MC06g0126 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0126
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCASP-like protein
LocationMC06: 1033257 .. 1036543 (-)
RNA-Seq ExpressionMC06g0126
SyntenyMC06g0126
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGGACCCTAATTTCGTGGAATAAACGGCGTCGTAAAGAGGTAGAGGAAACAGCGCGCCAGATTCAATTTGACAAAAGTTTAGGGGTTTAAGATTAATAAAAAAAATAAATTGACACGGAATCGCAGCTCACTTTACTTTGTCGGAGAGACTCTAAAATACGGTAGCATCCACTATTCACAACCTTTTTCCCCAAATTACATTCCGCCACGTCAGATGAAGAAACCTTACGTGTAATCAGAGTCCTTCTGCTTATTCCAATCCCATACTCTGTGCCGCACTGGAATATATTCTCCACCGTAACTTTTGTAACCATTAATCAGATGGCGCCACGTGGCCCCCACCCGATTAAGCCCCAAAAATACGAAACGCGAACTTAGAAACGGGTCTCAGTAAAGTCCTTTACTCTTTCTTTTTCCCGACTTCCCAAACAGCCTTTTTTTTCTTCCTTAAATTTCAATTCTTCTTCTAAATCTTTCAATAAAATCCGTTGAAACTTGGAACGAAATGATTGAGACGAAATTGAAAACTCAGAGATCGAACGACTCGTCGTCTATGAGGAAGTCGTCGTCGAGAAATTCCGAGTCGTCGCTTCACAGTCATATGGAGTCTCCTCACTCGCCACTGCGGTTCCACTCGCCGCTCCGATCCGACGCCGGCGACCCTCCCGAGACGCCTCCTTATGCCTCTCCGGATACCTCCCCTGAGAAGCTGCCTGATAACTCTAAGGCGATTGTTGTGGTTGATGTTGTTGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCGACTCCAGGAAGCCGCCCGCGGAGAACGCCAACTTCCCGGCGGATAGGTCGACTTCGTCTAAGGTTATGTTTAATCGGGCAATGAGGGAGGAGGTGCCGCAGTCGGTGACGAAGGTGGGGCCGGCTGCGACTGGAGGAGTGGAGGGTGGTGGTGGTGGCGGCGGCGGGAGATCGCCGAAGCAAATGGCGCCGGCACCGAGGAGGTCGAAGGCTGATGCGTTAACGAAGGCGGCTTTAGGCTTTAGAGTGTGTGAAGTGGCAGTTTGTTTGATTTCTTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGCGGTGACTCCTTTGATCGTTACAAGGAATACAGGTTTTTTTCCTTTTGTTTTTACACAATTTTCTTGACGCCTAATGACTATGGCTTTTTTATCTTAAGGCTATTAAACTAGAGAATATTGCAAGTTGGAGTTGGTGTTGCTTCTGGCTGTTGTGGAATTTGTACATTTAATTTTGATAATTTGCTTCTGAATTGACAAAAAGTGTCGTTTGTTCAATTCCTTCGGTTTAATCTGTGTGGATGTAACTATTTTAGCTTGTTCAGTTTGCGTCAACTAAATTTGCATTGTTAATCTTAAACTTGTAGCATGATCAGTGGGACCTCATAGGGATTTTACCGGACCTATTAGTTTTCCATTATTTGGATACTATTAATTTCCTTCCAAGGAGTGTCAGGGCTTGAAATAGTCACTGTTATTAATGGTTTTGAGAGTCTTCTGTTTCTTGTGTGTACCTCATTGTCATCACATGAGTAGGTGCTTTAATTGTAGCTTCGATTTCAAGGTACGTCTTGATCATTCTAAATGACGAGTGCCATGTTAAAAAATGGAGGTGTCACCTTCGTTCCAACCCATTGATGGTAAAATTCTCAATGATCTCAACAATCCAAAAGAATTCTTTAGCTACACTATTCATTTCTGTCTGATCCACAACTTTTTGGTACATATTCTGACATGGAAACCTTTATTCCTTCCCAAGCCATTTGCTGCTTTGAGTTACAAGTTTTTGGTCCTCATGTTGATTTTTGGCATGATTTGAAGTGTGTGAACTTTGAAGGATTGTTGTGGAAGAAATTTCACTATCAGTGTTGTTGCATAATTTCCATGCCTTTTTGTGATCAGTCATGCATGACATTATTCTTAGCTTCCCTTGTATTGCAAGAATACATCAATTATCCGTGCTATAATGTGAGAACCGTACCTATATAGAATCTCATTTTAAATTATCTCTTCCTTTTGCACTTTTCTACTAGTTTATATGTTTTTCCCTGCCTTATATTATCTAATTAAGTCCATTTGTTAACAGGTACTGCCTCACTGTGAATGTAATTGCATTTATATATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAAAAACATATGATACGTCATTATCTCCGCTATCACTTTGATTTCTTCGTGGATCAGGTTAGTTGGGCAAATATTGGCTCTGGATGAATATTGTTTAATAATATCTATAGCCATGTTTTCACTGCTCCAATGAATTAATGTGTTTCTACGGTTGTGGTTTGTTTAGTACAGTACTATAACCTGATGAATAGCAATTAGCATACTTGGGCTAGAAGAAACTCGCTGAATTTGTAATGTTGCTTGGAAGAACACATGCACTTAGAGTACTTTTTATGCTGTATAGATTGACTGAGGATCACTGAACTGAAATTAGAATAATCATTCGAGTGAAGATGAACTTGATTGCTGATCTCTTGTCTTCTCTAATGATTGATTGTGCGGAAGTGGATGTGGATGGTTCAAACTTCTGTCTAATTCAAATATCTAAATTACTAAATTACTGGGAGTAAATTATTGTAGCTGATCTGGTCATCAAGTCTTCTCCCTTCTTGAGTCGTAAATTGTAATTTCTTGATATCCGAGATTTGTTAGCTACTCTGTGGCTTAGGTTCTTCATCAACCGATGATGTAATCCTTGTTCTGTTGACTATATACTGGCAGGTACTAGCATATCTTCTCATATCATCGTCATCTTCGGCCGCCACCCGTGTGGACGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACGCAGATGGCCACTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCCTTTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCCTGCGTGAAATTAAAATCTCAGCCATGTTCGTTTTTCCCTGCAAAGAGAAAATTTTATATTGTCAATCCAAAATTATTATGATTTTTTTTTTCTGATGTTGTACAGAGATATCATAGCTAGCTTTTGACCTTACAATAAAGAGGATACCAAATTGTAAAACGGTTGGGTTATTTTTATTGGATATTTTTTTTTGCATATAAATGAAGCTTCTATGCTCTTAAGCAGCAAATGGCCCCTATATAGAATGATAGAACATGAACATGGTTCATCATAAGCATTTATTTTC

mRNA sequence

GCAGGACCCTAATTTCGTGGAATAAACGGCGTCGTAAAGAGGTAGAGGAAACAGCGCGCCAGATTCAATTTGACAAAAGTTTAGGGGTTTAAGATTAATAAAAAAAATAAATTGACACGGAATCGCAGCTCACTTTACTTTGTCGGAGAGACTCTAAAATACGGTAGCATCCACTATTCACAACCTTTTTCCCCAAATTACATTCCGCCACGTCAGATGAAGAAACCTTACGTGTAATCAGAGTCCTTCTGCTTATTCCAATCCCATACTCTGTGCCGCACTGGAATATATTCTCCACCGTAACTTTTGTAACCATTAATCAGATGGCGCCACGTGGCCCCCACCCGATTAAGCCCCAAAAATACGAAACGCGAACTTAGAAACGGGTCTCAGTAAAGTCCTTTACTCTTTCTTTTTCCCGACTTCCCAAACAGCCTTTTTTTTCTTCCTTAAATTTCAATTCTTCTTCTAAATCTTTCAATAAAATCCGTTGAAACTTGGAACGAAATGATTGAGACGAAATTGAAAACTCAGAGATCGAACGACTCGTCGTCTATGAGGAAGTCGTCGTCGAGAAATTCCGAGTCGTCGCTTCACAGTCATATGGAGTCTCCTCACTCGCCACTGCGGTTCCACTCGCCGCTCCGATCCGACGCCGGCGACCCTCCCGAGACGCCTCCTTATGCCTCTCCGGATACCTCCCCTGAGAAGCTGCCTGATAACTCTAAGGCGATTGTTGTGGTTGATGTTGTTGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCGACTCCAGGAAGCCGCCCGCGGAGAACGCCAACTTCCCGGCGGATAGGTCGACTTCGTCTAAGGTTATGTTTAATCGGGCAATGAGGGAGGAGGTGCCGCAGTCGGTGACGAAGGTGGGGCCGGCTGCGACTGGAGGAGTGGAGGGTGGTGGTGGTGGCGGCGGCGGGAGATCGCCGAAGCAAATGGCGCCGGCACCGAGGAGGTCGAAGGCTGATGCGTTAACGAAGGCGGCTTTAGGCTTTAGAGTGTGTGAAGTGGCAGTTTGTTTGATTTCTTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGCGGTGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACTGTGAATGTAATTGCATTTATATATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAAAAACATATGATACGTCATTATCTCCGCTATCACTTTGATTTCTTCGTGGATCAGGTACTAGCATATCTTCTCATATCATCGTCATCTTCGGCCGCCACCCGTGTGGACGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACGCAGATGGCCACTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCCTTTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCCTGCGTGAAATTAAAATCTCAGCCATGTTCGTTTTTCCCTGCAAAGAGAAAATTTTATATTGTCAATCCAAAATTATTATGATTTTTTTTTTCTGATGTTGTACAGAGATATCATAGCTAGCTTTTGACCTTACAATAAAGAGGATACCAAATTGTAAAACGGTTGGGTTATTTTTATTGGATATTTTTTTTTGCATATAAATGAAGCTTCTATGCTCTTAAGCAGCAAATGGCCCCTATATAGAATGATAGAACATGAACATGGTTCATCATAAGCATTTATTTTC

Coding sequence (CDS)

ATGATTGAGACGAAATTGAAAACTCAGAGATCGAACGACTCGTCGTCTATGAGGAAGTCGTCGTCGAGAAATTCCGAGTCGTCGCTTCACAGTCATATGGAGTCTCCTCACTCGCCACTGCGGTTCCACTCGCCGCTCCGATCCGACGCCGGCGACCCTCCCGAGACGCCTCCTTATGCCTCTCCGGATACCTCCCCTGAGAAGCTGCCTGATAACTCTAAGGCGATTGTTGTGGTTGATGTTGTTGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCGACTCCAGGAAGCCGCCCGCGGAGAACGCCAACTTCCCGGCGGATAGGTCGACTTCGTCTAAGGTTATGTTTAATCGGGCAATGAGGGAGGAGGTGCCGCAGTCGGTGACGAAGGTGGGGCCGGCTGCGACTGGAGGAGTGGAGGGTGGTGGTGGTGGCGGCGGCGGGAGATCGCCGAAGCAAATGGCGCCGGCACCGAGGAGGTCGAAGGCTGATGCGTTAACGAAGGCGGCTTTAGGCTTTAGAGTGTGTGAAGTGGCAGTTTGTTTGATTTCTTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGCGGTGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACTGTGAATGTAATTGCATTTATATATGCGGCATTTCAAGCGTTTGATCTTGTCTATACTCTAATCAAGAAAAAACATATGATACGTCATTATCTCCGCTATCACTTTGATTTCTTCGTGGATCAGGTACTAGCATATCTTCTCATATCATCGTCATCTTCGGCCGCCACCCGTGTGGACGACTGGCAATCGAATTGGGGAAAAGACGAGTTCACGCAGATGGCCACTGCATCGGTCAGCATGTCCTTCCTTGCCTTTGTTGCTTTTGCCTTTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCCTGCGTGA

Protein sequence

MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMFNRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Homology
BLAST of MC06g0126 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 164/310 (52.90%), Postives = 206/310 (66.45%), Query Frame = 0

Query: 17  MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYASPDTSPEKLPDNSKAI 76
           M++++S NSE+   S+ ESPHSPLRFHSPL SDAGD PE+  Y SP+ SP K+ +N K+I
Sbjct: 3   MKRTASSNSEA--QSYNESPHSPLRFHSPL-SDAGDLPES-RYVSPEGSPFKI-ENPKSI 62

Query: 77  VVVDVVDKSTQFSPLPSPY--ADSRKPP---AENANFP----ADRSTSSKVMFNRAMREE 136
           V     +K TQFSPLPSP      + PP     NA  P    +D+S SS V+ N  +RE+
Sbjct: 63  V---AGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVRED 122

Query: 137 VPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVCEVAVCLI 196
             Q+ T+   A   G E                  R    D ++  ALGFR+ EV +C+I
Sbjct: 123 GGQTTTRKAGAPINGEE-----------STRTTVNRARGDDLVSLTALGFRITEVILCVI 182

Query: 197 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKKHMIRHYL 256
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VNVIAF+Y+AF+A D    + K+ +MI    
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGF 242

Query: 257 RYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLAFVAFAFS 316
              F F +DQ+LAYLL+S+SS AATRVDDW SNWGKDEFTQMATAS+++SFLAF AFA S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

Query: 317 SLISGYNLCT 318
           +LIS Y L T
Sbjct: 303 ALISSYRLFT 293

BLAST of MC06g0126 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.4e-66
Identity = 163/310 (52.58%), Postives = 206/310 (66.45%), Query Frame = 0

Query: 17  MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYASPDTSPEKLPDNSKAI 76
           M+++ S NSE+  +S+ ESPHSPLRFHSPL SDAGD PE+  Y SP+ SP K+ +N K+I
Sbjct: 3   MKRTVSSNSEA--YSYNESPHSPLRFHSPL-SDAGDLPES-RYVSPEGSPFKI-ENPKSI 62

Query: 77  VVVDVVDKSTQFSPLPSPY--ADSRKPP---AENANFP----ADRSTSSKVMFNRAMREE 136
           V     +K TQFSPLPSP      + PP     NA  P     D+S SS V+ N  +RE+
Sbjct: 63  V---AGNKLTQFSPLPSPIPPPPPQIPPPRRQRNARVPMNSSLDKSPSSMVVQNSWVRED 122

Query: 137 VPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVCEVAVCLI 196
             Q+ T+   A   G E                  R  + D ++  ALGFR+ EV +C+I
Sbjct: 123 GQQNTTRKAGAPMNGEESA-----------TTAVNRARRDDLVSVTALGFRITEVILCVI 182

Query: 197 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKKHMIRHYL 256
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VNVIAF+Y+AF+A D    + K+ +M+    
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGF 242

Query: 257 RYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLAFVAFAFS 316
              F F +DQ+LAYLL+S+SS AATRVDDW SNWGKDEFTQMATAS+++SFLAF AFA S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

Query: 317 SLISGYNLCT 318
           +LIS Y L T
Sbjct: 303 ALISSYRLFT 293

BLAST of MC06g0126 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-60
Identity = 144/277 (51.99%), Postives = 183/277 (66.06%), Query Frame = 0

Query: 53  PPETPPYASPDT-SPEKLP---DNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANF 112
           P +T P  SP + S EK P   D S AIV  D  + +T FS  PSP   S    +E+   
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVAFD--NSTTHFSSSPSP-PHSLDHSSESEKE 63

Query: 113 PADRSTSSKVMFNRAMREEVP----------QSVTKVGPAATGGVEGGGGGGGGRSPKQM 172
            A     S+   N    EE P          +SV +V P       G G   G RS    
Sbjct: 64  DAKSKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARVGSGRSSGQRSGAVS 123

Query: 173 APAPRRSKADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN 232
           A   R  + + +  +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN
Sbjct: 124 AILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVN 183

Query: 233 VIAFIYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQ 292
           V+AF+Y++FQA DL Y L+K+KH+I H+LR  F+F +DQVLAYLL+S+S++A TRVDDW 
Sbjct: 184 VVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWV 243

Query: 293 SNWGKDEFTQMATASVSMSFLAFVAFAFSSLISGYNL 316
           SNWGKDEFT+MA+AS++MSFLAF+AFAFSSLISGYNL
Sbjct: 244 SNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of MC06g0126 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 138/277 (49.82%), Postives = 178/277 (64.26%), Query Frame = 0

Query: 53  PPETPPYASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADR 112
           P  +P   S + SP    D S AIV  D  + +T  S  PSP   S    +++      R
Sbjct: 2   PSMSPSSISTEKSPPP-SDTSMAIVAFD--NSTTHLSSSPSP-PHSLDHSSDSEKEDEKR 61

Query: 113 STSSKVMFNRAMREEVP----------QSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAP 172
              S+   N    EE P          +SV +V P       G G   G RS   +A   
Sbjct: 62  RPESRRNKNPVKIEETPSPIVVVHNHNRSVKEVVPTRKTARVGSGRSSGQRSGAVLAILR 121

Query: 173 RRSKADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAF 232
           R  + + +   ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VNV+AF
Sbjct: 122 RSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVAF 181

Query: 233 IYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWG 292
           IYA+FQA DL Y L+K+KH+I H+LR  F+F +DQVLAYLL+ +S++A TRVDDW SNWG
Sbjct: 182 IYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMCASTAAVTRVDDWVSNWG 241

Query: 293 KDEFTQMATASVSMSFLAFVAFAFSSLISGYNLCTRD 320
           KD+FT+MA+AS++MSFL F+AFAFSSLISGYNL  +D
Sbjct: 242 KDDFTEMASASIAMSFLTFLAFAFSSLISGYNLFNQD 274

BLAST of MC06g0126 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-42
Identity = 89/156 (57.05%), Postives = 112/156 (71.79%), Query Frame = 0

Query: 162 PRRSK-ADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVI 221
           PR SK A  + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  NVI
Sbjct: 110 PRESKWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVI 169

Query: 222 AFIYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSN 281
            F+Y+ F   DLVY L       RH LR+  +F +DQ+LAYLL S+S+SA+ RVDDWQSN
Sbjct: 170 GFVYSGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSN 229

Query: 282 WGKDEFTQMATASVSMSFLAFVAFAFSSLISGYNLC 317
           WG D+F  +A ASV++S+++FVAFAF SL SGY LC
Sbjct: 230 WGADKFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of MC06g0126 vs. NCBI nr
Match: XP_022135555.1 (CASP-like protein 4A2 [Momordica charantia])

HSP 1 Score: 615 bits (1585), Expect = 1.78e-221
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60
           MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA
Sbjct: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120
           SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180
           NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC
Sbjct: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180

Query: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240
           EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240

Query: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300
           HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA
Sbjct: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300

Query: 301 FVAFAFSSLISGYNLCTRDPA 321
           FVAFAFSSLISGYNLCTRDPA
Sbjct: 301 FVAFAFSSLISGYNLCTRDPA 321

BLAST of MC06g0126 vs. NCBI nr
Match: XP_038878399.1 (CASP-like protein 4A2 [Benincasa hispida])

HSP 1 Score: 550 bits (1417), Expect = 7.65e-196
Identity = 294/324 (90.74%), Postives = 304/324 (93.83%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60
           MIETKLK QRSNDSSSM+KSSSRNSESS+HSHMESPHSPLRFHSPLRSDAGDPPETPPYA
Sbjct: 1   MIETKLKAQRSNDSSSMKKSSSRNSESSVHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120
           SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SR PP EN NF  DRSTSSKVMF
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPP-ENVNFTGDRSTSSKVMF 120

Query: 121 NRAMREEVPQSVTKVGPAATGGVE--GGGGGGGGRSP-KQMAPAPRRSKADALTKAALGF 180
           NRAM+EEVPQSVTKVGPA  GG E  GGGGGGGGRSP KQMAP  RRSK DALTKAALGF
Sbjct: 121 NRAMKEEVPQSVTKVGPAGGGGEENGGGGGGGGGRSPVKQMAPMSRRSKDDALTKAALGF 180

Query: 181 RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLI 240
           RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAFDLV+TLI
Sbjct: 181 RVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLI 240

Query: 241 KKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMS 300
           KK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+A+ASVSMS
Sbjct: 241 KKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMS 300

Query: 301 FLAFVAFAFSSLISGYNLCTRDPA 321
           FLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 FLAFVAFAFSSLISGYNLCTRDPA 323

BLAST of MC06g0126 vs. NCBI nr
Match: XP_008453094.1 (PREDICTED: CASP-like protein 4A2 [Cucumis melo] >KAA0057869.1 CASP-like protein 4A2 [Cucumis melo var. makuwa] >TYJ98559.1 CASP-like protein 4A2 [Cucumis melo var. makuwa])

HSP 1 Score: 537 bits (1384), Expect = 1.05e-190
Identity = 291/331 (87.92%), Postives = 303/331 (91.54%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSS-MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPY 60
           MIETKL+ QRSNDSSS M+KSSSRNS+SS+HSHMESPHSPLRFHSPLRSDAGDP ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVM 120
           ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SRKP  EN NF  DRSTSSKVM
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKP-LENVNFVGDRSTSSKVM 120

Query: 121 FNRAMREEVPQSVTKVGPAATGGVE--------GGGGGGGGRSP-KQMAPAPRRSKADAL 180
           FNRAM+EEVPQSVTKVGPA  GGVE        GGGGGGGGRSP KQMAP  RRSK DAL
Sbjct: 121 FNRAMKEEVPQSVTKVGPAGVGGVENGGGDGGGGGGGGGGGRSPVKQMAPMSRRSKDDAL 180

Query: 181 TKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAF 240
            KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAF
Sbjct: 181 MKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAF 240

Query: 241 DLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMA 300
           DLV+TLIKK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+A
Sbjct: 241 DLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLA 300

Query: 301 TASVSMSFLAFVAFAFSSLISGYNLCTRDPA 321
           +ASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 SASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of MC06g0126 vs. NCBI nr
Match: XP_031746040.1 (CASP-like protein 4A2 [Cucumis sativus])

HSP 1 Score: 527 bits (1357), Expect = 1.52e-186
Identity = 286/334 (85.63%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSS-MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPY 60
           MIETKL+ QRSNDSSS M+KSSSRNS+SS+HSHMESPHSPLRFHSPLRSDAGDP ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVM 120
           ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SRKP  EN NF  DRSTSSK+M
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKP-LENVNFVGDRSTSSKIM 120

Query: 121 FNRAMREEVPQSVTKVGPAATGGVEGGGGGGGG-----------RSP-KQMAPAPRRSKA 180
           FNRAM+EEVPQSVTKVGP   GG E GGGGGGG           RSP KQMAP  RRSK 
Sbjct: 121 FNRAMKEEVPQSVTKVGPVGVGGEENGGGGGGGGGAGAGGGGGGRSPVKQMAPMSRRSKD 180

Query: 181 DALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAF 240
           DAL KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAF
Sbjct: 181 DALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAF 240

Query: 241 QAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFT 300
           QAFDLV+TLIKK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFT
Sbjct: 241 QAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFT 300

Query: 301 QMATASVSMSFLAFVAFAFSSLISGYNLCTRDPA 321
           Q+A+ASVSMSFLAFVAFA SSLISGYNLCTRDPA
Sbjct: 301 QLASASVSMSFLAFVAFAVSSLISGYNLCTRDPA 333

BLAST of MC06g0126 vs. NCBI nr
Match: XP_023530616.1 (CASP-like protein 4A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521 bits (1342), Expect = 1.75e-184
Identity = 281/321 (87.54%), Postives = 296/321 (92.21%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60
           MIE KLK +RS DSSSM+KSSSRNSESS+HSHMESPHSPLRFHSPLRSD GDPPET PYA
Sbjct: 1   MIEMKLKAERSYDSSSMKKSSSRNSESSIHSHMESPHSPLRFHSPLRSDVGDPPETSPYA 60

Query: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120
           SP  SPEKLPDN K+IVVVDV +KSTQFSPL SP A SRK P E ANFP DRSTSSKVMF
Sbjct: 61  SPYASPEKLPDNFKSIVVVDVDNKSTQFSPLRSPNAGSRKLP-EYANFPGDRSTSSKVMF 120

Query: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180
           NRAM+EEVPQSVTKVGPAA GGVE  GGGGGGRSPKQM P PRRSK DALTKAALGFRVC
Sbjct: 121 NRAMKEEVPQSVTKVGPAA-GGVEEDGGGGGGRSPKQMPPPPRRSKDDALTKAALGFRVC 180

Query: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240
           EVAVCLISFSVMASDKT+GWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAFDLVYTLIKK+
Sbjct: 181 EVAVCLISFSVMASDKTKGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVYTLIKKQ 240

Query: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300
           HMIRH +R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMA+ASVSMSFLA
Sbjct: 241 HMIRHRVRSYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLA 300

Query: 301 FVAFAFSSLISGYNLCTRDPA 321
           FVAFA SSLISGYNLC+RDPA
Sbjct: 301 FVAFAVSSLISGYNLCSRDPA 319

BLAST of MC06g0126 vs. ExPASy TrEMBL
Match: A0A6J1C1D1 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1)

HSP 1 Score: 615 bits (1585), Expect = 8.64e-222
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60
           MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA
Sbjct: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120
           SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180
           NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC
Sbjct: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180

Query: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240
           EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240

Query: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300
           HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA
Sbjct: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300

Query: 301 FVAFAFSSLISGYNLCTRDPA 321
           FVAFAFSSLISGYNLCTRDPA
Sbjct: 301 FVAFAFSSLISGYNLCTRDPA 321

BLAST of MC06g0126 vs. ExPASy TrEMBL
Match: A0A5D3BHR8 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001760 PE=3 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 5.09e-191
Identity = 291/331 (87.92%), Postives = 303/331 (91.54%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSS-MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPY 60
           MIETKL+ QRSNDSSS M+KSSSRNS+SS+HSHMESPHSPLRFHSPLRSDAGDP ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVM 120
           ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SRKP  EN NF  DRSTSSKVM
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKP-LENVNFVGDRSTSSKVM 120

Query: 121 FNRAMREEVPQSVTKVGPAATGGVE--------GGGGGGGGRSP-KQMAPAPRRSKADAL 180
           FNRAM+EEVPQSVTKVGPA  GGVE        GGGGGGGGRSP KQMAP  RRSK DAL
Sbjct: 121 FNRAMKEEVPQSVTKVGPAGVGGVENGGGDGGGGGGGGGGGRSPVKQMAPMSRRSKDDAL 180

Query: 181 TKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAF 240
            KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAF
Sbjct: 181 MKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAF 240

Query: 241 DLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMA 300
           DLV+TLIKK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+A
Sbjct: 241 DLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLA 300

Query: 301 TASVSMSFLAFVAFAFSSLISGYNLCTRDPA 321
           +ASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 SASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of MC06g0126 vs. ExPASy TrEMBL
Match: A0A1S3BWI9 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103493912 PE=3 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 5.09e-191
Identity = 291/331 (87.92%), Postives = 303/331 (91.54%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSS-MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPY 60
           MIETKL+ QRSNDSSS M+KSSSRNS+SS+HSHMESPHSPLRFHSPLRSDAGDP ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVM 120
           ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SRKP  EN NF  DRSTSSKVM
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKP-LENVNFVGDRSTSSKVM 120

Query: 121 FNRAMREEVPQSVTKVGPAATGGVE--------GGGGGGGGRSP-KQMAPAPRRSKADAL 180
           FNRAM+EEVPQSVTKVGPA  GGVE        GGGGGGGGRSP KQMAP  RRSK DAL
Sbjct: 121 FNRAMKEEVPQSVTKVGPAGVGGVENGGGDGGGGGGGGGGGRSPVKQMAPMSRRSKDDAL 180

Query: 181 TKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAF 240
            KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAFQAF
Sbjct: 181 MKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAF 240

Query: 241 DLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMA 300
           DLV+TLIKK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ+A
Sbjct: 241 DLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLA 300

Query: 301 TASVSMSFLAFVAFAFSSLISGYNLCTRDPA 321
           +ASVSMSFLAFVAFAFSSLISGYNLCTRDPA
Sbjct: 301 SASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of MC06g0126 vs. ExPASy TrEMBL
Match: A0A0A0LNX3 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1)

HSP 1 Score: 527 bits (1357), Expect = 7.35e-187
Identity = 286/334 (85.63%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSS-MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPY 60
           MIETKL+ QRSNDSSS M+KSSSRNS+SS+HSHMESPHSPLRFHSPLRSDAGDP ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVM 120
           ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYA SRKP  EN NF  DRSTSSK+M
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKP-LENVNFVGDRSTSSKIM 120

Query: 121 FNRAMREEVPQSVTKVGPAATGGVEGGGGGGGG-----------RSP-KQMAPAPRRSKA 180
           FNRAM+EEVPQSVTKVGP   GG E GGGGGGG           RSP KQMAP  RRSK 
Sbjct: 121 FNRAMKEEVPQSVTKVGPVGVGGEENGGGGGGGGGAGAGGGGGGRSPVKQMAPMSRRSKD 180

Query: 181 DALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAF 240
           DAL KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YAAF
Sbjct: 181 DALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAF 240

Query: 241 QAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFT 300
           QAFDLV+TLIKK HMIRH  R +FDFF+DQVLAYLLISSSSSAATRVDDWQSNWGKDEFT
Sbjct: 241 QAFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFT 300

Query: 301 QMATASVSMSFLAFVAFAFSSLISGYNLCTRDPA 321
           Q+A+ASVSMSFLAFVAFA SSLISGYNLCTRDPA
Sbjct: 301 QLASASVSMSFLAFVAFAVSSLISGYNLCTRDPA 333

BLAST of MC06g0126 vs. ExPASy TrEMBL
Match: A0A6J1E1W9 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1)

HSP 1 Score: 518 bits (1333), Expect = 1.72e-183
Identity = 281/321 (87.54%), Postives = 291/321 (90.65%), Query Frame = 0

Query: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60
           MIE KLK   S DSSSMRKSSSRNSE S+HS MESPHSPLRFHSPLRSD GD PETPPYA
Sbjct: 1   MIEAKLK---SIDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRSDVGDLPETPPYA 60

Query: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120
           SPDTSPEK PDNSKAIV+VDVVDKSTQFSPLPSPYA SRK P EN +FP DRSTSSKVMF
Sbjct: 61  SPDTSPEKQPDNSKAIVLVDVVDKSTQFSPLPSPYASSRKTP-ENVSFPGDRSTSSKVMF 120

Query: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180
           NRAM+E+VPQSVTKVGPAA GGVE  GGGGG RSPKQM P  RRSK DALTKAALGFRVC
Sbjct: 121 NRAMKEDVPQSVTKVGPAA-GGVEERGGGGG-RSPKQMPPMSRRSKDDALTKAALGFRVC 180

Query: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240
           EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YA FQAFDLVYTL KK 
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKN 240

Query: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300
           HMIRH+ RYHFDFF+DQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQMA+ASVSMSFLA
Sbjct: 241 HMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLA 300

Query: 301 FVAFAFSSLISGYNLCTRDPA 321
           FVAFA SSLISGYNLCTRD A
Sbjct: 301 FVAFAISSLISGYNLCTRDHA 315

BLAST of MC06g0126 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 254.6 bits (649), Expect = 1.1e-67
Identity = 164/310 (52.90%), Postives = 206/310 (66.45%), Query Frame = 0

Query: 17  MRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYASPDTSPEKLPDNSKAI 76
           M++++S NSE+   S+ ESPHSPLRFHSPL SDAGD PE+  Y SP+ SP K+ +N K+I
Sbjct: 3   MKRTASSNSEA--QSYNESPHSPLRFHSPL-SDAGDLPES-RYVSPEGSPFKI-ENPKSI 62

Query: 77  VVVDVVDKSTQFSPLPSPY--ADSRKPP---AENANFP----ADRSTSSKVMFNRAMREE 136
           V     +K TQFSPLPSP      + PP     NA  P    +D+S SS V+ N  +RE+
Sbjct: 63  V---AGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVRED 122

Query: 137 VPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVCEVAVCLI 196
             Q+ T+   A   G E                  R    D ++  ALGFR+ EV +C+I
Sbjct: 123 GGQTTTRKAGAPINGEE-----------STRTTVNRARGDDLVSLTALGFRITEVILCVI 182

Query: 197 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKKHMIRHYL 256
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VNVIAF+Y+AF+A D    + K+ +MI    
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGF 242

Query: 257 RYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLAFVAFAFS 316
              F F +DQ+LAYLL+S+SS AATRVDDW SNWGKDEFTQMATAS+++SFLAF AFA S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

Query: 317 SLISGYNLCT 318
           +LIS Y L T
Sbjct: 303 ALISSYRLFT 293

BLAST of MC06g0126 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-61
Identity = 144/277 (51.99%), Postives = 183/277 (66.06%), Query Frame = 0

Query: 53  PPETPPYASPDT-SPEKLP---DNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANF 112
           P +T P  SP + S EK P   D S AIV  D  + +T FS  PSP   S    +E+   
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVAFD--NSTTHFSSSPSP-PHSLDHSSESEKE 63

Query: 113 PADRSTSSKVMFNRAMREEVP----------QSVTKVGPAATGGVEGGGGGGGGRSPKQM 172
            A     S+   N    EE P          +SV +V P       G G   G RS    
Sbjct: 64  DAKSKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARVGSGRSSGQRSGAVS 123

Query: 173 APAPRRSKADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN 232
           A   R  + + +  +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN
Sbjct: 124 AILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVN 183

Query: 233 VIAFIYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQ 292
           V+AF+Y++FQA DL Y L+K+KH+I H+LR  F+F +DQVLAYLL+S+S++A TRVDDW 
Sbjct: 184 VVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWV 243

Query: 293 SNWGKDEFTQMATASVSMSFLAFVAFAFSSLISGYNL 316
           SNWGKDEFT+MA+AS++MSFLAF+AFAFSSLISGYNL
Sbjct: 244 SNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of MC06g0126 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 89/156 (57.05%), Postives = 112/156 (71.79%), Query Frame = 0

Query: 162 PRRSK-ADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVI 221
           PR SK A  + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  NVI
Sbjct: 110 PRESKWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVI 169

Query: 222 AFIYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSN 281
            F+Y+ F   DLVY L       RH LR+  +F +DQ+LAYLL S+S+SA+ RVDDWQSN
Sbjct: 170 GFVYSGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSN 229

Query: 282 WGKDEFTQMATASVSMSFLAFVAFAFSSLISGYNLC 317
           WG D+F  +A ASV++S+++FVAFAF SL SGY LC
Sbjct: 230 WGADKFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of MC06g0126 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 80.1 bits (196), Expect = 3.6e-15
Identity = 57/156 (36.54%), Postives = 90/156 (57.69%), Query Frame = 0

Query: 163 RRSKADALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAF 222
           R  + D + KA+   R   +   LI+F +M S+K  G+ G +F+ Y+EYRY L +++I+ 
Sbjct: 37  RWKREDLIKKASPITRGICLLFSLIAFLIMVSNK-HGY-GRNFNDYEEYRYVLAISIIST 96

Query: 223 IYAAFQAFDLVYTLIKKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWG 282
           +Y A+Q F        K+ +         DF  DQ++AYLLIS++SSA    + ++   G
Sbjct: 97  LYTAWQTF----AHFSKREIFDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFRE--G 156

Query: 283 KDE-FTQMATASVSMSFLAFVAFAFSSLISGYNLCT 318
           +D  FT  A +++SM+  AF+A A S+L SGY L T
Sbjct: 157 QDNIFTDSAASAISMAIFAFIALALSALFSGYKLST 184

BLAST of MC06g0126 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-12
Identity = 50/144 (34.72%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 179 VCEVAVCLISFSVMASDKTQ-GWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLI 238
           +C V+   ++ +V    K     +  SF  Y E  YC  V VI F+Y + Q F  V  + 
Sbjct: 66  LCFVSALSLAVNVQRPSKRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDIT 125

Query: 239 KKKHMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQM---ATASV 298
            +  +I   L  +  F  DQV+ YLL+SSSS A      W  +  +D    +   +  SV
Sbjct: 126 HRGVLISEPLSDYISFIFDQVICYLLVSSSSVAIA----WIQHINEDAIKTLRNNSIVSV 185

Query: 299 SMSFLAFVAFAFSSLISGYNLCTR 319
           SMSF AF+    S L+SGY LC R
Sbjct: 186 SMSFSAFLVLTLSGLLSGYKLCKR 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q501G61.5e-6652.90CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
D7MMW47.4e-6652.58CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q84WP53.6e-6051.99CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
D7LIR21.3e-5749.82CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q9FNE81.5e-4257.05CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022135555.11.78e-221100.00CASP-like protein 4A2 [Momordica charantia][more]
XP_038878399.17.65e-19690.74CASP-like protein 4A2 [Benincasa hispida][more]
XP_008453094.11.05e-19087.92PREDICTED: CASP-like protein 4A2 [Cucumis melo] >KAA0057869.1 CASP-like protein ... [more]
XP_031746040.11.52e-18685.63CASP-like protein 4A2 [Cucumis sativus][more]
XP_023530616.11.75e-18487.54CASP-like protein 4A2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1C1D18.64e-222100.00CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1[more]
A0A5D3BHR85.09e-19187.92CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00... [more]
A0A1S3BWI95.09e-19187.92CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103493912 PE=3 SV=1[more]
A0A0A0LNX37.35e-18785.63CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1[more]
A0A6J1E1W91.72e-18387.54CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G62820.11.1e-6752.90Uncharacterised protein family (UPF0497) [more]
AT2G36330.12.5e-6151.99Uncharacterised protein family (UPF0497) [more]
AT5G40300.11.1e-4357.05Uncharacterised protein family (UPF0497) [more]
AT2G38480.13.6e-1536.54Uncharacterised protein family (UPF0497) [more]
AT4G11655.11.7e-1234.72Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 169..303
e-value: 2.2E-32
score: 111.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..163
NoneNo IPR availablePANTHERPTHR11615:SF251CASP-LIKE PROTEINcoord: 74..319
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 74..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0126.1MC06g0126.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane