MC05g_new0356 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0356
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4-coumarate-CoA ligase
Location	MC05: 17941643 .. 17945909 (+)
RNA-Seq Expression	MC05g_new0356
Synteny	MC05g_new0356

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGATTCGAAATTAATATTTATTGCATTAACGTATAGATTCGAAATTAATCCATGGAATTTGGAGATTATTTTTCCACATAATTGACACGAGACGAAACTCCAACCTCATGATTTTGGGTATTCGTATTCTATAAACCGATTCTCTCTCATTTCCAAATCGAGTTACTGTATTTTATGATTATCTTCACAATCCTGTTATCTGGGAAAAACTCATTCGATTCTCGGGAAACAAACAAACCCCAGAAACGAAATCCGTCGATTTTCCCGTTCCTGAAGAATCCCGTTTTGCGTTTTTGTCTTTCCGGCGACAATGGCGATCAAGATGGGAAAATCTTTCAATCCCCAAACGAAGACGTACGCCTCTCCTCGGCCCCGCATCCACTTTCCGACGGACCCCACTCTCTCCATTGCCTCCTTCCTCTTCCGGTACTCATCATCTCGCCCCCACAGCCTCGCCCTCGCCGACGCCGATTCCGGCGAATCCCTCACTTTCCGGCAGCTTGAAATCCAAGTCTCCAAGCTAGCCCATGTGTTCGTCCAGCTCGGGATTCGGAAAAGCGATGTGGTTCTCATTTTCGCCCCCAATTCCATCCACTTCCCAGTTTGTTTCCTCGCTATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCCGCTTACACCTTCGCGGAATTGTCAAAGCAAGTCAAGGACTGTAACCCTAAGCTCGTAATCTCCGTCCCCGAACTCTGGAACGTGATCGGTAAACTGAAATTGCCGTCCATAATCCTCGGTTCTGAAATCTCTTCCAGTTTTTCGGGCTCCAATGTCTGGAGTTACTCCGATTTGATTGAGAAAGCCGGCGGACTAGTGTCCAGTTTGCCGGCCAGAGAAGTGGGCCAGAGCGATGTAGCGGCTCTTCTGTATTCATCTGGAACCACGGGGATCAGCAAGGGAGTGATTCTGACCCACAGGAACTTCATAACGACGTCGCTAATGGTGACGCATGATCAGGACCTTAGCGGTGATCCGAGCAACGTGTTTTTGTGCTTTCTCCCAATGTTCCATGTCTTCGGTCTCTCGATCATCGTTTACTCGCAGCTTCAGAGAGGGAACGCCGTCGTTTCAATGGCGAAGTTTGAGCTCGAAAGGGCTTTGGGGGTGGTGATGAAGTACAAGGTCACTCATCTCTATCTTGTCCCGCCGGTGCTTGTAGCTCTGACGAAGCAAGGTGTAGCAAAGAAGTATGATTTGTCGTCGTTGAAGCAAATTTTGGCCGGCGCGGCGCCTCTTGGGAAAGATGTGATGGAGGAATGCTCCAAGATTATCCCTCAGACCAAGATTACGCAGGTTTTGACCTGTGCTTCTCAATTCCATTTTTCATTTTCATCTGCTTCTTGCACAGGATGTATTATATTATCTTTAGATGATCTTTCTGAAGAATGTACATTATAATTGTTTGTTTGTCCGTATCTTTGTCTCTTGTTTCTTTATGCTACTTCCTAGAGTGTTGGGAGGAAACAGTTGAATTTGGGATGGGTCCCAATGACATAATCAAGCAAAAACTAAACTAATTTTTTTTGTGGTCTTGATTTGGAACTTGTGTTGTGTTCTACAGAAACTTGTGGAGTTATTTCAGTTGAGAATGTGGAAGAATCTAGGCTATCTGGATCAACTGGATTCCTTGTTTCAGGAGTTGAAGCCCAAATTTTGAGTGTAGAAACACAGAAACGTCTTCCACCTGGTGAAACAGGGGAAATATGTGTGCGTGGACCAAATATGATGCAAGGTGGATTTCTCCTTTGCTGTCTTGCTCTCTCCCTTATGCATTGCTTGGAGCGGATAGCTCGTAAAGCTTTATGTTATTAAACACCAAGCCCACTATTAAAAAACTCGTGAATTTGAGTATGGGAACAACTAAATTATGAATAGCTCATCCACACTCACTACTGTTGATTGGCCTAGTTGGTCAGTGAGGCTAGTGAAAAAATAGTAGGGTTCGGGGAGAATGGGTTAAAACTATGGTGGCCACTCTCGTAGGATTTTTGTTCGGTAGTTGTCCCATAAGATTAGGGCGTGCACAAGTTGACTTGGACACTCACGAGTATTTAAGGGAAAAAAATGAAAGAAAAAAGCTCATCCACACTAGTCATACTAAAAGGTGCACCAACCAGCCACCCTGAATTACACCTTGAACGATTTCCACATTGAAGCTTCTTATTGATTTTGCTTATTCTTTTATATTTGTTTTATTATGTTACTTAGCTGAACTTTCTTAACTGGATCTGCTTTTGAAAAAAAAAATTCCCCTCGGTTCCAAAGATGACAGTCTCATTTATTGATGATTTGTTCTTAGATTAATCAAGTTAGCACAAATGTGTTTCTTTTCCATTCTCATTTGCTAGTCTGCTTTGCAACATGTTCCTATGAACATTTCAGGATATTTCAACAATCAGAAAGCCAGCAGCCAAACTATTGATGATCAAGGATGGGTGCATACTGGAGATATCGGATATTTCAATGAAGGAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTCTGTTTTTCATAATCAAATATGCATTTTTTCTTCTTGGAGTTTTTGTTTCCATTTTAGGCGCTGTTTACAAGATCAAAATACAATTTTAACGAGTTATTGCTCCATCAGACTGCAACCAGTTCTTGTTCTATTCCCCCCTCTCATTCTTGTGTCATTTATATAGTTGTCTATAGAACTGCCGTCACCATCTTTTTTGACGAAATGAGTGCCTTTGTGTTGCTTTGCTTTTCTTTTTTTGATGTTTTGATGATGCTGTATGATTGGCTTTTTGAGTTCTTTCACGCTGTTTTTCTACCCAAAACTCCTAGATTTTAAGTTAATCCACATGGTGATGCAACTTGGATACTTTTTACACTGATCTATAATCTCTAATATCAAAAGAAACTGATTGAGTTTCTCTTCTCTGCCCAGATTTATCTTTAAGAATATGATCATCCATTTTATGATTGGCTTTGGAAAATATGTTTATTCTTATAATCGCATTCCCTCTTTTTCTTGGAAGTTTGAACTCTCAGAGCTGCATTTATTGATATCAAATTTTTGGGATTTTCTTGTATTAGGTTGCACCAGCAGAGCTTGAAGCGCTGCTACTGTCTCACCCTCAAATAATAGATGCCATTGTGATCCCGTAAGTAGATTATAGTAGCATCAGTAATGCCGTCATTACATGGACAGTTCATAGTGACTTTATTTTTCTATTTTCATGCTTAATAGTTAACCTCTGACATTAATTTCCACCTGCAGATATCCTGATGAAAAAGCTGGTGAGGTTCCCATCGCATTTGTTGTTCGTTCACCTAATAGTTCGATAAAGGAAGAAGATGTTCAAAATTTCATTGCAGGGCAGGTAATATTTTGAGAACTCTCAAACAAGAACAGAGGGAATGGAAGAGACTTCCTGTTGATATGGATCTATTATATCTCTTGAAAGCAATATTTCCAATTAAAATGTCATTGGTTGCATATACTCTTTACATGACTTGGGCCAATCTTTGTCTGATTCAAGAGGAAACCTTTGCACACCTTCGTTAACACTGAGACACTTAGTGTGCTGCACTAACCCGAAAAGGCAAAAGGTGACTGTTTTTAGCCACTAAAGTTGATTGGAAAATGCAAATTTTGGATTTGGAAGACAAAGTTTTCCCTCAATGTGCAAACAAGCATTTGGTGTCGAACTAAAACAGTCTCTTTCTAGATTGACTTGTTATTGATTCACTACACCCTGCATAAAAGTGGCAACCATACTTTTTTGACAATGGAATGCTTGAAAATGATCACTCTCTTTTCTCTTTGCAGGTCGCACCATACAAGAGATTAAAGAGAGTAACCTTTACAAGTAGTGTCCCGAAATCGGCTTCGGGAAAACTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAGATGCCAAAGCTGTTGAAAAGGTGAACAAGTCATACTATACCATATAGTTATAAAAAAATTAATTTAGAGCTACAAAATCCACAATCATGTAGCAAAAGGAAACCATGTAAAATGTTAAAATAAAACGACATACGATGCGATTCCAATAACATGAATCCAATTAAAGGATGACTAAAGCTCTGTAAGATTATAGGAACAATCTATGTACCAATGTGATAAAGATTCTGTTTTCATTATTCCCTTCTCTTCAACCACTACAACAAAGTAACTCCAATTTAGCTTTCTT

mRNA sequence

ATGGCGATCAAGATGGGAAAATCTTTCAATCCCCAAACGAAGACGTACGCCTCTCCTCGGCCCCGCATCCACTTTCCGACGGACCCCACTCTCTCCATTGCCTCCTTCCTCTTCCGGTACTCATCATCTCGCCCCCACAGCCTCGCCCTCGCCGACGCCGATTCCGGCGAATCCCTCACTTTCCGGCAGCTTGAAATCCAAGTCTCCAAGCTAGCCCATGTGTTCGTCCAGCTCGGGATTCGGAAAAGCGATGTGGTTCTCATTTTCGCCCCCAATTCCATCCACTTCCCAGTTTGTTTCCTCGCTATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCCGCTTACACCTTCGCGGAATTGTCAAAGCAAGTCAAGGACTGTAACCCTAAGCTCGTAATCTCCGTCCCCGAACTCTGGAACGTGATCGGTAAACTGAAATTGCCGTCCATAATCCTCGGTTCTGAAATCTCTTCCAGTTTTTCGGGCTCCAATGTCTGGAGTTACTCCGATTTGATTGAGAAAGCCGGCGGACTAGTGTCCAGTTTGCCGGCCAGAGAAGTGGGCCAGAGCGATGTAGCGGCTCTTCTGTATTCATCTGGAACCACGGGGATCAGCAAGGGAGTGATTCTGACCCACAGGAACTTCATAACGACGTCGCTAATGGTGACGCATGATCAGGACCTTAGCGGTGATCCGAGCAACGTGTTTTTGTGCTTTCTCCCAATGTTCCATGTCTTCGGTCTCTCGATCATCGTTTACTCGCAGCTTCAGAGAGGGAACGCCGTCGTTTCAATGGCGAAGTTTGAGCTCGAAAGGGCTTTGGGGGTGGTGATGAAGTACAAGGTCACTCATCTCTATCTTGTCCCGCCGGTGCTTGTAGCTCTGACGAAGCAAGGTGTAGCAAAGAAGTATGATTTGTCGTCGTTGAAGCAAATTTTGGCCGGCGCGGCGCCTCTTGGGAAAGATGTGATGGAGGAATGCTCCAAGATTATCCCTCAGACCAAGATTACGCAGGATATTTCAACAATCAGAAAGCCAGCAGCCAAACTATTGATGATCAAGGATGGGTGCATACTGGAGATATCGGATATTTCAATGAAGGAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTGCACCAGCAGAGCTTGAAGCGCTGCTACTGTCTCACCCTCAAATAA

Coding sequence (CDS)

ATGGCGATCAAGATGGGAAAATCTTTCAATCCCCAAACGAAGACGTACGCCTCTCCTCGGCCCCGCATCCACTTTCCGACGGACCCCACTCTCTCCATTGCCTCCTTCCTCTTCCGGTACTCATCATCTCGCCCCCACAGCCTCGCCCTCGCCGACGCCGATTCCGGCGAATCCCTCACTTTCCGGCAGCTTGAAATCCAAGTCTCCAAGCTAGCCCATGTGTTCGTCCAGCTCGGGATTCGGAAAAGCGATGTGGTTCTCATTTTCGCCCCCAATTCCATCCACTTCCCAGTTTGTTTCCTCGCTATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCCGCTTACACCTTCGCGGAATTGTCAAAGCAAGTCAAGGACTGTAACCCTAAGCTCGTAATCTCCGTCCCCGAACTCTGGAACGTGATCGGTAAACTGAAATTGCCGTCCATAATCCTCGGTTCTGAAATCTCTTCCAGTTTTTCGGGCTCCAATGTCTGGAGTTACTCCGATTTGATTGAGAAAGCCGGCGGACTAGTGTCCAGTTTGCCGGCCAGAGAAGTGGGCCAGAGCGATGTAGCGGCTCTTCTGTATTCATCTGGAACCACGGGGATCAGCAAGGGAGTGATTCTGACCCACAGGAACTTCATAACGACGTCGCTAATGGTGACGCATGATCAGGACCTTAGCGGTGATCCGAGCAACGTGTTTTTGTGCTTTCTCCCAATGTTCCATGTCTTCGGTCTCTCGATCATCGTTTACTCGCAGCTTCAGAGAGGGAACGCCGTCGTTTCAATGGCGAAGTTTGAGCTCGAAAGGGCTTTGGGGGTGGTGATGAAGTACAAGGTCACTCATCTCTATCTTGTCCCGCCGGTGCTTGTAGCTCTGACGAAGCAAGGTGTAGCAAAGAAGTATGATTTGTCGTCGTTGAAGCAAATTTTGGCCGGCGCGGCGCCTCTTGGGAAAGATGTGATGGAGGAATGCTCCAAGATTATCCCTCAGACCAAGATTACGCAGGATATTTCAACAATCAGAAAGCCAGCAGCCAAACTATTGATGATCAAGGATGGGTGCATACTGGAGATATCGGATATTTCAATGAAGGAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTTTCAGGTTGCACCAGCAGAGCTTGAAGCGCTGCTACTGTCTCACCCTCAAATAA

Protein sequence

MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGLVSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCFLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQGVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQDISTIRKPAAKLLMIKDGCILEISDISMKEENYLLWTESKNSSSVMVFRLHQQSLKRCYCLTLK

Homology

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BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 8.0e-118
Identity = 219/338 (64.79%), Postives = 266/338 (78.70%), Query Frame = 0


Query: 5   MGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQL 64
           M K FNP+T+ Y+S RP ++FPTDP LS+ SFLFR S+S P+  AL DADSG++LTF +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 65  EIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSK 124
           + QVSKLAH  VQL I+K+DVVLIFAPNSIHFPVCF +I A+GAI TTCNP+YTF ELS 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 125 QVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSG-SNVWSYSDLIEKAGGLVSS 184
           Q KDCNP LVI+VPELW    KL LP+IIL S  +S  S  S  W +SDL  K+    S 
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRF-SE 180

Query: 185 LPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCFLP 244
           LP  +V QSDVAALLYSSGTTG SKGV+L+H+NFITTSLMVT DQD  GDP N+ +CFLP
Sbjct: 181 LPISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLP 240

Query: 245 MFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQG-V 304
           MFH+FGLS+I YSQL+RGN VVSM KFELE AL  V  Y+VTHL++VPPV++AL K+  V
Sbjct: 241 MFHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVV 300

Query: 305 AKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
            ++YDLSS+K+IL+GAAPLGK+VME+C++ +P   I Q
Sbjct: 301 VRRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQ 337

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BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.7e-102
Identity = 200/339 (59.00%), Postives = 255/339 (75.22%), Query Frame = 0


Query: 5   MGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQL 64
           M KS   +   + S RP +  P D  LS+ SF+FR SSS P   AL D+D+ E+L+F Q 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 65  EIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSK 124
           +  V K++H F+ LG++K+DVVLIFAPNSIH PVCFL IVA GAIATT NP YT +ELSK
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 125 QVKDCNPKLVISVPELWNVIGKLKLPSIILG--SEISSSF-SGSNVWSYSDLIEKAGGLV 184
           QVKD NPKL+++VPEL+  +    LP+I++G  SE SS   S + V ++ DL+  +G  V
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGP-V 180

Query: 185 SSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCF 244
           S  P  +  QSD AALLYSSGTTG+SKGV+L+H+NFI +SLMVT +QD +G+  NVFLCF
Sbjct: 181 SDFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCF 240

Query: 245 LPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQG 304
           LPMFHVFGL+II Y+QLQRGN V+SMA+F+LE+ L  V KYKVTHL++VPPV++ALTK  
Sbjct: 241 LPMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNS 300

Query: 305 VAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           + KKYDLSSLK I +GAAPLGKD+MEEC+KI+P   + Q
Sbjct: 301 IVKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQ 338

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BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 4.4e-100
Identity = 198/339 (58.41%), Postives = 249/339 (73.45%), Query Frame = 0


Query: 5   MGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQL 64
           M KS   +   Y S RP +  P DP  S+ SFLFR SSS P  LA+AD+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  EIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSK 124
           +  V++LAH F +LGIRK+DVVLIFAPNS  FP+CFLA+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSF---SGSNVWSYSDLIEKAGGLV 184
           Q+KD NPK++ISV +L++ I    LP ++LGS+ +      S S + S+ +++E +   V
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEP-V 180

Query: 185 SSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCF 244
           S  P  E+ QSD AALLYSSGTTG SKGV LTH NFI  SLMVT DQDL G+   VFLCF
Sbjct: 181 SEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCF 240

Query: 245 LPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQG 304
           LPMFHVFGL++I YSQLQRGNA+VSMA+FELE  L  + K++VTHL++VPPV +AL+KQ 
Sbjct: 241 LPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQS 300

Query: 305 VAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           + KK+DLSSLK I +GAAPLGKD+MEEC + IP   + Q
Sbjct: 301 IVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQ 338

[Close]

BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 7.6e-76
Identity = 161/331 (48.64%), Postives = 219/331 (66.16%), Query Frame = 0


Query: 16  YASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADS-GESLTFRQLEIQV-SKLAH 75
           Y S RP     +DP LS+   L R + + P ++ALADA + G +LTF +L   V S    
Sbjct: 17  YRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRSAVLSTAVA 76

Query: 76  VFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSKQVKDCNPKL 135
           +  + G+R  D VL+ APN + +PVCF A+ A+GA+ TT NP YT  E++KQV D   KL
Sbjct: 77  LSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAKL 136

Query: 136 VISVPELWNVIGKLKLPSIILGSEISSSFSG----SNVWSYSDLIEKAGGLVSSLPAREV 195
           VI++  L   I  L+LP I+L  + +++ +     + V  Y++L+  AG   +      +
Sbjct: 137 VITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLV--AGVKEADYRRPPI 196

Query: 196 GQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCFLPMFHVFG 255
            QSD AALLYSSGTTG SKGVILTHRNFI  + MVT DQD   +  NVFLCFLPMFH+FG
Sbjct: 197 KQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHIFG 256

Query: 256 LSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQGVAKKYDLS 315
           LS+I Y+QL RGNA+++M++F++   +  V +++VTHL+ VPPV++AL K G A KYDLS
Sbjct: 257 LSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYDLS 316

Query: 316 SLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           SLK I +GAAPLGKDVME  +K  P ++I Q
Sbjct: 317 SLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQ 345

[Close]

BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.8e-54
Identity = 126/334 (37.72%), Postives = 192/334 (57.49%), Query Frame = 0


Query: 16  YASPRPRIHFPTDPTLSIASFLFRYSSSRPH--SLALADADSGESLTFRQLEIQVSKLAH 75
           + S R  +  P + ++ + +F+    SSR H   +A  DA +G  LTF QL   V  +A 
Sbjct: 22  FYSKREPVQLPQNHSVDVTTFI----SSRAHHGKIAFIDAATGRHLTFPQLWRAVDSVAT 81

Query: 76  VFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSKQVKDCNPKL 135
               +GIRK DV+L+ +PNSI+FPV  LA++++GAI TT NP  T  E++KQ+ D  P L
Sbjct: 82  CLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPVL 141

Query: 136 VISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGLVSSLPA-----RE 195
             ++P+L + I    LP +I+  E+ SS   +      +++   G ++   P+       
Sbjct: 142 AFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKT-----LNIVSSLGEMMRKEPSPNRIGYR 201

Query: 196 VGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCFLPMFHVF 255
           V Q D A LLYSSGTTG SKGV+ +H+N I   +     +  + D  + F+C +PMFH++
Sbjct: 202 VNQEDTATLLYSSGTTGASKGVVSSHKNLI-AMVQTILSRFGTEDGEHTFICTVPMFHIY 261

Query: 256 GLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQG--VAKKY 315
           GL+      L  G+ +V ++KFE+   L  + KY+ T+L LVPP+L+AL K    +  KY
Sbjct: 262 GLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAKY 321

Query: 316 DLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           DLSSL+ +L+G APL K+V+E   +  P   I Q
Sbjct: 322 DLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQ 345

[Close]

BLAST of MC05g_new0356 vs. NCBI nr
Match: XP_022982901.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 556 bits (1433), Expect = 2.00e-193
Identity = 281/340 (82.65%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI M KSFNPQ++ Y SPRP IHFPTDPT+SI SFLFR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           E+SKQV  CNPKLVI++PELW+VIGKL LPSIILGS+ISS  S  N+W YSDLI+ +G  
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS LP  EVGQSDVAALLYSSGTTGI+KGVILTHRNFITTSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K+YDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. NCBI nr
Match: XP_022934454.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 554 bits (1427), Expect = 1.63e-192
Identity = 281/340 (82.65%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI M KSF+PQ++ Y SPRP IHFPTDPT+SI SF+FR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPSIILGSEISS  S  N+W YSDLI+ +G  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS  P  EVGQSDVAALLYSSGTTGISKGVILTHRNFI TSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V KKYDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. NCBI nr
Match: KAG7017376.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555 bits (1430), Expect = 1.95e-192
Identity = 282/340 (82.94%), Postives = 306/340 (90.00%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI M KSF+PQ++ Y SPRP IHFPTDPT+SI SFLFR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPSIILGS+ISS  S  N+W YSDLI+ +G  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS LP  EVGQSDVAALLYSSGTTGISKGVILTHRNFI TSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V KKYDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. NCBI nr
Match: XP_023526195.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553 bits (1425), Expect = 3.51e-192
Identity = 279/340 (82.06%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           +AI M KSFNPQ++ Y SPRP IHFPTDPT+SI SFLFR SSS  H+LAL DADSGESLT
Sbjct: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVS+LAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTFA
Sbjct: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPS+ILGS+ISS  S  N+W YSDLI+ +G  
Sbjct: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPSVILGSKISSESSRPNIWVYSDLIKNSGD- 182

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS LP  EVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDL GDP NVFLC
Sbjct: 183 VSDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLC 242

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 243 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 302

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K+YDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 303 SVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 341

[Close]

BLAST of MC05g_new0356 vs. NCBI nr
Match: KAG6580619.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551 bits (1420), Expect = 1.88e-191
Identity = 280/340 (82.35%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI + K F+PQ++ Y SPRP IHFPTDPT+SI SFLFR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAVAKYFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPSIILGS+ISS  S  N+W YSDLI+ +G  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS LP  EVGQSDVAALLYSSGTTGISKGVILTHRNFI TSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V KKYDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL
Match: A0A6J1J0P0 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 9.69e-194
Identity = 281/340 (82.65%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI M KSFNPQ++ Y SPRP IHFPTDPT+SI SFLFR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           E+SKQV  CNPKLVI++PELW+VIGKL LPSIILGS+ISS  S  N+W YSDLI+ +G  
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS LP  EVGQSDVAALLYSSGTTGI+KGVILTHRNFITTSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K+YDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL
Match: A0A6J1F2M7 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=4 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 7.89e-193
Identity = 281/340 (82.65%), Postives = 305/340 (89.71%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           MAI M KSF+PQ++ Y SPRP IHFPTDPT+SI SF+FR SSS  HSLALADADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAH F+ LGIRK DVVLIFAPNSIHFPVCF AIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPSIILGSEISS  S  N+W YSDLI+ +G  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS  P  EVGQSDVAALLYSSGTTGISKGVILTHRNFI TSLMVTHDQDL GDP NVFLC
Sbjct: 181 VSDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+I+YSQLQRGN VVSMAKFELE+ALGVVMKY++THLYLVPPV++A+ KQ
Sbjct: 241 FLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V KKYDLSSLKQIL+GAAPLGKDVMEECSK++PQTKITQ
Sbjct: 301 SVVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ 339

[Close]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL
Match: A0A5A7TKZ4 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002730 PE=4 SV=1)

HSP 1 Score: 549 bits (1414), Expect = 7.41e-191
Identity = 281/340 (82.65%), Postives = 309/340 (90.88%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           M I M KSFNPQ++ Y+SPRP IHFPTDP +SI SFLFR SSS P++LALADADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLA VF QLGI+K DVVLIFAPNSIHF VCF AIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPS+ILGS+ISS FS SN+WSYSDLI+KAG  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS+LP  EVGQ+DVAALLYSSGTTGISKGVILTHRNFITTSLMVT DQ+L GDP NVFLC
Sbjct: 181 VSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+IVYSQLQRGN VVSMAKFELE+ALG+VMKYK+THLY+VPPV++ALTKQ
Sbjct: 241 FLPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K YDLSSL+QIL+GAAPLGKDVMEECSKIIPQ +I Q
Sbjct: 301 KVVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQ 339

[Close]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL
Match: A0A1S3B5U8 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1)

HSP 1 Score: 549 bits (1414), Expect = 7.41e-191
Identity = 281/340 (82.65%), Postives = 309/340 (90.88%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           M I M KSFNPQ++ Y+SPRP IHFPTDP +SI SFLFR SSS P++LALADADSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLA VF QLGI+K DVVLIFAPNSIHF VCF AIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +CNPKLVI+VPELW+VIGKL LPS+ILGS+ISS FS SN+WSYSDLI+KAG  
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS+LP  EVGQ+DVAALLYSSGTTGISKGVILTHRNFITTSLMVT DQ+L GDP NVFLC
Sbjct: 181 VSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLS+IVYSQLQRGN VVSMAKFELE+ALG+VMKYK+THLY+VPPV++ALTKQ
Sbjct: 241 FLPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K YDLSSL+QIL+GAAPLGKDVMEECSKIIPQ +I Q
Sbjct: 301 KVVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQ 339

[Close]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL
Match: A0A0A0LDK0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1)

HSP 1 Score: 537 bits (1383), Expect = 3.74e-186
Identity = 275/340 (80.88%), Postives = 304/340 (89.41%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLT 60
           M I M KSFNPQ++ Y S RP IHFPTDPT+SI SFLFR SSS P++LAL DADSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFA 120
           FRQL+IQVSKLAHVF+QLGI+K DVVLIF+PNSIHF VCF AIVAIGAIATTCNPAYT A
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFSPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSFSGSNVWSYSDLIEKAGGL 180
           ELSKQV +C PKLVI+VPELW+VIGKL LPSIILGS+ISS FS SN+W YSDLI+KAG  
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWGYSDLIKKAGD- 180

Query: 181 VSSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLC 240
           VS+LP  EVGQ+DVAALLYSSGTTGISKGVILTHRNFIT SLMVT DQ+L GDP NVFLC
Sbjct: 181 VSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLC 240

Query: 241 FLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQ 300
           FLPMFHVFGLSI+V SQLQRGN VVSMAKFELE+ALG+VMKYK+THLY+VPPV++ALTKQ
Sbjct: 241 FLPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQ 300

Query: 301 GVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 340
            V K YDLSSL+QIL+GAAPLGKDVM+ECSKIIPQ +I Q
Sbjct: 301 KVVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQ 339

[Close]

BLAST of MC05g_new0356 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 366.3 bits (939), Expect = 3.1e-101
Identity = 198/339 (58.41%), Postives = 249/339 (73.45%), Query Frame = 0


Query: 5   MGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQL 64
           M KS   +   Y S RP +  P DP  S+ SFLFR SSS P  LA+AD+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  EIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSK 124
           +  V++LAH F +LGIRK+DVVLIFAPNS  FP+CFLA+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVKDCNPKLVISVPELWNVIGKLKLPSIILGSEISSSF---SGSNVWSYSDLIEKAGGLV 184
           Q+KD NPK++ISV +L++ I    LP ++LGS+ +      S S + S+ +++E +   V
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEP-V 180

Query: 185 SSLPAREVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSNVFLCF 244
           S  P  E+ QSD AALLYSSGTTG SKGV LTH NFI  SLMVT DQDL G+   VFLCF
Sbjct: 181 SEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCF 240

Query: 245 LPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTKQG 304
           LPMFHVFGL++I YSQLQRGNA+VSMA+FELE  L  + K++VTHL++VPPV +AL+KQ 
Sbjct: 241 LPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQS 300

Query: 305 VAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           + KK+DLSSLK I +GAAPLGKD+MEEC + IP   + Q
Sbjct: 301 IVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQ 338

[Close]

BLAST of MC05g_new0356 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 204.5 bits (519), Expect = 1.6e-52
Identity = 131/346 (37.86%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 2   AIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPH--SLALADADSGESL 61
           ++     F     T+ S R  I  P +P+L + +F+    SS+ H   +A  DA +G++L
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI----SSQAHRGRIAFIDASTGQNL 62

Query: 62  TFRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTF 121
           TF +L   V  +A    ++GIRK  VVL+ +PNSI FPV  L+++++GAI TT NP  T 
Sbjct: 63  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 122

Query: 122 AELSKQVKDCNPKLVISVPELWNVIGKL--KLPSIILGSEISSSFSGSNVWSYSDLIEKA 181
            E++KQ+KD NP L  +  +L   I     KLP +++  E   S     V     L+E  
Sbjct: 123 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDS-----VGDVRRLVEMM 182

Query: 182 GGLVSSLPARE-VGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSN 241
               S    +E V Q D A LLYSSGTTG+SKGVI +HRN I     +  ++  S D   
Sbjct: 183 KKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIV-NRFGSDDGEQ 242

Query: 242 VFLCFLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVA 301
            F+C +PMFH++GL+      L  G+ ++ ++KFE+   +  + KY+ T L LVPP+LVA
Sbjct: 243 RFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVA 302

Query: 302 LTK--QGVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           +      +  KYDLSS+  +L G APL K+V E  ++  P  KI Q
Sbjct: 303 MVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQ 338

[Close]

BLAST of MC05g_new0356 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 204.5 bits (519), Expect = 1.6e-52
Identity = 131/346 (37.86%), Postives = 192/346 (55.49%), Query Frame = 0


Query: 2   AIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPH--SLALADADSGESL 61
           ++     F     T+ S R  I  P +P+L + +F+    SS+ H   +A  DA +G++L
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI----SSQAHRGRIAFIDASTGQNL 62

Query: 62  TFRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTF 121
           TF +L   V  +A    ++GIRK  VVL+ +PNSI FPV  L+++++GAI TT NP  T 
Sbjct: 63  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 122

Query: 122 AELSKQVKDCNPKLVISVPELWNVIGKL--KLPSIILGSEISSSFSGSNVWSYSDLIEKA 181
            E++KQ+KD NP L  +  +L   I     KLP +++  E   S     V     L+E  
Sbjct: 123 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDS-----VGDVRRLVEMM 182

Query: 182 GGLVSSLPARE-VGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLSGDPSN 241
               S    +E V Q D A LLYSSGTTG+SKGVI +HRN I     +  ++  S D   
Sbjct: 183 KKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIV-NRFGSDDGEQ 242

Query: 242 VFLCFLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVA 301
            F+C +PMFH++GL+      L  G+ ++ ++KFE+   +  + KY+ T L LVPP+LVA
Sbjct: 243 RFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVA 302

Query: 302 LTK--QGVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           +      +  KYDLSS+  +L G APL K+V E  ++  P  KI Q
Sbjct: 303 MVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQ 338

[Close]

BLAST of MC05g_new0356 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-50
Identity = 127/339 (37.46%), Postives = 193/339 (56.93%), Query Frame = 0


Query: 9   FNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPHSLALADADSGESLTFRQLEIQV 68
           F+ +T  Y S  P +H P DP L   S LF  S       AL D+ +G S++  +L+I V
Sbjct: 25  FSSKTGIYTSKFPSLHLPVDPNLDAVSALF--SHKHHGDTALIDSLTGFSISHTELQIMV 84

Query: 69  -SKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYTFAELSKQVK 128
            S  A ++  LG+R+ DVV +  PNS++FP+ FL+++++GAI TT NP+ +  E+ KQV 
Sbjct: 85  QSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVS 144

Query: 129 DCNPKLVISVPELWNVIGKLKLPSIILGSEIS-SSFSGSNVWSYSDLIEKAGGLVSSLPA 188
           +C+  L  +  E    +  L +  I +       S    N   YS + E  G     +P 
Sbjct: 145 ECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFG----FVPK 204

Query: 189 REVGQSDVAALLYSSGTTGISKGVILTHRNFITT-SLMVTHDQDLSGDP--SNVFLCFLP 248
             + Q DVAA++YSSGTTG SKGV+LTHRN I +  L V  +      P  SNV+L  LP
Sbjct: 205 PLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALP 264

Query: 249 MFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHLYLVPPVLVALTK--QG 308
           + H++GLS+ V   L  G+ +V M +F+    + V+ ++K+TH  +VPP+L+ALTK  +G
Sbjct: 265 LCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKG 324

Query: 309 VAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
           V  +    SLKQ+ +GAAPL +  +E+  + +P   + Q
Sbjct: 325 VCGEV-FKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQ 356

[Close]

BLAST of MC05g_new0356 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.6e-44
Identity = 119/355 (33.52%), Postives = 188/355 (52.96%), Query Frame = 0


Query: 1   MAIKMGKSFNPQTKTYASPRPRIHFPTDPTLSIASFLFRYSSSRPH--SLALADADSGES 60
           +A+     F   T  + S R  +  P +  L + SF+    +S+PH       DA +G  
Sbjct: 19  LAVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFI----ASQPHRGKTVFVDAVTGRR 78

Query: 61  LTFRQLEIQVSKLAHVFVQLGIRKSDVVLIFAPNSIHFPVCFLAIVAIGAIATTCNPAYT 120
           L+F +L + V ++A     LG+RK +VV+I +PNSI FP+  L+++++GAI TT NP  T
Sbjct: 79  LSFPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINT 138

Query: 121 FAELSKQVKDCNPKLVISVPELWNVI---GKLKLPSIILGSEISSSFSGSNVWSYSDLIE 180
             E+SKQ+ D  P L  +  +L + +       LP +++      S       SY D ++
Sbjct: 139 SDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPS------QSYGDRVK 198

Query: 181 KAGGLVSSLPA--------REVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHD 240
             G L + +          + V Q D AALLYSSGTTG SKGV+L+HRN I  +L+  + 
Sbjct: 199 LVGRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLI--ALVQAYR 258

Query: 241 QDLSGDPSNVFLCFLPMFHVFGLSIIVYSQLQRGNAVVSMAKFELERALGVVMKYKVTHL 300
                +   +  C +PM H+FG        +  G  +V + KF++ + L  V  ++ ++L
Sbjct: 259 ARFGLEQRTI--CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYL 318

Query: 301 YLVPPVLVALTK--QGVAKKYDLSSLKQILAGAAPLGKDVMEECSKIIPQTKITQ 341
            LVPP++VA+      +  KYDLSSL  ++AG APL ++V E+  +  P+ KI Q
Sbjct: 319 SLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQ 359

The following BLAST results are available for this feature:

BLAST of MC05g_new0356 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQS1	8.0e-118	64.79	Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1	[more]
M4IRL6	4.7e-102	59.00	Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1	[more]
Q9M0X9	4.4e-100	58.41	4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=...	[more]
Q0DV32	7.6e-76	48.64	4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1...	[more]
M4IQR7	4.8e-54	37.72	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]

BLAST of MC05g_new0356 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982901.1	2.00e-193	82.65	4-coumarate--CoA ligase-like 7 [Cucurbita maxima]	[more]
XP_022934454.1	1.63e-192	82.65	4-coumarate--CoA ligase-like 7 [Cucurbita moschata]	[more]
KAG7017376.1	1.95e-192	82.94	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023526195.1	3.51e-192	82.06	4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo]	[more]
KAG6580619.1	1.88e-191	82.35	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC05g_new0356 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J0P0	9.69e-194	82.65	4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 ...	[more]
A0A6J1F2M7	7.89e-193	82.65	4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=...	[more]
A0A5A7TKZ4	7.41e-191	82.65	4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B5U8	7.41e-191	82.65	4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1	[more]
A0A0A0LDK0	3.74e-186	80.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1	[more]

BLAST of MC05g_new0356 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05160.1	3.1e-101	58.41	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	1.6e-52	37.86	OPC-8:0 CoA ligase1	[more]
AT1G20510.2	1.6e-52	37.86	OPC-8:0 CoA ligase1	[more]
AT4G19010.1	1.1e-50	37.46	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	1.6e-44	33.52	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 42..337 e-value: 6.6E-59 score: 199.6
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 15..342 e-value: 5.3E-80 score: 271.2
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 8..346
None	No IPR available	PANTHER	PTHR24096:SF373	4-COUMARATE:COA LIGASE-LIKE PROTEIN	coord: 8..346
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 16..335
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 197..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0356.1	MC05g_new0356.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016405	CoA-ligase activity