MC06g0001 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g0001 vs. ExPASy Swiss-Prot
Match: P52780 (Glutamine--tRNA ligase OS=Lupinus luteus OX=3873 PE=2 SV=2)

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 615/795 (77.36%), Postives = 713/795 (89.69%), Query Frame = 0

Query: 1   MTMKND-NVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGN 60
           M  K+D + DK+K LE FL+IGLDERTA+NT+ANNKVT+NL +V+++AGV DGCSR VGN
Sbjct: 1   MPAKDDTSSDKEKSLELFLKIGLDERTAKNTVANNKVTTNLTSVINDAGVTDGCSRTVGN 60

Query: 61  LLYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACG 120
           LLYTVATKYPANAL HRP LLQYI+++K+K  AQL+AA SFLSA+G E++ LN+FE+ACG
Sbjct: 61  LLYTVATKYPANALPHRPTLLQYIVNSKVKTTAQLDAALSFLSATGSENLDLNKFEEACG 120

Query: 121 VGIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSK 180
           VG+EVS E+I+  V+EV EE+K  ILE RYR+NVG+L G VRK+ PWAD K+VKQ +D+K
Sbjct: 121 VGVEVSTEDIKHAVDEVVEENKATILELRYRVNVGELLGHVRKRLPWADAKVVKQLVDAK 180

Query: 181 LYGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKV 240
           LY +LG+RTAADNEK  KKKKEKP KV+ K     + E+P EEDLNPYL FP P+DN+KV
Sbjct: 181 LYEILGDRTAADNEK-PKKKKEKPAKVEDKAAPVATSEKPLEEDLNPYLIFPNPEDNFKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEV FS+G ILRCCNTK LLEKHLKATGG+ LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVPFSDGNILRCCNTKALLEKHLKATGGKVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           +R G CYLR+DDTNPEAEKKEYIDHIEEIV+WMGWEPFKITYTS+YFQ+LYE AV+LI  
Sbjct: 301 DRNGGCYLRFDDTNPEAEKKEYIDHIEEIVQWMGWEPFKITYTSNYFQELYEFAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQT +EIKEYREKK+NSPWRDRP++ESLKLF++M++G IE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTADEIKEYREKKLNSPWRDRPISESLKLFEDMRRGFIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DN+NMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HCIVDS+EN+THSLCTLEFETRRASY
Sbjct: 421 DNYNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCIVDSIENVTHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL +YQPYVWEYSRLNVSN V+SKRKLNRLVTEKWVDGWDDPRL+TLAGLRRRG+
Sbjct: 481 YWLLHALGIYQPYVWEYSRLNVSNTVMSKRKLNRLVTEKWVDGWDDPRLMTLAGLRRRGM 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           T TAINAFVRG+GITRSD +LI  +RLEYH+REELN++A RAMVVL PLKVVITNLE  S
Sbjct: 541 TPTAINAFVRGMGITRSDGTLISVERLEYHVREELNKTAPRAMVVLHPLKVVITNLEAKS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
            +++DAKKWP+AQAD+ASAFYK+PFSNVVYIE+SDFR+ DSKDYYGLAPGKSV+LRYA+P
Sbjct: 601 AIEVDAKKWPDAQADDASAFYKIPFSNVVYIERSDFRMQDSKDYYGLAPGKSVILRYAFP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCT+VILADD ET+LE+RAEYD SKK+KPKGVLHWV++PSPGVDPLKVEVRLF++LFLS
Sbjct: 661 IKCTEVILADDNETILEIRAEYDPSKKTKPKGVLHWVSQPSPGVDPLKVEVRLFERLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELD+WL DLNP SKV I +A+ V  L++A +GD FQFER+GYFAVD+DSTPEKL+F
Sbjct: 721 ENPAELDNWLGDLNPHSKVEISNAYGVSLLKDAKLGDRFQFERLGYFAVDQDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 794
           NRTVTL+D Y K  K
Sbjct: 781 NRTVTLKDSYGKGGK 794

BLAST of MC06g0001 vs. ExPASy Swiss-Prot
Match: Q8W4F3 (Glutamine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=OVA9 PE=2 SV=1)

HSP 1 Score: 1253.4 bits (3242), Expect = 0.0e+00
Identity = 606/797 (76.04%), Postives = 691/797 (86.70%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           M +K+DN   +K +E F+ IGLDE+TARNTI NNKVT+NL AV+HEA V DGC R  GNL
Sbjct: 1   MVLKDDN--SEKSIELFISIGLDEKTARNTINNNKVTANLTAVIHEAAVTDGCDRNTGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LY+VATK+P NALVHRP LL+YI+++KIK  AQLEAAF+F +++G ED KLNEFE+ACGV
Sbjct: 61  LYSVATKFPTNALVHRPTLLKYIVNSKIKTPAQLEAAFAFFASTGPEDFKLNEFEEACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           GIEVS E+IE+ V  +FEE+KK ILEQRYR NVG+LFG VRK  PWADPKIVK+ ID K+
Sbjct: 121 GIEVSPEDIEKAVKGIFEENKKTILEQRYRTNVGELFGHVRKSLPWADPKIVKKLIDEKM 180

Query: 181 YGLLGERTAADNEKTAKK--KKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYK 240
           Y LLGE+TAADNEK  KK  KKEKP KV+ K     +  +PSEE+LNPY  FPQP+ N+ 
Sbjct: 181 YELLGEKTAADNEKPTKKKEKKEKPAKVEEKKAVVETTAEPSEEELNPYTIFPQPEQNFM 240

Query: 241 VHTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLA 300
           VHTEVFFS+G+ILRC NTKE+L+KHLK TGG+  TRFPPEPNGYLHIGHAKAMFVDFGLA
Sbjct: 241 VHTEVFFSDGSILRCSNTKEVLDKHLKVTGGKVYTRFPPEPNGYLHIGHAKAMFVDFGLA 300

Query: 301 RERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLII 360
           +ERGG CYLRYDDTNPEAEK+EYI+HIEEIVKWMGWEPFKITYTSDYFQ+LY+LAV+LI 
Sbjct: 301 KERGGCCYLRYDDTNPEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLAVELIR 360

Query: 361 TGQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQ 420
            G AYVDHQT +EIKEYREKKMNSPWRDRP+ ESLKLFDEM++G+IE+GKATLRMKQDMQ
Sbjct: 361 RGHAYVDHQTADEIKEYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRMKQDMQ 420

Query: 421 SDNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRAS 480
           SDNFNMYDLIAYRIKF PHP AGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRAS
Sbjct: 421 SDNFNMYDLIAYRIKFAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRAS 480

Query: 481 YYWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRG 540
           YYWLLH+L LY PYVWEYSRLNV+N V+SKRKLN +VT K+VDGWDDPRLLTL+GLRRRG
Sbjct: 481 YYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRG 540

Query: 541 VTSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENG 600
           VTSTAINAFVRGIGITRSD S+I   RLE+HIREELN++A R MVVL PLKVVITNLE+ 
Sbjct: 541 VTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESD 600

Query: 601 SIMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAY 660
            +++LDAK+WP+AQ D+ SAFYKVPFS VVYI+QSDFR+ DSKDYYGLAPGKSVLLRYA+
Sbjct: 601 KLIELDAKRWPDAQNDDPSAFYKVPFSRVVYIDQSDFRMKDSKDYYGLAPGKSVLLRYAF 660

Query: 661 PIKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFL 720
           PIKCT+V+ ADD ETV E+ AEYD  KKSKPKGVLHWVAE SPG +P+KVEVRLF+KLF 
Sbjct: 661 PIKCTNVVFADDNETVREIHAEYDPEKKSKPKGVLHWVAESSPGEEPIKVEVRLFEKLFN 720

Query: 721 SENPAELDD-WLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKL 780
           SENPAEL+D WL D+NP SK+VI  A+AV  L++A VGD FQFER+GY+AVDKDS P KL
Sbjct: 721 SENPAELNDAWLTDINPNSKMVISGAYAVSTLKDAAVGDRFQFERLGYYAVDKDSEPGKL 780

Query: 781 IFNRTVTLRDGYNKSSK 794
           +FNRTVTLRD Y K  K
Sbjct: 781 VFNRTVTLRDSYGKGGK 795

BLAST of MC06g0001 vs. ExPASy Swiss-Prot
Match: P14325 (Probable glutamine--tRNA ligase OS=Dictyostelium discoideum OX=44689 GN=glnS PE=2 SV=2)

HSP 1 Score: 731.5 bits (1887), Expect = 1.0e-209
Identity = 380/792 (47.98%), Postives = 519/792 (65.53%), Query Frame = 0

Query: 8   VDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNLLYTVATK 67
           ++KD+L+  F +IGLD   A+ T  N  ++SNL  ++ EAG   GC ++VG LLYT+ATK
Sbjct: 7   INKDELVTLFSQIGLDSSKAKETTNNATLSSNLQEIIKEAGAESGCEKSVGLLLYTLATK 66

Query: 68  YPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGVGIEVSVE 127
           YPANA+ HR  L+ YI + K   +  L+A   +L  +  E++ + EFE +CGVG+ ++ E
Sbjct: 67  YPANAMKHRATLVDYIANKKSVNSINLQACLDYLRRTANEELNVAEFEQSCGVGVVITRE 126

Query: 128 EIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKLYGLLGER 187
           ++ Q V++   ++K ++LE+RY+ N+G +  +++    WA+ K +K+ +D+ +  LLG +
Sbjct: 127 QVAQAVSDYINKNKSDLLEKRYQFNIGGILMEIKNSLKWANAKDIKEEVDAAILSLLGPK 186

Query: 188 TAADNEKTAKK-KKEKPTKVDVKTVAT-----ISPEQPSE-EDLNPYLTFPQPQDNYKVH 247
           T AD    AK  K   PT V   T AT     +SP  P+E +     + FP P DN +  
Sbjct: 187 TDADKAPPAKPVKPTTPTAVATTTAATTTTGDLSPIIPAELKPAKEEIKFPDPSDNIQ-- 246

Query: 248 TEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARE 307
                         NT +LL  HLK TGG+ +TRFPPEPNGYLHIGHAKAM ++FG A++
Sbjct: 247 --------------NTPKLLADHLKTTGGKIVTRFPPEPNGYLHIGHAKAMHLNFGYAKK 306

Query: 308 RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG 367
            GG CYLR+DDTNPE E +EYID I + VKW+G EP +ITY+S  F  LYE+A +LI  G
Sbjct: 307 NGGKCYLRFDDTNPEKENQEYIDSIIDSVKWLGHEPCEITYSSSQFDTLYEMANELIRRG 366

Query: 368 QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD 427
            AYV HQT  EI E REK  +SP+R+R V E+LKLF++M+ G  E+GKA LRMK DM+  
Sbjct: 367 YAYVCHQTASEISEGREKMTDSPYRNRTVEENLKLFEDMRLGKFEEGKAILRMKGDMKHP 426

Query: 428 NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASYY 487
           N  M DLIAYRIK+  HP +GDKWCIYPSYDY HC+VDS+ENITHSLCTLEFE RR +Y 
Sbjct: 427 NPCMRDLIAYRIKYHHHPMSGDKWCIYPSYDYTHCLVDSIENITHSLCTLEFEIRRLTYN 486

Query: 488 WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT 547
           WL+  L LY+P VWEY+RLN+++ VLSKRK+  LV  K V+GWDDPRL TL   RR+G T
Sbjct: 487 WLIDVLGLYRPVVWEYARLNLTHTVLSKRKIITLVQNKIVNGWDDPRLSTLNAFRRKGYT 546

Query: 548 STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI 607
             AIN     IG+TR++ + I ++ LE   R++L+  A RAM V  P+KVVITN      
Sbjct: 547 PEAINLLCDTIGVTRTNGTTISYELLELCCRQDLDGKATRAMAVFDPIKVVITNYPEDKS 606

Query: 608 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 667
            +++A   P   +      +K+ FS +VYIE+SDFR+ D+KD++GLAPGK +LL+YAY I
Sbjct: 607 EEINA---PNIPSKPEKGTHKIDFSRIVYIERSDFRMEDNKDFFGLAPGKEILLKYAYNI 666

Query: 668 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 727
           KC  VI  D    V E+   YD    SK    +HWV+  + G +P+K EVRL++ LF   
Sbjct: 667 KCEKVI-QDADGKVTELHVTYDKDNSSKKLKTIHWVSSVA-GTEPMKAEVRLYEHLFKDS 726

Query: 728 NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIFN 787
                DDWL ++NP S  +IP+AF    +  +   D +QFER+GYF VDKD+T +K++FN
Sbjct: 727 EIG--DDWLNNINPNSLRIIPNAFIDKTVLASKEYDRYQFERVGYFVVDKDTTSDKMVFN 775

Query: 788 RTVTLRDGYNKS 792
           RTV+L++   KS
Sbjct: 787 RTVSLKENKEKS 775

BLAST of MC06g0001 vs. ExPASy Swiss-Prot
Match: Q9Y105 (Probable glutamine--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GlnRS PE=2 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 8.2e-167
Identity = 340/795 (42.77%), Postives = 488/795 (61.38%), Query Frame = 0

Query: 11  DKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNLLYTVATKYPA 70
           D L+ +F  +G+ E+ A+ T+ N  VT NL   +  AG     S   G L+Y +ATK   
Sbjct: 4   DDLIAKFQALGMSEQKAKETLKNANVTKNLQLSLAAAGSAT-LSDGTGMLIYHMATKLKP 63

Query: 71  NALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQE---DVKLNEFEDACGVGIEVSVE 130
               H P+L++YI+ +K+    +++AA  +L   GQ    ++ L   E  CGVG+ V+ E
Sbjct: 64  QTADHLPLLVRYIVEHKLDNTQRVDAALEYLLKCGQSLNANIDLQALEKECGVGVVVTPE 123

Query: 131 EIEQTVN-EVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKLYGLLGE 190
           +IE+TV  ++   +K+ +LEQRY  N   +   VR +  WAD K VK  ID +++ LLG 
Sbjct: 124 QIERTVQAKIKASYKEALLEQRYHFNSFKILQDVRGELKWADAKSVKAAIDVEIFDLLGP 183

Query: 191 RTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH----TE 250
           +T AD  K   K  +KP     K  A ++P   + E  +   T        KVH     E
Sbjct: 184 KTEAD-LKPQTKANDKPKA--AKPKAEVTPAAQTAEAASDGATTISELMKTKVHFHAPGE 243

Query: 251 VFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARERG 310
            F ++G ++   +T+ LL++HL  TGG+  TRFPPEPNG LHIGHAKA+ ++FG A    
Sbjct: 244 NFKADGYVV-TEHTERLLKEHLARTGGKVHTRFPPEPNGILHIGHAKAININFGYAAAHD 303

Query: 311 GSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITGQA 370
           G CYLRYDDTNPE E++++   I+E+V+W+G++PFKITY+SD FQ LYE AV LI  G A
Sbjct: 304 GVCYLRYDDTNPEKEEEKFFLAIKEMVEWLGYKPFKITYSSDNFQQLYEWAVVLINKGLA 363

Query: 371 YVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSDNF 430
           YV HQ  EE+K +  K   SPWR+RP+ ESL+LF++MK+G I++G ATLRMK  ++    
Sbjct: 364 YVCHQKAEELKGFNPKP--SPWRERPIEESLRLFEDMKRGKIDEGAATLRMKVTLEEGKM 423

Query: 431 NMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASYYWL 490
              D +AYRIKF  H   G  WCIYP+YDY HC+ DSLE+ITHSLCT EF++RR+SYYWL
Sbjct: 424 ---DPVAYRIKFISHHRTGSDWCIYPTYDYTHCLCDSLEDITHSLCTKEFQSRRSSYYWL 483

Query: 491 LHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVTST 550
            +AL +Y P  WEY RLN++  ++SKRK+ +L+TE+ V  WDDPRL TL  LRRRG  + 
Sbjct: 484 CNALGIYCPVQWEYGRLNMNYALVSKRKIAKLITEQIVHDWDDPRLFTLTALRRRGFPAE 543

Query: 551 AINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSIMD 610
           AIN F   +G+T +  + +    LE  +R+ LN +A R +VVL+PLKV I N  + + + 
Sbjct: 544 AINNFCAQMGVTGAQIA-VDPAMLEAAVRDVLNVTAPRRLVVLEPLKVTIKNFPHAAPVQ 603

Query: 611 LDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPIKC 670
           L+   +P+   +     +K+    V+YIEQ DF+L   K Y  LAP +SV LR+A  +  
Sbjct: 604 LEVPDFPQ---NPQQGTHKITLDKVIYIEQGDFKLEPEKGYRRLAPKQSVGLRHAGLVIS 663

Query: 671 TDVILADDK--ETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 730
            D I+ D    + V  +     A +  KPK  + WV++      P+++EVRL+++LF  +
Sbjct: 664 VDEIVKDPATGQVVELICTSQPAEQAEKPKAFVQWVSQ------PIQLEVRLYEQLFKHK 723

Query: 731 NPAELDD----WLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEK 790
           NP + ++    +L+D++ QS  V+  AFA   L  A V D FQFERIG+F+VD D++   
Sbjct: 724 NPEDPNEVPGGFLSDISEQSMSVV-VAFADRALNQAKVYDKFQFERIGFFSVDPDTSANH 777

BLAST of MC06g0001 vs. ExPASy Swiss-Prot
Match: O62431 (Probable glutamine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=qars-1 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 3.5e-165
Identity = 324/793 (40.86%), Postives = 485/793 (61.16%), Query Frame = 0

Query: 15  EQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNLLYTVATKYPANALV 74
           E+ L +GL +     T+ N K+T  + ++V  A      S+  G LLY +ATK       
Sbjct: 5   EELLSLGLSDSKVAETLKNVKLTETIGSIVKLASESGEISKQKGTLLYQLATKLKPQVAA 64

Query: 75  HRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGVGIEVSVEEIEQTVN 134
           H P++++YI+++ IK   QL AA  +L +   + +++ +FE +CGVG+ V++++IE  V 
Sbjct: 65  HTPLVVKYIMNDGIKTEPQLSAAIEYLLSHTVKGIQVPDFEKSCGVGVVVTIDDIEAAVT 124

Query: 135 EVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKLYGLLGERTAAD-NE 194
           +V  +H+++I+ +RY    G L G++R   PWAD  I K+ +D +   LLG +TA D   
Sbjct: 125 KVIGQHREKIVAERYSFPAGKLLGELRALLPWADGAITKKEVDLRFLELLGPKTAEDLAP 184

Query: 195 KTAKKKKEKPTKVDVKTVATISPEQPSEEDLNP--YLTFPQPQDN--------YKVHTEV 254
           K  +KK E P        A  +P   ++++ +P  +    +  D         +KV    
Sbjct: 185 KKKEKKPEGPKPSKDAAAAATAPGTKNQKEASPEEFADGAETMDELLRTRAHFHKVGEN- 244

Query: 255 FFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARERGG 314
            F     +    T ELL+ H+ A GG+ +TRFPPEPNG LHIGHAKA+ ++FG A+  GG
Sbjct: 245 -FKQDGYVTTPKTAELLKAHVAAVGGKVVTRFPPEPNGVLHIGHAKAININFGYAKAMGG 304

Query: 315 SCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITGQAY 374
            C LR+DDTNPE E++++   IE+IV W+G++P ++T++SD FQ LY  AVKLI  G A+
Sbjct: 305 VCNLRFDDTNPEKEEEKFFSAIEDIVHWLGYDPARVTHSSDNFQQLYLWAVKLIQKGLAF 364

Query: 375 VDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSDNFN 434
           V HQ  EE++ +  +   SPWR+RP+ E+++LF++MK G  ++G+ATLR+K  ++     
Sbjct: 365 VCHQKVEEMRGFEVQL--SPWRERPIEENIQLFEDMKNGKFDEGEATLRLKLTLEE---G 424

Query: 435 MYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASYYWLL 494
             D +AYRIK+ PH   G++WCIYP+YDY HC+ DS+ENITHSLCT EF++RR+SYYWL 
Sbjct: 425 KVDPVAYRIKYVPHHRTGNQWCIYPTYDYTHCLCDSIENITHSLCTKEFQSRRSSYYWLC 484

Query: 495 HALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVTSTA 554
           +AL++Y P  WEY RLNV+  V+SKRK+ +L+T K V+ WDDPRL TL  LRRRG+ S A
Sbjct: 485 NALDIYCPVQWEYGRLNVNYTVVSKRKILKLITTKTVNDWDDPRLFTLTALRRRGIPSEA 544

Query: 555 INAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI-MD 614
           IN FV  +G+T S   +I    L+  +R+ LN  A R M VL+ LK+ I N    ++   
Sbjct: 545 INRFVAKLGLTMSQ-MVIDPHVLDATVRDYLNIHAPRTMAVLEGLKLTIENFSELNLPSS 604

Query: 615 LDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDS-KDYYGLAPGKSVLLRYAYPIK 674
           +D   +P    D     + V     ++IE+SD++ +DS K +  L P ++V L++   + 
Sbjct: 605 VDVPDFPSDPTDPRK--HSVSVDREIFIEKSDYKPDDSDKSFRRLTPKQAVGLKHIGLVL 664

Query: 675 --CTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 734
               +V  A+   T + V+AE   S+K KPK  +HWVA+      P+  EVRL+D+LF S
Sbjct: 665 RFVKEVKDAEGHVTEVVVKAE-KLSEKDKPKAFIHWVAK------PVSCEVRLYDRLFKS 724

Query: 735 ENPAELD----DWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPE 788
           +NP +       +L+D+NP S  V+ +A     +  + V D FQFERIG+F VD+DST  
Sbjct: 725 KNPEDAQLVPGGFLSDINPDSLTVVYNALIDQSIAKSKVYDRFQFERIGFFCVDRDSTSS 780

BLAST of MC06g0001 vs. NCBI nr
Match: XP_022134744.1 (glutamine--tRNA ligase [Momordica charantia])

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 763/766 (99.61%), Postives = 763/766 (99.61%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL
Sbjct: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV
Sbjct: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL
Sbjct: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH 240
           YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH
Sbjct: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH 240

Query: 241 TEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARE 300
           TEVFFSNGTILRCCNTKELLEKHLKATGGR LTRFPPEPNGYLHIGHAKAMFVDFGLARE
Sbjct: 241 TEVFFSNGTILRCCNTKELLEKHLKATGGRVLTRFPPEPNGYLHIGHAKAMFVDFGLARE 300

Query: 301 RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG 360
           RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG
Sbjct: 301 RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG 360

Query: 361 QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD 420
           QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD
Sbjct: 361 QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD 420

Query: 421 NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASYY 480
           NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HCIVDSLENITHSLCTLEFETRRASYY
Sbjct: 421 NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCIVDSLENITHSLCTLEFETRRASYY 480

Query: 481 WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT 540
           WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT
Sbjct: 481 WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT 540

Query: 541 STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI 600
           STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI
Sbjct: 541 STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI 600

Query: 601 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 660
           MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI
Sbjct: 601 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 660

Query: 661 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 720
           KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE
Sbjct: 661 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 720

Query: 721 NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYF 765
           NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIG F
Sbjct: 721 NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGKF 766

BLAST of MC06g0001 vs. NCBI nr
Match: XP_008460942.1 (PREDICTED: glutamine--tRNA ligase-like [Cucumis melo])

HSP 1 Score: 1477 bits (3823), Expect = 0.0
Identity = 726/795 (91.32%), Postives = 761/795 (95.72%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT KN+ +DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVLDGC+RAVGNL
Sbjct: 1   MTAKNETIDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLDGCNRAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILL+YI SNK+KM AQLEAAFSF SA+GQED+KLNEFEDACGV
Sbjct: 61  LYTVATKYPTNALVHRPILLKYITSNKVKMPAQLEAAFSFFSAAGQEDIKLNEFEDACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEI+QTV EVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQFIDSKL
Sbjct: 121 GVEVSVEEIKQTVTEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQFIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK AKKKKEKP KV+ KT A +SPEQP SEEDLNPYL FPQP+DNYKV
Sbjct: 181 FELLGERTAADNEKIAKKKKEKPAKVEDKTAAIVSPEQPPSEEDLNPYLIFPQPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRC NTKELLEKHLKATGG+ LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCSNTKELLEKHLKATGGKVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIV+WMGWEPFKITY SDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVRWMGWEPFKITYASDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLF+EMKQGLI++GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFEEMKQGLIDEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLIRFDRLEYHIREELNR+A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIRFDRLEYHIREELNRTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADD ETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDNETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. NCBI nr
Match: XP_023550512.1 (glutamine--tRNA ligase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1476 bits (3820), Expect = 0.0
Identity = 725/795 (91.19%), Postives = 763/795 (95.97%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT+KN+N+DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVL+GC +AVGNL
Sbjct: 1   MTVKNENLDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLEGCDKAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILL+Y+ SNKIKM AQLEAAFSF SA+GQED+KLNEFED CGV
Sbjct: 61  LYTVATKYPTNALVHRPILLKYVTSNKIKMPAQLEAAFSFFSAAGQEDIKLNEFEDKCGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEIE+TVNEVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQ+IDSKL
Sbjct: 121 GVEVSVEEIEKTVNEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQYIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK  KKKKEKP KVD K VA +S EQP SEEDLNPYL FP+P+DNYKV
Sbjct: 181 FELLGERTAADNEKIVKKKKEKPAKVDDKPVAIVSVEQPPSEEDLNPYLIFPRPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGGR LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLI+F+RLEYHIREELN++A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIQFNRLEYHIREELNKTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADDKETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDKETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELD+WLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDNWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. NCBI nr
Match: KAG6579209.1 (hypothetical protein SDJN03_23657, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016722.1 hypothetical protein SDJN02_21832 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1476 bits (3820), Expect = 0.0
Identity = 724/795 (91.07%), Postives = 763/795 (95.97%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT+KN+N+DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVL+GC +A+GNL
Sbjct: 1   MTVKNENLDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLEGCDKAIGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILL+Y+ SNKIKM AQLEAAFSF SA+GQED+KLNEFED CGV
Sbjct: 61  LYTVATKYPTNALVHRPILLKYVTSNKIKMPAQLEAAFSFFSAAGQEDIKLNEFEDKCGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEIE+TVNEVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQ+IDSKL
Sbjct: 121 GVEVSVEEIEKTVNEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQYIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK  KKKKEKP KVD K VA +S EQP SEEDLNPYL FP+P+DNYKV
Sbjct: 181 FELLGERTAADNEKIVKKKKEKPAKVDDKPVAIVSVEQPPSEEDLNPYLIFPRPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGGR LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLI+F+RLEYHIREELN++A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIQFNRLEYHIREELNKTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIE+SDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIERSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADDKETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDKETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. NCBI nr
Match: XP_022994009.1 (glutamine--tRNA ligase [Cucurbita maxima])

HSP 1 Score: 1475 bits (3818), Expect = 0.0
Identity = 723/795 (90.94%), Postives = 763/795 (95.97%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT+KN+N+DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVL+GC +AVGNL
Sbjct: 1   MTVKNENLDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLEGCDKAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP+NALVHRPILL+Y+ SNKIKM AQLEAAFSF SA+GQED+KLNEFED CGV
Sbjct: 61  LYTVATKYPSNALVHRPILLKYVTSNKIKMPAQLEAAFSFFSAAGQEDIKLNEFEDKCGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEIE+TVNEVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQ+IDSKL
Sbjct: 121 GVEVSVEEIEKTVNEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQYIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK  KKKKEKP KVD K+VA +S EQP SEEDLNPYL FP+P+DNYKV
Sbjct: 181 FELLGERTAADNEKIVKKKKEKPAKVDDKSVAIVSVEQPPSEEDLNPYLIFPRPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGGR  TRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRVFTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLI+F+RLEYHIREELN++A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIQFNRLEYHIREELNKTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADDKETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDKETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNA+VGD FQFER+GYFAVD DSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAIVGDTFQFERLGYFAVDNDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. ExPASy TrEMBL
Match: A0A6J1BZM8 (Glutaminyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111006940 PE=3 SV=1)

HSP 1 Score: 1530 bits (3962), Expect = 0.0
Identity = 763/766 (99.61%), Postives = 763/766 (99.61%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL
Sbjct: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV
Sbjct: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL
Sbjct: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH 240
           YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH
Sbjct: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYKVH 240

Query: 241 TEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARE 300
           TEVFFSNGTILRCCNTKELLEKHLKATGGR LTRFPPEPNGYLHIGHAKAMFVDFGLARE
Sbjct: 241 TEVFFSNGTILRCCNTKELLEKHLKATGGRVLTRFPPEPNGYLHIGHAKAMFVDFGLARE 300

Query: 301 RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG 360
           RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG
Sbjct: 301 RGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIITG 360

Query: 361 QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD 420
           QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD
Sbjct: 361 QAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQSD 420

Query: 421 NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASYY 480
           NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HCIVDSLENITHSLCTLEFETRRASYY
Sbjct: 421 NFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCIVDSLENITHSLCTLEFETRRASYY 480

Query: 481 WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT 540
           WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT
Sbjct: 481 WLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVT 540

Query: 541 STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI 600
           STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI
Sbjct: 541 STAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGSI 600

Query: 601 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 660
           MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI
Sbjct: 601 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 660

Query: 661 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 720
           KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE
Sbjct: 661 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 720

Query: 721 NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYF 765
           NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIG F
Sbjct: 721 NPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGKF 766

BLAST of MC06g0001 vs. ExPASy TrEMBL
Match: A0A1S3CD39 (Glutaminyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103499677 PE=3 SV=1)

HSP 1 Score: 1477 bits (3823), Expect = 0.0
Identity = 726/795 (91.32%), Postives = 761/795 (95.72%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT KN+ +DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVLDGC+RAVGNL
Sbjct: 1   MTAKNETIDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLDGCNRAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILL+YI SNK+KM AQLEAAFSF SA+GQED+KLNEFEDACGV
Sbjct: 61  LYTVATKYPTNALVHRPILLKYITSNKVKMPAQLEAAFSFFSAAGQEDIKLNEFEDACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEI+QTV EVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQFIDSKL
Sbjct: 121 GVEVSVEEIKQTVTEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQFIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK AKKKKEKP KV+ KT A +SPEQP SEEDLNPYL FPQP+DNYKV
Sbjct: 181 FELLGERTAADNEKIAKKKKEKPAKVEDKTAAIVSPEQPPSEEDLNPYLIFPQPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRC NTKELLEKHLKATGG+ LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCSNTKELLEKHLKATGGKVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIV+WMGWEPFKITY SDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVRWMGWEPFKITYASDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLF+EMKQGLI++GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFEEMKQGLIDEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLIRFDRLEYHIREELNR+A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIRFDRLEYHIREELNRTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADD ETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDNETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. ExPASy TrEMBL
Match: A0A6J1K1Q6 (Glutaminyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111489830 PE=3 SV=1)

HSP 1 Score: 1475 bits (3818), Expect = 0.0
Identity = 723/795 (90.94%), Postives = 763/795 (95.97%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT+KN+N+DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVL+GC +AVGNL
Sbjct: 1   MTVKNENLDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLEGCDKAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP+NALVHRPILL+Y+ SNKIKM AQLEAAFSF SA+GQED+KLNEFED CGV
Sbjct: 61  LYTVATKYPSNALVHRPILLKYVTSNKIKMPAQLEAAFSFFSAAGQEDIKLNEFEDKCGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEIE+TVNEVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQ+IDSKL
Sbjct: 121 GVEVSVEEIEKTVNEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQYIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK  KKKKEKP KVD K+VA +S EQP SEEDLNPYL FP+P+DNYKV
Sbjct: 181 FELLGERTAADNEKIVKKKKEKPAKVDDKSVAIVSVEQPPSEEDLNPYLIFPRPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGGR  TRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRVFTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLI+F+RLEYHIREELN++A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIQFNRLEYHIREELNKTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADDKETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDKETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNA+VGD FQFER+GYFAVD DSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAIVGDTFQFERLGYFAVDNDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. ExPASy TrEMBL
Match: A0A0A0LIR0 (Glutaminyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_2G247610 PE=3 SV=1)

HSP 1 Score: 1471 bits (3809), Expect = 0.0
Identity = 724/795 (91.07%), Postives = 758/795 (95.35%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT KN+NVDKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVLDGC++ VGNL
Sbjct: 1   MTAKNENVDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLDGCNKTVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILLQYI SNK+KM AQLEAAFSF SA+GQED+KLN+FEDACGV
Sbjct: 61  LYTVATKYPTNALVHRPILLQYITSNKVKMPAQLEAAFSFFSAAGQEDIKLNDFEDACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVSVEEI+QTV EVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQFIDSKL
Sbjct: 121 GVEVSVEEIKQTVTEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQFIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK AKKKKEKP KV+ K  A +SPEQP SEEDLNPYL FPQP+DNYKV
Sbjct: 181 FELLGERTAADNEKIAKKKKEKPAKVEDKPAAIVSPEQPPSEEDLNPYLIFPQPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGG+ LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGKVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIV+WMGWEPFKITY SDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVRWMGWEPFKITYASDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQT +EIKEYREKKMNSPWRDRPVAESLKLF+EMKQGLI++GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTADEIKEYREKKMNSPWRDRPVAESLKLFEEMKQGLIDEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSN VLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNTVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELN+SA RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNKSAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKCTDVILADD ETVLE+RAEYDASKKSKPKGVLHWVA+PS GV+PL VEVRLFDKLFLS
Sbjct: 661 IKCTDVILADDNETVLEIRAEYDASKKSKPKGVLHWVAQPSSGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. ExPASy TrEMBL
Match: A0A6J1FG37 (Glutaminyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111445182 PE=3 SV=1)

HSP 1 Score: 1471 bits (3808), Expect = 0.0
Identity = 723/795 (90.94%), Postives = 760/795 (95.60%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           MT+KN+N+DKDKLLEQFLRIGLDERTARNT+ANNKVT+NLNAV+HEAGVL+GC +AVGNL
Sbjct: 1   MTVKNENLDKDKLLEQFLRIGLDERTARNTVANNKVTANLNAVIHEAGVLEGCDKAVGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LYTVATKYP NALVHRPILL+Y+ SNKIKM AQLEAAFSF SA+GQED+KLNEFED CGV
Sbjct: 61  LYTVATKYPTNALVHRPILLKYVTSNKIKMPAQLEAAFSFFSAAGQEDIKLNEFEDKCGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           G+EVS EEIE+TV EVFEEHK EILEQRYR NVGDLFGQVRKKHPWADPKIVKQ+IDSKL
Sbjct: 121 GVEVSAEEIEKTVYEVFEEHKNEILEQRYRTNVGDLFGQVRKKHPWADPKIVKQYIDSKL 180

Query: 181 YGLLGERTAADNEKTAKKKKEKPTKVDVKTVATISPEQP-SEEDLNPYLTFPQPQDNYKV 240
           + LLGERTAADNEK  KKKKEKP KVD K VA +S EQP SEEDLNPYL FP+P+DNYKV
Sbjct: 181 FELLGERTAADNEKIVKKKKEKPAKVDDKPVAIVSVEQPPSEEDLNPYLIFPRPEDNYKV 240

Query: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLAR 300
           HTEVFFSNGTILRCCNTKELLEKHLKATGGR LTRFPPEPNGYLHIGHAKAMFVDFGLA+
Sbjct: 241 HTEVFFSNGTILRCCNTKELLEKHLKATGGRVLTRFPPEPNGYLHIGHAKAMFVDFGLAK 300

Query: 301 ERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLIIT 360
           ERGG CYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAV+LI  
Sbjct: 301 ERGGGCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVELIRR 360

Query: 361 GQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQS 420
           G AYVDHQTP+EIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIE+GKATLRMKQDMQS
Sbjct: 361 GHAYVDHQTPDEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEEGKATLRMKQDMQS 420

Query: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRASY 480
           DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRASY
Sbjct: 421 DNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRASY 480

Query: 481 YWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540
           YWLLHAL+LYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV
Sbjct: 481 YWLLHALDLYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRGV 540

Query: 541 TSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENGS 600
           TSTAINAFVRG+GITRSDCSLI+F+RLEYHIREELN++A RAMVVLQPLKVVITNLENGS
Sbjct: 541 TSTAINAFVRGMGITRSDCSLIQFNRLEYHIREELNKTAARAMVVLQPLKVVITNLENGS 600

Query: 601 IMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYP 660
           I+DLDAKKWPEAQADEASAFYKVPFSN+VYIEQSDFRL DSKDYYGLAPGKSVLLRYAYP
Sbjct: 601 ILDLDAKKWPEAQADEASAFYKVPFSNIVYIEQSDFRLKDSKDYYGLAPGKSVLLRYAYP 660

Query: 661 IKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLS 720
           IKC DVILADDKETVLE+RAEYDASKKSKPKGVLHWVA+PSPGV+PL VEVRLFDKLFLS
Sbjct: 661 IKCADVILADDKETVLEIRAEYDASKKSKPKGVLHWVAQPSPGVNPLNVEVRLFDKLFLS 720

Query: 721 ENPAELDDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 780
           ENPAELDDWLADLNP SKVVIPSA+AVPELRNAVVGD FQFER+GYFAVDKDSTPEKL+F
Sbjct: 721 ENPAELDDWLADLNPHSKVVIPSAYAVPELRNAVVGDTFQFERLGYFAVDKDSTPEKLVF 780

Query: 781 NRTVTLRDGYNKSSK 793
           NRTVTLRD Y KSSK
Sbjct: 781 NRTVTLRDSY-KSSK 794

BLAST of MC06g0001 vs. TAIR 10
Match: AT1G25350.1 (glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative )

HSP 1 Score: 1253.4 bits (3242), Expect = 0.0e+00
Identity = 606/797 (76.04%), Postives = 691/797 (86.70%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           M +K+DN   +K +E F+ IGLDE+TARNTI NNKVT+NL AV+HEA V DGC R  GNL
Sbjct: 1   MVLKDDN--SEKSIELFISIGLDEKTARNTINNNKVTANLTAVIHEAAVTDGCDRNTGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LY+VATK+P NALVHRP LL+YI+++KIK  AQLEAAF+F +++G ED KLNEFE+ACGV
Sbjct: 61  LYSVATKFPTNALVHRPTLLKYIVNSKIKTPAQLEAAFAFFASTGPEDFKLNEFEEACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           GIEVS E+IE+ V  +FEE+KK ILEQRYR NVG+LFG VRK  PWADPKIVK+ ID K+
Sbjct: 121 GIEVSPEDIEKAVKGIFEENKKTILEQRYRTNVGELFGHVRKSLPWADPKIVKKLIDEKM 180

Query: 181 YGLLGERTAADNEKTAKK--KKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYK 240
           Y LLGE+TAADNEK  KK  KKEKP KV+ K     +  +PSEE+LNPY  FPQP+ N+ 
Sbjct: 181 YELLGEKTAADNEKPTKKKEKKEKPAKVEEKKAVVETTAEPSEEELNPYTIFPQPEQNFM 240

Query: 241 VHTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNGYLHIGHAKAMFVDFGLA 300
           VHTEVFFS+G+ILRC NTKE+L+KHLK TGG+  TRFPPEPNGYLHIGHAKAMFVDFGLA
Sbjct: 241 VHTEVFFSDGSILRCSNTKEVLDKHLKVTGGKVYTRFPPEPNGYLHIGHAKAMFVDFGLA 300

Query: 301 RERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELAVKLII 360
           +ERGG CYLRYDDTNPEAEK+EYI+HIEEIVKWMGWEPFKITYTSDYFQ+LY+LAV+LI 
Sbjct: 301 KERGGCCYLRYDDTNPEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLAVELIR 360

Query: 361 TGQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRMKQDMQ 420
            G AYVDHQT +EIKEYREKKMNSPWRDRP+ ESLKLFDEM++G+IE+GKATLRMKQDMQ
Sbjct: 361 RGHAYVDHQTADEIKEYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRMKQDMQ 420

Query: 421 SDNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFETRRAS 480
           SDNFNMYDLIAYRIKF PHP AGDKWCIYPSYDY HC VDSLENITHSLCTLEFETRRAS
Sbjct: 421 SDNFNMYDLIAYRIKFAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFETRRAS 480

Query: 481 YYWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAGLRRRG 540
           YYWLLH+L LY PYVWEYSRLNV+N V+SKRKLN +VT K+VDGWDDPRLLTL+GLRRRG
Sbjct: 481 YYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSGLRRRG 540

Query: 541 VTSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVITNLENG 600
           VTSTAINAFVRGIGITRSD S+I   RLE+HIREELN++A R MVVL PLKVVITNLE+ 
Sbjct: 541 VTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVITNLESD 600

Query: 601 SIMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAY 660
            +++LDAK+WP+AQ D+ SAFYKVPFS VVYI+QSDFR+ DSKDYYGLAPGKSVLLRYA+
Sbjct: 601 KLIELDAKRWPDAQNDDPSAFYKVPFSRVVYIDQSDFRMKDSKDYYGLAPGKSVLLRYAF 660

Query: 661 PIKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFL 720
           PIKCT+V+ ADD ETV E+ AEYD  KKSKPKGVLHWVAE SPG +P+KVEVRLF+KLF 
Sbjct: 661 PIKCTNVVFADDNETVREIHAEYDPEKKSKPKGVLHWVAESSPGEEPIKVEVRLFEKLFN 720

Query: 721 SENPAELDD-WLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKL 780
           SENPAEL+D WL D+NP SK+VI  A+AV  L++A VGD FQFER+GY+AVDKDS P KL
Sbjct: 721 SENPAELNDAWLTDINPNSKMVISGAYAVSTLKDAAVGDRFQFERLGYYAVDKDSEPGKL 780

Query: 781 IFNRTVTLRDGYNKSSK 794
           +FNRTVTLRD Y K  K
Sbjct: 781 VFNRTVTLRDSYGKGGK 795

BLAST of MC06g0001 vs. TAIR 10
Match: AT1G25350.2 (glutamine-tRNA ligase, putative / glutaminyl-tRNA synthetase, putative / GlnRS, putative )

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 599/802 (74.69%), Postives = 687/802 (85.66%), Query Frame = 0

Query: 1   MTMKNDNVDKDKLLEQFLRIGLDERTARNTIANNKVTSNLNAVVHEAGVLDGCSRAVGNL 60
           M +K+DN   +K +E F+ IGLDE+TARNTI NNKVT+NL AV+HEA V DGC R  GNL
Sbjct: 1   MVLKDDN--SEKSIELFISIGLDEKTARNTINNNKVTANLTAVIHEAAVTDGCDRNTGNL 60

Query: 61  LYTVATKYPANALVHRPILLQYIISNKIKMAAQLEAAFSFLSASGQEDVKLNEFEDACGV 120
           LY+VATK+P NALVHRP LL+YI+++KIK  AQLEAAF+F +++G ED KLNEFE+ACGV
Sbjct: 61  LYSVATKFPTNALVHRPTLLKYIVNSKIKTPAQLEAAFAFFASTGPEDFKLNEFEEACGV 120

Query: 121 GIEVSVEEIEQTVNEVFEEHKKEILEQRYRINVGDLFGQVRKKHPWADPKIVKQFIDSKL 180
           GIEVS E+IE+ V  +FEE+KK ILEQRYR NVG+LFG VRK  PWADPKIVK+ ID K+
Sbjct: 121 GIEVSPEDIEKAVKGIFEENKKTILEQRYRTNVGELFGHVRKSLPWADPKIVKKLIDEKM 180

Query: 181 YGLLGERTAADNEKTAKK--KKEKPTKVDVKTVATISPEQPSEEDLNPYLTFPQPQDNYK 240
           Y LLGE+TAADNEK  KK  KKEKP KV+ K     +  +PSEE+LNPY  FPQP+ N+ 
Sbjct: 181 YELLGEKTAADNEKPTKKKEKKEKPAKVEEKKAVVETTAEPSEEELNPYTIFPQPEQNFM 240

Query: 241 VHTEVFFSNGTILRCCNTKELLEKHLKATGGRGLTRFPPEPNG---YLHIG--HAKAMFV 300
           VHTEVFFS+G+ILRC NTKE+L+KHLK TGG+  TRFPPEPNG   Y+     + +AMFV
Sbjct: 241 VHTEVFFSDGSILRCSNTKEVLDKHLKVTGGKVYTRFPPEPNGLWFYVRFQCLYFQAMFV 300

Query: 301 DFGLARERGGSCYLRYDDTNPEAEKKEYIDHIEEIVKWMGWEPFKITYTSDYFQDLYELA 360
           DFGLA+ERGG CYLRYDDTNPEAEK+EYI+HIEEIVKWMGWEPFKITYTSDYFQ+LY+LA
Sbjct: 301 DFGLAKERGGCCYLRYDDTNPEAEKEEYINHIEEIVKWMGWEPFKITYTSDYFQELYDLA 360

Query: 361 VKLIITGQAYVDHQTPEEIKEYREKKMNSPWRDRPVAESLKLFDEMKQGLIEDGKATLRM 420
           V+LI  G AYVDHQT +EIKEYREKKMNSPWRDRP+ ESLKLFDEM++G+IE+GKATLRM
Sbjct: 361 VELIRRGHAYVDHQTADEIKEYREKKMNSPWRDRPIEESLKLFDEMRRGIIEEGKATLRM 420

Query: 421 KQDMQSDNFNMYDLIAYRIKFTPHPHAGDKWCIYPSYDY-HCIVDSLENITHSLCTLEFE 480
           KQDMQSDNFNMYDLIAYRIKF PHP AGDKWCIYPSYDY HC VDSLENITHSLCTLEFE
Sbjct: 421 KQDMQSDNFNMYDLIAYRIKFAPHPKAGDKWCIYPSYDYAHCTVDSLENITHSLCTLEFE 480

Query: 481 TRRASYYWLLHALELYQPYVWEYSRLNVSNNVLSKRKLNRLVTEKWVDGWDDPRLLTLAG 540
           TRRASYYWLLH+L LY PYVWEYSRLNV+N V+SKRKLN +VT K+VDGWDDPRLLTL+G
Sbjct: 481 TRRASYYWLLHSLSLYMPYVWEYSRLNVTNTVMSKRKLNYIVTNKYVDGWDDPRLLTLSG 540

Query: 541 LRRRGVTSTAINAFVRGIGITRSDCSLIRFDRLEYHIREELNRSAERAMVVLQPLKVVIT 600
           LRRRGVTSTAINAFVRGIGITRSD S+I   RLE+HIREELN++A R MVVL PLKVVIT
Sbjct: 541 LRRRGVTSTAINAFVRGIGITRSDGSMIHVSRLEHHIREELNKTAPRTMVVLNPLKVVIT 600

Query: 601 NLENGSIMDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVL 660
           NLE+  +++LDAK+WP+AQ D+ SAFYKVPFS VVYI+QSDFR+ DSKDYYGLAPGKSVL
Sbjct: 601 NLESDKLIELDAKRWPDAQNDDPSAFYKVPFSRVVYIDQSDFRMKDSKDYYGLAPGKSVL 660

Query: 661 LRYAYPIKCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLF 720
           LRYA+PIKCT+V+ ADD ETV E+ AEYD  KKSKPKGVLHWVAE SPG +P+KVEVRLF
Sbjct: 661 LRYAFPIKCTNVVFADDNETVREIHAEYDPEKKSKPKGVLHWVAESSPGEEPIKVEVRLF 720

Query: 721 DKLFLSENPAELDD-WLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDS 780
           +KLF SENPAEL+D WL D+NP SK+VI  A+AV  L++A VGD FQFER+GY+AVDKDS
Sbjct: 721 EKLFNSENPAELNDAWLTDINPNSKMVISGAYAVSTLKDAAVGDRFQFERLGYYAVDKDS 780

Query: 781 TPEKLIFNRTVTLRDGYNKSSK 794
            P KL+FNRTVTLRD Y K  K
Sbjct: 781 EPGKLVFNRTVTLRDSYGKGGK 800

BLAST of MC06g0001 vs. TAIR 10
Match: AT5G26710.1 (Glutamyl/glutaminyl-tRNA synthetase, class Ic )

HSP 1 Score: 240.0 bits (611), Expect = 6.7e-63
Identity = 168/503 (33.40%), Postives = 264/503 (52.49%), Query Frame = 0

Query: 269 GRGLTRFPPEPNGYLHIGHAKAMFVDFGLARERGGSCYLRYDDTNPEAEKKEYIDHIEEI 328
           G+   RF PEP+GYLHIGHAKA  ++   A    G   +R+DDTNP  E  E++D++ + 
Sbjct: 212 GKVKLRFAPEPSGYLHIGHAKAALLNKYFAERYQGEVIVRFDDTNPAKESNEFVDNLVKD 271

Query: 329 VKWMGWEPFKITYTSDYFQDLYELAVKLIITGQAYVDHQTPEEIKEYREKKMNSPWRDRP 388
           +  +G +  K+TYTSDYF +L ++A KL+  G+AYVD    E++++ R   ++S  R+  
Sbjct: 272 IGTLGIKYEKVTYTSDYFPELMDMAEKLMREGKAYVDDTPREQMQKERMDGIDSKCRNHS 331

Query: 389 VAESLKLFDEMKQGLIEDGKATLRMKQDMQSDNFNMYDLIAYRIKFTPHPHAGDKWCIYP 448
           V E+LKL+ EM  G     +  +R K +MQ  N  M D + YR     H   GDK+ IYP
Sbjct: 332 VEENLKLWKEMIAGSERGLQCCVRGKFNMQDPNKAMRDPVYYRCNPMSHHRIGDKYKIYP 391

Query: 449 SYDYHC-IVDSLENITHSLCTLEFETRRASYYWLLHALELYQPYVWEYSRLNVSNNVLSK 508
           +YD+ C  VDSLE ITH+L + E+  R A Y+ +L  + L Q  ++E+SRLN+   +LSK
Sbjct: 392 TYDFACPFVDSLEGITHALRSSEYHDRNAQYFKVLEDMGLRQVQLYEFSRLNLVFTLLSK 451

Query: 509 RKLNRLVTEKWVDGWDDPRLLTLAGLRRRGVTSTAINAFVRGIGITRSDCSLIRFDRLEY 568
           RKL   V    VDGWDDPR  T+ G+ RRG+   A+  F+   G ++ + +L+ +D+L  
Sbjct: 452 RKLLWFVQTGLVDGWDDPRFPTVQGIVRRGLKIEALIQFILEQGASK-NLNLMEWDKLWS 511

Query: 569 HIREELNRSAERAMVVLQPLKVVITNLENGSIMDLDAKKWPEAQADEASAFYKVPFSNVV 628
             +  ++    R   V+   +V+ T  +     +   +  P+ +  E +      F+  +
Sbjct: 512 INKRIIDPVCPRHTAVVAERRVLFTLTDGPD--EPFVRMIPKHKKFEGAGEKATTFTKSI 571

Query: 629 YIEQSDFRLNDSKDYYGLAPGKSV-LLRYAYPIKCTDVILADDKETVLEVRAEYDASKKS 688
           ++E++D           ++ G+ V L+ +   I        + + T L        S K+
Sbjct: 572 WLEEADAS--------AISVGEEVTLMDWGNAIVKEITKDEEGRVTALSGVLNLQGSVKT 631

Query: 689 KPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSENPAELDDWLAD-LNPQSKVVIPSAFAV 748
             K  L W+ + +  V+    E   FD L +++   E DD +AD +NP +K     A   
Sbjct: 632 -TKLKLTWLPDTNELVNLTLTE---FDYL-ITKKKLEDDDEVADFVNPNTKKE-TLALGD 691

Query: 749 PELRNAVVGDAFQFERIGYFAVD 769
             +RN   GD  Q ER GYF  D
Sbjct: 692 SNMRNLKCGDVIQLERKGYFRCD 697

BLAST of MC06g0001 vs. TAIR 10
Match: AT5G19720.1 (Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain )

HSP 1 Score: 234.6 bits (597), Expect = 2.8e-61
Identity = 116/195 (59.49%), Postives = 136/195 (69.74%), Query Frame = 0

Query: 600 MDLDAKKWPEAQADEASAFYKVPFSNVVYIEQSDFRLNDSKDYYGLAPGKSVLLRYAYPI 659
           M+LDAK+WP+A  D+ SAFYKVP S V+Y+EQSDF+  DSK+YYGLAPGKSVLLRY +PI
Sbjct: 1   MELDAKRWPDAPNDDPSAFYKVPLSRVIYMEQSDFQNEDSKNYYGLAPGKSVLLRYTFPI 60

Query: 660 KCTDVILADDKETVLEVRAEYDASKKSKPKGVLHWVAEPSPGVDPLKVEVRLFDKLFLSE 719
           KCT+V+ ADD +TV E+  EYD  KK KPKGVLHWV E SPG +P KVEV  F+ LF SE
Sbjct: 61  KCTNVVFADDTKTVCEIYVEYDPEKKIKPKGVLHWVPEYSPGKEPTKVEVCSFENLFNSE 120

Query: 720 NPAEL-DDWLADLNPQSKVVIPSAFAVPELRNAVVGDAFQFERIGYFAVDKDSTPEKLIF 779
           NPAEL DDWL D+NPQ                          + GY+ VDKDS P KL+F
Sbjct: 121 NPAELNDDWLTDINPQL-------------------------QSGYYTVDKDSIPGKLVF 170

Query: 780 NRTVTLRDGYNKSSK 794
           NRTVTL+DGY K  K
Sbjct: 181 NRTVTLKDGYKKGGK 170

BLAST of MC06g0001 vs. TAIR 10
Match: AT5G64050.1 (glutamate tRNA synthetase )

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-06
Identity = 33/122 (27.05%), Postives = 59/122 (48.36%), Query Frame = 0

Query: 268 GGRGLTRFPPEPNGYLHIGHAKAMFVDFGLARERGGSCYLRYDDTNPEAEKKEYIDHIEE 327
           GG    RF P P G LH+G A+    ++  AR +GG   LR +DT+ E   +E    + +
Sbjct: 51  GGSVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEAAVLQ 110

Query: 328 IVKWMG--WE----------PFKITYTSDYFQDLYELAVKLIITGQAYVDHQTPEEIKEY 378
            + W+G  W+          P++ +  +  ++   + A KL+ +G  Y    + EE+ + 
Sbjct: 111 DLSWLGLDWDEGPGVSGDFGPYRQSERNALYK---QYAEKLLESGHVYRCFCSSEELVKM 169