MC05g_new0150 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0150
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Reverse transcriptase domain-containing protein
Location	MC05: 8312654 .. 8313864 (-)
RNA-Seq Expression	MC05g_new0150
Synteny	MC05g_new0150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGATAATATGTCGAGAGGAGGAGCTGCCCAGGATGTTATTGAGGCTGAGCTCGCGCTTGAGAGGATTTCATCTGAAGAAGAGGTATACTGAAAGTCGCGGTCGAGGGAGAATTGGCTTAAAGTAGGGGACTGTAACACACGGTGGTTTCATAGCAAAGCTTCGTACAGGAAGAAGAAGAATGCCATTAGGGGGTTGGAGGATTCTTTTGGTGGGTGGACGAGCGACCCTGCAAAAATGGTGAATATTGTCTCTGATTACTTTAATGTTTTGTTCTCTACTTCAGTGTGTGCCGAGACATATTTTGATGATATCTTGTCCGAGGTTAGGGCGGTGATGATAGACAGGCATAATCAGCTCCAATTGAGACAGTTTAGCGCTCAAGAGTCGTTGTTGCTTTCCGCCAAATTGGGCCCCATAAGGCTCCTGGCCCTGATGGCCAGTGGTGGGGAATGAAGTTGTGCAGTGTTGAATAATGAGGTATCGCCCGCTCCACTTAATGAGACTTTGATTGTTCTTTTACCGAAGGTGCCTACCCCAAGGAGGATGGCTAAATTCCTATCGATTTCGTTGTGTAATGTATGTTATAAAATTATCTCGAAGGCTATTGTCAATCGTTTGAAAGATGTGTTGGACTCTGTTATCTCAAACAATCAGAGTGCATTTGTTCCTGGCAGATGTGTAGTGGATAATGTGATCTTGGGGTACGAGTGTGTGCATTCTATTCGGAACAGACGCTCCAAATCAGTGGGTGAGCAACTTTGAAGCTTGATATGATTAAGGCTTATGATCGTGTGAAGTGGTGTTTTCTTGAACAAATGATGTTTCGTATGAGCTTTGCTCGGCCATGGGTAGTTTGATCATGAGGTGTGTGACATCTGTCTCCTTTTCTTTTCTTTTGAATGGCGAGCGATATGGTAATATTATTCCATCTCGGGGTTTCGGGCAAGGAGATCCTCTTTCCCCCTATCTCTTTCTGATATGTGCTGAGGGGTTGTCTTGCAGTTTGAAGGGTGCGATTAGGCGGAAGCTTATATCTGTATTTGTTTTGTCTAGGGGAGGCCTTCCATTTCCCACCTCTTTTTAGTCGATGATAGTCTCTTGTTTTTCAGAGCAAATAAAGTGGAGGTTGAATGAGTTAAAGGGATTTTGCGGGCTTATGAGAGAACTTCTGGCCAAACCATTAATTTGATAAATCAGTTATATCTTTTAG

mRNA sequence

ATGGCTAAATTCCTATCGATTTCGTTGTGTAATGTATGTTATAAAATTATCTCGAAGGCTATTGTCAATCGTTTGAAAGATGTGTTGGACTCTGTTATCTCAAACAATCAGAGTGCATTTGTTCCTGGCAGATGTGTAGTGGATAATGTGATCTTGGGGTACGAGTGTGTGCATTCTATTCGGAACAGACGCTCCAAATCAGTGGGTGAGCAACTTTGA

Coding sequence (CDS)

ATGGCTAAATTCCTATCGATTTCGTTGTGTAATGTATGTTATAAAATTATCTCGAAGGCTATTGTCAATCGTTTGAAAGATGTGTTGGACTCTGTTATCTCAAACAATCAGAGTGCATTTGTTCCTGGCAGATGTGTAGTGGATAATGTGATCTTGGGGTACGAGTGTGTGCATTCTATTCGGAACAGACGCTCCAAATCAGTGGGTGAGCAACTTTGA

Protein sequence

MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSIRNRRSKSVGEQL

Homology

[Close]

BLAST of MC05g_new0150 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 3.6e-05
Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 0


Query: 7   ISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSIR 62
           +SL +  YKI++KAI  RLK VL  VI  +QS  VPGR + DNV L  + +H  R
Sbjct: 528 VSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFAR 582

[Close]

BLAST of MC05g_new0150 vs. NCBI nr
Match: XP_022149385.1 (uncharacterized protein LOC111017816 [Momordica charantia])

HSP 1 Score: 94.4 bits (233), Expect = 2.02e-20
Identity = 47/65 (72.31%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           + +F  ISLCNVCYKII KAIVNR+K +L  +IS NQSAFVPGRCVVDN I+GYECVHSI
Sbjct: 152 ITEFRPISLCNVCYKIILKAIVNRMKRLLGLIISCNQSAFVPGRCVVDNAIIGYECVHSI 211

Query: 61  RNRRS 65
           R+  S
Sbjct: 212 RSDAS 216

[Close]

BLAST of MC05g_new0150 vs. NCBI nr
Match: EEC84864.1 (hypothetical protein OsI_31997 [Oryza sativa Indica Group])

HSP 1 Score: 83.6 bits (205), Expect = 9.11e-17
Identity = 41/70 (58.57%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1  MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
          + +F  ISLCNV YKI+SK +VNRL+ +LD +IS NQSAFVPGR + DN +L +EC HSI
Sbjct: 22 LKEFRPISLCNVIYKIVSKCLVNRLRPILDDLISQNQSAFVPGRMITDNALLAFECFHSI 81

Query: 61 RNRRSKSVGE 70
          +  R+KS G+
Sbjct: 82 Q--RNKSPGK 89

[Close]

BLAST of MC05g_new0150 vs. NCBI nr
Match: XP_042988708.1 (uncharacterized protein LOC122316242 [Carya illinoinensis])

HSP 1 Score: 84.0 bits (206), Expect = 9.18e-17
Identity = 40/69 (57.97%), Postives = 53/69 (76.81%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           + +F SISLCNV YK+ISK + NRLK +L+ VIS++QSAFVPGR + DNV++ YE +H +
Sbjct: 396 ITEFRSISLCNVVYKLISKVLANRLKPILNQVISSSQSAFVPGRLISDNVLVAYEVIHYL 455

Query: 61  RNRRSKSVG 69
           RNRR    G
Sbjct: 456 RNRRQGKEG 464

[Close]

BLAST of MC05g_new0150 vs. NCBI nr
Match: EAZ45328.1 (hypothetical protein OsJ_29974 [Oryza sativa Japonica Group])

HSP 1 Score: 83.6 bits (205), Expect = 1.13e-16
Identity = 41/70 (58.57%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           + +F  ISLCNV YKI+SK +VNRL+ +LD +IS NQSAFVPGR + DN +L +EC HSI
Sbjct: 184 LKEFRPISLCNVIYKIVSKCLVNRLRPILDDLISQNQSAFVPGRMITDNALLAFECFHSI 243

Query: 61  RNRRSKSVGE 70
           +  R+KS G+
Sbjct: 244 Q--RNKSPGK 251

[Close]

BLAST of MC05g_new0150 vs. NCBI nr
Match: PKA56961.1 (Putative ribonuclease H protein [Apostasia shenzhenica])

HSP 1 Score: 82.8 bits (203), Expect = 2.34e-16
Identity = 40/64 (62.50%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           M  F  ISLCNV YKII+KA+ NRLK VL S+IS+NQSAFVP R + DN+I+ YEC+HS+
Sbjct: 483 MKHFRPISLCNVTYKIIAKALANRLKPVLSSIISDNQSAFVPKRLISDNIIVAYECLHSM 542

Query: 61  RNRR 64
             R+
Sbjct: 543 NKRK 546

[Close]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL
Match: A0A6J1D5K1 (uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017816 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 9.78e-21
Identity = 47/65 (72.31%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           + +F  ISLCNVCYKII KAIVNR+K +L  +IS NQSAFVPGRCVVDN I+GYECVHSI
Sbjct: 152 ITEFRPISLCNVCYKIILKAIVNRMKRLLGLIISCNQSAFVPGRCVVDNAIIGYECVHSI 211

Query: 61  RNRRS 65
           R+  S
Sbjct: 212 RSDAS 216

[Close]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL
Match: B8BDD1 (Post-SET domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_31997 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.41e-17
Identity = 41/70 (58.57%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1  MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
          + +F  ISLCNV YKI+SK +VNRL+ +LD +IS NQSAFVPGR + DN +L +EC HSI
Sbjct: 22 LKEFRPISLCNVIYKIVSKCLVNRLRPILDDLISQNQSAFVPGRMITDNALLAFECFHSI 81

Query: 61 RNRRSKSVGE 70
          +  R+KS G+
Sbjct: 82 Q--RNKSPGK 89

[Close]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL
Match: A3C0I9 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_29974 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.46e-17
Identity = 41/70 (58.57%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1   MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
           + +F  ISLCNV YKI+SK +VNRL+ +LD +IS NQSAFVPGR + DN +L +EC HSI
Sbjct: 184 LKEFRPISLCNVIYKIVSKCLVNRLRPILDDLISQNQSAFVPGRMITDNALLAFECFHSI 243

Query: 61  RNRRSKSVGE 70
           +  R+KS G+
Sbjct: 244 Q--RNKSPGK 251

[Close]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL
Match: A0A287T920 (Reverse transcriptase domain-containing protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.06e-17
Identity = 38/63 (60.32%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 7   ISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSIRNRRSK 66
           ISLCNV YK++SK I NRLK +L  VI++NQSAFVPGR ++DN++L YE  H ++N+RS 
Sbjct: 489 ISLCNVIYKLVSKVIANRLKQILPEVIASNQSAFVPGRLIIDNILLAYEVTHFMQNKRSG 548

Query: 67  SVG 69
            VG
Sbjct: 549 EVG 551

[Close]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL
Match: A0A2N9FN47 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS16564 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 8.34e-17
Identity = 39/70 (55.71%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1    MAKFLSISLCNVCYKIISKAIVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSI 60
            MA F  ISLCNV YKIISK +VNR+K++L  VIS +QSAFVPGR + DN+++ +E +H +
Sbjct: 939  MAHFRPISLCNVIYKIISKVLVNRMKNLLSDVISYSQSAFVPGRMITDNILVSFEMLHYL 998

Query: 61   RNRRSKSVGE 70
            +N+R   VG+
Sbjct: 999  KNKRGGKVGQ 1008

[Close]

BLAST of MC05g_new0150 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 42.4 bits (98), Expect = 1.9e-04
Identity = 17/44 (38.64%), Postives = 30/44 (68.18%), Query Frame = 0


Query: 21 IVNRLKDVLDSVISNNQSAFVPGRCVVDNVILGYECVHSIRNRR 65
          +V RLK ++ ++I   Q++F+PGR   DN++   E VHS+R ++
Sbjct: 1  MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKK 44

The following BLAST results are available for this feature:

BLAST of MC05g_new0150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14381	3.6e-05	49.09	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]

BLAST of MC05g_new0150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149385.1	2.02e-20	72.31	uncharacterized protein LOC111017816 [Momordica charantia]	[more]
EEC84864.1	9.11e-17	58.57	hypothetical protein OsI_31997 [Oryza sativa Indica Group]	[more]
XP_042988708.1	9.18e-17	57.97	uncharacterized protein LOC122316242 [Carya illinoinensis]	[more]
EAZ45328.1	1.13e-16	58.57	hypothetical protein OsJ_29974 [Oryza sativa Japonica Group]	[more]
PKA56961.1	2.34e-16	62.50	Putative ribonuclease H protein [Apostasia shenzhenica]	[more]

BLAST of MC05g_new0150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5K1	9.78e-21	72.31	uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
B8BDD1	4.41e-17	58.57	Post-SET domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI...	[more]
A3C0I9	5.46e-17	58.57	Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica ...	[more]
A0A287T920	6.06e-17	60.32	Reverse transcriptase domain-containing protein OS=Hordeum vulgare subsp. vulgar...	[more]
A0A2N9FN47	8.34e-17	55.71	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS16564 PE=4 SV=1	[more]

BLAST of MC05g_new0150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20520.1	1.9e-04	38.64	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 7..66 e-value: 4.8E-11 score: 42.6
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 4..65
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 4..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0150.1	MC05g_new0150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity