MC05g_new0295 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0295
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	LRR receptor-like serine/threonine-protein kinase GSO1
Location	MC05: 14958280 .. 14958906 (+)
RNA-Seq Expression	MC05g_new0295
Synteny	MC05g_new0295

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCGAGACGCGCTTCTGAGATTCAAGAAAGAGCTCCAAGATTCGTCAAATCGTCTTTCTTCTTGGATTCCTCAAACAGATTGTTGCGAATGGGTTGGAATTCGTTGCGATAGTTTCACTGGCCATGTCAAAGAACTCAATCTTGCGAATACTCGATATGTTATTTGGGAAACAGAAGCAGAGAGGATTTCTTTCGAAAACTACAAGTTGAAGGGTAATTTGAGCGACTCGATCTTGAATTTAAAGCATGCAAGTCACATCGATCAAAGTTACAACGATTTTGGAGGGTTGCGAATTCCTGATTTTTTGGGGTCACTTGTGAGTTTGAATTATCTTAACCTCACAGGGTCAGGATTTCAAGGACTCATACCTCATCAGCTTGGAAATCTATCAAACTTGCAACAACTTGGTTTGCGAGGTAATTCTCCATTTTCTGGACCTAGATCGAATAGTGGAAATCTTCAATGGCTTCAAGGTCTTCGTTCATTGTTGAGCTTGGATTTGAGCTATGCAAACCTTAGAAAAGTGTCTAATTGGTTGCTAGATATTAATAAGCTTCCTTCTCTCGTGGAACTGCGTTTGTCTTACTGTGAATTGGGTCATATTACTCAAATTAGATATTAA

mRNA sequence

ATGGAGCGAGACGCGCTTCTGAGATTCAAGAAAGAGCTCCAAGATTCGTCAAATCGTCTTTCTTCTTGGATTCCTCAAACAGATTGTTGCGAATGGGTTGGAATTCGTTGCGATAGTTTCACTGGCCATGTCAAAGAACTCAATCTTGCGAATACTCGATATGTTATTTGGGAAACAGAAGCAGAGAGGATTTCTTTCGAAAACTACAAGTTGAAGGGTAATTTGAGCGACTCGATCTTGAATTTAAAGCATGCAAGTCACATCGATCAAAGTTACAACGATTTTGGAGGGTTGCGAATTCCTGATTTTTTGGGGTCACTTGTGAGTTTGAATTATCTTAACCTCACAGGGTCAGGATTTCAAGGACTCATACCTCATCAGCTTGGAAATCTATCAAACTTGCAACAACTTGGTTTGCGAGGTAATTCTCCATTTTCTGGACCTAGATCGAATAGTGGAAATCTTCAATGGCTTCAAGGTCTTCGTTCATTGTTGAGCTTGGATTTGAGCTATGCAAACCTTAGAAAAGTGTCTAATTGGTTGCTAGATATTAATAAGCTTCCTTCTCTCGTGGAACTGCGTTTGTCTTACTGTGAATTGGGTCATATTACTCAAATTAGATATTAA

Coding sequence (CDS)

ATGGAGCGAGACGCGCTTCTGAGATTCAAGAAAGAGCTCCAAGATTCGTCAAATCGTCTTTCTTCTTGGATTCCTCAAACAGATTGTTGCGAATGGGTTGGAATTCGTTGCGATAGTTTCACTGGCCATGTCAAAGAACTCAATCTTGCGAATACTCGATATGTTATTTGGGAAACAGAAGCAGAGAGGATTTCTTTCGAAAACTACAAGTTGAAGGGTAATTTGAGCGACTCGATCTTGAATTTAAAGCATGCAAGTCACATCGATCAAAGTTACAACGATTTTGGAGGGTTGCGAATTCCTGATTTTTTGGGGTCACTTGTGAGTTTGAATTATCTTAACCTCACAGGGTCAGGATTTCAAGGACTCATACCTCATCAGCTTGGAAATCTATCAAACTTGCAACAACTTGGTTTGCGAGGTAATTCTCCATTTTCTGGACCTAGATCGAATAGTGGAAATCTTCAATGGCTTCAAGGTCTTCGTTCATTGTTGAGCTTGGATTTGAGCTATGCAAACCTTAGAAAAGTGTCTAATTGGTTGCTAGATATTAATAAGCTTCCTTCTCTCGTGGAACTGCGTTTGTCTTACTGTGAATTGGGTCATATTACTCAAATTAGATATTAA

Protein sequence

MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEAERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWLLDINKLPSLVELRLSYCELGHITQIRY

Homology

[Close]

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot
Match: Q6JN47 (Receptor-like protein EIX1 OS=Solanum lycopersicum OX=4081 GN=EIX1 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 5.9e-32
Identity = 87/205 (42.44%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTD---CCEWVGIRCDSFTGHVKELNLANTRYVIWE 61
           ERDALL FK+ L DS + LS+W  + D   CC+W GI CD  TGHV  ++L N     + 
Sbjct: 38  ERDALLEFKRGLTDSFDHLSTWGDEEDKQECCKWKGIECDRRTGHVTVIDLHNK----FT 97

Query: 62  TEAERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGS 121
             A   +    +L G LS S+L L++ +++D S N+F    IP F+GSL  L YLNL+ S
Sbjct: 98  CSAGASACFAPRLTGKLSPSLLELEYLNYLDLSVNEFERSEIPRFIGSLKRLEYLNLSAS 157

Query: 122 GFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVS 181
            F G+IP Q  NL++L+ L L  N+          +L+WL  L SL  L LS +N  +V+
Sbjct: 158 FFSGVIPIQFQNLTSLRTLDLGENNLI------VKDLRWLSHLSSLEFLSLSSSNF-QVN 217

Query: 182 NWLLDINKLPSLVELRLSYCELGHI 204
           NW  +I K+PSL EL LS C L  +
Sbjct: 218 NWFQEITKVPSLKELDLSGCGLSKL 231

[Close]

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot
Match: Q6JN46 (Receptor-like protein EIX2 OS=Solanum lycopersicum OX=4081 GN=EIX2 PE=1 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 83/199 (41.71%), Postives = 106/199 (53.27%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ERDALL FK+ L D   RLS+W  + +CC W GI CD  TGHV  L+L +      E   
Sbjct: 39  ERDALLEFKRGLNDDFGRLSTWGDEEECCNWKGIECDKRTGHVIVLDLHS------EVTC 98

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
              +     L G +S S+L L++ + +D S N F    IP F+GSL  L YLNL+ S F 
Sbjct: 99  PGHACFAPILTGKVSPSLLELEYLNFLDLSVNGFENSEIPRFIGSLKRLEYLNLSSSDFS 158

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWL 181
           G IP Q  NL++L+ L L  N+          +L WL  L SL  L L   N  +  NW 
Sbjct: 159 GEIPAQFQNLTSLRILDLGNNNLI------VKDLVWLSHLSSLEFLRLG-GNDFQARNWF 218

Query: 182 LDINKLPSLVELRLSYCEL 201
            +I K+PSL EL LS C L
Sbjct: 219 REITKVPSLKELDLSVCGL 224

[Close]

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot
Match: Q9SRL2 (Receptor-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=RLP34 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 71/215 (33.02%), Postives = 105/215 (48.84%), Query Frame = 0


Query: 2   ERDALLRFKKELQ-------------DSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELN 61
           ++DALL+FK E +             +S  +  SW   +DCC W G+ C++ +G V ELN
Sbjct: 42  QKDALLKFKNEFEIGKPSPTCKMVGIESHRKTESWGNNSDCCNWEGVTCNAKSGEVIELN 101

Query: 62  L------------ANTRYVIWETEAERISFENYKLKGNLSDSILNLKHASHIDQSYNDFG 121
           L            ++ R + + T  +R    +   +G ++ SI NL H + +D SYN F 
Sbjct: 102 LSCSSLHGRFHSNSSIRNLHFLTTLDR---SHNDFEGQITSSIENLSHLTSLDLSYNRFS 161

Query: 122 GLRIPDFLGSLVSLNYLNLTGSGFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQ 181
           G +I + +G+L  L  L+L+ + F G IP  +GNLS+L  LGL GN  F    S+ GNL 
Sbjct: 162 G-QILNSIGNLSRLTSLDLSFNQFSGQIPSSIGNLSHLTFLGLSGNRFFGQIPSSIGNLS 221

Query: 182 -----WLQGLRSLLSLDLSYANLRKVSNWLLDINK 187
                 L G R       S   L  ++N  L  NK
Sbjct: 222 HLTFLGLSGNRFFGQFPSSIGGLSNLTNLHLSYNK 252

[Close]

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot
Match: F4J8G2 (Receptor-like protein 33 OS=Arabidopsis thaliana OX=3702 GN=RLP33 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-18
Identity = 77/216 (35.65%), Postives = 104/216 (48.15%), Query Frame = 0


Query: 2   ERDALLRFKKELQ--------DSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTR 61
           +RDALL FK E +         S  +  SW   +DCC W GI CD+ TG V E++L  + 
Sbjct: 34  QRDALLEFKNEFKIKKPCFGCPSPLKTKSWENGSDCCHWDGITCDAKTGEVIEIDLMCSC 93

Query: 62  YVIWETEAERIS-FENY-----------KLKGNLSDSILNLKHASHIDQSYNDFGGLRIP 121
              W      +S  +N+            L G +S SI NL H + +D S N+F G  IP
Sbjct: 94  LHGWFHSNSNLSMLQNFHFLTTLDLSYNHLSGQISSSIGNLSHLTTLDLSGNNFSGW-IP 153

Query: 122 DFLGSLVSLNYLNLTGSGFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGL 181
             LG+L  L  L+L  + F G IP  LGNLS L  L L  N+      S+ G+L  L   
Sbjct: 154 SSLGNLFHLTSLHLYDNNFGGEIPSSLGNLSYLTFLDLSTNNFVGEIPSSFGSLNQL--- 213

Query: 182 RSLLSLDLSYANLRKVSNWLLDINKLPSLVELRLSY 198
            S+L LD    N +   N  L++  L  L E+ LS+
Sbjct: 214 -SILRLD----NNKLSGNLPLEVINLTKLSEISLSH 240

[Close]

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot
Match: Q9SRL7 (Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 78/248 (31.45%), Postives = 109/248 (43.95%), Query Frame = 0


Query: 2   ERDALLRFKKELQ---DSSN--------RLS------SWIPQTDCCEWVGIRCDSFTGHV 61
           +RDALL  K E +    SSN        R+S      SW   +DCC W GI CD+ +G V
Sbjct: 42  QRDALLELKNEFEIGKPSSNDYCYRNNSRVSPHPTTESWRNNSDCCNWEGITCDTKSGEV 101

Query: 62  KELNLA-NTRYVIWETEAERISFENYK--------LKGNLSDSILNLKHASHIDQSYNDF 121
            EL+L+ +  Y  + + +     +N +        L G +  SI NL H + +  SYN F
Sbjct: 102 IELDLSCSWLYGSFHSNSSLFRLQNLRVLDLTQNDLDGEIPSSIGNLSHLTSLHLSYNQF 161

Query: 122 GGL-----------------------RIPDFLGSLVSLNYLNLTGSGFQGLIPHQLGNLS 181
            GL                       +IP  +G+L  L  L L+ + F G IP  +GNLS
Sbjct: 162 LGLIPSSIENLSRLTSLHLSSNQFSGQIPSSIGNLSHLTSLELSSNQFSGQIPSSIGNLS 221

Query: 182 NLQQLGLRGNSPFSGPRSNSGNLQWLQGL-----RSLLSLDLSYANLRKVSNWLLDINKL 196
           NL  L L  N  F    S+ GNL  L  L       +  +  S+ NL ++    +D NKL
Sbjct: 222 NLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEIPSSFGNLNQLIVLQVDSNKL 281

[Close]

BLAST of MC05g_new0295 vs. NCBI nr
Match: XP_022154250.1 (LRR receptor-like serine/threonine-protein kinase GSO1 [Momordica charantia])

HSP 1 Score: 416 bits (1070), Expect = 2.08e-138
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE 60
           MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE
Sbjct: 1   MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE 60

Query: 61  AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF 120
           AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF
Sbjct: 61  AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF 120

Query: 121 QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW 180
           QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW
Sbjct: 121 QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW 180

Query: 181 LLDINKLPSLVELRLSYCELGHITQI 206
           LLDINKLPSLVELRLSYCELGHIT +
Sbjct: 181 LLDINKLPSLVELRLSYCELGHITPL 206

[Close]

BLAST of MC05g_new0295 vs. NCBI nr
Match: XP_022159203.1 (LRR receptor-like serine/threonine-protein kinase GSO2 [Momordica charantia])

HSP 1 Score: 281 bits (718), Expect = 1.24e-84
Identity = 145/207 (70.05%), Postives = 164/207 (79.23%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ERDALLR K+ELQDSSNRLSSW+  TDCC+W GIRCD+ TGHV+EL+LANT +       
Sbjct: 30  ERDALLRLKQELQDSSNRLSSWMVGTDCCQWAGIRCDNSTGHVEELDLANTEF------E 89

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
           +  SFE++KLKGNLSDSILNLKH +HID SYNDF G +IP FLGSLVSL YLNLT SGFQ
Sbjct: 90  DMNSFESHKLKGNLSDSILNLKHITHIDLSYNDFEGRQIPSFLGSLVSLKYLNLTSSGFQ 149

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWL 181
           GLIPHQLGNLSNLQQL LRG   +S       +LQWLQGL SLLSLDLS+ NL K +NWL
Sbjct: 150 GLIPHQLGNLSNLQQLSLRGKLLYSE------DLQWLQGLPSLLSLDLSHTNLSKATNWL 209

Query: 182 LDINKLPSLVELRLSYCELGHITQIRY 208
           L+INKLP LVEL LS CEL HIT + +
Sbjct: 210 LEINKLPCLVELHLSNCELNHITHLSH 224

[Close]

BLAST of MC05g_new0295 vs. NCBI nr
Match: XP_038882430.1 (receptor-like protein EIX1 [Benincasa hispida])

HSP 1 Score: 278 bits (710), Expect = 1.90e-83
Identity = 145/208 (69.71%), Postives = 160/208 (76.92%), Query Frame = 0


Query: 1   MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE 60
           +ER+ALL+FK+ L D SNRLSSW+  TDCC+W  IRCDS TGHVK+LNL N R+V W T 
Sbjct: 19  IEREALLKFKEVLVDPSNRLSSWLVGTDCCKWAAIRCDSLTGHVKQLNLGN-RFVNWNTL 78

Query: 61  AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF 120
            E  SF N KLKG+LSDSILNL H S ID SYNDFGG+ IP F GSL SLNYLNLT SGF
Sbjct: 79  KEMNSFLNDKLKGSLSDSILNLSHISRIDLSYNDFGGIPIPSFFGSLTSLNYLNLTSSGF 138

Query: 121 QGLIPHQLGNLSNLQQLGLRGNSPFSGPRS--NSGNLQWLQGLRSLLSLDLSYANLRKVS 180
           QGL+P+QLGNLSNLQ L LRGN  F        S NLQWLQGLRSLLSLDLS  NL KVS
Sbjct: 139 QGLVPYQLGNLSNLQYLSLRGNFRFLDDAKLLYSENLQWLQGLRSLLSLDLSQVNLSKVS 198

Query: 181 NWLLDINKLPSLVELRLSYCELGHITQI 206
           NWLL+INKLP LVEL +S CEL HIT +
Sbjct: 199 NWLLEINKLPYLVELHMSGCELNHITPL 225

[Close]

BLAST of MC05g_new0295 vs. NCBI nr
Match: XP_023552839.1 (receptor like protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229 bits (583), Expect = 1.77e-71
Identity = 124/199 (62.31%), Postives = 141/199 (70.85%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ERDALLRFKKEL +  NRL SW  + DCC+WVGIRCD+ TG+VKEL LAN  +       
Sbjct: 33  ERDALLRFKKELVNPLNRLWSWDVEQDCCKWVGIRCDNSTGYVKELILANNEFS------ 92

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
                E Y LKGN+SDSILNLKH +H D S NDF G+ IP FLGS+VSL YL+L+ +GF 
Sbjct: 93  -----EAYPLKGNVSDSILNLKHLTHFDLSNNDFEGVGIPSFLGSMVSLRYLDLSRAGFS 152

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWL 181
           GLIPH+LGNLSNL  L LR       P+  + NL WL GL SLL LDLSY NL K S+WL
Sbjct: 153 GLIPHELGNLSNLNHLDLRSTLI---PQLYAENLGWLHGLPSLLKLDLSYTNLSKASSWL 212

Query: 182 LDINKLPSLVELRLSYCEL 200
           LDINKLPSL EL LS CEL
Sbjct: 213 LDINKLPSLQELHLSGCEL 217

[Close]

BLAST of MC05g_new0295 vs. NCBI nr
Match: XP_022140821.1 (LRR receptor-like serine/threonine-protein kinase GSO2 [Momordica charantia])

HSP 1 Score: 239 bits (609), Expect = 1.66e-69
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 60  EAERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSG 119
           EAE I+ ENY LKGNLSDSI+NL+H S ID SYNDFGG++IP FLGSLVSLNYLNLTGSG
Sbjct: 14  EAEWITSENYTLKGNLSDSIVNLRHVSRIDLSYNDFGGIQIPSFLGSLVSLNYLNLTGSG 73

Query: 120 FQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSN 179
           FQGLIPHQLGNLSNLQQLGLRG SPFSGP+  S NL WLQGL SLLSLDLSYANL K S+
Sbjct: 74  FQGLIPHQLGNLSNLQQLGLRGKSPFSGPKLYSENLHWLQGLGSLLSLDLSYANLSKASD 133

Query: 180 WLLDINKLPSLVELRLSYCELGHITQIRY 208
           WLL+INKLPSLVELRLS CEL HIT + +
Sbjct: 134 WLLEINKLPSLVELRLSNCELSHITPLNH 162

[Close]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DJ40 (LRR receptor-like serine/threonine-protein kinase GSO1 OS=Momordica charantia OX=3673 GN=LOC111021548 PE=4 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 1.01e-138
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE 60
           MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE
Sbjct: 1   MERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETE 60

Query: 61  AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF 120
           AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF
Sbjct: 61  AERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGF 120

Query: 121 QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW 180
           QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW
Sbjct: 121 QGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNW 180

Query: 181 LLDINKLPSLVELRLSYCELGHITQI 206
           LLDINKLPSLVELRLSYCELGHIT +
Sbjct: 181 LLDINKLPSLVELRLSYCELGHITPL 206

[Close]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1DY64 (LRR receptor-like serine/threonine-protein kinase GSO2 OS=Momordica charantia OX=3673 GN=LOC111025622 PE=4 SV=1)

HSP 1 Score: 281 bits (718), Expect = 5.99e-85
Identity = 145/207 (70.05%), Postives = 164/207 (79.23%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ERDALLR K+ELQDSSNRLSSW+  TDCC+W GIRCD+ TGHV+EL+LANT +       
Sbjct: 30  ERDALLRLKQELQDSSNRLSSWMVGTDCCQWAGIRCDNSTGHVEELDLANTEF------E 89

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
           +  SFE++KLKGNLSDSILNLKH +HID SYNDF G +IP FLGSLVSL YLNLT SGFQ
Sbjct: 90  DMNSFESHKLKGNLSDSILNLKHITHIDLSYNDFEGRQIPSFLGSLVSLKYLNLTSSGFQ 149

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWL 181
           GLIPHQLGNLSNLQQL LRG   +S       +LQWLQGL SLLSLDLS+ NL K +NWL
Sbjct: 150 GLIPHQLGNLSNLQQLSLRGKLLYSE------DLQWLQGLPSLLSLDLSHTNLSKATNWL 209

Query: 182 LDINKLPSLVELRLSYCELGHITQIRY 208
           L+INKLP LVEL LS CEL HIT + +
Sbjct: 210 LEINKLPCLVELHLSNCELNHITHLSH 224

[Close]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1CH67 (LRR receptor-like serine/threonine-protein kinase GSO2 OS=Momordica charantia OX=3673 GN=LOC111011398 PE=4 SV=1)

HSP 1 Score: 239 bits (609), Expect = 8.02e-70
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 60  EAERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSG 119
           EAE I+ ENY LKGNLSDSI+NL+H S ID SYNDFGG++IP FLGSLVSLNYLNLTGSG
Sbjct: 14  EAEWITSENYTLKGNLSDSIVNLRHVSRIDLSYNDFGGIQIPSFLGSLVSLNYLNLTGSG 73

Query: 120 FQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSN 179
           FQGLIPHQLGNLSNLQQLGLRG SPFSGP+  S NL WLQGL SLLSLDLSYANL K S+
Sbjct: 74  FQGLIPHQLGNLSNLQQLGLRGKSPFSGPKLYSENLHWLQGLGSLLSLDLSYANLSKASD 133

Query: 180 WLLDINKLPSLVELRLSYCELGHITQIRY 208
           WLL+INKLPSLVELRLS CEL HIT + +
Sbjct: 134 WLLEINKLPSLVELRLSNCELSHITPLNH 162

[Close]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL
Match: A0A2N9J8V7 (LRRNT_2 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61658 PE=4 SV=1)

HSP 1 Score: 206 bits (524), Expect = 4.53e-63
Identity = 109/209 (52.15%), Postives = 143/209 (68.42%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYV----IW 61
           ER ALLRFK+ LQD SNRL+SW+   DCC+WVG+ C + TGHV EL+L +   V    + 
Sbjct: 38  ERQALLRFKQHLQDPSNRLASWVGDGDCCQWVGVVCHNVTGHVHELHLRSFPPVRDDFVT 97

Query: 62  ETEAERISFENYKLK---GNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLN 121
           + E  R  +  Y L    G ++ S+L+LKH +++D SYNDF  ++IP+FLGS+ SL YLN
Sbjct: 98  DEEQYRAQYGAYMLSMFGGKINHSLLDLKHLNYLDLSYNDFSDIQIPEFLGSMGSLRYLN 157

Query: 122 LTGSGFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANL 181
           L+ + FQG+IPHQLGNLSNL  L L GN  ++       NLQWL GL  L  LD+S+ +L
Sbjct: 158 LSNARFQGVIPHQLGNLSNLHYLNLAGNGLYAM------NLQWLSGLPLLQYLDMSHVDL 217

Query: 182 RKVSNWLLDINKLPSLVELRLSYCELGHI 203
            K S+WL  INKLPSL++L+LSYCEL  I
Sbjct: 218 SKASDWLQVINKLPSLLDLQLSYCELRFI 240

[Close]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL
Match: A0A6J1E6S3 (LRR receptor-like serine/threonine-protein kinase FLS2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430483 PE=4 SV=1)

HSP 1 Score: 218 bits (554), Expect = 5.30e-62
Identity = 118/199 (59.30%), Postives = 139/199 (69.85%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ER+ALLRFKK L D  NRLSSW  + DCC+WVGI CD+ TG+VKEL LAN  ++      
Sbjct: 33  EREALLRFKKGLVDPLNRLSSWDVKQDCCKWVGISCDNSTGYVKELILANNEFL------ 92

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
                E Y+LKGN+SDSILNLKH +H D S NDF G+RIP FLGS+VSL YLNL+ + F 
Sbjct: 93  -----EAYQLKGNVSDSILNLKHLTHFDLSNNDFEGVRIPSFLGSMVSLRYLNLSRARFS 152

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGLRSLLSLDLSYANLRKVSNWL 181
           GLIPH+LGNLSNL  LGL G  P       + NL WL GL SLL L+L + +L   S+WL
Sbjct: 153 GLIPHELGNLSNLNHLGLGGTYP----PLYTENLGWLHGLPSLLKLNLMHVDLSNASSWL 212

Query: 182 LDINKLPSLVELRLSYCEL 200
           LDINKLPSL EL L  C+L
Sbjct: 213 LDINKLPSLQELYLWRCKL 216

[Close]

BLAST of MC05g_new0295 vs. TAIR 10
Match: AT2G34930.1 (disease resistance family protein / LRR family protein )

HSP 1 Score: 141.4 bits (355), Expect = 8.5e-34
Identity = 88/206 (42.72%), Postives = 117/206 (56.80%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           ER ALL F+  L D S+RL SW    DCC W G+ CD+ T HV +++L N    +   E 
Sbjct: 37  ERQALLTFRAALTDLSSRLFSW-SGPDCCNWPGVLCDARTSHVVKIDLRNPSQDVRSDEY 96

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
           +R S     L+G +  S+  LK  S++D S NDF  L IP+F+G +VSL YLNL+ S F 
Sbjct: 97  KRGS-----LRGKIHPSLTQLKFLSYLDLSSNDFNELEIPEFIGQIVSLRYLNLSSSSFS 156

Query: 122 GLIPHQLGNLSNLQQLGLRGNS-PFSGPRS-NSGNLQWLQGLRSLLS-LDLSYANLRKV- 181
           G IP  LGNLS L+ L L   S   SG  S  + NL+WL  L S L  L++ Y NL    
Sbjct: 157 GEIPTSLGNLSKLESLDLYAESFGDSGTLSLRASNLRWLSSLSSSLKYLNMGYVNLSGAG 216

Query: 182 SNWLLDINKLPSLVELRLSYCELGHI 204
             WL D +++ +L EL L   EL ++
Sbjct: 217 ETWLQDFSRISALKELHLFNSELKNL 236

[Close]

BLAST of MC05g_new0295 vs. TAIR 10
Match: AT3G05660.1 (receptor like protein 33 )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 77/216 (35.65%), Postives = 104/216 (48.15%), Query Frame = 0


Query: 2   ERDALLRFKKELQ--------DSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTR 61
           +RDALL FK E +         S  +  SW   +DCC W GI CD+ TG V E++L  + 
Sbjct: 34  QRDALLEFKNEFKIKKPCFGCPSPLKTKSWENGSDCCHWDGITCDAKTGEVIEIDLMCSC 93

Query: 62  YVIWETEAERIS-FENY-----------KLKGNLSDSILNLKHASHIDQSYNDFGGLRIP 121
              W      +S  +N+            L G +S SI NL H + +D S N+F G  IP
Sbjct: 94  LHGWFHSNSNLSMLQNFHFLTTLDLSYNHLSGQISSSIGNLSHLTTLDLSGNNFSGW-IP 153

Query: 122 DFLGSLVSLNYLNLTGSGFQGLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGL 181
             LG+L  L  L+L  + F G IP  LGNLS L  L L  N+      S+ G+L  L   
Sbjct: 154 SSLGNLFHLTSLHLYDNNFGGEIPSSLGNLSYLTFLDLSTNNFVGEIPSSFGSLNQL--- 213

Query: 182 RSLLSLDLSYANLRKVSNWLLDINKLPSLVELRLSY 198
            S+L LD    N +   N  L++  L  L E+ LS+
Sbjct: 214 -SILRLD----NNKLSGNLPLEVINLTKLSEISLSH 240

[Close]

BLAST of MC05g_new0295 vs. TAIR 10
Match: AT3G11010.1 (receptor like protein 34 )

HSP 1 Score: 91.7 bits (226), Expect = 7.7e-19
Identity = 64/189 (33.86%), Postives = 94/189 (49.74%), Query Frame = 0


Query: 15  DSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNL------------ANTRYVIWETEAE 74
           +S  +  SW   +DCC W G+ C++ +G V ELNL            ++ R + + T  +
Sbjct: 5   ESHRKTESWGNNSDCCNWEGVTCNAKSGEVIELNLSCSSLHGRFHSNSSIRNLHFLTTLD 64

Query: 75  RISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQG 134
           R    +   +G ++ SI NL H + +D SYN F G +I + +G+L  L  L+L+ + F G
Sbjct: 65  R---SHNDFEGQITSSIENLSHLTSLDLSYNRFSG-QILNSIGNLSRLTSLDLSFNQFSG 124

Query: 135 LIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQ-----WLQGLRSLLSLDLSYANLRKV 187
            IP  +GNLS+L  LGL GN  F    S+ GNL       L G R       S   L  +
Sbjct: 125 QIPSSIGNLSHLTFLGLSGNRFFGQIPSSIGNLSHLTFLGLSGNRFFGQFPSSIGGLSNL 184

[Close]

BLAST of MC05g_new0295 vs. TAIR 10
Match: AT3G11080.1 (receptor like protein 35 )

HSP 1 Score: 88.2 bits (217), Expect = 8.5e-18
Identity = 78/248 (31.45%), Postives = 109/248 (43.95%), Query Frame = 0


Query: 2   ERDALLRFKKELQ---DSSN--------RLS------SWIPQTDCCEWVGIRCDSFTGHV 61
           +RDALL  K E +    SSN        R+S      SW   +DCC W GI CD+ +G V
Sbjct: 42  QRDALLELKNEFEIGKPSSNDYCYRNNSRVSPHPTTESWRNNSDCCNWEGITCDTKSGEV 101

Query: 62  KELNLA-NTRYVIWETEAERISFENYK--------LKGNLSDSILNLKHASHIDQSYNDF 121
            EL+L+ +  Y  + + +     +N +        L G +  SI NL H + +  SYN F
Sbjct: 102 IELDLSCSWLYGSFHSNSSLFRLQNLRVLDLTQNDLDGEIPSSIGNLSHLTSLHLSYNQF 161

Query: 122 GGL-----------------------RIPDFLGSLVSLNYLNLTGSGFQGLIPHQLGNLS 181
            GL                       +IP  +G+L  L  L L+ + F G IP  +GNLS
Sbjct: 162 LGLIPSSIENLSRLTSLHLSSNQFSGQIPSSIGNLSHLTSLELSSNQFSGQIPSSIGNLS 221

Query: 182 NLQQLGLRGNSPFSGPRSNSGNLQWLQGL-----RSLLSLDLSYANLRKVSNWLLDINKL 196
           NL  L L  N  F    S+ GNL  L  L       +  +  S+ NL ++    +D NKL
Sbjct: 222 NLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEIPSSFGNLNQLIVLQVDSNKL 281

[Close]

BLAST of MC05g_new0295 vs. TAIR 10
Match: AT1G71390.1 (receptor like protein 11 )

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-17
Identity = 67/211 (31.75%), Postives = 98/211 (46.45%), Query Frame = 0


Query: 2   ERDALLRFKKELQDSSNRLSSWIPQTDCCEWVGIRCDSFTGHVKELNLANTRYVIWETEA 61
           +RD LL+F+ E     ++ S W   TDCC W G+ CD  +G V  L+L +T         
Sbjct: 36  QRDGLLKFRDEFPIFESKSSPWNKTTDCCSWDGVTCDDKSGQVISLDLRST--------- 95

Query: 62  ERISFENYKLKGNLSDSILNLKHASHIDQSYNDFGGLRIPDFLGSLVSLNYLNLTGSGFQ 121
                 N  LK N   S+  L++  H+D S  +  G  IP  LG+L  L  L L+ +   
Sbjct: 96  ----LLNSSLKTN--SSLFRLQYLRHLDLSGCNLHG-EIPSSLGNLSRLENLELSSNRLV 155

Query: 122 GLIPHQLGNLSNLQQLGLRGNSPFSGPRSNSGNLQWLQGL----RSLL-SLDLSYANLRK 181
           G IP+ +GNL  L+ L L  N       S+ GNL  L  L     SL+  +  S  NL +
Sbjct: 156 GEIPYSIGNLKQLRNLSLGDNDLIGEIPSSLGNLSLLLDLDLWNNSLVGEVPASIGNLNE 215

Query: 182 VSNWLLDINKLPSLVELRLSYCELGHITQIR 208
           +    LD N L     + +S+  L  +++ R
Sbjct: 216 LRVMSLDRNSLSG--SIPISFTNLTKLSEFR 228

The following BLAST results are available for this feature:

BLAST of MC05g_new0295 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6JN47	5.9e-32	42.44	Receptor-like protein EIX1 OS=Solanum lycopersicum OX=4081 GN=EIX1 PE=2 SV=2	[more]
Q6JN46	1.8e-28	41.71	Receptor-like protein EIX2 OS=Solanum lycopersicum OX=4081 GN=EIX2 PE=1 SV=2	[more]
Q9SRL2	1.2e-19	33.02	Receptor-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=RLP34 PE=2 SV=1	[more]
F4J8G2	1.7e-18	35.65	Receptor-like protein 33 OS=Arabidopsis thaliana OX=3702 GN=RLP33 PE=3 SV=1	[more]
Q9SRL7	1.2e-16	31.45	Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1	[more]

BLAST of MC05g_new0295 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154250.1	2.08e-138	99.03	LRR receptor-like serine/threonine-protein kinase GSO1 [Momordica charantia]	[more]
XP_022159203.1	1.24e-84	70.05	LRR receptor-like serine/threonine-protein kinase GSO2 [Momordica charantia]	[more]
XP_038882430.1	1.90e-83	69.71	receptor-like protein EIX1 [Benincasa hispida]	[more]
XP_023552839.1	1.77e-71	62.31	receptor like protein 30-like [Cucurbita pepo subsp. pepo]	[more]
XP_022140821.1	1.66e-69	80.54	LRR receptor-like serine/threonine-protein kinase GSO2 [Momordica charantia]	[more]

BLAST of MC05g_new0295 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ40	1.01e-138	99.03	LRR receptor-like serine/threonine-protein kinase GSO1 OS=Momordica charantia OX...	[more]
A0A6J1DY64	5.99e-85	70.05	LRR receptor-like serine/threonine-protein kinase GSO2 OS=Momordica charantia OX...	[more]
A0A6J1CH67	8.02e-70	80.54	LRR receptor-like serine/threonine-protein kinase GSO2 OS=Momordica charantia OX...	[more]
A0A2N9J8V7	4.53e-63	52.15	LRRNT_2 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61658 ...	[more]
A0A6J1E6S3	5.30e-62	59.30	LRR receptor-like serine/threonine-protein kinase FLS2 isoform X1 OS=Cucurbita m...	[more]

BLAST of MC05g_new0295 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34930.1	8.5e-34	42.72	disease resistance family protein / LRR family protein	[more]
AT3G05660.1	1.2e-19	35.65	receptor like protein 33	[more]
AT3G11010.1	7.7e-19	33.86	receptor like protein 34	[more]
AT3G11080.1	8.5e-18	31.45	receptor like protein 35	[more]
AT1G71390.1	1.4e-17	31.75	receptor like protein 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 1..206 e-value: 1.4E-42 score: 147.3
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 2..38 e-value: 1.4E-14 score: 54.1
None	No IPR available	PANTHER	PTHR48063:SF48	DNA-DAMAGE-REPAIR/TOLERATION PROTEIN DRT100-LIKE	coord: 2..201
None	No IPR available	PANTHER	PTHR48063	LRR RECEPTOR-LIKE KINASE	coord: 2..201
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 2..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0295.1	MC05g_new0295.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016301	kinase activity