MC05g_new0021 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0021
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectinesterase
Location	MC05: 2003281 .. 2006996 (-)
RNA-Seq Expression	MC05g_new0021
Synteny	MC05g_new0021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCCCCATCTTTCTTCCCTATAAAATGAGAACTCAAGCTCCACAAAAATCCCACATCTCCTCAAGAACCCAAAAAAAAAAAAGAAAAAGAAAAATGGCAACAATTAAAATTCGACAAAGCCTTTCAGTTGTTGCTCTTGTTGTTGTTCTTGTTTTTCCAAGCGCCATTTTTGGTTACTCAGAGAAAGAAATAAAATCATGGTGCAGCCAAACGCCGTATCCGGCGCCATGCGAGCACTTTCTCCGAACCAAAGCCGCCACTTCAAACAATTCTCCGATCACCGATAAATCCCATTTCCTCAAAATCCTCGTCGAAACGGCGCTCTCCACTGCCGTTTCGGCCCACAAAGACGCCATTTCCCTCGGGCCAAAGTGCCGCAACTCCCGCGAGAAATCCGCTTGGGCCGACTGCGTTTACCTCTACGACCAAATAATCTCACGGCTCAACCGGACTGCCGACCGCTGCTCCCGGTCCGACGCCCAGACCTGGCTCAGCGCCGCCCTCACCGCCCTCGAGACCTGCCGCACCGGCTTCGACGAGCTCGGGGTTTCCGCCTCCGGCTATCCCTTGACGGCGAATAACGTCTCCAAGTTGATCAGCGACGGGTTGTCGGTAAATGGACCGCCGTCGCCGGACGGGTTCGGCCCGCCGAGCCGGGTCGATGGGTTCCCGGTCTGGGTCAGCCCCGGGGACCGGAAGCTGCTGCAGTCCGGCTCGCCGGCGGGGAATGCTGACGTGGTGGTGGCGAAGGATGGGTCCGGGGACTTTCAGACGGTGGCGGCGGCGGTGGCGGCGGCGAAGGGTGGCGGGAGATTTGTGATATATGTGAAGGCGGGGGTTTATAGTGAGAATGTGGAGATCAAGGCGAAGAATGTTATGTTGGTCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGCAGAAGTGTCGGCGGCGGCTCCACCACTTTTAGATCAGCCACCGTTGGTAATTCTCTCTCTCTCTGCTTTTTATTTATTTTTTATTTCACTTTTTATTACTACTTAAAATTATATAATTATTTTCAATATAACTAAGATTTTTTAAAAAAACTAATTTAATAAATAGGTATTAATATCTATATGACAGCACAAGAATAACTTTTCTAGGTGTTAAAATGTAAATTTTAAGTGTCTTATAAGAGATTGGTATCAATGAAAAATAATAACGTTGTTGAAAGTGACATATGTAGCGACGTCCGAGAGCTTTATACATTTATTCATATCGTAACATATGATGCAAGTTAAAATTATGATAAACTTACATGTGCTGCATGGTTGTCATCAGCTCGTGTCATAACGTGTCGATTTTAGCTAATTTAGTGGAAAGAAAAACACGTACCTATTAGATTTTGTATTTACTATCAAATTTTAATTGATAAGAATATATTTTAGATAATTTAATTTTTTTTGTAAAAAAAGAGGTTAAATGTACCTAATGCCCTTGATGAGGTCACATAAGTTGACTTTATCCATTAGTCTATGGAGGAATTCAAACTGAGTTTATGGATTATTTATTTCGATTTAACGTTAATTCATGACCAATAAAAATTCAACAGTAAATACTTTGAGTAAATTGGTAAATTATTCTGACTAAATTTACCTGATAATAGAGTTGAATTATTCTTTACCAAAGCCTATAATTCTACGTTTTCAAGACTAATTAATCAAGTCATTATTATTTTTCACTTAAAATTTCGTGTTTGAATTTCATCTCTATATCTTCTCCCAATACTTTAATTTATTAATTTCAACCCTAGTTAATGATTGATGATAAATATGGGGTTCTTTTTACTCCATTAATAGATTAATTAATACAGTAAATCATTAATTATACCGGTGAAGTTAAAAAATTTGTTGAAATATAAATTAAGTCATAATGGATTTCCTAATATATGGTTTGGGATTTTATAGAAATTTAAAATGGATACAAACAAAATATTTATAGAAATATTTGTTGATGTGGCATTTTGCTTGATAGTTTATGATATGACAAAGATAAAGAAGCTTGGTTGGTTGGTGTGTAATTAATGGGACCAAAGTGTCTACTTTTTGTTACTATAAATTAAAAAAAATAATTTAATAGTGTAGCAATAGAATCTATCAATTAGTTGAGTTTAGCCTCTAAGGCAAAAAGTGACCATAATTAAATTAATTCAATAATATTCAATTGGTTTCTATGAAATTTTATGTATATGAGTGTGATGTGTGGAAACTGGCTTTTAAAGATATATTGAATAATGGCATTGTTTTTTGTTATATATATATATATATTACTTTAAAACTTTCTGTGTTATACTTTTCAAATTAGTAATTTCATGTAAAATTGAGGGACTAAAATAATATATTTACAACTTCAACAATTTAAAAACAAAAAAAAAGATTTTTTACTATATATTTTAAAATATATCACTTTTCTTTTCATACAAATATATAGCATAAATTTGACTCTTCTTAATATATTTAACTTTATCATTAAATTTTTTTTAGTACAAGATTCAATCCCACTATATTTGATTGACACTTTATCATTAAATTTGTTGAATTATGTTATTTTTGTAAATTTTAAATCCTCCGTTATATTTAAAGATATAACTGCCTTTTTATACTTTTTTTTTTTAAGTAATTGCCTATTTAAATAAAATATTTTGAATTTAGTAATGGAAAAAAAAAGAAAAAGAAAAAAGGGAAAATTGATTGCGATTTATGAGTATTATTGGTGTTAATTGGATGTCGGTTGATTTTAGCTGTCGATGGAGACGGATTCATCGCCCGTGACATTACCTTCAGAAACACCGCCGGAGCGAAGAACCACCAGGCGGTGGCGCTCCGGTCCGGCTCCGATCTCTCCGTCTTCTACCGCTGCGGATTCGAAGGCTTTCAGGACACTCTCTACGTCTACGCGGAGCGCCAATTTTACAAGCAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGAAACGCCGCCGTCGTGATCCAAGATTCCAACATAATCGCTCGCGATCCTCCTAACAAGACCATTACTCTCACCGCCCAAGGCCGGAGCGATCCGAATCAGAACACCGGAATCTCGATCCACAATTGCAGAGTCACCTCCGCCGGCGGCCTCAGCGGCGTCAAGGCCTACCTAGGGCGGCCGTGGCGCCAGTACTCGCGCACCGTTTTCATGAAATCGAACATCGGAGGATTCATAAATCCGGCCGGATGGATGGAGTGGAGCGGTAATTTTGCCCTAAATACGCTCTATTACGGCGAGTATTCGAATACCGGACCGGGATCTTCGACGGCTAATCGAGTGAAATGGAAGGGTTACCATGTGATTACCAGCGCGGCGGAGGCTTCTAAATTCACCGTCGGGAATTTCATTGCCGGCGGCTCGTGGTTGCCGAACACCGGCGTCCCCTTCACCTCCGGCCTCTGAATCTCTGTCTCTGGTTAGTGCTGTATCTATTGTTTTGTTAATCATCGCTGATTTGTTCTTCCTGAGAAAAAAACAAGAACGAATTTCATTCCAATAAACTCGTCTCTCATCAAGAAATTAGAATTTTCTTTTCCATCTCTTCTACAAAAGGAATCTAAAAAATTGTTAGGCTTCATAGGAGTATTCAATTTACAGTGTAAGTTATTTTCTTCCTTTGGAATGGTATTGTTTGAATTATTAATTTAGGTTTGTACTAAAAAAATATTATTAATT

