR E+ 
Sbjct: 42  NPFTAKASLVRYWNTHVSNQLPKPDFLFVKASPLSAVDAAVFAKHAALGSLT--SRLETF 101

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS+ + SV R KDA+F LYGN+NF NYGA R+GGADSFKNYSDGLN+ADH
Sbjct: 102 CYSANLFCSFDSQ-ETSVPRFKDADFKLYGNKNFNNYGAARIGGADSFKNYSDGLNTADH 161

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGFTNYAEDGNVA ++FISYAAESKGGSGEFVNY+K+VNVPNLRFTA
Sbjct: 162 SFKRYGGNSSNHKEGFTNYAEDGNVANNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTA 221

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDLDSRAHKLSFT YGNETNSGSQSFTSYGKNGNGVPQEFSSYS+GSNILTSTFTGYGE+
Sbjct: 222 YDLDSRAHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGES 281

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GKSANDSFK YGL+ NNPHSNF++YG GG SQIDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 282 GKSANDSFKAYGLSSNNPHSNFKNYGKGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSG 341

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFA+YG+SFNHGNDSFKEY +GSIG+T VGF+ YSVDR+FTTYA+ GVSFSEYS    
Sbjct: 342 RVNFASYGQSFNHGNDSFKEYAKGSIGQTTVGFRSYSVDRNFTTYAQKGVSFSEYS---- 401

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GINVNRWVE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVN+PFSSSKI 
Sbjct: 402 ATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKIS 461

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E K+IFHAR GSTMERVIT ALAECERAPS  ETKRCVGSAEDMIDFAT+VLGRNVVAR 
Sbjct: 462 ETKQIFHARNGSTMERVITSALAECERAPSMGETKRCVGSAEDMIDFATAVLGRNVVART 521

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TA+V+GS+E VEIG VKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL L+T
Sbjct: 522 TASVNGSTEKVEIGEVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKT 581

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
            +KIN GVAICHMDTSSWGP HGAF+ALGSGPG+IEVCHWIFENDVSWSVAD
Sbjct: 582 NEKINRGVAICHMDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDVSWSVAD 626

BLAST of MC06g0129 vs. ExPASy TrEMBL
Match: A0A1S3BVD2 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 PE=4 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 489/607 (80.56%), Postives = 542/607 (89.29%), Query Frame = 0

Query: 2   FFMQVCSATTAQPALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAH 61
           FF Q+ SA ++    NPFT KASL RYW  HVS  LP PDF  SKASPLS+VD+AVLA H
Sbjct: 48  FFPQISSAASS----NPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATH 107

Query: 62  ASRGSVSMASRFESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGA 121
           A+  S+S  SR ES C SANLFC FDS++++SV R KDA+F LYGN+NF NYG  RLGG 
Sbjct: 108 AAGASLS--SRLESFCYSANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGT 167

Query: 122 DSFKNYSDGLNSADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFV 181
           DSFKNYSDGLN+A+HSFKRYGGNSSNH+EGFT+YAEDGNVA S+F+SY AESK GSG F 
Sbjct: 168 DSFKNYSDGLNTAEHSFKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFT 227

Query: 182 NYEKLVNVPNLRFTAYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSE 241
           NY+KLVNVPNLRFTAYDL+SR+HKL+FT YGNETNSGSQSFTSYGKNGNGVPQ+FSSYS+
Sbjct: 228 NYDKLVNVPNLRFTAYDLNSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSD 287

Query: 242 GSNILTSTFTGYGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANV 301
           GSNILTSTFTGYGE+GK+ANDSFKEYG++GNNPH+NF++YG GGNS+IDTFSNYRNGANV
Sbjct: 288 GSNILTSTFTGYGESGKTANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANV 347

Query: 302 GDDSFLSYAKNSNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTY 361
           GDDSF SYAKNSNSGR NFANYG+SFNHGNDSF EYG+GSIG+T VGFK YS+DR+FTTY
Sbjct: 348 GDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTY 407

Query: 362 AKTGVSFSEYSNFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLP 421
           AK GVSFSEYSNFTTATG GI+VN  VE GKFFRESELKQGNVMVMPDIRDKMPPRSFLP
Sbjct: 408 AKNGVSFSEYSNFTTATGSGIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLP 467

Query: 422 RPILVNLPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMI 481
           R ILVNLPFSSS+I E KE+FHAR+GSTME VIT+ALAECERAPSR ETKRCVGSAEDMI
Sbjct: 468 RAILVNLPFSSSRISEAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMI 527

Query: 482 DFATSVLGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSV 541
           DFAT+VLGR VV R TANV+GS E VEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSV
Sbjct: 528 DFATTVLGRRVVVRTTANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSV 587

Query: 542 PKVRVYEADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFEND 601
           PKVR+YEADIL ++TK+KIN GVAICH+DTSSWGP HGAF+ALGS PG+IEVCHWIFEND
Sbjct: 588 PKVRIYEADILRVETKEKINRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFEND 647

Query: 602 VSWSVAD 608
           +SWSVAD
Sbjct: 648 MSWSVAD 648

BLAST of MC06g0129 vs. ExPASy TrEMBL
Match: A0A5D3BIQ0 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001790 PE=4 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 484/592 (81.76%), Postives = 534/592 (90.20%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT KASL RYW  HVS  LP PDF  SKASPLS+VD+AVLA HA+  S+S  SR ES 
Sbjct: 9   NPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLS--SRLESF 68

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           C SANLFC FDS++++SV R KDA+F LYGN+NF NYG  RLGG DSFKNYSDGLN+A+H
Sbjct: 69  CYSANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEH 128

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SFKRYGGNSSNH+EGFT+YAEDGNVA S+F+SY AESK GSG F NY+KLVNVPNLRFTA
Sbjct: 129 SFKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTA 188

Query: 197 YDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGEA 256
           YDL+SR+HKL+FT YGNETNSGSQSFTSYGKNGNGVPQ+FSSYS+GSNILTSTFTGYGE+
Sbjct: 189 YDLNSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGES 248

Query: 257 GKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNSG 316
           GK+ANDSFKEYG++GNNPH+NF++YG GGNS+IDTFSNYRNGANVGDDSF SYAKNSNSG
Sbjct: 249 GKTANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSG 308

Query: 317 RANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFTT 376
           R NFANYG+SFNHGNDSF EYG+GSIG+T VGFK YS+DR+FTTYAK GVSFSEYSNFTT
Sbjct: 309 RVNFANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTT 368

Query: 377 ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSKIY 436
           ATG GI+VN  VE GKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFSSS+I 
Sbjct: 369 ATGSGIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRIS 428

Query: 437 EMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARL 496
           E KE+FHAR+GSTME VIT+ALAECERAPSR ETKRCVGSAEDMIDFAT+VLGR VV R 
Sbjct: 429 EAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRT 488

Query: 497 TANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQT 556
           TANV+GS E VEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSVPKVR+YEADIL ++T
Sbjct: 489 TANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVET 548

Query: 557 KQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 608
           K+KIN GVAICH+DTSSWGP HGAF+ALGS PG+IEVCHWIFEND+SWSVAD
Sbjct: 549 KEKINRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 598

BLAST of MC06g0129 vs. ExPASy TrEMBL
Match: A0A0A0LP63 (Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa_1G005490 PE=4 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 485/607 (79.90%), Postives = 542/607 (89.29%), Query Frame = 0

Query: 2   FFMQVCSATTAQPALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAH 61
           FF Q+ SA++     NPFT KASL RYW  HVS  LP PDF  SKASPLSAVD+A LA H
Sbjct: 46  FFPQISSASS-----NPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSAVDAAALATH 105

Query: 62  ASRGSVSMASRFESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGA 121
           A+  S+S  SR ES C SANLFC FDS+++ SV R+KDA+F LYGN+NFVNYG  RLGG 
Sbjct: 106 AAGASLS--SRLESFCFSANLFCSFDSQSETSVPRSKDADFKLYGNKNFVNYGGSRLGGT 165

Query: 122 DSFKNYSDGLNSADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFV 181
           DSFKNYSDGLN+A+HSFKRYGGNSSNH+EGFT+YAEDGNVA S+F+SY A SK GSGEF 
Sbjct: 166 DSFKNYSDGLNTAEHSFKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAGSKSGSGEFT 225

Query: 182 NYEKLVNVPNLRFTAYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSE 241
           NY+KLVNVPNLRFTAYDL+SR+HKLSFT YGNETNSGSQSFTSYGKNGNGVPQ+FSSYS+
Sbjct: 226 NYDKLVNVPNLRFTAYDLNSRSHKLSFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSD 285

Query: 242 GSNILTSTFTGYGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANV 301
           GSNILTSTFTGYGE+GK+ANDSFKEYG++GNNPH+NF++YG GGNS+IDTFSNYRNGANV
Sbjct: 286 GSNILTSTFTGYGESGKTANDSFKEYGISGNNPHTNFKNYGKGGNSEIDTFSNYRNGANV 345

Query: 302 GDDSFLSYAKNSNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTY 361
           GDDSF SYAKNSNSGR NFANYG+SFNHGNDSF EYG+GS+G+T VGFK YSVDR+FTTY
Sbjct: 346 GDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSMGKTTVGFKSYSVDRNFTTY 405

Query: 362 AKTGVSFSEYSNFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLP 421
           AK GVSFS Y+NFTTATG GI+ N  VE GKFFRESELKQGNVMVMPDI+DKMPPRSFLP
Sbjct: 406 AKNGVSFSVYNNFTTATGSGIHANSHVEPGKFFRESELKQGNVMVMPDIKDKMPPRSFLP 465

Query: 422 RPILVNLPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMI 481
           R ILVNLPFSSS+I E KE+FHAR+GSTME VIT+ALAECERAPSR ETKRCVGSAEDMI
Sbjct: 466 RSILVNLPFSSSRISEAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMI 525

Query: 482 DFATSVLGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSV 541
           DFAT+VLG+ VV R TANV+GS E +EIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSV
Sbjct: 526 DFATTVLGQRVVVRTTANVNGSKEKIEIGRVKGINGGDLTRSVSCHQSLYPYLLYYCHSV 585

Query: 542 PKVRVYEADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFEND 601
           PKVR+YEADIL ++TK+KIN GVAICH+DTSSWGP HGAF+ALGSGPG+IEVCHWIFEND
Sbjct: 586 PKVRIYEADILKMETKEKINRGVAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFEND 645

Query: 602 VSWSVAD 608
           +SW+VAD
Sbjct: 646 MSWNVAD 645

BLAST of MC06g0129 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 690.6 bits (1781), Expect = 1.1e-198
Identity = 356/596 (59.73%), Postives = 436/596 (73.15%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT KASL RYWN  +  Q P+ +FL SKASPL+AVDSA  +  A+    S+ +RF   
Sbjct: 35  NPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAAN--SLPTRFPDF 94

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           CS+ANLFCF D    +      D  F++Y  +NF NYG  R GGADSFKNYS   N    
Sbjct: 95  CSAANLFCFPDLGASLE-KHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTD 154

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SF+RY  N++ H + FT Y E+ NV    F SY     GG+G+F NY+  VN P  RFTA
Sbjct: 155 SFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTA 214

Query: 197 YDLDSRAHKLSFTGYGNETNSGS-QSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGE 256
           Y         +F  Y +E N+G+ QSFTSYGKNGNGVP EF+SY   SN++ S F+ YGE
Sbjct: 215 YSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGE 274

Query: 257 AGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNS 316
           +G +AND+F  YG  GN P +NF +YG  GN+ +DTF+NYR+ ANVGDDSF SYAK+SNS
Sbjct: 275 SGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNS 334

Query: 317 GRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFT 376
            + NF NYG+SFN G+++F  YG+G+ G + + FK Y+ + +F  YAK GV+F++Y+  T
Sbjct: 335 EKVNFVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVST 394

Query: 377 T---ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSS 436
           T     G G  VN+W+E GKFFRES LK+G V+ MPDI+DKMP RSFLPR I+  LPFS+
Sbjct: 395 TTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFST 454

Query: 437 SKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNV 496
           SK+ E+K IFHA E STM  +ITDA+ ECER PS  ETKRCVGSAEDMIDFATSVLGR+V
Sbjct: 455 SKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSV 514

Query: 497 VARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADIL 556
           V R T NV+GS E V IG V GINGG+LT++VSCHQSLYPYLLYYCHSVPKVRVYEAD+L
Sbjct: 515 VLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLL 574

Query: 557 SLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 609
            L +K+KINHG+AICHMDTSSWGP+HGAFLALGS PGRIEVCHWIFEND++W++AD
Sbjct: 575 ELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of MC06g0129 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 690.6 bits (1781), Expect = 1.1e-198
Identity = 356/596 (59.73%), Postives = 436/596 (73.15%), Query Frame = 0

Query: 17  NPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRFESL 76
           NPFT KASL RYWN  +  Q P+ +FL SKASPL+AVDSA  +  A+    S+ +RF   
Sbjct: 35  NPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAAN--SLPTRFPDF 94

Query: 77  CSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNSADH 136
           CS+ANLFCF D    +      D  F++Y  +NF NYG  R GGADSFKNYS   N    
Sbjct: 95  CSAANLFCFPDLGASLE-KHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTD 154

Query: 137 SFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLRFTA 196
           SF+RY  N++ H + FT Y E+ NV    F SY     GG+G+F NY+  VN P  RFTA
Sbjct: 155 SFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTA 214

Query: 197 YDLDSRAHKLSFTGYGNETNSGS-QSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTGYGE 256
           Y         +F  Y +E N+G+ QSFTSYGKNGNGVP EF+SY   SN++ S F+ YGE
Sbjct: 215 YSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGE 274

Query: 257 AGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKNSNS 316
           +G +AND+F  YG  GN P +NF +YG  GN+ +DTF+NYR+ ANVGDDSF SYAK+SNS
Sbjct: 275 SGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNS 334

Query: 317 GRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYSNFT 376
            + NF NYG+SFN G+++F  YG+G+ G + + FK Y+ + +F  YAK GV+F++Y+  T
Sbjct: 335 EKVNFVNYGQSFNPGSETFTGYGKGAEG-SKLSFKTYTPNSTFKDYAKKGVAFAKYNVST 394

Query: 377 T---ATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSS 436
           T     G G  VN+W+E GKFFRES LK+G V+ MPDI+DKMP RSFLPR I+  LPFS+
Sbjct: 395 TTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFST 454

Query: 437 SKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNV 496
           SK+ E+K IFHA E STM  +ITDA+ ECER PS  ETKRCVGSAEDMIDFATSVLGR+V
Sbjct: 455 SKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSV 514

Query: 497 VARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADIL 556
           V R T NV+GS E V IG V GINGG+LT++VSCHQSLYPYLLYYCHSVPKVRVYEAD+L
Sbjct: 515 VLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLL 574

Query: 557 SLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 609
            L +K+KINHG+AICHMDTSSWGP+HGAFLALGS PGRIEVCHWIFEND++W++AD
Sbjct: 575 ELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of MC06g0129 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 670.2 bits (1728), Expect = 1.5e-192
Identity = 346/596 (58.05%), Postives = 427/596 (71.64%), Query Frame = 0

Query: 14  PALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSVSMASRF 73
           P +NPFT KASL RYWN  + +Q P   FL SKASPL+AV SA  A  AS    ++  R 
Sbjct: 33  PEINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLASEN--ALPDRL 92

Query: 74  ESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNYSDGLNS 133
              CS+ANLFCF D    +    + D  F++Y  RNF NYG    GGADSFK YS   N+
Sbjct: 93  PDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYDQRNFTNYGTIHPGGADSFKKYSQNGNA 152

Query: 134 ADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLVNVPNLR 193
              SF+RY  +++ H + FT YA++ NV    F SY   + GGSG+F NY+  VN P  R
Sbjct: 153 VTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSGVNNPTTR 212

Query: 194 FTAYDLDSRAHKLSFTGYGNETNSG-SQSFTSYGKNGNGVPQEFSSYSEGSNILTSTFTG 253
           F AY  +      +F  Y +E NSG  QSFTSYGKNGNG P EFSSY   SN++ S F+ 
Sbjct: 213 FKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNVIGSDFSN 272

Query: 254 YGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFLSYAKN 313
           YGE G +AND+FK YG  GN P +NF+SYG  GN+ +DTF NYR+ ANVGDDSF SYAKN
Sbjct: 273 YGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDSFSSYAKN 332

Query: 314 SNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVSFSEYS 373
           SN  + +F NYG+S N G++SF  YG+G+ G   + FK Y+ + +F  Y KTGV F++Y+
Sbjct: 333 SNFEKVDFVNYGKSINPGSESFSGYGKGAEG-NKIDFKTYTQNSTFKDYTKTGVEFAKYN 392

Query: 374 NFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSS 433
              ++ G G  VN+WVE GKFFRES LK+G ++ MPDI+DKMP RSFLPR I+  LPFS+
Sbjct: 393 R--SSLGGGKTVNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIVSKLPFST 452

Query: 434 SKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNV 493
           SKI E+K +FHA + STME +ITDA+ ECER P+  ETKRCVGSAEDMIDFATSVLGR+V
Sbjct: 453 SKIAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFATSVLGRSV 512

Query: 494 VARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADIL 553
           V R T +V+GS E V IG V GINGG +T+SVSCHQSLYPYLLYYCHSVPKVRVYE+D+L
Sbjct: 513 VLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVRVYESDLL 572

Query: 554 SLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVAD 609
             ++K KINHG+AICHMDTS+WG NHGAF+ LGS PG+IEVCHWIFEND++W++AD
Sbjct: 573 DPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of MC06g0129 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 667.2 bits (1720), Expect = 1.3e-191
Identity = 344/601 (57.24%), Postives = 423/601 (70.38%), Query Frame = 0

Query: 8   SATTAQPALNPFTAKASLARYWNAHVSNQLPKPDFLFSKASPLSAVDSAVLAAHASRGSV 67
           +A    P+ NPFT KASL RYWN H++   PKP F  SKASPL+AVDS   A+ AS  ++
Sbjct: 29  TAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHAL 88

Query: 68  SMASRFESLCSSANLFCFFDSETDVSVSRAKDANFALYGNRNFVNYGAGRLGGADSFKNY 127
           +  +     CS+A LFCF +           D NFA Y  +NF NYG+ RL GADSFKNY
Sbjct: 89  N--THHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNY 148

Query: 128 SDGLNSADHSFKRYGGNSSNHREGFTNYAEDGNVATSDFISYAAESKGGSGEFVNYEKLV 187
           S G N A  SF+RY  NS+ H +GFTNYA + NVA   F +YA  + GGSGEF NY    
Sbjct: 149 SGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDA 208

Query: 188 NVPNLRFTAYDLDSRAHKLSFTGYGNETNSGSQSFTSYGKNGNGVPQEFSSYSEGSNILT 247
           N PN RFT+Y   +     +FT Y    N+G QSFTSY KNGNG P EFS Y  GSN++ 
Sbjct: 209 NEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVN 268

Query: 248 STFTGYGEAGKSANDSFKEYGLTGNNPHSNFRSYGNGGNSQIDTFSNYRNGANVGDDSFL 307
           + FT YGE+   ANDSF  YG  GN P + F+ YG+GGN  +  F NYR+ +N+G DSF 
Sbjct: 269 TGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFS 328

Query: 308 SYAKNSNSGRANFANYGRSFNHGNDSFKEYGRGSIGRTAVGFKGYSVDRSFTTYAKTGVS 367
           SYAKNSN+ + NF NYG+SFN G+D+F  YG+ ++G   V FK Y   +SF  Y K GV 
Sbjct: 329 SYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGN-VSFKTYGQGQSFKVYTKDGVV 388

Query: 368 FSEYSNFTTATGRGINVNRWVEAGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVN 427
           F+ YSN  ++ G+   VN+WVE GKFFRE+ LK+G +M MPDI+DKMP R+FLPR I+ N
Sbjct: 389 FARYSNNVSSNGK--TVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKN 448

Query: 428 LPFSSSKIYEMKEIFHAREGSTMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSV 487
           LPFSSS I E+  +F A E S+M  +I+ A++ECER  S  ETKRCVGSAEDMIDFATSV
Sbjct: 449 LPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSV 508

Query: 488 LGRNVVARLTANVSGSSEMVEIGAVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVY 547
           LGR VV R T NV GS + V IG V GINGG++TR+VSCHQSLYPYLLYYCHSVP+VRVY
Sbjct: 509 LGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVY 568

Query: 548 EADILSLQTKQKINHGVAICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSWSVA 607
           E D+L  ++ +KINHGVAICH+DTS+W P+HGAFLALGSGPG+IEVCHWIFEND++W++ 
Sbjct: 569 ETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNII 624

Query: 608 D 609
           D
Sbjct: 629 D 624

BLAST of MC06g0129 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 114.0 bits (284), Expect = 4.2e-25
Identity = 71/219 (32.42%), Postives = 104/219 (47.49%), Query Frame = 0

Query: 390 AGKFFRESELKQGNVM-VMPDIRDKMPPR-SFLPRPILVNLPFSSSKIYEMKEIFHAREG 449
           A  FF E +L +G  M V  +  D    + +FLPR     +PF S K  E  + F    G
Sbjct: 173 AALFFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAG 232

Query: 450 STMERVITDALAECERAPSRDETKRCVGSAEDMIDFATSVLGRNVVARLTANVSGSSEMV 509
           S    ++   + ECE      E K C  S E M+DF+ S LG+  V  ++  V+  +  +
Sbjct: 233 SEEAEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPM 292

Query: 510 EIG--AVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRVYEADILSLQTKQKINHGVA 569
           +    A  G+      +SV CH+  YP+ ++YCH      VY   +      +     VA
Sbjct: 293 QKYKIAAAGVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVYAVPLEGENGMRA--KAVA 352

Query: 570 ICHMDTSSWGPNHGAFLALGSGPGRIEVCHWIFENDVSW 605
           +CH +TS+W PNH AF  L   PG + VCH++ E  V W
Sbjct: 353 VCHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389