MC05g_new0320 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g_new0320
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNADH-ubiquinone oxidoreductase chain 5
LocationMC05: 16235158 .. 16236762 (-)
RNA-Seq ExpressionMC05g_new0320
SyntenyMC05g_new0320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGAAGTCCATTACTGAGAAAAGTGGTGATCTCGTCTTGCTTGCTGGGAGAGAGGAAGCTTCCGGACCACCTTTTCCAGCCAGATAGCGAGCGAAACGGCCTCGCTAACGCGCCCCTTATTGAAAACTCAAGGAAAGCCTGGTAGGAATTCCTCAGGGCGTATTACGGTTTTTCACCGAGGGGGTGGATCGAAGCGATTGCATGCATCCGGTAAGCTCTCCGTTATATGCATGCTAATGGGGCAAGTATGTTTCTTCCCTGGATGAAATCCTATCTGTCCAGTCAGTCAATCAGGAGGTGGTCGACTCATCACATCCTGGGGTTGAAGAAGGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGGTACGTGAGTTGGGTTTAGAACGTCGTTCGACTTCCTAGCCTGCCTGGTCTACGCCCATCAAAATGGAGCAGTCAGGTTTGTCTACGAGGAACTTCAAAGAGCAGCCACACTTGGAGCCTACGACGAAGCACTGCGTAGGTTTCTTAAGGCAGGGATGCTTCTGGAGCTAAAAGGAAAAAAAGAGAATTGATTTCACAGCTCCTCTTTGCCCCCTCTTTTCTCAAGAATGAAAAAAAAAGATCCTGTTCTTTGAGAAAAGAAGACAAAAGCTCTTTTTTCTGCAACACTGCCTCTGTTTGAGAAGCCCGCGAAAGCTCGCTCGACTTAATAATAGCTGTCTTACTCGACTTGCTTACTAGCTGCCTTTCCATGGTGGATCAGGACGAGAATGAATTCGGGAATCCAGGACATACTCATCCTGGAGCTTCTGCTCTTTTCTGGGGGGGCTAGCTAGCTAGGCTAGCGCGCAATGGCTTTCTCTGCGTTGGGCTCGCTTCTTCAAGCCGGAGCCCAACCTATACAAGACAAGGTGCTGCTCGCTCAGGAAGTGGATATGCTGTTAGCTCTACCGGAGATAGGTATGGAAGAGCAGAGGACTGAAAATCCTCGTGTCACCAGTTCAAATCTGGTTCTTGGCACATCATGAATTTGCGTCTGCATCCGCATCAGTCGAAGTGGTGGAACGCCCTCCTGAGTCAAAGAGAGAGCTTCTGCACCTCTCTCCAAGAGATACGATCGGTATCAGAATCGGCTGAAGGAGAGTGAAGAGGCGCTACTCTGCTTATACAGTCAATCTTTACACACATGATAGTGGCAGCCAGGTTATCGACGATTCTACAATAGGCGGCCGCTACCCGACGAGTCTTCTTCAGTCCTGCAAGTACTGCTGGACTCGTTTCGAGCGTGAGTCTCTACTGCTCCGAAAACTAAGGATGAAGAGAGGTAGACAGGAAATGGGGTCTTACAGAGTCCTCTAGTTCTGGCCCGGTGTTTTTCGAGAAAAGGTCCGATCCACTCTTCGATCTTCTCTTTTTTTGCTTTTTATGAAGTCGCACTGGGAGCTAGTGCTTGCTATCTAAGAATTGCTCCATGGATCTCATCGGAAATGTTTGATGCTTCTTGGGGCTTCTTTGGCGACCGTGAAGTCACCGGCTTCGTGACCCGAACACGATTGGTGCTGACTGACGGGACAGATAGGATTTCATCTAGGGATAGACAGAAGCAACTGACATAG

mRNA sequence

ATGAAAGGAAGTCCATTACTGAGAAAAGTGGTGATCTCGTCTTGCTTGCTGGGAGAGAGGAAGCTTCCGGACCACCTTTTCCAGCCAGATAGCGAGCGAAACGGCCTCGCTAACGCGCCCCTTATTGAAAACTCAAGGAAAGCCTGTCAGTCAATCAGGAGGTGGTCGACTCATCACATCCTGGGGTTGAAGAAGGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGTCAGGTTTGTCTACGAGGAACTTCAAAGAGCAGCCACACTTGGAGCCTACGACGAAGCACTGCGTAGGCTAGCGCGCAATGGCTTTCTCTGCGTTGGGCTCGCTTCTTCAAGCCGGAGCCCAACCTATACAAGACAAGGTGCTGCTCGCTCAGGAAGTGGATATGCTGTTAGCTCTACCGGAGATAGCCAGGTTATCGACGATTCTACAATAGGCGGCCGCTACCCGACGAGTCTTCTTCAGTCCTGCAAGTACTGCTGGACTCGTTTCGAGCGTGAGTCTCTACTGCTCCGAAAACTAAGGATGAAGAGAGTCGCACTGGGAGCTAGTGCTTGCTATCTAAGAATTGCTCCATGGATCTCATCGGAAATGTTTGATGCTTCTTGGGGCTTCTTTGGCGACCGTGAAGTCACCGGCTTCGTGACCCGAACACGATTGGTGCTGACTGACGGGACAGATAGGATTTCATCTAGGGATAGACAGAAGCAACTGACATAG

Coding sequence (CDS)

ATGAAAGGAAGTCCATTACTGAGAAAAGTGGTGATCTCGTCTTGCTTGCTGGGAGAGAGGAAGCTTCCGGACCACCTTTTCCAGCCAGATAGCGAGCGAAACGGCCTCGCTAACGCGCCCCTTATTGAAAACTCAAGGAAAGCCTGTCAGTCAATCAGGAGGTGGTCGACTCATCACATCCTGGGGTTGAAGAAGGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGTCAGGTTTGTCTACGAGGAACTTCAAAGAGCAGCCACACTTGGAGCCTACGACGAAGCACTGCGTAGGCTAGCGCGCAATGGCTTTCTCTGCGTTGGGCTCGCTTCTTCAAGCCGGAGCCCAACCTATACAAGACAAGGTGCTGCTCGCTCAGGAAGTGGATATGCTGTTAGCTCTACCGGAGATAGCCAGGTTATCGACGATTCTACAATAGGCGGCCGCTACCCGACGAGTCTTCTTCAGTCCTGCAAGTACTGCTGGACTCGTTTCGAGCGTGAGTCTCTACTGCTCCGAAAACTAAGGATGAAGAGAGTCGCACTGGGAGCTAGTGCTTGCTATCTAAGAATTGCTCCATGGATCTCATCGGAAATGTTTGATGCTTCTTGGGGCTTCTTTGGCGACCGTGAAGTCACCGGCTTCGTGACCCGAACACGATTGGTGCTGACTGACGGGACAGATAGGATTTCATCTAGGGATAGACAGAAGCAACTGACATAG

Protein sequence

MKGSPLLRKVVISSCLLGERKLPDHLFQPDSERNGLANAPLIENSRKACQSIRRWSTHHILGLKKVPRVRLFADSSVRFVYEELQRAATLGAYDEALRRLARNGFLCVGLASSSRSPTYTRQGAARSGSGYAVSSTGDSQVIDDSTIGGRYPTSLLQSCKYCWTRFERESLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGDREVTGFVTRTRLVLTDGTDRISSRDRQKQLT
Homology
[Close]
BLAST of MC05g_new0320 vs. ExPASy Swiss-Prot
Match: P29388 (NADH-ubiquinone oxidoreductase chain 5 OS=Arabidopsis thaliana OX=3702 GN=ND5 PE=1 SV=3)

HSP 1 Score: 62.8 bits (151), Expect = 6.2e-09
Identity = 30/41 (73.17%), Postives = 32/41 (78.05%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VALGASACYLRIAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVALGASACYLRIAPWISSEMFDASWGFLFD 79

[Close]
BLAST of MC05g_new0320 vs. ExPASy Swiss-Prot
Match: Q37680 (NADH-ubiquinone oxidoreductase chain 5 OS=Triticum aestivum OX=4565 GN=ND5 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.2e-09
Identity = 30/41 (73.17%), Postives = 32/41 (78.05%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VALGASACYLRIAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVALGASACYLRIAPWISSEMFDASWGFLFD 79

[Close]
BLAST of MC05g_new0320 vs. ExPASy Swiss-Prot
Match: P10330 (NADH-ubiquinone oxidoreductase chain 5 OS=Oenothera berteroana OX=3950 GN=ND5 PE=2 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-08
Identity = 29/41 (70.73%), Postives = 32/41 (78.05%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VALGASACYL+IAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVALGASACYLKIAPWISSEMFDASWGFVFD 79

[Close]
BLAST of MC05g_new0320 vs. ExPASy Swiss-Prot
Match: P26849 (NADH-ubiquinone oxidoreductase chain 5 OS=Marchantia polymorpha OX=3197 GN=ND5 PE=3 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 22/30 (73.33%), Postives = 26/30 (86.67%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGD 211
           VAL ASACY++IAPWI SE+FDA+WGF  D
Sbjct: 50  VALCASACYIKIAPWIFSELFDAAWGFLFD 79

[Close]
BLAST of MC05g_new0320 vs. NCBI nr
Match: KAF6982729.1 (hypothetical protein CFC21_001077 [Triticum aestivum] >KAF6982732.1 hypothetical protein CFC21_001080 [Triticum aestivum])

HSP 1 Score: 80.1 bits (196), Expect = 1.66e-15
Identity = 38/46 (82.61%), Postives = 39/46 (84.78%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           S +L  L    VALGASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 39  SSILSFLAFNEVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. NCBI nr
Match: ANI86455.1 (NADH dehydrogenase subunit 5 [Hordeum vulgare subsp. vulgare] >EHK62709.1 hypothetical protein M3S_K08, partial [Sorghum bicolor] >KAF7008736.1 hypothetical protein CFC21_023434 [Triticum aestivum] >KAF7104930.1 hypothetical protein CFC21_105793 [Triticum aestivum])

HSP 1 Score: 79.0 bits (193), Expect = 4.59e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VALGASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 50  VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. NCBI nr
Match: TYK30811.1 (NADH dehydrogenase subunit 5 [Cucumis melo var. makuwa])

HSP 1 Score: 77.8 bits (190), Expect = 1.26e-14
Identity = 34/35 (97.14%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VALGASACYLRI+PWISSEMFDASWGFFGDREVTG
Sbjct: 50  VALGASACYLRISPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. NCBI nr
Match: BAT16545.1 (Os12g0251933 [Oryza sativa Japonica Group])

HSP 1 Score: 77.8 bits (190), Expect = 1.44e-14
Identity = 33/37 (89.19%), Postives = 36/37 (97.30%), Query Frame = 0 

Query: 179 KRVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           +++ALGASACYLRIAPWIS EMFDASWGFFGDREVTG
Sbjct: 53  RKIALGASACYLRIAPWISLEMFDASWGFFGDREVTG 89

[Close]
BLAST of MC05g_new0320 vs. NCBI nr
Match: QGW48345.1 (hypothetical protein [Raphanus sativus] >QGW48663.1 hypothetical protein [Raphanus sativus] >VDD25087.1 unnamed protein product [Brassica rapa] >VDD27136.1 unnamed protein product [Brassica rapa] >VDD65191.1 unnamed protein product [Brassica oleracea])

HSP 1 Score: 76.3 bits (186), Expect = 4.87e-14
Identity = 34/35 (97.14%), Postives = 34/35 (97.14%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VA GASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 50  VAPGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match: A0A3B5XW74 (Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 8.06e-16
Identity = 38/46 (82.61%), Postives = 39/46 (84.78%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           S +L  L    VALGASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 39  SSILSFLAFNEVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match: H0I4F2 (Uncharacterized protein (Fragment) OS=Sorghum bicolor OX=4558 GN=M3S_K08 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.22e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VALGASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 50  VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match: A0A191TDL7 (NADH dehydrogenase subunit 5 OS=Hordeum vulgare subsp. vulgare OX=112509 GN=nad5 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.22e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VALGASACYLRIAPWISSEMFDASWGFFGDREVTG
Sbjct: 50  VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match: A0A5D3E5D8 (NADH dehydrogenase subunit 5 (Mitochondrion) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76685G00010 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.12e-15
Identity = 34/35 (97.14%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 181 VALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           VALGASACYLRI+PWISSEMFDASWGFFGDREVTG
Sbjct: 50  VALGASACYLRISPWISSEMFDASWGFFGDREVTG 84

[Close]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match: A0A0P0Y8N0 (Os12g0251933 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0251933 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.99e-15
Identity = 33/37 (89.19%), Postives = 36/37 (97.30%), Query Frame = 0 

Query: 179 KRVALGASACYLRIAPWISSEMFDASWGFFGDREVTG 215
           +++ALGASACYLRIAPWIS EMFDASWGFFGDREVTG
Sbjct: 53  RKIALGASACYLRIAPWISLEMFDASWGFFGDREVTG 89

[Close]
BLAST of MC05g_new0320 vs. TAIR 10
Match: ATMG00060.1 (NADH dehydrogenase subunit 5C )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 29/41 (70.73%), Postives = 31/41 (75.61%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VA GASACYLRIAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVAPGASACYLRIAPWISSEMFDASWGFLFD 79

[Close]
BLAST of MC05g_new0320 vs. TAIR 10
Match: ATMG00513.1 (NADH dehydrogenase 5A )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 29/41 (70.73%), Postives = 31/41 (75.61%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VA GASACYLRIAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVAPGASACYLRIAPWISSEMFDASWGFLFD 79

[Close]
BLAST of MC05g_new0320 vs. TAIR 10
Match: ATMG00665.1 (NADH dehydrogenase 5B )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 29/41 (70.73%), Postives = 31/41 (75.61%), Query Frame = 0 

Query: 170 SLLLRKLRMKRVALGASACYLRIAPWISSEMFDASWGFFGD 211
           S +L  +    VA GASACYLRIAPWISSEMFDASWGF  D
Sbjct: 39  SSILSLIAFYEVAPGASACYLRIAPWISSEMFDASWGFLFD 79

The following BLAST results are available for this feature:
BLAST of MC05g_new0320 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P293886.2e-0973.17NADH-ubiquinone oxidoreductase chain 5 OS=Arabidopsis thaliana OX=3702 GN=ND5 PE... [more]
Q376806.2e-0973.17NADH-ubiquinone oxidoreductase chain 5 OS=Triticum aestivum OX=4565 GN=ND5 PE=3 ... [more]
P103301.8e-0870.73NADH-ubiquinone oxidoreductase chain 5 OS=Oenothera berteroana OX=3950 GN=ND5 PE... [more]
P268491.1e-0573.33NADH-ubiquinone oxidoreductase chain 5 OS=Marchantia polymorpha OX=3197 GN=ND5 P... [more]
BLAST of MC05g_new0320 vs. NCBI nr
Match NameE-valueIdentityDescription
KAF6982729.11.66e-1582.61hypothetical protein CFC21_001077 [Triticum aestivum] >KAF6982732.1 hypothetical... [more]
ANI86455.14.59e-15100.00NADH dehydrogenase subunit 5 [Hordeum vulgare subsp. vulgare] >EHK62709.1 hypoth... [more]
TYK30811.11.26e-1497.14NADH dehydrogenase subunit 5 [Cucumis melo var. makuwa][more]
BAT16545.11.44e-1489.19Os12g0251933 [Oryza sativa Japonica Group][more]
QGW48345.14.87e-1497.14hypothetical protein [Raphanus sativus] >QGW48663.1 hypothetical protein [Raphan... [more]
BLAST of MC05g_new0320 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A3B5XW748.06e-1682.61Uncharacterized protein OS=Triticum aestivum OX=4565 PE=4 SV=1[more]
H0I4F22.22e-15100.00Uncharacterized protein (Fragment) OS=Sorghum bicolor OX=4558 GN=M3S_K08 PE=4 SV... [more]
A0A191TDL72.22e-15100.00NADH dehydrogenase subunit 5 OS=Hordeum vulgare subsp. vulgare OX=112509 GN=nad5... [more]
A0A5D3E5D86.12e-1597.14NADH dehydrogenase subunit 5 (Mitochondrion) OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0P0Y8N06.99e-1589.19Os12g0251933 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0251933 PE... [more]
BLAST of MC05g_new0320 vs. TAIR 10
Match NameE-valueIdentityDescription
ATMG00060.12.9e-0970.73NADH dehydrogenase subunit 5C [more]
ATMG00513.12.9e-0970.73NADH dehydrogenase 5A [more]
ATMG00665.12.9e-0970.73NADH dehydrogenase 5B [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g_new0320.1MC05g_new0320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016491 oxidoreductase activity