MC06g0010 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g0010
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMagnesium protoporphyrin IX methyltransferase
LocationMC06: 100799 .. 102808 (-)
RNA-Seq ExpressionMC06g0010
SyntenyMC06g0010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAAAGAAATTAAACCGAGACATTCGATGGCGACAACTGACGACCTTGTGAGGTTGACGGTTACGACCGTCACCCGTACACAGTGTTTACTCTAAAAAAATGGTAGAATTTGACGGTGAGGTGAGCGGCAAGGGTGGGTGTGGGACCCAAGAGAATCAGGGGAGGATAATCCCAAAAGGATGTGTGGATTTGGTTGGCCTTCGCCGGTCGAGATTAGAGATCAGAATTTATTTACACGTAGCCGAGGAAGCCATAGCCTCAGACTCAGTTCCCCCGGAACCATGGCTCACTCCGCGTCTTTATATCCCACACTTTGCTTCCACGCCGATTCTACTTCAAATTCCAAATCCAGATTGTTTCTGTGTCCACCCCCTCCCAAGCTCATTAAATTTCCCAGAACCCCACCGGTGTCCGCCGTGGCCACGGAACTCCCCCTCGTCGACGGGGCCACAATTGCAGCAATCGGTGGAGGTTCGGTTGCCGCATTGGCTGCCCTCGTATCTCTCACCGACCCGGAGAGACGCCGCCGTCTCCAGGCCGAAGAGGTGGGCGGCGTCGACAAGGATGTGGTGAGAGAATATTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATACGGAGAGACGGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGGCACTCCAAGACCGTGGAGAATGTGATGGCCATGTTGAAGGACGATGGGCCACTCGCAGGAGTAACAGTGTGTGATGCTGGCTGTGGAACCGGTAGCTTATCCATTCCCCTGGCTAAGGAAGGCGCCATCGTCTTCGCCAGCGACATTTCTGCTGCTATGGTCCTGGAAGCCCAAAACAAGGTGCAATTCCTCAATTTCTCAATTCTATTTTTGAACTGCTCAAGGATAAGATGCTGTTTTGAATAAGGATAAGGAAAGGATTGGTGTGCAAATACTTTGGTTAAATTGATTGTTACAATTAGGACGTTTATGCTGTTTTTGAACTGAGGTGAGTGGGGAGTGGTGCAGGCAAAAGAAGAGCTTGTAGGCGTGATGCCAGAATTTGAAGTGAGAGATTTGGAGAGCTTGGAGGGGAAGTATGATACGGTGGTATGTCTGGATGTTCTGATACACTACCCCCAGAACAAGGCAGACGGGATGATTGCGCACCTTGCATCGCTGGCTGAGAAGCGTTTGATCATGAGCTTTGCGCCAAAAACATTTTACTACGATCTCCTGAAGAGGGTGGGGGAGTTGTTCCCAGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCAGAGGTAGACGTAGAGACCGCACTGCGGAAAGTTGGGTGGAAAATAAGGAAGAGGGGTCTAATCACCACCCAGTTTTACTTTGCCAGATTGGTTGAGGCAGTTCCAGTTTGACGATTCACACCCCTTCTAGTACTCTACGTCTATTGTTCCCTTTGTATCACTATATCATTCTTCCTCTTCCTCAACTTATTACAACAACTATAATGCCGATTGCCGGATTTCGATTTACACTGCCTCCCCTCGTATCCATCTGAATGCAGTTTTTGTGTCACCTCAGGCTCACCCACCTCTTTTTCCTCTTCCCATTTTCCCTAACTTCGGGAGGATTGATTAATCTATCTTGTATTAATTGGCATTAGAGTTGGTTTTCAACATGGGGTGGATCTGCTATCAGTCTTCTGCACTCGCTCATTGAGCGTGTGTTATCAATTTCGTAAAGGATGCCTGCAAGCATGAAAACTGCACTGCTCTCAAACCAACCTTTCCTACGCCATAAAACTCTTCTACTTCTATGAGTTCGTTGATGTTGTGTGATCAATACCAACCAATTAATTGTAGGTATTTGTTATCACACAGTCGAGTTTCGTCAAGAAGGGTATGCACGAGACTAGGAACCATGGTATAAACAAAAATGTTGTTTTTCACTCATATAGAACATGATGATTTAGAAGATGAAAAATGCACCCTTGTGCTCAGTTGTTGTCCTATATTTGGAATCTTTC