mRNA sequence

ATGAGAACTCAAGCTCCACAAAAATCCCACATCTCCTCAAGAACCCAAAAAAAAAAAAGAAAAAGAAAAATGGCAACAATTAAAATTCGACAAAGCCTTTCAGTTGTTGCTCTTGTTGTTGTTCTTGTTTTTCCAAGCGCCATTTTTGGTTACTCAGAGAAAGAAATAAAATCATGGTGCAGCCAAACGCCGTATCCGGCGCCATGCGAGCACTTTCTCCGAACCAAAGCCGCCACTTCAAACAATTCTCCGATCACCGATAAATCCCATTTCCTCAAAATCCTCGTCGAAACGGCGCTCTCCACTGCCGTTTCGGCCCACAAAGACGCCATTTCCCTCGGGCCAAAGTGCCGCAACTCCCGCGAGAAATCCGCTTGGGCCGACTGCGTTTACCTCTACGACCAAATAATCTCACGGCTCAACCGGACTGCCGACCGCTGCTCCCGGTCCGACGCCCAGACCTGGCTCAGCGCCGCCCTCACCGCCCTCGAGACCTGCCGCACCGGCTTCGACGAGCTCGGGGTTTCCGCCTCCGGCTATCCCTTGACGGCGAATAACGTCTCCAAGTTGATCAGCGACGGGTTGTCGGTAAATGGACCGCCGTCGCCGGACGGGTTCGGCCCGCCGAGCCGGGTCGATGGGTTCCCGGTCTGGGTCAGCCCCGGGGACCGGAAGCTGCTGCAGTCCGGCTCGCCGGCGGGGAATGCTGACGTGGTGGTGGCGAAGGATGGGTCCGGGGACTTTCAGACGGTGGCGGCGGCGGTGGCGGCGGCGAAGGGTGGCGGGAGATTTGTGATATATGTGAAGGCGGGGGTTTATAGTGAGAATGTGGAGATCAAGGCGAAGAATGTTATGTTGGTCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGCAGAAGTGTCGGCGGCGGCTCCACCACTTTTAGATCAGCCACCGTTGCTGTCGATGGAGACGGATTCATCGCCCGTGACATTACCTTCAGAAACACCGCCGGAGCGAAGAACCACCAGGCGGTGGCGCTCCGGTCCGGCTCCGATCTCTCCGTCTTCTACCGCTGCGGATTCGAAGGCTTTCAGGACACTCTCTACGTCTACGCGGAGCGCCAATTTTACAAGCAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGAAACGCCGCCGTCGTGATCCAAGATTCCAACATAATCGCTCGCGATCCTCCTAACAAGACCATTACTCTCACCGCCCAAGGCCGGAGCGATCCGAATCAGAACACCGGAATCTCGATCCACAATTGCAGAGTCACCTCCGCCGGCGGCCTCAGCGGCGTCAAGGCCTACCTAGGGCGGCCGTGGCGCCAGTACTCGCGCACCGTTTTCATGAAATCGAACATCGGAGGATTCATAAATCCGGCCGGATGGATGGAGTGGAGCGGTAATTTTGCCCTAAATACGCTCTATTACGGCGAGTATTCGAATACCGGACCGGGATCTTCGACGGCTAATCGAGTGAAATGGAAGGGTTACCATGTGATTACCAGCGCGGCGGAGGCTTCTAAATTCACCGTCGGGAATTTCATTGCCGGCGGCTCGTGGTTGCCGAACACCGGCGTCCCCTTCACCTCCGGCCTCTGA

Coding sequence (CDS)

ATGAGAACTCAAGCTCCACAAAAATCCCACATCTCCTCAAGAACCCAAAAAAAAAAAAGAAAAAGAAAAATGGCAACAATTAAAATTCGACAAAGCCTTTCAGTTGTTGCTCTTGTTGTTGTTCTTGTTTTTCCAAGCGCCATTTTTGGTTACTCAGAGAAAGAAATAAAATCATGGTGCAGCCAAACGCCGTATCCGGCGCCATGCGAGCACTTTCTCCGAACCAAAGCCGCCACTTCAAACAATTCTCCGATCACCGATAAATCCCATTTCCTCAAAATCCTCGTCGAAACGGCGCTCTCCACTGCCGTTTCGGCCCACAAAGACGCCATTTCCCTCGGGCCAAAGTGCCGCAACTCCCGCGAGAAATCCGCTTGGGCCGACTGCGTTTACCTCTACGACCAAATAATCTCACGGCTCAACCGGACTGCCGACCGCTGCTCCCGGTCCGACGCCCAGACCTGGCTCAGCGCCGCCCTCACCGCCCTCGAGACCTGCCGCACCGGCTTCGACGAGCTCGGGGTTTCCGCCTCCGGCTATCCCTTGACGGCGAATAACGTCTCCAAGTTGATCAGCGACGGGTTGTCGGTAAATGGACCGCCGTCGCCGGACGGGTTCGGCCCGCCGAGCCGGGTCGATGGGTTCCCGGTCTGGGTCAGCCCCGGGGACCGGAAGCTGCTGCAGTCCGGCTCGCCGGCGGGGAATGCTGACGTGGTGGTGGCGAAGGATGGGTCCGGGGACTTTCAGACGGTGGCGGCGGCGGTGGCGGCGGCGAAGGGTGGCGGGAGATTTGTGATATATGTGAAGGCGGGGGTTTATAGTGAGAATGTGGAGATCAAGGCGAAGAATGTTATGTTGGTCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGCAGAAGTGTCGGCGGCGGCTCCACCACTTTTAGATCAGCCACCGTTGCTGTCGATGGAGACGGATTCATCGCCCGTGACATTACCTTCAGAAACACCGCCGGAGCGAAGAACCACCAGGCGGTGGCGCTCCGGTCCGGCTCCGATCTCTCCGTCTTCTACCGCTGCGGATTCGAAGGCTTTCAGGACACTCTCTACGTCTACGCGGAGCGCCAATTTTACAAGCAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGAAACGCCGCCGTCGTGATCCAAGATTCCAACATAATCGCTCGCGATCCTCCTAACAAGACCATTACTCTCACCGCCCAAGGCCGGAGCGATCCGAATCAGAACACCGGAATCTCGATCCACAATTGCAGAGTCACCTCCGCCGGCGGCCTCAGCGGCGTCAAGGCCTACCTAGGGCGGCCGTGGCGCCAGTACTCGCGCACCGTTTTCATGAAATCGAACATCGGAGGATTCATAAATCCGGCCGGATGGATGGAGTGGAGCGGTAATTTTGCCCTAAATACGCTCTATTACGGCGAGTATTCGAATACCGGACCGGGATCTTCGACGGCTAATCGAGTGAAATGGAAGGGTTACCATGTGATTACCAGCGCGGCGGAGGCTTCTAAATTCACCGTCGGGAATTTCATTGCCGGCGGCTCGTGGTTGCCGAACACCGGCGTCCCCTTCACCTCCGGCCTCTGA

Protein sequence

MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL

Homology

[Close]

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 7.3e-183
Identity = 323/514 (62.84%), Postives = 393/514 (76.46%), Query Frame = 0


Query: 35  VVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKI 94
           +   +V+       FGYS +E+KSWC +TP P PCE+FL  K   ++    TD   F KI
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTD---FYKI 66

Query: 95  LVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADR---CSRSD 154
            ++ AL  A +A     +LG KCRN REK+AW DC  LY+  + +LN+T++    C++ D
Sbjct: 67  SLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 126

Query: 155 AQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSR 214
            QTWLS ALT LETCR   ++LGV     PL +NNV+KLIS+ LS+N  P    +  PS 
Sbjct: 127 KQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPSY 186

Query: 215 VDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAK--GGGRFVIYVK 274
            DGFP WV PGDRKLLQ+      A++VVA+DGSG+ +T+  AVAAA   GG R+VIY+K
Sbjct: 187 KDGFPTWVKPGDRKLLQT---TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIK 246

Query: 275 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 334
           AG Y+EN+E+K KN+M VGDGIGKT+ITGS+SVGGG+TTF+SATVAV GD FIARDIT R
Sbjct: 247 AGTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIR 306

Query: 335 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 394
           NTAG  NHQAVALRSGSDLSVFYRC FEG+QDTLYV+++RQFY++CDIYGTVDFIFGNAA
Sbjct: 307 NTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAA 366

Query: 395 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGL----SGVKAYLGR 454
           VV+Q+ NI AR PPN+T TLTAQGR+DPNQ+TGI IHNCRVT+A  L    S VK +LGR
Sbjct: 367 VVLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGR 426

Query: 455 PWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYH 514
           PW+QYSRTV++K+ +   INPAGWMEWSG+FALNTLYY EY NTGPGSSTANRVKW+GYH
Sbjct: 427 PWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYH 486

Query: 515 VITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           V+TS ++ S+FTVGNFIAG SWLP T VPFTSGL
Sbjct: 487 VLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 639.0 bits (1647), Expect = 4.7e-182
Identity = 322/509 (63.26%), Postives = 394/509 (77.41%), Query Frame = 0


Query: 38  LVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVE 97
           +++ ++  S + GY++K++K+WCSQTP P PCE+FL      SNN PI  +S FLKI ++
Sbjct: 12  VILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL---THNSNNEPIKSESEFLKISMK 71

Query: 98  TALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD---RCSRSDAQT 157
             L  A+ A   A +LGPKCR++REK+AW DC+ LYD  +S++N T D   +CS+ DAQT
Sbjct: 72  LVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQT 131

Query: 158 WLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDG 217
           WLS ALT L+TCR GF ELGV+    PL +NNVS L+ + L++N    P  + PP + DG
Sbjct: 132 WLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--KVPFNYTPPEK-DG 191

Query: 218 FPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYS 277
           FP WV PGDRKLLQS +P  NA  VVAKDGSG+F+T+  A+ AA G GRFVIYVK GVYS
Sbjct: 192 FPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYS 251

Query: 278 ENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 337
           EN+EI+ KNVML GDGIGKT+ITGS+SVGGG+TTF SATVA  GDGFIAR ITFRNTAGA
Sbjct: 252 ENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 311

Query: 338 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 397
            N QAVALRSGSDLSVFY+C FE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V+Q+
Sbjct: 312 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 371

Query: 398 SNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQY 457
            NI AR P +KT T+TAQGRSDPNQNTGI IHN RVT+A  L  V    K YLGRPWRQY
Sbjct: 372 CNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQY 431

Query: 458 SRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSA 517
           SRTVFMK+++   I+P GW+EW GNFAL TL+Y E+ NTGPG+ST+ RV W G+ V+ SA
Sbjct: 432 SRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSA 491

Query: 518 AEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           +EASKFTVG F+AGGSW+P++ VPFTSGL
Sbjct: 492 SEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 253/508 (49.80%), Postives = 331/508 (65.16%), Query Frame = 0


Query: 59  WCSQTPYPAPCEHFLRTKAATS-NNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKC 118
           WC++TP+   C ++ R  +  + N  P   +S FL++LV+ AL  AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 119 RNSREKSAWADCVYLYDQIISRLNRTAD----------RCSRSDAQTWLSAALTALETCR 178
            N++ K+AW+DCV L+   +++LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 179 TGFDELGVSASGYPLTAN-NVSKLISDGLSVNG--PPSPDGFGPPSRVDGFPVWVSPGDR 238
           +G ++L VS    P+ +N N+S LI + L+VNG      D     +  + FP WVS  +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 239 KLLQSGSPA-GNADVVVAKDGSGDFQTVAAAV---AAAKGGGRFVIYVKAGVYSENVEI- 298
           +LL S S A  +  +VVA+D SG F+++ AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 299 -KAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 358
               N+MLVGDG  KT+IT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 359 AVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNII 418
           AVA+RS SDLSVFYR G  GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+  I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 419 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQYSRT 478
            R P   +   +TAQGR DP QNTGI+IH+ R+ +A  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 479 VFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAA 538
             MK+ I   I+P GW  W    NFALNT++YGEY N GPGSST  RV+WKG+H ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

Query: 539 EASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            AS+FTVG+ IAGGSWLP TGVPF SGL
Sbjct: 567 VASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 256/533 (48.03%), Postives = 339/533 (63.60%), Query Frame = 0


Query: 33  LSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFL 92
           L ++ LV++ V P  +   +++    WC +TPYP PC+ + +     +     T  S F 
Sbjct: 13  LHLILLVLLCVHP--LTTVADRNSTDWCDKTPYPDPCKCYFKNH---NGFQQPTQLSEFR 72

Query: 93  KILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRT--------- 152
            +LVE A+  A+SA  +  + G  C +S++++  ADC+ LY   I +LNRT         
Sbjct: 73  VMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAG 132

Query: 153 -ADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 212
            A  C+  DAQTWLS ALT  ETCR G  +L V+    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 AAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGAL 192

Query: 213 SPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 272
              G      +   GFP W+S  D++LL+    A  A++VVAKDGSG F TV AA+  A 
Sbjct: 193 LTAGNKGNTTANQKGFPTWLSRKDKRLLR----AVRANLVVAKDGSGHFNTVQAAIDVAG 252

Query: 273 ---KGGGRFVIYVKAGVYSENVEIKAKN--VMLVGDGIGKTVITGSRSVGGGSTTFRSAT 332
                 GRFVIYVK G+Y EN+ ++  N  +MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 312

Query: 333 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 392
             ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 372

Query: 393 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 452
           +C IYGTVDFIFGNAA V Q+  I+ R P   +   +TAQGR+DP QNTGISIHN R+  
Sbjct: 373 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 432

Query: 453 AGGL----SGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEY 512
           A  L      VK Y+GRPW ++SRTV +++ +   ++P GW  W     F L+TL+Y EY
Sbjct: 433 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 492

Query: 513 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            NTGP SST  RV WKG+HV+  A++AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 493 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot
Match: O81320 (Putative pectinesterase/pectinesterase inhibitor 38 OS=Arabidopsis thaliana OX=3702 GN=PME38 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 6.5e-123
Identity = 247/494 (50.00%), Postives = 314/494 (63.56%), Query Frame = 0


Query: 60  CSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRN 119
           C +TP+P  C      K     + PI   + FL++ VE  L  AV A  D   L P+  +
Sbjct: 8   CDETPHPGEC------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS 67

Query: 120 SREKSAWADCVYLYDQIISRLNRTA----DRCSRSDAQTWLSAALTALETCRTGFDELGV 179
              K AW +C+ LY+Q I RLN +     + CSRSD Q WLS ALT L+TC+    ELGV
Sbjct: 68  ---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGV 127

Query: 180 SASGYPLTANNVSKLISDGLSVNGPPSPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPA 239
           S+     +  +++  + + L++N     +G  FG           +S    K L  G   
Sbjct: 128 SSH----SLESITIDVINTLAINKRMEQNGKEFG-----------ISKITMKTLSIGE-- 187

Query: 240 GNADVVVAKDGSGDFQTVAAAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLV 299
              DVVVA+DGSGD++T+  AV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ 
Sbjct: 188 -KVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMIT 247

Query: 300 GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSD 359
           GDGIGKT+ITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD
Sbjct: 248 GDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSD 307

Query: 360 LSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTI 419
           +SVFYRC  EG+QDTLYV++ RQF+++CDIYGTVDFIFGNAA V+Q+  I AR+PPN   
Sbjct: 308 MSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVN 367

Query: 420 TLTAQGRSDPNQNTGISIHNCRVTSAGG--LSGVKAYLGRPWRQYSRTVFMKSNIGGFIN 479
           T+TAQ R +PNQ TGI IHN  V  A G  L GVK YLGRPWR Y+RTV + + +   I 
Sbjct: 368 TITAQSRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIE 427

Query: 480 PAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGG 539
           P GW++W    AL+TLYYGEY N+GPGS T NRV W G+HVI+   EA +FT+  FI   
Sbjct: 428 PNGWIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSA 474

[Close]

BLAST of MC05g_new0021 vs. NCBI nr
Match: XP_022147136.1 (pectinesterase 2 [Momordica charantia])

HSP 1 Score: 1067 bits (2759), Expect = 0.0
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60
           MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC
Sbjct: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60

Query: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120
           SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS
Sbjct: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120

Query: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180
           REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY
Sbjct: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180

Query: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240
           PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV
Sbjct: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240

Query: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300
           AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR
Sbjct: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300

Query: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360
           SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ
Sbjct: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360

Query: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420
           DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN
Sbjct: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420

Query: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480
           TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT
Sbjct: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480

Query: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL
Sbjct: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

[Close]

BLAST of MC05g_new0021 vs. NCBI nr
Match: XP_038877776.1 (pectinesterase 2 [Benincasa hispida])

HSP 1 Score: 870 bits (2247), Expect = 1.51e-315
Identity = 434/504 (86.11%), Postives = 464/504 (92.06%), Query Frame = 0


Query: 36  VALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKIL 95
           V +V+VL+FP  I+ YSE EIK+WCSQTPYPAPCE FL+TKA T+  SPI  KSHF +IL
Sbjct: 9   VVVVIVLIFPLDIYCYSEIEIKNWCSQTPYPAPCEEFLKTKA-TTKRSPIATKSHFFEIL 68

Query: 96  VETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSRSDAQTW 155
           VETAL  AVSA ++A+SLGPKCRNS+E++AW DCV LYDQII+RLNRT DRC+  DAQTW
Sbjct: 69  VETALERAVSARQNALSLGPKCRNSKERTAWTDCVDLYDQIITRLNRTVDRCTPVDAQTW 128

Query: 156 LSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGF 215
           LSAALTALETCRTG +ELGVSA GYPLTANNVSKLISDGLSVN P SP+GF PP+  DGF
Sbjct: 129 LSAALTALETCRTGLEELGVSAFGYPLTANNVSKLISDGLSVNKPASPEGFEPPTMEDGF 188

Query: 216 PVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSE 275
           P WV PGDR+LLQSGSP  N  VVVA+DGSG+F+TV  AVAAAKGGGRFVIYVKAGVYSE
Sbjct: 189 PTWVRPGDRRLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248

Query: 276 NVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAK 335
           NVEI AKNVML+GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAK
Sbjct: 249 NVEINAKNVMLIGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAK 308

Query: 336 NHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDS 395
           NHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 
Sbjct: 309 NHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDC 368

Query: 396 NIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVF 455
           NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWRQYSRTV 
Sbjct: 369 NIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWRQYSRTVV 428

Query: 456 MKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASK 515
           MKS+IGGFI+PAGWMEWSGNFALNTLYYGEY NTGPG+STANRVKWKGY VITSAAEASK
Sbjct: 429 MKSSIGGFISPAGWMEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSAAEASK 488

Query: 516 FTVGNFIAGGSWLPNTGVPFTSGL 539
           FTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 489 FTVGNFIAGGSWLPSTGVPFTSGL 509

[Close]

BLAST of MC05g_new0021 vs. NCBI nr
Match: XP_008438193.1 (PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 869 bits (2246), Expect = 3.01e-315
Identity = 429/511 (83.95%), Postives = 472/511 (92.37%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVD-GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + +D GFP WV PGDRKLLQSGSP  N  VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. NCBI nr
Match: TYK17508.1 (pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 867 bits (2239), Expect = 3.01e-314
Identity = 428/511 (83.76%), Postives = 471/511 (92.17%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVD-GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + +D GFP WV PGDRKLLQSGSP  N  VVVA+DGSG+F+TV  AVAAAK GGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. NCBI nr
Match: XP_004133929.1 (pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [Cucumis sativus])

HSP 1 Score: 864 bits (2232), Expect = 3.26e-313
Identity = 425/510 (83.33%), Postives = 470/510 (92.16%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R ++    +++VL+FPS I  YSEKEIK+WCSQTPYPAPCE FL+TKA T+  +PIT KS
Sbjct: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKA-TTKKTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYDQII+RLNRT+ RCS 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGF+ELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVK 269
           +  DGFP WVSPG+RKLLQS SP   ADVVVA+DGSG+F+TV  A++AAKGGGRFVIY+K
Sbjct: 183 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242

Query: 270 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 329
           +GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR
Sbjct: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302

Query: 330 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 389
           NTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNAA
Sbjct: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362

Query: 390 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQ 449
           VV+QD NIIARDPPNKTITLTAQGRSDPNQNTGISIHNCR+TS+GGLSGVKAYLGRPWRQ
Sbjct: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQ 422

Query: 450 YSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITS 509
           YSRTV MKS+IGGFI+PAGWM WSGNFALNTLYY EY NTGPG+STANRV WKGYHVITS
Sbjct: 423 YSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITS 482

Query: 510 AAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           A+EASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 ASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

[Close]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL
Match: A0A6J1D1F9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016139 PE=3 SV=1)

HSP 1 Score: 1067 bits (2759), Expect = 0.0
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60
           MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC
Sbjct: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60

Query: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120
           SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS
Sbjct: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120

Query: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180
           REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY
Sbjct: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180

Query: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240
           PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV
Sbjct: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240

Query: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300
           AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR
Sbjct: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300

Query: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360
           SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ
Sbjct: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360

Query: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420
           DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN
Sbjct: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420

Query: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480
           TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT
Sbjct: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480

Query: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL
Sbjct: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

[Close]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 869 bits (2246), Expect = 1.46e-315
Identity = 429/511 (83.95%), Postives = 472/511 (92.37%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVD-GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + +D GFP WV PGDRKLLQSGSP  N  VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL
Match: A0A1S3AVW4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 869 bits (2246), Expect = 1.46e-315
Identity = 429/511 (83.95%), Postives = 472/511 (92.37%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVD-GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + +D GFP WV PGDRKLLQSGSP  N  VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL
Match: A0A5D3D1Y4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003750 PE=3 SV=1)

HSP 1 Score: 867 bits (2239), Expect = 1.46e-314
Identity = 428/511 (83.76%), Postives = 471/511 (92.17%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVD-GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + +D GFP WV PGDRKLLQSGSP  N  VVVA+DGSG+F+TV  AVAAAK GGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSPKAN--VVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 864 bits (2232), Expect = 1.58e-313
Identity = 425/510 (83.33%), Postives = 470/510 (92.16%), Query Frame = 0


Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R ++    +++VL+FPS I  YSEKEIK+WCSQTPYPAPCE FL+TKA T+  +PIT KS
Sbjct: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKA-TTKKTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYDQII+RLNRT+ RCS 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGF+ELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVK 269
           +  DGFP WVSPG+RKLLQS SP   ADVVVA+DGSG+F+TV  A++AAKGGGRFVIY+K
Sbjct: 183 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242

Query: 270 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 329
           +GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR
Sbjct: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302

Query: 330 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 389
           NTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNAA
Sbjct: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362

Query: 390 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQ 449
           VV+QD NIIARDPPNKTITLTAQGRSDPNQNTGISIHNCR+TS+GGLSGVKAYLGRPWRQ
Sbjct: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQ 422

Query: 450 YSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITS 509
           YSRTV MKS+IGGFI+PAGWM WSGNFALNTLYY EY NTGPG+STANRV WKGYHVITS
Sbjct: 423 YSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITS 482

Query: 510 AAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539
           A+EASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 ASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

[Close]

BLAST of MC05g_new0021 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 639.0 bits (1647), Expect = 3.3e-183
Identity = 322/509 (63.26%), Postives = 394/509 (77.41%), Query Frame = 0


Query: 38  LVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVE 97
           +++ ++  S + GY++K++K+WCSQTP P PCE+FL      SNN PI  +S FLKI ++
Sbjct: 12  VILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL---THNSNNEPIKSESEFLKISMK 71

Query: 98  TALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD---RCSRSDAQT 157
             L  A+ A   A +LGPKCR++REK+AW DC+ LYD  +S++N T D   +CS+ DAQT
Sbjct: 72  LVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQT 131

Query: 158 WLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDG 217
           WLS ALT L+TCR GF ELGV+    PL +NNVS L+ + L++N    P  + PP + DG
Sbjct: 132 WLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--KVPFNYTPPEK-DG 191

Query: 218 FPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYS 277
           FP WV PGDRKLLQS +P  NA  VVAKDGSG+F+T+  A+ AA G GRFVIYVK GVYS
Sbjct: 192 FPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYS 251

Query: 278 ENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 337
           EN+EI+ KNVML GDGIGKT+ITGS+SVGGG+TTF SATVA  GDGFIAR ITFRNTAGA
Sbjct: 252 ENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 311

Query: 338 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 397
            N QAVALRSGSDLSVFY+C FE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V+Q+
Sbjct: 312 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 371

Query: 398 SNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQY 457
            NI AR P +KT T+TAQGRSDPNQNTGI IHN RVT+A  L  V    K YLGRPWRQY
Sbjct: 372 CNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQY 431

Query: 458 SRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSA 517
           SRTVFMK+++   I+P GW+EW GNFAL TL+Y E+ NTGPG+ST+ RV W G+ V+ SA
Sbjct: 432 SRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSA 491

Query: 518 AEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           +EASKFTVG F+AGGSW+P++ VPFTSGL
Sbjct: 492 SEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of MC05g_new0021 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 253/508 (49.80%), Postives = 331/508 (65.16%), Query Frame = 0


Query: 59  WCSQTPYPAPCEHFLRTKAATS-NNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKC 118
           WC++TP+   C ++ R  +  + N  P   +S FL++LV+ AL  AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 119 RNSREKSAWADCVYLYDQIISRLNRTAD----------RCSRSDAQTWLSAALTALETCR 178
            N++ K+AW+DCV L+   +++LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 179 TGFDELGVSASGYPLTAN-NVSKLISDGLSVNG--PPSPDGFGPPSRVDGFPVWVSPGDR 238
           +G ++L VS    P+ +N N+S LI + L+VNG      D     +  + FP WVS  +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 239 KLLQSGSPA-GNADVVVAKDGSGDFQTVAAAV---AAAKGGGRFVIYVKAGVYSENVEI- 298
           +LL S S A  +  +VVA+D SG F+++ AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 299 -KAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 358
               N+MLVGDG  KT+IT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 359 AVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNII 418
           AVA+RS SDLSVFYR G  GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+  I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 419 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQYSRT 478
            R P   +   +TAQGR DP QNTGI+IH+ R+ +A  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 479 VFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAA 538
             MK+ I   I+P GW  W    NFALNT++YGEY N GPGSST  RV+WKG+H ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

Query: 539 EASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            AS+FTVG+ IAGGSWLP TGVPF SGL
Sbjct: 567 VASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of MC05g_new0021 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 256/533 (48.03%), Postives = 339/533 (63.60%), Query Frame = 0


Query: 33  LSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFL 92
           L ++ LV++ V P  +   +++    WC +TPYP PC+ + +     +     T  S F 
Sbjct: 13  LHLILLVLLCVHP--LTTVADRNSTDWCDKTPYPDPCKCYFKNH---NGFQQPTQLSEFR 72

Query: 93  KILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRT--------- 152
            +LVE A+  A+SA  +  + G  C +S++++  ADC+ LY   I +LNRT         
Sbjct: 73  VMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAG 132

Query: 153 -ADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 212
            A  C+  DAQTWLS ALT  ETCR G  +L V+    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 AAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGAL 192

Query: 213 SPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 272
              G      +   GFP W+S  D++LL+    A  A++VVAKDGSG F TV AA+  A 
Sbjct: 193 LTAGNKGNTTANQKGFPTWLSRKDKRLLR----AVRANLVVAKDGSGHFNTVQAAIDVAG 252

Query: 273 ---KGGGRFVIYVKAGVYSENVEIKAKN--VMLVGDGIGKTVITGSRSVGGGSTTFRSAT 332
                 GRFVIYVK G+Y EN+ ++  N  +MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 312

Query: 333 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 392
             ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 372

Query: 393 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 452
           +C IYGTVDFIFGNAA V Q+  I+ R P   +   +TAQGR+DP QNTGISIHN R+  
Sbjct: 373 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 432

Query: 453 AGGL----SGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEY 512
           A  L      VK Y+GRPW ++SRTV +++ +   ++P GW  W     F L+TL+Y EY
Sbjct: 433 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 492

Query: 513 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            NTGP SST  RV WKG+HV+  A++AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 493 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of MC05g_new0021 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 442.6 bits (1137), Expect = 4.6e-124
Identity = 247/494 (50.00%), Postives = 314/494 (63.56%), Query Frame = 0


Query: 60  CSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRN 119
           C +TP+P  C      K     + PI   + FL++ VE  L  AV A  D   L P+  +
Sbjct: 8   CDETPHPGEC------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS 67

Query: 120 SREKSAWADCVYLYDQIISRLNRTA----DRCSRSDAQTWLSAALTALETCRTGFDELGV 179
              K AW +C+ LY+Q I RLN +     + CSRSD Q WLS ALT L+TC+    ELGV
Sbjct: 68  ---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGV 127

Query: 180 SASGYPLTANNVSKLISDGLSVNGPPSPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPA 239
           S+     +  +++  + + L++N     +G  FG           +S    K L  G   
Sbjct: 128 SSH----SLESITIDVINTLAINKRMEQNGKEFG-----------ISKITMKTLSIGE-- 187

Query: 240 GNADVVVAKDGSGDFQTVAAAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLV 299
              DVVVA+DGSGD++T+  AV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ 
Sbjct: 188 -KVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMIT 247

Query: 300 GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSD 359
           GDGIGKT+ITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD
Sbjct: 248 GDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSD 307

Query: 360 LSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTI 419
           +SVFYRC  EG+QDTLYV++ RQF+++CDIYGTVDFIFGNAA V+Q+  I AR+PPN   
Sbjct: 308 MSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVN 367

Query: 420 TLTAQGRSDPNQNTGISIHNCRVTSAGG--LSGVKAYLGRPWRQYSRTVFMKSNIGGFIN 479
           T+TAQ R +PNQ TGI IHN  V  A G  L GVK YLGRPWR Y+RTV + + +   I 
Sbjct: 368 TITAQSRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIE 427

Query: 480 PAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGG 539
           P GW++W    AL+TLYYGEY N+GPGS T NRV W G+HVI+   EA +FT+  FI   
Sbjct: 428 PNGWIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSA 474

[Close]

BLAST of MC05g_new0021 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 441.8 bits (1135), Expect = 7.8e-124
Identity = 254/533 (47.65%), Postives = 336/533 (63.04%), Query Frame = 0


Query: 32  SLSVVALVVVLVFP-SAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSH 91
           SL ++ ++++ + P + +   +   I  WC +TPYP PC+ +       S     T  S 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF---IKHSGFRLPTQISE 72

Query: 92  FLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD----- 151
           F  +LVE A+  AVSA     +    C + ++++  ADC+ LY   + +LNRT       
Sbjct: 73  FRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSK 132

Query: 152 ---RCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 211
              RC+  DAQTWLS ALT  ETCR G  +L VS    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 TGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGAL 192

Query: 212 SPDGFGPPSRVD--GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 271
              G    +  D  GFP WVS  +R+LLQ  S    A++VVAKDGSG F+TV AA+  A 
Sbjct: 193 LTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSV--RANLVVAKDGSGHFKTVQAAIDVAG 252

Query: 272 ---KGGGRFVIYVKAGVYSENVEIKAK--NVMLVGDGIGKTVITGSRSVGGGSTTFRSAT 331
                 GRFVIYVK G+Y EN+ ++    N+MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 312

Query: 332 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 391
             ++G  FIA+ I F+NTAG    QAVALRS SDLS+FYRC  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYR 372

Query: 392 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 451
           +C IYGTVDFIFGNAAVV Q+  I+ R P   +   +TAQGR+D  QNTGISIHN  +  
Sbjct: 373 ECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIP 432

Query: 452 AGGLS----GVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWS--GNFALNTLYYGEY 511
           A  L      VK Y+GRPW  YSRTV +K+ I   ++P GW  W+    + L+TL+Y EY
Sbjct: 433 APDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEY 492

Query: 512 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            N GP SST  RV+WKG+HV++ A++AS F+VG FIAG +WLP +G+PFTS L
Sbjct: 493 KNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

The following BLAST results are available for this feature:

BLAST of MC05g_new0021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	7.3e-183	62.84	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	4.7e-182	63.26	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	1.6e-126	49.80	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	1.6e-126	48.03	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
O81320	6.5e-123	50.00	Putative pectinesterase/pectinesterase inhibitor 38 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MC05g_new0021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147136.1	0.0	100.00	pectinesterase 2 [Momordica charantia]	[more]
XP_038877776.1	1.51e-315	86.11	pectinesterase 2 [Benincasa hispida]	[more]
XP_008438193.1	3.01e-315	83.95	PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucum...	[more]
TYK17508.1	3.01e-314	83.76	pectinesterase 2 [Cucumis melo var. makuwa]	[more]
XP_004133929.1	3.26e-313	83.33	pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [...	[more]

BLAST of MC05g_new0021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1F9	0.0	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016139 PE=3 SV=1	[more]
A0A5A7U603	1.46e-315	83.95	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00283...	[more]
A0A1S3AVW4	1.46e-315	83.95	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1	[more]
A0A5D3D1Y4	1.46e-314	83.76	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00375...	[more]
A0A0A0L7N4	1.58e-313	83.33	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1	[more]

BLAST of MC05g_new0021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	3.3e-183	63.26	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	1.2e-127	49.80	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	1.2e-127	48.03	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	4.6e-124	50.00	pectin methylesterase 38	[more]
AT5G51500.1	7.8e-124	47.65	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 51..197 e-value: 5.4E-32 score: 122.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 55..193 e-value: 2.4E-24 score: 86.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 31..189 e-value: 1.1E-25 score: 88.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 51..199 e-value: 4.3E-27 score: 97.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 54..197
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 237..524 e-value: 3.3E-134 score: 446.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 233..539 e-value: 2.8E-120 score: 403.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 33..538
None	No IPR available	PANTHER	PTHR31707:SF160	PECTINESTERASE	coord: 33..538
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 58..202 e-value: 9.58974E-30 score: 112.532
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 377..386
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 234..538

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g_new0021.1	MC05g_new0021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity