MC05g_new0004 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g_new0004
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionFHA domain-containing protein At4g14490-like
LocationMC05: 431805 .. 433916 (+)
RNA-Seq ExpressionMC05g_new0004
SyntenyMC05g_new0004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGGTCGAGATGCAAATACAGACCAGCCCATTTAGTTTATTGCACTAAAAAAGTACAGCCCATTTAGGTGGCCCAAAAGCTGAGGCAAGAGAAGTAGAATTGGAGGGAAGCAAAACTTCGAAGTTCAAACTCGAGCCGCGCGTTCCATTTTCCCCATTTTAGAATTATCAATTAGGTGAAGACTGAATAGTGAAGAGTCTGAAGACCTTCTCAATTCAATTCCAATTTTCAATTTTCAGCAGCGAGAATTCAAATTCAGAGGCTTCACTTCACATCCTCTCAATGGAATCCTCTGCAATTACTCTCATTATAGTTCAAGGCCCTCGCGAGGGCGAAACCCTAGAATTTCCATCCGGAACCACGATTCGGATTGGTCGCGTTGTTCGGGGTAACTCTATCGCCATTAAAGATGCTGGCATCTCCACCAAGCACCTTTCTATCCAACCTGAGCCTGTATCAGGTAAGTGGGTACTCCAGGACCTCGATTCGTCCAATGGCACATTTGTTAACGATACCAAGCTTCCTCCACACGATGCATTCGCTCTGAGCGACGGCGACACCATTAAATTCGGCGAGTGGACCTCGATTTTGGTCAGAATAAACACTAATGAAGAGTGCCGTTCGAGGCGAAACCCAAGGCGGAAAGCTGCAGAGAAGTGTACAGGTGCGGATGTGGTGGGATCGGTTGCGCAAACTCGGGGTCGAAGAGGAAAGGCTGTGGAAGAGGTCAGCGTACTTGGGGGAGGTAATGACGCGGGGTTGGAGAGTGGAAGATGTCTAAGGCCGAGGAGAGGCAGGGGTGTGAAAGCTGAAATTGATAACGAAGTACCGGATTGCAAAAAAATTGAGGACAATTTAGATGTGGGAAGAGGGTTAGAGAGTGTGACTGATCTGGAAAACGAACCCAGCCCAAAAATTACGGCAAGAAGTACCCGGAGAGCGAAGAATGATGTGCGTGTGGCTGCAGATTCAGTTATCGGGAAGGTTCCCGAGAATTCATGTGTGGGCGGTGAAGTAAAGGCAGGGGCAAAAAAGACGAGGGGAACTAGGGGAAGGAAGAAACTGCAAAATGAGCCGCCGGATTGCAGTACAGTTACAAAATTGGAACACACGGAAGCCATAGAGCAGAGGAGCTCAGGAGAAAATACCTTGGTTGACGATGAAGAAGGCAAGAAGAATGTAAACGTTGAAGAGACTGGTAGCGGGTCTTACCCTCAAGAGGCCTGCGATCCAGATGTAAACGAAAATGTCTGTATTATTTCAGAAGGTTGCGAAGAGGTTGCTGGTGGGAGAGCTTCACATGATACGGATTCACCTTGTAAAGCCGACAAGGCGCTAGATTTGAAGAAGATGACACTTGGGGAGTGGTTTGATTATTTGGAGACTCATTTGCCCAAACAAATAATCGATGCAACAGAAGAAATAATTTCTAGTATGAGAATTAAATCTGCGCAAGTACGGGAGTACATCGCACGGCAGAAGAATGAGGAATGCAGGGGAGGTTAGTGGAAGGAAAACTCTGATGTACGAGGTAATCCTTTTGCCTCCTCATTTGATTTAAAATTTTGTATAGATCTCTGTTTGTTAATCATACCTTTCTGACCTTTTAATATATCCTCTATGACGTCCTGTGTAAAACATTGCTCTCATCTGTCAATCTATCTGGCATTGACTTTATGTGACTTAAGACTTGTATCCTTACTTAGATTAGGGACTGTAATCTGGGTGACATCAGTTTTCATTCATGGAAACGATGCAATGGCATGGTGATTTTATGGCTTTTAAACTCCACGAGGAGTATAATTTATGTAGAATCTATCAAAGAAGTTTGGGCTTGTGTATCAGGATTTTTTTTCAAGGAAATTAATTATTCAAGAATTTATCAGATCTGATGAGACAGTAGCTTAAACTAGTAGCAGAGGAAACAGTCAGTCTCTTTCTATTATGCAGAACTTAAACTCGTTGGATTGGACTTTCATCCTATCAAGAAACACTTGCTTGTTCATGGGACAGCTTGGAGAAATTCAAGAATAGAGAAAAGAGAGAGAAATTTGTGCAATTATTCATGGGACGATTCCACTGTTGTTGAGGGGAAAATCTTATGAATGCA

mRNA sequence

ATGGAATCCTCTGCAATTACTCTCATTATAGTTCAAGGCCCTCGCGAGGGCGAAACCCTAGAATTTCCATCCGGAACCACGATTCGGATTGGTCGCGTTGTTCGGGGTAACTCTATCGCCATTAAAGATGCTGGCATCTCCACCAAGCACCTTTCTATCCAACCTGAGCCTGTATCAGGTAAGTGGGTACTCCAGGACCTCGATTCGTCCAATGGCACATTTGTTAACGATACCAAGCTTCCTCCACACGATGCATTCGCTCTGAGCGACGGCGACACCATTAAATTCGGCGAGTGGACCTCGATTTTGGTCAGAATAAACACTAATGAAGAGTGCCGTTCGAGGCGAAACCCAAGGCGGAAAGCTGCAGAGAAGTGTACAGGTGCGGATGTGGTGGGATCGGTTGCGCAAACTCGGGGTCGAAGAGGAAAGGCTGTGGAAGAGGTCAGCGTACTTGGGGGAGGTAATGACGCGGGGTTGGAGAGTGGAAGATGTCTAAGGCCGAGGAGAGGCAGGGGTGTGAAAGCTGAAATTGATAACGAAGTACCGGATTGCAAAAAAATTGAGGACAATTTAGATGTGGGAAGAGGGTTAGAGAGTGTGACTGATCTGGAAAACGAACCCAGCCCAAAAATTACGGCAAGAAGTACCCGGAGAGCGAAGAATGATGTGCGTGTGGCTGCAGATTCAGTTATCGGGAAGGTTCCCGAGAATTCATGTGTGGGCGGTGAAGTAAAGGCAGGGGCAAAAAAGACGAGGGGAACTAGGGGAAGGAAGAAACTGCAAAATGAGCCGCCGGATTGCAGTACAGTTACAAAATTGGAACACACGGAAGCCATAGAGCAGAGGAGCTCAGGAGAAAATACCTTGGTTGACGATGAAGAAGGCAAGAAGAATGTAAACGTTGAAGAGACTGGTAGCGGGTCTTACCCTCAAGAGGCCTGCGATCCAGATGTAAACGAAAATGTCTGTATTATTTCAGAAGGTTGCGAAGAGGTTGCTGGTGGGAGAGCTTCACATGATACGGATTCACCTTGTAAAGCCGACAAGGCGCTAGATTTGAAGAAGATGACACTTGGGGAGTGGTTTGATTATTTGGAGACTCATTTGCCCAAACAAATAATCGATGCAACAGAAGAAATAATTTCTAGTATGAGAATTAAATCTGCGCAAGTACGGGAGTACATCGCACGGCAGAAGAATGAGGAATGCAGGGGAGGTTAG

Coding sequence (CDS)

ATGGAATCCTCTGCAATTACTCTCATTATAGTTCAAGGCCCTCGCGAGGGCGAAACCCTAGAATTTCCATCCGGAACCACGATTCGGATTGGTCGCGTTGTTCGGGGTAACTCTATCGCCATTAAAGATGCTGGCATCTCCACCAAGCACCTTTCTATCCAACCTGAGCCTGTATCAGGTAAGTGGGTACTCCAGGACCTCGATTCGTCCAATGGCACATTTGTTAACGATACCAAGCTTCCTCCACACGATGCATTCGCTCTGAGCGACGGCGACACCATTAAATTCGGCGAGTGGACCTCGATTTTGGTCAGAATAAACACTAATGAAGAGTGCCGTTCGAGGCGAAACCCAAGGCGGAAAGCTGCAGAGAAGTGTACAGGTGCGGATGTGGTGGGATCGGTTGCGCAAACTCGGGGTCGAAGAGGAAAGGCTGTGGAAGAGGTCAGCGTACTTGGGGGAGGTAATGACGCGGGGTTGGAGAGTGGAAGATGTCTAAGGCCGAGGAGAGGCAGGGGTGTGAAAGCTGAAATTGATAACGAAGTACCGGATTGCAAAAAAATTGAGGACAATTTAGATGTGGGAAGAGGGTTAGAGAGTGTGACTGATCTGGAAAACGAACCCAGCCCAAAAATTACGGCAAGAAGTACCCGGAGAGCGAAGAATGATGTGCGTGTGGCTGCAGATTCAGTTATCGGGAAGGTTCCCGAGAATTCATGTGTGGGCGGTGAAGTAAAGGCAGGGGCAAAAAAGACGAGGGGAACTAGGGGAAGGAAGAAACTGCAAAATGAGCCGCCGGATTGCAGTACAGTTACAAAATTGGAACACACGGAAGCCATAGAGCAGAGGAGCTCAGGAGAAAATACCTTGGTTGACGATGAAGAAGGCAAGAAGAATGTAAACGTTGAAGAGACTGGTAGCGGGTCTTACCCTCAAGAGGCCTGCGATCCAGATGTAAACGAAAATGTCTGTATTATTTCAGAAGGTTGCGAAGAGGTTGCTGGTGGGAGAGCTTCACATGATACGGATTCACCTTGTAAAGCCGACAAGGCGCTAGATTTGAAGAAGATGACACTTGGGGAGTGGTTTGATTATTTGGAGACTCATTTGCCCAAACAAATAATCGATGCAACAGAAGAAATAATTTCTAGTATGAGAATTAAATCTGCGCAAGTACGGGAGTACATCGCACGGCAGAAGAATGAGGAATGCAGGGGAGGTTAG

Protein sequence

MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSGKWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRRKAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDNEVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSCVGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG
Homology
BLAST of MC05g_new0004 vs. ExPASy Swiss-Prot
Match: O23305 (FHA domain-containing protein At4g14490 OS=Arabidopsis thaliana OX=3702 GN=At4g14490 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 5.2e-40
Identity = 147/440 (33.41%), Postives = 221/440 (50.23%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           M + ++ L+ V+GPREG+ L++  G+TIR+GR+VRGN IAIKDAGISTKHL I+ +  SG
Sbjct: 1   MVTPSLRLVFVKGPREGDALDYKPGSTIRVGRIVRGNEIAIKDAGISTKHLRIESD--SG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVR--INTNEECRSRRNP 120
            WV+QDL SSNGT +N   L P  +  L DGD IK GE+TSILV   I+  +E +  RN 
Sbjct: 61  NWVIQDLGSSNGTLLNSNALDPETSVNLGDGDVIKLGEYTSILVNFVIDDFQEKKLTRNN 120

Query: 121 RRKA-----------------AEKCTGADV-VGSVAQTRGRRGKAVEEVSVLGGGNDAGL 180
           RR+A                  E+  G DV   +   +R R+ + +E+   LG  +  GL
Sbjct: 121 RRQANARKRIRVLESINLGDITEEEKGLDVKFENKPSSRVRKVRKIEDSEKLGITD--GL 180

Query: 181 ESGRCLRPRRGRGVKA-------EIDNEVPDCKKIEDNLDVGRGLESVTDLENEPSPKIT 240
           +     +    R V++        I  E+ DC  +E+NL  GRGL+           +++
Sbjct: 181 QEDLVEKNGSFRNVESIQSSSVNLIKVEMEDCAMVEENL--GRGLKK----------RVS 240

Query: 241 ARSTRRAKNDVRVAADSVIGKVPENSCVG------GEVKAGAKKTRGTRGRKKLQNEPPD 300
           +++TR  K       +  +GK    +C+G       E     + TR TR +K       D
Sbjct: 241 SKATRSKK------IEESVGK----ACLGVVNVEKVETLKEKRITRATRSKK------ID 300

Query: 301 CSTVTKLEHTEAIE--QRSSGENTLVDDEEGKKNVNVEETGSGSYPQEACDPDVNENVCI 360
               + LE    +   +++ G+N   D +  K   N E T SG   ++     V E++  
Sbjct: 301 IVGDSYLELDMVLNRARKNKGKNKKADQKPLKSFENDEVTDSG---EQGLSCHVEEDM-- 360

Query: 361 ISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLGEWFDYLETHLPKQIIDATEEIISSM 406
                        +++ D+        DL+KMTLG  F +LE HL K+II  TE +I  M
Sbjct: 361 -------------NNELDT--------DLRKMTLGALFSFLEGHLSKEIIHKTENMIEPM 382

BLAST of MC05g_new0004 vs. ExPASy Swiss-Prot
Match: P46014 (Protein phosphatase 2C 70 OS=Arabidopsis thaliana OX=3702 GN=KAPP PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 4.1e-05
Identity = 36/122 (29.51%), Postives = 56/122 (45.90%), Query Frame = 0

Query: 2   ESSAITLIIVQGPREGETLEFPSGTT----IRIGRVVRGNSIAIKDAGISTKHLSIQPEP 61
           + S + L ++ GP  G      S ++    +++GR V  + +A+KD+ +S KH  I    
Sbjct: 178 QRSWLFLEVIAGPAIGLQHAVNSTSSSKLPVKLGR-VSPSDLALKDSEVSGKHAQITWNS 237

Query: 62  VSGKWVLQDLDSSNGTFVNDTKLPPHD--------AFALSDGDTIKFGEWTSILVRINTN 112
              KW L D+ S NGT VN   +   D           L+  D I  G  T + VRI++ 
Sbjct: 238 TKFKWELVDMGSLNGTLVNSHSISHPDLGSRKWGNPVELASDDIITLGTTTKVYVRISSQ 297

BLAST of MC05g_new0004 vs. ExPASy Swiss-Prot
Match: Q03944 (Vacuolar protein sorting-associated protein 64 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS64 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 4.6e-04
Identity = 23/52 (44.23%), Postives = 31/52 (59.62%), Query Frame = 0

Query: 46  ISTKHLSIQPEPVSGKWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFG 98
           +S  H  +  +P SGK  ++DL SSNGTFVN  K+  +D   L  GDT+  G
Sbjct: 223 LSRNHACLSCDPTSGKIYIRDLKSSNGTFVNGVKIRQNDV-ELKVGDTVDLG 273

BLAST of MC05g_new0004 vs. NCBI nr
Match: XP_022145157.1 (FHA domain-containing protein At4g14490-like [Momordica charantia])

HSP 1 Score: 809 bits (2089), Expect = 2.19e-295
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG
Sbjct: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR
Sbjct: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN
Sbjct: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC
Sbjct: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240

Query: 241 VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV 300
           VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV
Sbjct: 241 VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV 300

Query: 301 NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG 360
           NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG
Sbjct: 301 NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG 360

Query: 361 EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG
Sbjct: 361 EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407

BLAST of MC05g_new0004 vs. NCBI nr
Match: XP_038877177.1 (FHA domain-containing protein At4g14490-like [Benincasa hispida] >XP_038877178.1 FHA domain-containing protein At4g14490-like [Benincasa hispida] >XP_038877179.1 FHA domain-containing protein At4g14490-like [Benincasa hispida] >XP_038877180.1 FHA domain-containing protein At4g14490-like [Benincasa hispida] >XP_038877182.1 FHA domain-containing protein At4g14490-like [Benincasa hispida])

HSP 1 Score: 622 bits (1605), Expect = 1.18e-221
Identity = 321/409 (78.48%), Postives = 351/409 (85.82%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+V+GPREGETL+FP GTTIRIGR VRGNS+AIKDAGISTKH SI+ E VSG
Sbjct: 1   MESSAITLIMVKGPREGETLDFPPGTTIRIGRTVRGNSVAIKDAGISTKHFSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+L+DLDSSNGTFVNDTKLPPHDAF LSDGD IK GE TSILVRIN+NEEC SRRNPRR
Sbjct: 61  KWMLRDLDSSNGTFVNDTKLPPHDAFDLSDGDIIKCGELTSILVRINSNEECPSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAA+KCT +D V SVA TRGRRGK VEEV  +GG ND  +ESGRCLR R+GRGVK EI N
Sbjct: 121 KAADKCTSSDAVVSVAGTRGRRGKVVEEV--VGGCNDTMIESGRCLRSRKGRGVKGEIAN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +VPDCKKIEDNLDVGRG E+V ++ NEP PKITARSTRR KN V VA DSV+G VPENSC
Sbjct: 181 QVPDCKKIEDNLDVGRGSENVNNVVNEPGPKITARSTRRTKNTVCVATDSVLGNVPENSC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           VG EVKAGAKKTR GTRGRKKLQNEPP DC+TV KLEH+E +EQ+S GEN LVDD EG+K
Sbjct: 241 VGDEVKAGAKKTRAGTRGRKKLQNEPPPDCNTVIKLEHSENVEQKSLGENKLVDDGEGEK 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N NVEE GSGS PQE CD D N++VCIISEGCEEVA G ASHD    CKA+K  DLKKMT
Sbjct: 301 NANVEERGSGSSPQEVCDRDENQDVCIISEGCEEVADGIASHDEGFLCKAEKVPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLP+QIIDATEEIIS MRIKS QVREY+A+QK E C+GG
Sbjct: 361 LGEWFDYLETHLPRQIIDATEEIISGMRIKSKQVREYVAQQKIENCQGG 407