mRNA sequence

GTAAAAAGAAATTAAACCGAGACATTCGATGGCGACAACTGACGACCTTGTGAGGTTGACGGTTACGACCGTCACCCGTACACAGTGTTTACTCTAAAAAAATGGTAGAATTTGACGGTGAGGTGAGCGGCAAGGGTGGGTGTGGGACCCAAGAGAATCAGGGGAGGATAATCCCAAAAGGATGTGTGGATTTGGTTGGCCTTCGCCGGTCGAGATTAGAGATCAGAATTTATTTACACGTAGCCGAGGAAGCCATAGCCTCAGACTCAGTTCCCCCGGAACCATGGCTCACTCCGCGTCTTTATATCCCACACTTTGCTTCCACGCCGATTCTACTTCAAATTCCAAATCCAGATTGTTTCTGTGTCCACCCCCTCCCAAGCTCATTAAATTTCCCAGAACCCCACCGGTGTCCGCCGTGGCCACGGAACTCCCCCTCGTCGACGGGGCCACAATTGCAGCAATCGGTGGAGGTTCGGTTGCCGCATTGGCTGCCCTCGTATCTCTCACCGACCCGGAGAGACGCCGCCGTCTCCAGGCCGAAGAGGTGGGCGGCGTCGACAAGGATGTGGTGAGAGAATATTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATACGGAGAGACGGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGGCACTCCAAGACCGTGGAGAATGTGATGGCCATGTTGAAGGACGATGGGCCACTCGCAGGAGTAACAGTGTGTGATGCTGGCTGTGGAACCGGTAGCTTATCCATTCCCCTGGCTAAGGAAGGCGCCATCGTCTTCGCCAGCGACATTTCTGCTGCTATGGTCCTGGAAGCCCAAAACAAGGCAAAAGAAGAGCTTGTAGGCGTGATGCCAGAATTTGAAGTGAGAGATTTGGAGAGCTTGGAGGGGAAGTATGATACGGTGGTATGTCTGGATGTTCTGATACACTACCCCCAGAACAAGGCAGACGGGATGATTGCGCACCTTGCATCGCTGGCTGAGAAGCGTTTGATCATGAGCTTTGCGCCAAAAACATTTTACTACGATCTCCTGAAGAGGGTGGGGGAGTTGTTCCCAGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCAGAGGTAGACGTAGAGACCGCACTGCGGAAAGTTGGGTGGAAAATAAGGAAGAGGGGTCTAATCACCACCCAGTTTTACTTTGCCAGATTGGTTGAGGCAGTTCCAGTTTGACGATTCACACCCCTTCTAGTACTCTACGTCTATTGTTCCCTTTGTATCACTATATCATTCTTCCTCTTCCTCAACTTATTACAACAACTATAATGCCGATTGCCGGATTTCGATTTACACTGCCTCCCCTCGTATCCATCTGAATGCAGTTTTTGTGTCACCTCAGGCTCACCCACCTCTTTTTCCTCTTCCCATTTTCCCTAACTTCGGGAGGATTGATTAATCTATCTTGTATTAATTGGCATTAGAGTTGGTTTTCAACATGGGGTGGATCTGCTATCAGTCTTCTGCACTCGCTCATTGAGCGTGTGTTATCAATTTCGTAAAGGATGCCTGCAAGCATGAAAACTGCACTGCTCTCAAACCAACCTTTCCTACGCCATAAAACTCTTCTACTTCTATGAGTTCGTTGATGTTGTGTGATCAATACCAACCAATTAATTGTAGGTATTTGTTATCACACAGTCGAGTTTCGTCAAGAAGGGTATGCACGAGACTAGGAACCATGGTATAAACAAAAATGTTGTTTTTCACTCATATAGAACATGATGATTTAGAAGATGAAAAATGCACCCTTGTGCTCAGTTGTTGTCCTATATTTGGAATCTTTC

Coding sequence (CDS)

ATGTGTGGATTTGGTTGGCCTTCGCCGGTCGAGATTAGAGATCAGAATTTATTTACACGTAGCCGAGGAAGCCATAGCCTCAGACTCAGTTCCCCCGGAACCATGGCTCACTCCGCGTCTTTATATCCCACACTTTGCTTCCACGCCGATTCTACTTCAAATTCCAAATCCAGATTGTTTCTGTGTCCACCCCCTCCCAAGCTCATTAAATTTCCCAGAACCCCACCGGTGTCCGCCGTGGCCACGGAACTCCCCCTCGTCGACGGGGCCACAATTGCAGCAATCGGTGGAGGTTCGGTTGCCGCATTGGCTGCCCTCGTATCTCTCACCGACCCGGAGAGACGCCGCCGTCTCCAGGCCGAAGAGGTGGGCGGCGTCGACAAGGATGTGGTGAGAGAATATTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATACGGAGAGACGGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGGCACTCCAAGACCGTGGAGAATGTGATGGCCATGTTGAAGGACGATGGGCCACTCGCAGGAGTAACAGTGTGTGATGCTGGCTGTGGAACCGGTAGCTTATCCATTCCCCTGGCTAAGGAAGGCGCCATCGTCTTCGCCAGCGACATTTCTGCTGCTATGGTCCTGGAAGCCCAAAACAAGGCAAAAGAAGAGCTTGTAGGCGTGATGCCAGAATTTGAAGTGAGAGATTTGGAGAGCTTGGAGGGGAAGTATGATACGGTGGTATGTCTGGATGTTCTGATACACTACCCCCAGAACAAGGCAGACGGGATGATTGCGCACCTTGCATCGCTGGCTGAGAAGCGTTTGATCATGAGCTTTGCGCCAAAAACATTTTACTACGATCTCCTGAAGAGGGTGGGGGAGTTGTTCCCAGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCAGAGGTAGACGTAGAGACCGCACTGCGGAAAGTTGGGTGGAAAATAAGGAAGAGGGGTCTAATCACCACCCAGTTTTACTTTGCCAGATTGGTTGAGGCAGTTCCAGTTTGA

Protein sequence

MCGFGWPSPVEIRDQNLFTRSRGSHSLRLSSPGTMAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVEAVPV
Homology
BLAST of MC06g0010 vs. ExPASy Swiss-Prot
Match: Q9SW18 (Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHLM PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.7e-121
Identity = 228/304 (75.00%), Postives = 255/304 (83.88%), Query Frame = 0

Query: 53  SNSKSRLFLCPPPPKLIKFPRTP------PVSAVATELPLVDGATIAAIGGGSVAALAAL 112
           S+S S     P  P   +F  TP       V+A  T+L  VD  TIA +GGGSVAALAA+
Sbjct: 9   SSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAM 68

Query: 113 VSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSK 172
           VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+K
Sbjct: 69  VSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAK 128

Query: 173 TVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKE 232
           TVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK 
Sbjct: 129 TVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKA 188

Query: 233 ELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAP 292
           +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAP
Sbjct: 189 QLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAP 248

Query: 293 KTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVE 350
           KTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+E
Sbjct: 249 KTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIE 308

BLAST of MC06g0010 vs. ExPASy Swiss-Prot
Match: Q55467 (Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=chlM PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-69
Identity = 130/222 (58.56%), Postives = 168/222 (75.68%), Query Frame = 0

Query: 127 DKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTV 186
           DK +VR+YFN++GF RW++IYG+   VN VQ+DIR+GH +TV++V+A L  DG L G+ V
Sbjct: 8   DKTIVRDYFNSTGFDRWRRIYGD-GQVNFVQKDIRVGHQQTVDSVVAWLVADGNLPGLLV 67

Query: 187 CDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKEELV-GVMPEFEVRDLESLEG 246
           CDAGCG GSLSIPLA+ GA+V+ SDIS  MV EAQ KA+E L  G  P F  +DL  L G
Sbjct: 68  CDAGCGVGSLSIPLAQAGALVYGSDISEKMVGEAQQKAQEVLAYGNQPTFMTQDLAQLGG 127

Query: 247 KYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSK 306
           KYDTV+CLDVLIHYP  +A  MI+HLASLA++RLI+SFAPKT    +LK++G LFPGPSK
Sbjct: 128 KYDTVICLDVLIHYPTEEASAMISHLASLADRRLILSFAPKTLGLTVLKKIGGLFPGPSK 187

Query: 307 ATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVEAV 348
            TRAY H E D+   L   G+ I + G+ +T+FY++R++EAV
Sbjct: 188 TTRAYQHKEADIRKILGDNGFSIARTGMTSTRFYYSRILEAV 228

BLAST of MC06g0010 vs. ExPASy Swiss-Prot
Match: P26236 (Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 GN=bchM PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.8e-17
Identity = 62/219 (28.31%), Postives = 109/219 (49.77%), Query Frame = 0

Query: 131 VREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAG 190
           V  YF+ +  + W ++    + V++V++ +R G       +++ L DD  L G  V DAG
Sbjct: 12  VEHYFDRTATRAWARLTTADEKVSKVRQTVREGRDTMRAVMLSRLPDD--LTGCRVMDAG 71

Query: 191 CGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKEELVGVMPEFEVRDL-ESLEGKYDT 250
           CGTG  ++ LA+ GA V A DIS  ++  A+++   EL G +  F V D+ +   G++D 
Sbjct: 72  CGTGLTTVELARRGADVVAVDISPQLIDIAKDRLPPELRGKV-SFHVGDMADPALGQFDY 131

Query: 251 VVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRA 310
           VV +D LI+Y       ++  L       ++ + APKT +      +G+LFP  +++   
Sbjct: 132 VVAMDSLIYYRAPDIGRVLTELGKRTHSAIVFTVAPKTAFLMAFWWLGKLFPRSNRSPVM 191

Query: 311 YLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVEAVP 349
             H+   ++   R  G  + K   +   FY +  +E  P
Sbjct: 192 IPHA---LDKLQRHAGDSLIKIDRVARGFYISECLEYRP 224

BLAST of MC06g0010 vs. ExPASy Swiss-Prot
Match: Q9PAM5 (Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain 9a5c) OX=160492 GN=ubiG PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.1e-05
Identity = 39/123 (31.71%), Postives = 61/123 (49.59%), Query Frame = 0

Query: 180 PLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKEELVGVMPEFEVRD 239
           PL+G  V D GCG G LS  LA++GA V A D++  ++  A+    E   G+  ++ ++ 
Sbjct: 53  PLSGARVLDVGCGGGLLSEALARQGAHVTAIDLAPELIKVARLHGLES--GIQVDYRIQA 112

Query: 240 LESL----EGKYDTVVCLDVLIHYPQNKA-DGMIAHLASLAEKRLIMSFAPKTFYYDLLK 298
           +E L       +D + C+++L H P   A     AHL      RL +S   +T    +L 
Sbjct: 113 IEDLLAEQPAPFDAIACMEMLEHVPDPAAIVDACAHLLK-PGGRLFVSTINRTLAAFMLA 172

BLAST of MC06g0010 vs. ExPASy Swiss-Prot
Match: B0U3W1 (Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain M12) OX=405440 GN=ubiG PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.0e-04
Identity = 38/122 (31.15%), Postives = 60/122 (49.18%), Query Frame = 0

Query: 181 LAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKEELVGVMPEFEVRDL 240
           L+G  V D GCG G LS  LA++GA V A D++  ++  A+    E   G+  ++ ++ +
Sbjct: 54  LSGARVLDVGCGGGLLSEALARQGAHVTAIDLAPELIKAARLHGLES--GIQVDYRIQAI 113

Query: 241 ESL----EGKYDTVVCLDVLIHYPQNKA-DGMIAHLASLAEKRLIMSFAPKTFYYDLLKR 298
           E L       +D + C+++L H P   A     AHL      RL +S   +T    +L  
Sbjct: 114 EDLLAEQPAPFDAIACMEMLEHVPDPAAIVDACAHLLK-PGGRLFVSTINRTLAAFMLAV 172

BLAST of MC06g0010 vs. NCBI nr
Match: XP_022135547.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Momordica charantia])

HSP 1 Score: 621 bits (1601), Expect = 1.60e-223
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA 94
           MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA
Sbjct: 1   MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA 60

Query: 95  IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN 154
           IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN
Sbjct: 61  IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN 120

Query: 155 RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA 214
           RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA
Sbjct: 121 RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA 180

Query: 215 AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL 274
           AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL
Sbjct: 181 AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL 240

Query: 275 AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI 334
           AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI
Sbjct: 241 AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI 300

Query: 335 TTQFYFARLVEAVPV 349
           TTQFYFARLVEAVPV
Sbjct: 301 TTQFYFARLVEAVPV 315

BLAST of MC06g0010 vs. NCBI nr
Match: XP_038880651.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 549 bits (1414), Expect = 6.18e-195
Identity = 285/322 (88.51%), Postives = 298/322 (92.55%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRT-------PPVSAVATELPLV 94
           MAHSAS YPTLCFH DSTSNS  R FL P P KL+KF RT       PP+SAVATELP+V
Sbjct: 1   MAHSASPYPTLCFHPDSTSNS--RFFLSPTPIKLVKFSRTLNVNVPAPPLSAVATELPVV 60

Query: 95  DGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIY 154
           DGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKKIY
Sbjct: 61  DGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIY 120

Query: 155 GETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIV 214
           GETDDVNRVQRDIRLGHSKTVENVMAMLKD+GPL GVTVCDAGCGTGSLSIPLAKEGAIV
Sbjct: 121 GETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIV 180

Query: 215 FASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADG 274
           FASDISAAMV EAQNKAKEE+VG VMP+FEV+DLESLEGKYDTVVCLDVLIHYPQNKADG
Sbjct: 181 FASDISAAMVAEAQNKAKEEVVGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADG 240

Query: 275 MIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGW 334
           MIAHLASLA KRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVE ALR+VGW
Sbjct: 241 MIAHLASLAGKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVEAALRRVGW 300

Query: 335 KIRKRGLITTQFYFARLVEAVP 348
           KIRKRGLI+TQFYFA LVEAVP
Sbjct: 301 KIRKRGLISTQFYFATLVEAVP 320

BLAST of MC06g0010 vs. NCBI nr
Match: XP_022987155.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 548 bits (1411), Expect = 1.90e-194
Identity = 283/324 (87.35%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTP---------PVSAVATELP 94
           MAHSA  YPTLCFH DST+NS  R FL PPP KL+KF RTP         P+SAVATELP
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNS--RFFLSPPPLKLVKFSRTPNVNAPAPAPPLSAVATELP 60

Query: 95  LVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKK 154
           +VDGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKK
Sbjct: 61  VVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKK 120

Query: 155 IYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGA 214
           IYGETDDVNRVQRDIRLGHSKTVENVM MLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA
Sbjct: 121 IYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGA 180

Query: 215 IVFASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKA 274
           IVFASDISAAMV EAQNKAKEE+ G V+P+FEV+DLESLEGKYDTVVCLDVLIHYPQNKA
Sbjct: 181 IVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKA 240

Query: 275 DGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKV 334
           DGMIAHLASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+V
Sbjct: 241 DGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRV 300

Query: 335 GWKIRKRGLITTQFYFARLVEAVP 348
           GWKIRKRGLI+TQFYFA LVEAVP
Sbjct: 301 GWKIRKRGLISTQFYFATLVEAVP 322

BLAST of MC06g0010 vs. NCBI nr
Match: XP_023516229.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6589339.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023025.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547 bits (1410), Expect = 2.70e-194
Identity = 282/324 (87.04%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTP---------PVSAVATELP 94
           MAHSA  YPTLCFH DST+NS  R FL PPP KL+KF RTP         P+SAVATELP
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNS--RFFLSPPPLKLVKFSRTPNVNAPAPAPPLSAVATELP 60

Query: 95  LVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKK 154
           +VDGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKK
Sbjct: 61  VVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKK 120

Query: 155 IYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGA 214
           IYGETDDVNRVQRDIRLGHSKTVENVM MLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA
Sbjct: 121 IYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGA 180

Query: 215 IVFASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKA 274
           IVFASDISAAMV EAQNKAKEE+ G V+P+FEV+DLESLEGKYDTVVCLDVLIHYPQNKA
Sbjct: 181 IVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKA 240

Query: 275 DGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKV 334
           DGMIAHLASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+V
Sbjct: 241 DGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRV 300

Query: 335 GWKIRKRGLITTQFYFARLVEAVP 348
           GWKIRKRGL++TQFYFA LVEAVP
Sbjct: 301 GWKIRKRGLVSTQFYFATLVEAVP 322

BLAST of MC06g0010 vs. NCBI nr
Match: XP_022922008.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 545 bits (1405), Expect = 1.56e-193
Identity = 281/324 (86.73%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTP---------PVSAVATELP 94
           MAHSA  YPTLCFH DST+N   R FL PPP KL+KF RTP         P+SAVATELP
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNP--RFFLSPPPLKLVKFSRTPNVNAPAPAPPLSAVATELP 60

Query: 95  LVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKK 154
           +VDGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKK
Sbjct: 61  VVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKK 120

Query: 155 IYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGA 214
           IYGETDDVNRVQRDIRLGHSKTVENVM MLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA
Sbjct: 121 IYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGA 180

Query: 215 IVFASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKA 274
           IVFASDISAAMV EAQNKAKEE+ G V+P+FEV+DLESLEGKYDTVVCLDVLIHYPQNKA
Sbjct: 181 IVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKA 240

Query: 275 DGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKV 334
           DGMIAHLASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+V
Sbjct: 241 DGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRV 300

Query: 335 GWKIRKRGLITTQFYFARLVEAVP 348
           GWKIRKRGL++TQFYFA LVEAVP
Sbjct: 301 GWKIRKRGLVSTQFYFATLVEAVP 322

BLAST of MC06g0010 vs. ExPASy TrEMBL
Match: A0A6J1C302 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007475 PE=4 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 7.75e-224
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA 94
           MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA
Sbjct: 1   MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPPVSAVATELPLVDGATIAA 60

Query: 95  IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN 154
           IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN
Sbjct: 61  IGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVN 120

Query: 155 RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA 214
           RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA
Sbjct: 121 RVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISA 180

Query: 215 AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL 274
           AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL
Sbjct: 181 AMVLEAQNKAKEELVGVMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASL 240

Query: 275 AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI 334
           AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI
Sbjct: 241 AEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLI 300

Query: 335 TTQFYFARLVEAVPV 349
           TTQFYFARLVEAVPV
Sbjct: 301 TTQFYFARLVEAVPV 315

BLAST of MC06g0010 vs. ExPASy TrEMBL
Match: A0A6J1JG16 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484791 PE=4 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 9.21e-195
Identity = 283/324 (87.35%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTP---------PVSAVATELP 94
           MAHSA  YPTLCFH DST+NS  R FL PPP KL+KF RTP         P+SAVATELP
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNS--RFFLSPPPLKLVKFSRTPNVNAPAPAPPLSAVATELP 60

Query: 95  LVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKK 154
           +VDGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKK
Sbjct: 61  VVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKK 120

Query: 155 IYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGA 214
           IYGETDDVNRVQRDIRLGHSKTVENVM MLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA
Sbjct: 121 IYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGA 180

Query: 215 IVFASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKA 274
           IVFASDISAAMV EAQNKAKEE+ G V+P+FEV+DLESLEGKYDTVVCLDVLIHYPQNKA
Sbjct: 181 IVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKA 240

Query: 275 DGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKV 334
           DGMIAHLASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+V
Sbjct: 241 DGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRV 300

Query: 335 GWKIRKRGLITTQFYFARLVEAVP 348
           GWKIRKRGLI+TQFYFA LVEAVP
Sbjct: 301 GWKIRKRGLISTQFYFATLVEAVP 322

BLAST of MC06g0010 vs. ExPASy TrEMBL
Match: A0A6J1E5D5 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430090 PE=4 SV=1)

HSP 1 Score: 545 bits (1405), Expect = 7.55e-194
Identity = 281/324 (86.73%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTP---------PVSAVATELP 94
           MAHSA  YPTLCFH DST+N   R FL PPP KL+KF RTP         P+SAVATELP
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNP--RFFLSPPPLKLVKFSRTPNVNAPAPAPPLSAVATELP 60

Query: 95  LVDGATIAAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKK 154
           +VDGATIAAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKK
Sbjct: 61  VVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKK 120

Query: 155 IYGETDDVNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGA 214
           IYGETDDVNRVQRDIRLGHSKTVENVM MLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA
Sbjct: 121 IYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGA 180

Query: 215 IVFASDISAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKA 274
           IVFASDISAAMV EAQNKAKEE+ G V+P+FEV+DLESLEGKYDTVVCLDVLIHYPQNKA
Sbjct: 181 IVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKA 240

Query: 275 DGMIAHLASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKV 334
           DGMIAHLASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+V
Sbjct: 241 DGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRV 300

Query: 335 GWKIRKRGLITTQFYFARLVEAVP 348
           GWKIRKRGL++TQFYFA LVEAVP
Sbjct: 301 GWKIRKRGLVSTQFYFATLVEAVP 322

BLAST of MC06g0010 vs. ExPASy TrEMBL
Match: A0A5D3BF92 (Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00080 PE=4 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 2.27e-191
Identity = 282/317 (88.96%), Postives = 295/317 (93.06%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPP--VSAVATELPLVDGATI 94
           M+HSAS Y TLCFH DSTSNS  R FL P P KLIKF RT    VSAVATELP+VDGATI
Sbjct: 1   MSHSASRYLTLCFHPDSTSNS--RFFLSPTPIKLIKFSRTLNLNVSAVATELPIVDGATI 60

Query: 95  AAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDD 154
           AAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKKIYGETDD
Sbjct: 61  AAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDD 120

Query: 155 VNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDI 214
           VNRVQRDIRLGHSKTVENVMAMLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA VFASDI
Sbjct: 121 VNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKEGAKVFASDI 180

Query: 215 SAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL 274
           SAAMV EA+NKAKEE+ G VMP+FEV+DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL
Sbjct: 181 SAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL 240

Query: 275 ASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKR 334
           ASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+VGWKIRKR
Sbjct: 241 ASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKR 300

Query: 335 GLITTQFYFARLVEAVP 348
           GLI+TQFYFA LVEAVP
Sbjct: 301 GLISTQFYFATLVEAVP 315

BLAST of MC06g0010 vs. ExPASy TrEMBL
Match: E5GBL6 (Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 2.27e-191
Identity = 282/317 (88.96%), Postives = 295/317 (93.06%), Query Frame = 0

Query: 35  MAHSASLYPTLCFHADSTSNSKSRLFLCPPPPKLIKFPRTPP--VSAVATELPLVDGATI 94
           M+HSAS Y TLCFH DSTSNS  R FL P P KLIKF RT    VSAVATELP+VDGATI
Sbjct: 1   MSHSASRYLTLCFHPDSTSNS--RFFLSPTPIKLIKFSRTLNLNVSAVATELPIVDGATI 60

Query: 95  AAIGGGSVAALAALVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDD 154
           AAIGGGSVAALAA++SLTDPERRRRLQAEEVGG DKDVV+EYFNNSGFQRWKKIYGETDD
Sbjct: 61  AAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDD 120

Query: 155 VNRVQRDIRLGHSKTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDI 214
           VNRVQRDIRLGHSKTVENVMAMLKD+GPL GVTVCDAGCGTGSLSIPLAKEGA VFASDI
Sbjct: 121 VNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKEGAKVFASDI 180

Query: 215 SAAMVLEAQNKAKEELVG-VMPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL 274
           SAAMV EA+NKAKEE+ G VMP+FEV+DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL
Sbjct: 181 SAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHL 240

Query: 275 ASLAEKRLIMSFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKR 334
           ASLAEKRLI+SFAPKTFYYDLLKRVGELFPGPSKATRAYLHSE DVETALR+VGWKIRKR
Sbjct: 241 ASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKR 300

Query: 335 GLITTQFYFARLVEAVP 348
           GLI+TQFYFA LVEAVP
Sbjct: 301 GLISTQFYFATLVEAVP 315

BLAST of MC06g0010 vs. TAIR 10
Match: AT4G25080.3 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 435.3 bits (1118), Expect = 4.8e-122
Identity = 228/304 (75.00%), Postives = 255/304 (83.88%), Query Frame = 0

Query: 53  SNSKSRLFLCPPPPKLIKFPRTP------PVSAVATELPLVDGATIAAIGGGSVAALAAL 112
           S+S S     P  P   +F  TP       V+A  T+L  VD  TIA +GGGSVAALAA+
Sbjct: 9   SSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAM 68

Query: 113 VSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSK 172
           VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+K
Sbjct: 69  VSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAK 128

Query: 173 TVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKE 232
           TVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK 
Sbjct: 129 TVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKA 188

Query: 233 ELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAP 292
           +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAP
Sbjct: 189 QLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAP 248

Query: 293 KTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVE 350
           KTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+E
Sbjct: 249 KTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIE 308

BLAST of MC06g0010 vs. TAIR 10
Match: AT4G25080.1 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 435.3 bits (1118), Expect = 4.8e-122
Identity = 228/304 (75.00%), Postives = 255/304 (83.88%), Query Frame = 0

Query: 53  SNSKSRLFLCPPPPKLIKFPRTP------PVSAVATELPLVDGATIAAIGGGSVAALAAL 112
           S+S S     P  P   +F  TP       V+A  T+L  VD  TIA +GGGSVAALAA+
Sbjct: 9   SSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAM 68

Query: 113 VSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSK 172
           VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+K
Sbjct: 69  VSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAK 128

Query: 173 TVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKE 232
           TVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK 
Sbjct: 129 TVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKA 188

Query: 233 ELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAP 292
           +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAP
Sbjct: 189 QLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAP 248

Query: 293 KTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVE 350
           KTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+E
Sbjct: 249 KTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIE 308

BLAST of MC06g0010 vs. TAIR 10
Match: AT4G25080.2 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 435.3 bits (1118), Expect = 4.8e-122
Identity = 228/304 (75.00%), Postives = 255/304 (83.88%), Query Frame = 0

Query: 53  SNSKSRLFLCPPPPKLIKFPRTP------PVSAVATELPLVDGATIAAIGGGSVAALAAL 112
           S+S S     P  P   +F  TP       V+A  T+L  VD  TIA +GGGSVAALAA+
Sbjct: 9   SSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAM 68

Query: 113 VSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSK 172
           VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+K
Sbjct: 69  VSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAK 128

Query: 173 TVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKE 232
           TVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK 
Sbjct: 129 TVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKA 188

Query: 233 ELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAP 292
           +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAP
Sbjct: 189 QLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAP 248

Query: 293 KTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVE 350
           KTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+E
Sbjct: 249 KTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIE 308

BLAST of MC06g0010 vs. TAIR 10
Match: AT4G25080.5 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 435.3 bits (1118), Expect = 4.8e-122
Identity = 228/304 (75.00%), Postives = 255/304 (83.88%), Query Frame = 0

Query: 53  SNSKSRLFLCPPPPKLIKFPRTP------PVSAVATELPLVDGATIAAIGGGSVAALAAL 112
           S+S S     P  P   +F  TP       V+A  T+L  VD  TIA +GGGSVAALAA+
Sbjct: 9   SSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAM 68

Query: 113 VSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSK 172
           VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+K
Sbjct: 69  VSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAK 128

Query: 173 TVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAKE 232
           TVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK 
Sbjct: 129 TVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKA 188

Query: 233 ELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFAP 292
           +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAP
Sbjct: 189 QLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAP 248

Query: 293 KTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLVE 350
           KTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+E
Sbjct: 249 KTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIE 308

BLAST of MC06g0010 vs. TAIR 10
Match: AT4G25080.4 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 405.6 bits (1041), Expect = 4.0e-113
Identity = 201/245 (82.04%), Postives = 223/245 (91.02%), Query Frame = 0

Query: 106 LVSLTDPERRRRLQAEEVGGVDKDVVREYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHS 165
           +VSLTDPERRR+LQAEEVGG DK+VVREYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+
Sbjct: 1   MVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHA 60

Query: 166 KTVENVMAMLKDDGPLAGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVLEAQNKAK 225
           KTVEN M ML +D  LAGVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMV EA+ KAK
Sbjct: 61  KTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAK 120

Query: 226 EELVGV-MPEFEVRDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIMSFA 285
            +L    +P+FEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFA
Sbjct: 121 AQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFA 180

Query: 286 PKTFYYDLLKRVGELFPGPSKATRAYLHSEVDVETALRKVGWKIRKRGLITTQFYFARLV 345
           PKTFYYD+LKR+GELFPGPSKATRAYLHSE DVE AL KVGWKI KRGL TTQFYF+RL+
Sbjct: 181 PKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLI 240

Query: 346 EAVPV 350
           EAVP+
Sbjct: 241 EAVPM 245

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SW186.7e-12175.00Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thal... [more]
Q554675.9e-6958.56Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 68... [more]
P262361.8e-1728.31Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 G... [more]
Q9PAM52.1e-0531.71Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain 9a5c) ... [more]
B0U3W11.0e-0431.15Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain M12) O... [more]
Match NameE-valueIdentityDescription
XP_022135547.11.60e-223100.00magnesium protoporphyrin IX methyltransferase, chloroplastic [Momordica charanti... [more]
XP_038880651.16.18e-19588.51magnesium protoporphyrin IX methyltransferase, chloroplastic [Benincasa hispida][more]
XP_022987155.11.90e-19487.35magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima][more]
XP_023516229.12.70e-19487.04magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo sub... [more]
XP_022922008.11.56e-19386.73magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
A0A6J1C3027.75e-224100.00magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Momordica charan... [more]
A0A6J1JG169.21e-19587.35magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima... [more]
A0A6J1E5D57.55e-19486.73magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita moscha... [more]
A0A5D3BF922.27e-19188.96Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=119... [more]
E5GBL62.27e-19188.96Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=4126... [more]
Match NameE-valueIdentityDescription
AT4G25080.34.8e-12275.00magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.14.8e-12275.00magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.24.8e-12275.00magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.54.8e-12275.00magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.44.0e-11382.04magnesium-protoporphyrin IX methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010940Magnesium-protoporphyrin IX methyltransferase, C-terminalPFAMPF07109Mg-por_mtran_Ccoord: 252..347
e-value: 1.5E-34
score: 118.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 125..348
e-value: 1.9E-64
score: 219.2
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 92..345
NoneNo IPR availablePANTHERPTHR43591:SF32MAGNESIUM PROTOPORPHYRIN IX METHYLTRANSFERASE, CHLOROPLASTIC-RELATEDcoord: 92..345
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 185..265
e-value: 1.37453E-7
score: 47.4247
IPR010251Magnesium-protoporphyrin IX methyltransferaseTIGRFAMTIGR02021TIGR02021coord: 127..345
e-value: 7.7E-88
score: 291.5
IPR010251Magnesium-protoporphyrin IX methyltransferasePROSITEPS51556SAM_MT_MG_PIXcoord: 28..348
score: 105.70311
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 127..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g0010.1MC06g0010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0032259 methylation
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046406 magnesium protoporphyrin IX methyltransferase activity