MC05g_new0121 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0121
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	15-cis-zeta-carotene isomerase, chloroplastic
Location	MC05: 6854584 .. 6857420 (+)
RNA-Seq Expression	MC05g_new0121
Synteny	MC05g_new0121

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTGAACTAATAATTTTTAAAGTAGCCAAAGTGAATATAGTTATTAACATGTATCTCCAACCAAAAAGTTGTGGGTTTTTCGATTCTACATCCAAAGTAAGGTACATAACCTCTTGATCAGAGATATATGCTAATTATCAATGAATTATCCTCGCTTTGGCACATAATCAGTCCTATTTCTTTTCAATGGCAATATTTCTTTGATAAAATATTAATTAGTTAGTTGTCGTAAACACTTTCTTTCTCCCCCACCAACTTAGCTAATATCCTCTGTGAATGCGTCTCCAATTGCATAGATACATAATTCAATAAAATCCAAAACCTTTCCCAAAGCGCACCAATTACTTCAGATTCTCCTTCTCATTGCCTTACAACTTACAACCACCGCGGGTTTTCCAATGGCGGTAGCCTCCGCCGCCGCCTCCGCCGCGCCCCTTCTTCACTACCCACTCGCAGCCTCCTCCTCCAACCAACCCCACCGTCGCCATCGCCGCCCGGATGCTATTAGCCTCCGCCACTCTTCAATTTCGATCCCCAATTTCCCTCCGTATCCCCTCAACTGCTCGCCGATTTCCGGACCCCGTCGGCTTGCTACCCGTTTGGGGGATTTTTCTGCCGGCGCTTCCATCGGAGACAGCGATACTGGTGGTTCGACCTCTGTCTCGGACGACGACGGCCTCGTCGGCGAGGACGCTGCGGTTTTTGATCTTTCCAAGCAGAAATTGACGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTTTGTTCGTGCTCAATGTTGTTTGGATTGATAACTCCGGTGTGGGATTTGGCAAGGCCTTTGTGGACGCTGTTTCTGAACTCTCGGATAGTCATGAGGTTCCCTTCTATTACTTCGATTTCTTTAAATTGTTTCTGGATTGGTATATTTTTTTTTTTACCTGTACAAACAATGCTCATTGTGAATGTGATTCTGTATAACGTTCCCCAAAATGGGAGAGATTATCTTACTCTCAGATAGTTTTTACTGGTTGGTTTTGGTTGAAGCGATTGGATGTCTATAGCTAGTGGTTGGGCTTCCAATTTCATTCCTAATCTGAAGTTTTAGCTTCGTTTCTTTCAAGAAATCCTTCCTGTTAGTGAACTTAAAATCAATGATAGATGTTGAAATTTGAAAATTGTGTTCATTAGTACCATTTTAAATGGGTTTGTTTTCTAATTTTGGGCCATGTGAATTAAATAGATTTGTCAATTTTATGGTAGGCATGTCTAGCTCGGGATAGTTTCTACTGGATTAATGAAAGGAATTTTGAAGTTTAGTGAACTTAATATGTAAAGGGTGGAATTCAAACTTTAAAAGATTATGGACGAAAAATCTTACATGTCTTTGGCTTCTGAATTATGAATGACTGGGAGGATGAACTTAGCATTTGGTTTATATTGTGCATGGCCTTGCTAAATTCCTGAGTCTCACTTGGTTAACATGAATCCAAGTCTTGACTCTTGAAACTTTCTTCTGCAGGTTGTGATGTTGATGCTCATTTCCATTTTTGCCATTGCCCACAGTGGTTTGGCCACTCTTAGAGACCAAGGCGAGAAGCTTGTCGGTGAACGAGCTTTTCGGGTTCTGTTTGCAGGAATTTCTCTACCTTTGGCTGTCAGCACTGTGGTAGGATACCTTCCCTCTTCTTTTCCATTTAACTTGATGATGAAGTTATCTTAATATATATTGTTCCTTAAAAAATTCTGGATGCACACCCATATTGACTTGTTTACGCTAAGGAGTTGAATTCAGGTGTATTTCATTAACCATCGATACGACGGAGTACAATTATGGCAGCTCCAAAGTGTTCCCGGAGTGCATCAGCTCGTGTGGTTCAGTTCATTTATCTCCTTCTTCTTCCTCTATCCTTCAACTTTTAATTTGCTAGAGGTTGCAGCTGTTGATAAACCGAAAATGCACCTTTGGGAAACCGGCATCATTAGAATAACTAGGCATCCACAGGTATGTTTATATTTCCTTAACTTGAACATTCATTACATTTCATTACAATTTCGGGGTTTCATTGCCGTTAATTTGGCTGATAAACAAGGCTTAGGCTCAAATCTCGAACTAGAATTGGTGGCTTCGGCTTGAATCCATTGATAAACTAGAGTTGTCTCATGTTACTTTACTGCATCAAGAATTTTCAATGAACTTTCATACTTTTTATTTTAGTCCCAAAACTTTCAAAAGTTAAAAAGGCTTCTCAGCAAATCTCGGTTTCTCTTCAAAGATGGTTGGGCAGGTGATGTGGTGCTTGGCTCATACCATCTGGATAGGGAACTCTGTTGCAGTGGCAGCTTCCATTGGCTTGATAGGACATCATCTGTTCGGGGTATGGAACGGAGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCCGTGAAAAGGCGAACGAGCGTCATCCCGTTCGCTGCCATTGTGGACGGGCGTCAAAAGTTGCCCGACGATTATTACAAGGAGTTCATTCGGTTGCCATATTTGTCAATAACTGCACTGACAATGGGTGCGTACCTTGCTCACCCCCTTATGCAAGCTGCAAGTTTCAGGCTTCATTGGTAAGTATATGCAGCTAACTCTTTTGTATCAGATGTATAATTATTGTATAAGCCAAAGCATAGAGTGACACTGGATAGAGAAATTTGAGCTCATGGACTTATTAATTAAATATTTACAAATATAGGAGCTATTTATATATATATATAGCTCAAAGTGGGACTTTTGTAATTTTATATTAATCGAATAAACGAAAATAAATCATTTGTATCAGTTATTTTGCATATCATTAAACAACACTAATTCTCA

mRNA sequence

ATGGCGGTAGCCTCCGCCGCCGCCTCCGCCGCGCCCCTTCTTCACTACCCACTCGCAGCCTCCTCCTCCAACCAACCCCACCGTCGCCATCGCCGCCCGGATGCTATTAGCCTCCGCCACTCTTCAATTTCGATCCCCAATTTCCCTCCGTATCCCCTCAACTGCTCGCCGATTTCCGGACCCCGTCGGCTTGCTACCCGTTTGGGGGATTTTTCTGCCGGCGCTTCCATCGGAGACAGCGATACTGGTGGTTCGACCTCTGTCTCGGACGACGACGGCCTCGTCGGCGAGGACGCTGCGGTTTTTGATCTTTCCAAGCAGAAATTGACGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTTTGTTCGTGCTCAATGTTGTTTGGATTGATAACTCCGGTGTGGGATTTGGCAAGGCCTTTGTGGACGCTGTTTCTGAACTCTCGGATAGTCATGAGGTTGTGATGTTGATGCTCATTTCCATTTTTGCCATTGCCCACAGTGGTTTGGCCACTCTTAGAGACCAAGGCGAGAAGCTTGTCGGTGAACGAGCTTTTCGGGTTCTGTTTGCAGGAATTTCTCTACCTTTGGCTGTCAGCACTGTGGTGTATTTCATTAACCATCGATACGACGGAGTACAATTATGGCAGCTCCAAAGTGTTCCCGGAGTGCATCAGCTCGTGTGGTTCAGTTCATTTATCTCCTTCTTCTTCCTCTATCCTTCAACTTTTAATTTGCTAGAGGTTGCAGCTGTTGATAAACCGAAAATGCACCTTTGGGAAACCGGCATCATTAGAATAACTAGGCATCCACAGATGGTTGGGCAGGTGATGTGGTGCTTGGCTCATACCATCTGGATAGGGAACTCTGTTGCAGTGGCAGCTTCCATTGGCTTGATAGGACATCATCTGTTCGGGGTATGGAACGGAGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCCGTGAAAAGGCGAACGAGCGTCATCCCGTTCGCTGCCATTGTGGACGGGCGTCAAAAGTTGCCCGACGATTATTACAAGGAGTTCATTCGGTTGCCATATTTGTCAATAACTGCACTGACAATGGGTGCGTACCTTGCTCACCCCCTTATGCAAGCTGCAAGTTTCAGGCTTCATTGGTAA

Coding sequence (CDS)

ATGGCGGTAGCCTCCGCCGCCGCCTCCGCCGCGCCCCTTCTTCACTACCCACTCGCAGCCTCCTCCTCCAACCAACCCCACCGTCGCCATCGCCGCCCGGATGCTATTAGCCTCCGCCACTCTTCAATTTCGATCCCCAATTTCCCTCCGTATCCCCTCAACTGCTCGCCGATTTCCGGACCCCGTCGGCTTGCTACCCGTTTGGGGGATTTTTCTGCCGGCGCTTCCATCGGAGACAGCGATACTGGTGGTTCGACCTCTGTCTCGGACGACGACGGCCTCGTCGGCGAGGACGCTGCGGTTTTTGATCTTTCCAAGCAGAAATTGACGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTTTGTTCGTGCTCAATGTTGTTTGGATTGATAACTCCGGTGTGGGATTTGGCAAGGCCTTTGTGGACGCTGTTTCTGAACTCTCGGATAGTCATGAGGTTGTGATGTTGATGCTCATTTCCATTTTTGCCATTGCCCACAGTGGTTTGGCCACTCTTAGAGACCAAGGCGAGAAGCTTGTCGGTGAACGAGCTTTTCGGGTTCTGTTTGCAGGAATTTCTCTACCTTTGGCTGTCAGCACTGTGGTGTATTTCATTAACCATCGATACGACGGAGTACAATTATGGCAGCTCCAAAGTGTTCCCGGAGTGCATCAGCTCGTGTGGTTCAGTTCATTTATCTCCTTCTTCTTCCTCTATCCTTCAACTTTTAATTTGCTAGAGGTTGCAGCTGTTGATAAACCGAAAATGCACCTTTGGGAAACCGGCATCATTAGAATAACTAGGCATCCACAGATGGTTGGGCAGGTGATGTGGTGCTTGGCTCATACCATCTGGATAGGGAACTCTGTTGCAGTGGCAGCTTCCATTGGCTTGATAGGACATCATCTGTTCGGGGTATGGAACGGAGACAGGAGGCTAGCAAAGCGATACGGGGCAGATTTTGAAGCCGTGAAAAGGCGAACGAGCGTCATCCCGTTCGCTGCCATTGTGGACGGGCGTCAAAAGTTGCCCGACGATTATTACAAGGAGTTCATTCGGTTGCCATATTTGTCAATAACTGCACTGACAATGGGTGCGTACCTTGCTCACCCCCTTATGCAAGCTGCAAGTTTCAGGCTTCATTGGTAA

Protein sequence

MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISGPRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALTMGAYLAHPLMQAASFRLHW

Homology

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BLAST of MC05g_new0121 vs. ExPASy Swiss-Prot
Match: Q9SAC0 (15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=Z-ISO PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 1.6e-139
Identity = 236/300 (78.67%), Postives = 268/300 (89.33%), Query Frame = 0


Query: 85  STSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVWIDNSGVGFGKAFVD 144
           S S S    L+GED+A F+L KQKL SW YF V+LGVVLF+LNVVWIDNS  GFGK+F+D
Sbjct: 69  SDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNS-TGFGKSFID 128

Query: 145 AVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVLFAGISLPLAVSTVV 204
           AVS +S S EV MLMLI IFAI HSGLA+LRD GEKL+GERAFRVLFAGISLPLA+ST+V
Sbjct: 129 AVSNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIV 188

Query: 205 YFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETG 264
           YFINHRYDG QLWQLQ VPGVH+ +W ++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETG
Sbjct: 189 YFINHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETG 248

Query: 265 IIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFE 324
           I+RITRHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE
Sbjct: 249 IMRITRHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFE 308

Query: 325 AVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALTMGAYLAHPLMQAASFRLHW 384
           ++K+RTSVIPFAAI +GRQ LP+DYYKEF+RLPYL+ITALT+GAY AHPLMQ ASFRLHW
Sbjct: 309 SIKKRTSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

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BLAST of MC05g_new0121 vs. ExPASy Swiss-Prot
Match: B4FHU1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.5e-129
Identity = 235/374 (62.83%), Postives = 279/374 (74.60%), Query Frame = 0


Query: 11  APLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISGPRRLATRLGD 70
           A  L   LAA+    PHRR      +    + I  P  PP     S     R +   +  
Sbjct: 2   ASQLRLHLAATPPLLPHRRPHLARPLCPTLNPIRAP-LPPLSRVLSHARPARAVGGGIEP 61

Query: 71  FSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVW 130
                + GD   GG         LVGED+A F+L  Q + SWAYF  ILG VL  LNV+W
Sbjct: 62  KEGVVAEGDESGGGPV-------LVGEDSAAFELKDQSVASWAYFAGILGAVLVALNVLW 121

Query: 131 IDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVL 190
           ID S  G G  F+DAV+ +SDSHEVVML+L  IFA+ HSG+A+LR+ GEK+VGER +RVL
Sbjct: 122 IDPS-TGVGTKFLDAVASVSDSHEVVMLLLTIIFAVVHSGMASLRESGEKIVGERVYRVL 181

Query: 191 FAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEV 250
           FAGISLPLAV+T+VYFINHRYDG QLWQ+Q + G+H+L+WFSSFISFFFLYPSTFNLLEV
Sbjct: 182 FAGISLPLAVTTIVYFINHRYDGTQLWQVQGITGIHELLWFSSFISFFFLYPSTFNLLEV 241

Query: 251 AAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVWN 310
           AAVDKPK+H+WETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAAS+GLI HHLFG WN
Sbjct: 242 AAVDKPKLHMWETGIMRITRHPQMVGQVIWCLAHTLWIGNSVAVAASVGLISHHLFGAWN 301

Query: 311 GDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALTMGAYL 370
           GDRRL  RYG  FE +K+RTSV+PFAAI+DGRQKLP DY+KEF RLPY++IT LT+GAY 
Sbjct: 302 GDRRLLSRYGEAFEVLKKRTSVMPFAAIIDGRQKLPKDYHKEFFRLPYVAITMLTLGAYF 361

Query: 371 AHPLMQAASFRLHW 385
           AHPLMQA+S++L W
Sbjct: 362 AHPLMQASSYQLPW 366

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BLAST of MC05g_new0121 vs. NCBI nr
Match: XP_022145219.1 (15-cis-zeta-carotene isomerase, chloroplastic [Momordica charantia])

HSP 1 Score: 757 bits (1954), Expect = 1.37e-275
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG 60
           MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG
Sbjct: 1   MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG 60

Query: 61  PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG 120
           PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG
Sbjct: 61  PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG 120

Query: 121 VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK 180
           VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK
Sbjct: 121 VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK 180

Query: 181 LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL 240
           LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL
Sbjct: 181 LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL 240

Query: 241 YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL 300
           YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL
Sbjct: 241 YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL 300

Query: 301 IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS 360
           IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS
Sbjct: 301 IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS 360

Query: 361 ITALTMGAYLAHPLMQAASFRLHW 384
           ITALTMGAYLAHPLMQAASFRLHW
Sbjct: 361 ITALTMGAYLAHPLMQAASFRLHW 384

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BLAST of MC05g_new0121 vs. NCBI nr
Match: XP_022972107.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 623 bits (1607), Expect = 7.28e-223
Identity = 322/382 (84.29%), Postives = 340/382 (89.01%), Query Frame = 0


Query: 6   AAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPP---YPLNCSPISGPR 65
           A ASA  L   P   +SSNQPH RHRRPDA +LR SSIS PNF P   + LN SPI   R
Sbjct: 2   AVASATTLFFSPFP-TSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 61

Query: 66  RLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVV 125
           R       FSA ASIGDS++GGSTS SDDDGLVGEDAAVF+LS+QKL SW YFTVILGVV
Sbjct: 62  R-------FSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVV 121

Query: 126 LFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLV 185
           LFVLNVVWIDNS  G GKAFVDAVS LSDSHEVVML LI IFA+ HSGLA+LRDQGEKLV
Sbjct: 122 LFVLNVVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLV 181

Query: 186 GERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYP 245
           GERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQL S+PG+HQLVW SSFISFFFLYP
Sbjct: 182 GERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYP 241

Query: 246 STFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIG 305
           STFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIG
Sbjct: 242 STFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIG 301

Query: 306 HHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSIT 365
           HHLFGVWNGDRRLAKRYGADFEAVKRRTSV+PFAAI+DGRQKLPDDYYKEFIRLPYLSIT
Sbjct: 302 HHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSIT 361

Query: 366 ALTMGAYLAHPLMQAASFRLHW 384
           ALT+GAYLAHPLMQAASFRLHW
Sbjct: 362 ALTIGAYLAHPLMQAASFRLHW 375

[Close]

BLAST of MC05g_new0121 vs. NCBI nr
Match: XP_022932715.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1 15-cis-zeta-carotene isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623 bits (1606), Expect = 1.03e-222
Identity = 319/381 (83.73%), Postives = 341/381 (89.50%), Query Frame = 0


Query: 7   AASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPP---YPLNCSPISGPRR 66
           A ++A  L +P   +SSNQPH RHRR DA +LR SSIS PNF P   + LN SPI   RR
Sbjct: 2   AVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRRR 61

Query: 67  LATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVL 126
                  FSA ASIGDS++GGSTS SDDDGLVGEDAAVF+LS+QKL SWAYFTVILGVVL
Sbjct: 62  -------FSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVL 121

Query: 127 FVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVG 186
           FVLNVVWIDNS  G GKAFVDA+S LSDSHEVVML LI IFA+ HSGLA+LRDQGEKLVG
Sbjct: 122 FVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVG 181

Query: 187 ERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPS 246
           ERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQL S+PG+HQLVW SSFISFFFLYPS
Sbjct: 182 ERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPS 241

Query: 247 TFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGH 306
           TFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIGH
Sbjct: 242 TFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGH 301

Query: 307 HLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITA 366
           HLFGVWNGDRRLAKRYGADFEAVKRRTSV+PFAAI+DGRQKLPDDYYKEFIRLPYLSITA
Sbjct: 302 HLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITA 361

Query: 367 LTMGAYLAHPLMQAASFRLHW 384
           LT+GAYLAHPLMQAASFRLHW
Sbjct: 362 LTIGAYLAHPLMQAASFRLHW 375

[Close]

BLAST of MC05g_new0121 vs. NCBI nr
Match: XP_023540701.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618 bits (1594), Expect = 7.48e-221
Identity = 320/383 (83.55%), Postives = 340/383 (88.77%), Query Frame = 0


Query: 7   AASAAPLLHYPLAASSSNQPHRRHR--RPDAISLRHSSISIPNFPP---YPLNCSPISGP 66
           A ++A  L +P   S SNQPH RHR  RPDA++LR SSIS PN  P   + LN SPI   
Sbjct: 2   AVASATTLFFPPFPSPSNQPHHRHRHRRPDALTLRPSSISTPNVLPQLPFSLNSSPIPRR 61

Query: 67  RRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGV 126
           RR       FSA ASIGDS++GGSTS SDDDGLVGEDAAVF+LS QKL SWAYFTVILGV
Sbjct: 62  RR-------FSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSDQKLASWAYFTVILGV 121

Query: 127 VLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKL 186
           VLFVLNVVWIDNS  G GKAFVDAVS LSDSHEVVML LI IFA+ HSGLA+LRDQGEKL
Sbjct: 122 VLFVLNVVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKL 181

Query: 187 VGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLY 246
           VGERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQL S+PG+HQLVW SSFISFFFLY
Sbjct: 182 VGERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLY 241

Query: 247 PSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLI 306
           PSTFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLI
Sbjct: 242 PSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLI 301

Query: 307 GHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSI 366
           GHHLFGVWNGDRRLAKRYGADFEAVKRRTSV+PFAAI+DGRQKLPDDYYKEFIRLPYLSI
Sbjct: 302 GHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSI 361

Query: 367 TALTMGAYLAHPLMQAASFRLHW 384
           TALT+GAYLAHPLMQAASFRLHW
Sbjct: 362 TALTIGAYLAHPLMQAASFRLHW 377

[Close]

BLAST of MC05g_new0121 vs. NCBI nr
Match: XP_038882546.1 (15-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 610 bits (1572), Expect = 1.29e-217
Identity = 316/382 (82.72%), Postives = 333/382 (87.17%), Query Frame = 0


Query: 6   AAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFP---PYPLNCSPISGPR 65
           A ASA P    P  +S      RRHRRPDA +LR  SIS PN P   P+  N +PIS   
Sbjct: 2   AVASATPPFSPPFLSS------RRHRRPDATTLRPFSISTPNCPLQLPFSFNSTPIS--- 61

Query: 66  RLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVV 125
               R   F+A ASIGDS++GGSTSVSDD G VGEDAA FDLS QKLTSW YFTVILGVV
Sbjct: 62  ----RRPRFAAEASIGDSESGGSTSVSDDGGFVGEDAAAFDLSDQKLTSWVYFTVILGVV 121

Query: 126 LFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLV 185
           LFVLNVVWIDNS  G GKAFVDAVS +SDSHEVVML+L  IFAI HSGLA+LRDQGEKLV
Sbjct: 122 LFVLNVVWIDNSAGGVGKAFVDAVSGISDSHEVVMLLLTFIFAIVHSGLASLRDQGEKLV 181

Query: 186 GERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYP 245
           GERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQLQSVPG+HQLVW SSF+SFFFLYP
Sbjct: 182 GERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYP 241

Query: 246 STFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIG 305
           STFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIG
Sbjct: 242 STFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIG 301

Query: 306 HHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSIT 365
           HHLFGVWNGDRRLAKRYGADFEAVKRRTS++PFAAIVDGRQKLPDDYYKEF+RLPYLSIT
Sbjct: 302 HHLFGVWNGDRRLAKRYGADFEAVKRRTSIVPFAAIVDGRQKLPDDYYKEFVRLPYLSIT 361

Query: 366 ALTMGAYLAHPLMQAASFRLHW 384
           ALT+GAYLAHPLMQAASFRLHW
Sbjct: 362 ALTIGAYLAHPLMQAASFRLHW 370

[Close]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL
Match: A0A6J1CUK6 (15-cis-zeta-carotene isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014723 PE=4 SV=1)

HSP 1 Score: 757 bits (1954), Expect = 6.64e-276
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG 60
           MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG
Sbjct: 1   MAVASAAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISG 60

Query: 61  PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG 120
           PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG
Sbjct: 61  PRRLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILG 120

Query: 121 VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK 180
           VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK
Sbjct: 121 VVLFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEK 180

Query: 181 LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL 240
           LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL
Sbjct: 181 LVGERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFL 240

Query: 241 YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL 300
           YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL
Sbjct: 241 YPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGL 300

Query: 301 IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS 360
           IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS
Sbjct: 301 IGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLS 360

Query: 361 ITALTMGAYLAHPLMQAASFRLHW 384
           ITALTMGAYLAHPLMQAASFRLHW
Sbjct: 361 ITALTMGAYLAHPLMQAASFRLHW 384

[Close]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL
Match: A0A6J1I3V9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470740 PE=4 SV=1)

HSP 1 Score: 623 bits (1607), Expect = 3.52e-223
Identity = 322/382 (84.29%), Postives = 340/382 (89.01%), Query Frame = 0


Query: 6   AAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPP---YPLNCSPISGPR 65
           A ASA  L   P   +SSNQPH RHRRPDA +LR SSIS PNF P   + LN SPI   R
Sbjct: 2   AVASATTLFFSPFP-TSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 61

Query: 66  RLATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVV 125
           R       FSA ASIGDS++GGSTS SDDDGLVGEDAAVF+LS+QKL SW YFTVILGVV
Sbjct: 62  R-------FSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVV 121

Query: 126 LFVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLV 185
           LFVLNVVWIDNS  G GKAFVDAVS LSDSHEVVML LI IFA+ HSGLA+LRDQGEKLV
Sbjct: 122 LFVLNVVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLV 181

Query: 186 GERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYP 245
           GERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQL S+PG+HQLVW SSFISFFFLYP
Sbjct: 182 GERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYP 241

Query: 246 STFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIG 305
           STFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIG
Sbjct: 242 STFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIG 301

Query: 306 HHLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSIT 365
           HHLFGVWNGDRRLAKRYGADFEAVKRRTSV+PFAAI+DGRQKLPDDYYKEFIRLPYLSIT
Sbjct: 302 HHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSIT 361

Query: 366 ALTMGAYLAHPLMQAASFRLHW 384
           ALT+GAYLAHPLMQAASFRLHW
Sbjct: 362 ALTIGAYLAHPLMQAASFRLHW 375

[Close]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL
Match: A0A6J1F2X1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439180 PE=4 SV=1)

HSP 1 Score: 623 bits (1606), Expect = 5.00e-223
Identity = 319/381 (83.73%), Postives = 341/381 (89.50%), Query Frame = 0


Query: 7   AASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPP---YPLNCSPISGPRR 66
           A ++A  L +P   +SSNQPH RHRR DA +LR SSIS PNF P   + LN SPI   RR
Sbjct: 2   AVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRRR 61

Query: 67  LATRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVL 126
                  FSA ASIGDS++GGSTS SDDDGLVGEDAAVF+LS+QKL SWAYFTVILGVVL
Sbjct: 62  -------FSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVL 121

Query: 127 FVLNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVG 186
           FVLNVVWIDNS  G GKAFVDA+S LSDSHEVVML LI IFA+ HSGLA+LRDQGEKLVG
Sbjct: 122 FVLNVVWIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVG 181

Query: 187 ERAFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPS 246
           ERAFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQL S+PG+HQLVW SSFISFFFLYPS
Sbjct: 182 ERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPS 241

Query: 247 TFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGH 306
           TFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIGH
Sbjct: 242 TFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGH 301

Query: 307 HLFGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITA 366
           HLFGVWNGDRRLAKRYGADFEAVKRRTSV+PFAAI+DGRQKLPDDYYKEFIRLPYLSITA
Sbjct: 302 HLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITA 361

Query: 367 LTMGAYLAHPLMQAASFRLHW 384
           LT+GAYLAHPLMQAASFRLHW
Sbjct: 362 LTIGAYLAHPLMQAASFRLHW 375

[Close]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL
Match: A0A1S3BYR9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494534 PE=4 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 1.15e-213
Identity = 305/356 (85.67%), Postives = 323/356 (90.73%), Query Frame = 0


Query: 29  RHRRPDAISLRHSSISIPNFPPYPLNCSPISGPRRLATRLGDFSAGASIGDSDTGGSTSV 88
           RHRRPDAISLR  SIS PN     L   P+S       R   F+A ASIGD ++GGSTSV
Sbjct: 19  RHRRPDAISLRPFSISAPNC----LLQLPLSLNSLHIFRRPRFAAEASIGDRESGGSTSV 78

Query: 89  SDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVWIDNSGVGFGKAFVDAVSE 148
           SDD+GLVGEDAAVFDLS+QKLTSW YFTVILGVVLFVLNVVWIDNS  G GKAF+DAVS 
Sbjct: 79  SDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWIDNSAGGVGKAFLDAVSG 138

Query: 149 LSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVLFAGISLPLAVSTVVYFIN 208
           +SDSHEVVML+L  IFAI HSGLA+LRDQGEKLVGERAFRVLFAG+SLPLAVSTVVYFIN
Sbjct: 139 ISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYFIN 198

Query: 209 HRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRI 268
           HRYDGVQLWQLQSVPG+HQLVW SSF+SFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRI
Sbjct: 199 HRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRI 258

Query: 269 TRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKR 328
           TRHPQMVGQV+WCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYG DFEAV+R
Sbjct: 259 TRHPQMVGQVIWCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGVDFEAVQR 318

Query: 329 RTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALTMGAYLAHPLMQAASFRLHW 384
           RTS+IPFAAIVDGRQKLPDDYYKEF+RLPYLSITALT+GAYLAHPLMQAASFRLHW
Sbjct: 319 RTSIIPFAAIVDGRQKLPDDYYKEFLRLPYLSITALTIGAYLAHPLMQAASFRLHW 370

[Close]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL
Match: A0A0A0KXF1 (NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 SV=1)

HSP 1 Score: 591 bits (1524), Expect = 1.37e-210
Identity = 309/379 (81.53%), Postives = 326/379 (86.02%), Query Frame = 0


Query: 6   AAASAAPLLHYPLAASSSNQPHRRHRRPDAISLRHSSISIPNFPPYPLNCSPISGPRRLA 65
           A ASA P    P  +S    P  RHRRPDAIS R  SIS PN     L   P+S      
Sbjct: 2   AVASATPPFSPPFLSS----PRHRHRRPDAISFRPFSISTPNC----LQQLPLSLNSFHI 61

Query: 66  TRLGDFSAGASIGDSDTGGSTSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFV 125
            R     A ASIGD ++GGSTSVSDD+G VGEDAA FDLS+QKLTSW YFTVILGVVLFV
Sbjct: 62  FRRPRLVAEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFV 121

Query: 126 LNVVWIDNSGVGFGKAFVDAVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGER 185
           LNVVWIDNS  G GKAF+DAVS +SDSHEVVML+LI IFAI HSGLA+LRDQGEKLVGER
Sbjct: 122 LNVVWIDNSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGER 181

Query: 186 AFRVLFAGISLPLAVSTVVYFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTF 245
           AFRVLFAG+SLPLAVSTVVYFINHRYDGVQLWQLQSVP +HQLVW SSF+SF FLYPSTF
Sbjct: 182 AFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTF 241

Query: 246 NLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHL 305
           NLLEVAAVDKPKMHLWETGIIRITRHPQMVGQV+WCLAHTIWIGNSVAVAASIGLIGHHL
Sbjct: 242 NLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVIWCLAHTIWIGNSVAVAASIGLIGHHL 301

Query: 306 FGVWNGDRRLAKRYGADFEAVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALT 365
           FGVWNGDRRLAKRYGADFEAVK RTS+IPFAAIVDGRQKLPDDYYKEF+RLPYLSITALT
Sbjct: 302 FGVWNGDRRLAKRYGADFEAVKSRTSIIPFAAIVDGRQKLPDDYYKEFLRLPYLSITALT 361

Query: 366 MGAYLAHPLMQAASFRLHW 384
           +GAYLAHPLMQAASFRLHW
Sbjct: 362 IGAYLAHPLMQAASFRLHW 372

[Close]

BLAST of MC05g_new0121 vs. TAIR 10
Match: AT1G10830.1 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 497.3 bits (1279), Expect = 1.1e-140
Identity = 236/300 (78.67%), Postives = 268/300 (89.33%), Query Frame = 0


Query: 85  STSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVWIDNSGVGFGKAFVD 144
           S S S    L+GED+A F+L KQKL SW YF V+LGVVLF+LNVVWIDNS  GFGK+F+D
Sbjct: 69  SDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNS-TGFGKSFID 128

Query: 145 AVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVLFAGISLPLAVSTVV 204
           AVS +S S EV MLMLI IFAI HSGLA+LRD GEKL+GERAFRVLFAGISLPLA+ST+V
Sbjct: 129 AVSNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIV 188

Query: 205 YFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETG 264
           YFINHRYDG QLWQLQ VPGVH+ +W ++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETG
Sbjct: 189 YFINHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETG 248

Query: 265 IIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFE 324
           I+RITRHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE
Sbjct: 249 IMRITRHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFE 308

Query: 325 AVKRRTSVIPFAAIVDGRQKLPDDYYKEFIRLPYLSITALTMGAYLAHPLMQAASFRLHW 384
           ++K+RTSVIPFAAI +GRQ LP+DYYKEF+RLPYL+ITALT+GAY AHPLMQ ASFRLHW
Sbjct: 309 SIKKRTSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

[Close]

BLAST of MC05g_new0121 vs. TAIR 10
Match: AT1G10830.2 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 302.4 bits (773), Expect = 5.3e-82
Identity = 149/198 (75.25%), Postives = 170/198 (85.86%), Query Frame = 0


Query: 85  STSVSDDDGLVGEDAAVFDLSKQKLTSWAYFTVILGVVLFVLNVVWIDNSGVGFGKAFVD 144
           S S S    L+GED+A F+L KQKL SW YF V+LGVVLF+LNVVWIDNS  GFGK+F+D
Sbjct: 69  SDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNS-TGFGKSFID 128

Query: 145 AVSELSDSHEVVMLMLISIFAIAHSGLATLRDQGEKLVGERAFRVLFAGISLPLAVSTVV 204
           AVS +S S EV MLMLI IFAI HSGLA+LRD GEKL+GERAFRVLFAGISLPLA+ST+V
Sbjct: 129 AVSNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIV 188

Query: 205 YFINHRYDGVQLWQLQSVPGVHQLVWFSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETG 264
           YFINHRYDG QLWQLQ VPGVH+ +W ++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETG
Sbjct: 189 YFINHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETG 248

Query: 265 IIRITRHPQMVGQVMWCL 283
           I+RITRHPQ++   + CL
Sbjct: 249 IMRITRHPQVLNISLMCL 265

The following BLAST results are available for this feature:

BLAST of MC05g_new0121 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAC0	1.6e-139	78.67	15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
B4FHU1	2.5e-129	62.83	15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1	[more]

BLAST of MC05g_new0121 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145219.1	1.37e-275	100.00	15-cis-zeta-carotene isomerase, chloroplastic [Momordica charantia]	[more]
XP_022972107.1	7.28e-223	84.29	15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima]	[more]
XP_022932715.1	1.03e-222	83.73	15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1...	[more]
XP_023540701.1	7.48e-221	83.55	15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_038882546.1	1.29e-217	82.72	15-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida]	[more]

BLAST of MC05g_new0121 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CUK6	6.64e-276	100.00	15-cis-zeta-carotene isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1I3V9	3.52e-223	84.29	15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1F2X1	5.00e-223	83.73	15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S3BYR9	1.15e-213	85.67	15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A0A0KXF1	1.37e-210	81.53	NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 S...	[more]

BLAST of MC05g_new0121 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10830.1	1.1e-140	78.67	15-cis-zeta-carotene isomerase	[more]
AT1G10830.2	5.3e-82	75.25	15-cis-zeta-carotene isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009915	NnrU domain	PFAM	PF07298	NnrU	coord: 156..375 e-value: 4.9E-50 score: 170.0
None	No IPR available	GENE3D	1.20.120.1630		coord: 150..335 e-value: 2.1E-12 score: 49.2
None	No IPR available	PANTHER	PTHR35988	15-CIS-ZETA-CAROTENE ISOMERASE, CHLOROPLASTIC	coord: 70..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0121.1	MC05g_new0121.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016120	carotene biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0090471	9,15,9'-tri-cis-zeta-carotene isomerase activity