BLAST of MC05g_new0004 vs. NCBI nr
Match: XP_023526744.1 (FHA domain-containing protein At4g14490-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614 bits (1584), Expect = 1.99e-218
Identity = 318/409 (77.75%), Postives = 353/409 (86.31%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+VQGPREGETL FP GTTIRIGRVVRGNS+AIKDAGISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVQGPREGETLHFPPGTTIRIGRVVRGNSVAIKDAGISTKHLSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+L+DLDSSNGTFVND KL PHDA AL DGDTIK GE TSILVRIN+NEECRS+RNPRR
Sbjct: 61  KWMLRDLDSSNGTFVNDAKLSPHDAVALRDGDTIKCGELTSILVRINSNEECRSKRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTG++VVGSVA TRGRRGKAVEEVSVLG  N+A LESGRCLRPR+ RGVK EI N
Sbjct: 121 KAAEKCTGSNVVGSVAGTRGRRGKAVEEVSVLGVFNEATLESGRCLRPRKRRGVKCEITN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +V DCKKIE+NLD+ RG E+V ++ENEPS KIT  STR+A + VRVA DSV+G VPEN C
Sbjct: 181 QVADCKKIEENLDLVRGSENVNNVENEPSTKITRGSTRKANSTVRVATDSVLGNVPENPC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           +GGE+KAGAKKTR GTRGRKKLQNEPP DC TV KLEH+E++EQ+S GEN LVDD EGK 
Sbjct: 241 LGGELKAGAKKTRAGTRGRKKLQNEPPPDCGTVIKLEHSESVEQKSLGENKLVDDGEGKN 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N +VEE GSGS PQ  CD D NE+VCIISEGCE+VA GR SH  D   KA++A DLKKMT
Sbjct: 301 NAHVEEAGSGSSPQVVCDRDENEDVCIISEGCEKVADGRDSHGEDILRKAEEAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLPKQIIDATEE+IS MRIKSAQVREY+A+QK E  +GG
Sbjct: 361 LGEWFDYLETHLPKQIIDATEEVISGMRIKSAQVREYVAQQKIENSQGG 409

BLAST of MC05g_new0004 vs. NCBI nr
Match: XP_022924382.1 (FHA domain-containing protein At4g14490-like [Cucurbita moschata])

HSP 1 Score: 612 bits (1578), Expect = 1.63e-217
Identity = 318/409 (77.75%), Postives = 351/409 (85.82%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+VQGPREGETL FP GTTIRIGRVVRGNS+AIKDA ISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVQGPREGETLHFPPGTTIRIGRVVRGNSVAIKDAAISTKHLSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+L+DLDSSNGTFVND KL PHDA ALSDGDTIK GE TSILVRIN NEECRS+RNPRR
Sbjct: 61  KWMLRDLDSSNGTFVNDAKLSPHDAVALSDGDTIKCGELTSILVRINGNEECRSKRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTG++VVGSVA TRGRRGKAV EVSVLG  N+A LESGRCLRPR+ RGVK EI N
Sbjct: 121 KAAEKCTGSNVVGSVAGTRGRRGKAVGEVSVLGVFNEATLESGRCLRPRKRRGVKCEITN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +V DCKKIE+NLD+ RG E+V ++ENEPS KIT  STR+A + VRVA DSV+G VPEN C
Sbjct: 181 QVADCKKIEENLDLVRGSENVNNVENEPSTKITRGSTRKANSTVRVATDSVLGNVPENPC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           +GGE+KAGAKKTR GTRGRKKLQNEPP DC TV KLEH+E++EQ+S GEN LVDD EGK 
Sbjct: 241 LGGELKAGAKKTRAGTRGRKKLQNEPPPDCGTVIKLEHSESVEQKSLGENKLVDDGEGKN 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N +VEE GSGS PQ  CD D NE+VCIISEGCE+VA GR SH  D   KA++A DLKKMT
Sbjct: 301 NAHVEEAGSGSSPQVVCDRDENEDVCIISEGCEKVADGRDSHGEDIRRKAEEAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLPKQIIDATEE+IS MRIKSAQVREY+A+QK E  RGG
Sbjct: 361 LGEWFDYLETHLPKQIIDATEEVISGMRIKSAQVREYVAQQKIENSRGG 409

BLAST of MC05g_new0004 vs. NCBI nr
Match: KAG6582363.1 (FHA domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 610 bits (1573), Expect = 9.40e-217
Identity = 316/409 (77.26%), Postives = 353/409 (86.31%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI++QGPREGETL FP GTTIRIGRVVRGNS+AIKDAGISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMLQGPREGETLHFPPGTTIRIGRVVRGNSVAIKDAGISTKHLSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+L+DLDSSNGTFVND KL P DA ALSDGDTIK GE TSILVRIN+NEECRS+RNPRR
Sbjct: 61  KWMLRDLDSSNGTFVNDAKLSPQDAVALSDGDTIKCGELTSILVRINSNEECRSKRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTG++VVGSVA TRGRRGKAVEEVSVLG  N+A LESGRCLRPR+ RGVK EI N
Sbjct: 121 KAAEKCTGSNVVGSVAGTRGRRGKAVEEVSVLGVFNEATLESGRCLRPRKRRGVKCEITN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +V DCKKIE+NLD+ RG E+V ++ENEPS KIT  STR+A + VRVA DSV+G VPEN C
Sbjct: 181 QVADCKKIEENLDLVRGSENVNNVENEPSIKITRGSTRKANSTVRVATDSVLGNVPENPC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           +GGE+KAGAK+TR GTRGRKKLQNEPP DC TV KLEH+E++EQ+S GEN LVDD EGK 
Sbjct: 241 LGGELKAGAKRTRAGTRGRKKLQNEPPPDCGTVIKLEHSESVEQKSLGENKLVDDGEGKN 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N +VEE GSGS PQ  CD D NE+VCIISEGCE+VA GR SH  D   KA++A DLKKMT
Sbjct: 301 NAHVEEAGSGSSPQVVCDRDENEDVCIISEGCEKVADGRDSHGEDILRKAEEAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLPKQIIDATEE+IS MRIKSAQVREY+A+QK E  +GG
Sbjct: 361 LGEWFDYLETHLPKQIIDATEEVISGMRIKSAQVREYVAQQKIENSQGG 409

BLAST of MC05g_new0004 vs. ExPASy TrEMBL
Match: A0A6J1CVP7 (FHA domain-containing protein At4g14490-like OS=Momordica charantia OX=3673 GN=LOC111014670 PE=4 SV=1)

HSP 1 Score: 809 bits (2089), Expect = 1.06e-295
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG
Sbjct: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR
Sbjct: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN
Sbjct: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC
Sbjct: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240

Query: 241 VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV 300
           VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV
Sbjct: 241 VGGEVKAGAKKTRGTRGRKKLQNEPPDCSTVTKLEHTEAIEQRSSGENTLVDDEEGKKNV 300

Query: 301 NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG 360
           NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG
Sbjct: 301 NVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLG 360

Query: 361 EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG
Sbjct: 361 EWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407

BLAST of MC05g_new0004 vs. ExPASy TrEMBL
Match: A0A6J1E8S0 (FHA domain-containing protein At4g14490-like OS=Cucurbita moschata OX=3662 GN=LOC111431894 PE=4 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 7.89e-218
Identity = 318/409 (77.75%), Postives = 351/409 (85.82%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+VQGPREGETL FP GTTIRIGRVVRGNS+AIKDA ISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVQGPREGETLHFPPGTTIRIGRVVRGNSVAIKDAAISTKHLSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+L+DLDSSNGTFVND KL PHDA ALSDGDTIK GE TSILVRIN NEECRS+RNPRR
Sbjct: 61  KWMLRDLDSSNGTFVNDAKLSPHDAVALSDGDTIKCGELTSILVRINGNEECRSKRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTG++VVGSVA TRGRRGKAV EVSVLG  N+A LESGRCLRPR+ RGVK EI N
Sbjct: 121 KAAEKCTGSNVVGSVAGTRGRRGKAVGEVSVLGVFNEATLESGRCLRPRKRRGVKCEITN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +V DCKKIE+NLD+ RG E+V ++ENEPS KIT  STR+A + VRVA DSV+G VPEN C
Sbjct: 181 QVADCKKIEENLDLVRGSENVNNVENEPSTKITRGSTRKANSTVRVATDSVLGNVPENPC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           +GGE+KAGAKKTR GTRGRKKLQNEPP DC TV KLEH+E++EQ+S GEN LVDD EGK 
Sbjct: 241 LGGELKAGAKKTRAGTRGRKKLQNEPPPDCGTVIKLEHSESVEQKSLGENKLVDDGEGKN 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N +VEE GSGS PQ  CD D NE+VCIISEGCE+VA GR SH  D   KA++A DLKKMT
Sbjct: 301 NAHVEEAGSGSSPQVVCDRDENEDVCIISEGCEKVADGRDSHGEDIRRKAEEAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLPKQIIDATEE+IS MRIKSAQVREY+A+QK E  RGG
Sbjct: 361 LGEWFDYLETHLPKQIIDATEEVISGMRIKSAQVREYVAQQKIENSRGG 409

BLAST of MC05g_new0004 vs. ExPASy TrEMBL
Match: A0A6J1J0B7 (FHA domain-containing protein At4g14490-like OS=Cucurbita maxima OX=3661 GN=LOC111480104 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 4.33e-215
Identity = 312/409 (76.28%), Postives = 349/409 (85.33%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+VQGPREGETL FP GTTIRIGRVVRGNS+AIKD GISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVQGPREGETLHFPPGTTIRIGRVVRGNSVAIKDVGISTKHLSIESESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
           KW+++DLDSSNGTFVNDTKL PHDA ALSDGDTIK GE TSILVRIN+NE+CRSRRNPRR
Sbjct: 61  KWMIRDLDSSNGTFVNDTKLSPHDAVALSDGDTIKCGELTSILVRINSNEDCRSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCTG++VVGSVA TRGRRGKAVEEVS+LG  N+A LESGRCLRPR+GRGVK EI N
Sbjct: 121 KAAEKCTGSNVVGSVAGTRGRRGKAVEEVSILGVFNEATLESGRCLRPRKGRGVKCEITN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +V DCKKIE NLD  RG E+V ++ENEPS KIT  ST +A + VRVA DSV+G VPEN C
Sbjct: 181 QVADCKKIEKNLDFVRGSENVNNVENEPSTKITRGSTMKANSTVRVATDSVLGNVPENPC 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           +GGE+KAG K+TR GTRGRKKLQNEPP DC TV KLEH+E++EQ+S GEN LV D EGK 
Sbjct: 241 LGGELKAGEKRTRAGTRGRKKLQNEPPPDCGTVIKLEHSESVEQKSLGENKLVVDGEGKN 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N +VEE GSGS PQ  CD D NE+VCIISEGCE+VA GR SH  D   K ++A DLKKMT
Sbjct: 301 NAHVEEAGSGSSPQVVCDRDENEDVCIISEGCEKVADGRDSHGEDILRKVEEAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRGG 407
           LGEWFDYLETHLP+QIIDATEE+IS MRIKSA+VREY+A+QK E  RGG
Sbjct: 361 LGEWFDYLETHLPRQIIDATEEVISGMRIKSARVREYVAQQKIENSRGG 409

BLAST of MC05g_new0004 vs. ExPASy TrEMBL
Match: A0A5D3D3C5 (FHA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001220 PE=4 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 5.56e-211
Identity = 309/408 (75.74%), Postives = 345/408 (84.56%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+V+GPREGETL+FP G TIRIGR VRGNS+AIKDAGISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVKGPREGETLDFPPGATIRIGRTVRGNSVAIKDAGISTKHLSIEFESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
            W+L+DLDSSNGTFVND KLPPHDAFAL+DGDTIK GE TSI VRIN+NEE RSRRNPRR
Sbjct: 61  NWMLRDLDSSNGTFVNDIKLPPHDAFALNDGDTIKCGELTSIFVRINSNEEPRSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCT +DVVGSVA TRGRRGK VEEVSV+GG NDA  +SGRCLR R+GRGVK EIDN
Sbjct: 121 KAAEKCTSSDVVGSVAGTRGRRGKVVEEVSVVGGCNDAMGDSGRCLRSRKGRGVKDEIDN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +VPDCKKI D LDVGR  +++ +  NEP PKI  RSTRR KN V VA DSV+  VPE+S 
Sbjct: 181 QVPDCKKIGDKLDVGRESQNMNNAVNEPGPKIAKRSTRRTKNTVSVATDSVLENVPEDSR 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           VGGEVKA AKKTR GTRGRKKLQNEPP + STV KLEH+E +E++S GEN LVD  EGKK
Sbjct: 241 VGGEVKAEAKKTRAGTRGRKKLQNEPPLEISTVIKLEHSENVEEKSLGENKLVDVGEGKK 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N NVEE   GS PQE CD D N++VCIISEGCEEVA GRASHD D  CK++KA DLKKMT
Sbjct: 301 NANVEERCCGSSPQEICDRDGNQDVCIISEGCEEVADGRASHDEDFLCKSEKAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRG 406
           LG+WFDYLETHLP+QIIDATEE+IS M+IKS QV+EY+ARQK E C+G
Sbjct: 361 LGDWFDYLETHLPRQIIDATEEVISGMKIKSKQVQEYVARQKIENCQG 408

BLAST of MC05g_new0004 vs. ExPASy TrEMBL
Match: A0A1S3AWC3 (FHA domain-containing protein At4g14490-like OS=Cucumis melo OX=3656 GN=LOC103483633 PE=4 SV=1)

HSP 1 Score: 592 bits (1526), Expect = 6.45e-210
Identity = 308/408 (75.49%), Postives = 343/408 (84.07%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           MESSAITLI+V+GPREGETL+FP G TIRIGR VRGNS+AIKDAGISTKHLSI+ E VSG
Sbjct: 1   MESSAITLIMVKGPREGETLDFPPGATIRIGRTVRGNSVAIKDAGISTKHLSIEFESVSG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNEECRSRRNPRR 120
            W+L+DLDSSNGTFVND KLPPHDAFAL+DGDTIK GE TSI VRIN+NEE RSRRNPRR
Sbjct: 61  NWMLRDLDSSNGTFVNDIKLPPHDAFALNDGDTIKCGELTSIFVRINSNEEPRSRRNPRR 120

Query: 121 KAAEKCTGADVVGSVAQTRGRRGKAVEEVSVLGGGNDAGLESGRCLRPRRGRGVKAEIDN 180
           KAAEKCT +DVVGSVA TRGRRGK VEEVSV+GG NDA  +SGRCLR R+GRGVK EIDN
Sbjct: 121 KAAEKCTSSDVVGSVAGTRGRRGKVVEEVSVVGGCNDAMGDSGRCLRSRKGRGVKDEIDN 180

Query: 181 EVPDCKKIEDNLDVGRGLESVTDLENEPSPKITARSTRRAKNDVRVAADSVIGKVPENSC 240
           +VPDCKKI D LDVGR  +++ +  NEP PKI  RSTRR KN V VA DSV+  VPE+S 
Sbjct: 181 QVPDCKKIGDKLDVGRESQNMNNAVNEPGPKIAKRSTRRTKNTVSVATDSVLENVPEDSR 240

Query: 241 VGGEVKAGAKKTR-GTRGRKKLQNEPP-DCSTVTKLEHTEAIEQRSSGENTLVDDEEGKK 300
           VGGEVKA AKKTR GTRGRKKLQNEPP + STV KLEH E +E++S GEN LVD  EGKK
Sbjct: 241 VGGEVKAEAKKTRAGTRGRKKLQNEPPLEISTVIKLEHIENVEEKSLGENKLVDVGEGKK 300

Query: 301 NVNVEETGSGSYPQEACDPDVNENVCIISEGCEEVAGGRASHDTDSPCKADKALDLKKMT 360
           N NVEE   GS P E CD D N++VCIISEGCEEVA GRASHD D  CK++KA DLKKMT
Sbjct: 301 NANVEEICCGSSPHEICDRDGNQDVCIISEGCEEVADGRASHDEDFLCKSEKAPDLKKMT 360

Query: 361 LGEWFDYLETHLPKQIIDATEEIISSMRIKSAQVREYIARQKNEECRG 406
           LG+WFDYLETHLP+QIIDATEE+IS M+IKS QV+EY+ARQK E C+G
Sbjct: 361 LGDWFDYLETHLPRQIIDATEEVISGMKIKSKQVQEYVARQKIENCQG 408

BLAST of MC05g_new0004 vs. TAIR 10
Match: AT4G14490.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.7e-41
Identity = 147/440 (33.41%), Postives = 221/440 (50.23%), Query Frame = 0

Query: 1   MESSAITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSG 60
           M + ++ L+ V+GPREG+ L++  G+TIR+GR+VRGN IAIKDAGISTKHL I+ +  SG
Sbjct: 1   MVTPSLRLVFVKGPREGDALDYKPGSTIRVGRIVRGNEIAIKDAGISTKHLRIESD--SG 60

Query: 61  KWVLQDLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVR--INTNEECRSRRNP 120
            WV+QDL SSNGT +N   L P  +  L DGD IK GE+TSILV   I+  +E +  RN 
Sbjct: 61  NWVIQDLGSSNGTLLNSNALDPETSVNLGDGDVIKLGEYTSILVNFVIDDFQEKKLTRNN 120

Query: 121 RRKA-----------------AEKCTGADV-VGSVAQTRGRRGKAVEEVSVLGGGNDAGL 180
           RR+A                  E+  G DV   +   +R R+ + +E+   LG  +  GL
Sbjct: 121 RRQANARKRIRVLESINLGDITEEEKGLDVKFENKPSSRVRKVRKIEDSEKLGITD--GL 180

Query: 181 ESGRCLRPRRGRGVKA-------EIDNEVPDCKKIEDNLDVGRGLESVTDLENEPSPKIT 240
           +     +    R V++        I  E+ DC  +E+NL  GRGL+           +++
Sbjct: 181 QEDLVEKNGSFRNVESIQSSSVNLIKVEMEDCAMVEENL--GRGLKK----------RVS 240

Query: 241 ARSTRRAKNDVRVAADSVIGKVPENSCVG------GEVKAGAKKTRGTRGRKKLQNEPPD 300
           +++TR  K       +  +GK    +C+G       E     + TR TR +K       D
Sbjct: 241 SKATRSKK------IEESVGK----ACLGVVNVEKVETLKEKRITRATRSKK------ID 300

Query: 301 CSTVTKLEHTEAIE--QRSSGENTLVDDEEGKKNVNVEETGSGSYPQEACDPDVNENVCI 360
               + LE    +   +++ G+N   D +  K   N E T SG   ++     V E++  
Sbjct: 301 IVGDSYLELDMVLNRARKNKGKNKKADQKPLKSFENDEVTDSG---EQGLSCHVEEDM-- 360

Query: 361 ISEGCEEVAGGRASHDTDSPCKADKALDLKKMTLGEWFDYLETHLPKQIIDATEEIISSM 406
                        +++ D+        DL+KMTLG  F +LE HL K+II  TE +I  M
Sbjct: 361 -------------NNELDT--------DLRKMTLGALFSFLEGHLSKEIIHKTENMIEPM 382

BLAST of MC05g_new0004 vs. TAIR 10
Match: AT3G02400.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 136.7 bits (343), Expect = 4.1e-32
Identity = 166/587 (28.28%), Postives = 220/587 (37.48%), Query Frame = 0

Query: 6   ITLIIVQGPREGETLEFPSGTTIRIGRVVRGNSIAIKDAGISTKHLSIQPEPVSGKWVLQ 65
           + L   QGPR G++L F  G+TIRIGR+VRGN IAIKDAGISTKHL I  +  S  W++ 
Sbjct: 6   LKLDFTQGPRAGDSLGFKPGSTIRIGRIVRGNEIAIKDAGISTKHLRIVSD--SENWIIH 65

Query: 66  DLDSSNGTFVNDTKLPPHDAFALSDGDTIKFGEWTSILVRINTNE---------ECRSRR 125
           DL SSNGT +N   +       LS GD IK GE+TSILV   ++            R RR
Sbjct: 66  DLGSSNGTILNSDTIDSDTPVNLSHGDEIKLGEYTSILVNFGSDVVQAPQEHKLPPRPRR 125

Query: 126 NPRRKAAEKCTGADVVGSVAQ-------------------TRGRRGKAVEEVSVLGGGND 185
           N +R AA      D + SV +                   TR  R K +++++      D
Sbjct: 126 NNKRLAASD-PDPDPIESVQEKPKRTRGSSKQEENELPKSTRASRKKNLDDIADKEEELD 185

Query: 186 AGLE---SGRCLRPRRGRGVKAEIDNEVPDCKKIEDNLDVGR----GLESVTDLENEPSP 245
             +E     R  RPR+  G     + EV     +E+   VGR       ++T+ E     
Sbjct: 186 VEIEKVVKARVGRPRKNAGSAIAKEEEV-----VEEKKRVGRPRKNASSAITEEEEVVEE 245

Query: 246 KITARSTRRAKN----------------------DVRVAADSVIGKVPENSCVGGEVKAG 305
           K      RR KN                      +V+        K  EN C G EVK  
Sbjct: 246 KKGNSRARRGKNSEIVQKSIKLEVEDTPKAVEISEVKSRKRVTRSKQIENECFGLEVKDE 305

Query: 306 AKKTRGTRG--------------------------RKKLQNEP--------------PDC 365
            + TR TR                           RKK+  EP               +C
Sbjct: 306 KRTTRSTRSKTTEIGGESFLELEMVLNQARKSRAKRKKMDEEPSKETRNDDAGEEVLKNC 365

Query: 366 STVTKLEHTEAIEQRSSGENTLVDDEEGK--------KNVNVE---------------ET 403
             V + +  EA E  S   + + D E+ K        + V +E               E 
Sbjct: 366 H-VEEDKENEAQEGCSGRSDDICDQEDEKECDGSKRVEQVEIELRKKSTVEGLKCTDKED 425

BLAST of MC05g_new0004 vs. TAIR 10
Match: AT5G19280.1 (kinase associated protein phosphatase )

HSP 1 Score: 50.8 bits (120), Expect = 2.9e-06
Identity = 36/122 (29.51%), Postives = 56/122 (45.90%), Query Frame = 0

Query: 2   ESSAITLIIVQGPREGETLEFPSGTT----IRIGRVVRGNSIAIKDAGISTKHLSIQPEP 61
           + S + L ++ GP  G      S ++    +++GR V  + +A+KD+ +S KH  I    
Sbjct: 178 QRSWLFLEVIAGPAIGLQHAVNSTSSSKLPVKLGR-VSPSDLALKDSEVSGKHAQITWNS 237

Query: 62  VSGKWVLQDLDSSNGTFVNDTKLPPHD--------AFALSDGDTIKFGEWTSILVRINTN 112
              KW L D+ S NGT VN   +   D           L+  D I  G  T + VRI++ 
Sbjct: 238 TKFKWELVDMGSLNGTLVNSHSISHPDLGSRKWGNPVELASDDIITLGTTTKVYVRISSQ 297

BLAST of MC05g_new0004 vs. TAIR 10
Match: AT5G19280.2 (kinase associated protein phosphatase )

HSP 1 Score: 50.8 bits (120), Expect = 2.9e-06
Identity = 36/122 (29.51%), Postives = 56/122 (45.90%), Query Frame = 0

Query: 2   ESSAITLIIVQGPREGETLEFPSGTT----IRIGRVVRGNSIAIKDAGISTKHLSIQPEP 61
           + S + L ++ GP  G      S ++    +++GR V  + +A+KD+ +S KH  I    
Sbjct: 178 QRSWLFLEVIAGPAIGLQHAVNSTSSSKLPVKLGR-VSPSDLALKDSEVSGKHAQITWNS 237

Query: 62  VSGKWVLQDLDSSNGTFVNDTKLPPHD--------AFALSDGDTIKFGEWTSILVRINTN 112
              KW L D+ S NGT VN   +   D           L+  D I  G  T + VRI++ 
Sbjct: 238 TKFKWELVDMGSLNGTLVNSHSISHPDLGSRKWGNPVELASDDIITLGTTTKVYVRISSQ 297

BLAST of MC05g_new0004 vs. TAIR 10
Match: AT1G34355.1 (forkhead-associated (FHA) domain-containing protein )

HSP 1 Score: 46.2 bits (108), Expect = 7.2e-05
Identity = 30/90 (33.33%), Postives = 41/90 (45.56%), Query Frame = 0

Query: 15  REGETLEFPSGTTIRIGRVVR--GNSIAIKDAGISTKHLSIQPEPVSGKWVLQDLDSSNG 74
           R G T+    G    I  V R     I +    IS  HL I+      K  + DL S +G
Sbjct: 48  RNGSTVSDDDGEVEEILVVGRHPDCDILLTHPSISRFHLEIRSISSRQKLFVTDLSSVHG 107

Query: 75  TFVNDTKLPPHDAFALSDGDTIKFGEWTSI 103
           T+V D ++ PH    + +GDTI+ G  T I
Sbjct: 108 TWVRDLRIEPHGCVEVEEGDTIRIGGSTRI 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O233055.2e-4033.41FHA domain-containing protein At4g14490 OS=Arabidopsis thaliana OX=3702 GN=At4g1... [more]
P460144.1e-0529.51Protein phosphatase 2C 70 OS=Arabidopsis thaliana OX=3702 GN=KAPP PE=1 SV=2[more]
Q039444.6e-0444.23Vacuolar protein sorting-associated protein 64 OS=Saccharomyces cerevisiae (stra... [more]
Match NameE-valueIdentityDescription
XP_022145157.12.19e-295100.00FHA domain-containing protein At4g14490-like [Momordica charantia][more]
XP_038877177.11.18e-22178.48FHA domain-containing protein At4g14490-like [Benincasa hispida] >XP_038877178.1... [more]
XP_023526744.11.99e-21877.75FHA domain-containing protein At4g14490-like [Cucurbita pepo subsp. pepo][more]
XP_022924382.11.63e-21777.75FHA domain-containing protein At4g14490-like [Cucurbita moschata][more]
KAG6582363.19.40e-21777.26FHA domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1CVP71.06e-295100.00FHA domain-containing protein At4g14490-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1E8S07.89e-21877.75FHA domain-containing protein At4g14490-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J0B74.33e-21576.28FHA domain-containing protein At4g14490-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5D3D3C55.56e-21175.74FHA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3AWC36.45e-21075.49FHA domain-containing protein At4g14490-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT4G14490.13.7e-4133.41SMAD/FHA domain-containing protein [more]
AT3G02400.14.1e-3228.28SMAD/FHA domain-containing protein [more]
AT5G19280.12.9e-0629.51kinase associated protein phosphatase [more]
AT5G19280.22.9e-0629.51kinase associated protein phosphatase [more]
AT1G34355.17.2e-0533.33forkhead-associated (FHA) domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000253Forkhead-associated (FHA) domainSMARTSM00240FHA_2coord: 27..80
e-value: 5.4E-12
score: 55.9
IPR000253Forkhead-associated (FHA) domainPFAMPF00498FHAcoord: 29..97
e-value: 3.7E-17
score: 62.4
IPR000253Forkhead-associated (FHA) domainPROSITEPS50006FHA_DOMAINcoord: 28..80
score: 13.7877
IPR000253Forkhead-associated (FHA) domainCDDcd00060FHAcoord: 6..108
e-value: 9.24135E-25
score: 95.531
NoneNo IPR availableGENE3D2.60.200.20coord: 1..112
e-value: 1.8E-30
score: 107.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 274..301
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..314
NoneNo IPR availablePANTHERPTHR23308NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1coord: 1..328
NoneNo IPR availablePANTHERPTHR23308:SF53F16B3.3 PROTEINcoord: 1..328
IPR008984SMAD/FHA domain superfamilySUPERFAMILY49879SMAD/FHA domaincoord: 5..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g_new0004.1MC05g_new0004.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003729 mRNA binding
molecular_function GO:0005515 protein binding