MC05g_new0127 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0127
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	calmodulin-like protein 3
Location	MC05: 6925478 .. 6927345 (+)
RNA-Seq Expression	MC05g_new0127
Synteny	MC05g_new0127

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAATCAAGTAAGTTTCTAAATTAATCGACAAAGCCACCCACCAAACCAAACGATAATAATGAGTACGCGTTTTTGGCAAAAAAGCGTAAAAAAAGAAAAAAGAAAAAAAGAAATTGTATTTTCCTTTTTTTAGAAAGAAAAAGAAAAATCCCCCAATTACGCGTGAAGGAATCGCAATTTGCAACCCCTTGAAAAAGGGCTCCGCCATTGAAACCGTTGGTTCATCTTCCTCCCTCACCTCTCTGTGTAAACCCGATTTTCTCTCCCGAATTTTCTCGGCAACCAAACAGGCTCCACCACATACATTTCCGTTTCCCCCATTTTTTCCTTCGGATGCCAGCCATTTTCCCCAGGATTTTCCGGTTTTCACAATCTCCCCTGCCGCATTAATTCCTCCTCTGTTCGGTTTCCGAGAAAGAAAGAAAAAGAAAAAAACAGAGAGAAGAAACAATAATCCTCCATTCCCTTCCCCTTCCTCTCTCCCCTTCCTCTCTACGCATCATCATCATCCAGTCGCCGGCCGATCTCCACCACCGACTCTTCACGCATCTCGAATTCGATCGATCGATGGACCCGACGGAACTGAGAAGAGTGTTCCAGATGTTCGATCGCAACGGCGACGGGCGGATCACGAAGAAGGAATTAAGCGATTCGTTGGAGAATTTGGGGATCTTTATCCCGGACAAAGATCTGACTCAAATGATCGAGAAAATCGACGTGAACTGTGACGGATGCGTGGACATTGACGAGTTCGGGGAGCTGTACCAGTCGATTATGGACGAGCGCGACGAGGAGGAAGACATGAGAGAAGCCTTCAACGTGTTCGACCAGAACGGAGACGGATTCATCACCGTCGAGGAATTGAGGTCAGTATTAGCTTCCTTGGGGCTTAAGCAAGGTAGAACTGTAGAGGATTGCAAGAAGATGATCATGAAGGTGGATGTGGATGGAGATGGAATGGTGAATTACAAAGAGTTCAAGCAAATGATGAAGGGAGGTGGGTTTAGTGCATTAGGTTGATCTTTTCTCGCTTAATCACACACACACTTCACACTTCACACATTCGCACAGACAGAGGTGAGAGGAAGGAGATCAAGAAACTGAAAATTCCAAAAAGAAAAAAAACCAAAAAAAAGGTAAGAGAGAGGATTGTTTTTCCATTAGAGTCTGTACATAAAACCTTGGAGATTGAAGATGATGATGAAGAAGAAGGAGAAGAAGGAGAAGAAGGAAGGAGAAAGATGGGTCATTACTACACAGGTTGCATGGATTATTTGTGCACTCAATCACACGTGTCGATGTGGCATTGGTTGGAATTGGAGCCAATGAGCTTTTCACACGTATTGGAATTTATATATTATACAAATGTATTTTTCATTTTTTTTTAATTGAAATTATGATCATGATGATGTAGGTGAAAAATTGAATGAATAGTCCATGTAATTTTGAAATTTGTCGTTGTTGGGGAAGAAGGAAGAAGAAAGGCATAGTGAAACATTTGTGGGTTCTCGGGTCGGGTCACCAACAGATTATTATTGTGCTTCTTCTTTTCTTTTTTTCTCTCTCTTTTTTTCTTTTTTTTTTTTTTAATTGCATTCCAACTCAGTAAGATTGTTTTTGTGTTTCTTTCCCTCTTTTAATGAATTTGTTTTGTTCATGTCTTCCTTTCCATATTGCTCTCTTTTTTGGGACAATTAGAGGTTATGTTTGATTGTAATTTAGTTCTTAAAAAAATAATTTTGTGGGAAGTTTTGGAAGATAATAATTAACAGAAATTGAATTTTTTTTTTCTTTTAGTAAAATCTGCATCTATCTCAATTAGTTGTTGAGGTATTATTTCATATCAAAAGTCATTAGGTACGAAG

mRNA sequence

ATGGACCCGACGGAACTGAGAAGAGTGTTCCAGATGTTCGATCGCAACGGCGACGGGCGGATCACGAAGAAGGAATTAAGCGATTCGTTGGAGAATTTGGGGATCTTTATCCCGGACAAAGATCTGACTCAAATGATCGAGAAAATCGACGTGAACTGTGACGGATGCGTGGACATTGACGAGTTCGGGGAGCTGTACCAGTCGATTATGGACGAGCGCGACGAGGAGGAAGACATGAGAGAAGCCTTCAACGTGTTCGACCAGAACGGAGACGGATTCATCACCGTCGAGGAATTGAGGTCAGTATTAGCTTCCTTGGGGCTTAAGCAAGGTAGAACTGTAGAGGATTGCAAGAAGATGATCATGAAGGTGGATGTGGATGGAGATGGAATGGTGAATTACAAAGAGTTCAAGCAAATGATGAAGGGAGGTGGGTTTAGTGCATTAGGTTGA

Coding sequence (CDS)

ATGGACCCGACGGAACTGAGAAGAGTGTTCCAGATGTTCGATCGCAACGGCGACGGGCGGATCACGAAGAAGGAATTAAGCGATTCGTTGGAGAATTTGGGGATCTTTATCCCGGACAAAGATCTGACTCAAATGATCGAGAAAATCGACGTGAACTGTGACGGATGCGTGGACATTGACGAGTTCGGGGAGCTGTACCAGTCGATTATGGACGAGCGCGACGAGGAGGAAGACATGAGAGAAGCCTTCAACGTGTTCGACCAGAACGGAGACGGATTCATCACCGTCGAGGAATTGAGGTCAGTATTAGCTTCCTTGGGGCTTAAGCAAGGTAGAACTGTAGAGGATTGCAAGAAGATGATCATGAAGGTGGATGTGGATGGAGATGGAATGGTGAATTACAAAGAGTTCAAGCAAATGATGAAGGGAGGTGGGTTTAGTGCATTAGGTTGA

Protein sequence

MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDIDEFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKMIMKVDVDGDGMVNYKEFKQMMKGGGFSALG

Homology

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BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.2e-68
Identity = 128/150 (85.33%), Postives = 141/150 (94.00%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MD  EL R+FQMFDRNGDG+ITK+EL+DSLENLGI+IPDKDL QMIEKID+N DG VDI+
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFG LYQ+IM+ERDEEEDMREAFNVFDQN DGFITVEELRSVLASLGLKQGRT+EDCK+M
Sbjct: 61  EFGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 151
           I KVDVDGDGMVN+KEFKQMMKGGGF+ALG
Sbjct: 121 ISKVDVDGDGMVNFKEFKQMMKGGGFAALG 150

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BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 4.5e-66
Identity = 122/150 (81.33%), Postives = 142/150 (94.67%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTEL+RVFQMFD+NGDG IT KELS++L +LGI+IPDK+LTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERD-EEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKK 120
           EFGELY++IMDE D EEEDM+EAFNVFDQNGDGFITV+EL++VL+SLGLKQG+T++DCKK
Sbjct: 61  EFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKK 120

Query: 121 MIMKVDVDGDGMVNYKEFKQMMKGGGFSAL 150
           MI KVDVDGDG VNYKEF+QMMKGGGF++L
Sbjct: 121 MIKKVDVDGDGRVNYKEFRQMMKGGGFNSL 150

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BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 1.8e-62
Identity = 118/153 (77.12%), Postives = 136/153 (88.89%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           +DP+EL+RVFQMFD+NGDGRITK+EL+DSLENLGI+IPDKDLTQMI KID N DGCVDID
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 61  EFGELYQSIMDE-----RDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVE 120
           EF  LY SI+DE       EEEDM++AFNVFDQ+GDGFITVEEL+SV+ASLGLKQG+T++
Sbjct: 121 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 180

Query: 121 DCKKMIMKVDVDGDGMVNYKEFKQMMKGGGFSA 149
            CKKMIM+VD DGDG VNYKEF QMMKGGGFS+
Sbjct: 181 GCKKMIMQVDADGDGRVNYKEFLQMMKGGGFSS 213

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BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.2e-58
Identity = 108/149 (72.48%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MD  EL RVFQMFD+NGDG+I K EL D  +++GI +P+ ++ +MI K+DVN DG +DID
Sbjct: 1   MDRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFG LYQ +++E++EEEDMREAF VFDQNGDGFIT EELRSVLAS+GLKQGRT+EDCKKM
Sbjct: 61  EFGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSAL 150
           I KVDVDGDGMVN+KEFKQMM+GGGF+AL
Sbjct: 121 ISKVDVDGDGMVNFKEFKQMMRGGGFAAL 149

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BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot
Match: Q9LX27 (Calmodulin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CML4 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.1e-56
Identity = 109/145 (75.17%), Postives = 128/145 (88.28%), Query Frame = 0


Query: 3   PTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDIDEF 62
           P +L+RVFQMFD+NGDGRITK+EL+DSLENLGIF+PDKDL QMI+K+D N DGCVDI+EF
Sbjct: 49  PVDLKRVFQMFDKNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEF 108

Query: 63  GELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKMIM 122
             LY SI++E+ EE DMR+AFNVFDQ+GDGFITVEEL SV+ SLGLKQG+T+E CK+MIM
Sbjct: 109 ESLYGSIVEEK-EEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIM 168

Query: 123 KVDVDGDGMVNYKEFKQMMKGGGFS 148
           +VD DGDG VNYKEF QMMK G FS
Sbjct: 169 QVDEDGDGRVNYKEFLQMMKSGDFS 192

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BLAST of MC05g_new0127 vs. NCBI nr
Match: XP_022145111.1 (calmodulin-like protein 3 [Momordica charantia])

HSP 1 Score: 300 bits (767), Expect = 1.83e-102
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

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BLAST of MC05g_new0127 vs. NCBI nr
Match: XP_004152098.1 (calmodulin-like protein 3 [Cucumis sativus] >XP_008453977.1 PREDICTED: calmodulin-like protein 3 [Cucumis melo] >XP_038878708.1 calmodulin-like protein 3 [Benincasa hispida] >KAE8649113.1 hypothetical protein Csa_015095 [Cucumis sativus] >BAI52955.1 calcium-binding EF-hand protein [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 294 bits (752), Expect = 3.56e-100
Identity = 148/150 (98.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. NCBI nr
Match: XP_022979973.1 (calmodulin-like protein 3 [Cucurbita maxima])

HSP 1 Score: 290 bits (742), Expect = 1.19e-98
Identity = 145/150 (96.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTEL+RVFQMFDRNGDGRIT+KELSDSLENLGIFIPDKDLTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELKRVFQMFDRNGDGRITQKELSDSLENLGIFIPDKDLTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDE+DEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDEKDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. NCBI nr
Match: XP_023526445.1 (calmodulin-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288 bits (737), Expect = 7.14e-98
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTEL+RVFQMFDRNGDGRIT+KELSDSLENLGIFIPDKDLT MIEKIDVN DGCVDID
Sbjct: 1   MDPTELKRVFQMFDRNGDGRITQKELSDSLENLGIFIPDKDLTHMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDE+DEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDEKDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. NCBI nr
Match: XP_022932721.1 (calmodulin-like protein 3 [Cucurbita moschata] >KAG6597646.1 Calmodulin-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029087.1 Calmodulin-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 286 bits (733), Expect = 2.81e-97
Identity = 145/150 (96.67%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKEL+DSLENLGIFIPDKDLTQMIEKIDVN DG VDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELNDSLENLGIFIPDKDLTQMIEKIDVNNDGYVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIM+E+DEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMNEKDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL
Match: A0A6J1CVD5 (calmodulin-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111014628 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 8.88e-103
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL
Match: D1MWZ5 (Calcium-binding EF-hand protein OS=Citrullus lanatus subsp. vulgaris OX=260674 GN=CitCaEFh PE=2 SV=1)

HSP 1 Score: 294 bits (752), Expect = 1.72e-100
Identity = 148/150 (98.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL
Match: A0A1S3BXJ7 (calmodulin-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103494537 PE=4 SV=1)

HSP 1 Score: 294 bits (752), Expect = 1.72e-100
Identity = 148/150 (98.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL
Match: A0A6J1IXV3 (calmodulin-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111479505 PE=4 SV=1)

HSP 1 Score: 290 bits (742), Expect = 5.77e-99
Identity = 145/150 (96.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTEL+RVFQMFDRNGDGRIT+KELSDSLENLGIFIPDKDLTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELKRVFQMFDRNGDGRITQKELSDSLENLGIFIPDKDLTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIMDE+DEEEDMREAFNVFDQNGDGFITV+ELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMDEKDEEEDMREAFNVFDQNGDGFITVDELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL
Match: A0A6J1EX62 (calmodulin-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111439186 PE=4 SV=1)

HSP 1 Score: 286 bits (733), Expect = 1.36e-97
Identity = 145/150 (96.67%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTELRRVFQMFDRNGDGRITKKEL+DSLENLGIFIPDKDLTQMIEKIDVN DG VDID
Sbjct: 1   MDPTELRRVFQMFDRNGDGRITKKELNDSLENLGIFIPDKDLTQMIEKIDVNNDGYVDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFGELYQSIM+E+DEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM
Sbjct: 61  EFGELYQSIMNEKDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150
           IMKVDVDGDGMVNYKEFKQMMKGGGFSALG
Sbjct: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 150

[Close]

BLAST of MC05g_new0127 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 259.6 bits (662), Expect = 1.5e-69
Identity = 128/150 (85.33%), Postives = 141/150 (94.00%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MD  EL R+FQMFDRNGDG+ITK+EL+DSLENLGI+IPDKDL QMIEKID+N DG VDI+
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFG LYQ+IM+ERDEEEDMREAFNVFDQN DGFITVEELRSVLASLGLKQGRT+EDCK+M
Sbjct: 61  EFGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSALG 151
           I KVDVDGDGMVN+KEFKQMMKGGGF+ALG
Sbjct: 121 ISKVDVDGDGMVNFKEFKQMMKGGGFAALG 150

[Close]

BLAST of MC05g_new0127 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 251.9 bits (642), Expect = 3.2e-67
Identity = 122/150 (81.33%), Postives = 142/150 (94.67%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MDPTEL+RVFQMFD+NGDG IT KELS++L +LGI+IPDK+LTQMIEKIDVN DGCVDID
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 61  EFGELYQSIMDERD-EEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKK 120
           EFGELY++IMDE D EEEDM+EAFNVFDQNGDGFITV+EL++VL+SLGLKQG+T++DCKK
Sbjct: 61  EFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKK 120

Query: 121 MIMKVDVDGDGMVNYKEFKQMMKGGGFSAL 150
           MI KVDVDGDG VNYKEF+QMMKGGGF++L
Sbjct: 121 MIKKVDVDGDGRVNYKEFRQMMKGGGFNSL 150

[Close]

BLAST of MC05g_new0127 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 240.0 bits (611), Expect = 1.3e-63
Identity = 118/153 (77.12%), Postives = 136/153 (88.89%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           +DP+EL+RVFQMFD+NGDGRITK+EL+DSLENLGI+IPDKDLTQMI KID N DGCVDID
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 61  EFGELYQSIMDE-----RDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVE 120
           EF  LY SI+DE       EEEDM++AFNVFDQ+GDGFITVEEL+SV+ASLGLKQG+T++
Sbjct: 121 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 180

Query: 121 DCKKMIMKVDVDGDGMVNYKEFKQMMKGGGFSA 149
            CKKMIM+VD DGDG VNYKEF QMMKGGGFS+
Sbjct: 181 GCKKMIMQVDADGDGRVNYKEFLQMMKGGGFSS 213

[Close]

BLAST of MC05g_new0127 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 227.3 bits (578), Expect = 8.4e-60
Identity = 108/149 (72.48%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 1   MDPTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDID 60
           MD  EL RVFQMFD+NGDG+I K EL D  +++GI +P+ ++ +MI K+DVN DG +DID
Sbjct: 1   MDRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDID 60

Query: 61  EFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKM 120
           EFG LYQ +++E++EEEDMREAF VFDQNGDGFIT EELRSVLAS+GLKQGRT+EDCKKM
Sbjct: 61  EFGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKM 120

Query: 121 IMKVDVDGDGMVNYKEFKQMMKGGGFSAL 150
           I KVDVDGDGMVN+KEFKQMM+GGGF+AL
Sbjct: 121 ISKVDVDGDGMVNFKEFKQMMRGGGFAAL 149

[Close]

BLAST of MC05g_new0127 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 220.7 bits (561), Expect = 7.9e-58
Identity = 109/145 (75.17%), Postives = 128/145 (88.28%), Query Frame = 0


Query: 3   PTELRRVFQMFDRNGDGRITKKELSDSLENLGIFIPDKDLTQMIEKIDVNCDGCVDIDEF 62
           P +L+RVFQMFD+NGDGRITK+EL+DSLENLGIF+PDKDL QMI+K+D N DGCVDI+EF
Sbjct: 49  PVDLKRVFQMFDKNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEF 108

Query: 63  GELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRSVLASLGLKQGRTVEDCKKMIM 122
             LY SI++E+ EE DMR+AFNVFDQ+GDGFITVEEL SV+ SLGLKQG+T+E CK+MIM
Sbjct: 109 ESLYGSIVEEK-EEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIM 168

Query: 123 KVDVDGDGMVNYKEFKQMMKGGGFS 148
           +VD DGDG VNYKEF QMMK G FS
Sbjct: 169 QVDEDGDGRVNYKEFLQMMKSGDFS 192

The following BLAST results are available for this feature:

BLAST of MC05g_new0127 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRR7	2.2e-68	85.33	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	4.5e-66	81.33	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
O22845	1.8e-62	77.12	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9SU00	1.2e-58	72.48	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9LX27	1.1e-56	75.17	Calmodulin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=CML4 PE=2 SV=1	[more]

BLAST of MC05g_new0127 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145111.1	1.83e-102	100.00	calmodulin-like protein 3 [Momordica charantia]	[more]
XP_004152098.1	3.56e-100	98.67	calmodulin-like protein 3 [Cucumis sativus] >XP_008453977.1 PREDICTED: calmoduli...	[more]
XP_022979973.1	1.19e-98	96.67	calmodulin-like protein 3 [Cucurbita maxima]	[more]
XP_023526445.1	7.14e-98	96.00	calmodulin-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022932721.1	2.81e-97	96.67	calmodulin-like protein 3 [Cucurbita moschata] >KAG6597646.1 Calmodulin-like pro...	[more]

BLAST of MC05g_new0127 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVD5	8.88e-103	100.00	calmodulin-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111014628 PE=4 SV...	[more]
D1MWZ5	1.72e-100	98.67	Calcium-binding EF-hand protein OS=Citrullus lanatus subsp. vulgaris OX=260674 G...	[more]
A0A1S3BXJ7	1.72e-100	98.67	calmodulin-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103494537 PE=4 SV=1	[more]
A0A6J1IXV3	5.77e-99	96.67	calmodulin-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111479505 PE=4 SV=1	[more]
A0A6J1EX62	1.36e-97	96.67	calmodulin-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111439186 PE=4 SV=...	[more]

BLAST of MC05g_new0127 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07490.1	1.5e-69	85.33	ARF-GAP domain 11	[more]
AT1G05990.1	3.2e-67	81.33	EF hand calcium-binding protein family	[more]
AT2G43290.1	1.3e-63	77.12	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	8.4e-60	72.48	EF hand calcium-binding protein family	[more]
AT3G59440.1	7.9e-58	75.17	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 41..69 e-value: 0.011 score: 24.9 coord: 116..144 e-value: 4.5E-5 score: 32.9 coord: 78..106 e-value: 4.2E-7 score: 39.6 coord: 5..33 e-value: 5.8E-5 score: 32.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 76..142 e-value: 1.2E-13 score: 51.3 coord: 5..67 e-value: 3.1E-13 score: 50.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 17.358736
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..36 score: 14.597092
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 112..147 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 37..72 score: 12.142296
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 5..62 e-value: 1.1693E-13 score: 59.8689
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 82..142 e-value: 2.90835E-16 score: 66.4173
None	No IPR available	GENE3D	1.10.238.10		coord: 2..68 e-value: 2.2E-18 score: 68.1
None	No IPR available	GENE3D	1.10.238.10		coord: 69..144 e-value: 9.3E-25 score: 88.8
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..149
None	No IPR available	PANTHER	PTHR10891:SF901	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 1..149
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 50..62
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 14..26
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0127.1	MC05g_new0127.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding